Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| association of body cavity-based lymphoma and human herpesvirus 8 in an hiv-seronegative male. report of a case with immunocytochemical and molecular studies. | recently lymphomas arising primarily in serosal surfaces have been found in patients with advanced acquired immunodeficiency syndrome (aids), but they very rarely seem to occur in human immunodeficiency virus (hiv)-negative patients. studies on a subset of these lymphomas suggested that they represent a distinct entity associated with kaposi's sarcoma-associated herpesvirus or human herpesvirus 8 (hhv-8). | 1999 | 10097730 |
| aids-associated malignancies: research perspectives. | the appearance in 1981 of a usually rare malignancy, kaposi's sarcoma, in homosexual men [1] was one of the first harbingers of an epidemic caused by a retrovirus, human immunodeficiency virus (hiv), which causes the acquired immunodeficiency syndrome (aids). lymphoid and other malignancies were also increased, most strikingly non-hodgkin's lymphoma and primary central nervous system (cns) lymphoma. advances in molecular biology, immunology, virology, and anti-viral therapy have combined to crea ... | 1999 | 9989205 |
| initiation and maintenance of multiple myeloma. | a recently identified herpesvirus, human herpesvirus-8 (hhv-8), also known as kaposi's sarcoma (ks)-associated herpesvirus, has been found in nonmalignant bone marrow dendritic cells of patients with multiple myeloma. the virus is also detectable in the peripheral blood of most patients; its absence suggests earlier-stage disease. hhv-8 is not detected in the blood of family members and sexual partners of myeloma patients. sequencing of hhv-8 open-reading frames (orfs) shows differences between ... | 1999 | 9989483 |
| role of cytokines in multiple myeloma. | recent research in the biology of multiple myeloma (mm) has produced new insight into the factors that control the growth and survival of myeloma cells. in particular, there is a greater appreciation of the pathogenic role of the cytokines, such as interleukin-6 (il-6) and il-1beta in the conversion of monoclonal gammopathy of undetermined significance (mgus) to mm and in the proliferation and survival of myeloma cells. infection of dendritic cells with kaposi's sarcoma-associated herpesvirus (k ... | 1999 | 9989484 |
| kaposi's sarcoma-associated herpesvirus encodes a bzip protein with homology to bzlf1 of epstein-barr virus. | kaposi's sarcoma-associated herpesvirus (kshv) is a recently discovered human gamma herpesvirus strongly implicated in aids-related neoplasms. we report here the identification in the kshv genome of a gene for a protein designated k-bzip and belonging to the basic-leucine zipper (bzip) family of transcription factors. k-bzip shows significant homology to bzlf1, which plays a key role in the replication and reactivation of epstein-barr virus. k-bzip is a homodimerizing protein of 237 amino acids ... | 1999 | 9971770 |
| kinetics of kaposi's sarcoma-associated herpesvirus gene expression. | herpesvirus gene expression can be classified into four distinct kinetic stages: latent, immediate early, early, and late. here we characterize the kinetic class of a group of 16 kaposi's sarcoma-associated herpesvirus (kshv)/human herpesvirus 8 genes in a cultured primary effusion cell line and examine the expression of a subset of these genes in ks biopsies. expression of two latent genes, lana and vflip, was constitutive and was not induced by chemicals that induce the lytic cycle in primary ... | 1999 | 9971806 |
| three distinct regions of the murine gammaherpesvirus 68 genome are transcriptionally active in latently infected mice. | the program(s) of gene expression operating during murine gammaherpesvirus 68 (gammahv68) latency is undefined, as is the relationship between gammahv68 latency and latency of primate gammaherpesviruses. we used a nested reverse transcriptase pcr strategy (sensitive to approximately one copy of gammahv68 genome for each genomic region tested) to screen for the presence of viral transcripts in latently infected mice. based on the positions of known latency-associated genes in other gammaherpesvir ... | 1999 | 9971815 |
| induction of human herpesvirus-8 dna replication and transcription by butyrate and tpa in bcbl-1 cells. | human herpesvirus-8 (hhv-8) is a gammaherpesvirus that is present primarily in a state of low level persistence in primary effusion lymphoma cell lines. using bcbl-1 cells that harbour hhv-8 but lack epstein-barr virus, we demonstrate that sodium butyrate is much more effective than the phorbol ester 12-o-tetradecanoyl phorbol-13-acetate (tpa) at inducing high levels of class ii and iii virus transcription and viral dna replication, but also initiates apoptosis. apoptosis occurs prior to assembl ... | 1999 | 9934688 |
| classic kaposi's sarcoma as a second primary neoplasm. | although the incidence of classic kaposi's sarcoma (cks) has been investigated, its occurrence following a primary neoplasm and its association with this first neoplasm need to be determined. we analyzed a series of 124 patients with a secondary cks (8.4% of a total of 1485 incident cases) which occurred between 1961 and 1992 in the jewish israeli population. data on first neoplasms and subsequent kaposi's sarcoma were retrieved from the israel cancer registry. acquired-immune-deficiency-syndrom ... | 1999 | 9935196 |
| five cases of kaposi's sarcoma in kidney graft recipients: possible influence of the immunosuppressive therapy. | kaposi's sarcoma (ks) is an human herpesvirus 8-associated tumor, occurring in immunocompromised patients. we report here an increased incidence of ks among kidney graft recipients (kgrs) during the last 2 years, concomitant to the introduction of the immunosuppressant mycophenolate mofetil (mmf). | 1999 | 9921817 |
| human herpes-virus 8 seropositive patient with skin and graft kaposi's sarcoma after lung transplantation. | kaposi's sarcoma (ks) has been reported after solid organ transplantation mostly in recipients of renal, liver, heart, and bone allografts. we describe the first case of a patient with lung transplantation who developed ks of the skin, but also of the lung graft. the tumors were localized to places of previous trauma, implying the involvement of a koebner phenomenon. moreover, a polymerase chain reaction assay revealed the presence of dna sequences of herpesvirus 8 (hhv-8) on tissue of the cutan ... | 1999 | 10025855 |
| absence of kaposi's sarcoma--associated herpesvirus dna sequences in multiple myeloma. | 1999 | 10025982 | |
| pericardial involvement in human immunodeficiency virus infection. | previous studies have showed that the pericardium is frequently involved in hiv infection. however, the characteristics and etiology of the pericardial abnormalities that have been found remained poorly defined. we analyzed the features of pericardial involvement in these patients and investigated the clinical variables associated with moderate and severe effusions. | 1999 | 10027441 |
| detection of kaposi's sarcoma herpesvirus dna sequences in multiple myeloma bone marrow stromal cells. | whether kaposi's sarcoma herpesvirus (kshv) is associated with multiple myeloma (mm) remains controversial. we assayed for kshv dna sequences in long-term bone marrow stromal cells (bmscs) from 26 patients with mm and 4 normal donors. polymerase chain reaction (pcr) using primers which amplify a kshv gene sequence to yield a 233-bp fragment (ks330233 within open reading frame 26) was negative in all cases. aliquots of these pcr products were used as templates in subsequent nested pcr, with prime ... | 1999 | 10029574 |
| bone marrow and peripheral blood dendritic cells from patients with multiple myeloma are phenotypically and functionally normal despite the detection of kaposi's sarcoma herpesvirus gene sequences. | multiple myeloma (mm) cells express idiotypic proteins and other tumor-associated antigens which make them ideal targets for novel immunotherapeutic approaches. however, recent reports show the presence of kaposi's sarcoma herpesvirus (kshv) gene sequences in bone marrow dendritic cells (bmdcs) in mm, raising concerns regarding their antigen-presenting cell (apc) function. in the present study, we sought to identify the ideal source of dcs from mm patients for use in vaccination approaches. we c ... | 1999 | 10029575 |
| human immunodeficiency virus-associated pericardial effusion: report of 40 cases and review of the literature. | human immunodeficiency virus (hiv)-associated pericardial effusion is common. we present its clinical features, cause, and prognosis on the basis of a review of 40 cases at a single public hospital. | 1999 | 10047635 |
| human serum antibodies to a major defined epitope of human herpesvirus 8 small viral capsid antigen. | the major antibody-reactive epitope of the small viral capsid antigen (svca) of human herpesvirus 8 (hhv-8) was defined by use of overlapping peptides. strong igg reactivity was found among approximately 50% of 44 human immunodeficiency virus-positive or -negative patients with kaposi's sarcoma and 13 subjects who were seropositive by immunofluorescence assay (ifa) for the latent hhv-8 nuclear antigen. only 1 of 106 subjects seronegative for both lytic and latent hhv-8 antigens and 10 of 81 subj ... | 1999 | 10068601 |
| high seroprevalence of antibodies to human herpesvirus-8 in egyptian children: evidence of nonsexual transmission. | in western countries, human herpesvirus-8 (hhv-8) appears to be transmitted mainly by sexual contact. to evaluate the role of other transmission routes, especially in developing countries, we estimated the seroprevalence of hhv-8 in egyptian children, who, if seropositive, would have acquired the virus through a nonsexual route. | 1999 | 10070947 |
| absence of kaposi's sarcoma-associated virus (human herpesvirus-8) sequences in angiosarcoma. | kaposi's sarcoma-associated virus (human herpesvirus-8 [hhv8]) sequences have been identified in both aids-associated and aids-non-associated kaposi's sarcoma, but data relating to the detection of hhv8 sequences in angiosarcoma are variable. one study showed hhv8 sequences in 29% (7/24) of angiosarcomas, but others have not confirmed these results. we evaluated 33 angiosarcomas for hhv8 sequences to determine the frequency of this virus in angiosarcomas and its possible pathogenetic significanc ... | 1999 | 10071235 |
| immunodeficiency-associated lymphoproliferative disorders. | the incidence of lymphoproliferative disease is significantly higher in individuals who have congenital, acquired, or iatrogenically induced immunodeficiency. the immunodeficiency-associated lymphoproliferative disorders are clinically and pathologically heterogeneous, are of variable clonal composition, and vary according to the immunodeficiency syndrome. nonetheless, they share several features, including frequent origination in or involvement of extranodal sites, diffuse aggressive histology, ... | 1999 | 10071343 |
| human herpesvirus-8 (hhv-8) associated with small non-cleaved cell lymphoma in a child with aids. | the association of human herpesvirus-8 (hhv-8) with a small non-cleaved cell lymphoma is described in a child with the acquired immunodeficiency syndrome (aids) who developed a malignant pleural effusion and radiologic evidence of multiple solid tumors. hhv-8 dna and epstein-barr virus dna were identified in pleural fluid cells by polymerase chain reaction (pcr) amplification. the serum antibody titer against lytic hhv-8 proteins was 1:640; antibodies to latent hhv-8 proteins were not detected. ... | 1999 | 10072113 |
| activation in vivo of retroperitoneal fibromatosis-associated herpesvirus, a simian homologue of human herpesvirus-8. | retroperitoneal fibromatosis-associated herpesvirus of rhesus macaques (rfhvmm) is a gammaherpesvirus closely related to human herpesvirus-8 (hhv-8), which is thought to be a necessary cofactor for the development of kaposi's sarcoma (ks) in humans. here, rfhvmm infection of rhesus macaques exposed to the d-type retrovirus simian retrovirus-2 (srv-2) is described. development of srv-2 viraemia, infection with simian immunodeficiency virus or administration of cyclosporin a could result in persis ... | 1999 | 10073709 |
| sequence and genomic analysis of a rhesus macaque rhadinovirus with similarity to kaposi's sarcoma-associated herpesvirus/human herpesvirus 8. | we have sequenced the long unique region (lur) and characterized the terminal repeats of the genome of a rhesus rhadinovirus (rrv), strain 17577. the lur as sequenced is 131,364 bp in length, with a g+c content of 52.2% and a cpg ratio of 1.11. the genome codes for 79 open reading frames (orfs), with 67 of these orfs similar to genes found in both kaposi's sarcoma-associated herpesvirus (kshv) (formal name, human herpesvirus 8) and herpesvirus saimiri. eight of the 12 unique genes show similarit ... | 1999 | 10074154 |
| human herpesvirus 8 seroprevalence and evaluation of nonsexual transmission routes by detection of dna in clinical specimens from human immunodeficiency virus-seronegative patients from central and southern italy, with and without kaposi's sarcoma. | in order to investigate the seroprevalence of human herpesvirus 8 (hhv-8) infection in central and southern italy, sera from human immunodeficiency virus (hiv)-seronegative subjects, with and without kaposi's sarcoma (ks), were analyzed by immunofluorescence assay, using bc-3, a cell line latently infected with hhv-8. high titers of antibody against hhv-8 lytic and latent antigens were detected in all 50 ks patients studied, while in 50 hiv-seronegative subjects without ks, 32 (64%) were found p ... | 1999 | 10074540 |
| breast cancer and hiv: what do we know? | the present acquired human immunodeficiency syndrome-defining neoplasms are kaposi's sarcoma, non-hodgkins lymphoma, and cervical cancer. however, other malignancies have recently been associated with human immunodeficiency virus (hiv) infection. is there also a link between breast cancer and hiv infection? breast cancer seems to be more aggressive in the setting of immunocompromise by hiv infection, as demonstrated by the clinical course of two patients recently treated at this institution and ... | 1999 | 10075292 |
| human herpesvirus 8 in hematologic diseases. | human herpesvirus type 8 (hhv-8), also known as kaposi's sarcoma-associated herpesvirus (kshv) is a new member of the g-herpesvirus family. it is an unusual herpesvirus in that it carries a large number of genes that encode oncoproteins or cell signaling proteins. in addition to being the causative agent of both hiv-associated and non-hiv-associated kaposi's sarcoma this dna tumor virus has been implicated in the pathogenesis of several diseases. these include multiple myeloma (mm), waldenstöm's ... | 1999 | 10079383 |
| kaposi's sarcoma in women: a clinicopathologic study. | although all epidemiologic subsets of kaposi's sarcoma (ks) (i.e., sporadic, endemic, epidemic, and iatrogenic) have an association with human herpes virus 8 (hhv8), these subsets occur in patient populations with distinctive clinical features. to a variable degree men outnumber women in all subsets. | 1999 | 10082593 |
| transplantation-associated kaposi's sarcoma: herpesvirus 8 transmission through renal allografts. | 1999 | 10083406 | |
| herpesvirus 8 inclusions in primary effusion lymphoma: report of a unique case with t-cell phenotype. | we describe a case of primary effusion lymphoma with t-cell phenotype, mixed genotype, and intranuclear herpesvirus inclusions visible with the light microscope. cells were studied by immunohistochemical analysis, in situ hybridization, immunoglobulin and t-cell receptor gene rearrangement, and polymerase chain reaction. primary effusion lymphoma cells with t-cell phenotype revealed herpesvirus 8 inclusions predominantly seen in apoptotic cells, suggesting that productive viral infection is asso ... | 1999 | 10086517 |
| kaposi's sarcoma: breeding ground of herpesviridae - a tour de force over viral evolution (review). | after reviewing the molecular biological basis of prominent theories for the integration of viruses into the earliest forms of living matter, an account is given on the immunoevasive strategies viruses have had to acquire in order to secure their existence against the most sophisticated anti-viral defensive mechanisms evolving in their hosts. herpes-viridae and kaposi's sarcoma illustrate the complexity of host-virus relationship. in following the evolutionary steps of simians and hominoids to h ... | 1999 | 10087309 |
| [hhv8 infection (kaposi's sarcoma)]. | 1999 | 10088335 | |
| activation of cyclin a gene expression by the cyclin encoded by human herpesvirus-8. | human herpesvirus-8 (hhv-8), also known as kaposi's sarcoma-associated herpesvirus, encodes a protein, referred to as hhv8-vcyc, with sequence similarity to human g1 cyclins, in particular of the d type. hhv8-vcyc is expressed in kaposi's sarcoma and functional analysis suggests that it can activate cyclin-dependent kinases (cdk) and thereby trigger inactivation of the retinoblastoma protein (prb), indicating that hhv8-vcyc may contribute to the oncogenic potential of hhv-8. we show here that hh ... | 1999 | 10091992 |
| kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) encodes a homologue of the epstein-barr virus bzip protein eb1. | analysis of the recently completed genomic sequence of kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) revealed that orf 50 encodes a protein with homology to the epstein-barr virus (ebv) transcription factor r. in this report, we show that orf k8, contiguous to orf 50, is interrupted by two introns and that the spliced rna is translated into a bzip protein that has homology to the ebv transcription factor eb1. the newly characterized k8 protein forms homodimers but does not hetero ... | 1999 | 10091993 |
| ebv gene expression not altered in rheumatoid synovia despite the presence of ebv antigen-specific t cell clones. | t cells infiltrating the rheumatoid arthritis (ra) joint are oligoclonal, implicating an ag-driven process, but the putative joint-specific ags remain elusive. here we examine expression of selected ebv genes in ra synovia and find no abnormal expression in ra. dna of cmv and ebv was detectable by pcr in the synovial tissue of ra. rna of several latent and lytic ebv genes was also detectable. however, there were no differences in ebv gene expression in synovial tissues or peripheral blood when c ... | 1999 | 10092832 |
| efficient persistence of extrachromosomal kshv dna mediated by latency-associated nuclear antigen. | primary effusion lymphoma (pel) cells harbor kaposi's sarcoma-associated herpesvirus (kshv) episomes and express a kshv-encoded latency-associated nuclear antigen (lana). in pel cells, lana and kshv dna colocalized in dots in interphase nuclei and along mitotic chromosomes. in the absence of kshv dna, lana was diffusely distributed in the nucleus or on mitotic chromosomes. in lymphoblasts, lana was necessary and sufficient for the persistence of episomes containing a specific kshv dna fragment. ... | 1999 | 10213686 |
| constitutive cytokine production by primary effusion (body cavity-based) lymphoma-derived cell lines. | primary effusion lymphoma (pel) is a new lymphoma entity occurring predominantly, but not exclusively in hiv+ patients with acquired immunodeficiency syndrome (aids). pel grows exclusively in body cavities as serous lymphomatous effusion without evidence of mass disease or dissemination. the cells are infected with the newly discovered human herpesvirus-8 (hhv-8), often accompanied by co-infection with epstein-barr virus (ebv). several lymphoma cell lines have been established from patients with ... | 1999 | 10214873 |
| absence of human herpesvirus-8 dna in angiosarcoma. | 1999 | 10215793 | |
| epidemiology of kaposi's sarcoma-associated herpesvirus/human herpesvirus 8. | 1999 | 10218100 | |
| pleural fluid findings in patients with the acquired immunodeficiency syndrome: correlation with concomitant pulmonary disease. | we sought to define the spectrum of conditions associated with pleural effusion (pe) in patients with the acquired immunodeficiency syndrome (aids) who submitted to diagnostic thoracentesis. | 1999 | 10219358 |
| hhv-8/kshv is not associated with aids-related primary central nervous system lymphoma. | primary central nervous system lymphoma (pcnsl) is a major complication of the late stages of human immunodeficiency virus (hiv) disease. epstein barr virus (ebv) infection is the only genetic lesion consistently associated with this neoplasia. recently, it has been proposed that the pathogenesis of aids-related pcnsl (aids-pcnsl) may be associated with infection by human herpesvirus-8/kaposi's sarcoma associated herpesvirus (hhv-8/kshv), although at present such association remains controversia ... | 1999 | 10219737 |
| human herpesvirus 8 primary infection occurs during childhood in cameroon, central africa. | while in the united states and northern europe, human herpesvirus 8 (hhv-8) appears to be mainly sexually transmitted with primary infection occurring in adulthood, the modes of transmission remain unknown in east and central africa, where kaposi's sarcoma (ks) is a long-standing endemic disease, occurring not only in adults but also in children. the aim of our present study was to determine the prevalence of hhv-8 infection in children from yaounde, cameroon, central africa. specific antibodies ... | 1999 | 10188717 |
| risk factors for rebleeding and mortality from acute upper gastrointestinal hemorrhage in human immunodeficiency virus infection. | in the general population, acute upper gastrointestinal hemorrhage (ugih) is a common problem that results in significant morbidity and mortality. the aim of this study was to determine the etiology, clinical outcome, and risk factors for rebleeding and mortality in a large cohort of human immunodeficiency virus (hiv)-infected patients with acute ugih. | 1999 | 10022629 |
| detection of kaposi's sarcoma-associated herpesvirus in peripheral blood cells in human immunodeficiency virus infection: association with kaposi's sarcoma, cd4 cell count, and hiv rna levels. | kaposi's sarcoma-associated herpesvirus (kshv) dna, consistently found in kaposi's sarcoma (ks) tissues, was sought in peripheral blood mononuclear cells (pbmcs) of hiv-infected individuals. to determine quantitative relationships between the presence of kshv dna in pbmcs, cd4 cell counts, plasma hiv rna levels, and the development of ks, we designed a cross-sectional study of prospectively collected pbmc samples from ongoing cohort studies. pbmcs were collected from 142 hiv-seropositive individ ... | 1999 | 10024052 |
| human herpesvirus 8 and renal transplantation. | 1999 | 10189287 | |
| human herpesvirus 8 and renal transplantation. | 1999 | 10189288 | |
| a complex immunodeficiency. idiopathic cd4+ t-lymphocytopenia and hypogammaglobulinemia associated with hhv8 infection, kaposi's sarcoma and gastric cancer. | 1999 | 10190958 | |
| comparison of human sera reactivities in immunoblots with recombinant human herpesvirus (hhv)-8 proteins associated with the latent (orf73) and lytic (orfs 65, k8.1a, and k8.1b) replicative cycles and in immunofluorescence assays with hhv-8-infected bcbl-1 cells. | the development of reliable, sensitive, and specific serological methods for the detection of human herpesvirus-8 (hhv-8) antibodies is critical for a thorough understanding of hhv-8 prevalence and pathogenesis. to evaluate the potential usefulness of hhv-8 proteins in measuring the responses against both latent and lytic antigens, we selected 1 latent [open reading frame (orf) 73] antigen and 3 hhv-8 lytic antigens (orfs 65, k8.1a, and k8.1b) previously identified as immunogenic [virology (1998 ... | 1999 | 10191203 |
| human herpesvirus 8 infection, castleman's disease and poems syndrome. | 1999 | 10192466 | |
| hhv-8 and multiple myeloma. | recently, a new member of the gamma-herpesvirus family, human herpesvirus 8 (hhv-8), was identified in a case of kaposi's sarcoma. this virus has also been found in the nonmalignant dendritic cells of the bone marrow from myeloma patients. in addition, hhv-8 is also detectable in the peripheral blood of most patients although its absence suggests earlier stage disease. by contrast, this virus is undetectable in the blood of family members and sexual partners of myeloma patients. sequencing of hh ... | 1999 | 10192878 |
| human immunodeficiency virus negative kaposi sarcoma and lymphoproliferative disorders. | the concomitant occurrence of more than one primary neoplasm in the same individual has led researchers to seek possible common etiopathogenetic factors. kaposi sarcoma (ks) is a multicentric neoplasm of vascular origin and perhaps viral etiology. four forms of ks are known: classic or mediterranean, endemic or african, posttransplant, and epidemic or acquired immunodeficiency syndrome-associated ks. in its classic form ks mainly affects elderly people and often has a long and indolent course th ... | 1999 | 10193954 |
| antibodies to human herpesvirus 8 in poems (polyneuropathy, organomegaly, endocrinopathy, m protein, skin changes) syndrome with multicentric castleman's disease. | 1999 | 10194095 | |
| alpha interferon inhibits human herpesvirus 8 (hhv-8) reactivation in primary effusion lymphoma cells and reduces hhv-8 load in cultured peripheral blood mononuclear cells. | infection by human herpesvirus 8 (hhv-8) is associated with the development of kaposi's sarcoma (ks). since regression of ks can be achieved by treatment of the patients with alpha interferon (ifn-alpha), we analyzed the effects of ifn-alpha or anti-ifn-alpha antibodies (ab) on hhv-8 latently infected primary effusion lymphoma-derived cell lines (bcbl-1 and bc-1) and on peripheral blood mononuclear cells (pbmc) from patients with all forms of ks and from at-risk subjects. ifn-alpha inhibited in ... | 1999 | 10196299 |
| high-level variability in the orf-k1 membrane protein gene at the left end of the kaposi's sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. | infection with kaposi's sarcoma (ks)-associated herpesvirus (kshv) or human herpesvirus 8 (hhv8) is common in certain parts of africa, the middle east, and the mediterranean, but is rare elsewhere, except in aids patients. nevertheless, hhv8 dna is found consistently in nearly all classical, endemic, transplant and aids-associated ks lesions as well as in some rare aids-associated lymphomas. the concept that hhv8 genomes fall into several distinct subgroups has been confirmed and refined by pcr ... | 1999 | 10196312 |
| cellular tropism and viral interleukin-6 expression distinguish human herpesvirus 8 involvement in kaposi's sarcoma, primary effusion lymphoma, and multicentric castleman's disease. | human herpesvirus 8 (hhv-8) infection has been implicated in the etiology of kaposi's sarcoma (ks), primary effusion lymphoma (pel), and multicentric castleman's disease (mcd), three diseases that frequently develop in immunocompromised, human immunodeficiency virus-positive individuals. one hypothesis that would account for different pathological manifestations of infection by the same virus is that viral genes are differentially expressed in heterogeneous cell types. to test this hypothesis, w ... | 1999 | 10196314 |
| epstein-barr virus-associated intravascular lymphomatosis within kaposi's sarcoma in an aids patient. | intravascular lymphomatosis (il) is an unusual neoplasm characterized by multifocal proliferation of lymphoma cells exclusively within the blood vessels. we report here a patient with acquired immunodeficiency syndrome (aids) and disseminated kaposi's sarcoma. a 233-bp amplification product of hhv-8 was detected in the dna extracted from specimens of kaposi's sarcoma at different sites by polymerase chain reaction (pcr). at autopsy, the vessels within the kaposi's sarcoma were dilated and filled ... | 1999 | 10199480 |
| distribution of human herpesvirus-8 latently infected cells in kaposi's sarcoma, multicentric castleman's disease, and primary effusion lymphoma. | human herpesvirus 8 (hhv-8, also called kshv) is linked to the etiopathogenesis of kaposi's sarcoma (ks), multicentric castleman's disease (mcd), and primary effusion lymphoma (pel). the universal presence of hhv-8 in early ks has not yet been shown. we used a mab (ln53) against latent nuclear antigen-1 (lna-1) of hhv-8 encoded by orf73 to study the distribution of the cell types latently infected by hhv-8 in patch, plaque, and nodular ks, mcd, and pel. in early ks, hhv-8 is present in <10% of c ... | 1999 | 10200299 |
| unique properties of a second human herpesvirus 8-encoded interferon regulatory factor (virf-2). | human herpesvirus 8/kaposi's sarcoma herpesvirus (hhv-8/kshv) contains, in addition to genes required for viral replication, an unique set of nonstructural genes which may be part of viral mimicry and contribute to viral replication and pathogenesis in vivo. among these, hhv-8 encodes four open reading frames (orfs) that show homology to the transcription factors of the interferon regulatory factor (irf) family. in this study we demonstrate that one of these orfs (virf-2) encodes a protein with ... | 1999 | 10200596 |
| cellular localization of human herpesvirus 8 in nonneoplastic lymphadenopathies and chronic interstitial pneumonitis by in situ polymerase chain reaction studies. | to study the cellular localization of human herpesvirus 8 (hhv-8) in rare cases of hhv-8 infection from italy that are associated neither with human immunodeficiency virus (hiv) infection nor kaposi's sarcoma (ks). | 1999 | 10200598 |
| multicenter comparison of pcr assays for detection of human herpesvirus 8 dna in semen. | reported prevalences of human herpesvirus 8 (hhv-8) (kaposi's sarcoma-associated herpesvirus) in semen have ranged widely. this is possibly due to differences in assay sensitivity, geographic or population-based differences in the true presence of the virus in semen, and pcr contamination. this study assessed interlaboratory sensitivity and reproducibility in the analysis of blinded experimental panels, each consisting of 48 specimens and being composed of semen specimens from different healthy ... | 1999 | 10203474 |
| human herpesvirus 8 (hhv-8) in familial kaposi's sarcoma. | human herpesvirus 8 (hhv-8) has been detected in various epidemiological forms of kaposi's sarcoma (ks). since familial ks cases are exceedingly rare and the occurrence of familial ks in siblings has thought to depend rather on genetic factors than on a viral factor, familial ks has not been investigated for the presence of hhv-8. to investigate whether hhv-8 is present also in this rare form of ks, we examined tumor biopsies of 2 siblings with familial ks for the presence of hhv-8 specific dna ... | 1999 | 10206723 |
| involvement of human interleukin-6 in systemic manifestations of human herpesvirus type 8-associated multicentric castleman's disease. | 1999 | 10207568 | |
| cd4+ t-lymphocytopenia, kaposi's sarcoma, hhv-8 infection, severe seborrheic dermatitis, and onychomycosis in a homosexual man without hiv infection. | 1999 | 10208627 | |
| transcriptional analysis of human herpesvirus-8 open reading frames 71, 72, 73, k14, and 74 in a primary effusion lymphoma cell line. | we examined the transcription and splicing of open reading frames (orfs) 71 (k13)-74 of human herpesvirus-8 (hhv-8) in the primary effusion lymphoma cell line bcp-1 (latently infected with hhv-8), using a combination of northern blot analysis, rt-pcr, and rapid amplification of cdna ends (pcr-race). the three genes encoded by orfs 71, 72, and 73 [viral flice inhibitory protein (v-flip), v-cyclin, latent nuclear antigen (lna)] are transcribed from a common transcription start site in bcp-1 cells ... | 1999 | 10208923 |
| the human herpesvirus-type 8 is not involved in malignant melanoma. | malignant melanomas were supposed to harbour the human herpesvirus-type 8 (hhv-8) genome, as melanoma cells were reported to express interleukin-6 and a homologue of interleukin-6 was found in the hhv-8 genome. we therefore investigated 33 primary malignant melanomas by polymerase chain reaction, but could not find this tumorigenic gamma-herpesvirus in any tumour. | 1999 | 10389979 |
| cancer (or lack thereof) and viruses. | 1999 | 10390633 | |
| primary effusional lymphoma: a new non-hodgkin s lymphoma entity. | a distinct non-hodgkin s lymphoma (nhl) entity that grow in the body cavities as lymphomatous effusions in the absence of clinically identifiable tumor masses has been defined as primary effusional lymphoma (pel). this lymphoma characterized by distinetive morphology, immunophenotype, genotype and association with kaposi s sarcoma-associated herpesvirus (khsv)/human herpesvirus-8 (hhv-8) infection. in this minireview, the clinico-pathological and biological characteristics of pels are summarized ... | 1999 | 10393357 |
| high expression of hhv-8-encoded orf73 protein in spindle-shaped cells of kaposi's sarcoma. | human herpesvirus 8 (hhv-8) has been demonstrated previously in kaposi's sarcoma (ks) tissues by immunohistochemistry, in situ polymerase chain reaction, and in situ hybridization. the hhv-8-encoded protein orf73 is a 222- or 234-kd protein named latent nuclear antigen (lna) or latency-associated nuclear antigen (lana) that is identified in hhv-8-infected cell lines by immunofluorescence assay. in the present study, a rabbit antibody against a recombinant orf73 protein was developed. immunofluor ... | 1999 | 10393835 |
| the role of human herpesvirus-8, (hhv-8), in multiple myeloma pathogenesis. | 1999 | 10396081 | |
| mucocutaneous manifestations of hiv infection: a retrospective analysis of 145 cases in a chinese population in malaysia. | mucocutaneous lesions directly related to human immunodeficiency virus (hiv) infection usually present as initial manifestations of immune deficiency. the most common mucocutaneous lesions are kaposi's sarcoma, histoplasmosis, oro-esophageal candidiasis, oral hairy leukoplakia, and, in asia, penicillium marneffei infection. non-hiv-related skin lesions, such as psoriasis, seborrheic dermatitis, and nodular prurigo, may be the initial presentation among hiv infected patients attending outpatient ... | 1999 | 10397587 |
| the tat protein of human immunodeficiency virus type-1 promotes vascular cell growth and locomotion by engaging the alpha5beta1 and alphavbeta3 integrins and by mobilizing sequestered basic fibroblast growth factor. | the tat protein of human immunodeficiency virus type-1 (hiv-1) has been shown to be released during acute infection of t cells by hiv-1 and to promote angiogenesis and kaposi's sarcoma (ks) development in infected individuals. in this study, we investigated the molecular mechanisms responsible for the angiogenic effects of tat. the results shown herein indicate that two different tat domains cooperate to induce these effects by different pathways. the arginine-glycine-aspartic acid (rgd) sequenc ... | 1999 | 10397733 |
| dominant human herpesvirus type 8 rna transcripts in classical and aids-related kaposi's sarcoma. | skin biopsy sections of kaposi's sarcoma (ks) from 25 patients (5 aids-related, 20 classical cases) were histologically staged and hybridized in situ with oligonucleotide probes for constitutively transcribed human herpesvirus 8 (hhv-8) mrna t0.7 and t1.1 using a colourimetric technique. t1.1 increases during experimental induction of the viral lytic phase in the hhv-8-infected lymphocytes of primary effusion lymphoma and its colourimetric detection in ks cells presumably corresponds to virion p ... | 1999 | 10398125 |
| [infection and angiomatous cutaneous lesions]. | the occurrence of angiomatous cutaneous lesions in the presence of an infective process is not a frequent phenomenon. most infectious diseases are associated with an exanthematous reaction. the combination of an infective illness and angiomatous lesions is seen essentially in the bartonelloses and in kaposi's disease. bartonelloses: group of infections due to alpha-proteobacteria such as bartonella. bartonella bacilliformis (bb), is the causal agent of carrion's disease, the chronic cutaneous fo ... | 1999 | 10399647 |
| immunoglobulin v region gene use and structure suggest antigen selection in aids-related primary effusion lymphomas. | primary effusion lymphoma (pel) is a lymphoproliferation of b cells infected by kaposi's sarcoma-associated herpes-virus/human herpesvirus-8 and reflecting a late stage of b cell differentiation close to plasma cell. apart from viral infection, the pathogenesis of pel is currently unclear. the aim of the present study was to investigate the role of antigen stimulation and selection in the evolution of pel. in order to assess the specific variable heavy (vh) and light (vl) genes used by pel and t ... | 1999 | 10400426 |
| comparison of genetic variability at multiple loci across the genomes of the major subtypes of kaposi's sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame k15 alleles at the right-hand end. | kaposi's sarcoma (ks)-associated herpesvirus or human herpesvirus 8 (hhv8) dna is found consistently in nearly all classical, endemic, transplant, and aids-associated ks lesions, as well as in several aids-associated lymphomas. we have previously sequenced the genes for the highly variable open reading frame k1 (orf-k1) protein from more than 60 different hhv8 samples and demonstrated that they display up to 30% amino acid variability and cluster into four very distinct evolutionary subgroups (t ... | 1999 | 10400762 |
| long-term infection and transformation of dermal microvascular endothelial cells by human herpesvirus 8. | human herpesvirus 8 (hhv8) infects kaposi's sarcoma (ks) spindle cells in situ, as well as the lesional endothelial cells considered to be spindle cell precursors. the hhv8 genome contains several oncogenes, suggesting that infection of endothelial and spindle cells could induce cellular transformation and tumorigenesis and promote the formation of ks lesions. to investigate the potential of hhv8 infection of endothelial cells to contribute to the development of ks, we have developed an in vitro ... | 1999 | 10400787 |
| identification of a spliced gene from kaposi's sarcoma-associated herpesvirus encoding a protein with similarities to latent membrane proteins 1 and 2a of epstein-barr virus. | kaposi's sarcoma-associated herpesvirus (kshv) or human herpesvirus 8 (hhv-8) is a novel herpesvirus implicated as the causative agent of kaposi's sarcoma (ks), primary effusion lymphoma, and some cases of multicentric castleman's disease. kshv persists in the majority of ks spindle (endothelial tumor) cells and lymphoid cells in a latent form, with only a limited set of viral genes expressed in a tissue-specific manner. here, we report the identification of a family of alternatively-spliced tra ... | 1999 | 10400794 |
| comparison of the immunoglobulin-g-specific seroreactivity of different recombinant antigens of the human herpesvirus 8. | the open reading frames orf 52, orf 65, k12, and k8.1 of the human herpesvirus 8 (hhv8) were expressed as glutathione-s-transferase (gst) fusion proteins and analysed by western blotting (wb) and enzyme-linked immunosorbent assay (elisa). the open reading frame (orf) 65 and k8.1 antigens gave the highest reactivity (71%) in sera from hiv-dependent kaposi's sarcoma (ks) patients. therefore both antigens appear to be essential for hhv8 diagnostics, whereas orf k12 and orf 52 were of minor importan ... | 1999 | 10405355 |
| enhancing the response to interferon-alpha. | though initially recognized as antiviral agents, it was soon demonstrated that certain neoplasms were particularly sensitive to interferon-alpha (ifn-alpha). indeed, the initial success of systemic ifn-alpha treatment in aids-associated kaposi's sarcoma (aids-ks) occurred before identification of the human immunodeficiency virus (hiv) and in the absence of any coherent view of ks pathogenesis. with a more comprehensive understanding how ks develops and which circumstances provide an increased vi ... | 1999 | 10405886 |
| prevalence of infection with human herpesvirus 8/kaposi's sarcoma herpesvirus in rural south africa. | to determine prevalence of infection with human herpesvirus 8 (hhv-8)/kaposi's sarcoma herpesvirus (kshv) and to gain some insight into possible transmission dynamics of this novel virus in south africa. | 1999 | 10416461 |
| variability and evolution of kaposi's sarcoma-associated herpesvirus in europe and africa. international collaborative group. | to study the evolution of kaposi's sarcoma-associated herpesvirus (kshv) or human herpesvirus type 8 in europe and africa. | 1999 | 10416519 |
| [epidemiology of non-hodgkin's lymphoma: recent data]. | non-hodgkin's lymphoma consists of a group of disorders which is increasing for the last decades. in the usa, since the early 1970s, the incidence rate has increased by almost 60% and ranges from 3 to 4% per year, which is faster than for the majority of cancers. important advances in our knowledge of histopathology together with new diagnostic tools, as well as the improvement of the registered data and ageing of the population, only partially explain this increase. two viruses have been identi ... | 1999 | 10417426 |
| human herpesvirus type 8 and epstein-barr virus-associated cutaneous lymphoma taking anaplastic large cell morphology in a man with hiv infection. | human herpesvirus type 8 (hhv-8, kaposi's sarcoma-associated herpesvirus)-positive lymphoma taking anaplastic large cell morphology in the skin is described in a 46-year-old man with aids. multiple erythematous nodules appeared on the trunk and extremities during the treatment of aids. histological examination of cutaneous nodules showed dense infiltration of cd30 + atypical lymphoid cells in the deep dermis. immunoglobulin jh gene rearrangement was detected in these lymphoma cells. both epstein ... | 1999 | 10417531 |
| absence of a link between human herpesvirus 8 and pemphigus. | 1999 | 10417537 | |
| [ifn-tau, a new interferon type i with antiretroviral activity]. | type i interferon (ifn) such as ifn-alpha have demonstrated relative efficiency in hiv-infected patients with kaposi's sarcoma. nevertheless, their clinical uses have been restricted by several major side effects. ifn-tau is a non-cytotoxic type i ifn. in the present manuscript, we described its in vitro effects towards hiv replication and its mode of action. ifn-tau is a potent antiviral molecule that interferes with an early step of hiv biological cycle. moreover, it induces il-6 synthesis by ... | 1999 | 10418039 |
| hhv8-encoded vmip-i selectively engages chemokine receptor ccr8. agonist and antagonist profiles of viral chemokines. | uncertainty regarding viral chemokine function is mirrored by an incomplete knowledge of host chemokine receptor usage by the virally encoded proteins. one such molecule is vmip-i, a c-c type chemokine of undefined function and binding specificity, encoded by the kaposi's sarcoma herpesvirus hhv-8. we report here that vmip-i binds to and induces cytosolic [ca(2+)] signals in human t cells selectively through ccr8, a cc chemokine receptor associated with th2 lymphocytes. furthermore, using a pane ... | 1999 | 10419462 |
| establishing and characterizing a cd30-positive cell line harboring hhv-8 from a primary effusion lymphoma. | primary effusion lymphoma (pel, or body-cavity-based lymphoma [bcbl]) is a new subtype of non-hodgkin's lymphoma in which tumor cells locate in the body cavity exclusively. pel/bcbl is widely accepted as one of the neoplastic complications of aids, associated mostly with human herpesvirus 8 (hhv-8/kaposi's sarcoma-associated herpesvirus [kshv]) and epstein-barr virus (ebv). we established and characterized a pel cell line named ty-1 from a 47-year-old patient with aids. ty-1 exhibits indetermina ... | 1999 | 10421407 |
| [hhv 6,7 and 8. recently discovered herpesviruses explain the etiology of well-known diseases]. | three new members of the family of human herpesviruses (hhvs) have been identified in less than a decade, hhv 67 and 8. hhv-6 and hhv-7, both infecting t-lymphocytes and phylogenetically related to cytomegalovirus, were identified as causative agents of exanthema subitum. in addition, hhv-6 has been reported to manifest central nervous system tropism and to be frequently detected in normal brain tissue, but has also been associated with febrile seizures. hhv-7 has been suggested to be involved i ... | 1999 | 10423975 |
| proceedings of the second national aids malignancy conference. bethesda, maryland, usa. april 6-8, 1998. | 1999 | 10430210 | |
| overview of the epidemiology of malignancy in immune deficiency. | immune-suppressed populations experience higher rates of cancer than expected. the most common malignancies are non-hodgkin's lymphoma (nhl) in those with hiv infection, in organ transplant recipients, and in those with primary immune deficiencies; kaposi's sarcoma (ks) in those with hiv infection; and nonmelanoma skin cancer (sc) in transplant patients. these cancers are associated with infection with the epstein-barr virus (ebv) in nhl, human herpesvirus type 8 (hhv-8) in ks, and the human pap ... | 1999 | 10430211 |
| impact of potent antiretroviral therapy on the incidence of kaposi's sarcoma and non-hodgkin's lymphomas among hiv-1-infected individuals. multicenter aids cohort study. | effective hiv-1 therapies may directly or indirectly impact the development of aids-associated malignancies. using data from the multicenter aids cohort study, a longitudinal cohort study of the natural history of hiv-1 infection among homosexual men, the incidence rates of kaposi's sarcoma (ks) and non-hodgkin's lymphoma (nhl) over calendar time were determined for the 1813 hiv-1-seropositive men enrolled in 1984 through 1985. poisson regression models were used to identify statistically signif ... | 1999 | 10430217 |
| consideration of host-viral interactions in the pathogenesis of kaposi's sarcoma. | human herpesvirus type 8 (hhv-8) is a recently identified gamma herpesvirus that is associated with and most likely the etiologic agent of kaposi's sarcoma (ks). both monocytes and b lymphocytes appear to serve as reservoirs for hhv-8 in asymptomatic individuals, whereas hhv-8 is present in both spindle cells and microvascular endothelial cells within ks lesions. this suggests that a necessary event for the development of ks is infection of endothelial cells with hhv-8. this paper proposes a mod ... | 1999 | 10430220 |
| hhv-8 and multistep tumorigenesis. | 1999 | 10431201 | |
| in vitro sensitivity of kaposi's sarcoma cells to various chemotherapeutic agents including acyclic nucleoside phosphonates. | the involvement of a viral agent in the pathogenesis of kaposi's sarcoma (ks) points to antiviral agents as possible therapeutic and/or prophylactic options in the management of the disease. in the present study we investigated the antiproliferative effects of various chemotherapeutic agents, including acyclic nucleoside phosphonates, on the growth of ks-derived cells. nested pcr amplification demonstrated that these cells do not contain human herpesvirus 8 (hhv-8) dna sequences. the cytotoxicit ... | 1999 | 10431612 |
| oncological complications of human immunodeficiency virus disease and hematologic consequences of their treatment. | despite advances in antiretroviral therapy and in the treatment and prevention of opportunistic infections, oncological and consequent hematologic complications of human immunodeficiency virus (hiv) infection continue to occur and are of significant clinical importance. virus-related tumors (e.g., kaposi's sarcoma, induced by human herpesvirus 8; non-hodgkin's lymphoma, linked to epstein-barr virus; and anogenital tumors, linked to human papillomavirus) are frequent in patients with hiv-induced ... | 1999 | 10433562 |
| a biophenotypic human herpesvirus 8--associated primary bowel lymphoma. | 1999 | 10435572 | |
| [human herpes virus 8 and kaposi's sarcoma]. | 1999 | 10436595 | |
| serologic evidence of human herpesvirus 8 transmission by homosexual but not heterosexual sex. | epidemiologic studies link kaposi's sarcoma with a sexually transmitted agent. human herpesvirus 8 (hhv-8) is likely to be that agent, but routes of transmission are poorly described. a seroepidemiologic study was conducted to determine whether hhv-8 is transmitted sexually between heterosexuals. sera from 2718 patients attending a sexually transmitted disease (std) clinic were tested for antibodies to hhv-8 and herpes simplex virus type 2 (hsv-2). information on sex partners in the previous 12 ... | 1999 | 10438345 |
| heterogeneity of viral il-6 expression in hhv-8-associated diseases. | in order to characterize the expression of the viral interleukin-6 (vil-6) homologue in various human herpesvirus 8 (hhv-8)-associated diseases, in situ hybridization and immunohistochemistry were applied to formalin-fixed specimens. these assays showed consistent expression of vil-6 in primary effusion lymphomas and in a case of human immunodeficiency virus (hiv)-associated lymphadenopathy with a castleman's disease-like appearance. in contrast, kaposi's sarcoma specimens showed marked differen ... | 1999 | 10438372 |
| ccr5 delta32 deletion and reduced risk of toxoplasmosis in persons infected with human immunodeficiency virus type 1. the seroco-hemoco-serogest study groups. | this study attempted to determine whether the ccr5 delta32 deletion affected progression to certain first aids-defining illnesses in human immunodeficiency virus type 1-infected patients enrolled in the french seroco/hemoco/serogest cohorts. toxoplasmosis onset as a first aids-defining illness was significantly delayed in 253 heterozygous patients, compared with 1404 wild type patients. the relative risk of toxoplasmosis associated with heterozygosity was 0. 39 (95% confidence interval, 0.16-0.9 ... | 1999 | 10438395 |
| functional analysis of human herpesvirus 8-encoded viral interferon regulatory factor 1 and its association with cellular interferon regulatory factors and p300. | human herpesvirus 8/kaposi sarcoma-associated virus (hhv-8/kshv) contains, in addition to genes required for viral replication, a unique set of nonstructural genes which may be part of viral mimicry and contribute to viral replication and pathogenesis in vivo. among these, hhv-8 encodes four open reading frames (orfs) that showed homology to the transcription factors of the interferon regulatory factor (irf) family. the orf k9, viral irf 1 (virf-1), has been cloned, and it was shown that, when o ... | 1999 | 10438822 |