Publications

renal transplant-associated malaria.		1980	6999187
labelling of membrane glycoproteins of cultivated plasmodium falciparum.	membrane glycoprotein synthesis by plasmodium falciparum was determined by metabolic labelling in the presence of 74 kbq/ml (2.5 muci/ml) glucosamine-(3)h. five major glycoprotein bands and four minor ones were demonstrated. a control experiment using normal, outdated, human erythrocytes indicates that there was no incorporation of the labelled glucosamine into the erythrocyte membrane. it was also demonstrated that the rate of membrane glycoprotein synthesis by mature parasites of the trophozoi ...	1980	6998592
diagnosis of plasmodium falciparum infection using a solid-phase radioimmunoassay for the detection of malaria antigens.	a serodiagnostic test has been developed for the detection of plasmodium falciparum in infected blood. using parasite antigens and infected red blood cells from in vitro cultures of p. falciparum and malaria antibody from high-titre gambian sera, parasites were detected in a solid-phase radioimmunoassay (ria) that measured antibody-binding inhibition. lysed rbc were incubated with labelled igg purified from immune sera and were then placed in antigen-coated microtubes and incubated. the degree o ...	1980	6998591
a longitudinal study of plasmodium falciparum malaria in the west african savannah using the elisa technique.	malarial antibody levels were measured by the enzyme-linked immunosorbent assay (elisa) in two west african populations, one exposed to intense malaria transmission and the other protected. the results reflected the transmission of maternal antibody and, in the unprotected population, the subsequent increase of the elisa values with age reflected the development of the immune response to malaria. malaria control activities reduced elisa values in the protected population. the limitations of the ...	1980	6998590
stage-specific proteins and glycoproteins of plasmodium falciparum: identification of antigens unique to schizonts and merozoites.	establishment of highly synchronized cultures of plasmodium falciparum enabled identification of stage-specific proteins, glycoproteins, and antigens. comparison of metabolically labeled constituents of rings, trophozoites, mature schizonts, and merozoites indicated the absence of major proteins or glycorproteins unique to rings or trophozoites. a burst of new synthetic activity occurred during schizogony when several schizont- and merozoite-specific proteins and glycoproteins became apparent. i ...	1980	6997881
[acute respiratory insufficiency as a complication of falciparum malaria].		1980	6997762
study of plasmodium falciparum resistance to 4-aminoquinolines (chloroquine) in sabah, malaysia.	the status of p. falciparum resistance to chloroquine in sabah, malaysia were not know until 1971-1972. several in-vivo and on in-vivo studies were conducted from 971-1975, and the result showed 51% out of total 57 cases studied were resistant to chloroquine. the latest in-vitro study (collaborative with who) started in july 1978, to continue till 1980, to cover the whole state. the preliminary result shows 65 cases (85%) out of a total 76 successful tests are resistant to chloroquine. on the ba ...	1980	7003166
malaria: a laboratory risk.	a case is described of malaria contracted in a clinical laboratory by accidental self-inoculation with infected blood.	1980	7003569
effect of a single dose of primaquine on a thai strain of plasmodium falciparum.	a single dose of 45 mg of primaquine base was effective in the elimination of gametocytes of the possibly chloroquine-resistant p. falciparum in thailand. the gametocyte counts decreased markedly within 3.5 days showing significantly difference from the control in which mostly gametocytes still remain within 11.4 days. the development of the gametocytes in a. dirus was not observed after treatment. primaquine can be used as gametocytocide and sporontocide for falciparum malaria in thailand if th ...	1980	7003735
adaptation of cultured plasmodium falciparum to the intact squirrel monkey (saimiri sciureus).		1980	7003834
the development and validation of an enzyme linked immunosorbent assay for malaria.	elisa has been evaluated and validated for malaria. it is sensitive, specific and reproducible. a comparison of elisa and ifat has been made, and there is a good correlation between the two tests. elisa results can be assessed objectively. a small study on caucasians with clinical malaria shows the value of elisa as an immunodiagnostic tool. its seroepidemiological value is also demonstrated, since it quickly reflects the changes in malaria transmission after a who malaria control programme in a ...	1980	7003852
[acute pulmonary edema in cerebral malaria (author's transl)].		1980	6995935
[malaria: factors affecting the prognosis of falciparum malaria].		1980	6993728
effect of sodium cyanate on plasmodium falciparum in vitro.	sodium cyanate at concentrations as low as 0.5 mm inhibited the growth of plasmodium falciparum (fcr-3 strain) utilizing the trager-jensen continuous culture system. at concentration higher than 1 mm, the parasites were irreversibly destroyed. utilizing synchronized cultures, the relative susceptibilities of early and late trophozite forms were examined, and it was found that both developmental forms were equally susceptible to the action of cyanate. pretreatment of red cells with sodium cyanat ...	1980	6993647
peptones and calf serum as a replacement for human serum in the cultivation of plasmodium falciparum.	attempts were made to find an inexpensive, readily available substitute for human serum requirement for the continuous culture of erythrocytic stages of plasmodium falciparum. we found that neopeptone or proteose-peptone no. 3 added together with calf serum gave parasite growth rates comparable to, or surpassing, those obtained with human serum. however, first it was necessary to adapt the parasites by a gradual, stepwise reduction in the amount of human serum used, and a concomitant, stepwise i ...	1980	6993641
malaria in vietnamese refugees.		1980	7004613
[a case of chloroquine-resistant (r1) falciparum malaria from the east african comoros islands (author's transl)].	an african from the comoros islands, who has been living in berlin for eleven years and who had made his last journey to africa two years before, fell ill with high fever upon returning from a visit to mayotte and grande comore. his blood smear revealed trophozoites of plasmodium falciparum. after intake of 25 mg of chloroquine base per kg body weight within 66 hours, defervescence and clearance of blood ensued and by the fourth day after start of treatment the parasites could no longer be detec ...	1980	6993367
factors contributing to the prognosis of falciparum malaria.	factors contributing to the poor outcome of imported falciparum malaria in adult patients were investigated and following conclusions were drawn. 1) in the case of primarily non-immune patients, a catastrophic course of the disease was precipitated when the inception of treatment was delayed beyond 6 days after the onset of illness. nine fatal and 3 moribund cases presently reported fell in this category. however, there were exceptional cases who made an uneventful recovery, despite the delay i ...	1980	7005492
population studies in cameroon: hemoglobin s, glucose-6-phosphate dehydrogenase deficiency and falciparum malaria.	examination of blood samples from 1,183 individuals from cameroon indicates that sickle cell trait frequencies and g6pd deficiency frequencies were heterogeneous among villages as well as within geographic areas and ethnic groups. mean parasite counts were significantly correlated with hb as frequencies for children 6 years of age and under, although no correlation was found for mean parasite counts and g6pd deficiency frequencies. the mean age of sickle cell trait individuals was found to be si ...	1980	6993342
congenital malaria due to plasmodium falciparum.	malaria manifested during the first few months of life may be result of acquisition during pregnancy, at the time of delivery, or by mosquito bite after birth. both congenital and perinatal malaria are acquired by the transmission of parasitized maternal erythrocytes across the placenta. an infant is described whose mother was diagnosed to have malaria at six months of gestation. the infant developed intermittent fever at 5 weeks of age and presented with anemia and hepatosplenomegaly at 3 month ...	1980	7005857
[post-transfusional malaria: a case of double infection with plasmodium falciparum and plasmodium malariae].		1980	7006037
the world health organisation north fly clinico-epidemilogical pilot study.		1980	7006244
erythrocyte g-6-pd and 6-pgd genetic polymorphisms in south african negroes, with a note on g-6-pd and the malaria hypothesis.	sample of 981 and 998 south african negroes belonging to seven different ethnic groups were screened for g-6-pd and 6-pgd phenotypes, respectively. the results are discussed in terms of the interethnic variability and the possible adaptive values of these genetic polymorphisms. particular attention is paid to the geographic co-distribution and interrelationship of g-6-pd deficiency and the occurrence of malaria in south africa.	1980	6993340
[in vitro study of the sensitivity of plasmodium falciparum to chloroquine].	in vitro testing of p. falciparum chemosensitivity was assessed from 2 severe malaria cases in which the parasite was thought to be resistant to chloroquine. the 3 following methods were compared in both cases. 1.--maturation test, carried out before and from the outset of treatment, is a rapid and simple method. primary efficiency of therapeutic procedures can be estimate and the eventually adjust. 2.--rieckmann test, on patient blood, measure chemosensitivity of host strain modulated by hosts ...	1980	7006839
[post-transfusion malaria (plasmodium falciparum) and carcinoma under chemotherapy: unusual cytology of the parasite].	a man with a carcinoma presented an unusual cytology p. falciparum transfusion malaria. the authors suspect cytotoxic chemotherapy for having induced these cytological changes.	1980	7006840
direct coombs antiglobulin reactions in gambian children with plasmodium falciparum malaria. ii. specificity of erythrocyte-bound igg.	the cause of direct coombs (dat) positivity in west african (gambian) children has been investigated. results obtained from a comparison of two groups of primary school children, one from an area hyperendemic for p. falciparum malaria and the other partially protected from malaria, indicate an association between a high incidence of dat positivity and p. falciparum parasitaemia with raised serum antibody titres to falciparum schizonts. erythrocytes taken from children with a high igg dat titre w ...	1980	6993068
chloroquine resistance in the south-west pacific.		1980	7007842
plasmodium falciparum in vitro: determination of chloroquine sensitivity of three new strains by a modified 48-hour test.	three recently isolated african strains of plasmodium falciparum were tested in vitro for their response to chloroquine. both the 48-hour method described earlier and a modified 48-hour test were used, yielding comparable results. strain fcr-7/kenya, isolated from a clinically chloroquine-resistant case, was more resistant to the drug in vitro than the two other strains (fcr-8/west africa and fcn-1/nigeria, both isolated from chloroquine-sensitive cases). complete inhibition of parasite growth o ...	1980	6992605
cultivating friends and parasites.		1980	6992604
homology between a histidine-rich protein from plasmodium lophurae and a protein associated with the knob-like protrusions on membranes of erythrocytes infected with plasmodium falciparum.	the incorporation of several radioactive amino acids into the knob protein of plasmodium falciparum was compared. histidine showed better incorporation than proline. a protein hydrolysate, which had all major amino acids except histidine and methionine, showed relatively poor incorporation as compared with proline, and no labeling could be detected with methionine or leucine. these results strongly suggest that the amino acid composition of the knob protein has the same peculiarities as that of ...	1980	6991629
malaria and growth stunting in young children of the highlands of papua new guinea.	in a highlands valley, at low altitude, malaria is a contributing factor to stunting of growth, an expression of chronic malnutrition, in young children. the affect is most marked in children under two years of age, and may result from retarded intrauterine growth, although malaria also possibly exerts a direct affect on growth in young children. in the absence of a malaria control programme, distribution of amodiaquine to young children and chloroquine to pregnant women and mothers of young chi ...	1980	7008427
elimination of yeast contamination from malaria cultures using 5-fluorocytosine.		1980	7008723
anti-lymphocytotoxic antibodies in sera of thai adults infected with plasmodium falciparum or plasmodium vivax.	because of the potential for the elimination of lymphocytes through anti-lymphocytotoxic antibodies we examined individual sera of patients infected with falciparum or vivax malaria for the presence of antibodies against normal peripheral blood mononuclear cells. in assays done at 15 degrees c, 95% of the p. falciparum patients and 98% of the p. vivax patients showed evidence for antibody activity. activity at 37 degrees c was significantly less than that at 15 degrees c. these studies suggest t ...	1980	6991173
[importation of malaria from abroad into the ussr (1974-1979)].		1980	7010104
falciparum malaria in minnesota.		1980	6990226
recent advances concerning the use of muramyl dipeptide derivatives as vaccine potentiators.		1980	7010376
infection of anopheles freeborni by gametocytes of cultured plasmodium falciparum.		1980	7010690
the interaction between sickle haemoglobin and the malarial parasite plasmodium falciparum.	the mechanism whereby heterozygous carriers of the sickle cell gene are protected against fatal malarial infections due to plasmodium falciparum has been examined in a short term in vitro cultivation system. the results have shown that both parasite invasion of red cells and parasite growth within red cells containing sickle haemoglobin (hb-s) is restricted, but only under conditions of low (5%) oxygen tensions. to bring this about, the cells containing hb-s need not sickle. furthermore the grow ...	1980	7010693
studies on the use of a membrane feeding technique for infecting anopheles gambiae with plasmodium falciparum.	an investigation was made of the optimum feeding time when using stirred membrane feeders for infecting anopheles gambiae s.s. with plasmodium falciparum. the aim was to stimulate the natural situation of direct feeding on a host and so enable the malaria susceptibility of different populations of a. gambiae to be assessed. 45 feeds were carried out on blood donated by p. falciparum gametocyte carriers. the results show that only patients with at least 300 gametocytes/mm3 are likely to produce a ...	1980	7010696
in vitro microtechnique for determining the drug susceptibility of cultured parasites of plasmodium falciparum.		1980	7010702
plasmodium falciparum and plasmodium vivax infections in saudi arabia, with a note on the distribution of anopheline vectors.	variations in the intensity of plasmodium infections were observed in the surveyed regions of saudi arabia. the highest incidence had been recorded from the southern region and the lowest form the northern and eastern regions. the western region, however, showed a regular and uniform occurrence of infection throughout the year. five anopheline vectors were identified in the country.	1980	7001040
isolation of plasmodium falciparum merozoites from cultivated schizonts bound to concanavalin a.		1980	7002077
experimental infection of the squirrel monkey (saimiri sciureus) with plasmodium falciparum.	seventy-six squirrel monkeys, saimiri sciureus, caught in guyana and french guiana, were examined for their susceptibility to infection with a strain of plasmodium falciparum. twenty-nine serial transfers were performed, both in splenectomized and intact animals. after a period of "adaptation," the strain became more virulent for the squirrel monkey and high parasitemias were obtained in splenectomized animals. frequently, such infections led to the death of infected animals. in intact squirrel ...	1980	7012286
seroepidemiology of malaria in peninsular malaysia: plasmodium falciparum antibody profile of adults in four states.	in 1973, 2610 sera were collected from adults living in 22 localities in four states in peninsular malaysia and tested by ifat for plasmodium falciparum antibodies. a larger number of thin films were examined. the attack phase of the malaria eradication programme (mep) in these areas was started between 1968 and 1973. the results showed that the highest prevalence rates and geometrical mean reciprocal titres (cmrt) were among adults from kelantan where the antibody prevalence varied greatly amon ...	1980	7001690
chloroquine sensitivity of plasmodium falciparum in ibadan, nigeria.	thirty-five children with plasmodium falciparum malaria were treated with 25 mg/kg chloroquine over three days and observed for seven days during which blood films were examined daily for malaria parasites. asexual forms of p. falciparum which were present in the blood films of all the patients before commencing treatment disappeared rapidly from the blood so that by the third day no parasites were seen in the blood film. the blood films remained negative for the rest of the seven-day observatio ...	1980	7001691
distribution of plasmodium falciparum in west bengal.		1980	7001693
specific antibody-dependent cellular cytotoxicity in human malaria.	a micromethod for the study of specific antibody-dependent cellular cytotoxicity (adcc) in human malaria is described, using cultured, asexual plasmodium falciparum parasites as viable target cells. lymphocytes from children with acute malaria, uninfected immune adult gambians and adult gambians infected with p. falciparum were capable of killing p. falciparum in vitro in the presence of malaria antibody. a parasite growth-promoting factor, produced by lymphocytes in non-immune serum and at a ly ...	1980	7002397
canine babesiosis: indirect fluorescent antibody test for a north american isolate of babesia gibsoni.	a sensitive and reproducible indirect fluorescent antibody test for a north american isolate of babesia gibsoni was described. in blind tests (coded samples), titer reproducibility was within two 4-fold dilutions and the appropriate positive and negative sera were identified as such. babesia gibsoni antisera reacted with homologous antigen at one to three 4-fold dilutions greater than with the following heterologous species: b bigemina, b argentina, b equi, plasmodium falciparum, and p brasilian ...	1980	7011131
[renal disorders during plasmodium falciparum induced acute malaria (author's transl)].		1980	7011155
a clinical trial of malaria prophylaxis using a single dose of chloroquine at different intervals in an endemic malarious area.	a controlled trial of chloroquine prophylaxis was carried out to find whether this drug could be administered at intervals longer than the conventional ones recommended, in endemic malarious areas. statistically significant results obtained show that chloroquine at suitable doses could be used successfully at intervals of 3 months. the possibility of chloroquine and acquired immunity acting together synergistically is discussed. the author is of the opinion that with this dose regime, the commun ...	1980	7001039
a case of p. falciparum malaria in thailand apparently resistant to quinine.		1980	7002075
plasmodium falciparum resistance to pyrimethamine and chlorproguanil -- host or parasite dependent?		1980	7002076
pyrimethamine sensitivity in plasmodium falciparum: determination in vitro by a modified 48-hour test.	four strains of plasmodium falciparum recently isolated in culture were assessed in vitro for their response to pyrimethamine. a simple modified 48-hour test was used, which showed two strains to be sensitive to the drug in vitro, while the other two were resistant at a very high level. in the two strains for which relevant clinical information was available the in vivo response to pyrimethamine was corroborated by the in vitro findings. this modified 48-hour test is thus useful for determining ...	1980	7011588
[clinico-anatomical conference of the pitié-salpêtrière hospital. case no. 8 - 1980].		1980	7013601
the increased susceptibility of young red cells to invasion by the malarial parasite plasmodium falciparum.	the relationship between red cell age and susceptibility to invasion by the malarial parasite plasmodium falciparum has been examined by several different methods including short-term cultures of parasitized human blood. the results indicate that reticulocytes and young red cells are more susceptible to invasion by this parasite as compared with metabolically older cell populations. this is contrary to the current belief that red cells of all ages are invaded indiscriminately by p. falciparum. t ...	1980	7002199
response to 600 mg amodiaquine base with 45 mg primaquine as presumptive treatment in chloroquine resistant plasmodium falciparum infection in assam.		1980	7014425
pyrimethamine susceptibility status of p. falciparum in karbi-anglong district of assam and adilabad district of andhra pradesh.		1980	7014699
direct antiglobulin reactions in gambian children with p. falciparum malaria. iii. expression of igg subclass determinants and genetic markers and association with anaemia.	the igg subclass and gm allotype distribution of red cell-bound igg molecules from gambian children with past or present falciparum malaria has been determined. the results show that the antibody is polyclonal with some predominance of the igg2 and igg4 subclasses. a restriction towards igg2 antibodies may indicate specificity for a schizont-derived antigen which is carbohydrate in nature. not all the allotypes normally carried by the g3m(b) allele could be demonstrated on igg3-sensitized cells, ...	1980	7002393
chemosuppressive field trials in thailand. iv. the suppression of plasmodium falciparum and plasmodium vivax parasitemias by mefloquine (wr 142,490, a 4-quinolinemethanol).	the effect of various dosages of mefloquine hydrochloride (wr 142,490) and sulfadoxine-pyrimethamine in the suppression of malaria infections was studied in an area of northeastern thailand highly endemic for both chloroquine-resistant plasmodium falciparum and for p. vivax. both preparations, in all regimens studied, were effective in greatly reducing the incidence of falciparum infections. mefloquine was more active in preventing vivax parasitemia than sulfadoxine-pyrimethamine; however, this ...	1980	7004214
plasmodium falciparum in vitro culture: improvements using umbilical cord serum and medium modifications.	umbilical cord serum is a cheap, readily available source of human serum which promotes in vitro p. falciparum growth as well or even better than adult serum, and gives more consistent results. by increasing to 35 mm the hepes concentration in rpmi medium, the time elapsed between two medium changes can be extended to 48 hours (or even to 72 hours if optimum multiplication rates are not required). an initial parasitemia of 0.2% with medium changes on day 2 and 3, generally results in 6 to 12% pa ...	1980	7015634
comparative studies of three strains of plasmodium falciparum isolated by the culture method of trager and jensen.	the ease which plasmodium falciparum parasites adapt to culture by the method of trager-jensen is variable. the strain characteristics of the first three isolates we cultured differed markedly, depending on the ease of adaptation. the west african i, which was cultured readily, proved to be sensitive to all antimalarials assessed by an in-vitro method, caused benign infections in aotus monkeys, and produced gametocytes both in the cultures and in aotus monkeys. in contrast, parasites of the libe ...	1980	7004215
treatment of falciparum malaria with sulfalene-pyrimethamine versus sulfadoxine-pyrimethamine.	the study was carried out in 89 uncomplicated falciparum malaria adult cases admitted to paholpol-phayuhasena hospital, kanchanaburi province, thailand, during july 1979 and march 1980. the patients were divided alternatively into 2 groups. group i, 46 patients, were treated with a single dose of 1000 mg sulfalene and 50 mg pyrimethamine (2 tablets of metakelfin). group ii, 43 patients, were treated with 1000 mg sulfadoxine and 50 mg pyrimethamine (2 tablets of fansidar). the parasitemia was cle ...	1980	7013094
separation of stages of plasmodium falciparum-infected cells by means of a fluorescence-activated cell sorter.	plasmodium falciparum parasites from long-term in vitro culture have been labeled with the dna-binding dye hoechst 33258. after labeling, parasitized cells have been successfully analyzed and sorted, using a fluorescence-activated cell sorter, into populations of uninfected, singly infected, and multiply infected cells.	1980	7004216
[prospects for the future in the control of malaria].		1980	7004580
malaria prophylaxis in travelers.	each year increasing numbers of americans travel to countries where the risk of acquiring malaria is considerable. appropriate advice from family physicians about malaria prophylaxis is important to prevent malaria in american travelers. all travelers to malarious areas should receive chloroquine prophylaxis. physicians should consider the diagnosis of malaria when fever develops in travelers returning from malarious areas.	1980	7005388
breeding of aotus monkeys for human malaria research.		1980	7006719
[a case of malaric encephalopathy (author's transl)].		1980	7008129
[malaria: recent clinical experience in the eastern townships].		1980	7008317
chloroquine resistant plasmodium falciparum malaria in east new britain, papua new guinea.	evidence of resistance ot chloroquine was found in 13 out of 14 children from the east new britain province of papua new guinea, with plasmodium falciparum malaria. infant amodiaquine also proved to be ineffective in preventing malaria in young children of one village in the province.	1980	7008425
[acute renal insufficiency in the course of plasmodium falciparum malaria (observations in 23 cases)].		1980	7009121
serum cholinesterase activity in patients with malaria infection.	serum cholinesterase activities were determined in 87 patients of both sexes with p. falciparum malaria in comparison to those of 80 blood donors. patients with acute p. falciparum malaria had significantly lower serum cholinesterase activity than those of the control group. after treatment, their serum cholinesterase levels returned to the normal level. serum albumin concentration also showed the same pattern and had a direct relationship to those of serum cholinesterase levels. these findings ...	1980	7013093
use of the in vitro microtechnique for the assessment of drug sensitivity of plasmodium falciparum in sennar, sudan.	in 1978, studies on the chloroquine sensitivity of plasmodium falciparum were carried out in the district of sennar, sudan. the results of the in vivo tests showed parasites resistant at the ri level only, but the mean clearance time of trophozoites from the blood was higher than for strains found in many other areas of tropical africa. the in vitro tests, using the microtechnique, indicated a lower sensitivity to chloroquine in the local p. falciparum isolates than in those of most other africa ...	1980	7028301
[drug resistance in malaria].		1980	7018421
plasmodium falciparum strains from papua new guinea: culture characteristics and drug sensitivity.	seven strains of plasmodium falciparum from papua new guinea have been established in continuous in vitro culture. samples with a high initial parasitaemia were more likely to form continuous lines, possibly due to the time required for transport of infected blood samples from papua new guinea to laboratories in australia. most but not all established lines were resistant to chloroquine and all were resistant to pyrimethamine, possibly reflecting the parasite strain characteristics in that regio ...	1980	7013092
malaria in southeast queensland in 1979.	the incidence of malaria in australia is increasing. with the emergence of strains of plasmodium falciparum resistant to chloroquine there are new lessons to be learned by laboratory workers in the species diagnosis; by clinicians in the clinical manifestations, the treatment of prophylaxis; and by members of the public about the dangers of malaria, and how it can be prevented. at the same time, old knowledge must not be forgotten. this paper reports some recent experience with a sizeable number ...	1980	7012564
morphology of babesia microti in human blood smears.	blood smears from 13 patients infected with babesia microti were reviewed to define the range of morphologic characteristics of the organism. organisms resembling rings of plasmodium falciparum were the predominant forms in all patients' blood smears. babesia microti and plasmodium spp. may be differentiated by the presence of pigment deposits in erythrocytes parasitized with mature stages of plasmodium.	1980	7188717
spectrum of haemolytic anaemias in punjab, north india.	between july 1974 and june 1978 the diagnosis of haemolytic anaemia was made in 267 patients. thalassaemia major was the leading cause (40-50%) every year except in 1977, when a sharp rise in drug induced haemolysis in g-6-pd deficient patients (61.3%) was encountered. this was possible due to an increase in incidence of vivax malaria and the use of anti-malarial drugs in that year. abnormal haemoglobins were noted in 9.7% of the total number of patients. hereditary spherocytosis, autoimmune hae ...	1980	7210170
human parasitoses in bali, indonesia.	a survey of intestinal and blood parasites of man was conducted on the island of bali, indonesia in 1974. a total of 270 stool specimens from three villages were examined. both ascaris lumbricoides and trichuris trichiura were found in more than 90% of those examined, and hookworm eggs in 59%. other intestinal helminth and protozoan parasites found were: enterobius vermicularis, entamoeba histolytica, entamoeba coli, endolimax nana, and iodamoeba bütschlii. taeniid proglottids, hymenolepis dimin ...	1980	7444572
short term cultivation of babesia species.	a method based on the candle jar technique was used for the cultivation of babesia bovis, b bigemina and b rodhaini in vitro. all three parasites multiplied within their host red blood cells and were well maintained for up to 96 h in culture. regular changes of medium and subculture at 24 h were essential for optimal parasite survival. a 40 per cent suspension of red blood cells was found to give better results than the 10 to 12 per cent suggested by jensen and trager for the cultivation of plas ...	1980	7455338
[malaria in the municipality of humaitá, state of amazonas. v - serological aspects of plasmodium falciparum and plasmodium berghei antigens].		1981	7046014
[malaria in the municipality of humaitá, state of amazonas. ix - incidence of blood group systems in inhabitants of the region and in patients].		1981	7046015
interactions of plasmodium falciparum infected erythrocytes with ligand-coated agarose beads.	the binding of normal and plasmodium falciparum-infected squirrel monkey erythrocytes to columns of ligand-coated agarose beads was compared. concanavalin a, ricin, soybean and peanut agglutinin-coated beads retain erythrocytes containing large developmental stages of the parasite preferentially to ring-containing erythrocytes or to normal erythrocytes. binding is inhibited by the sugar corresponding to the lectin's specificity. preferential binding does not occur with wheat germ or ulex europae ...	1981	7035941
[malaria in the municipality of humaitá, state of amazonas. xi - parasitological aspects].		1981	7046016
the in vivo sensitivity of plasmodium falciparum to chloroquine and to sulphadoxine-pyrimethamine combination in ibadan, nigeria.	fifty-eight nigerian children with plasmodium falciparum malaria were allocated randomly into two groups and treated with either chloroquine (25 mg/kg over three days) or fansidar (35 mg sulphadoxin (+ 1/20 pyrimethamine) per kg single dose)). they were observed for 28 days during which blood films were examined periodically for malaria parasites. asexual forms of p. falciparum, which were present in the blood films of all the patients in both groups before commencing treatment, disappeared rapi ...	1981	7036438
s-antigens and isozymes in strains of plasmodium falciparum.	evidence was found for the independent reassortment of parasite genes for s-antigens and isozymes. the lagos and palo alto strains of plasmodium falciparum had the same isozyme forms of pgd and ldh but different s-antigens. in contrast, the bw (gambia) and palo alto strains had different isozyme forms of gdh but shared some s-antigens. from artificial mixtures of parasites with different isozymes of gdh and different s-antigens, clones were derived in vitro which had a single isozyme type of gdh ...	1981	7036439
malaria blood cultures: a rapid fixation method to prevent sloughing of the thick film.		1981	7036441
the present status of malaria chemotherapy: mefloquine, a novel antimalarial.		1981	7050565
sulfadoxine-pyrimethamine resistant falciparum malaria in thai children.	sixty-eight children with uncomplicated falciparum malaria admitted to the hospital for tropical diseases in bangkok during april-december 1980 were randomly divided into 3 groups and given 3 regimens. group 1 of 27 cases were treated with a single dose of sulfadoxine 20 mg per kg body wt and pyrimethamine 1.0 mg per kg body wt. two cases (7.4%) were cured (s) while 4 cases (14.8%) showed ri failure, 17 cases (63.0%) rii failure and 4 cases (14.8) riii failure. in group 2, 18 cases were treated ...	1981	7043751
malaria antibody titres as measured by the indirect fluorescent antibody test in relation to parasitaemia and treatment.	two hundred and two sera from orang asli patients living in malarious areas were tested by the washed-cell, thick smear malaria ifa test. these patients were infected with p.vivax, p.falciparum and some with both parasites. antibodies to the homologous antigens were detected at titres of 1 : 16-1 : 4096 about 60 days after onset of treatment. eighteen months after cure antibody levels had fallen to lower levels of reactivity.	1981	7020094
plasmodium falciparum: synthesis of glycoprotein by cultured erythrocytic stages.		1981	7032961
simultaneous preparation of merozoites and concentrated schizonts of plasmodium falciparum.	a technique has been described for a simultaneous preparation of merozoites and concentrated schizonts. the merozoites were harvested after high speed centrifugation of either the supernatant of the low speed centrifugation of the plasmodium culture or the portion floated in physiogel. the concentrated schizonts were prepared by physiogel floatation technique followed by differential centrifugation in 50% percoll. an average of 56% of parasitized red cells were obtained, of which 64% were schizo ...	1981	7046074
chloroquine sensitivity of plasmodium falciparum in ibadan, nigeria: ii. correlation of in vitro with in vivo sensitivity.	plasmodium falciparum malaria was treated in 82 children with 25 mg/kg chloroquine orally over three days. they were observed for 28 days during which blood films were examined periodically for malaria parasites. asexual forms of p. falciparum, present in the blood films of all the patients before commencing treatment, disappeared rapidly and by the third day no parasites were seen in blood films from any of them. among the patients observed for more than three days, blood films remained negativ ...	1981	7036425
[current news in prophylaxis and malaria treatment (author's transl)].	the extension of chloroquines resistance of plasmodium falciparum, the emergence, still limited, of resistance to pyrimethamine-sulfadoxine, and even of multi-resistant strains, are facts all the more disturbing--especially for south east asia--that the number of antimalarials is limited. mefloquine appears to be a very promising drug; it might perhaps be desirable to be able to combine it with a new antimalarial, in collective prophylaxis, to prevent the selection of possibly resistant strains.	1981	7044583
[imported malaria: serological diagnostic and prevention of transfusional malaria (author's transl)].	some particular aspects, epidemiological, clinical, serological of imported malaria are studied about 137 cases seen in the department of tropical and parasitologic diseases of cochin hospital. the interest of studying several antigens, homologous and heterologous, in detection of fluorescent antibodies is discussed. in the case of any infesting plasmodium species, the use of homologous antigen gives very superior titers. systematic use of two antigens helps to detect possible antimalaria antibo ...	1981	7044584
inhibition of the in vitro growth of plasmodium falciparum by human polymorphonuclear neutrophil leucocytes.		1981	7039878
acute malaria in children in madang: endemicity, clinical presentation and treatment.	much of the present knowledge of the status of malaria in papua new guinea stems from a few studies carried out in the past. in the present situation there are many areas of uncertainty not least the effects of poor acceptance of residual spraying programmes and the emergence of chloroquine-resistant falciparum malaria. new anti-malarials are being developed, new methods of vector control may become useful and immunization may soon become possible. in order to assess the impact of these measures ...	1981	7039178
chloroquine-resistant plasmodium falciparum malaria in madang children.		1981	7039179
chloroquine resistant p. falciparum in chandrapur district of maharastra state.		1981	7045198
[a new case of plasmodium falciparum malaria resistance to chloroquine in africa (rwanda) (author's transl)].	report of a case of p. falciparum malaria observed in rwanda. relapse and positive blood thick smear has been observed 8 days after the beginning of the treatment of the previous fit of fever by chloroquine. authors recall the resistance criteria fixed by o.m.s. for antimalarial drugs and question wether drug-resistant malaria is not spreading in east-africa, considering some similar cases reported in the region.	1981	7040906
malaria parasite strain characterization, cryopreservation, and banking of isolates: a who memorandum.	there has been considerable progress in the biological characterization of malaria parasites in the past few years. physiological parameters such as host adaptation, virulence, exoerythrocytic development, in vitro growth of erythrocytic stages, and drug sensitivity are of particular importance to epidemiologists. advances in enzyme analysis, 2-dimensional protein electrophoresis, and nucleic acid analysis have produced several new techniques that can be applied to the malaria parasite. similarl ...	1981	7032732