Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| effects of an intensive 4-week summer camp on cystic fibrosis: pulmonary function, exercise tolerance, and nutrition. | cystic fibrosis (cf) patients prefer exercise to most other forms of therapy, although objective improvement remains controversial. israeli cf patients have attended a summer program in switzerland for many years with subjective improvement. however, cf camps worldwide have been cancelled recently, due to fears of cross-infection with resistant organisms. therefore, we evaluated the effect of attending the camp on pulmonary function, exercise tolerance, and nutritional state in cf patients. | 2002 | 11948041 |
| detecting bacterial colonization of implanted orthopaedic devices by ultrasonication. | glycocalyx-producing bacteria have been observed on orthopaedic devices that were removed for reasons other than infection. it has been suggested that the bacteria adhere to foreign surfaces within a biofilm and elude standard culture techniques. the authors adapted previously used ultrasonication protocols that disrupt the surface biofilm before culturing removed orthopaedic devices from patients without clinical evidence of infection. patients having revision total joint arthroplasty of the hi ... | 2002 | 12360004 |
| multiple substrate growth kinetics of leptothrix discophora sp-6. | the growth parameters of leptothrix discophora sp-6 were quantified on the basis of the steady-state concentrations and utilization rates of pyruvate, dissolved oxygen, and concentration of microorganisms in a chemostat operated at 25 degrees c, ph 7.2, and an agitation rate of 350 rpm. the results showed that the microbial growth was limited by both pyruvate and dissolved oxygen. a combined growth kinetics model using monod growth kinetics for pyruvate and tessier growth kinetics for oxygen sho ... | 2002 | 12363350 |
| broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. | complete genome sequences are now available for many bacterial species that lack sophisticated genetic tools. we describe the development of a broad-host-range cre-lox system that allows antibiotic marker recycling in a variety of gram-negative bacteria. this system consists of an allelic exchange vector bearing a kanamycin cassette flanked by loxp sites and a tetracycline-resistant incp plasmid that provides expression of the cre recombinase. we demonstrate this system by generating unmarked de ... | 2002 | 12449384 |
| construction and evaluation of plasmid vectors optimized for constitutive and regulated gene expression in burkholderia cepacia complex isolates. | genetic studies with burkholderia cepacia complex isolates are hampered by the limited availability of cloning vectors and by the inherent resistance of these isolates to the most common antibiotics used for genetic selection. also, some of the promoters widely employed for gene expression in escherichia coli are inefficient in b. cepacia. in this study, we have utilized the backbone of the vector pme6000, a derivative of the pbbr1 plasmid that was originally isolated from bordetella bronchisept ... | 2002 | 12450816 |
| isolation of bacterial strains that produce the endocrine disruptor, octylphenol diethoxylates, in paddy fields. | topsoil samples were collected from 36 different paddy fields in west japan. each soil sample was incubated with a basal salt-medium containing 0.2% oppeo. twelve samples possessed oppeo-degrading activity, from which twelve cultures of oppeo-degrading bacteria were isolated. the isolated bacteria grew on a medium containing 0.2% oppeo as the sole carbon source, and op2eo and op3eo were accumulated in the medium under aerobic conditions. op1eo and octylphenol, which have often been identified in ... | 2002 | 12400675 |
| effect of ph on cadmium toxicity, speciation, and accumulation during naphthalene biodegradation. | lowering ph of a microbiological medium from 7 to 4 decreased cadmium toxicity during naphthalene biodegradation by a burkholderia sp. cadmium speciation and cadmium accumulation in the system were studied to explain this effect. cadmium speciation was determined by direct measurement and by geochemical modeling. previous studies have implicated the monovalent hydroxylated cadmium (cdoh+) species in the effect of ph on cadmium toxicity. modeling analysis predicted cdoh+ formation only at very lo ... | 2002 | 12371483 |
| advances in burkholderia cepacia complex. | burkholderia cepacia is an important opportunistic pathogen in certain compromised hosts, particularly those with either cystic fibrosis (cf) or chronic granulomatous disease. the "family" of bacteria known as b. cepacia is highly heterogeneous and is composed of at least nine discrete species or genomovars, constituting the b. cepacia complex. bacteria from the b. cepacia complex are particularly virulent in susceptible hosts, often causing necrotising invasive infection and death. whereas the ... | 2002 | 12376059 |
| ammonium hydroxide hydrolysis: a valuable support in the maldi-tof mass spectrometry analysis of lipid a fatty acid distribution. | lipid a is the lipophilic moiety of lipopolysaccharides (lpss), the major components of the external membrane of almost all gram-negative bacteria. it is responsible for the toxicity of lps and has a heterogeneous structure composed of a bis-phosphorylated glucosamine disaccharide backbone that is acylated at the positions 2, 3 of the glcn i (proximal) and glcn ii (distal) residue with o- and n-linked 3-hydroxy fatty acids (primary substitution). these fatty acids are further acylated by means o ... | 2002 | 12454282 |
| preventing burkholderia cepacia complex infection in cystic fibrosis: is there a middle ground? | 2002 | 12378182 | |
| risk factors for burkholderia cepacia complex colonization and infection among patients with cystic fibrosis. | to determine risk factors for acquiring burkholderia cepacia complex among patients with cystic fibrosis (cf). | 2002 | 12378190 |
| interspecies communication between burkholderia cepacia and pseudomonas aeruginosa. | burkholderia cepacia and pseudomonas aeruginosa are opportunistic pathogens that commonly cause pulmonary infections in cystic fibrosis patients and occasionally co-infect patients' lungs. both organisms possess quorum-sensing systems dependent on n-acyl homoserine lactone (n-acyl-hsl). cross-feeding assays demonstrated that p. aeruginosa and b. cepacia were able to utilize heterologous n-acyl-hsl signaling molecules. the ability of quorum-sensing genes from one species to complement the respect ... | 2002 | 12381027 |
| strategies for pcr based detection of burkholderia pseudomallei dna in paraffin wax embedded tissues. | recently, several cases of melioidosis imported to europe have been reported. the diagnosis of the acute or chronic infection remains challenging. this report describes an optimised protocol for fast and reliable dna preparation for use in two different polymerase chain reaction (pcr) assays, namely: (1) a seminested pcr assay targeting a genus specific sequence of the ribosomal protein subunit 21 (rpsu) gene and (2) a nested pcr assay targeting the gene encoding the filament forming flagellin ( ... | 2002 | 12456780 |
| the burkholderia pseudomallei oxyr gene: expression analysis and mutant characterization. | burkholderia pseudomallei (bp) is the causative agent of the life-threatening melioidosis in humans. the global transcription factor oxyr gene was isolated and characterized. it is located between recg, encoding a putative dna helicase, and katg, encoding a putative catalase-peroxidase. oxyr is expressed as a monocistronic 1 kb mrna and is induced by oxidative stress compounds. northern, primer extension, and transcription reporter fusion analyses showed that oxyr mrna is induced by 0.2 mm menad ... | 2002 | 12383513 |
| selection of patients with cystic fibrosis for lung transplantation. | lung transplantation is the most aggressive therapy available for end-stage lung disease from cystic fibrosis (cf). a new predictive survival model of cf uses demographic, fev1, nutritional, microbiologic, and acute exacerbation data to produce precise estimates of 5-year survival. the model improves the ability to select patients most likely to have survival benefit from transplantation. we discuss potential application of the survival model to four distinct groups of patients with cf: (1) cand ... | 2002 | 12394163 |
| antibiotic resistance in soil and water environments. | seven locations were screened for antibiotic-resistant bacteria using a modified agar dilution technique. isolates resistant to high levels of antibiotics were screened for r plasmids. low-level resistance (25 micro g x ml(-1)) was widespread for ampicillin, penicillin, tetracycline, vancomycin and streptomycin but not for kanamycin. resistant populations dropped sharply at high antibiotic levels, suggesting that intrinsic non-emergent mechanisms were responsible for the multiple drug resistance ... | 2002 | 12396530 |
| physiological and cellular responses of the 2,4-d degrading bacterium, burkholderia cepacia yk-2, to the phenoxyherbicides 2,4-d and 2,4,5-t. | our previous research has demonstrated that novel 43-kda dnak and 41-kda groel proteins are synthesized in burkholderia sp. yk-2 in response to sublethal concentrations of 2,4-d stress [cho et al. (2000) curr microbiol 41:33-38]. in this study, we have extended this work to examine the cellular responses of strain yk-2 to stresses induced in response to the phenoxyherbicides 2,4-d or 2,4,5-t. strain yk-2 exhibited a more sensitive response to 2,4,5-t stress than to 2,4-d stress, as shown in phys ... | 2002 | 12402082 |
| cloning and characterization of the burkholderia vietnamiensis norm gene encoding a multi-drug efflux protein. | polymyxin b-sensitive mutants in burkholderia vietnamiensis (burkholderia cepacia genomovar v) were generated with a mini-tn5 encoding tetracycline resistance. one of the transposon mutants had an insertion in the norm gene encoding a multi-drug efflux protein. expression of b. vietnamiensis norm in an escherichia coli acrab deletion mutant complemented its norfloxacin hypersensitivity, indicating that the protein functions in drug efflux. however, no effect on antibiotic sensitivity other than ... | 2002 | 12399047 |
| the use of animal infection models to study the pathogenesis of melioidosis and glanders. | the use of animal infection models is central to the study of microbial pathogenesis. in combination with genetic, immunological and antigen purification techniques, much can be learned regarding the pathogenesis of diseases caused by microorganisms. this update focuses on the recent use of animal infection models to study the pathogenesis of melioidosis and glanders. | 2002 | 12419603 |
| a novel class of self-sufficient cytochrome p450 monooxygenases in prokaryotes. | the bacillus cytochrome p450 bm3 integrates an entire p450 system in one polypeptide and represents a convenient prokaryotic model for microsomal p450s. this self-sufficient class ii p450 is also present in actinomycetes and fungi. by genome analysis we have identified additional homologues in the pathogenic species bacillus anthracis and bacillus cereus, and in ralstonia metallidurans. this analysis also revealed a novel class of putative self-sufficient p450s, p450 pfor, comprising a class i p ... | 2002 | 12419614 |
| antimicrobial resistance and dna-fingerprinting pattern of burkholderia cepacia blood isolates. | the minimum inhibitory concentrations (mics) of six antibiotics against 33 strains of burkholderia cepacia isolated from 33 patients suspected of having sepsis or bacteremia were determined. all the b. cepacia isolates were resistant to gentamicin (mic(90), >128 microg/ml), and 21.2% were resistant to imipenem (mic(90), 16 microg/ml). they were susceptible to ciprofloxacin, ceftazidime, and minocycline (mic(90), 2-8 microg/ml). the 33 blood isolates were classified into 12 types (a-l) by the pul ... | 2002 | 12525895 |
| melioidosis--a report from pondicherry, south india. | melioidosis is an acute infectious disease caused by a safety-pin-shaped gram-negative bacteria called burkholderia pseudomallei. here, we report the first case of melioidosis in a middle aged male agricultural worker, from pondicherry. the isolation of this organism from subcutaneous nodules on the extensor aspect of his limbs underlines the diversity of its clinical presentation. difficulty in identifying the organism which mimics any other non-fementing gram-negative bacilli (nfgnb) on cursor ... | 2002 | 12583480 |
| [study on the heterogeneity of lipopolysaccharides of ralstonia solanacearum]. | methods of electrophoresis in polyacrylamide gel in the system of sodium dodecyl sulphate (sds-page) with immunoblotting were used to investigate heterogeneity of lipopolysaccharides (lps) of ralstonia solanacearum (icmp 8110, 767, 5712, 7955, 7945, 7944, 8089 and 4157) which were characterized by various type structure of o-specific polysaccharides chains. when carrying out sds-page, it was determined that lps of all the investigated strains display bimodal distribution, typical of s-form lps a ... | 2002 | 12664545 |
| monoclonal antibodies against 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (kdo) and d-glycero-alpha-d-talo-oct-2-ulosonic acid (ko). | monoclonal antibodies (mabs) were obtained after immunization of mice with neoglycoconjugates containing as a ligand the disaccharide kdo(2-->4)ko or ko(2-->4)kdo, representing structural elements of the core region of lipopolysaccharides (lpss) from acinetobacter haemolyticus and burkholderia cepacia, respectively. one antibody, s67-9, bound with high specificity to ko(2-->4)kdo-bsa showing no cross reactivity with kdo(2-->4)kdo-bsa and the other antigens tested. a second mab, s68-12, bound pre ... | 2002 | 12537694 |
| burkholderia tuberum sp. nov. and burkholderia phymatum sp. nov., nodulate the roots of tropical legumes. | the taxonomic status of five root nodule isolates from tropical legumes was determined using a polyphasic taxonomic approach. two isolates were identified as b. caribensis, an organism originally isolated from soil in martinique (the french west indies). one isolate was identified as burkholderia cepacia genomovar vi, a b. cepacia complex genomovar thus far only isolated from sputum of cystic fibrosis patients. the remaining two isolates were identified as novel burkholderia species for which we ... | 2002 | 12583710 |
| study of the bacterial load in a gelatine production process focussed on bacillus and related endosporeforming genera. | gelatine is an animal protein with many industrial applications. previous studies pointed out that endosporeforming bacteria, belonging to the genus bacillus or related genera, might contaminate and survive the production process of gelatine, leading to products of low quality and safety. the aim of this study is to determine the bacterial diversity of contaminants isolated from a gelatine production chain with emphasis on aerobic endosporeforming bacteria. contaminants were isolated from sample ... | 2002 | 12583722 |
| distinguishing features of 16s rdna gene for five dominating bacterial genus observed in bioremediation. | defining a microbial community and identifying bacteria, at least at the genus level, is a first step in predicting the behavior of a microbial community in bioremediation. in biological treatment systems, the most dominating groups observed are pseudomonas, moraxella, acinetobactor, burkholderia, and alcaligenes. our interest lies in identifying the distinguishing features of these bacterial groups based on their 16s rdna sequence data, which could be used further for generating genus-specific ... | 2002 | 12614549 |
| [microbiology and cystic fibrosis]. | the respiratory infections are the rule in sick persons of cystic fibrosis. after the colonization in one's early childhood by staphylococcus aureus and haemophilus influenzae, it is established the colonization produced by the mucous strains of pseudomonas aeruginosa, the principal etiogenic of this illness, that can become to isolate in more than 95% of sick persons at three years old. that's why the etiogenic factors of this bacterium are studied, as they are toxins, pigments, enzymes, and pr ... | 2002 | 12518659 |
| single gene target bacterial identification. groel gene sequencing for discriminating clinical isolates of burkholderia pseudomallei and burkholderia thailandensis. | proper identification of burkholderia pseudomallei and burkholderia thailandensis is crucial in guiding clinical management of patients with suspected melioidosis, as more than 99% of cases of melioidosis are caused by b. pseudomallei, whereas b. thailandensis is only responsible for causing less than 1% of the cases. however, the difference between the 16s ribosomal rna gene sequences of b. pseudomallei and that of b. thailandensis is only 1%, and is therefore not discriminative enough for dist ... | 2002 | 12458120 |
| cystic fibrosis and burkholderia pseudomallei infection: an emerging problem? | we recently managed 4 patients with cystic fibrosis who had acquired burkholderia pseudomallei infection after exposure in a region of endemicity. person-to-person transmission between 2 siblings may have occurred; otherwise, the evidence suggests that cystic fibrosis may increase the likelihood of infection with this organism, and patients should be warned of this possibility and cautioned to avoid high-risk activities. | 2002 | 12471591 |
| [isolation frequency of burkholderia cepacia from cystic fibrosis patients]. | microorganisms such as pseudomonas aeruginosa, staphylococcus aureus and haemophilus influenzae frequently cause colonization and infection in airways of patients with cystic fibrosis. burkholderia cepacia has also been isolated in patients with cystic fibrosis since 1980. in this study, we aimed to determine the colonization rate of b. cepacia in 286 sputum samples obtained from 129 cystic fibrosis patients. selective media for b. cepacia were used besides the routine microbiological media in o ... | 2002 | 12476760 |
| burkholderia cepacia infection and lung transplantation. | burkholderia cepacia has emerged as an important pathogen in patients with cystic fibrosis (cf) undergoing lung transplantation. taxonomic analyses have divided b. cepacia into 7 closely related species called genomovars. the prevalence of b. cepacia infection ranges from 2% to 13% in cf patients with genomovar iii being most prevalent. risk factors for the acquisition of b. cepacia include greater severity of underlying cf, increasing age, having a sibling colonized with b. cepacia, and previou ... | 2002 | 12497545 |
| [immunobiological properties of burkholderia pseudomallei and burkholderia mallei capsular substances]. | the biopolymer composition, immunotropic and immunogenic properties of the fractions of b. pseudomallei and b. mallei were under study. the first two capsular fractions of these agents were found to be similar in their biopolymer composition that was indicative of their close relations. at the same time the causative agents of glanders proved to have decreased content of high molecular glycoproteids and lps fragments. in the causative agents of melioidosis, capsular fractions k3 and k4 were char ... | 2002 | 12506631 |
| comparison of automated and nonautomated systems for identification of burkholderia pseudomallei. | the identification of burkholderia pseudomallei, the causative agent of melioidosis, is usually not difficult in laboratories in areas where it is endemic. with the increase in international travel and the threat of bioterrorism, it has become more likely that laboratories in areas where it is not endemic could encounter this organism. the increase in the use of and dependence upon automated identification systems makes accurate identification of uncommonly encountered organisms such as b. pseud ... | 2002 | 12454163 |
| crystallization and preliminary x-ray crystallographic studies on the class ii cholesterol oxidase from burkholderia cepacia containing bound flavin. | burkholderia cepacia cholesterol oxidase (chos) is a 58.7 kda molecular-weight flavoenzyme which has been categorized as a 3beta-hydroxysteroid oxidase converting the 3beta-hydroxyl group of a range of hydroxysteroids to the corresponding ketone. analysis of enzymes with this activity has shown that two classes of cholesterol oxidase can be defined. enzymes belonging to class i contain non-covalently bound fad, whereas the class ii enzymes contain fad covalently bound to an active-site histidine ... | 2002 | 12454495 |
| crystallization and preliminary x-ray analysis of the catalase-peroxidase katg from burkholderia pseudomallei. | the bifunctional catalase-peroxidase katg encoded by the katg gene of burkholderia pseudomallei has a predicted subunit size of 81.6 kda. it shows high sequence similarity to other catalase-peroxidases of bacterial, archaebacterial and fungal origin, including 64% identity to katg from mycobacterium tuberculosis and lesser sequence similarity to members of the plant peroxidase family. crystals from this protein were grown in 16-20% peg 4000, 20% 2-methyl-2,4-pentanediol and 0.1 m sodium citrate ... | 2002 | 12454496 |
| a quantitative model for predicting enzyme enantioselectivity: application to burkholderia cepacia lipase and 3-(aryloxy)-1,2-propanediol derivatives. | we describe a new approach for predicting the enantioselectivity of enzymes towards racemic compounds. it is based on comparative binding energy (combine) analysis. the approach is used to rationalise the enantioselectivity of burkholderia cepacia lipase (bcl) towards thirteen racemic 3-(aryloxy)-1,2-propanediols in the process of acylation. according to our molecular modelling study the two 3-(aryloxy)-1,2-propanediols enantiomers bind in the bcl active site in different orientations. to derive ... | 2002 | 12463642 |
| passive protection against burkholderia pseudomallei infection in mice by monoclonal antibodies against capsular polysaccharide, lipopolysaccharide or proteins. | burkholderia pseudomallei, the aetiological agent of melioidosis, is endemic in south-east asia and northern australia, where it is an important cause of human disease. there is no vaccine available and antibiotic therapy is associated with high relapse rates. a panel of seven monoclonal antibodies (mabs) that recognise capsular polysaccharide, lipopolysaccharide or proteins was produced and their ability to protect mice passively against experimental melioidosis was evaluated. the mabs were cap ... | 2002 | 12466403 |
| purification and characterization of a novel chitinase from burkholderia cepacia strain kh2 isolated from the bed log of lentinus edodes, shiitake mushroom. | one of the chitinases secreted in the culture filtrate of a gram-negative bacteria, burkholderia cepacia strain kh2, which was isolated from the bed log of lentinus edodes, shiitake mushrooms, was purified by deae sepharose cl-6b chromatography, followed by sephacryl s-100 hr gel filtration. the purified enzyme was homogenous, determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), with an estimated molecular weight of 34,000 and an isoelectric point (pi) of 5.9. the ... | 2002 | 12469313 |
| lack of cable pili expression by cbla-containing burkholderia cepacia complex. | the burkholderia cepacia complex consists of several closely related bacterial species (or genomovars) which although generally not pathogenic for healthy individuals, contribute significantly to morbidity and mortality among persons with cystic fibrosis (cf). certain b. cepacia complex strains are more frequently recovered from cf sputum cultures than are others, and these typically reside in genomovar iii. the et12 clone is a genomovar iii strain that predominates among cf patients in canada a ... | 2002 | 12427939 |
| identification of the bacterial endosymbionts in leaf galls of psychotria (rubiaceae, angiosperms) and proposal of 'candidatus burkholderia kirkii' sp. nov. | this paper reports the identification of bacterial endosymbionts inhabiting the leaf galls of psychotria kirkii. a phylogenetic approach was used to reveal the identity of these as yet uncultivable bacterial endophytes. based on the analysis of 16s rdna sequences, evolutionary trees were constructed that place the endosymbiont in the genus burkholderia. low levels of sequence identity and rather large evolutionary distances to the closest validly named relatives indicate that these symbiotic bac ... | 2002 | 12508863 |
| pharmacokinetics and efficacies of liposomal and conventional formulations of tobramycin after intratracheal administration in rats with pulmonary burkholderia cepacia infection. | the objective of the present study was to determine the pharmacokinetics and efficacies of liposomal and conventional formulations of tobramycin against burkholderia cepacia in a model of chronic lung infection. male sprague-dawley rats were inoculated intratracheally with 10(6) cfu of a very resistant strain of b. cepacia (strain bc 1368; mic, 128 micro g/ml) to establish lung infection. a 1,200- micro g dose of tobramycin was administered intratracheally as a liposomal formulation and as a con ... | 2002 | 12435676 |
| interventions for treating melioidosis. | melioidosis is an infectious disease that occurs in tropical regions, particularly in thailand. it is caused by the bacterium burkholderia pseudomallei and is a serious condition which can be fatal. beta-lactam antibiotics have dramatically reduced the risk of death, but mortality still remains high. | 2002 | 12519552 |
| simplified para-nitrophenyl palmitate assay for lipases and esterases. | 2002 | 12441161 | |
| respiratory infections. | 2002 | 12448673 | |
| immobilized lipase-catalysed production of alkyl esters of restaurant grease as biodiesel. | simple alkyl ester derivatives of restaurant grease were prepared using immobilized lipases as biocatalysts. the lipases studied included those of thermomyces lanuginosa and candida antarctica supported on granulated silica (gran- t.l. and gran- c.a., respectively), c. antarctica supported on a macroporous acrylic resin (sp435) and pseudomonas cepacia immobilized within a phyllosilicate sol-gel matrix (im ps-30). all alcoholysis reactions were carried out in solvent-free media employing a one-st ... | 2002 | 12452801 |
| diversity of transconjugants that acquired plasmid pjp4 or pemt1 after inoculation of a donor strain in the a- and b-horizon of an agricultural soil and description of burkholderia hospita sp. nov. and burkholderia terricola sp. nov. | we examined the diversity of transconjugants that acquired the catabolic plasmids pjp4 or pemt1, which encode degradation of 2,4-dichlorophenoxyacetic acid (2,4-d), in microcosms with agricultural soil inoculated with a donor strain (dejonghe, w., goris, j., el fantroussi, s., höfte, m., de vos, p., verstraete, w., and top, e. m. appl. environ. microbiol. 2000, p. 3297-3304). using repetitive element pcr fingerprinting, eight different rep-clusters and six separate isolates could be discriminate ... | 2002 | 12421072 |
| a possible pitfall in the identification of burkholderia mallei using molecular identification systems based on the sequence of the flagellin flic gene. | amotile burkholderia mallei and motile burkholderia pseudomallei display a high similarity with regard to phenotype and clinical syndromes, glanders and melioidosis. the aim of this study was to establish a fast and reliable molecular method for identification and differentiation. despite amotility, the gene of the filament forming flagellin (flic) could be completely sequenced in two b. mallei strains. only one mutation was identified discriminating between b. mallei and b. pseudomallei. a poly ... | 2002 | 12423776 |
| evaluation of trna intergenic length polymorphism (tdna-pcr) for the differentiation of the members of the burkholderia cepacia complex. | nowadays, tentative identification of b. cepacia complex bacteria in routine diagnostic laboratories is based on a combination of selective media, conventional biochemical reactions, commercial test systems and pcr-based assays. some of these assays have the capacity to discriminate reliably among several members of the b. cepacia complex, however one single method differentiating all b. cepacia-like organisms is not available. in this study, the applicability of tdna-pcr for the differentiation ... | 2002 | 12421075 |
| the partially degraded lipopolysaccharide of burkholderia cepacia and ornithine-containing lipids derived from some gram-negative bacteria are useful complex lipid adjuvants. | the partially degraded lipopolysaccharide of burkholderia cepacia (lpsdegr) and the ornithine-containing lipids were purified from some bacteria. the substances were developed as complex lipid adjuvants, because they have weak toxicity and are able to activate the immune systems of the living body. after various toxoid antigens such as pertussis toxoid, diphtheria toxoid and tetanus toxoid were mixed with the complex lipid adjuvants, the mixtures were administered to mice subcutaneously. antitox ... | 2002 | 12423768 |
| evaluation of species-specific reca-based pcr tests for genomovar level identification within the burkholderia cepacia complex. | the burkholderia cepacia complex presently comprises nine genomovars: b. cepacia (genomovar i), b. multivorans (genomovar ii), b. cepacia genomovar iii, b. stabilis (genomovar iv), b. vietnamiensis (genomovar v), b. cepacia genomovar vi, b. ambifaria (genomovar vii), b. anthina (genomovar viii) and b. pyrrocinia (genomovar ix). strains of each genomovar can colonise the respiratory tract of cystic fibrosis (cf) patients. however, the majority of infections in cf patients are caused by b. multivo ... | 2002 | 12448677 |
| arbuscular mycorrhizal fungi: a specialised niche for rhizospheric and endocellular bacteria. | arbuscular mycorrhizal (am) fungi produce an extensive hyphal network which develops in the soil, producing a specialised niche for bacteria. the aim of this paper is to review briefly the interactions shown by these symbiotic fungi with two bacterial groups: (i) the plant-growth promoting rhizobacteria (pgprs) which are usually associated with fungal surfaces in the rhizosphere, and (ii) a group of endocellular bacteria, previously identified as being related to burkholderia on the basis of the ... | 2002 | 12448735 |
| statistical medium optimization and production of a hyperthermostable lipase from burkholderia cepacia in a bioreactor. | statistical medium optimization for maximum production of a hyperthermostable lipase from burkholderia cepacia and its validation in a bioreactor. | 2002 | 12452948 |
| directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. | trichloroethylene (tce) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. directed evolution was used to increase the activity of toluene ortho-monooxygenase (tom) of burkholderia cepacia g4 for both chlorinated ethenes and naphthalene oxidation. when expressed in escherichia coli, the variant tom-green degraded tce (2.5 +/- 0.3 versus 1.39 +/- 0.05 nmol/min/mg of protein), 1,1-dichloroethylene, and trans-dichloroethylene ... | 2002 | 11751810 |
| colony hybridization of bacterial isolates with burkholderia cepacia-specific probes. | 2002 | 11692862 | |
| in situ hybridization of burkholderia cepacia cells. | 2002 | 11692863 | |
| sorption versus biomineralization of pb(ii) within burkholderia cepacia biofilms. | x-ray spectroscopy measurements have been combined with macroscopic uptake data and transmission electron microscopy (tem) results to show that pb(ii) uptake by burkholderia cepacia is due to simultaneous sorption and biomineralization processes. x-ray microprobe mapping of b. cepacia biofilms formed on alpha-al2o3 surfaces shows that pb(ii) is distributed heterogeneously throughout the biofilms because of the formation of pb "hot spots". exafs data and tem observations show that the enhanced pb ... | 2003 | 12564901 |
| construction of a deep-rough mutant of burkholderia cepacia atcc 25416 and characterization of its chemical and biological properties. | burkholderia cepacia is a bacterium with increasing importance as a pathogen in patients with cystic fibrosis. the deep-rough mutant ko2b was generated from b. cepacia type strain atcc 25416 by insertion of a kanamycin resistance cassette into the gene waac encoding heptosyltransferase i. mass spectrometric analysis of the de-o-acylated lipopolysaccharide (lps) of the mutant showed that it consisted of a bisphosphorylated glucosamine backbone with two 3-hydroxyhexadecanoic acids in amide-linkage ... | 2003 | 12427755 |
| a comparison of pulmonary exacerbations with single and multiple organisms in patients with cystic fibrosis and chronic burkholderia cepacia infection. | to compare pulmonary exacerbations with single and multiple organisms in patients with chronic burkholderia cepacia infection. | 2003 | 12504610 |
| biodefense-driven murine model of pneumonic melioidosis. | a whole-body mouse model of pneumonic melioidosis was established for future evaluation of biodefense vaccine candidates. the aerosol 50% lethal doses of burkholderia pseudomallei strain 1026b for balb/c and c57bl/6 mice and the times to death, dissemination in organs, and tissue loads after exposure of the mice to low- and high-dose aerosols are reported. in addition, rpsl mutant backgrounds were attenuated in this acute model of disease. | 2003 | 12496217 |
| characterization of amplified polymerase chain reaction glnb and nifh gene fragments of nitrogen-fixing burkholderia species. | to clone and sequence polymerase chain reaction (pcr)-amplified glnb and nifh genes of the nitrogen-fixing bacteria burkholderia brasilensis strain m130, b. tropicalis strain ppe8 and b. kururiensis strain kp23. | 2003 | 12535125 |
| phagocyte nadph oxidase, but not inducible nitric oxide synthase, is essential for early control of burkholderia cepacia and chromobacterium violaceum infection in mice. | reactive oxygen and nitrogen intermediates have critical, partially overlapping roles in host defense against a variety of pathogens. using mice deficient in generating phagocyte superoxide (p47(phox)(-/-)) and mice deficient in generating inducible nitric oxide synthase (inos(-/-)), we examined the roles of these reactive species in host defense against burkholderia cepacia and chromobacterium violaceum, organisms known to have unusual virulence in chronic granulomatous disease. intraperitoneal ... | 2003 | 12496167 |
| attenuated virulence of a burkholderia cepacia type iii secretion mutant in a murine model of infection. | type iii secretion systems are utilized by a number of gram-negative bacterial pathogens to deliver virulence-associated proteins into host cells. using a pcr-based approach, we identified homologs of type iii secretion genes in the gram-negative bacterium burkholderia cepacia, an important pulmonary pathogen in immunocompromised patients and patients with cystic fibrosis. one of the genes, designated bscn, encodes a member of a family of atp-binding proteins believed to generate energy driving ... | 2003 | 12595458 |
| identification of dna markers of tobacco linked to bacterial wilt resistance. | bacterial wilt caused by ralstonia solanacearum is one of the most destructive soil-borne diseases in the world. breeding resistant commercial varieties of tobacco is difficult because most donor candidates' resistance is controlled by polygenes. in this paper, we demonstrate the identification of useful dna markers for bacterial wilt-resistant tobacco breeding. one hundred and seventeen markers were identified by the amplified fragment length polymorphism (aflp) method between w6, a burley vari ... | 2003 | 12596008 |
| reinfection, rather than persistent infection, in patients with chronic granulomatous disease. | chronic granulomatous disease (cgd) is characterized by severe recurrent infections with staphylococcus aureus, certain gram-negative rods, nocardia species, and fungi. when infections with the same species recur, they may represent relapses or new infections. we collected organisms from infections that occurred between 1992 and 2000 in patients with cgd and determined the biochemical phenotypes, in vitro antibiotic susceptibility patterns, and pulsed-field gel electrophoresis (pfge) patterns of ... | 2003 | 12599059 |
| molecular epidemiology of burkholderia species. | although most species in the genus burkholderia are not pathogenic for healthy persons, a few are capable of causing severe, life threatening infection. b. mallei and b. pseudomallei are the causative agents of glanders and melioidosis, respectively. interest in these species has increased recently owing to their potential for use as agents of bioterrorism. b. cepacia emerged during the past two decades as an important opportunistic pathogen among persons with certain underlying diseases. person ... | 2003 | 12456343 |
| ubiquity of putative type iii secretion genes among clinical and environmental burkholderia pseudomallei isolates in northern australia. | type iii secretion (ttsi) genes of an hrp (hypersensitivity response and pathogenicity)-like locus were present in all 116 northern australian burkholderia pseudomallei isolates tested but were not detected in other common environmental burkholderia species. pcr of tts1 genes may prove valuable as a diagnostic test [corrected]. | 2003 | 12574307 |
| biosynthesis and local sequence specific degradation of poly(3-hydroxyvalerate-co-4-hydroxybutyrate) in hydrogenophaga pseudoflava. | a novel copolymer that consisted of 3-hydroxyvalerate and 4-hydroxybutyrate, p(3hv-co-4hb), was synthesized in hydrogenophaga pseudoflava by growing it in media containing gamma-valerolactone and gamma-butyrolactone as a carbon source. the monomer ratio in the copolymer was changed by altering the feed ratio of the two lactones. the cultivation technique was composed of three steps: the first-step for high cell production in luria-bertani medium, the second-step for intracellular degrading remov ... | 2003 | 12523844 |
| identification of genes encoding secreted proteins using mini-ophoa mutagenesis. | 2003 | 12491898 | |
| in vitro synergy studies using aztreonam and fluoroquinolone combinations against six species of gram-negative bacilli. | combination antimicrobial therapy is often necessary to eradicate infections caused by gram-negative bacteria. | 2003 | 12714810 |
| behavior of silica aerogel networks as highly porous solid solvent media for lipases in a model transesterification reaction. | highly porous silica aerogels with differing balances of hydrophobic and hydrophilic functionalities were studied as a new immobilization medium for enzymes. two types of lipases from candida rugosa and burkholderia cepacia were homogeneously dispersed in wet gel precursors before gelation. the materials obtained were compared in a simple model reaction: transesterification of vinyl laurate by 1-octanol. to allow a better comparison of the hydrophobic/hydrophilic action of the solid, very open a ... | 2003 | 12616634 |
| [melioidosis]. | 2003 | 12722256 | |
| comparative in vitro potency of amoxycillin-clavulanic acid and four oral agents against recent north american clinical isolates from a global surveillance study. | the in vitro activity of amoxycillin-clavulanic acid was compared with four comparator oral antimicrobial agents; ampicillin, azithromycin, cefuroxime and trimethoprim-sulphamethoxazole against 4536 recent clinical isolates covering 29 species isolated in the us and canada between 1997 and 1999. based upon minimum inhibitory concentrations (mics), amoxycillin-clavulanic acid was the most active agent against many gram-positive species and phenotypes including methicillin susceptible staphylococc ... | 2003 | 12727075 |
| differential scanning calorimetry of the effects of ca2+ on the thermal unfolding of pseudomonas cepacia lipase. | thermal unfolding of p. cepacia lipase was observed by adiabatic differential scanning microcalorimetry in the absence and presence of calcium ions at ph 8, and thermodynamic parameters of unfolding were evaluated to analyze the unfolding mechanism of the enzyme. the temperature of unfolding was higher at higher concentrations of ca2+. from the ca2+ concentration-dependence of the unfolding temperature, the number of calcium ions that dissociated from the enzyme molecule upon unfolding was estim ... | 2003 | 12619699 |
| regulation of the katg-dpsa operon and the importance of katg in survival of burkholderia pseudomallei exposed to oxidative stress. | homologues of the catalase-peroxidase gene katg and the gene for the non-specific dna binding protein dpsa were identified downstream of oxyr in burkholderia pseudomallei. northern experiments revealed that both katg and dpsa are co-transcribed during oxidative stress. under conditions where the katg promoter is not highly induced, dpsa is transcribed from a second promoter located within the katg-dpsa intergenic region. a katg insertion mutant was found to be hypersensitive to various oxidants. ... | 2003 | 12729890 |
| energy-generating enzymes of burkholderia cepacia and their interactions with macrophages. | we previously demonstrated that several clinical and environmental isolates of burkholderia cepacia secreted atp-utilizing enzymes to the medium; the secretion of these enzymes by cystic fibrosis lung isolate strain 38 was shown to be greatly enhanced in the presence of alpha(2)-macroglobulin. fractionation of the growth medium of cystic fibrosis isolate strain 71 belonging to genomovar i demonstrated the presence of two additional proteins, homologues of pseudomonas aeruginosa azurin and cytoch ... | 2003 | 12730177 |
| the wbia locus is required for the 2-o-acetylation of lipopolysaccharides expressed by burkholderia pseudomallei and burkholderia thailandensis. | burkholderia pseudomallei and burkholderia thailandensis express similar o-antigens (o-ps ii) in which their 6-deoxy-alpha-l-talopyranosyl (l-6dtalp) residues are variably substituted with o-acetyl groups at the o-2 or o-4 positions. in previous studies we demonstrated that the protective monoclonal antibody, pp-ps-w, reacted with o-ps ii expressed by wild-type b. pseudomallei strains but not by a b. pseudomallei wbia null mutant. in the present study we demonstrate that wbia activity is require ... | 2003 | 12586411 |
| the humoral immune response in melioidosis patients during therapy. | this study was undertaken to identify and quantify the class and subclass antibody responses to the culture filtrate antigen (cfa) of burkholderia pseudomallei in melioidosis patients under long-term maintenance or eradication therapy. | 2003 | 12590329 |
| specific detection of pseudomonas spp. in milk by fluorescence in situ hybridization using ribosomal rna directed probes. | pseudomonas spp. are considered the most important milk spoilage organisms. here we describe development of a fluorescence in situ hybridization (fish) probe specific for detection and enumeration of pseudomonas spp. in milk. | 2003 | 12694460 |
| a case of imported melioidosis presenting as prostatitis. | we report a case of melioidosis in a previously healthy belgian man. he presented with septicemia and prostatic abscesses 1 week after a trip to vietnam. burkholderia pseudomallei was isolated from multiple hemocultures. he was treated successfully with intravenous ceftazidime and trimethoprim-sulfamethoxazole, followed by a per-oral maintenance therapy of amoxicillin-clavulanate with supplementary amoxicillin. there was no need for surgical drainage. this is the second reported case of melioido ... | 2003 | 12590337 |
| in vitro activity of gatifloxacin alone and in combination with cefepime, meropenem, piperacillin and gentamicin against multidrug-resistant organisms. | to study the in vitro interaction of gatifloxacin in combination with gentamicin and with the beta-lactams cefepime, meropenem and piperacillin against clinical isolates of stenotrophomonas maltophilia, pseudomonas aeruginosa, burkholderia cepacia, extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae, vancomycin-resistant enterococcus faecium (vre) and methicillin-resistant staphylococcus aureus (mrsa). | 2003 | 12697632 |
| a family history of deoxyribonuclease ii: surprises from trichinella spiralis and burkholderia pseudomallei. | deoxyribonuclease iialpha (dnase iialpha) is an acidic endonuclease found in lysosomes and nuclei, and it is also secreted. though its caenorhabditis elegans homolog, nuc-1, is required for digesting dna of apoptotic cell corpses and dietary dna, it is not required for viability. however, dnase iialpha is required in mice for correct development and viability, because undigested cell corpses lead to lesions throughout the body. recently, we showed that, in contrast to previous reports, active dn ... | 2003 | 12594037 |
| analysis of the quorum-sensing regulon of the opportunistic pathogen burkholderia cepacia h111 by proteomics. | burkholderia cepacia h111, an important pathogen for persons suffering from cystic fibrosis, employs a quorum-sensing (qs) system, cep, to control expression of virulence factors as well as the formation of biofilms. the qs system is thought to ensure that pathogenic traits are only expressed when the bacterial population density is high enough to overwhelm the host before it is able to mount an efficient response. in this study, we compared the protein pattern of the intracellular, extracellula ... | 2003 | 12601746 |
| suppression-subtractive hybridisation reveals variations in gene distribution amongst the burkholderia cepacia complex, including the presence in some strains of a genomic island containing putative polysaccharide production genes. | some strains of the burkholderia cepacia complex, including the et12 lineage, have been implicated in epidemic spread amongst cystic fibrosis (cf) patients. suppression-subtractive hybridisation was used to identify genomic regions within strain j2315 (et12 lineage; genomovar iiia) that were absent from a non-transmissible genomovar iiib strain. sequence data from 15 subtracted clones were used to interrogate the genome sequence of strain j2315 and identify genomic regions incorporating the subt ... | 2003 | 12610727 |
| burkholderia is highly resistant to human beta-defensin 3. | the bactericidal activity of the novel beta-defensin hbd-3 against 28 species and 55 strains of gram-positive cocci and gram-negative fermentative and nonfermentative rods was tested. all strains proved to be highly or intermediately susceptible to hbd-3 (minimal bactericidal concentration [mbc], </=50 micro g/ml), except for burkholderia cepacia, for all 23 tested strains of which mbcs were >100 micro g/ml. | 2003 | 12709350 |
| colonial morphology of burkholderia cepacia complex genomovar iii: implications in exopolysaccharide production, pilus expression, and persistence in the mouse. | the purpose of this study was to determine the role of colonial morphology of burkholderia cepacia complex (bcc) organisms in pathogenicity in a mouse model of pulmonary infection. bcc strain c1394 was rapidly cleared by leukopenic mice after intranasal challenge, whereas a spontaneous variant (c1394mp2) that was indistinguishable from the parent strain by genetic typing persisted in the lungs and differed in colonial morphology. the parent strain had a matte colonial phenotype, made scant exopo ... | 2003 | 12540572 |
| flagellum-mediated adhesion by burkholderia pseudomallei precedes invasion of acanthamoeba astronyxis. | in this study we investigated the role of the bacterial flagellum in burkholderia pseudomallei entry to acanthamoeba astronyxis trophozoites. b. pseudomallei cells were tethered to the external amoebic surface via their flagella. mm35, the flagellum-lacking flic knockout derivative of b. pseudomallei nctc 1026b did not demonstrate flagellum-mediated endocytosis in timed coculture, confirming that an intact flagellar apparatus assists b. pseudomallei entry into a. astronyxis. | 2003 | 12654857 |
| characterization of extradiol dioxygenases from a polychlorinated biphenyl-degrading strain that possess higher specificities for chlorinated metabolites. | recent studies demonstrated that 2,3-dihydroxybiphenyl 1,2-dioxygenase from burkholderia sp. strain lb400 (dhbdlb400; ec 1.13.11.39) cleaves chlorinated 2,3-dihydroxybiphenyls (dhbs) less specifically than unchlorinated dhb and is competitively inhibited by 2',6'-dichloro-2,3-dihydroxybiphenyl (2',6'-dicl dhb). to determine whether these are general characteristics of dhbds, we characterized dhbdp6-i and dhbdp6-iii, two evolutionarily divergent isozymes from rhodococcus globerulus strain p6, ano ... | 2003 | 12562795 |
| cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from burkholderia cepacia in escherichia coli. | we have cloned a 1620-nucleotide gene encoding the catalytic subunit (alpha subunit) of a thermostable glucose dehydrogenase (gdh) from burkholderia cepacia. the fad binding motif was found in the n-terminal region of the alpha subunit. the deduced primary structure of the alpha subunit showed about 48% identity to the catalytic subunits of sorbitol dehydrogenase (sdh) from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases (2kgdh) from erwinia herbicola and pantoea citrea. the alpha su ... | 2003 | 12573242 |
| population structure analysis of burkholderia cepacia genomovar iii: varying degrees of genetic recombination characterize major clonal complexes. | infection with bacterial species belonging to the burkholderia cepacia complex contribute significantly to morbidity and mortality in persons with cystic fibrosis (cf). the majority of isolates recovered from cf patients belong to b. cepacia genomovar iii and several distinct 'epidemic' strains have been described. this study examined the population structure of b. cepacia genomovar iii by using multilocus restriction typing, indexing allelic variation at five chromosomal genes by restriction an ... | 2003 | 12576582 |
| use of the e test to assess synergy of antibiotic combinations against isolates of burkholderia cepacia-complex from patients with cystic fibrosis. | treatment of burkholderia cepacia-complex infections in cystic fibrosis patients is problematic, since the microorganism is often resistant to most antimicrobial agents. in this study, the epsilometer test, or e test, was used to assess the activity of antimicrobial combinations against burkholderia cepacia-complex. in a preliminary evaluation, the e test was compared to the checkerboard method using 10 test organisms. synergy testing by the e test was then performed on 131 clinical isolates of ... | 2003 | 12582741 |
| molecular cloning and complementation analysis of nifv gene from frankia euik1 strain. | the nifv gene from the frankia euik1 strain, a symbiont of elaeagnus umbellata, was cloned and a complementation test using the klebsiella pneumoniae nifv mutant was performed to verify its function. the nifv orf consists of 1245 bp, which encodes 414 amino acids. however, the putative promoter and shine-dalgarno sequences were not found in the 5' region of the orf. the frankia euik1 nifv orf showed about a 70% nucleotide identity and 80% amino acid similarity with that of frankia sp. fac1. in t ... | 2003 | 12661757 |
| horizontal transfer of phnac dioxygenase genes within one of two phenotypically and genotypically distinctive naphthalene-degrading guilds from adjacent soil environments. | several distinct naphthalene dioxygenases have been characterized to date, which provides the opportunity to investigate the ecological significance, relative distribution, and transmission modes of the different analogs. in this study, we showed that a group of naphthalene-degrading isolates from a polycyclic aromatic hydrocarbon (pah)-contaminated hillside soil were phenotypically and genotypically distinct from naphthalene-degrading organisms isolated from adjacent, more highly contaminated s ... | 2003 | 12676698 |
| automated identification systems and burkholderia pseudomallei. | 2003 | 12682195 | |
| a transposase-like gene isolated from burkholderia pseudomallei by ddrt-pcr technique. | 2003 | 12682867 | |
| contamination of trypan blue with burkholderia cepacia in a cornea bank. | to describe burkholderia cepacia contamination of a cornea bank and the measures taken to identify and eliminate the source of infection. | 2003 | 12683512 |
| burkholderia cenocepacia sp. nov.--a new twist to an old story. | dna-dna hybridisation experiments between isolates representing burkholderia cepacia genomovar iii reca lineages iiia and iiib reinforced the classification of both phylogenetic subgroups as a single genospecies, distinct from b. cepacia (genomovar i). a formal classification of b. cepacia genomovar iii encompassing the reca lineages iiia and iiib, and the new reca lineages iiic and iiid, as b. cenocepacia sp. nov., with lmg 16656 as the type strain, is proposed. | 2003 | 12648723 |
| adaptation to nickel spiking of bacterial communities in neocaledonian soils. | adaptation to nickel of bacterial communities of two extreme neocaledonian soils (an ultramafic soil and an acidic soil) was investigated by nickel spiking and compared with adaptation in a non-neocaledonian soil used as reference. soil microcosms were amended with nickel chloride (nicl2), and bacterial community structure was analysed with the ribosomal intergenic spacer analysis (risa) technique. then, bacterial populations that respond to nickel stress were identified by cloning and sequencin ... | 2003 | 12542708 |
| lack of correlation between o-serotype, bacteriophage susceptibility and genomovar status in the burkholderia cepacia complex. | the burkholderia cepacia complex comprises at least nine phylogenetically related genomic species (genomovars) which cause life-threatening infection in immunocompromised humans, particularly individuals with cystic fibrosis or chronic granulomatous disease. prior to recognition that 'b. cepacia' comprise multiple species, in vitro studies revealed that the lipopolysaccharide (lps) of these gram-negative bacteria is strongly endotoxic. in this study, we used 117 b. cepacia complex isolates to de ... | 2003 | 12628542 |
| a comparison of antibiotic regimens in the treatment of acute melioidosis in a mouse model. | melioidosis is caused by the gram-negative bacillus burkholderia pseudomallei. most clinical reports of disease are from south-east asia and northern australia. the organism is intrinsically resistant to most commonly available antibiotics. standard therapy includes ceftazidime either alone or in combination with co-trimoxazole. the clinical advantage in adding co-trimoxazole has never been determined; nor has the activity of newer, fourth-generation cephalosporins, such as cefepime, been studie ... | 2003 | 12493790 |