Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| phosphomonoesterases of leishmania mexicana mexicana and other flagellates. | amastigotes and log-phase promastigotes of leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to ph and inhibitors. both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resistant acid phosphatase than promastigotes. a tartrate-sensitive acid phosphatase was secreted into the medium in large amounts during the growth in vitr ... | 1987 | 3037369 |
| nitroimidazole and oxygen derived radicals detected by electron spin resonance in hydrogenosomal and cytosolic fractions from trichomonas vaginalis. | hydrogenosome-enriched fractions from trichomonas vaginalis reduce a number of nitroimidazole derivatives to their respective electron spin resonance-detectable nitro-anion radicals. in the presence of of oxygen and 5,5-dimethyl-1-pyrroline-n-oxide (dmpo) a superoxide spin trapped adduct of dmpo was formed; the rate-determining step was the prior formation of the nitro-anion radical. oxygen-derived radicals were detected with cytosolic fractions from a metronidazole-resistant isolate (cdc-85) wh ... | 1987 | 3041214 |
| phenotypes and protein-epitope phenotypic variation among fresh isolates of trichomonas vaginalis. | fresh isolates of trichomonas vaginalis were examined for reactions to a panel of five monoclonal antibodies (mabs). four mabs (c20a3, dm126, dm116, and c55) were to distinct surface immunogens and one mab (l64) was to a cytoplasmic component. the fresh isolates were evaluated by indirect immunofluorescence (if), immunoblotting, and radioimmunoprecipitation. if assay with c20a3 mab gave isolates which were homogeneous nonstaining (negative [neg] phenotype) and isolates which were heterogeneous s ... | 1987 | 2437029 |
| [a comparative study of the incidence of gardnerella vaginalis in users of iud and oral contraceptives]. | leukorrhea constitutes one of the most frequent complaints by women visiting out-patient gynecological clinics. the most common etiological agents are gardnerella vaginalis, trichomonas vaginalis, neisseria gonorrhoea and candida albicans. some authors have been able to verify an increased presence of certain pathogenic germs in the vaginal flora for users of contraceptive methods, e.g., for users of iud, kivijarvi et al. demonstrated a significantly increased presence of gardnerella vaginalis ... | 1987 | 12282423 |
| [detection of igg antibodies with immunofluorescent antibody technique in human trichomoniasis] | the indirect fluorescent antibody(ifa) test was used to detect serum igg and igm antibodies to trichomonas vaginalis in 31 vaginal trichomoniasis, 7 candidiasis and in 20 non-infected healthy wonem with antigen prepared from axenic culture of trichomonas vaginalis isolated from vulvovaginitis patient. the results were as follows: in 31 vaginal trichomoniasis the positive reactions of igg antibody were 27 in the 1/8 dilution or higher and 4 in the 1/4 dilution whereas in healthy women the reactio ... | 1987 | 12886077 |
| anti-trichomonas vaginalis monoclonal antibodies inducing complement-dependent cytotoxicity. | hybridomas producing monoclonal antibodies (mabs) directed against trichomonas vaginalis (tv) have been produced in three fusions using mice immunized with live or killed tv. the elisa technique was used to test the binding activity of six out of the 48 mabs produced. it was found that acetone fixation enhanced the binding activity of the antibodies and revealed hidden antigenic determinants. thirty percent of the mabs obtained from splenocytes of mice immunized with live tv were of the igg3 sub ... | 1988 | 2448233 |
| diagnosis of trichomoniasis. comparison of conventional wet-mount examination with cytologic studies, cultures, and monoclonal antibody staining of direct specimens. | the accuracy of (1) conventional wet-mount examination, (2) papanicolaou-stained gynecologic smears, (3) a direct slide test using fluorescein-conjugated monoclonal antibodies against trichomonas vaginalis, and (4) two different culture media for the diagnosis of trichomoniasis in a high-risk population of 600 women was compared. use of feinberg-whittington or diamond's culture medium resulted in a diagnosis of 82 and 78 cases, respectively, and the combination of two cultures identified 88 infe ... | 1988 | 2448502 |
| diagnosis of chlamydia trachomatis infection--culture versus serology. | the diagnostic value of different laboratory methods in detecting chlamydia trachomatis infections in high risk groups was analysed. the efficiency of a direct specimen test was compared with serology (igg and igm elisa) and culture in l929 cells, stained either with fluorescein conjugated monoclonal antibodies or with iodine. patients (no. = 1041) with localized genital infections attending a std clinic, sexual contacts and patients with ascending infections from urological and gynecological cl ... | 1988 | 2451616 |
| antigenic heterogeneity in the 115,000 mr major surface antigen of trichomonas vaginalis. | the 115,000-molecular-weight antigen of trichomonas vaginalis was characterized using monoclonal antibodies developed to three different strains of t. vaginalis and one strain of tritrichomonas foetus. the antigen was found to be present on all strains or isolates of t. vaginalis examined and was demonstrated to be located on the external surface plasma membrane by agglutination assays and complement-mediated lysis assays. characteristics of the antigen were assessed with a proteolytic enzyme an ... | 1988 | 2456385 |
| nucleoside uptake by trichomonas vaginalis. | a rapid sampling technique was used to follow nucleoside uptake by trichomonas vaginalis. the results indicated that nucleoside uptake is biphasic with time. adenosine, guanosine, and uridine uptake is carrier mediated, transported substrate is rapidly metabolised to nucleotides. two separate carriers appear to exist, one which transports all nucleosides and a second which transports adenosine, guanosine and uridine. both carriers have more than one binding site for nucleosides. the first carrie ... | 1988 | 2457803 |
| evaluation of the laboratory diagnosis of vaginal trichomoniasis in khartoum. | a total of 403 vaginal discharge specimens were investigated for trichomonas vaginalis using fresh wet-mounts, culture and papanicolaou staining. by fresh wet-mounts, 58 specimens (14.4%) were found harbouring the parasite. on culture 67 isolates (16.7%) were identified. both wet-mount and cultural procedures detected 18.3% positive isolates. papanicolaou staining detected 40 positive cases (9.9%) from the patients investigated. two culture media were tried in this study to isolate t. vaginalis, ... | 1988 | 2462646 |
| quantitation of concanavalin a and wheat germ agglutinin binding by two strains of trichomonas vaginalis of differing pathogenicity using gold particle-conjugated lectins. | two strains of trichomonas vaginalis, which differed in their pathogenicity for both women and experimental animals hosts, were compared for the presence and number of concanavalin a (cona)- and wheat germ agglutinin (wga)-binding sites on their surface using gold lectin conjugates. both strains showed a high affinity for cona and wga and a similar pattern of gold particle distribution on the surface coat. the gold marker was distributed over the cytoplasmic membrane sparsely as single particles ... | 1988 | 2462736 |
| a simple flagella staining technique for trichomonas vaginalis. | 1988 | 2463454 | |
| [diagnostic-detecting value of selected methods used for evaluating the vaginal biocenosis in women]. | 1988 | 2463959 | |
| [pathogen spectrum of non-gonorrhea urethritis]. | by cultural investigations in 333 males with non-gonorrhoical urethritis (ngu) and 158 control probands it was demonstrated that chlamydia trachomatis is the main germ of this disease also in the gdr with a frequency of 40% of the cases. a chlamydia-negative ngu was caused in 15% by ureaplasma urealyticum in a number of greater than or equal to 10(4) germs/ml urine and in 5% by trichomonas vaginalis, respectively. candida albicans occurred significant more frequently in the patient group (21%) t ... | 1988 | 3043969 |
| new rapid latex agglutination test for diagnosing trichomonas vaginalis infection. | a newly developed latex agglutination test for trichomonas vaginalis infection was compared for sensitivity, specificity, efficiency, and positive and negative predictive values with microscopy, culture, and an enzyme linked immunosorbent assay (elisa) in the diagnosis of 395 women attending a genitourinary medicine clinic. t vaginalis infection was diagnosed in 42 (11%) women. the sensitivities of both the latex agglutination test and the elisa were 95% compared with 74% for microscopy and 76% ... | 1988 | 3045161 |
| analysis by immunofluorescence of alpha 1-antitrypsin levels on the surfaces of trichomonas vaginalis strains differing in virulence. | 1988 | 3045793 | |
| treatment failure in trichomonas vaginalis vaginitis. | 1988 | 3049301 | |
| alternating phenotypic expression of two classes of trichomonas vaginalis surface markers. | the antigenic heterogeneity of trichomonas vaginalis is due in part to the membrane disposition of immunogens (repertoire a) among some but not all isolates and among subpopulations of trichomonads of certain isolates. heterogeneous t. vaginalis isolates undergo phenotypic variation for the a repertoire of immunogens. the presence of immunogens on (a+ phenotype) or the absence of them from (a- phenotype) trichomonal surfaces clearly influences the virulence traits of the pathogenic human trichom ... | 1988 | 3055209 |
| [comparative study of the susceptibility of candida albicans and trichomonas vaginalis, on some products used as larvicides, trichomonacides and/or bactericides]. | 1988 | 3066257 | |
| localization of acetylated alpha-tubulin in tritrichomonas foetus and trichomonas vaginalis. | we used monoclonal antibodies specific for acetylated and nonacetylated alpha-tubulin to detect and to localize microtubules containing acetylated alpha-tubulin (stable microtubules) in the pathogenic protozoa tritrichomonas foetus and trichomonas vaginalis. sds-page analysis showed that tubulin is a major protein of both parasites, being enriched in cytoskeletal preparations of whole cells extracted with triton x-100. the monoclonal antibodies, which recognize all isoforms of alpha-tubulin (b-5 ... | 1988 | 3066505 |
| antiparasitic activity of nine pyrazole derivatives against trichomonas vaginalis, entamoeba invadens and plasmodium berghei. | nine nitropyrazole derivatives were prepared and tested against trichomonas vaginalis in vitro and in vivo, entamoeba invadens in vitro and plasmodium berghei in vivo. three of the compounds, 4-4-nitropyrazole, 1-methyl-4-nitropyrazole and 4,4'-dinitro-1,1'-methylenedipyrazole, have an activity similar to that of metronidazole (used as the reference compound) against t. vaginalis and e. invadens after 48 hours of incubation. all the compounds tested were inactive against p. berghei. | 1988 | 3074738 |
| prevalence of lower genital tract infections in pregnancy. | a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ... | 1988 | 3128883 |
| itraconazole: increased activity by chlorhexidine. | chlorhexidine increases the activity of itraconazole against candida isolates; itraconazole-chlorhexidine combinations show synergistic activity in culture media. the activity of itraconazole is discussed. | 1988 | 2839324 |
| frequency of cervico-vaginal infections (trichomonas vaginalis; chlamydia trachomatis -chl-; herpes simplex virus -hsv-; human papilloma virus -hpv-) in cervical intraepithelial neoplasia. | the authors have carried out a cyto-hystologic study on 533 cervical intraepithelial neoplasias (cin) devoted to identifying the association frequency between cervical dysplastic lesions and cervico-vaginal infections caused by trichomonas vaginalis, chlamydia trachomatis, herpes simplex virus and human papilloma virus. hpv lesions have revealed the pathology found more frequently in cin lesions (33.2%) compared to 0.19% in the normal population, while the other infections have not shown signifi ... | 1988 | 2839342 |
| [in vitro effect of nonoxynol-9 on herpes virus 1 and 2 and on other sexually transmitted micro-organisms]. | 1988 | 2854228 | |
| presence of laminin-binding proteins in trichomonads and their role in adhesion. | adhesion is regarded as an important feature in the pathogenesis of various microorganisms. ability to recognize extracellular matrix proteins, such as laminin or fibronectin, has been correlated with invasiveness. we report that laminin enhances the adhesion of the parasitic protozoa trichomonas vaginalis and tritrichomonas foetus to a polystyrene substrate and to the surface of epithelial cells (madin-darby canine kidney cell line) in vitro. the enhancement was higher for t. vaginalis than for ... | 1988 | 2973059 |
| evolutionary conservation of laminin-binding proteins. | 1. the virulence of pathogens and metastatic capacity of cancer cells seems to correlate with the ability to adhere to cells and/or to basement membrane components. a key feature of this mechanism is the expression of specific receptors for the basement membrane protein laminin. three different receptors have been already described in cells phylogenetically very distant, such as human white blood cells, trichomonas vaginalis and staphylococcus aureus, all recognizing laminin with the same range ... | 1988 | 2977953 |
| trichomonas vaginalis: characterization of its glutamate dehydrogenase. | an nadp-linked glutamate dehydrogenase (ec 1.4.1.4) was found in the soluble fraction of trichomonas vaginalis. its molecular weight was about 230,000 (gel filtration). the enzyme, partially purified by diafiltration and hydroxyapatite column chromatography, was heat stable (1 hr at 57 c). it catalyzed both the amination of alpha-ketoglutarate (mean km 0.6 mm) and the deamination of glutamate (mean km 1.2 mm) the optimum ph of the amination reaction was 6.7, and that of the deamination reaction ... | 1988 | 2901980 |
| sexually transmitted diseases in women with urethral syndrome. | frequency of micturition and dysuria were prominent symptoms in 135 (57%) of 237 women with urethral syndrome. ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis were the principal organisms associated with the urethral syndrome (38.41%, 28.14% and 11.11%, respectively). escherichia coli was cultured from four patients and herepes genitalis and neisseria gonorrhoea were isolated from five patients. infections with more than one organism were frequent. thirty-one of 135 patients ... | 1988 | 2903082 |
| [synthesis and antiparasitic activity of arylproprenones in the nitro-5-imidazole series]. | the synthesis of new arylpropenones derived from 1-methyl-5-nitro imidazole is described. the investigation of some antiprotozoal properties has shown that compounds with the groups 4-hydroxyphenyl, 4-chlorophenyl or thiophenyl have trichomonacidal activity similar to that of metronidazole. | 1988 | 2904887 |
| basic properties of tritrichomonas mobilensis hemagglutinin. | tritrichomonas mobilensis is a recently described enteric protozoon of squirrel monkeys. an earlier report identified one of the metabolic products of this organism as a lectinlike hemagglutinin. its further properties were determined in this study. culture supernatants of t. mobilensis fp4190 were concentrated by ultrafiltration through a membrane with 100,000-molecular-weight cutoff. high titers of agglutinin against human erythrocytes were obtained. incubation at 70 degrees c for 15 min resul ... | 1988 | 3170709 |
| the interaction of trichomonas vaginalis and tritrichomonas foetus with epithelial cells in vitro. | the madin-darby canine kidney epithelial cell line (mdck) was used as a model for trichomonad-host cell interaction. two laboratory strains of the human parasite trichomonas vaginalis and the cattle's parasite tritrichomonas foetus or their supernatants from axenic cultures were allowed to interact with confluent epithelial cultures. the interaction process studied by transmission and scanning electron microscopy revealed that both parasites adhere to monolayers through flagella, cell body and p ... | 1988 | 3180257 |
| effects of antimicrobial therapy on sperm-mucus interaction. | sperm-mucus interaction under in-vitro or in-vivo conditions might be affected by microorganisms colonizing the reproductive tract. in order to study the influence of antimicrobial therapy, an extensive microbial screening was performed including chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, a broad spectrum of potentially pathogenic aerobic and anaerobic bacteria, trichomonas vaginalis, herpes simplex virus and yeasts. one-hundred-and-six couples with ... | 1988 | 3182976 |
| prostitution in sheffield: differences between prostitutes. | this study to assess differences between street walking prostitutes and sauna girls who attended this clinic in 1986 and 1987 showed that fewer street walkers used barrier methods for disease prevention with clients or accepted tests for antibody to human immunodeficiency virus (hiv) when offered and more street walkers practised their profession in both sheffield and london. they therefore represent a potential pathway for the heterosexual spread of hiv to the region. trichomonas vaginalis was ... | 1988 | 3224974 |
| giardia-bile salt interactions in vitro and in vivo. | giardia lamblia predominantly colonizes the proximal small intestine where bile is plentiful. we have investigated interactions between bile and this parasite by (i) examining the specificity of the stimulatory effect of bile on parasite growth in vitro, (ii) studying the possible association between giardiasis and bile salt deconjugation in vivo, and (iii) quantifying bile salt uptake by giardia and relating this to uptake by other microorganisms. our findings indicate that the growth promoting ... | 1988 | 3232181 |
| giardia duodenalis: enhanced growth in cell culture. | growth of giardia duodenalis in broth and in animals has been studied in considerable detail. in contrast, the kinetics of growth in cell culture have been little evaluated. in this study, in vitro growth of g. duodenalis was evaluated in cell culture, primarily using mouse mccoy cells in vials. the media used were giardia broth (tyi-g), trichomonas vaginalis broth (tyi-t), and standard cell culture media (cmga) alone and in combination (2 parts by volume cmga to one part of tyi broth). addition ... | 1988 | 3232182 |
| multiple populations of double-stranded rna in two virus-harbouring strains of trichomonas vaginalis. | the existence of six dsrna segments of trichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsrna serving as a standard. the m's were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. cell homogenate fractionation showed that all dsrna se ... | 1988 | 3240907 |
| [cyto-microbiologic study of cervico-vaginal specimens, associated with a serologic study to detect sexually transmissible diseases in madagascan prostitutes]. | a microbiological survey of cervical and vaginal smears associated with a serological survey of sexual diseases was carried out on 298 prostitutes at the four principal malagasy sea ports. this survey leads us to these conclusions: the great frequency of specific inflammations (72.1%) with trichomonas vaginalis in the first place (39.2%). the serological tests proved a high rate of positive treponemical serologies (25.2%), whose the half of the cases are evolutive forms. nevertheless prevalence ... | 1988 | 3242427 |
| identification and properties of trichomonas vaginalis proteins involved in cytadherence. | trichomonas vaginalis nyh286 surface proteins which are candidates for mediating parasite cytadherence (adhesins) were identified. at least four trichomonad protein ligands ranging in relative molecular mass from 65 to less than or equal to 21 kilodaltons were found to selectively bind to chemically stabilized hela cells. the proteins were present on the surfaces of 10 different isolates of t. vaginalis examined; however, the nonpathogenic trichomonad t. tenax did not possess similar hela cell-b ... | 1988 | 3257206 |
| the diagnosis of vaginal trichomoniasis. | 1988 | 3257533 | |
| the purification and properties of two soluble reduced nicotinamide: acceptor oxidoreductases from trichomonas vaginalis. | the occurrence of soluble reduced nicotinamide nucleotide:acceptor oxidoreductases has been reported in a number of strains of the oxygen-tolerant anaerobe trichomonas vaginalis and other trichomonad species. the quantitatively more important enzyme in most strains of t. vaginalis is an nadh oxidase which produces water from the reduction of oxygen. this enzyme has been purified by a combination of gel filtration, chromatofocusing, cibacron blue chromatography and high pressure gel permeation ch ... | 1988 | 3257811 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | the aims of the present study were to count trichomonas vaginalis organisms recovered from the vaginas of patients with trichomoniasis, and to obtain data concerning changes in sizes of trichomonal populations during the menstrual cycle. in about 80% of symptomatic as well as symptomless patients, more than 1 x 10(5) parasites per ml could be obtained from vaginal washes. during menstruation, however, the number of trichomonads decreased appreciably, with subsequent increases within three to six ... | 1988 | 3257936 |
| in vitro susceptibility of trichomonas vaginalis to 50 antimicrobial agents. | we determined the susceptibilities of five strains of trichomonas vaginalis, one of which was metronidazole resistant, to 50 antimicrobial agents. for the metronidazole-susceptible strains, the most active agents were metronidazole, tinidazole, mebendazole, furazolidone, and anisomycin. against the resistant strain mebendazole, furazolidone, and anisomycin were the most active. antifungal agents, beta-lactams, macrolides, aminoglycosides, and folic acid antagonists were ineffective against all s ... | 1988 | 3258142 |
| use of a crude extract or a purified antigen of trichomonas vaginalis for the detection of secretory antibodies by elisa. | a crude aqueous extract (t2) or a gel-filtration purified antigen of t. vaginalis was utilized to detect secretory antibodies in cervico-vaginal secretions by elisa. the test was developed under optimum conditions using a rabbit anti-trichomonas serum. conditions established for monitoring antibodies to trichomonas in immunized rabbits were equally effective for human secretions. the crude extract was capable of assessing secretory antibodies in 45% of women with acute trichomoniasis and 43% of ... | 1988 | 3258398 |
| mouse spleen cell responses to trichomonal antigens in experimental trichomonas vaginalis infection. | subcutaneous inoculation of live t. vaginalis into mice caused splenomegaly, particularly when using strains of parasites with low pathogenicity. the proliferative responses of spleen cells from uninfected mice, as measured by [3h] tdr uptake, showed that trichomonal antigens, whether from strains with high or low pathogenicity, have no mitogenic activity. spleen cells from mice infected with trichomonads of low pathogenicity showed a proliferative response to trichomonal antigens that was maxim ... | 1988 | 3258637 |
| in vitro susceptibility of trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. | we have identified trichomonas vaginalis strains resistant in vitro to metronidazole, especially under aerobic conditions. since little is known about the relationship of treatment outcome to metronidazole susceptibility of t. vaginalis, we studied the aerobic and anaerobic susceptibility to metronidazole of 310 clinical isolates of t. vaginalis. of 199 patients with known outcomes after metronidazole treatment for vaginal trichomoniasis, the geometric mean minimal lethal concentration (mlc) und ... | 1988 | 3258675 |
| determination of cytopathogenic effects of trichomonas vaginalis on cultured cells by assaying 51cr release. | by use of an assay for 51cr release, it was attempted to determine the cytopathogenic effects of trichomonas vaginalis on mammalian cultured cells. 51cr was released from na2(51)cro4-labeled hela, l-929, fl, vero and llc-mk 2 cells. hela cells were most sensitive to the damage, followed by l-929, fl, vero and llc-mk 2 cells. the effect depended upon the number of parasite and the incubation period. the maximum 51cr release from the labeled cells was observed at 37 degrees c and ph 6.0. these res ... | 1988 | 3259767 |
| flagella-bearing bare trichomonas vaginalis nuclei in papanicolaou smears. | 1988 | 3259922 | |
| [anthelmintic agents. 5. long-chain and hydroheterocyclic-substituted chloro-diallylamino-1,3,5-triazines]. | 1988 | 3260475 | |
| differential susceptibility of fresh trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus. | the ability of complement in human menstrual blood and cervical mucus to kill trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. only serum and menstrual blood with haemolytic activity produced total cytolysis of t vaginalis. the cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (edta) ... | 1988 | 3261708 |
| specific parasitism of purified vaginal epithelial cells by trichomonas vaginalis. | human vaginal epithelial cells (vecs) from vaginal swabs obtained from normal women or from patients with trichomoniasis were purified, and vec parasitism by trichomonas vaginalis was examined. trichomonads bound equally well to live or dead vecs, and up to 20% of vecs were parasitized. trichomonal cytadherence of human vecs was time, temperature, and ph dependent. saturation binding levels of live trichomonads to vecs gave approximately 2 organisms adherent to parasitized vec. no differences in ... | 1988 | 3262088 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | 1988 | 3262565 | |
| [observation on trichomonas vaginalis by scanning electron microscopy]. | 1988 | 3263220 | |
| monoclonal-antibody-based enzyme-linked immunosorbent assay for trichomonas vaginalis. | trichomonas vaginalis is estimated to infect 4 million women per year in the united states. the diagnosis of trichomoniasis is predominantly achieved by direct microscopic examination of vaginal exudates. this subjective diagnostic procedure is reported to be 75% sensitive under ideal circumstances. we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of t. vaginalis directly from vaginal exudates. the elisa employs a monoclonal antibody specific for a 65-kilodalton s ... | 1988 | 3263386 |
| demonstration of concanavalin a- and wheat germ agglutinin-binding sites on the trichomonas vaginalis surface coat using lectin-gold particle conjugates. | 1988 | 3263459 | |
| [morphology and biological activity of trichomonas vaginalis grown on various nutrient media]. | 1988 | 3264099 | |
| [ultrastructural analysis of the interrelation of the causative agents of urogenital infections with the cells of the macro-organism in syphilis patients]. | 1988 | 3264100 | |
| effects of antimicrobial agents on growth and chemotaxis of trichomonas vaginalis. | the motility of viable trichomonas vaginalis organisms is readily demonstrable in a clinical wet mount or cultured specimens. we attempted to determine whether migration is a dynamic process such that the organisms move to avoid exposure to toxic antimicrobial agents. with the use of axenic cultures of t. vaginalis that were radiolabeled and assayed for chemotaxis in plastic multiwelled plates with a membrane filter inserted to trap organisms, the response of clinical isolates to various antimic ... | 1988 | 3264130 |
| [verification of the value of the elisa test in the diagnosis of urogenital trichomoniasis]. | 1988 | 3264747 | |
| [use of the elisa method in the determination of antibodies to trichomonas vaginalis]. | 1988 | 3264749 | |
| [complement-binding antibodies in women simultaneously infected with various species of trichomonas]. | 1988 | 3265240 | |
| [intradermal test with trichomonas vaginalis allergen in patients with urogenital trichomonas infection]. | 1988 | 3265243 | |
| the effect of hormones on trichomonas vaginalis. | the hormonal milieu can alter susceptibility to infection. the effect of hormones on trichomonas vaginalis was studied utilizing axenically cultured clinical isolates. oestrogens, in physiological concentrations, decreased the growth of the organisms and their attachment to mammalian cells in vitro, and acted as a chemorepellent. the specificity of these effects was verified by their being blocked with anti-oestrogens, by the dose- and time-dependency of the responses, and by the lack of effect ... | 1988 | 3265429 |
| [a dried nutrient medium made from the fermented hydrolysates of a microorganism biomass for isolating trichomonas vaginalis]. | 1988 | 3265559 | |
| human amnion membrane as a model for studying the host-parasite relationship in trichomoniasis. | 1988 | 3265702 | |
| isoenzyme patterns of isolates of trichomonas vaginalis from vancouver. | isoenzyme patterns of 63 isolates of trichomonas vaginalis obtained in vancouver were evaluated by use of thin-layer starch-gel electrophoresis. we attempted to use eight enzymes, but only four gave reproducible and interpretable results. there were four patterns with malic enzyme, two with malate dehydrogenase, one with hexokinase, and four with lactate dehydrogenase. the isoenzyme patterns of the 63 isolates were classified into 15 groups, but 49 (78%) fell into five groups and 14 (22%) fell i ... | 1988 | 3265809 |
| [sensitivity of trichomonas vaginalis to desiccation]. | 1988 | 3266051 | |
| trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor. | this study determined the effects of trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic t. vaginalis cultures on human sperm motility and viability. spent medium (ph 7.0) caused complete cessation of sperm motility after 15 minutes incubation. trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three per ... | 1988 | 3266454 |
| effect of metronidazole on the cell surface charge of trichomonas vaginalis and tritrichomonas foetus. | 1. the effect of metronidazole, a drug used in the treatment of trichomoniasis, on the surface charge of both trichomonas vaginalis and tritichomonas foetus was analyzed by cell electrophoresis. 2. incubation of the parasites under anaerobic conditions for 2 or 120 min in the presence of 2.0 micrograms/ml metronidazole, a concentration which inhibits cell growth by about 50%, led to a marked decrease in the net surface charge of the parasites. 3. the metronidazole analogue, 1-hydroxyethyl-2-meth ... | 1988 | 3266844 |
| [trichomonas vaginalis, epithelial cells, spermatozoa, and immunologic reactions. how and why an infection persists unchanged despite knowing an adequate drug therapy]. | 1988 | 3267183 | |
| sexually transmitted diseases and sexual behavior in urban adolescent females attending a family planning clinic. | specimens from the lower genital tract of 102 sexually active urban adolescent females were tested for the common sexually transmitted diseases (stds). the results were correlated with concomitant clinical, demographic, and historic data. forty-one percent (42/102) were infected with one or more of the following organisms: chlamydia trachomatis (26/102), trichomonas vaginalis (13/102), neisseria gonorrhoeae (10/102), yeast (6/102), condyloma (5/102), and herpes simplex virus (1/44). mixed infect ... | 1988 | 3335474 |
| influence of gender in pathogenesis of trichomoniasis in congenitally athymic (nude) mice. | evaluation of abscesses appearing in male and female euthymic and athymic (nude) balb/c mice after subcutaneous injection of trichomonas vaginalis in the dorsal region showed that females were more susceptible than males. female euthymic mice, however, were more susceptible than male athymic mice, and splenectomised athymic males were more susceptible than non-splenectomised athymic males. f1 female athymic mice were the most susceptible, as their abscesses reached a peak size five days earlier ... | 1988 | 3346023 |
| [trichomonas vaginalis infection. frequency and diagnosis in women]. | 1988 | 3353949 | |
| association of coexisting vaginal infections and multiple abusers in female children with genital warts. | all 11 girls less than 12 years of age presenting to the pediatric clinic between 1980 and 1986 with genital warts were evaluated by the child protection team for possible sexual abuse. nine of the 11 girls had condylomata acuminata, and two had verruca vulgaris. ten of the 11 had historical and/or physical evidence other than the warts that confirmed sexual abuse. six girls had coexisting vaginal infections such as bacterial vaginosis and infections due to various pathogens including neisseria ... | 1988 | 3358240 |
| treatment failure in trichomonas vaginalis infections in females. i. concentrations of metronidazole in plasma and vaginal content during normal and high dosage. | concurrent estimation by high pressure liquid chromatography (hplc) of the concentrations of metronidazole in plasma and vaginal content in 12 patients with infections due to trichomonas vaginalis who did not respond to normal and high-dose treatment has shown that the two levels are closely related to each other and to the dose. individual idiosyncrasies in absorption of drug from the gut lumen or in its transfer into the vaginal content are unlikely to be the cause of treatment failure. | 1988 | 3360695 |
| trichomonal vaginitis: evaluation of serological tests and identification of immunoreactive surface peptides. | an indirect haemagglutination assay (iha) with polysaccharide and protein antigens of trichomonas vaginalis and an enzyme linked immunosorbent assay (elisa) were used to test for antibodies against t vaginalis in 58 women with trichonomal vaginitis and 48 with non-specific vaginitis. eleven antibody positive sera were used in a radioimmunoprecipitation assay (ripa) to identify surface peptides that elicited antibody responses in infected women. the serological tests were less sensitive than biol ... | 1988 | 3384427 |
| [metronidazole-resistant trichomoniasis and successful therapy following high dosage]. | a patient is reported who suffered for several months from a trichomonas vaginalis infection that was resistant to the usual low-dose treatment with 5-nitro-imidazole derivatives. following various ineffective therapeutic trials, the agent was isolated in order to determine its sensitivity to 5-nitro-imidazole. the resistance of the isolate to metronidazole was confirmed in vitro and in an animal experiment; the patient was therefore treated with high daily doses of metronidazole, 3 x 750 mg ora ... | 1988 | 3384665 |
| treatment failure in trichomonas vaginalis infections in females. ii. in-vitro estimation of the sensitivity of the organism to metronidazole. | we have developed a standard procedure to measure the susceptibility of trichomonas vaginalis to metronidazole in vitro, by controlling the state of oxygenation of the test cultures, the size of the inocula in terms of infectivity, and providing multiple tests at each drug concentration. estimates were made of the in-vitro sensitivity to metronidazole of t. vaginalis isolated from 11 randomly selected patients, all, except for three defaulters, known to have been treated successfully, and from s ... | 1988 | 3391860 |
| pathogenicity of tritrichomonas mobilensis: subcutaneous inoculation in mice. | the standard "subcutaneous mouse assay" was used to investigate the inherent pathogenicity of tritrichomonas mobilensis, an intestinal parasite of squirrel monkeys. c57b1/6 mice given subcutaneous bilateral inocula of t. mobilensis died by day 4 postinoculation with lesions too small to be measured. control mice similarly inoculated with pathogenic and nonpathogenic strains of trichomonas vaginalis survived the challenge and produced lesions on day 6 with mean volumes in agreement with previous ... | 1988 | 3418457 |
| genetic differentiation and biochemical polymorphism among trichomonads. | isoenzyme electrophoresis was used to study levels of genetic differentiation among strains and clones of trichomonas gallinae, trichomonas vaginalis, tritrichomonas foetus, tetratrichomonas gallinarum, and pentatrichomonas hominis. strain variation was found within t. gallinae, t. vaginalis, and t. foetus, however, levels of enzyme polymorphism were greater in t. gallinae than in t. vaginalis or t. foetus. isoenzyme genotypes were not a stable property of t. gallinae clones cultivated in vitro. ... | 1988 | 3418458 |
| managing trichomonal vaginitis refractory to conventional treatment with metronidazole. | three patients with vulvovaginitis caused by trichomonas vaginalis, which was refractory to conventional treatment with metronidazole are described. the t vaginalis strain isolated from one patient was resistant to metronidazole (minimum inhibitory concentration (mic) more than 100 mg/l) under aerobic conditions, although under anaerobic conditions it was as susceptible as a normal reference strain. the effect of the concomitant use of other medication and the influence of other vaginal pathogen ... | 1988 | 3278971 |
| establishing a correct diagnosis of vulvovaginal infection. | vulvovaginitis is a common problem and a prime reason for physician consultation. the organism most frequently responsible for vulvovaginal infection is candida albicans, and in a large majority of cases identification of the causative organism and diagnosis are easy to establish. recurrent or persistent infection, however, presents a perplexing diagnostic problem that usually can be solved with the use of appropriate supportive laboratory tests and awareness of the distinguishing features of th ... | 1988 | 3284366 |
| practical problems of diagnosing trichomoniasis in women. | analysis of eight groups of data collected at varying intervals during a period of seven years showed fluctuations in the sensitivity of tests to diagnose trichomoniasis in women. the best results were obtained from fresh, correctly prepared diamond's medium, feinberg-whittington's medium, and modified squires and mcfadzean's medium (which showed 82% to 94% relative sensitivity). poor results were identified consistently in commercially prepared bushby medium from one source (40% relative sensit ... | 1988 | 3290090 |
| does lactobacillus vaccine for trichomoniasis, solco trichovac, induce antibody reactive with trichomonas vaginalis? | solco trichovac is a vaccine of lactobacillus acidophilus developed for treating trichomoniasis. the efficacy of the vaccine is reportedly due to cross reacting antibody being produced in people immunised with the lactobacillus bacteria. several techniques, including enzyme linked immunosorbent, indirect immunofluorescence, immunoblot, and radioimmunoprecipitation assays were used to assess the extent of antigenic cross reactivity between lactobacillus acidophilus and trichomonas vaginalis. data ... | 1988 | 3290091 |
| the release of hydrolases from trichomonas vaginalis and tritrichomonas foetus. | trichomonas vaginalis and tritrichomonas foetus were found to release large amounts of beta-n-acetylglucosaminidase (ec 3.2.1.30), alpha-mannosidase (ec 3.2.1.24), beta-glucosidase (ec 3.2.1.21), acid phosphatase (ec 3.1.3.2) and proteinases during axenic growth in vitro. the enzymes were released continually throughout the growth phase, with the extracellular activity being of the same order as that within the cells. there was differential release of proteinases from trichomonas vaginalis. the ... | 1988 | 3140008 |
| gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra. | in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ... | 1988 | 3140532 |
| comparison of four methods to detect trichomonas vaginalis. | four methods for the detection of trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, kupferberg liquid medium, hirsch charcoal agar, and the papanicolaou smear. hirsch diphasic slants and kupferberg medium did not significantly differ in sensitivity from direct examination of wet mounts. the papanicolaou smear identification of trichomonads was found to be the least sensitive method evaluated. | 1988 | 3141470 |
| effect of dl-alpha-difluoromethylornithine on polyamine synthesis and interconversion in trichomonas vaginalis grown in a semi-defined medium. | growth of trichomonas vaginalis in a semi-defined medium was inhibited by 5 mm dl-alpha-difluoromethylornithine (dfmo). using high pressure liquid chromatography (hplc) analysis, putrescine and cadaverine levels were found to be 90 and 100% reduced, respectively after 120 h exposure, whilst spermidine and spermine levels were unchanged. putrescine (40 microm) and cadaverine (6 microm) were detected in the spent media from control cultures. neither of these diamines was detected in spent media fr ... | 1988 | 3141809 |
| microbiological profile of the cervix in 1,000 sexually active women. | one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ... | 1988 | 3148302 |
| some aspects of the diagnosis of specific vaginal infections in the rotterdam std clinic population. | we studied specific vaginal infections in 351 consecutive women who visited the rotterdam std clinic. prostitutes less often had vaginal or vulvar complaints than non-prostitutes (fisher test, p less than 0.01), but more often had abnormal discharge (fisher test, p less than 0.001). normal secretions were found in 52% of the women. clue cell-positive discharge (ccpd) was by far the most prevalent vaginal disease entity (32%). the wet mount showed pseudo-hyphae in 14 women (4%) and trichomonas va ... | 1988 | 3134262 |
| lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology. | we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ... | 1988 | 3135608 |
| effect of a killer toxin of yeast on eucaryotic systems. | the saccharomyces cerevisiae killer toxin kt 28, which inhibits sensitive yeasts, was shown to have no effect on several pathogenic fungi or on the protozoan trichomonas vaginalis. at concentrations of about 0.1 mg/ml, a partial inhibition of the skin pathogenic fungi trichophyton rubrum and microsporum canis was observed at ph 6.5. no pharmacological activity was detected in various tests with several animal organs. | 1988 | 16347605 |
| polyamine biosynthesis and inhibition in trichomonas vaginalis. | polyomines - particularly putrescine, spermidine and spermine - are ubiquitous components of eukaryote and most prokaryote cells, and are essential for optimal cell proliferation. but since routes of polyamine synthesis may differ, for example between parasites and their hosts, selective inhibition of polyamine metabolism offers an attractive target for chemotherapy - as already shown with the success of difluoromethylomithine (dfmo) as an inhibitor of polyamine synthesis in african trypanosomes ... | 1988 | 15463030 |
| trichomonas infections in men. | trichomonas vaginalis, a common pathogen in the female genital tract, produces a characteristic clinical picture in women. less well recognized are the manifestations of trichomonas infestations of the male genital tract, which include urethritis and chronic prostatitis. multiple-glass urinalysis and selective use of trichomonas cultures may improve recognition of this organism in the family practice setting. | 1989 | 2916396 |
| detection of microorganisms in clinical specimens using slides prestained with acridine orange (aos). | slides prestained with acridine orange (aos) were used for direct examination of microorganisms in clinical specimens. the method is simpler than a commonly used acridine orange stain (ao) and has the advantage of being a supravital stain. results indicate that the aos method is sensitive, and allows rapid detection of microorganisms, even in the specimens containing few microorganisms, e.g. cerebrospinal fluid. moreover aos may be the choice method for detection of trichomonas vaginalis. | 1989 | 2469942 |
| trichomonas vaginalis--identification by rapid bpt-drăgan staining. | the blue-polychrome-tannin rapid staining (bpt-drăgan, 1968) applied on fresh and dried vaginal smears makes possible the identification of different forms of trichomonas vaginalis (tv) parasite. the nucleus is coloured in blue-violet and the cytoplasm in various degrees of blue-green, violet or bichromatical, with blue and violet zones at the cell periphery. in the cytoplasm, the staining allows the identification of many granules and vacuoles. all the stainable substrates have an acid characte ... | 1989 | 2478878 |
| comparison of direct fluorescent antibody, acridine orange, wet mount, and culture for detection of trichomonas vaginalis in women attending a public sexually transmitted diseases clinic. | to define the performance characteristics of two newer tests for trichomonas vaginalis (tv), the authors compared direct fluorescent antibody (dfa) (mixed monoclonal antibody, integrated diagnostics, inc, berkeley, ca) and acridine orange (ao) tests to standard wet mount (wm) preparations and culture (modified diamond medium) of vaginal wash specimens in consecutively examined women presenting to a public sexually transmitted diseases (std) clinic. cultures for neisseria gonorrhoeae (gc), chlamy ... | 1989 | 2479110 |
| [antimicrobial characteristics of a tincture of dequalinium chloride]. | dequalinium is a quaternary ammonium salt. from about 30 years dequalinium (d.c.) was used in the treatment of the initial respiratory organs infections. it can be given orally and topically yet it cannot be given systemically cause its probable systemic toxicity. d.c. has a wide range of antimicrobial activity that is extended to gram positive, gram negative bacteria, protozoa and yeast. its mechanism of action is directed to the cytoplasmatic membrane where d.c. caused its damage and consequen ... | 1989 | 2483904 |
| [antimicrobial activity exerted by sodium dichloroisocyanurate]. | sodium dichloroisocyanurate is a chlorinated cleaner. it was used for swimming pool sanitation and for the sterilisation of linen. not recently ago sodium dichloroisocyanurate has substituted hypochlorite for the sterilisation of infant feeding bottles and teats. sodium dichloroisocyanurate is soluble in water; this condition causes the hydrolysis of sodium dichloroisocyanurate in hypochlorous acid, that is the active agent, isocyanurate and isocyanurate chlorine. these compounds form a chlorine ... | 1989 | 2484478 |