Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| hantavirus infection of dendritic cells. | dendritic cells (dcs) play a pivotal role as antigen-presenting cells in the antiviral immune response. here we show that hantaan virus (htnv), which belongs to the bunyaviridae family (genus hantavirus) and causes hemorrhagic fever with renal syndrome, productively infects human dcs in vitro. in the course of htnv infection, dcs did not show any cytopathic effect and viral replication did not induce cell lysis or apoptosis. furthermore, htnv did not affect apoptosis-inducing signals that are im ... | 2002 | 12368315 |
| pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. | hantaviruses cause two human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hantaviruses infect human endothelial cells but cause little or no damage to the infected endothelium. we analyzed with affymetrix dna arrays (santa clara, ca) the endothelial cell transcriptional responses directed by hantaviruses associated with hps [new york-1 virus (ny-1v)], hfrs [hantaan virus (htnv)], or by a hantavirus not associated with human disease [prospect hil ... | 2002 | 12368479 |
| viral pneumonias in adults: radiologic and pathologic findings. | numerous viruses, including influenza virus, measles virus, hantavirus, adenovirus, herpesviruses, varicella-zoster virus, cytomegalovirus, and epstein-barr virus, can cause lower respiratory tract infection in adults. viral pneumonia in adults can be classified into two clinical groups: so-called atypical pneumonia in otherwise healthy hosts and viral pneumonia in immunocompromised hosts. influenza virus types a and b cause most cases of viral pneumonia in immunocompetent adults. immunocompromi ... | 2002 | 12376607 |
| pathogenic hantaviruses selectively inhibit beta3 integrin directed endothelial cell migration. | hantaviruses cause two diseases of man, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). pathogenic and non-pathogenic hantaviruses use beta3 and beta1 integrins, respectively, to enter endothelial cells. beta3 integrins were recently reported to bind receptors that regulate vascular permeability suggesting that hantavirus beta3 integrin interactions may regulate endothelial cell function and contribute to viral pathogenesis. in this study we investigated the ... | 2002 | 12376753 |
| rodent host specificity of european hantaviruses: evidence of puumala virus interspecific spillover. | in order to investigate rodent host specificity of european hantaviruses, experimental infection of colonized and wild-trapped rodents was performed. in addition to the natural rodent reservoir, clethrionomys glareolus, puumala hantavirus (puuv) could infect colonized microtus agrestis and lemmus sibiricus, but not syrian hamsters or balb/c mice. neither c. glareolus, nor m. agrestis, could be readily infected by tula hantavirus (tulv). wild-trapped apodemus flavicollis and a. agrarius, the natu ... | 2002 | 12376967 |
| hantaviruses in estonia. | human serum samples collected from healthy individuals in 14 counties were screened by elisa in order to investigate the presence of hantavirus infections in estonia. out of 1,234 serum samples, 124 were found positive for hantavirus-specific igg and were subsequently serotyped by a focus reduction neutralization test. a total of 112 samples neutralized at least one of the examined hantaviruses-puumala (puuv), saaremaa (saav), dobrava (dobv), hantaan, and seoul viruses-and thereby, the focus red ... | 2002 | 12376968 |
| serological evidence of human hantavirus infections in indonesia. | 2002 | 12382098 | |
| hantavirus pulmonary syndrome in uberaba, minas gerais, brazil. | this report describes the epidemiological and clinical-evolutive characteristics of eight patients with hantavirus pulmonary syndrome (hps) in uberaba, minas gerais, brazil. a positive history of contact with rodents was present in 100% of the cases. the time between the onset of symptoms and hospital care was, on average, 3.6 days. all patients showed clinical and laboratory findings suggestive of hps. elevated urea and creatinine levels were observed in 6 (75%) cases, po2 was < 60 mmhg in 100% ... | 2002 | 12386696 |
| exposure to rodents and rodent-borne viruses among persons with elevated occupational risk. | persons who have frequent contact with rodents as part of their occupation may be at increased risk of exposure to rodent-borne viruses such as sin nombre virus (snv), the agent of hantavirus pulmonary syndrome, and whitewater arroyo virus (wwa), a new world arenavirus. eighty-one persons with possible occupational exposure to rodents completed questionnaires and provided specimens for serologic testing. seventy-two participants reported handling rodents as part of their job. the mean total numb ... | 2002 | 12391776 |
| dinara - new natural focus of hemorrhagic fever with renal syndrome in croatia. | to investigate the characteristics and determine risk factors for hantanvirus infection in natural focus of hemorrhagic fever with renal syndrome (hfrs) on the dinara mountain, where outbreak of disease emerged among croatian soldiers in 1995, and to describe the features of hfrs acquired on the dinara mountain and determine the scale of the largest hfrs epidemic so far in croatia. | 2002 | 12402400 |
| epidemiologic characteristics and military implications of hemorrhagic fever with renal syndrome in croatia. | to analyze epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia, including military implications of the disease and measures for its prevention. | 2002 | 12402401 |
| maporal viral infection in the syrian golden hamster: a model of hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome (hps) is a severe and often fatal rodent-borne zoonosis. maporal (map) virus is a newly discovered hantavirus that originally was isolated from an arboreal rice rat captured in central venezuela. the results of this study indicate that map virus in the syrian golden hamster (mesocricetus auratus) can cause a disease that is clinically and pathologically remarkably similar to hps. the similarities include the time course of clinical disease, presence of virus-specifi ... | 2002 | 12404153 |
| novel and challenging infections of man. a brief overview. | the greatest challenge to human health of recent times is the pandemic caused by the retroviruses known as hiv1 and hiv2. these have unleashed a plethora of opportunistic infections with organisms ranging from other viruses to helminths, some of which are newly recognised taxa or organisms never before identified as serious pathogens of man. animal and bird migration as well as human travel and international trade have extended, to an alarming degree, the dissemination of other viral infections. ... | 2002 | 12404808 |
| the in vitro and in vivo protective activity of monoclonal antibodies directed against hantaan virus: potential application for immunotherapy and passive immunization. | hantaan virus (htnv), a member of the genus hantavirus, family bunyaviridae, is an etiologic agent causing a serious human disease, hemorrhagic fever with renal syndrome (hfrs), with a mortality as high as 15% and is also a potential bioterrorism agent. it is urgently needed to develop anti-htnv-neutralizing monoclonal antibodies (mabs) for treatment and prevention of htnv infection. in the present study, 18 murine mabs directed against htnv strain chen were generated and characterized. among th ... | 2002 | 12408987 |
| emerging and re-emerging infectious diseases. | in human history, numerous infectious diseases have emerged and re-emerged. aside from many others, the so-called 'exotic' agents in particular are a threat to our public health systems due to limited experience in case management and lack of appropriate resources. many of these agents are zoonotic in origin and transmitted from animals to man either directly or via vectors. the reservoirs are often infected subclinically or asymptomatically and the distribution of the diseases basically reflect ... | 2002 | 12410344 |
| elevated generation of reactive oxygen/nitrogen species in hantavirus cardiopulmonary syndrome. | hantavirus cardiopulmonary syndrome (hcps) is a life-threatening respiratory disease characterized by profound pulmonary edema and myocardial depression. most cases of hcps in north america are caused by sin nombre virus (snv), which is carried asymptomatically by deer mice (peromyscus maniculatus). the underlying pathophysiology of hcps is poorly understood. we hypothesized that pathogenic snv infection results in increased generation of reactive oxygen/nitrogen species (rons), which contribute ... | 2002 | 12134039 |
| genetic characterization of hantaviruses transmitted by the korean field mouse (apodemus peninsulae), far east russia. | in an epizootiologic survey of 122 rodents captured in vladivostok, russia, antibodies positive for hantavirus were found in apodemus peninsulae (4/70), a. agrarius (1/39), and clethrionomys rufocanus (1/8). the hantavirus sequences identified in two seropositive a. peninsulae and two patients with hemorrhagic fever with renal syndrome (hfrs) from the primorye region of far east russia were designated as solovey and primorye, respectively. the nucleotide sequences of the solovey, primorye, and a ... | 2002 | 12141960 |
| hantaan virus infection causes an acute neurological disease that is fatal in adult laboratory mice. | hantaan virus, the etiological agent of korean hemorrhagic fever, is transmitted to humans from persistently infected mice (apodemus agrarius), which serve as the primary reservoir. here we demonstrate that several strains of adult mus musculus domesticus (c57bl/6, balb/c, akr/j, and sjl/j) were susceptible to hantaan virus infection when infected intraperitoneally. first clinical signs were loss of weight, ruffled fur, and reduced activity, which were followed by neurological symptoms, such as ... | 2002 | 12163608 |
| [epidemic nephropathy. an important differential diagnosis of acute renal failure in reutlingen, an endemic area]. | hantavirus ("epidemic") nephropathy (hn), or hemorrhagic fever with renal syndrome (who nomenclature), is an infection caused by strains (e.g. puumala) of hantavirus, which is often accompanied by acute renal failure. although its typical symptomatology has been fully reported, it is rarely included in the differential diagnosis of renal nephropathy. this study investigated whether, in the presence of typical symptoms, specific search for a certain constellation of laboratory values in associati ... | 2002 | 12183800 |
| complete nucleotide sequence of the m rna segment of andes virus and analysis of the variability of the termini of the virus s, m and l rna segments. | hantavirus pulmonary syndrome (hps) has been recognized increasingly as a significant public health problem in south america since andes virus was first discovered in argentina. here, the isolation of andes virus is reported from an infected rodent captured in argentina in close vicinity to the place of the first hps case, ah1. the complete nucleotide sequences of the virus m segment, partial l segment and the termini of the s, m and l segment genome rnas were determined. the andes virus m rna s ... | 2002 | 12185264 |
| hantavirus pulmonary syndrome--united states: updated recommendations for risk reduction. centers for disease control and prevention. | this report provides updated recommendations for prevention and control of hantavirus infections associated with rodents in the united states. it supersedes the previous report (cdc. hantavirus infection--southwestern united states: interim recommendations for risk reduction. mmwr 1993; 42[no. rr-11]: 1-13). these recommendations are based on principles of rodent and infection control, and accumulating evidence that most infections result from exposure, in closed spaces, to active infestations o ... | 2002 | 12194506 |
| demographic factors associated with hantavirus infection in bank voles (clethrionomys glareolus). | the bank vole (clethrionomys glareolus) is the natural reservoir of puumala virus (puuv), a species in the genus hantavirus. puuv is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. factors that influence hantavirus transmission within host populations are not well understood. we evaluated a number of factors influencing on the association of increased puuv infection in bank voles captured in a region in northern sweden endemic for the virus. l ... | 2002 | 12194768 |
| behavioral, physiologic, and habitat influences on the dynamics of puumala virus infection in bank voles (clethrionomys glareolus). | populations of bank voles (clethrionomys glareolus) were monitored during a 4-year study in southern belgium to assess the influence of agonistic behavior, reproductive status, mobility, and distribution of the rodents on the dynamics of puumala virus (abbreviation: puuv; genus: hantavirus) infection. concordance was high between data from serologic testing and results of viral rna detection. wounds resulting from biting or scratching were observed mainly in adult rodents. hantavirus infection i ... | 2002 | 12194769 |
| human leukocyte antigen-b8-dr3 is a more important risk factor for severe puumala hantavirus infection than the tumor necrosis factor-alpha(-308) g/a polymorphism. | the tumor necrosis factor (tnf)-alpha(-308) g/a polymorphism (tnf-2) is in linkage disequilibrium with the human leukocyte antigen (hla)-b8-dr3 haplotype. both factors have been associated with severe puumala hantavirus-induced nephropathia epidemica (ne). to examine which part of this extended haplotype might show the strongest association with the outcome of ne, the hla-b, hla-drb1, and tnf-alpha(-308) alleles in 116 hospital-treated patients with ne were analyzed. the findings pointing to cli ... | 2002 | 12198621 |
| golgi localization of hantaan virus glycoproteins requires coexpression of g1 and g2. | the membrane glycoproteins g1 and g2 of hantaan virus (htnv; family bunyaviridae) are encoded on the m rna genome segment as a precursor polypeptide that is cotranslationally cleaved by host proteases. g1 and g2 accumulate in the golgi complex in cells following either virus infection or transfection with m segment cdna. however, there are conflicting reports in the literature concerning the golgi targeting of separately expressed g1. to resolve these differences, a series of m segment and g1 co ... | 2002 | 12202203 |
| long-lived memory t lymphocyte responses after hantavirus infection. | puumala virus (puuv) is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs), which is an important public health problem in large parts of europe. we examined the memory cytolytic t lymphocyte (ctl) responses in 13 finnish individuals who had hfrs between 1984 and 1995. in seven of these donors, we detected virus-specific ctl responses against the puuv nucleocapsid (n) protein after in vitro stimulation with puuv. six novel cd8(+) ctl epitopes were defined on the n protein and ... | 2002 | 12208874 |
| pcr-generated linear dna fragments utilized as a hantavirus dna vaccine. | the field of dna vaccines has grown rapidly, and since most such vaccines involve the inoculation of large circular dna molecules previously propagated in bacteria, several inconveniences (e.g. the presence of antibiotic resistance genes, impurities from bacterial cultures or inefficient uptake of the large and bulky plasmid dna molecules to the nucleus) are debated. in this study, we have explored the possibility of using smaller and more flexible pcr-generated linear dna fragments instead. pho ... | 2002 | 12213408 |
| [results of study of a combined focus of leptospirosis and hantavirus infection in the primor'e territory]. | survey of the circulation and spread of causative agents of the infection among wild rodents may predict the activation of an epizootic process and the aggravation of an epidemic situation. the status of a combined focus of leptospirosis and hantavirus infection in the primorye territory was studied. it was found that mixed infection may run in pocket mice (phascogalinae) under natural conditions. | 2002 | 12214522 |
| serological evidence of hantavirus in humans and rodents in barbados. | 2002 | 12217714 | |
| encephalitis due to a hantavirus infection. | 2002 | 12217734 | |
| andes virus associated with hantavirus pulmonary syndrome in northern argentina and determination of the precise site of infection. | hantavirus pulmonary syndrome (hps) has been documented in the salta and jujuy provinces of northern argentina since 1991 and 1997, respectively, accounting for almost 50% of the cases of hps reported in this country. andes (and) virus, specifically the and virus nort lineage, was previously associated with human disease in this region. genetic analysis of viral medium rna segments obtained from 18 hps cases showed the existence of three and virus nort sublineages co-circulating in these two pro ... | 2002 | 12224579 |
| hantavirus infections in latvia. | in order to investigate the presence of hantavirus infections in latvia, 333 randomly selected human serum samples were screened using an enzyme-linked immunoassay. fifteen samples were positive for hantavirus-specific igg and were subsequently serotyped using a focus reduction neutralization test. fourteen of these samples neutralized at least one of the hantaviruses included in the test, indicating a 4.2% overall seroprevalence in latvia. among 14 focus reduction neutralization test-positive s ... | 2002 | 12226698 |
| [bunyavirus--virus and host relationship: the coevolution between hantavirus and rodent]. | 2002 | 12227181 | |
| spatiotemporal patterns in the hantavirus infection. | we present a model of the infection of hantavirus in deer mouse, peromyscus maniculatus, based on biological observations of the system in the north american southwest. the results of the analysis shed light on relevant observations of the biological system, such as the sporadical disappearance of the infection, and the existence of foci or "refugia" that perform as reservoirs of the virus when environmental conditions are less than optimal. | 2002 | 12241389 |
| detection of hantavirus infection in hemolyzed mouse blood using alkaline phosphatase conjugate. | conventional methods such as elisa and culture require a long time for the determination of viral infections. fast and reliable instrumentation suitable for operation under field conditions that allows rapid detection of hantavirus in rodent populations in the wild, would be a substantial improvement over these methods. in response to this challenge, a prototype amperometric immunosensor based on highly dispersed immunoelectrode for rapid, sensitive and quantitative assay of hantavirus in mice b ... | 2002 | 12243909 |
| yeast-expressed puumala hantavirus nucleocapsid protein induces protection in a bank vole model. | hantaviruses are rodent-borne agents that cause severe human diseases. the coding sequences for the authentic and a his-tagged puumala hantavirus (puuv) nucleocapsid (n) protein were expressed in yeast (saccharomyces cerevisiae). n-specific monoclonal antibodies demonstrated native antigenicity of the two proteins. all bank voles vaccinated with the his-tagged n protein in freund's adjuvant (n=12) were defined as completely protected against subsequent virus challenge, based on the absence of vi ... | 2002 | 12297397 |
| [seroprevalence of antibodies against hantavirus in 10 communities of the ix region of chile where hantavirus infection were diagnosed]. | thirty six cases of clinical hantavirus cardiopulmonary syndrome occurred in the ix region of chile. most of these patients were young males, farm or timber workers, who lived near the andes mountain chain. | 2002 | 12043366 |
| identification of tula hantavirus in pitymys subterraneus captured in the cacak region of serbia-yugoslavia. | atypical serum neutralizing antibody responses to prototype strains of puumala viruses in some patients with hemorrhagic fever with renal syndrome (hfrs) have long suggested the existence of other hantaviruses in the balkans. | 2002 | 12044299 |
| cross-protection against challenge with puumala virus after immunization with nucleocapsid proteins from different hantaviruses. | hantaviruses are rodent-borne agents that cause hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome in humans. the nucleocapsid protein (n) is relatively conserved among hantaviruses and highly immunogenic in both laboratory animals and humans, and it has been shown to induce efficient protective immunity in animal models. to investigate the ability of recombinant n (rn) from different hantaviruses to elicit cross-protection, we immunized bank voles with rn from puumala (puuv) ... | 2002 | 12050380 |
| hantavirus-specific antibodies in rodents and humans living in kuwait. | hantaviruses are found in widely scattered areas of the world and are transmitted by inhalation of virus-contaminated aerosols of rodent excreta. the present study was undertaken in kuwait to investigate the serological evidence for hantavirus infection in rodents and humans. sera were collected from 283 wild rodents and 183 human subjects (46 kuwaitis and 137 non-kuwaitis). the rodent sera were investigated for the presence of antibodies against the seoul and puumala strains of the hantaviruses ... | 2002 | 12052569 |
| acute renal failure caused by leptospirosis and hantavirus infection in an urban hospital. | background: leptospirosis (ls) and hantavirus (hv) infection have many common clinical manifestations, including acute renal failure. in as much as clinical experience with these diseases is quite limited in urban centers, we identified cases of leptospira and hv-induced acute renal failure and compared the clinical course and evolution of these diseases. methods: patients hospitalized with acute renal failure, suspected to be caused by infection, were retrospectively studied over a 13-year peri ... | 2002 | 12067823 |
| tula virus infection associated with fever and exanthema after a wild rodent bite. | reported here is the first case of human acute infection with tula virus, which occurred in a 12-year-old boy in switzerland. this hantavirus had been considered apathogenic to humans, and in switzerland only tulv-genome sequences have been demonstrated in wild rodents to date. in this case, paronychia, fever and exanthema occurred after the patient was bitten by a wild rodent, indicating an unusual route of hantavirus transmission. thus, tula virus infection should be taken into account in pati ... | 2002 | 12072943 |
| viral agents as biological weapons and agents of bioterrorism. | multiple viral agents have been classified by the cdc as potential weapons of mass destruction or agents for biologic terrorism. agents such as smallpox, viral hemorrhagic fever viruses, agents of viral encephalitis, and others are of concern because they are highly infectious and relatively easy to produce. although dispersion might be difficult, the risk is magnified by the fact that large populations are susceptible to these agents and only limited treatment and vaccination strategies exist. ... | 2002 | 12074486 |
| genetic vaccines protect against sin nombre hantavirus challenge in the deer mouse (peromyscus maniculatus). | we used a deer mouse (peromyscus maniculatus) infection model to test the protective efficacy of genetic vaccine candidates for sin nombre (sn) virus that were known to provoke immunological responses in balb/c mice (bharadwaj et al., vaccine 17, 2836-2843, 1999 ). protective epitopes were localized in each of four overlapping cdna fragments that encoded portions of the sn virus g1 glycoprotein antigen; the nucleocapsid gene also was protective. the protective efficacy of glycoprotein gene fragm ... | 2002 | 12075094 |
| [hantavirus in human and rodent population in an endemic area for hantavirus pulmonary syndrome in argentina]. | this paper analyzed the prevalence and distribution of serological reactivity to hantavirus (antibody against andes virus) of human population exposed to hantavirus and rodents trapped in the studied area. this study was developed in salta (orán and san martín departments), area with the highest incidence for hantavirus pulmonary syndrome (hps) in argentina. in december 1997, 453 healthy people were studied by serology and 39 rodents by serology and pcr. the studied individuals were distributed ... | 2002 | 11965841 |
| andes virus and first case report of bermejo virus causing fatal pulmonary syndrome. | two suspected hantavirus pulmonary syndrome (hps) cases from bolivia occurred in may and july 2000 and were confirmed by enzyme-linked immunosorbent assay (elisa)-andes using n-andes recombinant antigen serology. clot rnas from the two patients were subjected to reverse transcription-polymerase chain reaction (pcr) amplification and sequencing. we describe two characterized cases of hps. one was caused by infection with bermejo virus and the other with andes nort viral lineage, both previously o ... | 2002 | 11971782 |
| emerging infectious diseases in wildlife. | the processes which give rise to emerging infectious diseases of wildlife can be categorised as follows: ecosystem alterations of anthropogenic or natural origin; movement of pathogens or vectors, via human or natural agency; and changes in microbes or in the recognition of emerging pathogens due to advances in the techniques of epidemiology. these are simplistic divisions because factors influencing the emergence of diseases of wild animals generally fall into more than one category. mycoplasmo ... | 2002 | 11974625 |
| [changes in the epidemiology of infectious diseases in chile from 1990 to 2000]. | in the last decade in chile, there was a large reduction in the rate of communicable diseases, especially typhoid fever, and those preventable through the universal vaccination programs. exceptions were hepatitis a and pertussis. the reduction in tuberculosis, has lead the country to a threshold in which the elimination of tuberculosis as a public health problem is reachable. the hiv epidemic is still expanding, at higher rate among women and heterosexual men, keeping drug addiction as a low ran ... | 2002 | 12090099 |
| first human isolate of hantavirus (andes virus) in the americas. | we isolated andes virus (formal name: andes virus [andv], a species in the genus hantavirus), from serum of an asymptomatic 10-year-old chilean boy who died 6 days later of hantavirus pulmonary syndrome (hps). the serum was obtained 12 days after his grandmother died from hps and 2 days before he became febrile. no hantavirus immunoglobulin (ig) g or igm antibodies were detected in the serum sample. after three blind passages, andv antigens were detected in vero e6 cells by immunofluorescence as ... | 2002 | 12095430 |
| shedding and intracage transmission of sin nombre hantavirus in the deer mouse (peromyscus maniculatus) model. | the mechanism(s) by which sin nombre (sn) hantavirus is maintained in deer mouse populations is unclear. field studies indicate that transmission occurs primarily if not exclusively via a horizontal mechanism. using an experimental deer mouse infection model in an outdoor laboratory, we tested whether infected rodents shed sn virus in urine, feces, and saliva, whether infected mice transmit infection to naïve cage mates, and whether infected dams are able to vertically transmit virus or antibody ... | 2002 | 12097572 |
| wild-type puumala hantavirus infection induces cytokines, c-reactive protein, creatinine, and nitric oxide in cynomolgus macaques. | hantaviruses cause two severe human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). approximately 200,000 cases are reported annually, and there is to date no specific treatment available. a major obstacle in studying the medical aspects of hfrs and hps has been the lack of an adequate animal model. here we show that infection of cynomolgus macaques by wild-type puumala hantavirus resulted in typical signs of hfrs including lethargy, anorexia, prot ... | 2002 | 11739712 |
| first reported case of imported hantavirus pulmonary syndrome in europe. | 2002 | 11749764 | |
| radiographic findings in 20 patients with hantavirus pulmonary syndrome correlated with clinical outcome. | hantavirus is a rare rodent-borne pathogen responsible for the hantavirus pulmonary syndrome. the objective of this study was to review the clinical and radiographic findings of patients presenting with hantavirus pulmonary syndrome in northern alberta, canada. | 2002 | 11756112 |
| the rna binding domain of the hantaan virus n protein maps to a central, conserved region. | the nucleocapsid (n) protein of hantaviruses encapsidates both viral genomic and antigenomic rnas, although only the genomic viral rna (vrna) is packaged into virions. to define the domain within the hantaan virus (htnv) n protein that mediates these interactions, 14 n- and c-terminal deletion constructs were cloned into a bacterial expression vector, expressed, and purified to homogeneity. each protein was examined for its ability to bind the htnv s segment vrna with filter binding and gel elec ... | 2002 | 11884555 |
| transfection-mediated generation of functionally competent tula hantavirus with recombinant s rna segment. | since the discovery of rna recombination in polioviruses, there has been a general belief that this mechanism operates only in positive-sense rna viruses. recently, studying wild-type tula hantavirus, we observed a mosaic-like structure of the s rna segment that was consistent with generation by recombination between viruses from two genetic lineages. here we show transfection-mediated rescue of tula virus carrying recombinant s rna segment. independent attempts yielded s rna molecules of simila ... | 2002 | 11889055 |
| [puumala virus infection (nephropathia epidemica) as different diagnosis of acute renal failure]. | puumala virus infection (nephropathia epidemica) as different diagnosis of acute renal failure. | 2002 | 11894176 |
| serological divergence of dobrava and saaremaa hantaviruses: evidence for two distinct serotypes. | in order to investigate the serological relationship of dobrava hantavirus (dobv, originating from slovenia) and the dobrava-like saaremaa virus (saav, recently discovered in estonia) we analysed 37 human serum samples, 24 from estonia and 13 from the balkans, by focus reduction neutralization test (frnt). most of the estonian sera (19), including all sera from saaremaa island (12), reacted with higher frnt end-point titres to the local saav; the majority of them (15 and 11, respectively), with ... | 2002 | 11895097 |
| analysis of hantavirus genetic diversity in argentina: s segment-derived phylogeny. | nucleotide sequences were determined for the complete s genome segments of the six distinct hantavirus genotypes from argentina and for two cell culture-isolated andes virus strains from chile. phylogenetic analysis indicates that, although divergent from each other, all argentinian hantavirus genotypes group together and form a novel phylogenetic clade with the andes virus. the previously characterized south american hantaviruses laguna negra virus and rio mamore virus make up another clade tha ... | 2002 | 11907216 |
| hantavirus nucleocapsid protein interacts with the fas-mediated apoptosis enhancer daxx. | hantaviruses cause two severe diseases, haemorrhagic fever with renal syndrome in eurasia and hantavirus pulmonary syndrome in the americas. to understand more about the molecular mechanisms that lead to these diseases, the associations of puumala virus nucleocapsid protein (puuv-n) with cellular proteins were studied by yeast two-hybrid screening. daxx, known as an apoptosis enhancer, was identified from a hela cdna library and its interaction with puuv-n was confirmed by gst pull-down assay, c ... | 2002 | 11907324 |
| identification of a cell surface 30 kda protein as a candidate receptor for hantaan virus. | cellular receptors play an important role in virus pathogenesis. as a first step in virus infection, viruses attach to specific receptors on the surface of cells; hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. to date, the identity of the hantaan virus host cell receptor remains unknown. to determine the protein on the cell surface to which hantaan virus binds, a virus overlay protein-binding assay was performed with radiolabelled virus. a 30 kda ... | 2002 | 11907325 |
| the assessment of human health risks from rodent-borne diseases by means of ecological studies of rodent reservoirs. | zoonoses in general, and more specifically rodent-borne diseases, have proven to be of increasing importance in recent decades. the study of vector biology, therefore, is the foundation for understanding the infection mechanisms for humans. military operations on the european and asian continent have been substantially affected by hantavirus infections during world war i and world war ii, the korean war, and the more recent events in bosnia. the recently discovered hantavirus serotypes with high ... | 2002 | 11799818 |
| increased glomerular permeability in patients with nephropathia epidemica caused by puumala hantavirus. | nephropathia epidemica (ne) is the scandinavian type of haemorrhagic fever with renal syndrome. usually heavy transient proteinuria is present at the onset of the disease. | 2002 | 11812874 |
| discriminators between hantavirus-infected and -uninfected persons enrolled in a trial of intravenous ribavirin for presumptive hantavirus pulmonary syndrome. | to provide a potentially therapeutic intervention and to collect clinical and laboratory data during an outbreak of hantavirus pulmonary syndrome (hps), 140 patients from the united states with suspected hps were enrolled for investigational intravenous ribavirin treatment. hps was subsequently laboratory confirmed in 30 persons and not confirmed in 105 persons with adequate specimens. patients with hps were significantly more likely than were hantavirus-negative patients to report myalgias from ... | 2002 | 11774075 |
| [detection of the expression of alpha3-integrin on hantavirus permissive cells]. | to express and purify human alpha3-integrin to serve as the antigen to prepare its antibody and to separate the vero cell clones without expression of alpha3-integrin. | 2002 | 11986744 |
| [molecular cloning,nucleotides sequence and transient expression of s and m genome segment of hantavirus strain 84fli]. | to sequence, analyze and express the nucleotide sequences of s and m segments of hantavirus strain 84fli. | 2002 | 11986746 |
| [serologic detection of hantavirus antibodies]. | within the framework of diagnosis of highly dangerous infections in the army of the czech republic serological assessment of hantaviral antibodies was introduced. because military units in the field can be in contact with sources of hantaviral infections, the authors investigated this population sample. in 1999-2000 a total of 542 healthy subjects were examined (age 20-40 years). hantaviral antibodies were detected in 9. in three antibodies against the serotype hantaan were found, in two antibod ... | 2002 | 11987584 |
| sequence and expression analysis of deer mouse interferon-gamma, interleukin-10, tumor necrosis factor, and lymphotoxin-alpha. | deer mice (peromyscus maniculatus) are the principal host species of sin nombre (sn) virus, the primary etiologic agent of hantavirus cardiopulmonary syndrome in north america. the disease is a cytokine-mediated immunopathology characterized by pulmonary mononuclear infiltrates without discernible viral pathology. infected deer mice remain life-long carriers and virus is found in many organs, including the lungs, but without pathology. it is unclear how deer mice respond to sn virus because no t ... | 2002 | 11991673 |
| [a new case of hantavirus disease in spain?]. | 2002 | 11996751 | |
| tula hantavirus in belgium. | european common voles (microtus arvalis), captured in belgium in 1999, were proven by molecular as well as by serological techniques to be infected with tula hantavirus (tulv). this is the first evidence for the presence of tulv in this country. no indication of spill-over infections of puumala virus, known to be highly endemic among bank voles (clethrionomys glareolus) within the same geographical regions as the trapped tulv-infected common voles, was observed. together with previous reports on ... | 2002 | 12002543 |
| hantavirus nucleocapsid protein coiled-coil domains. | the nucleocapsid (n) proteins of hantaviruses such as the sin nombre virus (snv) bind to membranes and viral rnas, associate with transcription and replication complexes, and oligomerize during the process of virus assembly. n proteins trimerize in vitro and in vivo, and associate via assembly domains at their amino- and carboxyl-terminal ends. because structure prediction algorithms suggested that n protein residues 3-75 form two coiled-coil motifs separated by an intervening kink or turn seque ... | 2002 | 12019266 |
| hantaviruses: an overview. | hantaviruses are a newly emerging group of rodent-borne viruses that have significant zoonotic potential. human infection by hantaviruses can result in profound morbidity and mortality, with death rates as high as 50%, and potentially long-term cardiovascular consequences. hantaviruses are carried by peridomestic and wild rodents worldwide and have occasionally been linked to infections in laboratory rodents. because these viruses have been associated with significant human disease, they have be ... | 2002 | 12022401 |
| hantavirus infection with marked sinus bradycardia, taiwan. | 2002 | 12023927 | |
| double trouble: hemorrhagic fever with renal syndrome and leptospirosis. | the clinical picture of hemorrhagic fever with renal syndrome can closely mimic that of unicteric leptospirosis and vice versa. this is the first description of dual infection with dobrava virus and leptospira and alteration of immune parameters in a croatian soldier. | 2002 | 12030403 |
| assessment of ecologic and biologic factors leading to hantavirus pulmonary syndrome, colorado, u.s.a. | to understand the ecologic parameters of sin nombre virus (snv; family bunyaviridae, genus hantavirus) infections in the deer mouse (peromyscus maniculatus), environmental variables impacting the rodent populations, and the conditions under which snv is amplified. this may help us understand the antecedents of human risk for developing hantavirus pulmonary syndrome (hps) as a consequence of snv infection. | 2002 | 12035141 |
| hunting down hantavirus. | 2002 | 12035655 | |
| genetics of hantaviruses: implications to taxonomy. | hantaviruses (genus hantavirus, family bunyaviridae) represent a prime example of emerging viruses. since isolation of the prototype hantaan virus in the late 70s more than 20 new species have been described and the number is increasing fast thus demanding for a more refined classification. taking into account that hantaviruses are difficult to isolate in cell culture, one should not be surprised that most of the "newcomers" were first described as distinct hantavirus genotypes. moreover, the on ... | 2002 | 12038679 |
| acute disseminated encephalomyelitis following nephropathia epidemica. | acute disseminated encephalomyelitis (adem) is an acute monophasic inflammatory and demyelinating disease of the central nervous system (cns) occurring days to weeks after a virus infection or vaccination. nephropathia epidemica (ne) is a haemorrhagic fever with renal syndrome caused by puumala virus, with endemic regions in europe, especially scandinavia and western russia. we describe a case of severe nephropathia epidemica requiring dialysis, followed by severe cns symptoms caused by adem. to ... | 2002 | 11939950 |
| hantavirus pulmonary syndrome: the new american hemorrhagic fever. | the recognition of hantavirus pulmonary syndrome (hps) after the investigation of a cluster of unexplained respiratory deaths in the southwestern united states during the spring of 1993 showcased our ability to recognize new and emerging diseases, given the correct juxtaposition of a new clinical entity with circumscribed epidemiologic features that are analyzed with novel diagnostic methods. in less than a decade, hps has become established as a pan-american zoonosis due to numerous viruses mai ... | 2002 | 11941549 |
| herpesvirus hijacks an integrin. | 2002 | 11944033 | |
| mapping of b-cell epitopes in the nucleocapsid protein of puumala hantavirus. | hantavirus nucleocapsid protein (n) has been proven to induce highly protective immune responses in animal models. the knowledge on the mechanisms behind n-induced protection is still limited, although recent data suggest that both cellular and humoral immune responses are of importance. for a detailed b-cell epitope mapping of puumala hantavirus (puuv) n, we used recombinant n derivatives of the russian strain cg18-20 and the swedish strain vranica/hällnäs, as well as overlapping synthetic pept ... | 2002 | 11952140 |
| characteristics of puumala and dobrava infections in croatia. | in this study, two different hantaviruses, puumala virus (puuv) and dobrava virus (dobv), were demonstrated for the first time to coexist and cause hemorrhagic fever with renal syndrome (hfrs) in croatia. phylogenetic analysis showed some differences among the nucleotide sequences of puuv originating from dinara mountain, which was more closely related to austrian puuv than other croatian puuv from mala kapela mountain. more consistency was found among the croatian dobv. hfrs was verified in 85 ... | 2002 | 11857535 |
| the multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes. | multimerization of the hantaan virus nucleocapsid protein (np) in hantaan virus-infected vero e6 cells was observed in a competitive enzyme-linked immunosorbent assay (elisa). recombinant and truncated nps of hantaan, seoul, and dobrava viruses lacking the n-terminal 49 amino acids were also detected as multimers. although truncated nps of hantaan virus lacking the n-terminal 154 amino acids existed as a monomer, those of seoul and dobrava formed multimers. the multimerized truncated np antigens ... | 2003 | 12502810 |
| persistent sin nombre virus infection in the deer mouse (peromyscus maniculatus) model: sites of replication and strand-specific expression. | to address sin nombre (sn) virus persistence in deer mice, we sacrificed experimentally infected deer mice at eight time points from day 21 to day 217 postinoculation (p.i.) and examined their tissues for viral nucleocapsid (n) antigen expression and both negative-strand (genomic) and positive-strand (replicative/mrna) viral s segment rna titers. all the animals that we inoculated developed persistent infections, and sn virus could be isolated from tissues throughout the course of infection. the ... | 2003 | 12502867 |
| hantavirus pulmonary syndrome-associated hantaviruses contain conserved and functional itam signaling elements. | hantaviruses infect human endothelial and immune cells, causing two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we have identified key signaling elements termed immunoreceptor tyrosine-based activation motifs (itams) within the g1 cytoplasmic tail of all hps-causing hantaviruses. itams direct receptor signaling within immune and endothelial cells and the presence of itams in all hps-causing hantaviruses provides a means for altering norma ... | 2003 | 12502882 |
| chilean research throws light on hantavirus transmission. | 2003 | 12505021 | |
| ribavirin causes error catastrophe during hantaan virus replication. | except for ribavirin, no other antiviral drugs for treating hantaviral diseases have been identified. it is well established that ribavirin will inhibit the production of infectious hantaan virus (htnv); however, its mechanism of action is unknown. to characterize the inhibitory effect of ribavirin on htnv, the levels of viral rnas, proteins, and infectious particles were measured for 3 days posttreatment of htnv-infected vero e6 cells. htnv-infected cells treated with ribavirin showed a slight ... | 2003 | 12477853 |
| genetic interaction between distinct dobrava hantavirus subtypes in apodemus agrarius and a. flavicollis in nature. | dobrava virus (dobv) occurs in two different rodent species, apodemus flavicollis (dobv-af) and a. agrarius (dobv-aa). we sequenced the s and m genomic segments from sympatric dobv-af and dobv-aa strains which fell into two distinct genetic lineages. molecular phylogenetic analyses gave evidence for genetic reassortment between s and m segments of dobv-af and dobv-aa and indicated homologous recombination events in dobv evolution. dobv-af and dobv-aa are distinct but also subject to genetic exch ... | 2003 | 12477889 |
| generation of an hfrs patient-derived neutralizing recombinant antibody to hantaan virus g1 protein and definition of the neutralizing domain. | hantaan virus (htnv) in the hantavirus genus, family bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (hfrs). we prepared a combinatorial phage display library of human fabs to htnv from rna extracted from the blood lymphocytes of a convalescent hfrs patient. we selected two g1 glycoprotein-specific clones and one nucleocapsid protein (n)-specific clone from the fab library for further studies. the human fab antibodies were converted to igg form in baculovirus/ins ... | 2003 | 12436484 |
| hantaviruses. a short review. | hantaviruses are rodent viruses that have been identified as etiologic agents of 2 diseases of humans: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). this article presents a concise review of hantavirus biology, the medical features of hfrs and hps, and tests for the detection of hantavirus infections in humans. | 2003 | 12521363 |
| use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test. | a vesicular stomatitis virus (vsv) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. the recombinant vsv, in which the enveloped protein gene (g) was replaced by the green fluorescent protein gene and complemented with g protein expressed in trans (vsvdeltag*g), was kindly provided by m. a. whitt. 293t cells were transfected with plasmids for the expression of envelope glycoproteins (g1 and g2) of htnv or se ... | 2003 | 12522053 |
| seroprevalence of three emerging arboviral infections in kuwaiti nationals. | diseases caused by dengue, sandfly fever and hanta viruses pose a major health risk in many countries. we determined the threat of these arboviral infections through a serologic using enzyme linked immunosorbent assay (elisa) based tests. hantavirus-specific antibodies were also detected using immunofluorescence. of 499 samples tested for dengue virus igg antibodies l4% were as positive for dengue positive by all the elisa tests. among the 42 showing strong igg reactivity, only 1 was positive fo ... | 2003 | 15751918 |
| [using recombinant antigens of hantavirus to study the kinetics of serum iga, igg, igm antibodies in the acute-phase of hemorrhagic fever renal syndrome]. | to observe the features of serum specific iga, igg, igm antibodies in the acute phase of hemorrhagic fever renal syndrome (hfrs). | 2003 | 15340571 |
| serological survey of hantavirus in jardinopolis county, brazil. | hantaviruses are the causative agents of severe human diseases such as cardiopulmonary syndrome (cps), described for the first time in 1993. approximately 200 cases of this emergent disease were reported in brazil until august 2002, with a 40% fatality rate. in may 2001, a hantavirus serologic survey was carried out among 15-70-year-old inhabitants of jardinopolis county, state of são paulo, brazil. blood samples of 818 inhabitants from urban and rural areas were collected by digitopuncture and ... | 2003 | 12966548 |
| hemorrhagic fever with renal syndrome presenting with intrauterine fetal death. a case report. | hantavirus infection in pregnancy is rare. only 2 cases of hantavirus pulmonary syndrome have been reported in the english-language literature. we report a case of hemorrhagic fever with renal syndrome (hfrs) complicating pregnancy to alert clinicians to this rare possibility. | 2003 | 12971153 |
| synthesis of seoul virus rna and structural proteins in cultured cells. | seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). the virion has a tripartite (s, m, and l) negative-stranded rna genome, which is characteristic of the family bunyaviridae. however, the molecular basis of virus replication is not well known. we established a northern blot hybridization (nb) procedure using digoxygenin-labeled rna probes, to quantitate the hantaviral plus- and minus-strand rnas separately. virus rna replication was analyzed in infected vero e6 ... | 2003 | 14505081 |
| tyrosine residues direct the ubiquitination and degradation of the ny-1 hantavirus g1 cytoplasmic tail. | the hantavirus g1 protein contains a long c-terminal cytoplasmic tail of 142 residues. hantavirus pulmonary syndrome-associated hantaviruses contain conserved tyrosine residues near the c terminus of g1 which form an immunoreceptor tyrosine activation motif (itam) and interact with src and syk family kinases. during studies of the g1 itam we observed that fusion proteins containing the g1 cytoplasmic tail were poorly expressed. expression of g1 cytoplasmic tail constructs were dramatically enhan ... | 2003 | 14512526 |
| mapping of the regions involved in homotypic interactions of tula hantavirus n protein. | hantavirus nucleocapsid (n) protein has been suggested to form homodimers and homotrimers that are further integrated into the nucleocapsid filaments around the viral rna. here we report detailed mapping of the regions involved in the homotypic n protein interactions in tula hantavirus (tulv). peptide scan screening was used to define the interaction regions, and the mammalian two-hybrid assay was used for the functional analysis of n protein mutants. to study linear regions responsible for n pr ... | 2003 | 14512541 |
| complete sequence of the genome of the human isolate of andes virus chi-7913: comparative sequence and protein structure analysis. | we report here the complete genomic sequence of the chilean human isolate of andes virus chi-7913. the s, m, and l genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall gc content of 38.7%. these genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a rna-dependent rna polymerase of 2,152 amino acids. in addition, the genome also has other orfs coding for putative proteins of 34 ... | 2003 | 14513715 |
| prevalence of antibodies against hantaviruses among the adult population of the czech republic. | at least three hantavirus genospecies are circulating among wild rodents in europe: puumala, dobrava and tula, the two first being of clinical significance for humans as possible causative agents of haemorrhagic fever with renal syndrome. as many as 710 adult individuals randomly selected from 12 regions of the czech republic were screened for the presence of antibodies against hantaviruses. commercial sets with hantaan and puumala antigens were used for this purpose. five subjects showed igg re ... | 2003 | 14514172 |
| hantavirus infection in humans and rodents, northwestern argentina. | we initiated a study to elucidate the ecology and epidemiology of hantavirus infections in northern argentina. the northwestern hantavirus pulmonary syndrome (hps)-endemic area of argentina comprises salta and jujuy provinces. between 1997 and 2000, 30 hps cases were diagnosed in jujuy province (population 512,329). most patients had a mild clinical course, and the death rate (13.3%) was low. we performed a serologic and epidemiologic survey in residents of the area, in conjunction with a serolo ... | 2003 | 14519242 |