Publications

synthesis and bioactivities of phenazine-1-carboxylic acid derivatives based on the modification of pca carboxyl group.	phenazine-1-carboxylic acid (pca) as a natural product widely exists in microbial metabolites of pseudomonads and streptomycetes and has been registered for the fungicide against rice sheath blight in china. to find higher fungicidal activities compounds and study the effects on fungicidal activities after changing the carboxyl group of pca, we synthesized a series of pca derivatives by modifying the carboxyl group of pca and their structures were confirmed by (1)h nmr and hrms. most compounds e ...	2017	28320617
fitness of three chemotypes of fusarium graminearum species complex in major winter wheat-producing areas of china.	in china, fusarium head blight is caused mainly by the fusarium graminearum species complex (fgsc), which produces trichothecene toxins. the fgsc is divided into three chemotypes: 3-acetyldeoxynivalenol (3-adon), 15-acetyldeoxynivalenol (15-adon), and nivalenol (niv). in order to predict the geographical changes in the distribution of these chemotype populations in major winter wheat-producing areas in china, the biological characteristics of twenty randomly selected isolates from each of the th ...	2017	28306726
the complete genome sequence of a double-stranded rna mycovirus from fusarium graminearum strain hn1.	the complete nucleotide sequence of a double-stranded rna (dsrna) mycovirus, fusarium graminearum dsrna virus 5 (fgv5), was identified and characterized. the fgv5 genome comprises two dsrna genome segments of 2030 bp and 1740 bp. fgv5 dsrna1 contains a single open reading frame (orf1), which is predicted to encode a protein of 613 amino acids (aa) with a molecular mass of 70.4 kda and has a conserved rna-dependent rna polymerase (rdrp) motif. fgv5 dsrna2 is predicted to contain two discontinuous ...	2017	28299481
functional characterization of cytochrome p450 monooxygenases in the cereal head blight fungus fusarium graminearum.	fusarium graminearum is a prominent plant pathogenic fungus causing fusarium head blight in major cereal crops worldwide. to understand the molecular mechanisms underlying fungal development and virulence, large collections of f. graminearum mutants have been constructed. cytochrome p450 monooxygenases (p450s) are widely distributed in organisms and are involved in a diverse array of molecular/metabolic processes; however, no systematic functional analysis of p450s has been attempted in filament ...	2017	28296081
structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis.	compartmentalization of metabolic pathways to particular organelles is a hallmark of eukaryotic cells. knowledge of the development of organelles and attendant pathways under different metabolic states has been advanced by live cell imaging and organelle specific analysis. nevertheless, relatively few studies have addressed the cellular localization of pathways for synthesis of fungal secondary metabolites, despite their importance as bioactive compounds with significance to medicine and agricul ...	2017	28287158
genetic mapping and haplotype analysis of a locus for quantitative resistance to fusarium graminearum in soybean accession pi 567516c.	a major novel quantitative disease resistance locus, qrfg_gm06, for fusarium graminearum was genetically mapped to chromosome 6. genomic-assisted haplotype analysis within this region identified three putative candidate genes. fusarium graminearum causes seed, root rot, and seedling damping-off in soybean which contributes to reduced stands and yield. a cultivar magellan and pi 567516c were identified with low and high levels of partial resistance to f. graminearum, respectively. quantitative di ...	2017	28275816
exploiting sp2-hybridization in the development of potent 1,5-a-l-arabinanase inhibitors.	the synthesis of potent inhibitors of gh93 arabinanases as well as a synthesis of a chromogenic substrate to measure gh93 arabinanase activity is described. an insight into the reasons behind the potency of the inhibitors was gained through x-ray crystallographic analysis using the arabinanase arb93a from fusarium graminearum. these compounds lay a foundation for future inhibitor development as well as for the use of the chromogenic substrate in biochemical studies of gh93 arabinanases.	2017	28266777
characterization of the two-speed subgenomes of fusarium graminearum reveals the fast-speed subgenome specialized for adaption and infection.	fusarium head blight, caused by fusarium graminearum, is one of the most severe diseases on wheat and barley worldwide. although the genomic data of several strains were published, the intragenomic variation of f. graminearum was not well characterized. here, we sequenced three chinese strains and conducted genome-wide comparisons. our data revealed that all the sequenced strains were distinct from each other and over 350 genes were functionally lost in each of them. variants of each strain were ...	2017	28261228
landscape of genomic diversity and host adaptation in fusarium graminearum.	fusarium graminearum is one of the main causal agents of the fusarium head blight, a worldwide disease affecting cereal cultures, whose presence can lead to contaminated grains with chemically stable and harmful mycotoxins. resistant cultivars and fungicides are frequently used to control this pathogen, and several observations suggest an adaptation of f. graminearum that raises concerns regarding the future of current plant disease management strategies. to understand the genetic basis as well ...	2017	28231761
a gin4-like protein kinase gil1 involvement in hyphal growth, asexual development, and pathogenesis in fusarium graminearum.	fusarium graminearum is the main causal agent of fusarium head blight (fhb) on wheat and barley. in a previous study, a gin4-like protein kinase gene, gil1, was found to be important for plant infection and sexual reproduction. in this study we further characterized the functions of gil1 kinase in different developmental processes. the δgil1 mutants were reduced in growth, conidiation, and virulence, and formed whitish and compact colonies. although phialide formation was rarely observed in the ...	2017	28212314
optimization for the production of deoxynivalenoland zearalenone by fusarium graminearum usingresponse surface methodology.	fusarium mycotoxins deoxynivalenol (don) and zearalenone (zen) are the mostcommon contaminants in cereals worldwide, causing a wide range of adverse health effects onanimals and humans. many environmental factors can affect the production of these mycotoxins.here, we have used response surface methodology (rsm) to optimize the fusarium graminearumstrain 29 culture conditions for maximal toxin production. three factors, medium ph, incubationtemperature and time, were optimized using a box-behnken ...	2017	28208576
tawrky70 transcription factor in wheat qtl-2dl regulates downstream metabolite biosynthetic genes to resist fusarium graminearum infection spread within spike.	a semi-comprehensive metabolomics was used to identify the candidate metabolites and genes to decipher mechanisms of resistance in wheat near-isogenic lines (nils) containing qtl-2dl against fusarium graminearum (fg). metabolites, with high fold-change in abundance, belonging to hydroxycinnamic acid amides (hcaas): such as coumaroylagmatine, coumaroylputrescine and fatty acids: phosphatidic acids (pas) were identified as resistance related induced (rri) metabolites in rachis of resistant nil (ni ...	2017	28198421
deoxynivalenol exposure in norway, risk assessments for different human age groups.	deoxynivalenol (don) is the most common mycotoxin in norwegian cereals, and don is detected in most samples of crude cereal grain and cereal food commodities such as flour, bran, and oat flakes. the norwegian scientific committee for food safety assessed the risk for adverse effects of deoxynivalenol (don) in different age groups of the domestic population. this review presents the main results from the risk assessment, supplemented with some recently published data. impairment of the immune sys ...	2017	28165414
genome-wide exonic small interference rna-mediated gene silencing regulates sexual reproduction in the homothallic fungus fusarium graminearum.	various ascomycete fungi possess sex-specific molecular mechanisms, such as repeat-induced point mutations, meiotic silencing by unpaired dna, and unusual adenosine-to-inosine rna editing, for genome defense or gene regulation. using a combined analysis of functional genetics and deep sequencing of small noncoding rna (srna), mrna, and the degradome, we found that the sex-specifically induced exonic small interference rna (ex-sirna)-mediated rna interference (rnai) mechanism has an important rol ...	2017	28146558
the cyclase-associated protein fgcap1 has both protein kinase a-dependent and -independent functions during deoxynivalenol production and plant infection in fusarium graminearum.	fusarium graminearum is a causal agent of wheat scab and a producer of the trichothecene mycotoxin deoxynivalenol (don). the expression of trichothecene biosynthesis (tri) genes and don production are mainly regulated by the cyclic adenosine monophosphate-protein kinase a (camp-pka) pathway and two pathway-specific transcription factors (tri6 and tri10). interestingly, deletion mutants of tri6 show reduced expression of several components of camp signalling, including the fgcap1 adenylate-bindin ...	2017	28142217
transcription factor xpp1 is a switch between primary and secondary fungal metabolism.	fungi can produce a wide range of chemical compounds via secondary metabolism. these compounds are of major interest because of their (potential) application in medicine and biotechnology and as a potential source for new therapeutic agents and drug leads. however, under laboratory conditions, most secondary metabolism genes remain silent. this circumstance is an obstacle for the production of known metabolites and the discovery of new secondary metabolites. in this study, we describe the dual r ...	2017	28074041
evidence of an unidentified extracellular heat-stable factor produced by lysobacter enzymogenes (oh11) that degrade fusarium graminearum ph1 hyphae.	lysobacter enzymogenes oh11 produces heat-stable antifungal factor (hsaf) and lytic enzymes possessing antifungal activity. this study bio-prospected for other potential antifungal factors besides those above. the cells and extracellular metabolites of l. enzymogenes oh11 and the mutants δchia, δchib, δchic, δclp, δpks, and δpila were examined for antifungal activity against fusarium graminearum ph1, the causal agent of fusarium head blight (fhb). results evidenced that oh11 produces an unidenti ...	2017	28213660
ptr2 peptide transporters in fusarium graminearum influence secondary metabolite production and sexual development.	putative proton coupled di-peptide transporters, ptr2s, are found in filamentous fungi in different numbers and their function during fungal development and plant infection is unresolved. in fusarium graminearum, the cause of head blight in cereals, we identified four putative ptr2 transporters (fgptr2a-d). the genes did not cluster together in phylogenetic analyses and only fgptr2a and fgptr2c were able to complement a ptr2 deficient yeast mutant in uptake of di-peptides. all fgptr2s are contin ...	2017	28390508
the insect-derived antimicrobial peptide metchnikowin targets fusarium graminearum β(1,3)glucanosyltransferase gel1, which is required for the maintenance of cell wall integrity.	antimicrobial peptides (amps) are essential components of the insect innate immune system. their diversity provides protection against a broad spectrum of microbes and they have several distinct modes of action. insect-derived amps are currently being developed for both medical and agricultural applications, and their expression in transgenic crops confers resistance against numerous plant pathogens. the antifungal peptide metchnikowin (mtk), which was originally discovered in the fruit fly dros ...	2017	27811341
synthesis, characterization and antifungal efficacy of c-coordinated o-carboxymethyl chitosan cu(ii) complexes.	a novel type of o-carboxymethyl chitosan schiff bases (o-cspx) was synthesized via a condensation reaction. after the coordination reaction of cupric ions, cu(ii) complexes (o-cspx-cu) were achieved. the theoretical structure of o-cspx-cu calculated by gaussian 09 reveals that the copper ions underwent dsp(2) hybridization, coordinated by the carbon atom in the p-π conjugate group and the oxygen atoms in the acetate ion. then, the structures were confirmed by ft-ir, (1)h nmr, cp-mas (13)c nmr, e ...	2017	28115106
production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome.	through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase-named chi18h8 and belonging to family 18 glycosyl hydrolases-was previously discovered. the initial extremely low yield of chi18h8 recombinant production and purification from escherichia coli cells (21 μg/g cell) limited its characterization, thus preventing further investigation on its biotechnological potential.	2017	28137256
fcrav2, a gene with rogdi domain involved in fusarium head blight and crown rot on durum wheat caused by fusarium culmorum.	fusarium culmorum is a soil-borne fungal pathogen able to cause foot and root rot and fusarium head blight on small grain cereals, particularly on wheat and barley. it causes significant yield and quality loss and results in the contamination of kernels with type b trichothecene mycotoxins. knowledge on pathogenicity factors of this fungus is still limited. a transposon tagging approach based on the mimp1/impala double component system has allowed us to select a mutant altered in multiple metabo ...	2017	28322011
synthesis and antifungal activity of novel myrtenal-based 4-methyl-1,2,4-triazole-thioethers.	a series of novel myrtenal derivatives bearing 1,2,4-triazole moiety were designed and synthesized by multi-step reactions in an attempt to develop potent antifungal agents. their structures were confirmed by using uv-vis, ftir, nmr, and esi-ms analysis. antifungal activity of the target compounds was preliminarily evaluated by the in vitro method against fusarium oxysporum f. sp. cucumerinum, physalospora piricola, alternaria solani, cercospora arachidicola, and gibberella zeae at 50 µg/ml. com ...	2017	28125042
a tomatinase-like enzyme acts as a virulence factor in the wheat pathogen fusarium graminearum.	during their interactions with plants, fungal pathogens employ large numbers of pathogenesis-associated molecules including secreted effectors and enzymes that can degrade various defence compounds. however, in many cases, in planta targets of pathogen-produced enzymes remain unknown. we identified a gene in the wheat pathogen fusarium graminearum, encoding a putative enzyme that shows 84% sequence identity to fotom1, a tomatinase produced by the tomato pathogen fusarium oxysporum f. sp. lycoper ...	2017	28109774