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morphological and molecular characterization of myxobolus sheyangensis n. sp. (myxosporea: myxobolidae) with intralamellar sporulation in allogynogenetic gibel carp, carassius auratus gibelio (bloch) in china.allogynogenetic gibel carp is one of the most important freshwater cultured species in china. however, myxosporidiosis represents a severe threat to prevent the sustainable development of aquaculture of this species. during the investigation of myxosporean diversity of reared allogynogenetic gibel carp in east china, a new myxosporean with typical characteristics of myxobolus was found from 169 out of 210 (80.4 %) examined samples, designated as myxobolus sheyangensis n. sp. by combing comparati ...201627225002
intra-oral acid production associated with eating whole or pulped raw fruits.the hypotheses that raw fruits, whether whole or pulped, were cleared rapidly from the mouth and that the sugars in the whole and pulped fruits are fermented with equal efficiency to acids by the oral microflora were tested in this study. groups of 7-9 adult subjects chewed 10 g of raw, whole or pulped fruit (apple, banana, orange, pear and pineapple) for 1 min and whole, unstimulated saliva samples were collected during the following 60-min interval. each saliva sample was assayed for the conce ...201615181333
pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method.many fruits soften during ripening, which is important commercially and in rendering the fruit attractive to seed-dispersing animals. cell-wall polysaccharide hydrolases may contribute to softening, but sometimes appear to be absent. an alternative hypothesis is that hydroxyl radicals ((•)oh) non-enzymically cleave wall polysaccharides. we evaluated this hypothesis by using a new fluorescent labelling procedure to 'fingerprint' (•)oh-attacked polysaccharides.201626865506
comparative genomic analysis of the grf genes in chinese pear (pyrus bretschneideri rehd), poplar (populous), grape (vitis vinifera), arabidopsis and rice (oryza sativa).growth-regulating factors (grfs) are plant-specific transcription factors that have important functions in regulating plant growth and development. previous studies on grf family members focused either on a single or a small set of genes. here, a comparative genomic analysis of the grf gene family was performed in poplar (a model tree species), arabidopsis (a model plant for annual herbaceous dicots), grape (one model plant for perennial dicots), rice (a model plant for monocots) and chinese pea ...201627933074
promotion of flowering by apple latent spherical virus vector and virus elimination at high temperature allow accelerated breeding of apple and pear.plant viral vectors are superior tools for genetic manipulation, allowing rapid induction or suppression of expression of a target gene in plants. this is a particularly effective technology for use in breeding fruit trees, which are difficult to manipulate using recombinant dna technologies. we reported previously that if apple seed embryos (cotyledons) are infected with an apple latent spherical virus (alsv) vector (alsv-atft/mdtfl1) concurrently expressing the arabidopsis thaliana florigen (a ...201626941750
traditional chinese medicine, food therapy, and hypertension control: a narrative review of chinese literature.despite the lack of english literature about traditional chinese medicine (tcm) food therapy, there is abundant chinese literature about the application of food therapy for hypertension control. this paper summarizes basic concepts of tcm, the principles of food therapy and its application for hypertension control according to chinese literature. in tcm, food is conceptualized according to both nutritional and functional aspects, and can be used to treat illnesses. four principles of tcm food th ...201627852126
predicting the dynamic impact behaviour of spray droplets on flat plant surfaces.the dynamic impact behaviour of water droplets on plant surfaces was investigated based on a multiphase computational fluid dynamics (cfd) model. the study was conducted using the volume of fluid (vof) approach. the static contact angle of water droplets on leaf surfaces of different plants (apple, pear, leek and cabbage) was measured and found to vary between 54.9 and 138.2°. impact experiments were conducted by monitoring the flow and impact characteristics of water droplets on leaves in still ...201627501228
iron-modulated pseudocyst formation in tritrichomonas foetus.iron is an essential element for the survival of trichomonads during host-parasite interaction. the availability of this metal modulates several metabolic pathways of the parasites and regulates the expression of virulence factors such as adhesins and proteolytic enzymes. in this study, we investigated the effect of iron depletion on the morphology and life cycle of tritrichomonas foetus. scanning and transmission electron microscopy analyses revealed that depletion of iron from the culture medi ...201627253439
xylella taiwanensis sp. nov., causing pear leaf scorch disease.a gram-stain-negative, nutritionally fastidious bacterium (pls229t) causing pear leaf scorch was identified in taiwan and previously grouped into xylella fastidiosa. yet, significant variations between pls229t and xylellafastidiosa were noted. in this study, pls229t was evaluated phenotypically and genotypically against representative strains of xylellafastidiosa, including strains of the currently known subspecies of xylellafastidiosa, xylella fastidiosa subsp. multiplex and 'xylella fastidiosa ...201627530392
novel approach of using nutraceutic-directed caloric antioxidant density and ion-ratio for evaluating fruit's health quality.seven kinds of indigenous fruits and five imported fruits were compared for their "health quality." methods including the calorific, antioxidant, and ion ratios were carried out. results indicated the order of content (in mg/100g) was: ca(2+) , murcott orange (218.2) > kiwifruit (200.0) > pineapple (138.5) > golden kiwi (117.6); mg(2+) , pitaya (192.2), banana (88.0), kiwifruit (63.4), and golden kiwi (58.4); zn(2+) , pitaya (19.53) > pear (10.8) > kiwifruit (6.09) > irwin mango (4.58). cu(2+) , ...201627467811
population genetic analyses of the fungal pathogen colletotrichum fructicola on tea-oil trees in china.the filamentous fungus colletotrichum fructicola is found in all five continents and is capable of causing severe diseases in a number of economically important plants such as avocado, fig, cocoa, pear, and tea-oil trees. however, almost nothing is known about its patterns of genetic variation and epidemiology on any of its host plant species. here we analyzed 167 isolates of c. fructicola obtained from the leaves of tea-oil tree camellia oleifera at 15 plantations in seven chinese provinces. mu ...201627299731
migrating zooids allow the dispersal of fredericella sultana (bryozoa) to escape from unfavourable conditions and further spreading of tetracapsuloides bryosalmonae.fredericella sultana (bryozoa: phylactolaemata) is a primary host in the two-host life cycle of the myxozoan parasite tetracapsuloides bryosalmonae, the etiological agent of proliferative kidney disease (pkd) in salmonids. overtly infected f. sultana colonies were collected from river kamp (lower austria), following the first pkd outbreak affecting autochthonous brown trout (salmo trutta) in austria. zooids cultured under unfavourable conditions, e.g. hypertrophication or sudden temperature chan ...201627546864
genome-wide identification and comparative analysis of the cation proton antiporters family in pear and four other rosaceae species.the monovalent cation proton antiporters (cpas) play essential roles in plant nutrition, development, and signal transduction by regulating ion and ph homeostasis of the cell. the cpas of plants include the na(+)/h(+) exchanger, k(+) efflux antiporter, and cation/h(+) exchanger families. however, currently, little is known about the cpa genes in rosaceae species. in this study, 220 cpa genes were identified from five rosaceae species (pyrus bretschneideri, malus domestica, prunus persica, fragar ...201627193473
studies of the macroscopic and microscopic morphology (hippocampus) of brain in vencobb broiler.the aim of this study was to study the anatomy of different parts of brain and histology of hippocampus of vencobb broiler chicken.201627284228
distribution of α-transducin and α-gustducin immunoreactive cells in the chicken (gallus domesticus) gastrointestinal tract.the expression and distribution patterns of the taste signaling molecules, α-gustducin (gαgust) and α-transducin (gαtran) g-protein subunits, were studied in the gastrointestinal tract of the chicken (gallus domesticus) using the immunohistochemical method. gαgust and gαtran immunoreactive (-ir) cells were observed in the mucosal layer of all examined segments, except the esophagus, crop, and the saccus cranialis of the gizzard. the highest numbers of gαgust and gαtran-ir cells were found in the ...201626957624
three new and a known species of the genus <i>proleptonchus</i> lordello, 1955 (nematoda: leptonchidae) with a diagnostic compendium of the genus.this paper deals with the description of three new and one known species of the genus proleptonchus lordello, 1955. proleptonchus kazirangus n. sp. from kaziranga national park, india is characterized by having 1.08-1.45 mm long body; cap-like, offset, lip region; 7-8 µm long odontostyle and 10-12 µm long odontophore; short, pear-shaped pharyngeal bulb offset by a constriction, occupying about 18-21% of total neck length; female genital system mono-prodelphic; pars dilatata uteri with distinctly ...201627988759
genomics of pear and other rosaceae fruit trees.the family rosaceae includes many economically important fruit trees, such as pear, apple, peach, cherry, quince, apricot, plum, raspberry, and loquat. over the past few years, whole-genome sequences have been released for chinese pear, european pear, apple, peach, japanese apricot, and strawberry. these sequences help us to conduct functional and comparative genomics studies and to develop new cultivars with desirable traits by marker-assisted selection in breeding programs. these genomics reso ...201627069399
thorectinae (porifera: demospongiae: dictyoceratida) from northeastern brazil: two new species and transfer of scalarispongia cincta (boury-esnault, 1973) to the genus thorecta lendenfeld, 1888.the genus scalarispongia was raised to gather species formerly allocated in cacospongia with rectangular fibrous skeleton of cored primary fibers and uncored secondary fibers. species of the genus thorecta have a similar skeletal architecture, but the presence of a dermal armour distinguishes them from scalarispongia. here we describe two new species of scalarispongia from northeastern brazil and transfer scalarispongia cincta to thorecta cincta. scalarispongia tubulata sp. nov. is thick encrust ...201627811660
further studies on south eastern asian luciolinae: 1. sclerotia ballantyne, <br />a new genus of fireflies with back swimming larvae 2. triangulara pimpasalee, a new genus from thailand (coleoptera: lampyridae).sclerotia ballantyne gen. nov. is established for seven species. sclerotia aquatilis (thancharoen, 2007), scl. brahmina (bourgeois, 1890), scl. carinata (gorham, 1880), scl. flavida (hope, 1845), scl. seriata (olivier, 1891), and scl. substriata (gorham, 1880) are transferred from luciola laporte. sclerotia fui sp. nov. from china is described as new. luciola cingulata olivier, 1885 is synonymised with luciola substriata gorham. luciola formosana pic, 1916 is removed from synonymy with luciola s ...201627701260
two new species of chromadoridae (chromadorida: nematoda) from the east china sea.two new species of chromadoridae, a family of free-living marine nematodes, are described from intertidal sediments of the east china sea. ptycholaimellus pirus sp. nov. is characterized by having a cuticle with six longitudinal rows of double dots and long somatic setae, relatively long cephalic setae, a pear-shaped terminal pharyngeal bulb occupying less than 30% of pharyngeal length, and an elongate conical tail. the new species is distinguished from all related species by the unique characte ...201627470838
a revision of the genus macrolygistopterus pic, 1929 (coleoptera, lycidae, calochromini).a taxonomic review of the lycid genus macrolygistopterus pic, 1929 with illustrations of diagnostic characters, geographic distribution maps and an identification key to the species is presented. also, a key to the world calochromini is given. of the 12 species of the genus, 9 were studied: m. succinctus (latreille, 1811), m. quadricostatus (buquet, 1842), m. caeruleus (gorham, 1884), m. germaini pic, 1930, m. grandjeani pic, 1930, m. subparallelus pic, 1930, m. testaceirostris pic, 1930, m. sim ...201627394782
hyphomonas pacifica sp. nov., isolated from deep sea of the pacific ocean.three gram-negative, aerobic, non-spore-forming, oval- to pear-shaped bacterial strains (t16b2(t), t24b3, and c76ad) were isolated from petroleum-degrading microbial communities through an enrichment of sediments and seawater samples from the pacific ocean. phylogenetic analysis showed strains t16b2(t), t24b3, and c76ad to form a robust clade together with hyphomonas atlanticus 22ii1-22f38(t) and hyphomonas beringensis 25b14_1(t) (16s rrna identity ≥99.6 %). genomic average nucleotide identity a ...201627255138
botrytis pyriformis sp. nov., a novel and likely saprophytic species of botrytis.a novel species of botrytis from sedum sarmentosum was described based on morphology and analyses of dna sequences of nuc rdna its regions and three nuclear genes (g3pdh, hsp60, rpb2). meanwhile pathogenicity in 32 plant species, response to temperature for growth and conidial germination for the species were determined. the botrytis species was named botrytis pyriformis sp. nov. it was characterized by formation of grayish mycelia, brownish conidia and melanized sclerotia on pda. the conidia ar ...201627153884
complex processing of prickly pear cactus (opuntia sp.) by free-ranging long-tailed macaques: preliminary analysis for hierarchical organisation.complex food-processing techniques by gorillas, chimpanzees, and orangutans have allowed comparisons of complex hierarchical cognition between great apes and humans. here, we analyse preliminary observations of free-ranging long-tailed macaques (macaca fascicularis) (n = 3) in thailand processing opuntia sp. cactus fruits. from our observations, we suggest that there is potential to extend the analyses of hierarchical cognition to old world monkeys. we found that the macaques used six behavioura ...201626921023
identification and characterization of endophytic bacteria isolated from in vitro cultures of peach and pear rootstocks.endophytes are microorganisms which live symbiotically with almost all varieties of plant and in turn helping the plant in a number of ways. instead of satisfactory surface sterilization approaches, repeatedly occurring bacterial growth on in vitro rootstock cultures of peach and pear was identified and isolated as endophytic bacteria in our present study. five different isolates from peach rootstocks were molecularly identified by 16s rrna gene sequencing as brevundimonas diminuta, leifsonia sh ...201628330195
interaction of antifungal peptide bp15 with stemphylium vesicarium, the causal agent of brown spot of pear.peptide bp15 has shown antifungal activity against several plant pathogenic fungi, including stemphylium vesicarium, the causal agent of brown spot of pear. bp15 inhibits the germination, growth and sporulation of s. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. in this work, live-cell imaging was undertaken to understand the antifungal mechanism of bp15. a double-staining method based on the combination of calcofluor white and sytox green coupled w ...201626693685
expression of biphenyl synthase genes and formation of phytoalexin compounds in three fire blight-infected pyrus communis cultivars.pear (pyrus communis) is an economically important fruit crop. drops in yield and even losses of whole plantations are caused by diseases, most importantly fire blight which is triggered by the bacterial pathogen erwinia amylovora. in response to the infection, biphenyls and dibenzofurans are formed as phytoalexins, biosynthesis of which is initiated by biphenyl synthase (bis). two pcbis transcripts were cloned from fire blight-infected leaves and the encoded enzymes were characterized regarding ...201627410389
genome mapping of postzygotic hybrid necrosis in an interspecific pear population.deleterious epistatic interactions in plant inter- and intraspecific hybrids can cause a phenomenon known as hybrid necrosis, characterized by a typical seedling phenotype whose main distinguishing features are dwarfism, tissue necrosis and in some cases lethality. identification of the chromosome regions associated with this type of incompatibility is important not only to increase our understanding of the evolutionary diversification that led to speciation but also for breeding purposes. devel ...201626770810
hrcu and hrpp are pathogenicity factors in the fire blight pathogen erwinia amylovora required for the type iii secretion of dspa/e.many gram-negative bacterial pathogens mediate host-microbe interactions via utilization of the type iii secretion (t3s) system. the t3s system is a complex molecular machine consisting of more than 20 proteins. collectively, these proteins translocate effectors across extracellular space and into the host cytoplasm. successful translocation requires timely synthesis and allocation of both structural and secreted t3s proteins. based on amino acid conservation in animal pathogenic bacteria, hrcu ...201627206522
identification and expression analysis of genes related to calyx persistence in korla fragrant pear.the objective of this study was to increase understanding about genetic mechanisms affecting calyx persistence in korla fragrant pear (pyrus brestschneideri rehd). flowers were collected at early bloom, full bloom, and late bloom. the rna was extracted from the flowers and then combined according to calyx type. transcriptome and digital gene expression (dge) profiles of flowers, ovaries, and sepals with persistent calyx (sc_hua, sc_ep, and sc_zf, respectively) were compared with those of flowers ...201626911295
the rna-binding protein csra plays a central role in positively regulating virulence factors in erwinia amylovora.the gacs/gaca two-component system (also called grrs/grra) is a global regulatory system which is highly conserved among gamma-proteobacteria. this system positively regulates non-coding small regulatory rna csrb, which in turn binds to the rna-binding protein csra. however, how gacs/gaca-csr system regulates virulence traits in e. amylovora remains unknown. results from mutant characterization showed that the csrb mutant was hypermotile, produced higher amount of exopolysaccharide amylovoran, a ...201627845410
fuerstia marisgermanicae gen. nov., sp. nov., an unusual member of the phylum planctomycetes from the german wadden sea.members of the phylum planctomycetes are ubiquitous bacteria that dwell in aquatic and terrestrial habitats. while planctomycetal species are important players in the global carbon and nitrogen cycle, this phylum is still undersampled and only few genome sequences are available. here we describe strain nh11(t), a novel planctomycete obtained from a crustacean shell (wadden sea, germany). the phylogenetically closest related cultivated species is gimesia maris, sharing only 87% 16s rrna sequence ...201628066393
proteomic and ultrastructural analysis of the effect of a new nitazoxanide-n-methyl-1h-benzimidazole hybrid against giardia intestinalis.in an effort to develop alternative drugs for the treatment of giardiasis our research group has synthesized and evaluated a novel nitazoxanide and n-methyl-1h-benzimidazole hybrid molecule, named cmc-20. it showed an ic50 of 0.010 μm on giardia intestinalis, lower than the ic50 values of 0.015, 0.037 and 1.224 μm for nitazoxanide, albendazole and metronidazole, respectively. in addition, we report studies carried out on its mechanism of action and effect at the ultrastructural level on g. intes ...201627033928
lactic acid fermentation of cactus cladodes (opuntia ficus-indica l.) generates flavonoid derivatives with antioxidant and anti-inflammatory properties.cactus pear (opuntia ficus-indica l.) is widely distributed in the arid and semi-arid regions throughout the world. in the last decades, the interest towards vegetative crop increased, and cladodes are exploited for nutraceutical and health-promoting properties. this study aimed at investigating the capacity of selected lactic acid bacteria to increase the antioxidant and anti-inflammatory properties of cactus cladodes pulp, with the perspective of producing a functional ingredient, dietary supp ...201627023062
exploitation of leuconostoc mesenteroides strains to improve shelf life, rheological, sensory and functional features of prickly pear (opuntia ficus-indica l.) fruit puree.strains of leuconostoc mesenteroides were identified from raw prickly pear (opuntia ficus-indica l.). five autochthonous strains were selected based on the kinetics of growth and acidification on prickly pear fruit juice, and the capacity to synthesize exo-polysaccharides. all selected leuc. mesenteroides strains showed an in vitro mucilage-degrading capability. a protocol for processing and storage of fermented prickly pear fruit puree (fp) was set up. unstarted fp and chemically acidified fp w ...201627375258
effect of ultrasound on survival and growth of escherichia coli in cactus pear juice during storage.the aim of this study was to investigate the effectiveness of ultrasound as a conservation method for the inactivation of escherichia coli inoculated into cactus pear juices (green and purple). total soluble solids, ph, titratable acidity, and the kinetics of e. coli in cactus pear juices treated by ultrasound (60%, 70%, 80% and 90% amplitude levels for 1, 3 and 5min) were evaluated over 5 days. total inactivation was observed in both fruit juices after 5min of ultrasound treatment at most ampli ...201626991288
the impact of a cold chain break on the survival of salmonella enterica and listeria monocytogenes on minimally processed 'conference' pears during their shelf life.in recent years, improved detection methods and increased fresh-cut processing of produce have led to an increased number of outbreaks associated with fresh fruits and vegetables. during fruit and vegetable processing, natural protective barriers are removed and tissues are cut, causing nutrient rich exudates and providing attachment sites for microbes. consequently, fresh-cut produce is more susceptible to microbial proliferation than whole produce.201627813092
molecular cloning and functional analysis of a uv-b photoreceptor gene, mduvr8 (uv resistance locus 8), from apple.uvr8 (uv resistance locus 8) is an ultraviolet-b (uv-b; 280-315nm) light receptor that is involved in regulating many aspects of plant growth and development. uv-b irradiation can increase the development of flower and fruit coloration in many fruit trees, such as grape, pear and apple. previous investigations of the structure and functions of uvr8 in plants have largely focused on arabidopsis. here, we isolated the uvr8 gene from apple (malus domestica) and analyzed its function in transgenic a ...201627095405
gene characterization and transcription analysis of two new ammonium transporters in pear rootstock (pyrus betulaefolia).ammonium is the primarily nitrogen source for plant growth, but the molecular basis of ammonium acquisition in fruit species remains poorly understood. in this study, we report on the characterization of two new ammonium transporters (amt) in the perennial tree pyrus betulaefolia. in silico analyses and yeast complementation assays revealed that both pbamt1;3 and pbamt1;5 can be classified in the amt1 sub-family. the specific expression of pbamt1;3 in roots and of pbamt1;5 in leaves indicates th ...201626943161
two myb transcription factors regulate flavonoid biosynthesis in pear fruit (pyrus bretschneideri rehd.).flavonoid compounds play important roles in the modern diet, and pear fruits are an excellent dietary source of these metabolites. however, information on the regulatory network of flavonoid biosynthesis in pear fruits is rare. in this work, 18 putative flavonoid-related myb transcription factors (tfs) were screened by phylogenetic analysis and four of them were correlated with flavonoid biosynthesis patterns in pear fruits. among these myb-like genes, the specific functions of two novel myb tfs ...201626687179
dormancy-associated mads-box genes and micrornas jointly control dormancy transition in pear (pyrus pyrifolia white pear group) flower bud.bud dormancy in perennial plants is indispensable to survival over winter and to regrowth and development in the following year. however, the molecular pathways of endo-dormancy induction, maintenance, and release are still unclear, especially in fruit crops. to identify genes with roles in regulating endo-dormancy, 30 mikc(c)-type mads-box genes were identified in the pear genome and characterized. the 30 genes were analysed to determine their phylogenetic relationships with homologous genes, g ...201626466664
integration host factor is required for rpon-dependent hrpl gene expression and controls motility by positively regulating rsmb srna in erwinia amylovora.erwinia amylovora requires an hrp-type iii secretion system (t3ss) to cause disease. it has been reported that hrpl, the master regulator of t3ss, is transcriptionally regulated by sigma factor 54 (rpon), yhbh, and hrps. in this study, the role of integration host factor (ihf) in regulating hrpl and t3ss gene expression was investigated. ihf is a nucleoid-associated protein that regulates gene expression by influencing nucleoid structure and dna bending. our results showed that both ihfa and ihf ...201626368515
characterization and 1.57 å resolution structure of the key fire blight phosphatase amsi from erwinia amylovora.amsi is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the gram-negative bacterium erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. amylovoran is an exopolysaccharide that is necessary for successful infection. in order to shed light on amsi, its structure was solved at 1.57 å resolution at the same ph as its highest measured activity (ph 5.5). in the active site, a water molecule, bridging between the ...201627917839
transcriptional response of erwinia amylovora to copper shock: in vivo role of the copa gene.fire blight is a devastating plant disease caused by the bacterium erwinia amylovora, and its control is frequently based on the use of copper-based compounds whose mechanisms of action are not well known. consequently, in this article, we investigate the response of e. amylovora to copper shock by a whole-genome microarray approach. transcriptional analyses showed that, in the presence of copper, 23 genes were increased in expression; these genes were classified mainly into the transport and st ...201627862834
the in planta proteome of wild type strains of the fire blight pathogen, erwinia amylovora.erwinia amylovora is a gram-negative plant pathogen that causes fire blight. this disease affects most members of the rosaceae family including apple and pear. here, an infection model is introduced to study proteomic changes in a highly virulent e. amylovora strain upon interaction with its host as compared to a lower virulent strain. for this purpose separate shoots of apple rootstocks were wound-infected and when infection became systemic, bacterial cells were isolated and processed for analy ...201626924300
phylogenetic analysis of pr genes in some pome fruit species with the emphasis on transcriptional analysis and ros response under erwinia amylovora inoculation in apple.attempts were made to identify eight pathogenesis related (pr) genes (i.e., pr-1a, pr3-ch1, pr3-ch2, pr3-ch3, pr3-ch4, pr3-ch5, pr-5 and pr-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen erwinia amylovora, the causal agent of fire blight. totally, 32 pr genes of different families were obtained, excepting pr3-ch2 (amplified only in apple) and pr3-ch4 (amplified only in apple and pear), the others were successfully amplified in all t ...201626589211
dickeyafangzhongdai sp. nov., a plant-pathogenic bacterium isolated from pear trees (pyrus pyrifolia).gram-stain-negative, pectinolytic bacteria were repeatedly isolated from pear trees displaying symptoms of bleeding canker in china. three strains, js5t, ln1 and qzh3, had identical 16s rrna gene sequences that shared 99 % similarity to the type strain of dickeya dadantii. phylogenetic analysis of strains js5t, ln1 and qzh3 with isolates representing all species of the genus dickeya and related pectobacterium species supported their affiliation to dickeya. multi-locus sequence typing employing c ...201627045848
survival and growth of cronobacter sakazakii on fresh-cut fruit and the effect of uv-c illumination and electrolyzed water in the reduction of its population.cronobacter sakazakii, found in foods such as powdered infant formula and plant origin ready-to-eat food, is an opportunistic pathogen to infants, neonates and vulnerable adults. the objective of this study was to monitor the growth of c. sakazakii in fresh-cut 'royal gala' apple, 'rocha' pear, and 'piel de sapo' melon, and the effect of uv-c illumination, acidic electrolyzed water (aew) and neutral electrolyzed water (new) in the reduction of its population. fresh-cut fruits were inoculated and ...201627163653
elucidation of enterotoxigenic bacillus cereus outbreaks in austria by complementary epidemiological and microbiological investigations, 2013.identifying bacillus cereus as the causative agent of a foodborne outbreak still poses a challenge. we report on the epidemiological and microbiological investigation of three outbreaks of food poisoning (a, b, and c) in austria in 2013. a total of 44% among 32 hotel guests (a), 22% among 63 employees (b) and 29% among 362 residents of a rehab clinic (c) fell sick immediately after meal consumption. b. cereus isolated from left overs or retained samples from related foods were characterized by t ...201627257745
characterization of virus-derived small interfering rnas in apple stem grooving virus-infected in vitro-cultured pyrus pyrifolia shoot tips in response to high temperature treatment.heat treatment (known as thermotherapy) together with in vitro culture of shoot meristem tips is a commonly used technology to obtain virus-free germplasm for the effective control of virus diseases in fruit trees. rna silencing as an antiviral defense mechanism has been implicated in this process. to understand if high temperature-mediated acceleration of the host antiviral gene silencing system in the meristem tip facilitates virus-derived small interfering rnas (vsirna) accumulation to reduce ...201627716257
integrated analyses using rna-seq data reveal viral genomes, single nucleotide variations, the phylogenetic relationship, and recombination for apple stem grooving virus.next-generation sequencing (ngs) provides many possibilities for plant virology research. in this study, we performed integrated analyses using plant transcriptome data for plant virus identification using apple stem grooving virus (asgv) as an exemplar virus. we used 15 publicly available transcriptome libraries from three different studies, two mrna-seq studies and a small rna-seq study.201627507588
genome sequences of three apple chlorotic leaf spot virus isolates from hawthorns in china.the genome sequences of apple chlorotic leaf spot virus (aclsv) isolates from three accessions of hawthorns (crataegus pinnatifida) grown at shenyang agricultural university were determined using illumina rna-seq. to confirm the assembly data from the de novo sequencing, two aclsv genomic sequences (sy01 and sy02) were sequenced using the sanger method. the sy01 and sy02 sequences obtained with the sanger method showed 99.5% and 99.7% nucleotide identity with the transcriptome data, respectively ...201627519059
physical mapping of black spot disease resistance/susceptibility-related genome regions in japanese pear (pyrus pyrifolia) by bac-fish.black spot disease, caused by alternaria alternata japanese pear pathotype, is one of the most harmful diseases in japanese pear cultivation. in the present study, the locations of black spot disease resistance/susceptibility-related genome regions were studied by fluorescence in situ hybridization using bac clone (bac-fish) on japanese pear (pyrus pyrifolia (burm. f.) nakai) chromosomes. root tips of self-pollinated seedlings of 'osa gold' were used as materials. chromosome samples were prepare ...201627436955
characterization and comparison of the cpk gene family in the apple (malus × domestica) and other rosaceae species and its response to alternaria alternata infection.as one of the ca2+ sensors, calcium-dependent protein kinase (cpk) plays vital roles in immune and stress signaling, growth and development, and hormone responses, etc. recently, the whole genome of apple (malus × domestica), pear (pyrus communis), peach (prunus persica), plum (prunus mume) and strawberry (fragaria vesca) in rosaceae family has been fully sequenced. however, little is known about the cpk gene family in these rosaceae species. in this study, 123 cpk genes were identified from fiv ...201627186637
fine mapping of the gene for susceptibility to black spot disease in japanese pear (pyrus pyrifolia nakai).black spot disease, which is caused by the japanese pear pathotype of the filamentous fungus alternaria alternata (fries) keissler, is one of the most harmful diseases in japanese pear cultivation. we mapped a gene for susceptibility to black spot disease in the japanese pear (pyrus pyrifolia nakai) cultivar 'kinchaku' (aki gene) at the top of linkage group 11, similar to the positions of the susceptibility genes ani in 'osa nijisseiki' and ana in 'nansui'. using synteny-based marker enrichment, ...201627162498
characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree.apple stem grooving virus (asgv), apple chlorotic leaf spot virus (aclsv), and prunus necrotic ringspot virus (pnrsv) were identified in a crab apple tree by small rna deep sequencing. the complete genome sequence of aclsv isolate bj (aclsv-bj) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other aclsv isolates. a phylogenetic tree based on the complete genome sequence of all available aclsv isolates showed that aclsv-bj clustered with the isolates sy01 from hawtho ...201727990565
the prevalence and impact of babesia canis and theileria sp. in free-ranging grey wolf (canis lupus) populations in croatia.babesia spp. and theileria spp. are important emerging causes of disease in dogs. alongside these domesticated hosts, there is increasing recognition that these piroplasms can also be found in a range of wild animals with isolated reports describing the presence of these pathogen in foxes (vulpes vulpes) and captive grey wolves (canis lupus). the prevalence and impact of these infections in free-ranging populations of canids are unknown. to gain a better insight into the epidemiology and pathoge ...201728376903
fermentation of dietetic fiber from green bean and prickly pear shell by pure and mixture culture of lactobacillus acidophilus la-5 and bifidobacterium bifidum 450b.the aim of this study was to evaluate the fermentation of dietary fiber from green bean (phaseolus vulgaris) and prickly pear shell (opuntia ficus-indica) by lactobacillus acidophilus la-5 and bifidobacterium bifidum 450b growing as mono-culture and co-culture, the fermentation products, and proteins expressed during this process. the analysis of the fermentation profile showed a major growth of bacteria in the culture media of each dietary fiber supplemented with glucose, and particularly b. bi ...201728332163
efficient expression of an acidic endo-polygalacturonase from aspergillus niger and its application in juice production.an endo-polygalacturonase gene (pga-zj5a) was cloned by reverse transcription from cdnas synthesized from aspergillus niger zj5 total rna. the open reading frame of pga-zj5a was 1089 base pairs encoding 362 amino acids. pga-zj5a lacking a signal peptide sequence was successfully amplified using a. niger zj5 cdna as the template and was ligated into the ppic9 vector. the resulting plasmid was transformed into competent cells of pichia pastoris gs115 for heterologous expression. the polygalacturon ...201728248099
chemical variability, antioxidant and antifungal activities of essential oils and hydrosol extract of calendula arvensis l. from western algeria.the chemical composition of the essential oils and hydrosol extract from aerial parts of calendula arvensis l. was investigated using gc-fid and gc/ms. intra-species variations of the chemical compositions of essential oils from 18 algerian sample locations were investigated using statistical analysis. chemical analysis allowed the identification of fifty-three compounds amounting to 92.3-98.5% with yields varied of 0.09-0.36% and the main compounds were zingiberenol 1 (8.7-29.8%), eremoligenol ...201728109063
whole-genome sequence of pseudomonas fluorescens ek007-rg4, a promising biocontrol agent against a broad range of bacteria, including the fire blight bacterium erwinia amylovora.here, we report the first draft whole-genome sequence of pseudomonas fluorescens strain ek007-rg4, which was isolated from the phylloplane of a pear tree. p. fluorescens ek007-rg4 displays strong antagonism against erwinia amylovora, the causal agent for fire blight disease, in addition to several other pathogenic and non-pathogenic bacteria.201728360179
evaluation of food lures for capture and monitoring of anastrepha fraterculus (diptera: tephritidae) on temperate fruit trees.the anastrepha fraterculus (wiedemann) (diptera: tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of brazil. the objective of this work was to evaluate the efficiency of the major commercial food lures used in brazil for trapping and monitoring of a. fraterculus in plum, pear, and feijoa orchards. the assessed lures were hydrolyzed proteins of animal origin (ceratrap) and plant origin (bioanastrepha), torula yeast + borax (torula), and grape juice. ...201728334322
transcriptome analysis of bagging-treated red chinese sand pear peels reveals light-responsive pathway functions in anthocyanin accumulation.bagging is an efficient method to improve fruit colour development. this work reported a transcriptome analysis using bagging-treated red chinese sand pear peels. in total, 8,870 differentially expressed genes were further analysed by a weighted gene co-expression network analysis and early-, middle- and late light-responsive genes were identified. an annotation analysis revealed several pathways involved in the different responsive stages. the presence of long hypocotly 5, cry-dash and a consta ...201728246400
extracts from two ubiquitous mediterranean plants ameliorate cellular and animal models of neurodegenerative proteinopathies.a signature feature of age-related neurodegenerative proteinopathies is the misfolding and aggregation of proteins, typically amyloid-β (aβ) in alzheimer's disease (ad) and α-synuclein (α-syn) in parkinson's disease (pd), into soluble oligomeric structures that are highly neurotoxic. cellular and animal models that faithfully replicate the hallmark features of these disorders are being increasing exploited to identify disease-modifying compounds. natural compounds have been identified as a usefu ...201727919712
viable bacterial population and persistence of foodborne pathogens on the pear carpoplane.knowledge on the culturable bacteria and foodborne pathogen presence on pears is important for understanding the impact of postharvest practices on food safety assurance. pear fruit bacteria were investigated from the point of harvest, following chlorine drenching and after controlled atmosphere (ca) storage to assess the impact on natural bacterial populations and potential foodborne pathogens.201727300139
pcppi: a comprehensive database for the prediction of penicillium-crop protein-protein interactions.penicillium expansum , the causal agent of blue mold, is one of the most prevalent post-harvest pathogens, infecting a wide range of crops after harvest. in response, crops have evolved various defense systems to protect themselves against this and other pathogens. penicillium -crop interaction is a multifaceted process and mediated by pathogen- and host-derived proteins. identification and characterization of the inter-species protein-protein interactions (ppis) are fundamental to elucidating t ...201728365721
comparison of the effects of three types of aminobutyric acids on the control of penicillium expansum infection in pear fruit.aminobutyric acids were reported to have inhibitory effects on postharvest fungal diseases. the effects of α-aminobutyric acid (aaba), β-aminobutyric acid (baba) and γ-aminobutyric acid (gaba) on the control of penicillium expansum infection in pear fruit were compared in the present study.201727392194
molecular cloning and heterologous expression analysis of jrvte1 gene from walnut (juglans regia).tocopherol cyclase (vte1) plays a key role in promoting the production of γ-tocopherol and improving total tocopherol content in photosynthetic organisms. walnut is an important source of tocopherols in the human diet, and γ-tocopherol is the major tocopherol compound in walnut kernels. in this study, a full-length cdna of the vte1 gene was isolated from walnut using rt-pcr and race, and designated as jrvte1. the full-length cdna of the jrvte1 gene contained a 1353-bp open-reading frame encoding ...201726612974
exposure to minimally processed pear and melon during shelf life could modify the pathogenic potential of listeria monocytogenes.survival and virulence of foodborne pathogens can be influenced by environmental factors such as the intrinsic properties of food as well as the extrinsic properties that contribute to food shelf life (e.g., temperature and gas atmosphere). the direct contribution of food matrix characteristics on the survival of l. monocytogenes during fresh-cut fruit shelf life is not very well understood. in addition, the gastrointestinal tract is the primary route of listeriosis infection and penetration of ...201727889159
comparative transcriptomic analysis reveals that ethylene/h2o2-mediated hypersensitive response and programmed cell death determine the compatible interaction of sand pear and alternaria alternata.a major restriction on sand pear (pyrus pyrifolia) production is black spot disease caused by the necrotrophic fungus alternaria alternata. however, the pear response mechanism to a. alternata is unknown at the molecular level. here, host responses of a resistant cultivar cuiguan (cg) and a susceptible cultivar sucui1 (sc1) to a. alternata infection were investigated. we found that the primary necrotic lesion formed at 1 dpi and the expansion of lesions was aggressive in sc1. data from transcrip ...201728261248
patterns of low temperature induced accumulation of dehydrins in rosaceae crops-evidence for post-translational modification in apple.important crop plants of rosaceae family are often damaged during winter due to the lack of acclimation and cold hardiness. one of the cellular responses of plants to cold stress is the accumulation of dehydrin proteins. we studied the expression of dehydrins in several rosaceae species during low temperature treatment in vitro. microshoots of pyrus communis, malus×domestica, fragaria vesca, fragaria×ananassa, prunus cerasus and prunus avium cultivars were grown in low temperature conditions. ge ...201728886453
integrated high-density consensus genetic map of pyrus and anchoring of the 'bartlett' v1.0 (pyrus communis) genome.genetic maps are essential tools for pear genetics and genomics research. in this study, we first constructed an integrated simple sequence repeat (ssr) and single nucleotide polymorphism (snp)-based consensus genetic map for pear based on common ssr markers between nine published maps. a total of 5,085 markers, including 1,232 ssrs and 3,853 snps, were localized on a consensus map spanning 3,266.0 cm in total, with an average marker interval of 0.64 cm, which represents the highest density cons ...201728130382
comparative analysis of the predicted secretomes of rosaceae scab pathogens venturia inaequalis and v. pirina reveals expanded effector families and putative determinants of host range.fungal plant pathogens belonging to the genus venturia cause damaging scab diseases of members of the rosaceae. in terms of economic impact, the most important of these are v. inaequalis, which infects apple, and v. pirina, which is a pathogen of european pear. given that venturia fungi colonise the sub-cuticular space without penetrating plant cells, it is assumed that effectors that contribute to virulence and determination of host range will be secreted into this plant-pathogen interface. thu ...201728464870
gorham-stout disease management during pregnancy.gorham-stout disease (gsd) is a rare lymphatic disorder affecting children or young adults with no predilection of sex. it is generally associated with vanishing bone osteolytic lesions, thoracic and abdominal involvement, and diffuse pulmonary lymphangiomatosis. chylous effusions and chylothorax, consequent to the abnormal proliferation of lymphatic vessels, may induce respiratory failure with a high mortality risk. extrapulmonary alterations may include chylous ascites, lymphopenia, and destru ...201729263942
two cases of gorham-stout disease with good response to zoledronic acid treatment.gorham-stout disease - also called vanishing bone syndrome - is a rare bone disease characterized by a progressive intra-osseous proliferation of non-neoplastic vascular tissue resulting in massive osteolysis. here, we report two clinical cases of gorham-stout disease. case 1: a 56-year-old woman with 20 years of history of pain and swell in elbows, ankles and wrist. then she was diagnosed as systemic lupus erythematosus (sle) with glomerulonephritis type iii. after other pathologies were ruled ...201729263745
systematics of the east palaearctic pear psyllids (hemiptera: psylloidea) with particular focus on the japanese and korean fauna.the confused taxonomy of the east palaearctic pear psyllids, serious pests on cultivated pear, is reviewed. fifty-six nominal species have been reported from pyrus, 25 of which we consider valid and ten as not being associated with pyrus. our taxonomic revision suggests that, in korea, four cacopsylla species develop on pear: the univoltine c. burckhardti luo et al. previously misidentified as c. pyrisuga (foerster), the polyvoltine, seasonally dimorphic c. jukyungi (kwon) (winter form 'cinereos ...201729245444
improvement of post-harvest quality of pear fruit with optimized composite edible coating formulations.application of edible coatings is a suitable method to maintain the quality and reduce post-harvest losses in fresh vegetables and fruits. pear fruits being climacteric have a short shelf life, and coating is considered as one of the most popular techniques to prolong its shelf life.the present study evaluates the effect of optimized edible coatings containing soy protein isolate (spi) in combination with additives like hydroxypropyl methylcellulose (hpmc) and olive oil on 'babughosha' pears (py ...201729085134
mandibular gorham-stout disease: a case report and literature review.gorham-stout disease (gsd) is characterized by aggressive bone resorption, proliferation of vascular or lymphatic vessels, and soft-tissue swelling. bones that initially appear normal start to resorb, partially or completely. however, the etiology of gsd is unknown.201729049202
vanishing act: gorham-stout disease leading to dynamic cerebrospinal fluid abnormalities. 201729016411
biology and epidemiology of venturia species affecting fruit crops: a review.the fungal genus venturia sacc. (anamorph fusicladium bonord.) includes plant pathogens that cause substantial economic damage to fruit crops worldwide. although venturia inaequalis is considered a model species in plant pathology, other venturia spp. also cause scab on other fruit trees. relative to the substantial research that has been conducted on v. inaequalis and apple scab, little research has been conducted on venturia spp. affecting other fruit trees. in this review, the main characteri ...201728974954
langerhans cell histiocytosis involving both jaws in an adult.langerhans cell histiocytosis (lch) is the latest terminology for a disorder of reticulo-endothelial system, previously known as histiocytosis x, and marked by aberrant proliferation of bone marrow derived langerhans cells with variable inflammatory infiltrate including neutrophils, lymphocytes, plasma cells, eosinophils, and multinucleated giant cells. although rare, the disorder frequently inflicts children with peak incidence recorded in 2-4 years age group. lch is rare in adults. a22-year ad ...201728969735
grafting on resistant interstocks reduces scion susceptibility to pear psylla, cacopsylla bidens.pear psylla is a major obstacle to efficient integrated pest management in pear orchards in israel and around the world. we used two accessions with natural resistance to pear psylla cacopsylla bidens (šulc) - py.760-261 (760) and py.701-202 (701), both apparently of pyrus communis l. origin - as interstock grafts to confer psylla resistance to the commercially important 'spadona estiva' (pyrus communis) scion (spadona) cultivar. the interaction of the interstocks with quince (cydonia oblong mil ...201728967187
gorham-stout disease: hand involvement in an 8-year-old child. 201728964427
generation of an infectious clone of amdv and identification of capsid residues essential for infectivity in cell culture.pathogenic strains of aleutian mink disease virus (amdv) such as utah-1 do not replicate in cell culture (e.g., crandell rees feline kidney cells) while the in vitro-adapted amdv strain adv-gorham (adv-g) is not pathogenic. here, we constructed a full-length infectious clone (padv-g). alignment of the vp2 gene of adv-g with that of other amdv strains revealed many amino acid (a.a.) residues conserved among pathogenic isolates that differed in adv-g. four virulence-associated, conserved residues ...201728923508
population growth of dysaphis pyri (hemiptera: aphididae) on different pear cultivars with discussion on curve fitting in life table studies.population growth parameters of the dysaphis pyri (boyer de fonscolombe) (hemiptera: aphididae) were evaluated on four different cultivars (coscia, ankara, williams, and santa-maria) of pear (pyrus communis l.) under field conditions in the van region of turkey. aphids were kept on leaves of 10-yr-old pear trees in plexiglas clip-cells (20 mm in diameter and 10 mm in height, with the upper side covered with muslin). for the description of the stage differentiation during population growth, we an ...201728854662
gorham-stout disease presenting as acute unilateral proptosis.gorham-stout disease (gsd) is a rare angiomatous disorder characterized by osteolysis. we report the case of a 66-year-old woman who developed sudden left eye proptosis and facial pain. neuroimaging demonstrated a destructive mass involving the roof and lateral wall of the left orbit with adjacent abnormal orbital soft tissue. an initial biopsy of the soft tissue was nondiagnostic; however, transcranial biopsy of the orbital roof revealed an intraosseous cavernous angioma, with infiltration of o ...201728837441
gorham-stout disease affecting the mandible: bone scintigraphy and computed tomography findings.we present images of a 13-year-old boy with gorham-stout disease affecting the mandible. initial bone scintigraphy showed poor visualization of tc-methylene diphosphonate (tc-mdp) uptake in the mandible body. however, abnormally increased tc-mdp uptake was noted in the bilateral mandibular rami. in the follow-up examination a year later, bone scintigraphy indicated that the disease had progressed to the bilateral mandibular rami with no uptake. in addition, massive osteolysis of the mandible was ...201728806245
mr imaging findings of vertebral involvement in gorham-stout disease, generalized lymphatic anomaly, and kaposiform lymphangiomatosis.to assess the mr imaging findings of vertebral involvement in gorham-stout disease (gsd), generalized lymphatic anomaly (gla), and kaposiform lymphangiomatosis (kla).201728795277
systematics of the ceracis furcifer species-group (coleoptera: ciidae): the specialized consumers of the blood-red bracket fungus pycnoporus sanguineus.the ceracis furcifer species-group (coleoptera: ciidae) originally comprised nine species names: ceracis cornifer (mellié, 1849); c. cylindricus (brèthes, 1922); c. furcifer mellié, 1849; c. hastifer (mellié, 1849); c. monocerus lawrence, 1967; c. ruficornis pic, 1916; c. simplicicornis (pic, 1916); c. semipallidus pic, 1922 and c. unicornis gorham, 1898. ceracis semipallidus was synonymised with c. furcifer and then no further changes were made to the composition of the group. here, we provide ...201728714939
a rare case of chylothorax. gorham-stout syndrome. 201728705386
a practical examination of rna isolation methods for european pear (pyrus communis).with the goal of identifying fast, reliable, and broadly applicable rna isolation methods in european pear fruit for downstream transcriptome analysis, we evaluated several commercially available kit-based rna isolation methods, plus our modified version of a published cetyl trimethyl ammonium bromide (ctab)-based method.201728662720
'bartlett' pear fruit (pyrus communis l.) ripening regulation by low temperatures involves genes associated with jasmonic acid, cold response, and transcription factors.low temperature (lt) treatments enhance ethylene production and ripening rate in the european pear (pyrus communis l.). however, the underlying molecular mechanisms are not well understood. this study aims to identify genes responsible for ripening enhancement by lt. to this end, the transcriptome of 'bartlett' pears treated with lt (0°c or 10°c for up to 14 d), which results in faster ripening, and control pears without conditioning treatment was analyzed. lt conditioned pears reached eating fi ...201728554478
low molecular weight chitosan is an effective antifungal agent against botryosphaeria sp. and preservative agent for pear (pyrus) fruits.antifungal activity and preservative effect of a low molecular weight chitosan (lmwc) sample, derived from chitosan by enzymatic hydrolysis, were investigated in vitro and in vivo. a pathogenic fungal strain was isolated from decayed pear (pyrus bretschneideri cv. "huangguan") fruit and identified as botryosphaeria sp. w-01. lmwc was shown to strongly inhibit w-01 growth based on studies of minimum inhibitory concentration (mic) and effects on mycelial biomass and radial growth of the fungus. lm ...201727818296
<i>neolinoptes</i> gen. n., a replacement name for the net-winged beetle genus <i>linoptes</i> gorham, 1884 and a new species of <i>lycomorphon</i> from guyana (coleoptera: lycidae).neolinoptes gen. n. is erected to replace linoptes gorham, 1884, preoccupied by linoptes menge, 1854 (arachnida: araneae). consequently, neolinoptes imbrex (gorham, 1884) comb. n., n. amazonicus (pic, 1923) comb. n., n. atronotatus (pic, 1922) comb. n., n. atripennis (pic, 1932) comb. n. are proposed. calocladon rubidum gorham, 1884 is transferred to neolinoptes. lycomorphon iwokrama sp. n. is proposed as new to science and the genus is recorded from guyana for the first time. additionally, fals ...201728183117
validation of daily increment deposition and early growth of mud carp cirrhinus molitorella.in this study, laboratory reared cirrhinus molitorella from hatching through to the juvenile stage were used to validate daily increment deposition. lapilli were suitable for ageing c. molitorella. the slope of the linear relationships between increment counts and age was not significantly different from 1 (n = 258, p > 0·05), indicating that growth increments are deposited daily. the first increment following the first-feeding check formed 3 days post hatch (dph). one hundred and twenty-three j ...201728105739
parathelohania iranica sp. nov. (microsporidia: amblyosporidae) infecting malaria mosquito anopheles superpictus (diptera: culicidae): ultrastructure and molecular characterization.microsporidia are common pathogens of insects and sometimes are considered as a candidate in the biological control of mosquitoes. recently a microsporidium infection was discovered in anopheles superpictus (diptera: culicidae) larvae, in iran. the responsible agent belonged to the genus parathelohania (microsporidia: amblyosporidae). this study has been carried out to identify its identity at the species level. fresh infected larvae were collected from the type locality, kiar district, in chaha ...201728372996
influence of the fiber from agro-industrial co-products as functional food ingredient on the acceptance, neophobia and sensory characteristics of cooked sausages.the sensory analysis of new products is essential for subsequent acceptance by consumers, moreover in the functional food market. the acceptance and food neophobia of cooked sausages formulated with cactus pear fiber or pineapple pear fiber, as functional ingredient, was complemented with a sensory characterization by r-index and qualitative descriptive analysis (qda). female consumers aged between 40 and 50 years showed greater interest in the consumption of healthy foods, with a higher level o ...201728242937
candidate pheromone receptors of codling moth cydia pomonella respond to pheromones and kairomones.olfaction plays a dominant role in the mate-finding and host selection behaviours of the codling moth (cydia pomonella), an important pest of apple, pear and walnut orchards worldwide. antennal transcriptome analysis revealed a number of abundantly expressed genes related to the moth olfactory system, including those encoding the olfactory receptors (ors) cpomor1, cpomor3 and cpomor6a, which belong to the pheromone receptor (pr) lineage, and the co-receptor (cpomorco). using heterologous express ...201728117454
gorham-stout syndrome: phantom bone disease.gorham-stout syndrome is a disease that presents idiopathic osteolysis of a bone or closely contiguous area. the etiology is unknown. it is a rare condition that is difficult to diagnose, and its treatment is controversial. it affects individuals irrespective of age or sex. in this study, we conducted a bibliographic review of the disease, specifically focusing on the differential diagnosis, and we demonstrated the follow-up on a patient with this syndrome from the time of its diagnosis, through ...201727026974
[radiologic findings in gorham-stout syndrome].gorham-stout syndrome, also called "disappearing bone disease" or idiopathic massive osteolysis, is a rare disease of unknown etiology and pathogenesis. it is characterized by rapidly progressive localized massive osteolysis associated to a proliferation of vascular structures of benign origin in which the absence of new bone formation is representative. we present two cases (a six-year-old boy with affected right ulna, and a 15-year-old girl with right rib affection associated to chylothorax) o ...201717059207
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