Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| vaccine candidates derived from a novel infectious cdna clone of an american genotype dengue virus type 2. | a dengue virus type 2 (den-2 tonga/74) isolated from a 1974 epidemic was characterized by mild illness and belongs to the american genotype of den-2 viruses. to prepare a vaccine candidate, a previously described 30 nucleotide deletion (delta30) in the 3' untranslated region of den-4 has been engineered into the den-2 isolate. | 2004 | 15461822 |
| trans-packaged west nile virus-like particles: infectious properties in vitro and in infected mosquito vectors. | a trans-packaging system for west nile virus (wnv) subgenomic replicon rnas (reprnas), deleted for the structural coding region, was developed. wnv reprnas were efficiently encapsidated by the wnv c/prm/e structural proteins expressed in trans from replication-competent, noncytopathic sindbis virus-derived rnas. infectious virus-like particles (vlps) were produced in titers of up to 10(9) infectious units/ml. wnv vlps established a single round of infection in a variety of different cell lines w ... | 2004 | 15479801 |
| [tyrophagus putrescentiae predating adult insects of aedes aegypti and aedes albopictus in laboratory]. | the present study aimed at identifying a mite infesting a colony of aedes aegypti and ae. albopictus as well as investigating the source of infestation. the mite species was identified after it was mounted on slides in hoyer's medium and examined under an optical microscope. it showed to be tyrophagus putrescentiae. fish and rabbit food samples were kept in an environmental chamber at 27 degrees c and 90% humidity for 21 days and were weekly examined. the presence of t. putrescentiae was only ob ... | 2004 | 15499448 |
| dengue emergence and adaptation to peridomestic mosquitoes. | phylogenetic evidence suggests that endemic and epidemic dengue viruses (denv), transmitted among humans by the anthropophilic mosquitoes aedes aegypti and ae. albopictus, emerged when ancestral, sylvatic denv transmitted among nonhuman primates by sylvatic aedes mosquitoes adapted to these peridomestic vectors. we tested this hypothesis by retrospectively examining evidence for adaptation of epidemic and endemic versus sylvatic strains of denv-2 to ae. albopictus and ae. aegypti. first and seco ... | 2004 | 15504265 |
| markedly reduced severity of dengue virus infection in mosquito cell cultures persistently infected with aedes albopictus densovirus (aaldnv). | aaldnv-infected c6/36 cells serially passaged for over 10 weeks showed a decline in percentage of anti-aaldnv-positive cells (apc) from an initial 92% to approximately 20%. cultures of persistent apc were indistinguishable from uninfected cultures by direct microscopy but most stained cells from early apc passages had enlarged nuclei with eosinophilic inclusions, while late apc passages had few and naive cells none. super challenge of persistent apc cultures did not increase percentage apc and s ... | 2004 | 15518806 |
| entomological observations on dengue vector mosquitoes following a suspected outbreak of dengue in certain parts of nagaland with a note on their susceptibility to insecticides. | three species of aedes viz., aedes albopictus, aedes aegypti and aedes annandalei were detected from different breeding sources in and around human habitats during entomological study conducted following an outbreak suspected to be of dengue (which occurred during, 1994) in parts of medziphema phc area of nagaland in two different points of time ie., in the year, 1994 and, 2000. the potential dengue vector, aedes albopictus showed high preponderance by breeding in all types of containers searche ... | 2004 | 15529881 |
| commercial mosquito trap and gravid trap oviposition media evaluation, atlanta, georgia. | field trials evaluating the effectiveness of selected gravid trap oviposition media and commercially available mosquito traps were conducted in southern fulton county (atlanta), ga, from june 9 to june 18 and june 24 to july 4, 2002, respectively. total number of mosquitoes and number of each species captured during the tests were compared using a latin square design. for the gravid trap infusion media, significant differences were found for total number of mosquitoes collected where sod > or = ... | 2004 | 15532919 |
| yeast-generated co2 as a convenient source of carbon dioxide for adult mosquito sampling. | a new, convenient method was developed to supply co2 for mosquito sampling by using yeast, which converts sugar into co2 and ethyl alcohol. the system could, at average, generate 32.4 ml/min of co2 for at least 27 h. the total weight of the co2 generated was estimated to be 94 g. the efficacy of yeast-generated co2 as attractant for mosquitoes was significant, and the following 6 mosquito species were collected using yeast-generated co2 traps from july to september 2003 in a residential area of ... | 2004 | 15532924 |
| cocoa pod (theobroma caco)--a potential breeding habit of aedes albopictus in dengue-sensitive kerala state, india. | aedes (stegomyia) albopictus (skuse), a potential dengue vector, particularly in the foot-hill areas of the western ghat region of kerala state has been found breeding for the first time in cocoa pods (theobroma cacao) in india. breeding in cocoa pods is considered an opportunistic adaptive behavior of the mosquito to maintain density in the absence of common breeding sites, especially latex-collecting cups in rubber plantations, during the monsoon periods. vector breeding in cocoa pods was obse ... | 2004 | 15532938 |
| susceptibility of ochlerotatus trivittatus (coq.), aedes albopictus (skuse), and culex pipiens (l.) to west nile virus infection. | the susceptibility of ochlerotatus trivittatus (coq.) to west nile virus (wnv) was assessed by comparing it to the susceptibility of aedes albopictus (skuse), a likely bridge vector, and culex pipiens (l.), a primary wnv amplifying species. the three species were infected with wnv (ny crow-1999) by feeding on 2-3-day-old chickens with serum virus titers ranging from 10(2.5) to 10(9.5) cell culture infective dose (cid) 50s/ml. the lowest infective titer for oc. trivittatus and cx. pipiens was 10( ... | 2004 | 15631062 |
| surveillance of dengue vector at thiruvananthapuram (kerala) international airport. | a survey of aedes (stegomyia) mosquitoes was undertaken in and around the international airport at thiruvananthapuram during premonsoon season. an attempt has been made in this paper to elaborate on the presence of risk potential for dengue /yellow fever at the airport with the detection of aedes breeding. the entomological indices have also been discussed with regard to presence of potential wet containers and rate of infestation by aedes mosquitoes. the airport area was searched for aedes (ste ... | 2004 | 16295677 |
| malaria parasite developmental analyses by the nested polymerase chain reaction method: an implication for the evaluation of mosquito infection rates in epidemiological studies. | a malaria mosquito vector, anopheles saperoi, and a non-vector, aedes albopictus, were allowed to feed on mice infected with murine malaria, plasmodium yoelii nigeriensis, and were subsequently monitored for the development of parasites by the nested polymerase chain reaction (pcr) method, using plasmodium genus-specific primer pairs. the mosquitos were divided into two parts, head/thorax and abdomen, for dna analyses. the parasite dna and murine dna for each mosquito were examined in parallel. ... | 2004 | 15916075 |
| mosquito vector control and biology in latin america--a fourteenth symposium. abstracts. | the 14th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 70th annual meeting in savannah, ga, in february 2004. the principal objective, as for the previous 13 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 37 presentations that were given orally in spanish or presented as posters by participant ... | 2004 | 15669378 |
| a comparison of seven traps used for collection of aedes albopictus and aedes aegypti originating from a large tire repository in harris county (houston), texas. | among 7 traps tested, significantly higher (p < 0.01) mean numbers of aedes albopictus (269) and aedes aegypti (55) females were collected within the mosquito magnet liberty trap compared with the remaining traps. the second highest mean captures for both species were obtained from omnidirectional fay-prince (77 ae. albopictus) and dragonfly (13 ae. aegypti) traps, which were not significantly different (p > 0.01) from an experimental moving-target trap that produced mean captures of 40 ae. albo ... | 2004 | 15669373 |
| photoelectric sensing device for recording mosquito host-seeking behavior in the laboratory. | a new automatic device for recording the host-seeking behavior of mosquitoes was designed using photoelectric sensors. the host-seeking rhythms of several species of mosquitoes were recorded under laboratory conditions. use of co2, in addition to heating and black color to activate mosquito flight, proved to be important for evaluation of the present recording device. the diel rhythms of nonblood-fed aedes albopictus (skuse), aedes aegypti (l.), culex quinquefasciatus say, culex tritaeniorhynchu ... | 2004 | 15535615 |
| efficacy of methyl bromide fumigation and insecticidal dips against aedes eggs on lucky bamboo. | importation of lucky bamboo (dracaena sp.) stalk clusters has led to the accidental importation of aedes albopictus in some countries. current methods of fumigation using methyl bromide are harmful to live plants and thus are not acceptable. we exposed aedes aegypti eggs to methyl bromide at rates of 32 g/m3 for 2 and 3 h and 48 g/m3 for 3 h, achieving complete control in 0/5, 1/5, and 5/5 replicates, respectively. submersion for 1 min in aqueous solutions of 0.04% active ingredient (ai) lambda- ... | 2004 | 15532928 |
| alkalization of larval mosquito midgut and the role of carbonic anhydrase in different species of mosquitoes. | we have previously demonstrated the involvement of carbonic anhydrase (ca) in the alkalization mechanism of the aedes aegypti larval midgut. in this study, we used hansson's histochemistry to examine the distribution of the enzyme in the midgut of six different species of mosquito larvae (aedes aegypti, aedes albopictus, culex quinquefasciatus, culex nigripalpus, ochlerotatus taeniorhynchus, anopheles quadrimaculatus). additionally, we quantitated ca content in the gastric caeca, anterior and po ... | 2004 | 15171946 |
| [productivity of aedes albopictus' breeding containers in paraíba valley, brazil]. | to estimate the potential productivity of artificial, permanent, and natural breeding containers of aedes albopictus, potential vector species of dengue. | 2004 | 15122376 |
| a field test for competitive effects of aedes albopictus on a. aegypti in south florida: differences between sites of coexistence and exclusion? | we tested whether interspecific competition from aedes albopictus had measurable effects on a. aegypti at the typical numbers of larval mosquitoes found in cemetery vases in south florida. we also tested whether the effect of interspecific competition from a. albopictus on a. aegypti differed between sites where a. aegypti either persists or went extinct following invasion by a. albopictus. similar experiments manipulating numbers of a. albopictus in cemetery vases were conducted at three sites ... | 2004 | 15024640 |
| high-level gene expression in aedes albopictus cells using a baculovirus hr3 enhancer and ie1 transactivator. | aedes aegypti is the key vector of both the yellow fever and dengue fever viruses throughout many parts of the world. low and variable transgene expression levels due to position effect and position effect variegation are problematic to efforts to create transgenic laboratory strains refractory to these viruses. transformation efficiencies are also less than optimal, likely due to failure to detect expression from all integrated transgenes and potentially due to limited expression of the transpo ... | 2004 | 15251037 |
| habitat complexity and sex-dependent predation of mosquito larvae in containers. | studies in aquatic systems have shown that habitat complexity may provide refuge or reduce the number of encounters prey have with actively searching predators. for ambush predators, habitat complexity may enhance or have no effect on predation rates because it conceals predators, reduces prey detection by predators, or visually impairs both predators and prey. we investigated the effects of habitat complexity and predation by the ambush predators toxorhynchites rutilus and corethrella appendicu ... | 2005 | 16041612 |
| competitive outcomes of aquatic container diptera depend on predation and resource levels. | resources and predation are both known to be important in structuring communities; however the strength of one factor may be affected by the intensity of the other. this study used a fully crossed factorial experiment in laboratory microcosms to examine the ability of a predator, corethrella appendiculata (grabham), and basal resources (leaf litter) to differentially affect two competing species of mosquito prey. increased resources resulted in shorter developmental time and increased survivorsh ... | 2005 | 22661766 |
| oviposition deterrent and skin repellent activities of solanum trilobatum leaf extract against the malarial vector anopheles stephensi. | the leaf extract of solanum trilobatum (solanaceae) was tested under laboratory conditions for oviposition deterrent and skin repellent activities against the adult mosquito anopheles stephensi. concentrations of 0.01, 0.025, 0.05, 0.075 and 0.1% reduced egg laying by gravid females from 18 to 99% compared to ethanol-treated controls. in skin repellent tests, concentrations of 0.001, 0.005, 0.01, 0.015, and 0.02 % provided 70 to 120 minutes protection against mosquito bites, whereas the ethanol ... | 2005 | 16341247 |
| insecticide resistance development in culex quinquefasciatus (say), aedes aegypti (l.) and aedes albopictus (skuse) larvae against malathion, permethrin and temephos. | laboratory-bred females of culex quinquefasciatus, aedes aegypti and aedes albopictus from the insectarium, unit of medical entomology, institute for medical research were used in the experiment. the late third stage of the f0 larvae which survived the high selection pressure of malathion, permethrin and temephos were reared and colonies were established from adults that emerged. cx. quinquefasciatus larvae were subjected to selection by malathion and permethrin for 40 generations, ae. aegypti l ... | 2005 | 16880753 |
| carbonic anhydrase in the adult mosquito midgut. | we have previously demonstrated the involvement of carbonic anhydrase (ca) in larval mosquito midgut physiology. in this study, we used hansson's histochemistry to examine the distribution of the enzyme in the midgut of aedes aegypti, aedes albopictus, culex quinquefasciatus, culex nigripalpus, ochlerotatus taeniorhynchus, anopheles albimanus and anopheles quadrimaculatus adult mosquitoes. additionally, we quantitated ca content in the anterior and posterior midgut of adult males and females fro ... | 2005 | 16109888 |
| comparative study on nocturnal behavior of aedes aegypti and aedes albopictus. | nocturnal behavior of nonblood-fed females of aedes aegypti (l.) and aedes albopictus (skuse) was studied using an automatic recording device equipped with a photoelectric sensor. carbon dioxide, heating, and the contrast of the black and white colors were used as attractive cues for mosquitoes. the nocturnal host-seeking activity positively correlated with the increasing light intensity in both species. ae. aegypti was found to be more sensitive to light than ae. albopictus. the threshold of li ... | 2005 | 15962780 |
| differential identification of ascogregarina species (apicomplexa: lecudinidae) in aedes aegypti and aedes albopictus (diptera: culicidae) by polymerase chain reaction. | we report 2 polymerase chain reaction (pcr)-based methods for distinguishing morphologically similar gregarine species based on amplification of variable regions of the internal transcribed spacer region of ribosomal dna. the gregarines we investigated were ascogregarina barretti (vavra), a. culicis (ross), and a. taiwanensis (lien and levine), parasites of the mosquitoes ochlerotatus triseriatus (say), aedes aegypti (linnaeus), and ae. albopictus (skuse), respectively. these 3 important vector ... | 2005 | 16539016 |
| molecular cloning and nucleotide sequence of cyp6bf1 from the diamondback moth, plutella xylostella. | a novel cdna clong encoding a cytochrome p450 was screened from the insecticide-susceptible strain of plutella xylostella (l.) (lepidoptera:yponomeutidae). the nucleotide sequence of the clone, designated cyp6bf1, was determined. this is the first full-length sequence of the cyp6 family from plutella xylostella (l.). the cdna is 1661bp in length and contains an open reading frame from base pairs 26 to 1570, encoding a protein of 514 amino acid residues. it is similar to the other insect p450s in ... | 2005 | 17119627 |
| dendritic cell precursors are permissive to dengue virus and human immunodeficiency virus infection. | cd14(+) interstitial cells reside beneath the epidermis of skin and mucosal tissue and may therefore play an important role in viral infections and the shaping of an antiviral immune response. however, in contrast to dendritic cells (dc) or blood monocytes, these antigen-presenting cells (apc) have not been well studied. we have previously described long-lived cd14(+) cells generated from cd34(+) hematopoietic progenitors, which may represent model cells for interstitial cd14(+) apc. here, we sh ... | 2005 | 15919883 |
| complement activation is required for induction of a protective antibody response against west nile virus infection. | infection with west nile virus (wnv) causes a severe infection of the central nervous system (cns) with higher levels of morbidity and mortality in the elderly and the immunocompromised. experiments with mice have begun to define how the innate and adaptive immune responses function to limit infection. here, we demonstrate that the complement system, a major component of innate immunity, controls wnv infection in vitro primarily in an antibody-dependent manner by neutralizing virus particles in ... | 2005 | 15919902 |
| single amino acid insertions at the junction of the sindbis virus e2 transmembrane domain and endodomain disrupt virus envelopment and alter infectivity. | the final steps in the envelopment of sindbis virus involve specific interactions of the e2 endodomain with the virus nucleocapsid. deleting e2 k at position 391 (e2 deltak391) resulted in the disruption of virus assembly in mammalian cells but not insect cells (host range mutant). this suggested unique interactions of the e2 deltak391 endodomain with the different biochemical environments of the mammalian and insect cell lipid bilayers. to further investigate the role of the amino acid residues ... | 2005 | 15919921 |
| generation of a novel wolbachia infection in aedes albopictus (asian tiger mosquito) via embryonic microinjection. | genetic strategies that reduce or block pathogen transmission by mosquitoes are being investigated as a means to augment current control measures. strategies of vector suppression and replacement are based upon intracellular wolbachia bacteria, which occur naturally in many insect populations. maternally inherited wolbachia have evolved diverse mechanisms to manipulate host insect reproduction and promote infection invasion. one mechanism is cytoplasmic incompatibility (ci) through which wolbach ... | 2005 | 15944085 |
| rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. | the development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (rt-lamp) assay targeting the 3' noncoding region for the rapid detection and differentiation of dengue virus serotypes are reported. the rt-lamp assay is very simple and rapid, wherein the amplification can be obtained in 30 min under isothermal conditions at 63 degrees c by employing a set of four serotype-specific primer mixtures ... | 2005 | 15956414 |
| bioecology and vectorial capacity of aedes albopictus (diptera: culicidae) in macao, china, in relation to dengue virus transmission. | until 2001, the chinese territory of macao had not registered any autochthonous dengue cases, despite the abundance of aedes albopictus (skuse), a known vector. this work describes a bioecological characterization of the local ae. albopictus adult population, with the purpose of estimating the receptivity of macao to dengue introduction. in the wet seasons of 1997 and 1998 and the dry season of 1998, ae. albopictus was the most abundant human-biting mosquito. daily biting rates of 314 mosquitoes ... | 2005 | 15962796 |
| characterization of plasma membrane-associated proteins from aedes albopictus mosquito (c6/36) cells that mediate west nile virus binding and infection. | this study isolated and characterized the west nile virus (wnv) putative receptor molecule(s) from aedes albopictus mosquito (c6/36) cells. the binding of wnv to c6/36 cells was saturated with 5000 particles per cell. the entry of wnv into c6/36 cells was strongly inhibited when pretreated with proteinase k and to a lesser extent with sodium periodate. however, pretreatment of c6/36 cells with phospholipases, glycosidases, heparinases and neurimidase had no effect on virus entry. by using virus ... | 2005 | 15992848 |
| castanospermine, a potent inhibitor of dengue virus infection in vitro and in vivo. | previous studies have suggested that alpha-glucosidase inhibitors such as castanospermine and deoxynojirimycin inhibit dengue virus type 1 infection by disrupting the folding of the structural proteins prm and e, a step crucial to viral secretion. we extend these studies by evaluating the inhibitory activity of castanospermine against a panel of clinically important flaviviruses including all four serotypes of dengue virus, yellow fever virus, and west nile virus. using in vitro assays we demons ... | 2005 | 15994763 |
| flow cytometry-based assay for titrating dengue virus. | plaque assays for titrating dengue virus (denv) are time-consuming and not suitable for strains that do not plaque. fluorescence-activated cell sorting (facs) has been used to detect denv-infected cells. here we describe a facs-based assay for titrating denv. we determined that at 24 h postinfection, the number of infected cells detected by facs represented the first round of infection and therefore could be used as a readout of the number of infectious particles in the inoculum. when the titers ... | 2005 | 16000446 |
| influence of bacillus thuringiensis var. israelensis on oviposition of aedes albopictus (skuse). | the effect of bacillus thuringiensis var israelensis (b.t.i.) on the oviposition behavior of aedes albopictus was evaluated in the field and laboratory in clemson, sc, u.s.a. in the field, water taken from containers in which mosquito larvae were reared (conditioned water) was placed in 16 containers. eight containers received 50 jld of b.t.i., and eight with water only were kept as controls. in the laboratory, field-collected females of ae. albopictus were placed in rearing chambers and provide ... | 2005 | 16007954 |
| evaluation of methods to assess transmission potential of venezuelan equine encephalitis virus by mosquitoes and estimation of mosquito saliva titers. | determining the dose of an arbovirus transmitted by a mosquito is important to design transmission and pathogenesis studies simulating natural infection. several different artificial infection and transmission methods used to assess vector competence and to estimate the dose injected during mosquito feeding have not been fully evaluated to determine whether they accurately reflect natural transmission. additionally, it is not known whether different mosquito vectors transmit similar amounts of a ... | 2005 | 16014828 |
| entomological investigation of a sylvatic yellow fever area in são paulo state, brazil. | following reports of two autochthonous cases of sylvatic yellow fever in the state of são paulo, brazil, in 2000, entomological surveys were conducted with the objective of verifying the occurrence of vector species in forest environments close to or associated with riparian areas located in the western and northwestern regions of the state. culicidae were captured in 39 sites distributed in four regions. haemagogus leucocelaenus and aedes albopictus were the most abundant species and were captu ... | 2005 | 16021266 |
| host-feeding patterns of suspected west nile virus mosquito vectors in delaware, 2001-2002. | paucity of data on host-feeding patterns and behavior of 43 mosquito species that are reported as suspected west nile virus (wn) vectors has limited full evaluation of their vectorial capacity. recent studies addressing this issue need additional confirmation and should also be expanded to include collections of species or subpopulations attracted to humans. we used 4 types of collection methods to collect mosquitoes, including omnidirectional fay-prince traps, centers for disease control-type l ... | 2005 | 16033122 |
| michelob_x is the missing inhibitor of apoptosis protein antagonist in mosquito genomes. | apoptosis is implicated in the life cycle of the malaria parasite in mosquitoes. the genome project for the primary malaria vector anopheles gambiae showed a significant expansion of the inhibitor of apoptosis protein (iap) and caspase gene families in comparison with drosophila. however, because of extensive sequence divergence, no orthologue was identified for the reaper/grim-like iap antagonist genes that have a pivotal role in cell death regulation in drosophila. using a customized searching ... | 2005 | 16041319 |
| mosquito salivary gland extracts induce ebv-infected nk cell oncogenesis via cd4 t cells in patients with hypersensitivity to mosquito bites. | severe hypersensitivity to mosquito bites (hmb) is characterized by intense local skin reactions and systemic symptoms such as high fever, lymphadenopathy, and hepatosplenomegaly. patients with hmb often have natural killer (nk) cell lymphocytosis associated with epstein-barr virus (ebv) infection. here we investigated whether mosquito bites have any influence on the oncogenesis of ebv-infected nk cells. we examined six hmb patients with ebv-infected nk cell lymphocytosis. we first demonstrated ... | 2005 | 16297196 |
| yunnan orbivirus, a new orbivirus species isolated from culex tritaeniorhynchus mosquitoes in china. | an orbivirus designated yunnan orbivirus (yuov) was isolated from culex tritaeniorhynchus mosquitoes collected in the yunnan province of china. electron microscopy showed particles with typical orbivirus morphology. the yuov genome was sequenced completely and compared with previously characterized orbivirus genomes. significant identity scores were detected between proteins encoded by the segments (seg-1 to seg-10) of yuov and those encoded by their homologues in insect-borne and tick-borne orb ... | 2005 | 16298988 |
| early events in the pathogenesis of eastern equine encephalitis virus in mice. | to elucidate the pathogenesis of eastern equine encephalitis (eee) virus infections, we used histopathology, immunohistochemistry, and in situ hybridization to track the spread and early cellular targets of viral infection in mice. young mice were inoculated with virulent eee virus in their right rear footpad and were followed in a time-course study for 4 days. virulent eee virus produced a biphasic illness characterized by an early self-limiting replication phase in peripheral tissues followed ... | 2005 | 15632009 |
| geographic distribution and breeding site preference of aedes albopictus and aedes aegypti (diptera: culicidae) in cameroon, central africa. | presence in cameroon of the recently introduced aedes (stegomyia) albopictus (skuse) in association with the indigenous aedes aegypti (l.) raises public heath concerns because it might alter the risk of arbovirus transmission. the breeding site and distribution of the two stegomyia species are updated and reported following entomological surveys carried out in 22 localities throughout cameroon, with a total of 1,353 containers with water visited. ae. aegypti was found in every location sampled, ... | 2005 | 16363155 |
| insecticide susceptibility of aedes aegypti and aedes albopictus across thailand. | aedes aegypti (l.) and aedes albopictus (skuse), two important vectors of dengue fever and dengue hemorrhagic fever, were collected from mae sot, nakhon sawan, nakhon ratchasima, surat thani, and phatthalung, thailand, from july 2003 to april 2004. the patterns of insecticide susceptibility to temephos, malathion, and permethrin of both ae. aegypti and ae. albopictus larvae were determined. ae. aegypti from all study sites were resistant to permethrin, they but were susceptible to malathion. res ... | 2005 | 16363166 |
| blood feeding patterns of aedes aegypti and aedes albopictus in thailand. | aedes aegypti (l.) and aedes albopictus (skuse) were collected with aspirators from mae sot, nakhon sawan, nakhon ratchasima, surat thani, and phatthalung study sites in thailand from july 2003 though april 2004. the sandwich-b enzyme-linked immunosorbent assay was used to analyze 1,021 blood-fed specimens. ae. aegypti almost exclusively fed on humans (99%, 658/664) in single host species, and 97% (86/88) of multiple-host bloodmeals included at least one human host. a low frequency of other host ... | 2005 | 16363170 |
| isolations of potosi virus from mosquitoes (diptera: culicidae) collected in connecticut. | potosi virus (potv) (bunyaviridae: orthobunyavirus) was first isolated from aedes albopictus (skuse) collected in potosi, mo, in 1989, and subsequent isolations were reported from illinois, michigan, ohio, and the carolinas. to determine whether the distribution of this virus extends into the northeastern united states, we analyzed arboviruses acquired from mosquitoes collected in connecticut from 1998 to 2004. in 2001, a bunyavirus was isolated from aedes vexans (meigen) that was different from ... | 2005 | 16363172 |
| [clinical and epidemiological aspects of japanese encephalitis]. | japanese encephalitis has been well controlled in japan. on the other hand, the japanese encephalitis virus (jev) transmission cycle still exists, as does the risk of exposure to jev infection. although culex tritaeniorhynchus has been considered the major mosquito vector in japan, recent surveys suggested culex pipiens complex and aedes albopictus as potential alternatives. similarly, swine have reduced the role in amplification of jev in paradomestic environments. another surveys suggested att ... | 2005 | 16363686 |
| eastern cottontail rabbits (sylvilagus floridanus) develop west nile virus viremias sufficient for infecting select mosquito species. | the potential of the eastern cottontail rabbit (ctr; sylvilagus floridanus) to contribute to an enzootic west nile virus (wnv) cycle was demonstrated by characterizing the wnv viremia profile of 15 ctrs and demonstrating that mosquitoes could become infected by feeding on these ctrs. eight ctrs were infected with a titer of 10(5.0) cell-infectious dose 50% endpoints (cid50s) of wnv (ny99-crow) by needle and seven ctrs by bite of one or more wnv-infected mosquitoes. there were no marked differenc ... | 2005 | 16417430 |
| repellent effect of plant essential oils against aedes albopictus. | six essential oils: asteraceae oil, rutaceae oil, mentha piperta oil, carvacryl oil, citronella oil, and eucalyptus oil were tested for evaluation of their repellent effects against aedes albopictus mosquitoes under laboratory conditions. only citronella oil and eucalyptus oil were tested with human beings. there was considerable protection for mice. carvacryl oil (7%) provided 100% protection for mice after 7 h. eucalyptus oil (15%) gave protection to humans for least 3 h; the protection time w ... | 2005 | 16599157 |
| occurrence of tire inhabiting mosquito larvae in different geographic regions of west virginia. | eleven species of mosquito larvae were collected in 568 collection visits to 209 abandoned tire pile sites in 33 west virginia counties from april through october of 2003. ochlerotatus j. japonicus supplanted the native oc. triseriatus as the most common tire-colonizing species in the state. in general, the exotic oc. j. japonicus was a better colonizer of tires than oc. triseriatus in peridomestic, sunlit conditions throughout the state, whereas both species were equally likely to colonize tire ... | 2005 | 16506562 |
| easily seen characters to identify the pupa of aedes albopictus in the united states. | pupal paddle characters of aedes albopictus that will quickly differentiate this species from other container-inhabiting species in the united states are described, illustrated, and compared with 2 other container species that have somewhat similar paddles, but with very different characters. additional noncontainer species that possess slightly similar characters are also discussed. citations for other published illustrations of the characters, pupal sexing methods, and published keys to pupae ... | 2005 | 16506571 |
| effectiveness of several locally available membranes used for artificial feeding of aedes albopictus skuse. | artificial feeding of mosquitoes with blood meal is an important technique in the studies of mosquito feeding. owing to the difficulty in obtaining suitable artificial membranes for mosquito feeding from other sources, several easily obtainable membranes in malaysia were tested for their suitability as a replacement. skin of chicken, fish, and salted sausage were obtained and tested against cattle skin membrane as a control. the results showed that cattle skin is still the most favorable membran ... | 2005 | 16880756 |
| field bioefficacy of deltamethrin residual spraying against dengue vectors. | field bioefficacy of residual-sprayed deltamethrin against aedes vectors was evaluated in an urban residential area in kuala lumpur. the trial area consisted of single storey wood-brick houses and a block of flat. the houses were treated with outdoor residual spraying while the flat was used as an untreated control. initial pre-survey using ovitrap surveillance indicated high aedes population in the area. deltamethrin wg was sprayed at a dosage of 25mg/m2 using a compression sprayer. the effecti ... | 2005 | 16883280 |
| weekly variation on susceptibility status of aedes mosquitoes against temephos in selangor, malaysia. | larvae of aedes aegypti and aedes albopictus obtained from 6 consecutive ovitrap surveillance (os) in taman samudera and kg. banjar were evaluated for their susceptibility to temephos. larval bioassays were carried out in accordance with who standard methods, with diagnostic dosage (0.012 mg/l) and operational dosage (1 mg/l) of temephos respectively. aedes aegypti and ae. albopictus obtained from six os in taman samudera showed resistance to diagnostic dosage of temephos with percentage mortali ... | 2005 | 16883288 |
| susceptibility of aedes aegypti and aedes albopictus to temephos in four study sites in kuala lumpur city center and selangor state, malaysia. | larvae obtained from taman samudera (gombak, selangor), kampung banjar (gombak, selangor), taman lembah maju (cheras, kuala lumpur) and kampung baru (city centre, kuala lumpur) were bioassayed with diagnostic dosage (0.012 mg/l) and operational dosage (1 mg/l) of temephos. all strains of aedes aegypti and aedes albopictus showed percentage mortality in the range of 16.00 to 59.05 and 6.4 to 59.50 respectively, after 24 hours. lt50 values for the 6 strains of ae. aegypti and ae. albopictus were b ... | 2005 | 16883289 |
| sensitivity of aedes albopictus c6/36 cells line for the detection and infectivity titration of dengue virus. | plaque assay procedure was carried out to determine the infectivity titration of dengue virus using aedes albopictus c6/36 mosquito cell line. cells were seeded in 6 wells plates and incubated until monolayer c6/36 cell lines were formed. each well was exposed to a different dilution of dengue infected culture fluid, followed by addition of first and second overlay medium. cells were observed under an inverted microscope and plaque staining was carried out. the results showed that countable plaq ... | 2005 | 16883290 |
| protein synthesized by dengue infected aedes aegypti and aedes albopictus. | the main objective of this study was to compare protein profiles of whole mosquitoes of malaysian aedes aegypti and aedes albopictus after infection with virus and to investigate whether dengue virus would induce protein secretion in ae. aegypti and ae. albopictus. using sds -page, it was shown that in uninfected ae. aegypti and ae. albopictus, the protein bands were within the range of 14 - 80 kda with most of the bands overlapping for the two species. comparison of the protein profile of infec ... | 2005 | 16883293 |
| a comparative study of adult mosquito trapping using dry ice and yeast generated carbon dioxide. | adult mosquito collections were conducted for 12 weeks in two residential areas in kuala lumpur. the cdc light traps were compared using dry ice and yeast as sources of carbon dioxide attractants for mosquitoes. the efficacy of the dry ice baited trap was significant over yeast generated co2 trap. the predominant species obtained were culex quinquefasciatus, stegomyia albopicta and armigeres subalbatus. | 2005 | 16883295 |
| comparative susceptibility of introduced forest-dwelling mosquitoes in hawai'i to avian malaria, plasmodium relictum. | to identify potential vectors of avian malaria in hawaiian native forests, the innate susceptibility of aedes albopictus, wyeomyia mitchellii, and culex quinquefasciatus from 3 geographical sites along an altitudinal gradient was evaluated using local isolates of plasmodium relictum. mosquitoes were dissected 5-8 and 9-13 days postinfective blood meal and microscopically examined for oocysts and salivary-gland sporozoites. sporogony was completed in all 3 species, but prevalence between species ... | 2005 | 17089752 |
| effects of wolbachia in the uzifly, exorista sorbillans, a parasitoid of the silkworm, bombyx mori. | the uzifly, exorista sorbillans (diptera: tachinidae), a parasitoid of the silkworm, bombyx mori l. (lepidoptera: bombycidae), harbours wolbachia (rickettsia) endosymbionts. administration of 0.05 mg/ml oxytetracycline to the adult uziflies removed wolbachia endosymbionts and resulted in different reproductive disorders, such as i) reduction in fecundity of uninfected females, ii) cytoplasmic incompatibility in crosses between infected males and uninfected females, iii) sterility in the crosses ... | 2005 | 17119612 |
| ecology of invasive mosquitoes: effects on resident species and on human health. | investigations of biological invasions focus on patterns and processes that are related to introduction, establishment, spread and impacts of introduced species. this review focuses on the ecological interactions operating during invasions by the most prominent group of insect vectors of disease, mosquitoes. first, we review characteristics of non-native mosquito species that have established viable populations, and those invasive species that have spread widely and had major impacts, testing wh ... | 2005 | 17637849 |
| aphid-symbiotic bacteria cultured in insect cell lines. | the cells and tissues of many aphids contain bacteria known as "secondary symbionts," which under specific environmental circumstances may be beneficial to the host insect. such symbiotic bacteria are traditionally described as intractable to cultivation in vitro. here we show that two types of aphid secondary symbionts, known informally as t type and u type, can be cultured and maintained in three insect cell lines. the identities of the cultured bacteria were confirmed by pcr with sequencing o ... | 2005 | 16085881 |
| epidemiology and transmission dynamics of west nile virus disease. | from 1937 until 1999, west nile virus (wnv) garnered scant medical attention as the cause of febrile illness and sporadic encephalitis in parts of africa, asia, and europe. after the surprising detection of wnv in new york city in 1999, the virus has spread dramatically westward across the united states, southward into central america and the caribbean, and northward into canada, resulting in the largest epidemics of neuroinvasive wnv disease ever reported. from 1999 to 2004, >7,000 neuroinvasiv ... | 2005 | 16102302 |
| west nile virus detection in urine. | we report west nile virus (wnv) rna in urine collected from a patient with encephalitis 8 days after symptom onset. viral rna was detected by reverse transcriptase-polymerase chain reaction (rt-pcr). sequence and phylogenetic analysis confirmed the pcr product to have > or = 99% similarity to the wnv strain ny 2000-crow3356. | 2005 | 16102323 |
| neuronal cxcl10 directs cd8+ t-cell recruitment and control of west nile virus encephalitis. | the activation and entry of antigen-specific cd8(+) t cells into the central nervous system is an essential step towards clearance of west nile virus (wnv) from infected neurons. the molecular signals responsible for the directed migration of virus-specific t cells and their cellular sources are presently unknown. here we demonstrate that in response to wnv infection, neurons secrete the chemokine cxcl10, which recruits effector t cells via the chemokine receptor cxcr3. neutralization or a genet ... | 2005 | 16103196 |
| application of an alternative aedes species (diptera: culicidae) surveillance method in botucatu city, sao paulo, brazil. | one of the main problems with dengue is the control of aedes aegypti, its major vector. in brazil, the current control program for ae. aegypti and aedes albopictus populations includes larval density surveys. an interesting alternative is the use of a distinct index, the premise condition index (pci). this tool relates conditions of property, such as houses and yards, and the degree of shade with the occurrence of aedes sp. oviposition, and is calculated as scores from 3 to 9. the lowest score i ... | 2005 | 16103596 |
| enhanced t-cell response to mosquito extracts by nk cells in hypersensitivity to mosquito bites associated with ebv infection and nk cell lymphocytosis. | hypersensitivity to mosquito bites is characterized by severe systemic as well as local symptoms, and associated with chronic active ebv infection and nk cell lymphocytosis. in this hen disease, we investigated the response of pbmc to msg extracts. pbmc were taken from three defined cases of hen disease, three borderline cases, five individuals with simple exaggerated reactions to mosquito bites without systemic symptoms (simple responders), and eight healthy donors. pbmc, or purified cd4+, cd8+ ... | 2005 | 16108834 |
| inducible nitric oxide synthase (inos) expression in monocytes during acute dengue fever in patients and during in vitro infection. | mononuclear phagocytes are considered to be main targets for dengue virus (denv) replication. these cells are activated after infection, producing proinflammatory mediators, including tumour-necrosis factor-alpha, which has also been detected in vivo. nitric oxide (no), usually produced by activated mononuclear phagocytes, has antimicrobial and antiviral activities. | 2005 | 16109165 |
| heteropentameric cholera toxin b subunit chimeric molecules genetically fused to a vaccine antigen induce systemic and mucosal immune responses: a potential new strategy to target recombinant vaccine antigens to mucosal immune systems. | noninvasive mucosal vaccines are attractive alternatives to parenteral vaccines. although the conjugation of vaccine antigens with the b subunit of cholera toxin (ctb) is one of the most promising strategies for vaccine delivery to mucosal immune systems, the molecule cannot tolerate large-protein fusion, as it severely impairs pentamerization and loses affinity for gm1-ganglioside. here we report a new strategy, in which steric hindrance between ctb-antigen fusion subunits is significantly redu ... | 2005 | 16113283 |
| [aedes albopictus in soil bromeliads in ilhabela, coastal area of southeastern brazil]. | to analyze the presence of aedes albopictus in soil bromeliads in ecologically distinct environments in terms of positivity, density and volume of water. | 2005 | 16113902 |
| recovery of cdnas encoding ribosomal proteins s9 and l26 from aedes albopictus mosquito cells and identification of their homologs in the malaria vector, anopheles gambiae. | we used pcr-based approaches to obtain the full-length cdna sequences encoding ribosomal protein (rp) s9 and l26 from a mosquito (aedes albopictus) c7-10 cell line. the deduced mosquito rps9 protein has a mass of 22,826 da and a pi of 11.41, while rpl26 had a mass of 17,442 da and a pi of 11.52. both cdnas initiated with the 5'-polypyrimidine motif characteristic of ribosomal protein transcripts. using the aedes protein and nucleic acid sequences, we identified rps9 and rpl26 as single copy gene ... | 2005 | 16116622 |
| asymmetrical competition and patterns of abundance of aedes albopictus and culex pipiens (diptera: culicidae). | we tested for competitive advantage among larvae of aedes albopictus (skuse) and culex pipiens l. in a laboratory experiment and determined the frequency and spatial and temporal patterns of co-occurrence in the field in east st. louis, il. in a laboratory competition experiment at multiple combined densities of ae. albopictus and cx. pipiens larvae, ae. albopictus survivorship and developmental times were significantly affected by conspecific densities but not by cx. pipiens densities. in contr ... | 2005 | 16119544 |
| strategically examining the full-genome of dengue virus type 3 in clinical isolates reveals its mutation spectra. | previous studies presented the quasispecies spectrum of the envelope region of dengue virus type 3 (denv-3) from either clinical specimens or field-caught mosquitoes. however, the extent of sequence variation among full genomic sequences of denv within infected individuals remains largely unknown. | 2005 | 16120221 |
| rapid cryopreservation of five mammalian and one mosquito cell line at -80 degrees c while attached to flasks in a serum free cryopreservative. | cell culturing, and the requisite storage of cell lines at ultra-low temperatures, is used in most laboratories studying or using eukaryotic proteomics, genomics, microarray, and rna technologies. in this study we have observed that a72(dog), crfk(cat), nb324k(human), mcf7(human), wi38(human), and c636(mosquito) cells were effectively cryopreserved at -80 degrees c while attached to the substratum of 25 cm2 tissue culture flasks. this was accomplished using a serum free crypreservative recently ... | 2005 | 16136221 |
| comparative sequence analysis of the eastern equine encephalitis virus pathogenic strains fl91-4679 and ga97 to other north american strains. | eastern equine encephalitis (eee) virus is a significant public health concern due to the high mortality rates observed in infected humans, equines and game birds. the eee genomic sequences available prior to this report are based on laboratory strains with unknown passage histories that may contain an array of cell culture adaptations. here we report the complete genomic sequences of two recently isolated eee pathogenic strains with low passage histories. fl91-4697 was isolated in florida from ... | 2005 | 16147892 |
| modulation of anopheles gambiae gene expression in response to o'nyong-nyong virus infection. | to determine if gene expression of an. gambiae is modulated in response to o'nyong-nyong virus (onnv) infection, we utilized cdna microarrays including about 20 000 cdnas. gene expression levels of onnv-infected female mosquitoes were compared to that of the uninfected control females harvested at 14 days postinfection. in response to onnv infection, expression levels of 18 genes were significantly modulated, being at least two-fold up- or down-regulated. quantitative real-time pcr analysis (qrt ... | 2005 | 16164603 |
| fitness consequences of anopheles gambiae population hybridization. | the use of transgenic mosquitoes with parasite inhibiting genes has been proposed as an integral strategy to control malaria transmission. however, release of exotic transgenic mosquitoes will bring in novel alleles along with parasite-inhibiting genes that may have unknown effects on native populations. thus it is necessary to study the effects and dynamics of fitness traits in native mosquito populations in response to the introduction of novel genes. this study was designed to evaluate the dy ... | 2005 | 16174295 |
| dog filariosis in the lazio region (central italy): first report on the presence of dirofilaria repens. | epidemiological investigations were carried out in the lazio region to assess the status of canine filariosis and to evaluate the actual risk for veterinary and medical public health. | 2005 | 16185352 |
| dengue virus inoculation to human skin explants: an effective approach to assess in situ the early infection and the effects on cutaneous dendritic cells. | although dengue virus (dv) enters through skin while mosquitoes feed, early contacts remain unexplored regarding the cutaneous viral fate and in situ immune responses. we addressed this by exposing healthy, non-cadaveric, freshly obtained human skin explants to a human dv2 isolate. we demonstrated negative-strand dv-rna and non-structural protein-1, both suggestive of viral replication in skin. although control, mock-infected and dv-infected explants showed less (mhc-cii(+)/cd1a(+)/langerin+) la ... | 2005 | 16191104 |
| effect of larval crowding on mating competitiveness of anopheles gambiae mosquitoes. | the success of sterile or transgenic anopheles for malaria control depends on their mating competitiveness within wild populations. current evidence suggests that transgenic mosquitoes have reduced fitness. one means of compensating for this fitness deficit would be to identify environmental conditions that increase their mating competitiveness, and incorporate them into laboratory rearing regimes. | 2005 | 16197541 |
| measuring the transmission dynamics of a sexually transmitted disease. | sexually transmitted diseases (stds) occur throughout the animal kingdom and are generally thought to affect host population dynamics and evolution very differently from other directly transmitted infectious diseases. in particular, stds are not thought to have threshold densities for persistence or to be able to regulate host population density independently; they may also have the potential to cause host extinction. however, these expectations follow from a theory that assumes that the rate of ... | 2005 | 16204382 |
| aedes (stegomyia) albopictus--a dengue threat for southern australia? | aedes albopictus, the so-called 'asian tiger mosquito,' which has invaded areas of the pacific, the americas, africa and europe, and been intercepted in various australian seaports in recent years, has now become established on a number of torres strait islands in northern queensland and threatens to invade mainland australia. as well as being a significant pest with day-biting tendencies, ae. albopictus is a vector of dengue viruses and is capable of transmitting a number of other arboviruses. ... | 2005 | 16220868 |
| s-phase-dependent enhancement of dengue virus 2 replication in mosquito cells, but not in human cells. | dengue virus (den) is the most prevalent cause of arthropod-borne viral illness in humans. we determined the influence of cellular growth state on den type 2 (den2) replication in mosquito and human cells, based on the hypothesis that manipulation of cellular growth state will facilitate identification of viral and cellular determinants of productive infection. comparison of density-arrested and cycling c6/36 aedes albopictus cells infected with a low-passage den2 isolate revealed that cycling c ... | 2005 | 16227245 |
| n-linked glycosylation of west nile virus envelope proteins influences particle assembly and infectivity. | west nile virus (wnv) encodes two envelope proteins, premembrane (prm) and envelope (e). while the prm protein of all wnv strains contains a single n-linked glycosylation site, not all strains contain an n-linked site in the e protein. the presence of n-linked glycosylation on flavivirus e proteins has been linked to virus production, ph sensitivity, and neuroinvasiveness. therefore, we examined the impact of prm and e glycosylation on wnv assembly and infectivity. similar to other flaviviruses, ... | 2005 | 16227249 |
| dengue virus type 3, brazil, 2002. | during the summer of 2002, rio de janeiro had a large epidemic of dengue fever; 288,245 cases were reported. a subset of 1,831 dengue hemorrhagic fever cases occurred. in this study, performed in the first half of 2002, samples from 1,559 patients with suspected cases of dengue infection were analyzed. from this total, 1,497 were obtained from patients with nonfatal cases, and 62 were obtained from patients with fatal cases. by the use of different methods, 831 (53.3%) cases, including 40 fatal ... | 2005 | 16229765 |
| west nile virus-infected mosquitoes, louisiana, 2002. | human cases of west nile virus (wnv) disease appeared in st. tammany and tangipahoa parishes in southeastern louisiana in june 2002. cases peaked during july, then rapidly declined. we conducted mosquito collections from august 3 to august 15 at residences of patients with confirmed and suspected wnv disease to estimate species composition, relative abundance, and wnv infection rates. a total of 31,215 mosquitoes representing 25 species were collected by using primarily gravid traps and co2-bait ... | 2005 | 16229769 |
| laboratory and field evaluation of the insect growth regulator pyriproxyfen (sumilarv 0.5g) against dengue vectors. | the insect growth regulator pyriproxyfen was tested against aedes aegypti at 0.01 and 0.02 mg of active ingredient (ai) per liter of water in 60-liter earthern jars. both concentrations provided 100% control for 4 months. in additional experiments where 10 liters of water were replaced fortnightly, 100% control was still obtained over 4 months with 0.02 mg ai/liter and greater than 93-100% control was obtained over 4 months with 0.01 mg ai/liter. in less-controlled field-trial conditions, pyripr ... | 2005 | 16252520 |
| identification of aedes albopictus in urban nicaragua. | larvae of aedes albopictus, a mosquito known for transmitting dengue virus, were identified in the city of león, nicaragua, in 2003. mosquito larvae were collected from a total of 2,225 residences in the 2 largest cities in nicaragua during the period from june to september of 2003, and larval ae. albopictus were identified in 4 homes in león. this represents the 1st detection of ae. albopictus in a major nicaraguan urban center, and increased control efforts appear to have eliminated the mosqui ... | 2005 | 16252527 |
| effect of alternating passage on adaptation of sindbis virus to vertebrate and invertebrate cells. | mosquito-borne alphaviruses, which replicate alternately and obligately in mosquitoes and vertebrates, appear to experience lower rates of evolution than do many rna viruses that replicate solely in vertebrates. this genetic stability is hypothesized to result from the alternating host cycle, which constrains evolution by imposing compromise fitness solutions in each host. to test this hypothesis, sindbis virus was passaged serially, either in one cell type to eliminate host alteration or altern ... | 2005 | 16254360 |
| efficient procedure for purification of obligate intracellular wolbachia pipientis and representative amplification of its genome by multiple-displacement amplification. | bacteria belonging to the genus wolbachia are obligatory microendocytobionts that infect a variety of arthropods and a majority of filarial nematode species, where they induce reproductive alterations or establish a mutualistic symbiosis. although two whole genome sequences of wolbachia pipientis, for strain wmel from drosophila melanogaster and strain wbm from brugia malayi, have been fully completed and six other genome sequencing projects are ongoing (http://www.genomesonline.org/index.cgi?wa ... | 2005 | 16269725 |
| factors influencing in vitro infectivity and growth of rickettsia peacockii (rickettsiales: rickettsiaceae), an endosymbiont of the rocky mountain wood tick, dermacentor andersoni (acari, ixodidae). | rickettsia peacockii, a spotted fever group rickettsia, is a transovarially transmitted endosymbiont of rocky mountain wood ticks, dermacentor andersoni. this rickettsia, formerly known as the east side agent and restricted to female ticks, was detected in a chronically infected embryonic cell line, dae100, from d. andersoni. we examined infectivity, ability to induce cytopathic effect (cpe) and host cell specificity of r. peacockii using cultured arthropod and mammalian cells. aposymbiotic dae1 ... | 2005 | 16288906 |
| a cell-free mrna stability assay reveals conservation of the enzymes and mechanisms of mrna decay between mosquito and mammalian cell lines. | the rate of mrna turnover is an important determinant of levels of gene expression. although this process has been studied extensively in mammalian cells and yeast, relatively little is known about the mrna decay pathways in insects. our analysis found that the vast majority of components of the mrna decay machinery are conserved between humans and mosquitoes. moreover, the half-lives of aedes albopictus mrnas are within a similar range to those of mammalian mrnas. in order to investigate mechan ... | 2005 | 16291088 |
| [distribution of aedes albopictus (skuse) in nuevo león, méxico, 2001-2004]. | to determine the distribution of aedes albopictus (skuse) in nuevo leon between 2001 and 2004. | 2005 | 15889642 |
| dengue fever, hawaii, 2001-2002. | autochthonous dengue infections were last reported in hawaii in 1944. in september 2001, the hawaii department of health was notified of an unusual febrile illness in a resident with no travel history; dengue fever was confirmed. during the investigation, 1,644 persons with locally acquired denguelike illness were evaluated, and 122 (7%) laboratory-positive dengue infections were identified; dengue virus serotype 1 was isolated from 15 patients. no cases of dengue hemorrhagic fever or shock synd ... | 2005 | 15890132 |
| dengue type 3 virus, saint martin, 2003-2004. | we describe the spread of a dengue virus during an outbreak in saint martin island (french west indies) during winter 2003-2004. dengue type 3 viruses were isolated from 6 patients exhibiting clinical symptoms. this serotype had not been detected on the island during the preceding 3 years. genome sequence determinations and analyses showed a common origin with dengue type 3 viruses isolated in martinique 2 years earlier. | 2005 | 15890134 |
| recombinant canarypox vectored west nile virus (wnv) vaccine protects dogs and cats against a mosquito wnv challenge. | the safety and efficacy of a canarypox vector expressing prm and e genes of west nile virus (wnv) (alvac-wnv) was evaluated in dogs and cats. one group of 17 dogs (vaccinated with 10(5.6) tcid(50)) and two groups of cats (groups 1 [n=14] vaccinated with 10(7.5) tcid(50) and 2 [n=8] 10(5.6) tcid(50)) were vaccinated twice at 28-day intervals. fifteen dogs and eleven cats served as negative controls. the cats and dogs were challenged 120 and 135 days after the second immunization, respectively via ... | 2005 | 15893618 |
| [anthropophilic activity of aedes aegypti and of aedes albopictus in area under control and surveillance]. | to describe the hematophagous activity of ae. aegypti and ae. albopictus in an area under control and surveillance. | 2005 | 15895139 |