Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs. | antigens were identified from the third-stage larvae (l3) and lung-stage larvae of ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated ascaris suum larval infections. forty-seven and 13 spots were recognized as antigens from the l3 and lung-stage larvae, respectively. their apparent molecular weight ranged from 20 to 101 kda and their isoelectric point from 3.6 to 8.0. the present study provides a framework for further molecular ... | 2001 | 11459018 |
| involvement of neuropeptides in the allergic nasal obstruction in guinea pigs. | the purposes of the present study were i) to determine whether neuropeptides induce the nasal obstruction in guinea pigs, and ii) to examine the possible involvement of neuropeptides in allergic nasal obstruction. the decrease in nasal cavity volume was determined by acoustic rhinometry as an index of nasal obstruction. in non-sensitized guinea pigs, substance p (sp), neurokinin a (nka) and calcitonin gene-related peptide (cgrp) caused the nasal obstruction 10 to 30 min after their intranasal ap ... | 2001 | 11459122 |
| the influence of stocking rate on transmission of helminth parasites in pigs on permanent pasture during two consecutive summers. | this study was made to elucidate the transmission of nematode infections in outdoor pigs at different stocking rates during two consecutive seasons. five pigs (group 1a) inoculated with low doses of oesophagostomum dentatum, ascaris suum, and trichuris suis and five helminth-naïve pigs (group 1b) were turned out together in june 1996 on each of four pastures at stocking rates of 100, 240 (two pastures) and 576m(2) per pig, respectively. the pigs were slaughtered in early october, and pasture inf ... | 2001 | 11470180 |
| production and characterization of a monoclonal antibody against an ascaris suum allergenic component. | ascaris suum allergenic components (piii) separated by gel filtration chromatography of an adult worm extract were used to immunize balb/c mice. popliteal lymph node cells taken from the immunized animals were fused with sp2/o myeloma cells using polyethylene glycol (mw 1450) as fusogen. the hybridomas were cultured in hat-containing medium and cloned at limiting dilutions. supernatants from the growing hybrids were screened by elisa using plates coated with piii or the a. suum crude extract. th ... | 2001 | 11471042 |
| possible presence of common tyvelose-containing glycans in trichinella l1 larvae and embryonated eggs of several nematodes. | a monoclonal antibody (mab us4) recognising an epitope containing tyvelose within the t. spiralis l-1 muscle larvae (tsl-1) antigens was tested in western-blot against various antigenic preparations from different stages of the following nematodes: t. spiralis (l1, adult), t. muris (egg, l1, l3, adult), ascaris suum (egg, adult), toxocara canis (egg, adult), anisakis simplex (l3) and haemochus contortus (egg). positive reaction was present in antigen preparations from l1 larvae of t. spiralis an ... | 2001 | 11484333 |
| preclinical efficacy and safety of mepolizumab (sb-240563), a humanized monoclonal antibody to il-5, in cynomolgus monkeys. | allergic respiratory diseases are characterized by large numbers of eosinophils and their reactive products in airways and blood; these are believed to be involved in progressive airway damage and remodeling. il-5 is the principal cytokine for eosinophil maturation, differentiation, and survival. mepolizumab (sb-240563), a humanized monoclonal antibody (mab) specific for human il-5, is currently in clinical trials for treatment of asthma. | 2001 | 11496242 |
| mutagenic and chemical modification of the aba-1 allergen of the nematode ascaris: consequences for structure and lipid binding properties. | the polyprotein allergens/antigens of nematodes (npas) are the only lipid binding proteins known to be produced as polyproteins. cleavage of the large polyprotein precursors at regularly spaced proteinase cleavage sites produces 10 or 11 individual protein units of approximately 15 kda. the sequences of these units are highly diverse within and between species, but there are five absolutely or strongly conserved amino acid positions (trp15, gln20, leu42, cys64, and cys120). we have tested the ro ... | 2001 | 11502186 |
| optimization of the agar-gel method for isolation of migrating ascaris suum larvae from the liver and lungs of pigs. | experiments on use of an agar-gel method for recovery of migrating ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% nacl at 38 degrees c. the results showed that within 3 h more than 88% of the recoverable larvae migrated out of the liver agar-gel and more than 83% of t ... | 2001 | 11503373 |
| isolation and preliminary biological assessment of aadgaplirfamide and svpgvlrfamide from caenorhabditis elegans. | to date, 9 fmrfamide-related peptides (farps) have been structurally characterised from caenorhabditis elegans. radioimmunometrical screening of an ethanolic extract of c. elegans revealed the presence of two additional farps that were purified by reverse-phase hplc and subjected to edman degradation analysis and gas-phase sequencing. unequivocal primary structures for the two farps were determined as ala-ala-asp-gly-ala-pro-leu-ile-arg-phe-nh(2) and ser-val-pro-gly-val-leu-arg-phe-nh(2). using ... | 2001 | 11527423 |
| [relationship between the prevalence of hepatic milk spots in pig and the egg density of ascaris suum in kitakyushu municipal meat inspection and control center]. | the hepatic milk spots of pigs are chronic interstitial hepatitis, and are caused by the migration of pig ascarid worm, ascaris suum, into the liver. a large number of livers are condemned in meat inspection centers as defective food once they are found to have developed milk spots, and thus the economic loss is great. eggs of ascaris suum from 120 caecum feces of slaughtered pigs were detected by the nylon mesh filtration (makiya) technique and they were related to the condemnation data of the ... | 2001 | 11570049 |
| release of hyaluronidase during in vitro development of ascaris suum from the third to fourth larval stage. | an enzyme that degraded glycosaminoglycan hyaluronic acid was released during in vitro development of ascaris suum l3 to l4. the enzyme did not hydrolyze glycosaminoglycan chondroitin sulfate a. one molecular form of hyaluronidase was detected, with a molecular weight estimated at 47.8 +/- 8.6 kda by sucrose density gradient centrifugation and at 55.0 +/- 1.3 kda by substrate sds-page zymography. activity of the enzyme was optimal between ph 5.0 and 6.0, and was present at neutral ph. hyaluronid ... | 2001 | 11570551 |
| purification of anisakis simplex antigen by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the diagnosis of human anisakidosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg-specific antibodies were isolated by means of protein a-sepharose cl-4b bead columns. igg anti-anisak ... | 2001 | 11570559 |
| a model for an ascaris muscle cell. | muscle cells in the nematode ascaris suum undergo bouts of oscillation in the behaving worm, these being correlated with progressing waves of contraction along the body of the worm. the bouts have three time scales: a rough period of 7-20 s, 10-20 short bursts of spikes within a bout, and three to eight spikes per burst. this paper has two aims: showing that there is consistency between measurements of individual currents and measurements on whole muscle cells, and creating a building block to e ... | 2001 | 11571482 |
| chickens and pigs as transport hosts for ascaris, trichuris and oesophagostomum eggs. | ten chickens and 2 pigs were fed non-embryonated eggs of ascaris suum, trichuris suis and oesophagostomum dentatum. each chicken was fed approximately 15,000 eggs of each parasite species while approximately 300,000 eggs were given to each of the pigs. after passage in chickens 8.3% of o. dentatum eggs were recovered in faeces compared to 61.1% and 38.4%, 49.1% and 30.3%, 41.6% of a. suum and t. suis eggs, respectively. after passage in pigs the percentages were respectively. after embryonation ... | 2001 | 11578097 |
| ecological influences on transmission rates of ascaris suum to pigs on pastures. | a study was conducted to determine the distribution and transmission rate of ascaris suum eggs and oesophagostomum dentatum larvae in a pasture/pig house facility, which during the preceding summer was contaminated with helminth eggs by infected pigs. in may, four groups of 10 helminth naïve tracer pigs were exposed to fenced sections of the facility for 7 days and necropsied for parasite recovery 9-10 days later (trial 1). the highest rate of a. suum transmission (201 eggs per day) occurred in ... | 2001 | 11587843 |
| natural antibodies to nematode biotinyl-enzymes in human sera. | biotinyl-enzymes are conservative molecules present in helminths, as well as in other animals, bacteria and plants. they have recently been found to be antigenic in mice, and a potential source of cross-reactivity among helminths. this study investigated the presence in human sera of antibodies reactive with biotinyl-enzymes from the nematodes anisakis simplex, toxocara canis and ascaris suum. biotinyl-enzymes from all these nematodes were recognized by igg1 antibodies in sera from healthy subje ... | 2001 | 11599787 |
| the effect of a bradykinin b2 receptor antagonist, npc-567, on allergen-induced airway responses in a porcine model. | in order to assess the effect of selective blocking of the bradykinin (bk) b2 receptor in allergic airway reactions, the bk b2 receptor antagonist npc-567 was administered to sensitized pigs before allergen challenge. | 2001 | 11603850 |
| cuticular collagen synthesis by ascaris suum during development from the third to fourth larval stage: identification of a potential chemotherapeutic agent with a novel mechanism of action. | the dominant proteins released by ascaris suum during development in vitro from the l3 to l4 stage were identified as collagenous cuticular proteins by sequence analysis and susceptibility to digestion by collagenase. under reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), the collagen proteins separated into 3 groups with molecular weights estimated at 32 kda, 54-60 kda, and 71-91 kda. the 32-kda protein represents monomeric collagen; the 54-60- and 71-91-kda compon ... | 2001 | 11695381 |
| selective protective effects of nitric oxide inhalation on allergen-induced acute airway reactions in the pig. | nitric oxide (no) is thought to be an important mediator of inflammatory processes during allergic reactions in the respiratory tract. | 2001 | 11696056 |
| intranasal immunization with recombinant ascaris suum 14-kilodalton antigen coupled with cholera toxin b subunit induces protective immunity to a. suum infection in mice. | animals can be rendered immune to ascaris parasites by immunization with infectious-stage larvae. the specific parasite gene products that mediate protective responses in ascariasis are unknown. we have identified a cdna encoding ascaris suum 14-kda antigen (as14) and evaluated the vaccinal effect of the escherichia coli-expressed recombinant protein (ras14). genbank analysis showed that as14 has low similarity at the amino acid level to a caenorhabditis elegans gene product and to antigens of t ... | 2001 | 11705899 |
| marginal vitamin a deficiency in pigs experimentally infected with trichuris suis: a model for vitamin a inadequacy in children. | the development of an experimental model for marginal vitamin a deficiency in humans is of major interest, enabling the elucidation of possible interactions with helminth infections. we established a useful experimental model for human vitamin a deficiency in young pigs; deficiency was induced through a depletion method encompassing both sow and offspring. we report on a 2 x 2 study in which 18-week-old vitamin a deficient pigs and vitamin a sufficient littermates were infected with both of the ... | 2001 | 11706673 |
| inactivation of viable ascaris eggs by reagents during enumeration. | various reagents commonly used to enumerate viable helminth eggs from wastewater and sludge were evaluated for their potential to inactivate ascaris eggs under typical laboratory conditions. two methods were used to enumerate indigenous ascaris eggs from sludge samples. all steps in the methods were the same except that in method i a phase extraction step with acid-alcohol (35% ethanol in 0.1 n h(2)so(4)) and diethyl ether was used whereas in method ii the extraction step was avoided by pouring ... | 2001 | 11722892 |
| ex vivo diffusion of albendazole and its sulfoxide metabolite into ascaris suum and fasciola hepatica. | the current experiments compare the pattern of ex vivo uptake (diffusion) of albendazole (abz) and albendazole sulfoxide (abzso) by ascaris suum and fasciola hepatica. specimens of a. suum and f. hepatica were collected from untreated animals (pigs and sheep, respectively) and incubated with either abz or abzso for different time periods (5-180 min). after incubation. the parasite material was analysed by hplc to quantify the amount of abz and/or abzso. the parent drug and its active abzso metab ... | 2001 | 11728018 |
| [two cases of visceral larva migrans due to ascaris suum showing a migratory nodular shadow]. | the number of cases of visceral larva migrans caused by the pig ascarid, ascaris suum has recently been increasing. we have encountered two cases of visceral larva migrans due to a. suum with a nodular shadow on the chest radiograph and eosinophilia in the peripheral blood. patient 1 was a 26-year-old man who had been admitted to our hospital for an elective minor operation. his chest radiology and chest computed tomography revealed a nodule in the left lung field. peripheral blood eosinophil co ... | 2001 | 11729695 |
| ascaris and ascariasis. | in recent years much new information has been obtained about the epidemiology, population biology and public health significance of infections of ascaris lumbricoides in humans. results from experimental infections of a. suum in pigs have helped to elucidate the observations made in the community on human ascariasis. the main purpose of the review is to see how new information may contribute to further acceptance of ascariasis as a serious contributor to ill-health and so to the design and imple ... | 2001 | 11013758 |
| airway resistance and tissue elastance from input or transfer impedance in bronchoconstricted monkeys. | ascaris suum (as) challenge in nonhuman primates is used as an animal model of human asthma. the primary goal of this study was to determine whether the airways and respiratory tissues in monkeys that are bronchoconstricted by as inhalation behave similarly to those in asthmatic humans. airway resistance (raw) and tissue elastance (eti) were estimated from respiratory system input (zin) or transfer (ztr) impedance. zin (0.4-20 hz) and ztr (2-128 hz) were measured in anesthetized cynomolgus monke ... | 2001 | 11160055 |
| structure-activity relationships of 18 endogenous neuropeptides on the motor nervous system of the nematode ascaris suum. | neuropeptides play an important role in all nervous systems and structure-activity studies of related peptides is one approach to understanding this role. this study of the motor nervous system of the parasitic nematode ascaris suum describes the physiological effects of a family of 18 endogenous ascaris fmrfamide-like peptides (af peptides) on the membrane potential and input resistance of the dorsal excitatory type 2 (de2) and dorsal inhibitory (di) motor neurons. these motor neurons are part ... | 2001 | 11179593 |
| an anthelmintic compound, nafuredin, shows selective inhibition of complex i in helminth mitochondria. | infections with parasitic helminths are important causes of morbidity and mortality worldwide. new drugs that are parasite specific and minimally toxic to the host are needed to counter these infections effectively. here we report the finding of a previously unidentified compound, nafuredin, from aspergillus niger. nafuredin inhibits nadh-fumarate reductase (complexes i + ii) activity, a unique anaerobic electron transport system in helminth mitochondria, at nm order. it competes for the quinone ... | 2001 | 11120889 |
| extracts of ascaris suum egg and adult worm share similar immunosuppressive properties. | adult ascaris suum body extract (asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of dba/2 mice to ovalbumin (oa) and preferentially stimulates a th2 response to its own components, which is responsible for the suppression of the oa-specific th1 response. here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (e-asc) in these immunological events. extracts from either sex (1 mg/animal) or ... | 2002 | 11743619 |
| controlling ligand binding in myoglobin by mutagenesis. | a quadruple mutant of sperm whale myoglobin was constructed to mimic the structure found in ascaris suum hemoglobin. the replacements include his(e7)-->gln, leu(b10)-->tyr, thr(e10)--> arg, and ile(g8)-->phe. single, double, and triple mutants were characterized to dissect out the effects of the individual substitutions. the crystal structures of the deoxy and oxy forms of the quadruple mutant were determined and compared with that of native ascaris hemoglobin. tyr(b10) myoglobin displays low o( ... | 2002 | 11744723 |
| molecular characterization of a glyceraldehyde-3-phosphate dehydrogenase from the swine roundworm ascaris suum. | 2002 | 11755196 | |
| new telomere formation during the process of chromatin diminution in ascaris suum. | chromatin diminution in the parasitic nematode ascaris suum represents an interesting case of developmentally programmed dna rearrangement in higher eukaryotes. at the molecular level, it is a rather complex event including chromosome breakage, new telomere formation and dna degradation. analysis of a cloned somatic telomere (ptel1) revealed that it has been newly created during the process of chromatin diminution by the addition of telomeric repeats (ttaggc)n to a chromosomal breakage site (mül ... | 2002 | 11902675 |
| the minimal trna: unique structure of ascaris suum mitochondrial trna(ser)(ucu) having a short t arm and lacking the entire d arm. | the tertiary structure of ascaris suum mitochondrial trna(ser)(ucu) was examined by nuclear magnetic resonance analysis using its transcript, since trna(ser)(ucu), lacking the d arm and possessing a truncated t arm, is the shortest of all the known trnas. most basepairs in the proposed secondary structure of trna(ser)(ucu) were shown to exist, but the connector region comprising the truncated d loop and the extra loop was flexible. this flexibility, would enable adjustment of the mutual distance ... | 2002 | 11904178 |
| [zafirlukast (accolate): a review of its pharmacological and clinical profile]. | today, bronchial asthma is considered as a chronic inflammatory disease of the airway. it has been revealed that various chemical mediators are involved in the onset of bronchial asthma. among them, particularly, the peptide leukotrienes, ltc4, ltd4 and lte4, have been known to play a pathophysiological important role in asthma. zafirlukast binds to the cyslt1 receptors competitively with these peptide leukotrienes and inhibited peptide leukotriene-induced constriction of isolated guinea pig tra ... | 2002 | 11979731 |
| enantioselective binding of albendazole sulphoxide to cytosolic proteins from helminth parasites. | the pharmacological effect of the active albendazole metabolite, albendazole sulphoxide (abzso), depends on its sustained presence at the site of parasite location and its binding to helmith beta-tubulin. abzso is found in the plasma and tissues of albendazole-treated animals in two enantiomeric forms: (+)abzso and (-)abzso. knowledge of enantioselectivity in drug action is necessary, since any difference in target proteins affinity between enantiomers may have implications on the pharmacologica ... | 2002 | 11980387 |
| evaluation of cell-surface ige receptors on the canine mastocytoma cell line c2 maintained in continuous culture. | to assess expression and function of cell-surface ige receptors on the canine mastocytoma cell line c2 maintained in continuous culture. | 2002 | 12013481 |
| evaluation by elisa of anisakis simplex larval antigen purified by affinity chromatography. | in order to improve the specificity and sensitivity of the techniques for the human anisakidosis diagnosis, a method of affinity chromatography for the purification of species-specific antigens from anisakis simplex third-stage larvae (l3) has been developed. new zealand rabbits were immunized with a. simplex or ascaris suum antigens or inoculated with toxocara canis embryonated eggs. the igg specific antibodies were isolated by means of protein a-sepharose cl-4b beads columns. igg anti-a. simpl ... | 2002 | 12016451 |
| how helminth lipid-binding proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the aba-1 polyprotein allergen and ov-far-1 proteins of nematodes and sj-fabpc of schistosomes. | three different classes of small lipid-binding protein (lbp) are found in helminth parasites. although of similar size, the aba-1a1 (also designated as-npa-a1) and ov-far-1 (formerly known as ov20) proteins of nematodes are mainly alpha-helical and have no known structural counterparts in mammals, whereas sj-fabpc of schistosomes is predicted to form a beta-barrel structure similar to the mammalian family of intracellular fatty acid binding proteins. the parasites that produce these proteins are ... | 2002 | 12022874 |
| crystal structure of the malic enzyme from ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 a resolution. | the structure of the ascaris suum mitochondrial nad-malic enzyme in binary complex with nad has been solved to a resolution of 2.3 a by x-ray crystallography. the structure resembles that of the human mitochondrial enzyme determined in complex with nad [xu, y., bhargava, g., wu, h., loeber, g., and tong, l. (1999) structure 7, 877-889]. the enzyme is a tetramer comprised of subunits possessing four domains organized in an "open" structure typical of the nad-bound form. the subunit organization, ... | 2002 | 12033925 |
| synthetic studies on glycosphingolipids from protostomia phyla: synthesis of amphoteric glycolipid analogues from the porcine nematode, ascaris suum. | a novel amphoteric glycosphingolipid, cholinephosphoryl-(-->6)-beta-d-glcpnac-(1-->3)-beta-d-manp-(1-->4)-beta-d-glcp-(1-->)-cer, isolated from the porcine parasitic nematode, ascaris suum, may be expected to be involved in host-parasite interactions. this glycosphingolipid analogue containing octyl residue in place of ceramide was synthesized as follows: the key reaction of this synthetic procedure is the formation of a intramolecular aglycon delivery (iad) approach for beta-selective mannosyla ... | 2002 | 12036012 |
| the tryptase inhibitor apc-366 reduces the acute airway response to allergen in pigs sensitized to ascaris suum. | tryptase is a mast cell serine protease that is released during mast cell degranulation. it has been implicated as an important enzyme in the pathophysiology of asthma, but its role in this disease is not fully elucidated. | 2002 | 12047446 |
| impact of protein energy malnutrition on trichuris suis infection in pigs concomitantly infected with ascaris suum. | the objective of this experiment was to investigate a possible interaction between protein and energy malnutrition (pem) and intestinal nematode infections. we report on a 3 x 2 factorial study in which pigs were fed either a low protein energy (lpe), low protein (lp) or a normal protein energy (npe) diet, and 6 weeks later inoculated with trichuris suis (4000 infective eggs). secondarily, in order to obtain a polyparasitic status, pigs were concomitantly inoculated with ascaris suum (600 infect ... | 2002 | 12049419 |
| microarray profile of differentially expressed genes in a monkey model of allergic asthma. | inhalation of ascaris suum antigen by allergic monkeys causes an immediate bronchoconstriction and delayed allergic reaction, including a pulmonary inflammatory infiltrate. to identify genes involved in this process, the gene-expression pattern of allergic monkey lungs was profiled by microarrays. monkeys were challenged by inhalation of a. suum antigen or given interleukin-4 (il-4) treatment; lung tissue was collected at 4, 18 or 24 h after antigen challenge or 24 h after il-4. each challenged ... | 2002 | 12049661 |
| 2.6 a resolution crystal structure of helices of the motile major sperm protein (msp) of caenorhabditis elegans. | the amoeboid locomotion of nematode sperm is mediated by the assembly dynamics of the major sperm protein (msp). msp forms fibrous networks based on a hierarchy of macromolecular assemblies: helical subfilaments are built from msp dimers; filaments are formed from two subfilaments coiling round one another; and filaments themselves supercoil to produce bundles. to provide a structural context for understanding the role of these macromolecular assemblies in cell locomotion, we have determined the ... | 2002 | 12051923 |
| alternative migration routes of ascaris suum in the pig. | experiments were conducted to investigate possible alternative routes of extraintestinal migration of ascaris suum larvae in the pig. pigs were infected with a. suum via injection of newly hatched larvae into cecal veins (i.v.), into cecal lymph nodes (ln), or intraperitoneally (i.p.), and control animals were inoculated orally with infective eggs (p.o.). two pigs per inoculation route were necropsied on days 1, 4, and 13 postinoculation. the numbers of liver lesions and the percentage of larvae ... | 2002 | 12053960 |
| helminth infections in danish organic swine herds. | in nine organic swine herds, faecal excretion and pasture contamination by parasite eggs/larvae were studied in a period from march to october 1999. it was shown that the organic pigs were infected with ascaris suum (28% of weaners, 33% of fatteners, 4% of sows), trichuris suis (4% of weaners, 13% of fatteners, <1% of sows) and oesophagostomum spp. (5% of weaners, 14% of fatteners, 20% of sows) whereas no infections with hyostrongylus rubidus, metastrongylus spp. or strongyloides ransomi were de ... | 2002 | 12062513 |
| pharmacology of n-(3,5-dichloro-1-oxido-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 351591), a novel, orally active phosphodiesterase 4 inhibitor. | n-(3,5-dichloro-1-oxido-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 351591) has been identified as a potent (ic(50) = 58 nm) and highly selective type 4 phosphodiesterase (pde4) inhibitor with oral bioactivity in several animal models of lung inflammation. n-(3,5-dichloro-4-pyridinyl)-8-methoxy-2-(trifluoromethyl)-5-quinoline carboxamide (sch 365351), the only significant in vivo metabolite, is also a potent and highly selective pde4 inhibitor (ic(50) = 20 nm). both s ... | 2002 | 12065709 |
| a cathepsin l protease essential for caenorhabditis elegans embryogenesis is functionally conserved in parasitic nematodes. | proteolytic enzymes are involved in processes important to development and survival of many organisms. parasite proteases are considered potential targets of parasite control yet, for most, their precise physiological functions are unknown. validation of potential targets requires analysis of function. we have recently identified a cathepsin l (cpl) cysteine protease, ce-cpl-1, which is essential for embryonic development of the free-living nematode caenorhabditis elegans. we now show that cpl g ... | 2002 | 12076767 |
| ascarid nematodes in domestic and wild terrestrial mammals. | the biology of the ascarid nematodes has been discussed in the context of their important economic role in farm animals, pet animals and zoo animals with special attention to carnivores and primates. in farm animals, infection with the most common roundworm of horses (parascaris equorum) and swine (ascaris suum) depend on many factors such as environmental conditions (larval development in the egg and egg survival), age of the host, breed, husbandry system, hygiene and treatment schedule. the mo ... | 2002 | 12512563 |
| pathogenic intestinal parasites and bacterial agents in solid wastes. | to determine the profile of potentially pathogenic enteric parasites and bacterial agents in municipal refuse dumps in ibadan, nigeria. | 2002 | 12630495 |
| [observation on the survival and invasibility of ascaris suum egg preserved at low temperature for 5 years]. | 2002 | 12567985 | |
| eosinophilic inflammation and airway hyper-responsiveness are profoundly inhibited by a helminth (ascaris suum) extract in a murine model of asthma. | the increase of atopic disorders in developed countries has been associated with the decline of infectious diseases, including helminthic infections. we have already demonstrated that adult worm extracts from ascaris suum (asc) suppress the ige antibody production against unrelated antigens. | 2002 | 12569989 |
| the time-course of the response to the fmrfamide-related peptide pf4 in ascaris suum muscle cells indicates direct gating of a chloride ion-channel. | we investigated the effects of pf4 on ascaris suum somatic muscle cells using a 2 electrode current-clamp technique. pf4 is a farp (fmrfamide-related peptide), originally isolated from the free-living nematode panagrellus redivivus. pf4 caused hyperpolarization and an increase in chloride ion conductance when it was applied to the muscle cells of the ascaris body wall. the delay between the application of the peptide and the appearance of the response was measured and compared with that of gamma ... | 2002 | 12118721 |
| role of cysteine 306 in the catalytic mechanism of ascaris suum phosphoenolpyruvate carboxykinase. | biochemical and metabolic data lead to the conclusion that the enzyme phosphoenolpyruvate carboxykinase (pepck) contributes to a critical point of divergence in energy conservation pathways between mammals and nematodes. the ascaris suum pepck shares considerable homology with pepck from avian liver and is a good candidate for mutagenesis studies. the cys306 substitution by ser and ala produced active enzymes and the two mutants are kinetically indistinguishable from each other. this substitutio ... | 2002 | 12127066 |
| visceral larva migrans due to ascaris suum which presented with eosinophilic pneumonia and multiple intra-hepatic lesions with severe eosinophil infiltration--outbreak in a japanese area other than kyushu. | a 32-year-old man presented with the chief complaint of severe cough. examination of peripheral blood showed a marked increase in eosinophils. chest ct demonstrated multiple ground glass opacities in both lungs. bronchoalveolar lavage showed abundant eosinophils. abdominal ct demonstrated multiple low attenuation areas in the liver. liver biopsy with ultrasonography revealed severe eosinophil infiltrations around the portal veins. serologically, a multi-dot enzyme linked immunosorbent assay (dot ... | 2002 | 12132528 |
| purification and characterization of a novel glutathione s-transferase from asaphis dichotoma. | an isoenzyme of glutathione s-transferase (adgst) was purified from liver intestine of the seashell (asaphis dichotoma) by gst-sepharose 4b affinity chromatography followed by reverse-phase hplc. the enzyme has a pi value of 4.6 and is composed of two subunits each with a molecular weight of 23kda. it exhibits different catalytic activities toward the substrates 1-chloro-2,4-dinitrobenzene, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ethacrynic acid, and p-nitrophenyl acetate and, fascinatingly, sh ... | 2002 | 12139969 |
| immunomodulatory properties of ascaris suum glycosphingolipids - phosphorylcholine and non-phosphorylcholine-dependent effects. | immunomodulatory properties of phosphorylcholine (pc)-containing glycosphingolipids from ascaris suum were investigated utilizing immune cells from balb/c mice. proliferation of splenic b cells induced either via f(ab')2 fragments of anti-murine ig (anti-ig) or lps was significantly reduced when the glycosphingolipids were present in the culture medium. however whereas the lps-mediated effect was dependent on the pc moiety of the glycosphingolipids, the result generated when using anti-ig was no ... | 2002 | 12654088 |
| species-specific proteins identified in ascaris lumbricoides and ascaris suum using two-dimensional electrophoresis. | the protein profile of adult female ascaris lumbricoides and ascaris suum originating from humans and pigs, respectively, was studied using two-dimensional polyacrylamide gel electrophoresis. six different major protein spots specific for a. lumbricoides were identified irrespective of their geographical origin and no major specific spot was encountered in a. suum. no major differences in the protein profiles between the extract by phosphate-buffered saline and urea were encountered for either a ... | 2002 | 12172821 |
| effects of an eosinophil chemotaxis inhibitor, tak-661, on antigen-induced asthmatic responses in allergic sheep. | eosinophils have been shown to play a role in allergen-induced airway responses. the aim in this study was to examine the effects of tak-661, a newly developed product as a specific inhibitory agent of eosinophil chemotaxis, on antigen-induced asthmatic responses in allergic sheep model. seven ascaris-sensitive, "dual-respondent" allergic sheep were provocated by an ascaris suum antigen or phosphate-buffered saline 2 hrs after intra-stomach administration of tak-661 or a placebo. pulmonary resis ... | 2002 | 12177730 |
| eosionophilic pseudotumor of the liver due to ascaris suum infection. | a case of eosinophilic pseudotumor of the liver due to ascaris (a) suum is described in a 34-year-old-man with a high serum level of immunoglobulin e and hypereosinophilia ascribed to a history of atopic dermatitis since childhood. multiple hepatic hypoechoic nodules detected by ultrasound were confirmed as low-density nodules on computed tomography (ct), and as low and high signal intensity lesions on t1-and t2-weighted magnetic resonance imaging (mri), respectively. ct during arteriography (ct ... | 2002 | 12191679 |
| lung-stage protein profile and antigenic relationship between ascaris lumbricoides and ascaris suum. | the protein profile and antigenic properties of lung-stage larvae of ascaris lumbricoides and a. suum were studied using 2-dimensional electrophoresis and immunoblot analysis, respectively. the protein profiles of the 2 parasites were identical except for the presence of only 1 major protein spot specific for each. there was a complete cross-reactivity between the 2 parasites at the immunological level, and no specific antigen was recognized using specific antibody raised against the 2 parasites ... | 2002 | 12197147 |
| bioactivity studies on beta-sitosterol and its glucoside. | beta-sitosterol and beta-sitosteryl-beta-d-glucoside were isolated as analgesic constituents from the leaves of mentha cordifolia opiz. the acetic acid-induced writhing test showed that beta-sitosterol and beta-sitosteryl-beta-d-glucoside decreased the number of squirms induced by acetic acid by 70.0% and 73.0%, respectively, at a dose of 100 mg / kg mouse. statistical analysis using the kruskall wallis one-way analysis of variance by ranks showed that these isolates approximate the analgesic ac ... | 2002 | 12203259 |
| anthelmintic paraherquamides are cholinergic antagonists in gastrointestinal nematodes and mammals. | oxindole alkaloids in the paraherquamide/marcfortine family exhibit broad-spectrum anthelmintic activity that includes drug-resistant strains of nematodes. paraherquamide (phq), 2-deoxoparaherquamide (2dphq), and close structural analogs of these compounds rapidly induce flaccid paralysis in parasitic nematodes in vitro, without affecting adenosine triphosphate (atp) levels. the mechanism of action of this anthelmintic class was investigated using muscle tension and microelectrode recording tech ... | 2002 | 12213111 |
| abf-1 and abf-2, asabf-type antimicrobial peptide genes in caenorhabditis elegans. | two genes encoding the asabf (ascaris suum antibacterial factor)-type antimicrobial peptide, abf-1 and abf-2, were identified in caenorhabditis elegans. recombinant abf-2 exhibited potent microbicidal activity against gram-positive and gram-negative bacteria, and yeasts. the tissue-specific distribution estimated by immunofluorescence staining and transgenic analysis of a gfp fusion gene (where gfp corresponds to green fluorescent protein) suggested that abf-2 contributes to surface defence in t ... | 2002 | 11772394 |
| role of complex ii in anaerobic respiration of the parasite mitochondria from ascaris suum and plasmodium falciparum. | parasites have developed a variety of physiological functions necessary for existence within the specialized environment of the host. regarding energy metabolism, which is an essential factor for survival, parasites adapt to low oxygen tension in host mammals using metabolic systems that are very different from that of the host. the majority of parasites do not use the oxygen available within the host, but employ systems other than oxidative phosphorylation for atp synthesis. in addition, all pa ... | 2002 | 11803022 |
| [adaptation to low oxygen tension in parasite mitochondria]. | 2002 | 11808193 | |
| the mitochondrial genomes of the human hookworms, ancylostoma duodenale and necator americanus (nematoda: secernentea). | the complete mitochondrial genome sequences were determined for two species of human hookworms, ancylostoma duodenale (13,721 bp) and necator americanus (13,604 bp). the circular hookworm genomes are amongst the smallest reported to date for any metazoan organism. their relatively small size relates mainly to a reduced length in the at-rich region. both hookworm genomes encode 12 protein, two ribosomal rna and 22 transfer rna genes, but lack the atp synthetase subunit 8 gene, which is consistent ... | 2002 | 11812491 |
| how nematode sperm crawl. | sperm of the nematode, ascaris suum, crawl using lamellipodial protrusion, adhesion and retraction, a process analogous to the amoeboid motility of other eukaryotic cells. however, rather than employing an actin cytoskeleton to generate locomotion, nematode sperm use the major sperm protein (msp). moreover, nematode sperm lack detectable molecular motors or the battery of actin-binding proteins that characterize actin-based motility. the ascaris system provides a simple 'stripped down' version o ... | 2002 | 11839788 |
| electron-transfer complexes in ascaris mitochondria. | parasites have developed a variety of physiological functions necessary for their survival within the specialized environment of the host. using metabolic systems that are very different from those of the host, they can adapt to low oxygen tension present within the host animals. most parasites do not use the oxygen available within the host to generate atp, but rather employ anaerobic metabolic pathways. in addition, all parasites have a life cycle. in many cases, the parasite employs aerobic m ... | 2002 | 12238891 |
| alternative substrates for malic enzyme: oxidative decarboxylation of l-aspartate. | the nad-malic enzyme from ascaris suum will utilize l-aspartate, (2s,3r)-tartrate, and meso-tartrate as substrates with v/k values 10(-4)-10(-5) with respect to malate. there is a strict requirement for the 2s stereochemistry for all of these reactants. since aspartate is unique as an amino acid reactant for malic enzyme, it was informative to determine the details of its mechanism of oxidative decarboxylation. the initial rate of nadh appearance is directly proportional to the concentration of ... | 2002 | 12356322 |
| modulation of a heterologous immune response by the products of ascaris suum. | helminth infections are among the most potent stimulators of th2-type immune responses and have been widely demonstrated to modify responsiveness to both nonparasite antigens and other infectious agents in a nonspecific manner in infected animals. we investigated the immunomodulatory properties of pseudocoelomic body fluid from adult ascaris suum gastrointestinal helminths (abf) and its defined allergen (aba-1) by examining their effects on the immune response to a heterologous antigen, ovalbumi ... | 2002 | 12379682 |
| endothelin-induced vascular and bronchial effects in pig airways: role in acute allergic responses. | the effects of endothelin (et) agonists on airway mechanics and bronchial blood flow were studied as well as the effects of mixed et-receptor antagonist bosentan on allergen-induced airway reactions in the pig. et agonists [et-1, et-3, and the et(b) receptor-selective agonist sarafotoxin 6c (sf6c)] were given as intravenous injections (0.4-200 pmol/kg) to eight anesthetized pigs. bosentan (10 mg/kg iv) was then administered, and the injections were repeated. only sf6c caused a significant increa ... | 2002 | 12381744 |
| an embryo-associated fatty acid-binding protein in the filarial nematode brugia malayi. | brugia malayi is a filarial nematode parasite that causes lymphatic filariasis, a disease that affects millions of people in the tropics. sexual reproduction of filarial worms occurs within the lymphatic vessels of the human host and is crucial for transmission of the parasite to the mosquito vector. we have previously identified several b. malayi genes that exhibit apparent gender-specific expression. one of these had significant sequence similarity to the ascaris suum embryo-associated fatty a ... | 2002 | 12387845 |
| secretion of a novel developmentally regulated chitinase (family 19 glycosyl hydrolase) into the perivitelline fluid of the parasitic nematode, ascaris suum. | the early development of the parasitic nematode, ascaris suum, occurs within a chitinous eggshell and an abundant chitinase (as-p50) has been identified in the perivitelline fluid (pvf) surrounding the infective larva prior to hatching. a cdna encoding as-p50 was cloned, sequenced and the protein expressed in escherichia coli. as-p50 is a member of glycosyl hydrolase family 19, previously identified only in plants, making the characterization of as-p50 the first family 19 glycosyl hydrolase from ... | 2002 | 12387846 |
| haemoconcentration associated with low vitamin a status can mask anaemia. | as part of a pig study to elucidate the interactions between low vitamin a status and helminth infections, surprisingly, we observed higher haemoglobin levels and packed cell volumes in the pigs with low vitamin a status. a possible haemoconcentration effect, due to some disturbance in the regulation of the extracellular fluid volume, could lead to underestimation of the prevalence of anaemia in vitamin a deficient human populations. therefore, this phenomenon needs to be further clarified in st ... | 2002 | 12387911 |
| montelukast prevents antigen-induced mucociliary dysfunction in sheep. | the cysteinyl leukotrienes are potent proinflammatory mediators that, in addition to their bronchospastic actions, can also contribute to mucociliary dysfunction, a central component of the pathophysiology of asthma. in this study, we determined whether montelukast, a cysteinyl leukotriene 1 receptor antagonist, could prevent and/or reverse antigen-induced mucociliary dysfunction in allergic sheep. we measured tracheal mucus velocity, a marker of mucociliary clearance, before and for 8 hours aft ... | 2002 | 12406819 |
| functional characterization of alternatively spliced 5-ht2 receptor isoforms from the pharynx and muscle of the parasitic nematode, ascaris suum. | serotonin (5-ht) receptors play key regulatory roles in nematodes and alternatively spliced 5-ht2 receptor isoforms have been identified in the parasitic nematode, ascaris suum. 5-ht2as1 and 5-ht2as2 contain different c-termini, and 5-ht2as1delta4 lacks 42 amino acids at the c-terminus of the third intracellular loop. 5-ht2as1 and 5-ht2as2 exhibited identical pharmacological profiles when stably expressed in human embryonic kidney (hek) 293 cells. both 5-ht2as isoforms had higher affinity for 5- ... | 2002 | 12423236 |
| characterization of sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blotting analysis. | sm14 was the first fatty acid-binding protein homologue identified in helminths. thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. in addition, immune crossprotection was also reported against fasciola hepatica infection, in animals previously immunized with the schistosoma mansoni vaccine candidate, r-sm14. in the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ... | 2002 | 12426606 |
| antimicrobial peptides and protease inhibitors in the skin secretions of the crawfish frog, rana areolata. | the dorsal skin of the crawfish frog, rana areolata, is associated with numerous prominent granular glands. proteomic analysis of electrically stimulated skin secretions from these glands enabled the identification and characterization of eight peptides with antimicrobial and hemolytic activity belonging to the previously identified brevinin-1, temporin-1, palustrin-2, palustrin-3, esculentin-1 (two peptides), and ranatuerin-2 (two peptides) families. the primary structures of the peptides were ... | 2002 | 12429503 |
| minor participation of camp on the protein kinase phosphorylation of mitochondrial and cytosolic fractions from ascaris suum: a comparative study with porcine heart muscle. | in contrast to porcine heart muscle in which camp effectively activated the phosphorylation of cytosolic proteins, camp exerted a minor effect on the phosphorylation of proteins from the soluble fraction of ascaris suum muscle. similarly, camp did not enhance the kinase activity in the mitochondrial membranes from porcine heart and a. suum, although major differences in protein phosphorylation were observed between both fractions. however, camp-dependent protein kinases (pka) were evidenced in t ... | 2002 | 12431408 |
| levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in ascaris suum suggest that cam kinase and tyrosine kinase regulate sensitivity to levamisole. | a two-micropipette current-clamp technique was used to record electrophysiological responses from the somatic muscle of ascaris suum. levamisole and acetylcholine were applied to the bag region of the muscle using a microperfusion system. depolarizations produced by 10 s applications of 10 micro mol l(-1) levamisole or 20 s applications of 10 micro mol l(-1) acetylcholine were recorded. the effect on the peak membrane potential change of the kinase antagonists h-7, staurosporine, kn-93 and genis ... | 2002 | 12432019 |
| synthetic studies on glycosphingolipids from the protostomia phyla: syntheses of arthro-series glycosphingolipids. | glycosphingolipids isolated from larvae of the green-bottle fly, lucilia caesar, have quite unique structures containing glcnacbeta-(1 --> 3)-man and galnacbeta-(1 --> 4)-glcnacbeta-(1 --> 3)-man. we have synthesized two glycosphingolipids, beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer and beta-d-galnacp-(1 --> 4)-beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer. a key reaction in the synthetic sequence is the application of the intramolecular a ... | 2002 | 12433469 |
| synthesis and nematocidal activities of n-(2-methylpropyl)-3-(x-phenyl)-2e-propenamides. | 2002 | 12434551 | |
| pf4, a fmrfamide-related peptide, gates low-conductance cl(-) channels in ascaris suum. | here we describe the actions of the peptide lys-pro-asn-phe-ile-arg-phe-nh(2), or pf4, on inside-out membrane patches (n=164), recorded from vesicles derived from ascaris suum somatic muscle cells. we observed numerous, small-amplitude cl(-) channels in the membrane patches. the conductance of the cl(-) channels ranged from 1.09 to 7.07 ps, the open probability (p(open)) ranged from 0.047+/-0.015 (mean+/-s.e.m.) at 0 microm pf4 to 0.156+/-0.026 at 0.1 microm pf4. the channel mean open time was m ... | 2002 | 12450564 |
| cloning and characterisation of a highly immunoreactive 37 kda antigen with multi-immunoglobulin domains from the swine roundworm ascaris suum. | antigens from larval stages of ascaris suum have been shown to induce protection against challenge infection with infective a. suum eggs. we previously identified several antigens that reacted strongly with serum from pigs inoculated with infective eggs containing l3. in this study, we isolated an antigen with a molecular mass of 37 kda and a pi of 4.8 (as37) from a. suum infective eggs using two-dimensional electrophoresis, and obtained a full-length cdna by reverse transcription-polymerase cha ... | 2002 | 12464420 |
| structure-activity relationships of an inhibitory nematode fmrfamide-related peptide, sdpnflrfamide (pf1), on ascaris suum muscle. | fmrfamide-related peptides are widespread among the nematoda. among them is a family of extended pnflrfamide peptides encoded on the flp-1 peptide precursor gene in caenorhabditis elegans. the most studied peptide from this series is sdpnflrfamide (pf1). each residue in this peptide was sequentially substituted with either alanine or the corresponding d-isomer of the native amino acid in order to define structure-function relationships in this peptide using an ascaris suum muscle tension assay. ... | 2002 | 12464423 |
| role of dihydrolipoyl dehydrogenase (e3) and a novel e3-binding protein in the nadh sensitivity of the pyruvate dehydrogenase complex from anaerobic mitochondria of the parasitic nematode, ascaris suum. | the pyruvate dehydrogenase complex (pdc) plays changing roles during the aerobic-anaerobic transition in the life cycle of the parasitic nematode, ascaris suum. however, the dihydrolipoyl dehydrogenase (e3) subunit appears to be identical in all stages, despite the fact that the pdc is less sensitive to nadh inhibition in anaerobic muscle. therefore, we have cloned cdnas encoding e3 and a novel anaerobic-specific e3-binding protein (e3bp) that lacks the terminal lipoyl domain found in e3bps from ... | 2002 | 12467981 |
| developmental abnormalities in chicken embryos after injection proteolysis inhibitors from ascaris suum. | it has been found that trypsin and alpha-chymotrypsin inhibitors isolated from ascaris suum act embryotoxically and teratogenically on white leghorn chicken embryos. mortality rate for the chicken embryos on day 15 of incubation was 45.0 +/- 3.5% after injection of trypsin inhibitor and 44.0 +/- 3.5% after adminstration alpha-chymotrypsin inhibitor. gross examination of surviving embryos and their dissection revealed pathological changes (abdominal dropsy, umbilical hernia, subcutaneous oedema, ... | 2002 | 16883703 |
| a simple 1-d physical model for the crawling nematode sperm cell. | we develop a one-dimensional physical model of the crawling movement of simple cells: the sperm of a nematode, ascaris suum. the model is based on the assumptions that polymerization and bundling of the cytoskeletal filaments generate the force for extension at the front, and that energy stored in the gel formed from the filament bundles is subsequently used to produce the contraction that pulls the rear of the cell forward. the model combines the mechanics of protrusion and contraction with che ... | 2003 | 19277214 |
| preliminary evaluation of maternotoxic effect of ascaris extract in mice. | administration intraperitoneally of the ascaris suum extract--ase-(0.6-1.4 g of ascaris proteins/kg/day) at a late stage of organogenesis (8-12 days of gestation) disturbed course of mouse pregnancy. it has been found that injections of higher doses of ase to pregnant mice caused the symptoms manifesting maternal toxicity (decreased body weight gain/p < 0.001/as compared to control, intrauterine resorption of litter, vaginal hemorrhages, female mortality and altered behaviour). there is a linear ... | 2003 | 16889025 |
| a comparison of the susceptibility of growing mukota and large white pigs to infection with ascaris suum. | the influence of a. suum infection on the haematology, liver-related serum enzymes, blood urea and live weight gain in mukota and large white (lw) weaner pigs was compared. six pigs of each genotype were infected with a single dose of 4000 a. suum eggs per pig and another six were not. the pigs were kept for 100 days. blood was collected daily for the first 7 days and also after 100 days. in the infected pigs, there was an increase (p<0.05) in alanine aminotransferase (alt) activity in the lw bu ... | 2003 | 14672454 |
| de novo sequencing of novel neuropeptides directly from ascaris suum tissue using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight. | direct analysis of tissue by matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) allows for the rapid profiling of biological molecules with minimal loss of sample or degradation and reduced likelihood of chemical modification. however, there are still considerable challenges to overcome due to the complexity of tissue and the low quantity of endogenous peptide in a single cell. these problems are exacerbated in the nematode ascaris suum because of the small size of individu ... | 2003 | 14673815 |
| in vitro studies on the effects of flubendazole against toxocara canis and ascaris suum. | adult toxocara canis and ascaris suum were incubated in vitro in media containing 0.1, 1, 10 or 100 micro g/ml flubendazole in order to study drug-derived effects. this incubation was done for 8 h and repeated (in some groups) after 24 h for another 8 h. the onset and intensity of flubendazole-derived effects were dosage-dependent and time-dependent, i.e. the same grade of damage was reached when incubating for a longer period at a low dosage or for a shorter period in medium containing a high a ... | 2003 | 12474046 |
| structural elucidation of zwitterionic carbohydrates derived from glycosphingolipids of the porcine parasitic nematode ascaris suum. | carbohydrates substituted with phosphocholine (pc) and phosphoethanolamine (pe) were released from zwitterionic glycosphingolipids of the pig parasitic nematode ascaris suum by treatment with endoglycoceramidase. individual glycans were obtained by hplc on porous graphitic carbon followed by high-ph anion-exchange chromatography. in addition to the known pentasaccharides gal alpha 3galnac beta 4[pc6]glcnac beta 3man beta 4glc and gal alpha 3galnac beta 4[pc6]glcnac beta 3[pe6]man beta 4glc, the ... | 2003 | 12234251 |
| critical role of il-5 in antigen-induced pulmonary eosinophilia, but not in lymphocyte activation. | infiltration of antigen-specific cd4+ t cells and production of interleukin (il)-5 in inflammatory regions play a central role in antigen-induced pulmonary eosinophilia. genetically il-5-deficient mice lack antigen-induced airway eosinophilia, but little is known about the role of il-5 in accumulation and activation of cd4+ t cells in the lung and in airway hyperresponsiveness (ahr). ascaris suum extract (asc) has been used to induce airway eosinophilia and analyze airway inflammation in il-5 re ... | 2003 | 12660425 |
| augmentation by bursaphelenchus xylophilus, a pine wood nematode, of polyclonal ige production induced by lipopolysaccharide plus interleukin-4 in murine splenocytes. | bursaphelenchus xylophilus (b. xylophilus) is a pine wood nematode that is known to cause pine wilt disease. we report here that b. xylophilus extracts augmented the polyclonal immunoglobulin e (ige) production induced by lipopolysaccharide (lps) plus interleukin-4 (il-4) both in murine splenocytes and purified b cells as determined by elisa and elispot assays, but they did not cause such a promotion in the absence of either lps or il-4. we also observed that the antigen-nonspecific ige levels w ... | 2003 | 12686761 |
| evolution of ascariasis in humans and pigs: a multi-disciplinary approach. | the nematode parasite ascaris lumbricoides infects the digestive tracts of over 1.4 billion people worldwide, and its sister species, ascaris suum, has infected a countless number of domesticated and feral pigs. it is generally thought that the putative ancestor to these worms infected either humans or pigs, but with the advent of domestication, they had ample opportunity to jump to a new host and subsequently specialize and evolve into a new species. while nuclear dna makers decisively separate ... | 2003 | 12687761 |
| precision and accuracy of an assay for detecting ascaris eggs in various biosolid matrices. | this paper presents quality assurance data and quality control data on the recovery of ascaris suum eggs from various biosolid matrices: acid-treated, alkaline-treated, amended-soil blended, and lagoon stored biosolids. over a period of years, the same procedure, the "tulane method," was performed on different matrices, and in this work, the data collected on the recovery of the eggs from the different matrices is examined and compared. the egg recoveries are discussed in terms of precision (the ... | 2003 | 12691891 |
| the polyprotein and far lipid binding proteins of nematodes: shape and monomer/dimer states in ligand-free and bound forms. | nematodes produce two classes of small, helix-rich fatty acid- and retinol-binding proteins whose structures and in vivo functions remain to be elucidated. these are the polyprotein allergens (npa) and the far proteins. the solution properties of recombinant forms of these proteins from parasitic [ascaris suum (as) and onchocerca volvulus (ov)] and free-living [caenorhabditis elegans (ce)] nematodes have been examined. analytical ultracentrifugation (auc) showed that, contrary to previous findin ... | 2003 | 12692694 |
| common structural properties specifically found in the csalphabeta-type antimicrobial peptides in nematodes and mollusks: evidence for the same evolutionary origin? | the structural properties of the ascaris suum antibacterial factor (asabf)-type antimicrobial peptides, isolated from nematodes, were compared with the csalphabeta-type antimicrobial peptides found in other organisms. the spacing of the half-cystine residues, cysteine pairings, and organization of the precursor were different from the 'classical' csalphabeta-type antimicrobial peptides, such as drosomycin and plant defensins, and identical only to the mgd and myticin in mollusks. in addition, ab ... | 2003 | 12697306 |