Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| reactivation of latent herpes simplex virus by adenovirus recombinants encoding mutant ie-0 gene products. | we have previously shown that adenovirus recombinants expressing functional icp0 reactivate latent herpes simplex virus type 2 (hsv-2) in an in vitro latency system. this study demonstrated that icp0, independent of other hsv gene products, is sufficient to reactivate latent hsv-2 in this in vitro system. to assess the effects of defined mutations in the sequence encoding icp0 (ie-0) on reactivation, seven in-frame insertion and three in-frame deletion mutants were moved into an adenovirus expre ... | 1990 | 2166826 |
| altered sensitivity to antiviral drugs of herpes simplex virus isolates from a patient with the acquired immunodeficiency syndrome. | acyclovir (acv)-resistant herpes simplex virus type 2 (hsv-2) was isolated from a patient with acquired immunodeficiency syndrome after long-term but intermittent acv therapy. these thymidine kinase-defective isolates were sensitive in vitro to foscarnet. while combined therapy with acv and interferon produced only partial clinical improvement, the in vitro effect of this combination against an acv-resistant isolate from the patient was strongly synergistic. a short course (10-12 days) of intrav ... | 1990 | 2167340 |
| glycoprotein c-dependent attachment of herpes simplex virus to susceptible cells leading to productive infection. | herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein c (gc) and one independent of that glycoprotein. thus, whereas the polycations neomycin and polylysine inhibit attachment but not entry of already attached herpes simplex virus 1 (hsv-1) into baby hamster kidney (bhk) cell line, they have no effe ... | 1990 | 2167550 |
| high level expression in 293 cells of the herpes simplex virus type 2 ribonucleotide reductase subunit 2 using an adenovirus vector. | the herpes simplex viruses (hsv-1 and hsv-2) encode a ribonucleotide reductase consisting of two non-identical subunits (rr1 and rr2) which associate to form the active holoenzyme. to facilitate the purification and subsequent biochemical characterization of this enzyme, we have cloned the small subunit 2 of the hsv-2 ribonucleotide reductase (rr2hsv-2) in a helper-independent adenovirus type 5 vector under the control of the adenovirus type 2 major late promoter. after infection of 293 cells wi ... | 1990 | 2167932 |
| papillomavirus trans-activator protein e2 activates expression from the promoter for the ribonucleotide reductase large subunit from herpes simplex virus type 2. | activation of the herpes simplex virus type 2 (hsv-2) large subunit of the ribonucleotide reductase (icp10) gene by papillomavirus dna encoding the e2 or e7 proteins was studied directly by immunofluorescence or by chloramphenicol acetyltransferase (cat) analysis with hybrid icp10 or ie175 and 38k promoter constructions. cotransfection with bovine papillomavirus type 1 or human papillomavirus type 16 (hpv-16) e2 dna enhanced cat expression from constructions in which cat is regulated by the icp1 ... | 1990 | 2167936 |
| rapid detection of herpes simplex virus in clinical samples by flow cytometry after amplification in tissue culture. | murine monoclonal antibodies (mabs) against herpes simplex virus type 1 and 2 (hsv-1 and -2, respectively) nuclear antigens were used to identify cells infected with hsv-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of mrc-5 cell monolayers. the results indicate that mabs q1, q2, and h-640 detect hsv-1- and hsv-2-infected cells, mab sd-1 detects hsv-2- but not hsv-1-infected cells, and mab 58-s detects hsv-1- but not hsv-2-infected cells. mab ... | 1990 | 2168440 |
| [epidemiologic analysis of dna's of herpes simplex virus, type 1 and type 2 isolates obtained from family members and from different lesions of individuals]. | we analyzed herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infections in 6 families, to see whether or not a single strain had infected members of each family, analyzing dna's of viral isolates with restriction endonucleases. we also analyzed 29 isolates from 13 patients to examine if herpetic lesions on different sites of each patient's body had been caused by a single strain. in 4 cases of family infection and 10 cases of individual infection with multiple site manifestations, electroph ... | 1990 | 2169506 |
| escherichia coli-derived envelope protein gd but not gc antigens of herpes simplex virus protect mice against a lethal challenge with hsv-1 and hsv-2. | immunization studies with hsv-1 and hsv-2 envelope proteins expressed in escherichia coli were performed. after active immunization of mice with a gd-1 antigen (leu53-ala312) expressed as a fusion protein, the animals were protected from a lethal challenge with hsv-1 and hsv-2. in addition, antisera from rabbits immunized with the same gd-1 antigen also conferred passive immunity to mice against a challenge infection with either hsv-1 or hsv-2. in contrast to these successful gd-1 protection exp ... | 1990 | 2169577 |
| infection of vascular endothelial cells with herpes simplex virus enhances tissue factor activity and reduces thrombomodulin expression. | latent infection of vascular cells with herpes-viruses may play a pathogenic role in the development of human atherosclerosis. in a previous study, we found that cultured human umbilical vein endothelial cells (huvecs) infected with herpes simplex virus 1 (hsv-1) became procoagulant, exemplified both by their enhanced assembly of the prothrombinase complex and by their inability to reduce adhesion of platelets. we now report two further procoagulant consequences of endothelial hsv infection: los ... | 1990 | 2169619 |
| [detection of herpes simplex virus, varicella zoster virus and cytomegalovirus in aphthous stomatitis]. | attempts were made to demonstrate herpes simplex virus (hsv) type 1 and type 2, varicella zoster virus (vzv) and cytomegalovirus (cmv) in specimens obtained from aphthous ulceration lesions by the immunofluorescent method using fluorescein-labeled monoclonal antibodies. hsv-1 and vzv were detected in 2 and 4 out of 30 patients, respectively. although almost all viruses that can infect the oral cavity could occasionally cause stomatitis, neither hsv-2 nor cmv was not found in this study. vzv was ... | 1990 | 2170607 |
| characterization of herpes simplex virus type 2 transcription during latent infection of mouse trigeminal ganglia. | using a cornea trigeminal ganglion model, we have investigated transcription by herpes simplex virus type 2 (hsv-2) during latency in mice. latency was verified 2 months postinoculation by reactivation of hsv-2 after explant cocultivation of trigeminal ganglia from the majority of mice (83%). transcription during latent hsv-2 infection was limited to the repeat regions of the viral genome as determined by in situ hybridization using restriction fragment probes representing 100% of the hsv-2 geno ... | 1990 | 2170675 |
| [guidelines for management of genital herpes infection in the experience of a health care center for sexually transmitted diseases in buenos aires (argentina)]. | during two years all clinical genital lesions compatible with herpes simplex virus (hsv) etiology were studied systematically through intent of virus isolation in bhk cells. isolation of virus was confirmed in 85% of the 206 cases studied. all seems to indicate that around the 20% of the isolations belong to the type hsv1 and the rest to the type hsv2. viral antigen demonstration, by direct and/or indirect technics with antibodies poly or monoclonals shows to be less sensitive than the virus iso ... | 1990 | 2170786 |
| myristylation and polylysine-mediated activation of the protein kinase domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the amino-terminal domain of the large subunit of herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (icp10) was previously shown to possess protein kinase (pk) activity that localizes to the cytosolic, cytoskeletal, and plasma membrane fractions. further studies of the pk domain using computer-assisted sequence analysis have identified a single transmembrane segment and fatty acid incorporation findings indicate that icp10 is myristylated. myristylation does not depend on a viral enzy ... | 1990 | 2171204 |
| the nucleotide sequence of the equine herpesvirus 4 gc gene homologue. | the genomic position of an equine herpesvirus 4 (ehv-4) gene homologue of the herpes simplex virus 1 (hsv-1) gc gene was determined by southern analysis and dna sequencing. the gene lies within a 2-kbp bg/ii-ecori fragment mapping between 0.15 and 0.17 within the long unique component of the ehv-4 genome and is transcribed from right to left. putative promoter elements were identified upstream of the 1455-bp open reading frame which encodes a 485-amino-acid protein of unglycosylated molecular we ... | 1990 | 2171212 |
| herpes simplex virus genes controlling reactivation from latency in rabbit eye model. | using the rabbit eye model of latency, herpes simplex virus type 1 strain mckrae invariably reactivated after epinephrine iontophoresis, whereas type 2(hsv-2) virus strain hg 52 failed to reactivate. both strains established a latent infection with the same frequency. to identify the viral genes involved in this reactivation difference, intertypic recombinants were selected following cotransfection of intact mckrae dna and xba i or hpa i cleaved hg52 dna. eleven separately obtained recombinants ... | 1990 | 2172160 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| [the possibility of viral etiology in cervical carcinogenesis]. | oncogenic potential of herpes simplex virus type 2 (hsv-2) and human papillomavirus (hpv) which are both associated with occurrence of cervical cancer and the mechanism of oncogenesis by these viruses were investigated by transformation experiments in vitro. the results were obtained as follows. 1) hsv-2 induced neoplastic transformation of normal diploid cells is a multistep process. cervical cancer associated antigen ag-4 is encoded within the specific region of hsv-2 dna which converts immort ... | 1990 | 2172417 |
| evolutionary relationships of virion glycoprotein genes in the s regions of alphaherpesvirus genomes. | the short region in the genome of herpes simplex virus type 1 contains a contiguous array of five genes (us4, us5, us6, us7 and us8) which encode known or proposed virion-surface glycoprotein species. counterparts for certain of these have been described in the genomes of other alphaherpesviruses, namely herpes simplex virus type 2, pseudorabies virus and varicella-zoster virus. within each of the us4-, us6- and us7-related sets, the amino acid sequences are most conserved in a region containing ... | 1990 | 2172448 |
| herpes simplex virus type 2 meningitis without genital lesions: an immunoblot study. | two sexually active female patients presented with acute meningitis. the csf abnormalities were severe and persistent. in spite of the absence of genital lesions, serological studies revealed a primary infection by herpes simplex virus type 2. an immunoblot study revealed intrathecal synthesis of anti-herpes antibodies early in the course of the disease. | 1990 | 2172470 |
| viral etiology of chronic cervicitis and its therapeutic response to a recombinant interferon. | chronic cervicitis was shown to be related to papillomavirus type 16(hpv-16), herpes simplex virus type 2 and cytomegalovirus (cmv) infections as demonstrated by dna hybridization technique and virus isolation method from samples taken from erosive and normal cervices. after one course of treatment with recombinant interferon alpha 1 (rifn-alpha 1), 93.8% of cases showed clinical improvement and 60% marked improvement. the hpv-16 and hsv detection rates dropped down significantly after rifn-alph ... | 1990 | 2173655 |
| mechanisms of inhibition of herpes simplex virus type 2 growth by 28-mer phosphorothioate oligodeoxycytidine. | the 28-mer phosphorothioate oligodeoxycytidine (s-(dc)28) has been reported previously to be a strong inhibitor of herpes simplex virus type 2 (hsv-2) dna polymerase and hsv-2 growth in cell culture. in this study, the mechanism of action of s-(dc)28 was studied. s-(dc)28 was found to interfere with the adsorption of hsv-1 and hsv-2 to hela cells. hsv-2 infection, but not hsv-1, was found to potentiate the uptake of s-(dc)28 into hela cells. the enhanced uptake reached a plateau at 6-9 h postinf ... | 1990 | 2173699 |
| adenovirus infection in patients with kawasaki disease. | two outbreaks of kawasaki disease at different times and areas (kyoto in 1982 and sapporo in 1985) of japan were studied retrospectively for the presence of antibodies to adenoviruses and herpesviruses. only 2 of 12 (16.7%) consecutive acute phase sera of patients from the outbreak in 1982 and 1 of 10 (10.0%) sera from 1985 showed positive antibodies for the common adenovirus antigen by a complement fixation (cf) test, whereas 10 of 16 (62.5%) age- and sex-matched controls during the outbreak of ... | 1990 | 2173738 |
| effect of hexamethylene bisacetamide and cyclosporin a on recovery of herpes simplex virus type 2 from the in vitro model of latency in a human neuroblastoma cell line. | the goal of the present work was to examine whether hexamethylene bisacetamide (hmba) and cyclosporin a affect the recovery of herpes simplex virus type 2 (hsv-2) from an in vitro model of hsv-2 latency in human neuroblastoma cell line imr-32. imr-32 cells were infected with hsv-2 at a multiplicity of infection of 0.1 plaque-forming units/cell and were cultured at 40 degrees c for 14 days, resulting in the establishment of a model of hsv-2 latency in imr-32 cells. when the cultivation temperatur ... | 1990 | 2174735 |
| arabinofuranosylpyrrolo[2,3-d]pyrimidines as potential agents for human cytomegalovirus infections. | protection of the 3'- and 5'-hydroxyl groups of the nucleoside antibiotic toyocamycin (1) with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane was followed by (trifluoromethyl)sulfonylation of the 2'-hydroxyl group. a displacement of the resulting triflate ester moiety with lithium chloride, lithium bromide, sodium iodide, and lithium azide in hexamethylphosphoramide was followed by a removal of the disilyl moiety with tetra-n-butylammonium fluoride to afford the appropriate (2'-deoxy-2'-substitut ... | 1990 | 2175356 |
| neurovirulence of herpes simplex virus types 1 and 2 isolates in diseases of the central nervous system. | herpes simplex virus (hsv) isolates derived from the central nervous system of ten patients with hsv-1-induced encephalitis, one patient with multiple sclerosis, and 14 patients with hsv-2-induced meningitis were investigated for neurovirulence by assaying the ld50 after nose and intracerebral (i.c.) inoculation of mice. hsv-1 encephalitis strains were significantly more virulent after nose inoculation (i.e. neuroinvasive) when compared with hsv-1 isolates from patients with oral lesions only, w ... | 1990 | 2175706 |
| sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. | a newly developed herpes simplex virus (hsv) type 1 and type 2 enzyme immunoassay has been applied to over 40,000 sera from different populations in the us and 17 other countries on all 5 continents. the hsv-1 antibody patterns found permit the current and past socioeconomic status, as well as the extent of oral-genital sexual contact, to be deduced for various populations. the hsv-2 antibody results provide an objective measure of various aspects of sexual behavior: extent of polypartnerism in ... | 1990 | 2175939 |
| use of the pbs2 uracil-dna glycosylase inhibitor to differentiate the uracil-dna glycosylase activities encoded by herpes simplex virus types 1 and 2. | the bacteriophage pbs2 encoded uracil-dna glycosylase (ung) inhibitor was examined for its effect upon the nuclear ung activities of kb, hela, and vero cells infected with herpes simplex virus (hsv) type 1 or 2 and mock-infected cells. ung activity from hsv-1 infected cells exhibited the greatest sensitivity to inhibition by the inhibitor, while ung activity from cells infected with hsv-2 exhibited the greatest resistance. this differential effect was dependent upon the virus, cell line, and buf ... | 1990 | 2176221 |
| cytopathogenicity, drug susceptibility, and thymidine kinase activity of a retinovirulent herpes simplex virus type 2. | we investigated some of the biological and biochemical characteristics of a neuroinvasive, retinovirulent herpes simplex virus type 2 strain sl (hsv-2[sl]) and compared them with those of a neurovirulent, nonretinovirulent hsv-2 (186). hsv-2(sl) was shown to spread rapidly and produce large syncytium in vitro. hsv-2(sl) and hsv-2(186) were equally susceptible to acyclovir (acv) and thymine arabinoside (ara-t). however, hsv-2(sl) was fourfold and 44-fold more susceptible than hsv-2(186) to iodode ... | 1990 | 2176674 |
| inactivation of the shutoff gene (ul41) of herpes simplex virus types 1 and 2. | gene ul41 of herpes simplex virus type 1 (hsv-1) and the corresponding gene of hsv-2, which control the virion-mediated early suppression of cellular protein synthesis, have been inactivated by inserting a beta-galactosidase expression cassette into their coding regions. the resulting recombinants grew well in tissue culture, although with the type 2 recombinant viral protein synthesis was slightly delayed. as a result of inactivation of ul41 host protein synthesis was not suppressed in the pres ... | 1990 | 2177088 |
| antiviral activity and pharmacokinetics of hoe 602, an acyclic nucleoside, in animal models. | the acyclic nucleoside derivative hoe 602 (2-amino-9-[1,3-bis(isopropoxy)-2-propoxymethyl]purine) was evaluated for its antiviral activity in cell culture and for its therapeutic efficacy in mice infected with herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) or with murine cytomegalovirus (mcmv). hoe 602 was inactive in vitro against a variety of dna- and rna-viruses. however it prevented symptoms and mortality in mice systemically infected with hsv-1, hsv-2 or mcmv when administered intra ... | 1990 | 2177317 |
| [infections caused by viruses and chlamydia trachomatis on uterine cervix]. | a study on the infections caused by hpv-16, hsv-2, cmv and chlamydia trachomatis (ct) on the uterine cervices were carried out among 188 normal cases and 544 patients with chronic cervicitis using elisa, virus isolation and/or dna hybridization methods. the detection rates of these four pathogens in erosive cervices were 23.9% (64/268), 26.5% (99/374), 14.0% (22/157) and 13.4% (18/134), respectively, which were significantly higher than that in normal cervices. the total detection rate of four p ... | 1990 | 2178079 |
| search for herpes simplex virus 2 and human papillomavirus genetic expression in vulvar neoplasia. | specimens from vulvar carcinomas and vulvar intraepithelial neoplasia (vin) of various degrees were analyzed for the presence of herpes simplex virus 2 (hsv 2) and human papillomavirus (hpv) genetic information. a search for the hpv 16 e6 protein as well as the hsv 2 antigenic determinant la1 and icsp 11/12 protein was carried out with an immunoperoxidase assay on 12 vulvar carcinomas and 6 vins. seven invasive cancers and four vins were screened for the presence of homology to hpv 16 dna and hs ... | 1990 | 2178189 |
| double-blind, placebo-controlled trial of a herpes simplex virus type 2 glycoprotein vaccine in persons at high risk for genital herpes infection. | to determine the efficacy of a herpes simplex virus type 2 (hsv-2) glycoprotein subunit vaccine, vaccine (50 micrograms) or placebo was administered intramuscularly at weeks 0, 4, and 22 to 161 persons who lacked hsv-2 antibodies and were sex partners of persons with recurrent genital herpes. the annual rate of acquisition of hsv infection was similar among vaccine and placebo recipients (10.7% and 8%, respectively) but was higher in initially seronegative subjects (15.5%) than in those with hsv ... | 1990 | 2181031 |
| cervical cancer: aetiology, earlier diagnosis and treatment. | cervical cancer which is one of the commonest genital malignancies in women worldwide including singapore is reviewed. there is a decreased incidence and mortality rate from cervical cancer with well organised population-based screening programmes using cervical smears. strong evidence exists for a sexually transmitted etiologic agent in cervical cancer. the evidence of an association between herpes simplex virus type 2 (hsv-2) and certain human papillomavirus (hpv) infections especially hpv 16 ... | 1990 | 2189347 |
| herpes simplex virus type 2 and other genital ulcerative infections as a risk factor for hiv-1 acquisition. | we studied the role of genital ulcerative infections for acquisition of human immunodeficiency virus type 1 (hiv-1) infection in a cohort of 989 homosexual men in amsterdam between october 1984 and december 1988. among 53 hiv-1 seroconverters serological and anamnestic data were gathered regarding herpes simplex virus type 2 (hsv-2) and syphilis in the 6 months before seroconversion. for statistical analysis a control who remained seronegative during the same interval was selected at random for ... | 1990 | 2245979 |
| genital herpes simplex virus infections. | there has been a dramatic increase in patient visits to physicians for evaluation and treatment of genital herpes infections. this has resulted in part from an increase in genital herpes infections, particularly severe, first-episode genital herpes infections in adults without prior hsv-1 infection. virus culture remains the most sensitive and specific method for diagnosis, and use of viral cultures is encouraged. type-specific antibody tests have been employed in studies documenting the role of ... | 1990 | 2246948 |
| ulcerative lesions and herpes simplex virus type 2 in a patient with evan's syndrome. | we have described a case of herpes genitalis in a patient with evans's syndrome who had violaceous macules leading to deep ulcerations. this case serves as a reminder that hsv infection may be atypical and have an unusual course in immunocompromised patients. with the availability of specific therapy for this infection, it is important to consider hsv in the differential diagnosis of all ulcerative skin lesions. | 1990 | 2251541 |
| anesthesia for cesarean delivery in patients with herpes simplex virus type-2 infections. | some concerns exist regarding the use of regional anesthesia for patients with active genital herpes simplex virus type-2 (hsv) infections undergoing cesarean delivery. previous studies have reported the safe use of epidural anesthesia in these patients; there are no reports of the use of spinal anesthesia in this setting. we performed a 6-year retrospective survey of 169 patients delivered by cesarean delivery because of hsv infection. one hundred sixty-four of these patients had the diagnosis ... | 1990 | 2271471 |
| viral infections and recurrences of febrile convulsions. | to determine whether complicated febrile seizures occur more often in children with a proven viral infection, we performed viral examinations on 144 children with febrile convulsions, of whom 112 had simple and 32 had complicated seizures. a diagnosis of virus infection was verified in 46% of the former patients and 53% of the latter. three adenoviruses, one parainfluenza virus type 2 and one type 3, one respiratory syncytial virus, one echovirus type 11, one herpes simplex virus type 2, and one ... | 1990 | 2299489 |
| treatment of resistant herpes simplex virus with continuous-infusion acyclovir. | two patients with acquired immunodeficiency syndrome who developed severe ulcerative proctitis caused by herpes simplex virus type 2 that was resistant to acyclovir were successfully treated with 6 weeks of high-dose, continuous-infusion acyclovir sodium (1.5 to 2.0 mg/kg per hour). viruses cultured from the lesions were resistant to acyclovir in vitro after the patients had received prolonged therapy with oral and intravenous acyclovir in traditional divided doses. investigation into the mechan ... | 1990 | 2308204 |
| is there an association between fetal viral infection and fetal malformation? i. detection of specific igm antibodies in the serum of malformed fetuses. | determinations of igg and igm antibodies specific for cytomegalovirus (cmv), herpes simplex virus (hsv1), herpes simplex virus (hsv2), varicella-zoster virus (vzv), rubella, echo, coxsackie and morbilli viruses were performed in 20 sera from malformed fetuses. demonstration of a fetal infection by increased fetal serum igm permits linkage to a detected fetal malformation. in parallel, 14 maternal sera and 17 amniotic fluid samples were examined. laser nephelometry (a quantitative method) was use ... | 1990 | 2366130 |
| seroepidemiology of chlamydia in costa rica. | a population-based study of the sero-epidemiology of chlamydia was performed among a nationally representative sample of 760 costa rican women aged 25 to 59 years. interviews and sera collection were completed between september 1984 and february 1985. the overall seroprevalence of chlamydial antibodies among these women was 56.1%. women 25 to 39 years of age had a seroprevalence of 51.1%, while women 40 to 59 years of age had a seroprevalence of 64.2%. women who reported no prior sexual activity ... | 1990 | 2370061 |
| physical and biological characterization of antigen presenting liposome formulations: relative efficacy for the treatment of recurrent genital hsv-2 in guinea pigs. | antigen presenting liposomes (apls) containing both liposome encapsulated (44%) and free (56%) recombinant glycoprotein d of herpes simplex virus type-2 (rgd-2) were characterized with respect to the interaction of the antigen with the lipid bilayer and the biological activities provided by each form of rgd-2. we found that free rgd-2 added externally to empty liposomes exhibited some biological activities both in vitro and in vivo, although we could not detect any significant adsorption and/or ... | 1990 | 2383000 |
| daily stress and recurrence of genital herpes simplex. | it is widely believed that emotional stress triggers recurrences of both oral and genital herpes simplex virus. on a daily basis, we studied 64 subjects prospectively for 1 to 3 months; all subjects had at least one culture-proved recurrence of genital herpes simplex virus type 2 before or during the study. all subjects filled out a questionnaire that measured psychological/emotional stress in six areas: physical health, relations with friends, relations with family, relations with sex partner(s ... | 1990 | 2393320 |
| herpes simplex virus infection of the hand. | the first episode of herpes simplex virus (hsv) infection of the hand can occur in association with infection at another site, such as primary hsv-1 gingivostomatitis. autoinoculation is the likely route of transmission. the attacks are usually solitary and last about 14 days. a first episode that results from exogenous inoculation may be severely inflammatory and may last up to 28 days. the majority of cases are recurrent infections and most often occur in adults with hsv-2 infection. | 1990 | 2405025 |
| improved assays for dna-polymerizing enzymes by the use of enzymatically synthesized 5-[125i]iodo-2'-deoxyuridine triphosphate, illustrated by direct quantitation of anti-hiv reverse transcriptase antibody and by serum dna polymerase analyses. | a one-step procedure which uses enzymes in a crude extract of herpes simplex virus (hsv) type 1-infected cells to synthesize 5-[125i]iodo-2'-deoxyuridine triphosphate [( 125i]dutp) from [125i]du is described. the design of a one-step procedure for the purification of the product is also presented. the recovery of [125i]dutp from [125i]du varied between 50 and 75%, the radiochemical purity of the product was greater than 90%, and both synthesis and purification were completed within 8 h. the sens ... | 1990 | 1690011 |
| conserved epitopes of simian herpesvirus sa 8 and bovine herpesvirus type 2. | some major structural components of simian herpesvirus sa 8 were analyzed and the relationship of sa 8 with hsv-1 and especially with bhv-2 was further characterized using a panel of sa 8- and bhv-2-specific monoclonal antibodies directed against gb, gd, ge, and icp 8. it could be shown that sa 8 and bhv-2 expressed gb-1 equivalents, which differ in electrophoretic mobility, but share common epitopes with hsv-1. the antigenic determinants were detectable in the cytoplasm, on the surface of infec ... | 1990 | 1691622 |
| novel membrane-interactive ether lipid analogs that inhibit infectious hiv-1 production and induce defective virus formation. | a new class of membrane-active ether lipid (el) analogs of platelet-activating factor were studied for in vitro anti-hiv-1 activity. human t-cell (cem-ss) monolayers or suspension cultures were used to determine effects of structural modifications of type a phosphorus-containing and type b nonphosphorus el analogs on (a) the inhibitory concentration50 (ic50) for hiv-1 syncytial plaque formation and cell growth, and, (b) virus budding at the cell plasma membrane. results indicate that representat ... | 1990 | 1692729 |
| glycoprotein gb of herpes simplex virus expresses type-common and type-specific antigenic determinants in vivo. | four monoclonal antibodies directed against glycoprotein b of herpes simplex virus were evaluated for their ability to immunize mice passively against acute virus-induced neurological illness and death when administered intraperitoneally 2 hours prior to footpad challenge with type 1 or type 2 virus. two monoclonal antibodies, h120 and h157, failed to reduce the severity of neurological disease in infected animals. in contrast, h233 and h368 antibodies provided significant protection in type-com ... | 1990 | 1692872 |
| selective virus inhibitors. | our recent efforts have been directed at the development of selective inhibitors of different classes of viruses, including adeno, pox, and herpesviruses [herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv)], (+/-)rna viruses (reo- and rotavirus), (-)rna viruses (influenza, parainfluenza, measles, respiratory syncytial, vesicular stomatitis and rabies virus) and retroviruses [i.e. human immunodeficiency virus (hiv), the ... | 1990 | 1693749 |
| role of exogenous interferons on intrinsic antiviral activity of macrophages from patients affected by neoplasia. | macrophages derived from in vitro cultured monocytes were infected with herpes simplex virus type 2. a marked impairment in the intrinsic antiviral activity was found in macrophages obtained from patients with breast cancer or melanoma. moreover, the antiviral activity of macrophages from healthy donors, differentiated in serum from patients with neoplasia, was also impaired. the aim of this work was the evaluation of alpha, beta, gamma exogenous interferon in restoring the intrinsic antiviral a ... | 1990 | 1694541 |
| anti-interferon antibody inhibits natural killer cell activity in hsv-2 genital infection in c57bl/6j mice. | we reported previously that the administration of anti-alpha/beta murine interferon antibody (anti-ifn-ab) to c57bl/6j mice genitally infected with herpes simplex virus type 2 (hsv-2) dramatically increases morbidity and mortality. the effect of anti-ifn-ab is visible early postinfection and it is more evident when low virus doses are used. this report describes our study of the mechanism of action of anti-ifn-ab. we found that anti-ifn-ab did not affect peripheral multiplication of the virus at ... | 1990 | 1695621 |
| comparative therapeutic efficacy of recombinant interferons-alpha, -beta, and -gamma against alphatogavirus, bunyavirus, flavivirus, and herpesvirus infections. | recombinant (r) preparations of interferons (ifn)-alpha, -beta, and -gamma were shown to protect mice against experimental virus infections with herpes simplex virus type 2 (hsv-2), and with three rna-containing viruses from different families: banzi, a flavivirus; semliki forest virus (sfv), an alphatogavirus; and caraparu, a bunyavirus. the antiviral effects of the three different types of ifn were different with each virus. hsv-2 was the most sensitive virus, followed by sfv. against banzi vi ... | 1990 | 1696607 |
| intratypic variation of herpes simplex virus type 2 isolates detected by monoclonal antibodies against viral glycoproteins. | monoclonal antibodies (mabs) to herpes simplex virus (hsv) glycoproteins gd, gg, gb, and ge were used to analyze antigenic variations of 128 genital hsv-2 isolates by an indirect enzyme-linked immunosorbent assay (elisa). isolates were considered significantly different from the standard hsv-2 strain 186 when their optical density (od) in elisa was less than half that of strain 186. this criterion gave 30 patterns of reactivity among the genital hsv-2 isolates. the mabs to gb, gg, and 2 of the g ... | 1990 | 1701081 |
| prevalence of specific antibodies to herpes simplex virus type 2 as revealed by an enzyme-linked immunoassay and western blot analysis. | a solid-phase elisa for the detection of antibodies to gg-2 was developed. the assay utilizes a recombinant dna-derived gg-2 as a solid-phase "capture" reagent and goat anti-human igg (gamma) conjugate to horseradish peroxidase as a probe (detector) reagent. a total of 229 serum samples collected from various populations were tested by elisa and western blot analysis. on comparison with confirmed hsv-2 infection, the sensitivity and specificity of the elisa were 92.9% and 98.7%, respectively. we ... | 1990 | 1963039 |
| recurrent herpetic keratitis: failure to detect herpes simplex virus infection using the syva microtrak hsv1/hsv2 direct specimen identification/typing test. | a 35-year-old man had developed recurrent herpetic keratitis characterized by dendritic keratitis at intervals of a year. we were able to culture cytopathic agents repeatedly from his lesions by inoculating vero cells. the cultures yielded definitive evidence of a virus that caused a cytopathic effect within 3 days. however, these virus strains could not be identified as herpes simplex virus (hsv) in immunofluorescence assays using the syva microtrak hsv1/hsv2 direct specimen identification/typi ... | 1990 | 1963928 |
| incidence of herpes simplex virus type-1 and type-2 from patients with primary (first-attack) genital herpes in sheffield. | in a study to determine the herpes simplex virus (hsv) type isolated from patients with primary (first-attack) genital herpes and to identify any clinical or epidemiological features that might influence the reported incidence of any particular viral type, there was almost an equal incidence of hsv-1 and hsv-2 isolated from women (48% versus 52%). in the case of women, the initial clinical diagnosis was correct in 97% with hsv-1 and 95% with hsv-2 infection. amongst men, hsv-2 was isolated more ... | 1990 | 1964600 |
| clinical and laboratory study of first episode genital herpes in singapore. | a prospective study of the epidemiology, clinical aspects and laboratory features of first episode genital herpes was carried out in middle road hospital, singapore between june 1986 and december 1987. a total of 68 patients were studied. there were more women than men in the study population with a female to male ratio of 7.5 to 1. the peak incidence of first episode genital herpes was between 20 and 29 years of age. specimens from 63 patients yielded positive culture for herpes simplex virus ( ... | 1990 | 1964601 |
| inducible expression of herpes simplex virus type 1 glycoprotein c in nih 3t3 cells. | antibodies directed against the glycoprotein c (gc) of herpes simplex virus type 1 (hsv-1) are known to be mainly hsv-1-specific, whereas antibodies against other major hsv-1 glycoproteins cross-react with hsv-2 antigens. to investigate the immunological features of gc-1, the gene encoding gc-1 was isolated and cloned from dna of cells infected with hsv-1. the 3.6 kbp sali fragment r of hsv-1 dna was modified in order to place the gene under transcriptional control of the glucocorticoid dependen ... | 1990 | 1965287 |
| isolation and typing of herpes simplex viruses in the infections of mouth, respiratory and genital tracts. | a total of 60 herpes simplex virus (hsv) isolates obtained from individuals with genital herpes lesions in the mouth or respiratory illness were typed in this study. the hsv isolation rate in genital herpes was 31%. the typing was done by a direct fluorescent monoclonal antibody technique using commercial reagents. all the 60 vero cell grown isolates were successfully typed without any indeterminate results. nine isolates were typed as hsv 1 and 51 as hsv 2. the technique was easy to perform and ... | 1990 | 1965431 |
| natural killer (nk) cell activity of mice treated with herpes simplex virus type-2, sarcoma-180 cells, cyclosporine and cimetidine. | nk cell activity in beige mice was compared with that in control balb/c mice and balb/c mice treated with hsv-2, sarcoma-180 cells, cimetidine and cyclosporine. the nk cell activity in five week-old beige mice was lower than in three week-old balb/c mice. the nk cell activity in eight week-old balb/c mice was four to eleven times the activity in balb/c mice that were three weeks of age. balb/c mice, injected with 2 mg cyclosporine or 10(7) plaque forming units (pfu) of hsv-2, had decreased nk ce ... | 1990 | 1965843 |
| human immunodeficiency virus type 1 (hiv-1) and herpes simplex virus type 2 (hsv-2) can coinfect and simultaneously replicate in the same human cd4+ cell: effect of coinfection on infectious hsv-2 and hiv-1 replication. | experiments were designed to determine whether hiv-1 and herpes simplex virus type 2 (hsv-2) coinfection leads to simultaneous replication of both viruses in the same human cd4+ cell (mt-4 cell line) and the possible effects of coinfection on infectious virus production. results from transmission electron microscopy analysis revealed replication of typical hsv-2 nucleocapsids in the nucleus and budding of hiv-1 particles through the plasma membrane and through intracytoplasmic vacuoles containin ... | 1990 | 1972888 |
| herpes simplex virus glycoprotein d is recognized as antigen by cd4+ and cd8+ t lymphocytes from infected mice. characterization of t cell clones. | several previous reports have described the surprising inability to detect murine ctl specific for glycoprotein d (gd), one of the important protective immunogens of hsv. using slight variations of published procedures, we were able to show that the immune response to hsv in infected mice includes the generation of ctl specific for gd. c3h/ouj (h-2k) mice were infected by injection in the hind footpads with purified hsv-1. lymphocytes from draining lymph nodes were then isolated and shown to pro ... | 1990 | 1973187 |
| herpes simplex virus antigens and inflammatory cells in oral lesions in recurrent erythema multiforme. immunoperoxidase and autoradiographic studies. | herpes simplex virus (hsv) antigens were sought in 15 biopsy specimens from both lesional mucosa and clinically healthy looking oral mucosa between attacks in patients with erythema multiforme (em). four of the eight biopsy specimens obtained from lesional em mucosa stained positively with hsv-1-and/or hsv-2-specific antisera applied in direct immunoperoxidase staining. of the 16 tissue specimens used as controls, two displayed positive staining with hsv-1 and/or hsv-2. five of the seven biopsy ... | 1990 | 1980974 |
| identification of an infectious laryngotracheitis virus equivalent to the herpes simplex virus type 2 major dna binding protein (icp8). | a region of homology between the genomic dna of infectious laryngotracheitis virus (iltv), an avian herpesvirus, and the region encoding the herpes simplex virus type 2 (hsv-2) major dna binding protein (icp8) has been detected by the use of nick translation and southern blot analysis. further, a monoclonal antibody directed against the hsv-2 icp8 protein detected has antigenic cross-reaction with iltv as demonstrated by indirect immunofluorescence. | 1990 | 1981454 |
| equine herpes virus 1 and pseudorabies virus resistance to 2'-fluoropyrimidine analogs and to bromovinyldeoxyuridine: implications for dtmp kinase activity. | the 2'-fluoropyrimidine nucleoside analogs 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac). 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), and 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau) showed higher in vitro activity against herpes simplex virus type 1 (hsv-1), than equine herpesvirus 1 (ehv-1) or pseudorabies virus (prv). comparison of the 50% plaque inhibitory doses for hsv-1 and its mutant mmdur-20 in cell cultures with inhibition c ... | 1990 | 1983184 |
| evolution of post-natal herpes simplex virus encephalitis to multicystic encephalopathy. | a 3-week-old, previously healthy infant developed biopsy-proven herpes virus type 2 (hsv-2) encephalitis. the encephalitis was characterized by cells having intranuclear inclusions and was without evidence of inflammation or hemorrhage. neuroimaging studies did not show any destructive lesions in the brain. in spite of antiviral therapy, the infant's neurological conditions deteriorated, and the patient died at the age of 18 weeks. post-mortem examination showed that most of the cerebral hemisph ... | 1990 | 1703387 |
| [pemphigus vulgaris superinfected by herpes simplex virus ii in the absence of corticosteroid therapy]. | 1991 | 1826364 | |
| colonization of adrenal glands and ovaries of mice by variants of hsv 1 and 2. ii. histopathological, immunohistochemical and in situ hybridization studies. | the herpes simplex virus (hsv)-infected mouse model was used to correlate histopathological lesions in adrenal glands and ovaries with the localisation of viral nucleic acids and viral antigens, employing in situ hybridization and immunohistochemistry. in the adrenals, the lesions were mainly restricted to the zona fasciculata and the zona reticularis, sometimes extending to the medulla. in the ovaries, lesions were detected in follicles and in the stroma. during the course of infection, hsv nuc ... | 1991 | 2018430 |
| expression in bacteria of a polypeptide encoded by a transforming fragment of herpes simplex virus type 2. | we have constructed a plasmid (pmd2) containing the 38,000 mw polypeptide (38k polypeptide) gene from the transforming bg1ii-n fragment of hsv-2 fused to the amino-terminal portion of the beta-galactosidase gene in plasmid puc8. nucleotide sequence determination around the fusion-junction confirmed that the viral gene sequences starting at its second codon is in the correct reading frame in relation to the translation initiation codon of beta-galactosidase. the lac control sequences direct the s ... | 1991 | 2032251 |
| [massive necrotizing adenitis complicating a disseminated herpes simplex virus 2 infection in chronic lymphoid leukemia]. | we report a case of a 68 years old male patient treated for chronic lymphocytic leukaemia, presenting a right inguino-crural tender lymphadenopathy with periadenitis. the histologic changes associated with adenitis were large foci of necrosis with regular crown of epithelioid cells, without giant cell. the presence of herpes simplex virus 2 was confirmed by immunohistochemistry and culture. occurrence, physiopathology, clinical and histological aspects of herpes simplex lymphadenitis in patients ... | 1991 | 2036132 |
| synthesis and antiviral activity of 5-heteroaryl-substituted 2'-deoxyuridines. | the synthesis of 5-heteroaryl-substituted 2'-deoxyuridines is described. the heteroaromatics were obtained from three different 5-substituted 2'-deoxyuridines. cycloaddition reaction of nitrile oxides on the 5-ethynyl derivative 1 gave the isoxazoles 4a-e. the thiazole derivatives 14a-c were obtained from the 5-thiocarboxamide 11, while 5-pyrrol-1-yl-2'-deoxyuridine (17) could be synthesized directly from 5-amino-2'-deoxyuridine. the compounds were evaluated for antiviral activity. selective act ... | 1991 | 2061920 |
| efficacy of (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine in various models of herpes simplex virus infection in mice. | the phosphonylmethoxyalkyl derivative (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc) was evaluated for its in vivo efficacy in several model infections for herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and thymidine kinase-deficient (tk-) hsv-1 in mice. in hairless mice infected intracutaneously with hsv-1 or hsv-2, hpmpc completely suppressed all manifestations of the disease (skin lesions, paralysis of the hind legs, and mortality) if it was administered topically at a co ... | 1991 | 2069375 |
| three cell lines from hamster buccal pouch tumors induced by topical 7,12-dimethylbenz(a)anthracene, alone or in conjunction with herpes simplex virus inoculation. | three squamous carcinoma cell lines hbpc-1, hbpc-2, and hbpc-3 were established from hamster buccal pouch tumors induced by topical 7,12-dimethylbenz(a)anthracene (dmba) treatment alone, topical dmba treatment in conjunction with type 1 herpes simplex virus (hsv-1) inoculation, and topical dmba application in combination with type 2 hsv (hsv-2) inoculation, respectively. the cells were epithelial in morphology, had a doubling time of approximately 18 h, and required bovine serum for optimal grow ... | 1991 | 1708371 |
| a follow-up study of children with neonatal herpes simplex virus infections with particular regard to late nervous disturbances. | forty-five children with neonatal herpes simplex virus (hsv) infection, representing all known cases in the diagnostic records of four virological laboratories within a 15-year period, were followed up. twelve children had died. sixteen of the 33 survivors were healthy. thirteen children had severe disabilities: all of them showed severe mental retardation; moreover, 11 were tetraplegic, one was hemiplegic with hydrocephalus and one had a pronounced behavioural abnormality. four children had sli ... | 1991 | 1709771 |
| a related epitope is consistently present on glycoprotein c of herpes simplex virus type 1 and 2. | a monoclonal antibody (ve8) directed to glycoprotein c of herpes simplex virus type 1 (hsv-1) cross-reacted with all hsv type 2 (hsv-2) strains tested. positive reaction was also observed with all investigated hsv-1 strains, indicating that the related epitope is consistently present in hsv-1 and hsv-2. | 1991 | 1719785 |
| intratypic variations in neutralizable epitopes among herpes simplex virus type 2 isolates. | intratypic variation among 94 isolates of herpes simplex virus type 2 (hsv-2) was investigated using 4 different monoclonal antibodies (mabs). by neutralization test, these mabs appeared to be directed to at least 2 distinct epitopes on the viral glycoprotein d (gd), i.e., 6g6.g9 and 6e8.f11 which did not require complement (c-mab) and gd-105 and gd-110 whose neutralizing activities could be enhanced by complement (c+mab). the c-mab pairs each separately could detect significant intratypic varia ... | 1991 | 1723784 |
| computer predictions of antigenic domains in herpes simplex virus types 1 and 2 glycoprotein d as compared with experimentally proven domains. | the primary amino-acid sequence of the glycoprotein d (gd) of herpes simplex virus types 1 and 2 (hsv-1, hsv-2) was analyzed by computer programs that provided values for hydrophilicity, surface probability, flexibility, and antigenicity, as well as the secondary structure conformation. putative antigenic domains with a high hydrophilicity, surface probability, and antigenicity index were determined and compared with the reported antigenic domains in hsv-1 and hsv-2 gd protein based on experimen ... | 1991 | 1724582 |
| herpes simplex virus infection of the placenta. the role of molecular pathology in the diagnosis of viral infection of placental-associated tissues. | herpes simplex infections of the placenta and its associated tissues are rarely seen and, consequently, the pathologic features are not well understood. we describe the occurrence of herpes simplex virus infection of the placenta that was limited to maternal-derived cells of the subchorionic tissue. the microscopic findings were remarkable for both the absence of inflammation and characteristic viral inclusions. however, in situ dna hybridization, as well as immunohistochemistry using antibody t ... | 1991 | 1747033 |
| effect of dsrna from phi 6 bacteriophage on herpetic infection in cell culture and an animal model. | the double-stranded (ds) rna from phi 6 bacteriophage inhibited herpes simplex virus type 1 (hsv-1) and hsv-2 infection in ma-104 cells but not in vero cells. hsv-2 was more sensitive to this effect than hsv-1, with the hsv-2 ed50 being 0.25 micrograms/ml and the hsv-1 ed50 1.68 micrograms/ml. on genital infection by hsv-2 in guinea pigs, phi 6 dsrna was more effective by intravaginal (p less than 0.05) than by intraperitoneal administration. a single dose of dsrna of 600 micrograms/kg by intrav ... | 1991 | 1774466 |
| [esophageal pathology in patients with the aids virus. etiology and diagnosis]. | from 180 patients infected with human immunodeficiency virus (hiv) and followed-up for one year, 17 cases (9.44%) were referred to detect oesophageal pathology. they were prospectively analyzed through fibroscopy, radiology, biopsies for histopathology, virology and mycology and brush cytology. most frequent symptoms were dysphagia. odynophagia and retrosternal pain, usually associated, and not providing an accurate diagnostic clue. the most common causes of symptoms were oesophageal candidiasis ... | 1991 | 1820692 |
| selective depletion of liver and splenic macrophages using liposomes encapsulating the drug dichloromethylene diphosphonate: effects on antimicrobial resistance. | the current results provide direct evidence for a role of tissue macrophages (m phi) in natural immunity and support the use of immunomodulators to enhance antiviral resistance in immunocompromised individuals. in this study, macrophages (m phi) in the spleen and liver were eliminated by intravenous (i.v.) injection of the drug dichloromethylene diphosphonate (dmdp) encapsulated in liposomes. the effect of this depletion system on peritoneal m phi, peripheral blood leukocytes, splenic natural ki ... | 1991 | 1827490 |
| herpes simplex virus type-2 ulcers resistant to acyclovir in an aids patient--successful treatment with foscarnet. | the case of a 25-year-old homosexual man with large inguinal and perianal ulcers is reported. he had been pretreated extensively with acyclovir. herpes simplex virus (hsv) was identified in the ulcer tissue by in-situ hybridization. no clinical improvement with acyclovir but a prompt response to foscarnet was noted. in a relapse after 11 weeks an acyclovir-resistant hsv type 2 was isolated. again, a prompt response to foscarnet was noted. the case is presented in detail, and the clinical impact ... | 1991 | 1828096 |
| acyclovir resistance/susceptibility in herpes simplex virus type 2 sequential isolates from an aids patient. | the biological characterization of a number of sequential herpes simplex virus type 2 (hsv-2) isolates obtained from an aids patient undergoing sequential courses of antiviral treatment due to an extended mucocutaneous genital lesion is reported. resistance to acyclovir (acv) and related compounds was linked to a thymidine kinase-deficient (tk-) phenotype. after acv discontinuation and a course of treatment with foscarnet, a new isolate was recovered, characterized by loss of the acv-resistant t ... | 1991 | 1832460 |
| a microbiological study of genital ulcers in kuala lumpur. | the microbial aetiology of genital ulcers was studied in 249 patients (241 men and 8 women) attending a sexually transmitted disease clinic in kuala lumpur, malaysia. herpes simplex virus type 2 was isolated in 48 (19.2%) patients, haemophilus ducreyi from 22 (8.8%), neisseria gonorrhoeae from seven (2.8%) and chlamydia trachomatis from four (1.6%). syphilis was diagnosed in 18 (7.2%) patients on the basis of dark field microscopy. two (0.8%) patients were found to have both chancroid and syphil ... | 1991 | 1839925 |
| characterization of herpes simplex virus type 2 latency-associated transcription in human sacral ganglia and in cell culture. | the ability of herpes simplex virus type 2 (hsv-2) to establish latency in and reactivate from sacral dorsal root sensory ganglia is the basis for recurrent genital herpes. the expression of hsv-2 genes in latently infected human sacral ganglia was investigated by in situ hybridization. hybridizations with a probe from the long repeat region of hsv-2 revealed strong nuclear signals overlying neurons in sacral ganglia from five of nine individuals. the rna detected overlaps with the transcript fo ... | 1991 | 1845807 |
| type-specific identification of herpes simplex and varicella-zoster virus antigen in autopsy tissues. | to identify antigens of herpes simplex virus (hsv) types 1 and 2 and varicella-zoster virus (vzv) in human tissue, polyclonal antisera and an immunoperoxidase method were used to examine formalin-fixed, paraffin-embedded tissues from autopsy cases and experimentally infected animals. these antisera readily distinguished between hsv and vzv antigen, with no evident cross-reactivity. antiser ato hsv-1 and hsv-2 were more strongly reactive with antigen of the homologous virus than with that of hete ... | 1991 | 1845866 |
| the promoter, transcriptional unit, and coding sequence of herpes simplex virus 1 family 35 proteins are contained within and in frame with the ul26 open reading frame. | the herpes simplex virus 1 (hsv-1) genome specifies an abundant capsid protein which in denaturing gels forms multiple bands designated family 35 proteins (d.k. braun, b. roizman, and l. pereira, j. virol. 49:142-153, 1984). nucleotide-sequencing studies have assigned the coding sequences of these proteins to the open reading frame ul26 (d.j. mcgeoch, m.a. dalrymple, a.j. davidson, a. dolan, m.c. frame, d. mcnab, l.j. perry, j.e. scott, and p. taylor, j. gen. virol. 69:1531-1574, 1988). in studi ... | 1991 | 1845885 |
| mechanism and mode of action of 5-iodo-2-pyrimidinone 2'-deoxyribonucleoside, a potent anti-herpes simplex virus compound, in herpes simplex virus-infected cells. | the anti-herpes simplex virus type 2 (-hsv-2) action of 5-iodo-2-pyrimidinone deoxyribonucleoside (ipdr) was found to be exerted through inhibition of hsv dna synthesis. the inhibition of viral dna synthesis was not caused by inhibition of the synthesis of hsv-2-specified proteins or hsv-2 mrna species involved with viral dna synthesis or by depletion of deoxynucleotides. the inhibition of viral dna synthesis may be due to damage to the dna template in the nuclei or to an action at the dna repli ... | 1991 | 1846219 |
| herpes simplex virus (hsv) glycoprotein h is partially processed in a cell line that expresses the glycoprotein and fully processed in cells infected with deletion or ts mutants in the known hsv glycoproteins. | cell lines that constitutively express herpes simplex virus 1 (hsv-1) glycoprotein h (gh-1) failed to synthesize the mature form of gh and accumulated a precursor-like form of the glycoprotein, which was retained intracellularly, most likely in rer. fine-structure analysis of the oligosaccharides present in recombinant gh revealed oligosaccharides processed by rer enzymes; sialylated complex-type and biantennary oligosaccharides, which are assembled in the trans-golgi, were absent. a small fract ... | 1991 | 1846486 |
| synthesis and antiviral activity of 9-alkoxypurines. 2. 9-(2,3-dihydroxypropoxy)-, 9-(3,4-dihydroxybutoxy)-, and 9-(1,4-dihydroxybut-2-oxy)purines. | reaction of alkenoxyamines (3,5) or (r,s)-, (r)-, and (s)-hydroxy-protected derivatives of hydroxyalkoxyamines (20a,b, 37a-c) with 4,6-dichloro-2,5-diformamidopyrimidine (4) and cyclization of the resultant 6-[(alkenoxy)amino]-and 6-(alkoxyamino)pyrimidines (6,7,21a,b, 38a,b,c) by heating with diethoxymethyl acetate afforded 9-alkenoxy- and 9-alkoxy-6-chloropurines (9,10,22a,b, 39a-c, 40a). these were subsequently converted to 9-(2,3-dihydroxypropoxy), 9-(3,4-dihydroxybutoxy), and 9-(1,4-dihydro ... | 1991 | 1846922 |
| latent herpes simplex virus reactivation in the guinea pig. an animal model for recurrent disease. | the purpose of this investigation was to establish an animal model by which latent herpetic disease could be mechanically reactivated, yielding an adequate number of recurrent clinical lesions in the guinea pig. to determine strain virulence, hairless guinea pigs were inoculated with three different strains of herpes simplex virus (hsv) using a spring-loaded multiple puncture apparatus. hsv-1 strains sc-16 and mckrae produced an average of 21 and 8 lesions per infected area, respectively. the hs ... | 1991 | 1847124 |
| the difference in sensitivity to cicloxolone sodium between herpes simplex virus types 1 and 2 maps to the locations of genes ul22 (gh) and ul44 (gc). | cicloxolone sodium (ccx) is a broad spectrum antiviral agent which has a largely non-specific and complex mode of antiviral action. however, the experimental finding that herpes simplex virus type 2 (hsv-2) (strain hg52) is consistently more sensitive to inhibition by ccx than hsv-1 (117 syn+) additionally implies the specific involvement of hsv genes. hsv-1/hsv-2 intertypic recombinants have been utilized to investigate this genetic difference by comparing their ccx ed50 concentrations. no shor ... | 1991 | 1847174 |
| construction of herpes simplex viruses that are pseudodiploid for the glycoprotein b gene: a strategy for studying the function of an essential herpesvirus gene. | the primary structure of glycoprotein b (gb) is conserved strongly among many members of the herpesviridae, including some that differ vastly in their natural properties. to determine whether the structural similarity between the gbs of herpes simplex virus type 1 (hsv-1) and bovine herpesvirus type 1 (bhv-1) was reflected in functional homology, we constructed pseudodiploid hsv-1 virions which, in addition to their own gene encoding gb, also contained a gene for encoding bhv-1 gb. two kinds of ... | 1991 | 1847175 |
| the nucleotide sequence of the glycoprotein gb gene of infectious laryngotracheitis virus: analysis and evolutionary relationship to the homologous gene from other herpesviruses. | a 3698 bp region of the genome of infectious laryngotracheitis virus (iltv) was sequenced and found to contain the entire glycoprotein gb gene and the c-terminal region of a gene homologous to the icp 18.5 protein gene of herpes simplex virus type 1. the iltv gb gene encoded a protein with an mr of 100k possessing all the characteristics of a transmembrane glycoprotein. alignment of the iltv gb sequence with homologous sequences from six other herpesviruses revealed that 10 cysteine residues on ... | 1991 | 1847176 |
| the conserved dna-binding domains encoded by the herpes simplex virus type 1 icp4, pseudorabies virus ie180, and varicella-zoster virus orf62 genes recognize similar sites in the corresponding promoters. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), pseudorabies virus (prv), varicella-zoster virus (vzv), and equine herpesvirus 1 (ehv-1) are all classified as alphaherpesvirinae. each of these five viruses encodes an essential immediate-early (ie) regulatory protein referred to as hsv-1 icp4, hsv-2 icp4, prv ie180, vzv orf62 protein, and ehv-1 ie1, respectively. these five proteins share extensive homology with each other in domains referred to as regions 2 and 4. the hsv-1 icp4 region 2 d ... | 1991 | 1847444 |
| identification of an immunodominant cytotoxic t-lymphocyte recognition site in glycoprotein b of herpes simplex virus by using recombinant adenovirus vectors and synthetic peptides. | cytotoxic t-lymphocyte (ctl) responses to herpes simplex virus (hsv) polypeptides play an important role in recovery from infection and in preventing latency. we have previously shown that glycoprotein b (gb) is a major target recognized by hsv-specific ctls in c57bl/6 (h-2b) and balb/c (h-2d) mice but not in cba/j (h-2k) mice (l. a. witmer, k. l. rosenthal, f. l. graham, h. m. friedman, a. yee, and d. c. johnson, j. gen. virol. 71:387-396, 1990). in this report, we utilize adenovirus vectors ex ... | 1991 | 1847447 |
| fluorocarbocyclic nucleosides: synthesis and antiviral activity of 2'- and 6'-fluorocarbocyclic 2'-deoxy guanosines. | a series of four isomeric 2'- and 6'-fluorocarbocyclic guanosine analogues have been prepared and evaluated as potential anti-herpes agents. the racemic 2' beta-fluoro isomer 2-amino-1,9-dihydro- 9-[(1 alpha, 2 alpha, 3 beta, 4 alpha)-2-fluoro-3-hydroxy-4- (hydroxymethyl)cyclopentyl]-6h-purin-6-one (11a, c-afg) and its 2' alpha-fluoro epimer 11b plus the chiral 6' beta-fluoro isomer 2-amino-1,9-dihydro-9-[[1s-(1 alpha, 2 alpha, 3 alpha, 4 beta)]- 2-fluoro-4-hydroxy-3-(hydroxymethyl)cyclopentyl]- ... | 1991 | 1848298 |
| human papillomavirus types 16 and 33, herpes simplex virus type 2 and other risk factors for cervical cancer in sichuan province, china. | cancer of the cervix is relatively common in china, but has been investigated epidemiologically in only a few studies. in the hospital-based case-control study reported here, we investigated the role of various lifestyle and dietary factors, as well as infection with human papillomavirus (hpv) types 16 and 33 and herpes simplex virus type 2 in the aetiology of invasive cervical cancer. the study was conducted in sichuan province, and involved 101 cases with histologically-confirmed cervical canc ... | 1991 | 1848537 |
| herpes simplex virus type 1 deletion variants 1714 and 1716 pinpoint neurovirulence-related sequences in glasgow strain 17+ between immediate early gene 1 and the 'a' sequence. | dideoxynucleotide sequence analysis of a spontaneously isolated deletion variant (1714) of glasgow strain 17+ of herpes simplex virus type 1 (hsv-1) demonstrates that the deletion is 759 bp in length and is located within each copy of the bamhi s fragment (0 to 0.02 and 0.81 to 0.83 map units) of the long repeat region of the genome. the deletion removes one complete copy of the 18 bp dr1 element of the 'a' sequence and terminates 1105 bp upstream of the 5' end of immediate early (ie) gene 1. th ... | 1991 | 1848598 |