Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ultrastructural, metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress. | present study explores physiological, biochemical and proteomic changes in leaves of upland cotton (zms-49) using 500 μm cadmium (cd) along with control. leaves' biomass and chlorophyll pigments decreased at 500 μm cd. cd contents in roots were higher than leaves. levels of ros ( [formula: see text] and h2o2) both in vivo and in vitro and mda contents were significantly increased. chlorophyll parameters (f0, fm, fm(') and fv/fm), total soluble protein contents and apx showed a decline at 500 μm ... | 2015 | 25169734 |
| genomic organization, differential expression, and functional analysis of the spl gene family in gossypium hirsutum. | squamosa promoter binding protein-like (spl) genes encode plant-specific transcription factors that are involved in many fundamental developmental processes. certain spl genes contain sequences complementary to mir156, a microrna (mirna) that plays a role in modulating plant gene expression. in this study, 30 spl genes were identified in the reference genome of gossypium raimondii and 24 ghspls were cloned from gossypium hirsutum. g. raimondii is regarded as the putative contributor of the d-sub ... | 2015 | 25159110 |
| cotton lim domain-containing protein ghplim1 is specifically expressed in anthers and participates in modulating f-actin. | as one form of actin binding protein (abp), lim domain protein can trigger the formation of actin bundles during plant growth and development. in this study, a cdna (designated ghplim1) encoding a lim domain protein with 216 amino acid residues was identified from a cotton flower cdna library. quantitative rt-pcr indicated that ghplim1 is specifically expressed in cotton anthers, and its expression levels are regulated during anther development of cotton. ghplim1:egfp transformed cotton cells di ... | 2015 | 25294521 |
| overexpression of a cotton annexin gene, ghann1, enhances drought and salt stress tolerance in transgenic cotton. | plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. ghann1, which encodes a putative annexin protein, was isolated from a cotton (gossypium hirsutum l. acc 7235) cdna library. tissue-specific expression showed that ghann1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. in vivo and in vitro subcellular localiza ... | 2015 | 25330941 |
| cotton (gossypium hirsutum) 14-3-3 proteins participate in regulation of fibre initiation and elongation by modulating brassinosteroid signalling. | cotton (gossypium hirsutum) fibre is an important natural raw material for textile industry in the world. understanding the molecular mechanism of fibre development is important for the development of future cotton varieties with superior fibre quality. in this study, overexpression of gh14-3-3l in cotton promoted fibre elongation, leading to an increase in mature fibre length. in contrast, suppression of expression of gh14-3-3l, gh14-3-3e and gh14-3-3h in cotton slowed down fibre initiation and ... | 2015 | 25370928 |
| itraq protein profile differential analysis between somatic globular and cotyledonary embryos reveals stress, hormone, and respiration involved in increasing plantlet regeneration of gossypium hirsutum l. | somatic embryo development (sed) in upland cotton shows low frequencies of embryo maturation and plantlet regeneration. progress in increasing the regeneration rate has been limited. here a global analysis of proteome dynamics between globular and cotyledonary embryos was performed using isobaric tags for relative and absolute quantitation to explore mechanisms underlying sed. of 6318 proteins identified by a mass spectrometric analysis, 102 proteins were significantly up-regulated and 107 were ... | 2015 | 25367710 |
| lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton. | the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations. | 2015 | 24777962 |
| dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (gossypium hirsutum). | the soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (rflp), terminal restriction length polymorphism (t-rflp), and cloning-sequencing. the cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. phylogenetic and diversity analysis of sequences obtained from the f ... | 2015 | 24677076 |
| non-destructive high-throughput dna extraction and genotyping methods for cotton seeds and seedlings. | extensive use of targeted pcr-based genotyping is precluded for many plant research laboratories by the cost and time required for dna extraction. using cotton (gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-throughput extraction of dna suitable for pcr-based genotyping of large numbers of individual seeds and seedlings. by sampling only small amounts of cotyledon tissue of ungerminated seed or young seedli ... | 2015 | 25967902 |
| effects of climate change on phenological trends and seed cotton yields in oasis of arid regions. | understanding the effects of climatic change on phenological phases of cotton (gossypium hirsutum l.) in oasis of arid regions may help optimize management schemes to increase productivity. this study assessed the impacts of climatic changes on the phenological phases and productivity of spring cotton. the results showed that climatic warming led the dates of sowing seed, seeding emergence, three-leaf, five-leaf, budding, anthesis, full bloom, cleft boll, boll-opening, boll-opening filling, and ... | 2015 | 25240389 |
| tillage, cropping systems, and nitrogen fertilizer source effects on soil carbon sequestration and fractions. | quantification of soil carbon (c) cycling as influenced by management practices is needed for c sequestration and soil quality improvement. we evaluated the 10-yr effects of tillage, cropping system, and n source on crop residue and soil c fractions at 0- to 20-cm depth in decatur silt loam (clayey, kaolinitic, thermic, typic paleudults) in northern alabama, usa. treatments were incomplete factorial combinations of three tillage practices (no-till [nt], mulch till [mt], and conventional till [ct ... | 2015 | 18453410 |
| effect of high-temperature stress on the growth and seed characteristics of barley and cotton. | the existence of a sensitivity gradient to a uniform high-temperature stress applied at stages during seed maturation can be demonstrated in barley. the germination of freshly harvested seed is depressed following heat stress at 7--10 days after awn emergence, but is enhanced by the same stress applied 3 weeks after awn emergence. the depression is attributed to reduced viability associated with thermal injury. the stimulation following stress at more mature stages of seed development is related ... | 2015 | 1032107 |
| dose and time dependent efficacy alteration of different defoliants on seed cotton yield. | field experiments were conducted during 2012 and 2013 to determine the effect of select defoliants i.e dropp ultra and ethrel, their optimal dose and suitable time of application on earliness and seed cotton yield in 3 american cotton cultivars. mrc7361bgii (3055.2 kg ha(-1)) and mrc7017bgii (2913.0 kg ha(-1)) resulted in significantly improved seed cotton yield as compared to f1 861 (2346.0 kg ha(-1)). dropp ultra? @ 200 ml ha(-1) revealed highest yield (3018.7 kg ha(-1)) followed by ethrel @ 2 ... | 2015 | 26364466 |
| evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (gossypium hirsutum l.) cv. svpr-2. | an efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. as cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. among them, 3% maltose was ... | 2015 | 28626717 |
| fluometuron and pendimethalin runoff from strip and conventionally tilled cotton in the southern atlantic coastal plain. | in the atlantic coastal plain region of southern georgia (usa), cotton (gossypium hirsutum l.) acreage increased threefold in the past decade. to more effectively protect water quality in the region, best management practices are needed that reduce pesticide runoff from fields in cotton production. this study compared runoff of two herbicides, fluometuron [n,n-dimethyl-n'-[3-(trifluoromethyl)-phenyl]-urea] and pendimethalin [n-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitro-benzenamine], from plots in ... | 2015 | 15537934 |
| [disturbed structure and function of chloroplasts during blocked biosynthesis of 5'-aminolevulinic acid in the light]. | xantha-702 mutant of cotton (gossypium hirsutum l.) proved to have blocked synthesis of 5'-aminolevulinic acid in the light. accordingly, mutant leaves accumulated 2-5% chlorophyll of baseline. mutant plants demonstrated disturbed production of pigment-protein complexes of photosystems i (psi) and ii (psii) and generation of the chloroplast membrane system blocked at the early stages, largely, at the stages of vesicles and single short thylakoid. the functional activity of the psi and psii react ... | 2016 | 17853692 |
| gbexpatr, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. | cotton provides us the most important natural fibre. high fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from gossypium barbadense to gossypium hirsutum is an important breeding strategy. we previously analysed the g. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from g. barbadense, gbexpa2 and gbexpatr. gbexpa2 (from the dt genome) is a classical α-e ... | 2016 | 26269378 |
| physiological performance and differential expression profiling of genes associated with drought tolerance in root tissue of four contrasting varieties of two gossypium species. | root growth in drying soil is generally limited by a combination of mechanical impedance and water stress. as the major function of root tissue is water and nutrient uptake, so it imparts an important role in plant growth and stress management. previously, we have studied physiological performance and expression profiling of gene associated with drought tolerance in leaf tissue of four cotton varieties. here, we have further continued our studies with the root tissue of these varieties. the goss ... | 2016 | 25802007 |
| identification of micro-rnas in cotton. | the plant genome has conserved small non-coding micrornas (mirnas) genes about 20-24 nucleotides long. they play a vital role in the gene regulation at various stages of plant life. their conserved nature among the various organisms not only suggests their early evolution in eukaryotes but also makes them a good source of new mirna discovery by homology search using bioinformatics tools. a systematic search approach was used for interspecies orthologues of mirna precursors, from known sequences ... | 2016 | 18603441 |
| ascorbate alleviates fe deficiency-induced stress in cotton (gossypium hirsutum) by modulating aba levels. | fe deficiency causes significant losses to crop productivity and quality. to understand better the mechanisms of plant responses to fe deficiency, we used an in vitro cotton ovule culture system. we found that fe deficiency suppressed the development of ovules and fibers, and led to tissue browning. rna-seq analysis showed that the myo-inositol and galacturonic acid pathways were activated and cytosolic apx (ascorbate peroxidase) was suppressed in fe-deficient treated fibers, which increased asc ... | 2016 | 28101095 |
| cotip: cotton tilling platform, a resource for plant improvement and reverse genetic studies. | cotton is cultivated worldwide for its white fiber, of which around 90% is tetraploid upland cotton (gossypium hirsutum l.) carrying both a and d genome. since centuries, yield increasing efforts for the cotton crop by conventional breeding approaches have caused an extensive erosion of natural genetic variability. mutation based improvement strategies provide an effective way of creating new allelic variations. targeting induced local lesions in genomes (tilling) provides a mutation based rever ... | 2016 | 28082993 |
| frankliniella fusca resistance to neonicotinoid insecticides: an emerging challenge for cotton pest management in the eastern united states. | over the past two decades, neonicotinoid seed treatments have become the primary method to manage tobacco thrips, frankliniella fusca hinds, on seedling cotton. because this insect is highly polyphagous and the window of insecticide exposure is short, neonicotinoid resistance was expected to pose a minimal risk. however, reports of higher than expected f. fusca seedling damage in seed-treated cotton fields throughout the mid-south and southeast us production regions suggested neonicotinoid resis ... | 2016 | 26775623 |
| lightweight males of podisus nigrispinus (heteroptera: pentatomidae) neglect lightweight females due low reproductive fitness. | sexual choice by male stink bugs is important because females that experience food shortages lay fewer eggs with lower viability compared with well-fed females. in this study, we investigated whether podisus nigrispinus (dallas) (heteroptera: pentatomidae) males fed with a low-quality diet during its nymphal stage show selectivity for sexual partners resulting in high-quality progeny. lightweight males and females were obtained from nymphs fed weekly with tenebrio molitor l. (coleoptera: tenebri ... | 2016 | 27599098 |
| monopodial and sympodial branching architecture in cotton is differentially regulated by the gossypium hirsutum single flower truss and self-pruning orthologs. | domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a day-neutral annual row-crop. residual perennial traits, however, complicate irrigation and crop management, and more determinate architectures are desired. cotton simultaneously maintains robust monopodial indeterminate shoots and sympodial determinate shoots. we questioned if and how the flowering locus t/single flower truss (sft)-like and terminal flower1/self-pruning (sp)-like genes cont ... | 2016 | 27292411 |
| resistance to glufosinate is proportional to phosphinothricin acetyltransferase expression and activity in libertylink(®) and widestrike(®) cotton. | insertion of the gene encoding phosphinothricin acetyltransferase (pat) has resulted in cotton plants resistant to the herbicide glufosinate. however, the lower expression and commensurate reduction in pat activity is a key factor in the low level of injury observed in the widestrike(®) cotton and relatively high level of resistance observed in libertylink(®) cotton. libertylink(®) cotton cultivars are engineered for glufosinate resistance by overexpressing the bar gene that encodes phosphinothr ... | 2016 | 26733464 |
| partial life history of chrysodeixis includens (lepidoptera: noctuidae) on summer hosts. | the soybean looper, chrysodeixis includens (walker) (lepidoptera: noctuidae), is a major defoliating pest of soybeans, glycine max (l.) merrill, in louisiana. however, other alternate host crops in the agroecosystem have the potential to impact c. includens populations. life table statistics of c. includens on four host plants were evaluated. c. includens larvae were fed leaves of three cotton gossypium hirsutum l. cultivars 'dp 143 b2rf,' 'dp 174 rf,' and 'phy 485 wrf'; cowpea vigna unguiculata ... | 2016 | 27375294 |
| evolution of gene duplication in plants. | ancient duplication events and a high rate of retention of extant pairs of duplicate genes have contributed to an abundance of duplicate genes in plant genomes. these duplicates have contributed to the evolution of novel functions, such as the production of floral structures, induction of disease resistance, and adaptation to stress. additionally, recent whole-genome duplications that have occurred in the lineages of several domesticated crop species, including wheat (triticum aestivum), cotton ... | 2016 | 27288366 |
| quantitative trait loci analysis of verticillium wilt resistance in interspecific backcross populations of gossypium hirsutum × gossypium barbadense. | verticillium wilt (vw) caused by verticillium dahliae (kleb) is one of the most destructive diseases of cotton. the identification of highly resistant qtls or genes in the whole cotton genome is quite important for developing a vw-resistant variety and for further molecular design breeding. | 2016 | 27814678 |
| spatio-temporal, genotypic, and environmental effects on plant soluble protein and digestible carbohydrate content: implications for insect herbivores with cotton as an exemplar. | plant soluble protein and digestible carbohydrate content significantly affect insect herbivore fitness, but studies reporting plant protein and carbohydrate content are rare. instead, the elements nitrogen and carbon often are used as surrogates for plant protein and digestible carbohydrate content, respectively. however, this is problematic for two reasons. first, carbon is found in all organic molecules, which precludes strong correlations with ecologically important dietary macronutrients (e ... | 2016 | 27738861 |
| identification of chromosome segment substitution lines of gossypium barbadense introgressed in g. hirsutum and quantitative trait locus mapping for fiber quality and yield traits. | chromosome segment substitution lines mbi9804, mbi9855, mbi9752, and mbi9134, which were obtained by advanced backcrossing and continuously inbreeding from an interspecific cross between ccri36, a cultivar of upland cotton (gossypium hirsutum) as the recurrent parent, and hai1, a cultivar of sea island cotton (g. barbadense) as the donor parent, were used to construct a multiple parent population of (mbi9804×mbi9855)×(mbi9752×mbi9134). the segregating generations of double-crossed f1 and f2 and ... | 2016 | 27603312 |
| genetic effects and heterosis of yield and yield component traits based on gossypium barbadense chromosome segment substitution lines in two gossypium hirsutum backgrounds. | we hybridized 10 chromosome segment substitution lines (cssls) each from two cssl populations and produced 50 f1 hybrids according to north carolina design ii. we analyzed the genetic effects and heterosis of yield and yield components in the f1 hybrids and parents in four environments via the additive-dominance genetic model. yield and yield components of the cssls were controlled by combined additive and dominance effects, and lint percentage was mainly controlled by additive effects, but boll ... | 2016 | 27348815 |
| independent domestication of two old world cotton species. | domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ... | 2016 | 27289095 |
| a comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a gossypium hirsutum × gossypium barbadense population. | upland cotton (gossypium hirsutum l.) is the most important fiber crop, and its lint-yield improvement is impeded due to its narrow genetic base and the lack of understanding of the genetic basis of yield. backcross inbred lines (bils) or near-isogenic lines (nils) in the same genetic background differing in lint yield, developed through advanced backcrossing, provide an important genomic resource to study the molecular genetic basis of lint yield. in the present study, a high-yield (hy) group a ... | 2016 | 27256327 |
| to be a flower or fruiting branch: insights revealed by mrna and small rna transcriptomes from different cotton developmental stages. | the architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. nulliplex branch is a useful phenotype to study cotton architecture. we used rna sequencing to obtain mrna and mirna profiles from nulliplex- and normal-branch cotton at three developmental stages. the differentially expressed genes (degs) and mirnas were identified that preferentially/specifica ... | 2016 | 26983497 |
| genetic diversity of the two commercial tetraploid cotton species in the gossypium diversity reference set. | a diversity reference set has been constructed for the gossypium accessions in the us national cotton germplasm collection to facilitate more extensive evaluation and utilization of accessions held in the collection. a set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid gossypium accessions representative of the range of diversity of the improved and wild accessions of g. hirsutum and g. barbadense. the reference set contained 410 g. barbad ... | 2016 | 26774060 |
| breeding potential of introgression lines developed from interspecific crossing between upland cotton (gossypium hirsutum) and gossypium barbadense: heterosis, combining ability and genetic effects. | upland cotton (gossypium hirstum l.), which produces more than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. introducing germplasm from exotic sources especially from another cultivated tetraploid g. barbadense l. can broaden the genetic base of upland cotton. however, the breeding potential of introgression lines (ils) in upland cotton with g. barbadense germplasm integration has not been well addressed. this study involved six ils ... | 2016 | 26730964 |
| relative fitness of helicoverpa armigera (lepidoptera: noctuidae) on seven host plants: a perspective for ipm in brazil. | the cotton bollworm helicoverpa armigera (hübner) (lepidoptera: noctuidae) is a widespread pest of many cultivated and wild plants in europe, africa, asia, and australia. in 2013, this species was reported in brazil, attacking various host crops in the midwestern and northeastern regions of the country and is now found countrywide. aiming to understand the effects of different host plants on the life cycle of h. armigera, we selected seven species of host plants that mature in different seasons ... | 2016 | 26798139 |
| identification of stable quantitative trait loci (qtls) for fiber quality traits across multiple environments in gossypium hirsutum recombinant inbred line population. | the identification of quantitative trait loci (qtls) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (mas). in the present study, we used 28,861 simple sequence repeat (ssr) markers, which included 12,560 gossypium raimondii (d genome) sequence-based ssr markers to identify polymorphism between two upland cotton strains 0-153 and sgk9708. a total of 851 polymorphic primers were finally selected and used to genotype ... | 2016 | 26951621 |
| genetic gains from selection for fiber traits in gossypium hirsutum l. | brazil is among the five largest producers of cotton in the world, cultivating the species gossypium hirsutum l. r. latifolium hutch. the cultivars should have good fiber quality as well as yield. genetic improvement of fiber traits requires the study of the genetic structure of the populations under improvement, leading to the identification of promising parent plants. to this end, it is important to acquire some information, such as estimates of genetic variance components and heritability coe ... | 2016 | 27886330 |
| polymorphism analysis of multi-parent advanced generation inter-cross (magic) populations of upland cotton developed in china. | upland cotton (gossypium hirsutum l.) is an important cash crop that provides renewable natural fiber worldwide. currently limited genetic base leads to a decrease in upland cotton genetic diversity. multi-parent advance generation inter-cross (magic) populations can be used to evaluate complex agronomic traits in crops. in this study, we developed an upland cotton magic population. a total of 258 magic population lines and their twelve founder lines were analyzed, using 432 pairs of simple sequ ... | 2016 | 28002582 |
| comparison of ionomic and metabolites response under alkali stress in old and young leaves of cotton (gossypium hirsutum l.) seedlings. | soil salinization is an important agriculture-related environmental problem. alkali stress and salt stress strongly influence the metabolic balance in plants. salt and alkali stresses exert varied effects on old and young tissues, which display different adaptive strategies. in this study, we used cotton (gossypium hirsutum l.) plants as experimental material to investigate whether alkali stress induces ionic and metabolism changes in old and young leaves of cotton plants exposed to alkali stres ... | 2016 | 27933088 |
| high-density linkage map construction and qtl analysis for earliness-related traits in gossypium hirsutum l. | gossypium hirsutum l., or upland cotton, is an important renewable resource for textile fiber. to enhance understanding of the genetic basis of cotton earliness, we constructed an intra-specific recombinant inbred line population (ril) containing 137 lines, and performed linkage map construction and quantitative trait locus (qtl) mapping. | 2016 | 27835938 |
| comparative genome-wide analysis of the malate dehydrogenase gene families in cotton. | malate dehydrogenases (mdhs) play crucial roles in the physiological processes of plant growth and development. in this study, 13 and 25 mdh genes were identified from gossypium raimondii and gossypium hirsutum, respectively. using these and 13 previously reported gossypium arboretum mdh genes, a comparative molecular analysis between identified mdh genes from g. raimondii, g. hirsutum, and g. arboretum was performed. based on multiple sequence alignments, cotton mdhs were divided into five subg ... | 2016 | 27829020 |
| genome-wide identification and expression analysis of stress-associated proteins (saps) containing a20/an1 zinc finger in cotton. | stress-associated proteins (saps) containing the a20/an1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. nevertheless, few studies have focused on the sap gene family in cotton. to explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of saps in tetraploid gossypium hirsutum l. tm-1 (ad1). a total of 37 genes encoding saps were identified, 36 of which were duplicated in the ... | 2016 | 27681253 |
| development, genetic mapping and qtl association of cotton phya, phyb, and hy5-specific caps and dcaps markers. | among snp markers that become increasingly valuable in molecular breeding of crop plants are the caps and dcaps markers derived from the genes of interest. to date, the number of such gene-based markers is small in polyploid crop plants such as allotetraploid cotton that has a- and d-sub-genomes. the objective of this study was to develop and map new caps and dcaps markers for cotton developmental-regulatory genes that are important in plant breeding programs. | 2016 | 27776497 |
| small rna-mediated responses to low- and high-temperature stresses in cotton. | micrornas (mirnas) are one class of endogenous non-coding rnas modulating the expression of target genes involved in plant development and stress tolerance, by degrading mrna or repressing translation. in this study, small rna and mrna degradome sequencing were used to identify low- and high-temperature stress-responsive mirnas and their targets in cotton (gossypium hirsutum). cotton seedlings were treated under different temperature conditions (4, 12, 25, 35, and 42 °c) and then the effects wer ... | 2016 | 27752116 |
| identification and functional analysis of micrornas involved in the anther development in cotton genic male sterile line yu98-8a. | hybrid vigor contributes in a large way to the yield and quality of cotton (gossypium hirsutum) fiber. although micrornas play essential regulatory roles in flower induction and development, it is still unclear if micrornas are involved in male sterility, as the regulatory molecular mechanisms of male sterility in cotton need to be better defined. in this study, two independent small rna libraries were constructed and sequenced from the young buds collected from the sporogenous cell formation to ... | 2016 | 27739413 |
| a raf-like mapkkk gene, ghraf19, negatively regulates tolerance to drought and salt and positively regulates resistance to cold stress by modulating reactive oxygen species in cotton. | mitogen-activated protein kinase kinase kinases (mapkkks) function at the top level of mapk cascades and play important roles in plant development and stress responses. although mapkkks comprise the largest family in the mapk cascades, very few raf-like mapkkks have been functionally identified, especially in the economically important crop cotton. in this study, a raf-like mapkkk gene, ghraf19, was characterized for the first time in cotton. our data show that the expression of ghraf19 was inhi ... | 2016 | 27717463 |
| characterization of eleven monosomic alien addition lines added from gossypium anomalum to gossypium hirsutum using improved gish and ssr markers. | gossypium anomalum (bb genome) possesses the desirable characteristics of drought tolerance, resistance to diseases and insect pests, and the potential for high quality fibers. however, it is difficult to transfer the genes associated with these desirable traits into cultivated cotton (g. hirsutum, aadd genome). monosomic alien addition lines (maals) can be used as a bridge to transfer desired genes from wild species into g. hirsutum. in cotton, however, the high number and smaller size of the c ... | 2016 | 27717331 |
| ghabf2, a bzip transcription factor, confers drought and salinity tolerance in cotton (gossypium hirsutum l.). | the bzip transcription factor (tf) act as an important regulator for the abscisic acid (aba) mediated abiotic stresses signaling pathways in plants. here, we reported the cloning and characterization of ghabf2, encoding for typical cotton bzip tf. overexpression of ghabf2 significantly improved drought and salt stress tolerance both in arabidopsis and cotton. however, silencing of ghabf2 made transgenic cotton sensitive to peg osmotic and salt stress. expression of ghabf2 was induced by drought ... | 2016 | 27713524 |
| na(+) compartmentalization related to salinity stress tolerance in upland cotton (gossypium hirsutum) seedlings. | the capacity for ion compartmentalization among different tissues and cells is the key mechanism regulating salt tolerance in plants. in this study, we investigated the ion compartmentalization capacity of two upland cotton genotypes with different salt tolerances under salt shock at the tissue, cell and molecular levels. we found that the leaf glandular trichome could secrete more salt ions in the salt-tolerant genotype than in the sensitive genotype, demonstrating the excretion of ions from ti ... | 2016 | 27698468 |
| ghcam7-like, a calcium sensor gene, influences cotton fiber elongation and biomass production. | calcium signaling regulates many developmental processes in plants. calmodulin (cam) is one of the most conserved calcium sensors and has a flexible conformation in eukaryotes. the molecular functions of cam are unknown in cotton, which is a major source of natural fiber. in this study, a gossypium hirsutum l.cam7-like gene was isolated from upland cotton. bioinformatics analysis indicated that the ghcam7-like gene was highly conserved as compared with arabidopsis atcam7. the ghcam7-like gene sh ... | 2016 | 27669397 |
| impact of insect management on population dynamics and insecticide resistance of tarnished plant bug (hemiptera: miridae). | while transgenic plants targeting lepidopteran and coleopteran insects have been available for almost 20 yr, there are no transgenic crops that target hemipteran insects such as tarnished plant bug, lygus lineolaris (palisot de beauvois), though at least one company lists potential products in advanced stages of development. a resistance management model for the u.s. mid-south was developed to aid in resistance risk assessments for transgenic crops targeting l. lineolaris, and validated against ... | 2016 | 27651293 |
| histone modifications define expression bias of homoeologous genomes in allotetraploid cotton. | histone modifications regulate gene expression in eukaryotes, but their roles in gene expression changes in interspecific hybrids or allotetraploids are poorly understood. histone modifications can be mapped by immunostaining of metaphase chromosomes at the single cell level and/or by chromatin immunoprecipitation-sequencing (chip-seq) for analyzing individual genes. here, we comparatively analyzed immunostained metaphase chromosomes and chip-seq of individual genes, which revealed a chromatin b ... | 2016 | 27637746 |
| proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress. | drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. to determine the effects of drought stress on cotton seedlings, we used tw ... | 2016 | 26941218 |
| transgenic cotton plants expressing cry1ia12 toxin confer resistance to fall armyworm (spodoptera frugiperda) and cotton boll weevil (anthonomus grandis). | gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including cry toxins, proteinase inhibitors, and toxic peptides, among others. in the present study, we developed transgenic cotton plants by fertilized floral buds injection (through ... | 2016 | 26925081 |
| global analysis of the developmental dynamics of gossypium hirsutum based on strand-specific transcriptome. | cotton is an economically important crop that provides both natural fiber and by-products such as oil and protein. its global gene expression could provide insight into the biological processes underlying growth and development, which involve suites of genes expressed with temporal and spatial control by regulatory networks. generally, the goal for cotton breeding is improvement of the fiber; thus, most previous research has focused on identifying genes specific to the fiber. however, seeds may ... | 2016 | 26892265 |
| diversity in betasatellites associated with cotton leaf curl disease during source-to-sink movement through a resistant host. | cotton leaf curl is devastating disease of cotton characterized by leaf curling, vein darkening and enations. the disease symptoms are induced by dna satellite known as cotton leaf curl multan betasatellite (clcumub), dominant betasatellite in cotton but another betasatellite known as chili leaf curl betasatellite (chlcb) is also found associated with the disease. grafting experiment was performed to determine if host plant resistance is determinant of dominant population of betasatellite in cot ... | 2016 | 26889114 |
| mapping by sequencing in cotton (gossypium hirsutum) line md52ne identified candidate genes for fiber strength and its related quality attributes. | three qtl regions controlling three fiber quality traits were validated and further fine-mapped with 27 new single nucleotide polymorphism (snp) markers. transcriptome analysis suggests that receptor-like kinases found within the validated qtls are potential candidate genes responsible for superior fiber strength in cotton line md52ne. fiber strength, length, maturity and fineness determine the market value of cotton fibers and the quality of spun yarn. cotton fiber strength has been recognized ... | 2016 | 26883043 |
| relationship between potassium fertilization and nitrogen metabolism in the leaf subtending the cotton (gossypium hirsutum l.) boll during the boll development stage. | the nitrogen (n) metabolism of the leaf subtending the cotton boll (lscb) was studied with two cotton (gossypium hirsutum l.) cultivars (simian 3, low-k tolerant; siza 3, low-k sensitive) under three levels of potassium (k) fertilization (k0: 0 g k2o plant(-1), k1: 4.5 k2o plant(-1) and k2: 9.0 g k2o plant(-1)). the results showed that total dry matter increased by 13.1-27.4% and 11.2-18.5% under k supply for simian 3 and siza 3. boll biomass and boll weight also increased significantly in k1 an ... | 2016 | 26874296 |
| h2o2 and aba signaling are responsible for the increased na+ efflux and water uptake in gossypium hirsutum l. roots in the non-saline side under non-uniform root zone salinity. | non-uniform root salinity increases the na(+)efflux, water use, and growth of the root in non-saline side, which may be regulated by some form of signaling induced by the high-salinity side. however, the signaling and its specific function have remained unknown. using a split-root system to simulate a non-uniform root zone salinity in gossypium hirsutum l., we showed that the up-regulated expression of sodium efflux-related genes (sos1, sos2, pma1, and pma2) and water uptake-related genes (pip1 ... | 2016 | 26862153 |
| fruiting branch k(+) level affects cotton fiber elongation through osmoregulation. | potassium (k) deficiency in cotton plants results in reduced fiber length. as one of the primary osmotica, k(+) contributes to an increase in cell turgor pressure during fiber elongation. therefore, it is hypothesized that fiber length is affected by k deficiency through an osmotic pathway, so in 2012 and 2013, an experiment was conducted to test this hypothesis by imposing three potassium supply regimes (0, 125, 250 kg k ha(-1)) on a low-k-sensitive cultivar, siza 3, and a low-k-tolerant cultiv ... | 2016 | 26834777 |
| comparative fiber property and transcriptome analyses reveal key genes potentially related to high fiber strength in cotton (gossypium hirsutum l.) line md52ne. | individual fiber strength is an important quality attribute that greatly influences the strength of the yarn spun from cotton fibers. fiber strength is usually measured from bundles of fibers due to the difficulty of reliably measuring strength from individual cotton fibers. however, bundle fiber strength (bfs) is not always correlated with yarn strength since it is affected by multiple fiber properties involved in fiber-to-fiber interactions within a bundle in addition to the individual fiber s ... | 2016 | 26833213 |
| small interfering rnas from bidirectional transcripts of ghmml3_a12 regulate cotton fiber development. | natural antisense transcripts (nats) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. in this research, we investigated the function of small rna derived from a nat in fiber cell development. using a map-based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene (n1 ) encoding a mybmixta-like transcription factor 3 (mml3)/ghmyb25-like in chromosome a12, ... | 2016 | 26832840 |
| functional characterization of a dihydroflavanol 4-reductase from the fiber of upland cotton (gossypium hirsutum). | dihydroflavanol 4-reductase (dfr) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (pas)) biosynthesis, however it is not characterized in cotton yet. in present reports, a dfr cdna homolog (designated as ghdfr1) was cloned from developing fibers of upland cotton. silencing ghdfr1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and pas. more interestingly, based on lc-ms analysis, two pa monomers, (-)-epi ... | 2016 | 26821011 |
| field-based high-throughput plant phenotyping reveals the temporal patterns of quantitative trait loci associated with stress-responsive traits in cotton. | the application of high-throughput plant phenotyping (htpp) to continuously study plant populations under relevant growing conditions creates the possibility to more efficiently dissect the genetic basis of dynamic adaptive traits. toward this end, we employed a field-based htpp system that deployed sets of sensors to simultaneously measure canopy temperature, reflectance, and height on a cotton (gossypium hirsutum l.) recombinant inbred line mapping population. the evaluation trials were conduc ... | 2016 | 26818078 |
| a cotton mirna is involved in regulation of plant response to salt stress. | the present study functionally identified a new microrna (microrna ovual line 5, mirnvl5) with its target gene ghchr from cotton (gossypium hirsutum). the sequence of mirnvl5 precursor is 104 nt long, with a well developed secondary structure. ghchr contains two dc1 and three phd cys/his-rich domains, suggesting that ghchr encodes a zinc-finger domain-containing transcription factor. mirnvl5 and ghchr express at various developmental stages of cotton. under salt stress (50-400 mm nacl), mirnvl5 ... | 2016 | 26813144 |
| influence of dual-bt protein corn on bollworm, helicoverpa zea (boddie), survivorship on bollgard ii cotton. | similar cry proteins are expressed in both bt corn, zea mays l., and cotton, gossypium hirsutum (l.), commercial production systems. at least one generation of corn earworm, helicoverpa zea (boddie), completes development on field corn in the mid-south before dispersing across the landscape into other crop hosts like cotton. a concern is that bt corn hybrids may result in selection for h. zea populations with a higher probability of causing damage to bt cotton. the objective of this study was to ... | 2016 | 26809264 |
| two-dimensional gel electrophoresis-based analysis provides global insights into the cotton ovule and fiber proteomes. | proteomic analysis of upland cotton was performed to profile the global detectable proteomes of ovules and fibers using two-dimensional electrophoresis (2de). a total of 1,203 independent protein spots were collected from representative 2de gels, which were digested with trypsin and identified by matrix-assisted laser desorption and ionization-time-offlight/ time-of-flight (maldi-tof/tof) mass spectrometry. the mass spectrometry or tandem mass spectrometry (ms or ms/ms) data were then searched a ... | 2016 | 26803300 |
| cotton ghmyb7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic arabidopsis. | the secondary cell wall in mature cotton fibers contains over 90% cellulose with low quantities of xylan and lignin. however, little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers. in this study, we characterized an r2r3-myb transcription factor, ghmyb7, in cotton. ghmyb7 is expressed at a high level in developing fibers and encodes a myb protein that is targeted to the cell nucleus and has transcriptional activation activity. ectopic expression of ghmyb7 ... | 2016 | 26803299 |
| genome-wide transcriptomic and proteomic analyses of bollworm-infested developing cotton bolls revealed the genes and pathways involved in the insect pest defence mechanism. | cotton bollworm, helicoverpa armigera, is a major insect pest that feeds on cotton bolls causing extensive damage leading to crop and productivity loss. in spite of such a major impact, cotton plant response to bollworm infection is yet to be witnessed. in this context, we have studied the genome-wide response of cotton bolls infested with bollworm using transcriptomic and proteomic approaches. further, we have validated this data using semi-quantitative real-time pcr. comparative analyses have ... | 2016 | 26799171 |
| online co2 and h2 o oxygen isotope fractionation allows estimation of mesophyll conductance in c4 plants, and reveals that mesophyll conductance decreases as leaves age in both c4 and c3 plants. | mesophyll conductance significantly, and variably, limits photosynthesis but we currently have no reliable method of measurement for c4 plants. an online oxygen isotope technique was developed to allow quantification of mesophyll conductance in c4 plants and to provide an alternative estimate in c3 plants. the technique is compared to an established carbon isotope method in three c3 species. mesophyll conductance of c4 species was similar to that in the c3 species measured, and declined in both ... | 2016 | 26778088 |
| confamiliar transferability of simple sequence repeat (ssr) markers from cotton (gossypium hirsutum l.) and jute (corchorus olitorius l.) to twenty two malvaceous species. | cross-species transferability is a quick and economic method to enrich ssr database, particularly for minor crops where little genomic information is available. however, transferability of ssr markers varies greatly between species, genera and families of plant species. we assessed confamiliar transferability of ssr markers from cotton (gossypium hirsutum) and jute (corchorus olitorius) to 22 species distributed in different taxonomic groups of malvaceae. all the species selected were potential ... | 2016 | 28330135 |
| metabolic engineering of cottonseed oil biosynthesis pathway via rna interference. | cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (vitamine) as well as unsaturated fatty acid. however, the cottonseed oil content of cultivated cotton (gossypium hirsutum) is only around 20%. in this study, we modified the accumulation of oils by the down-regulation of phosphoenolpyruvate carboxylase 1 (ghpepc1) via rna interference in transgenic cotton plants. the qrt-pcr and enzyme activity ass ... | 2016 | 27620452 |
| genome-wide characterization and expression analysis of myb transcription factors in gossypium hirsutum. | myb family proteins are one of the most abundant transcription factors in the cotton plant and play diverse roles in cotton growth and evolution. previously, few studies have been conducted in upland cotton, gossypium hirsutum. the recent release of the g. hirsutum genome sequence provides a great opportunity to identify and characterize the entire upland cotton myb protein family. | 2016 | 27613381 |
| cloning and functional analysis of the promoter of an ascorbate oxidase gene from gossypium hirsutum. | apoplastic ascorbate oxidase (ao) plays significant roles in plant cell growth. however, the mechanism of underlying the transcriptional regulation of ao in gossypium hirsutum remains unclear. here, we obtained a 1,920-bp promoter sequence from the gossypium hirsutum ascorbate oxidase (ghao1) gene, and this ghao1 promoter included a number of known cis-elements. promoter activity analysis in overexpressing pghao1::gfp-gus tobacco (nicotiana benthamiana) showed that the ghao1 promoter exhibited h ... | 2016 | 27597995 |
| identification and characterization of the ghhsp20 gene family in gossypium hirsutum. | in higher plants, heat shock protein 20 (hsp20) plays crucial roles in growth, development and responses to abiotic stresses. in this study, 94 ghhsp20 genes were identified in g. hirsutum, and these genes were phylogenetically clustered into 14 subfamilies. out of these, 73 paralogous gene pairs remained in conserved positions on segmental duplicated blocks and only 14 genes clustered into seven tandem duplication event regions. transcriptome analysis showed that 82 ghhsp20 genes were expressed ... | 2016 | 27580529 |
| identification of favorable snp alleles and candidate genes for traits related to early maturity via gwas in upland cotton. | early maturity is one of the most important and complex agronomic traits in upland cotton (gossypium hirsutum l). to dissect the genetic architecture of this agronomically important trait, a population consisting of 355 upland cotton germplasm accessions was genotyped using the specific-locus amplified fragment sequencing (slaf-seq) approach, of which a subset of 185 lines representative of the diversity among the accessions was phenotypically characterized for six early maturity traits in four ... | 2016 | 27576450 |
| the ghtt2_a07 gene is linked to the brown colour and natural flame retardancy phenotypes of lc1 cotton (gossypium hirsutum l.) fibres. | some naturally coloured brown cotton fibres from accessions of gossypium hirsutum l. can be used to make textiles with enhanced flame retardancy (fr). several independent brown fibre loci have been identified and mapped to chromosomes, but the underlying genes have not yet been identified, and the mechanism of lint fibre fr is not yet fully understood. in this study, we show that both the brown colour and enhanced fr of the lc1 lint colour locus are linked to a 1.4mb inversion on chromosome a07 ... | 2016 | 27567364 |
| characterization and functional analysis of pebp family genes in upland cotton (gossypium hirsutum l.). | upland cotton (gossypium hirsutum l.) is a naturally occurring photoperiod-sensitive perennial plant species. however, sensitivity to the day length was lost during domestication. the phosphatidylethanolamine-binding protein (pebp) gene family, of which three subclades have been identified in angiosperms, functions to promote and suppress flowering in photoperiod pathway. recent evidence indicates that pebp family genes play an important role in generating mobile flowering signals. we isolated h ... | 2016 | 27552108 |
| mapping qtls for drought tolerance in an f2:3 population from an inter-specific cross between gossypium tomentosum and gossypium hirsutum. | cotton is one of the most important natural fiber crops in the world. its growth and yield is greatly limited by drought. a quantitative trait locus (qtl) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (gossypium spp) using 188 f2:3 lines developed from an inter-specific cross between a wild cotton species, g. tomentosum, and an upland cotton, g. hirsutum (cri-12). a genetic map was constructed using 1295 simple sequence repeat markers, which amp ... | 2016 | 27525919 |
| de novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (g. hirsutum l.). | plant regeneration via somatic embryogenesis (se) is the key step for genetic improvement of cotton (gossypium hirsutum l.) through genetic engineering mediated by agrobacteria, but the molecular mechanisms underlying se in cotton is still unclear. here, rna-sequencing was used to analyze the genes expressed during se and their expression dynamics using rnas isolated from non-embryogenic callus (nec), embryogenic callus (ec) and somatic embryos (ses). a total of 101, 670 unigenes were de novo as ... | 2016 | 27511192 |
| response and tolerance mechanism of cotton gossypium hirsutum l. to elevated temperature stress: a review. | cotton is an important multipurpose crop which is highly sensitive to both biotic and abiotic stresses. proper management of this cash crop requires systematic understanding of various environmental conditions that are vital to yield and quality. high temperature stress can severely affect the viability of pollens and anther indehiscence, which leads to significant yield losses. cotton can respond to withstand adverse environmental condition in several phases among which the accumulation of chem ... | 2016 | 27446165 |
| effect of waterlogging on carbohydrate metabolism and the quality of fiber in cotton (gossypium hirsutum l.). | transient waterlogging occurs frequently in the yangtze river and adversely affects cotton fiber quality. however, the carbohydrate metabolic mechanism that affects fiber quality after waterlogging remains undescribed. here, the effects of five waterlogging levels (0, 3, 6, 9, and 12 days) were assessed during flowering and boll formation to characterize the carbohydrates, enzymes and genes that affect the fiber quality of cotton after waterlogging. the cellulose and sucrose contents of cotton f ... | 2016 | 27446110 |
| stability of spatial distributions of stink bugs, boll injury, and ndvi in cotton. | a 3-yr study was conducted to determine the degree of aggregation of stink bugs and boll injury in cotton, gossypium hirsutum l., and their spatial association with a multispectral vegetation index (normalized difference vegetation index [ndvi]). using the spatial analysis by distance indices analyses, stink bugs were less frequently aggregated (17% for adults and 4% for nymphs) than boll injury (36%). ndvi values were also significantly aggregated within fields in 19 of 48 analyses (40%), with ... | 2016 | 27436843 |
| overexpression of ghsarp1 encoding a e3 ligase from cotton reduce the tolerance to salt in transgenic arabidopsis. | ubiquitination plays a very important role in the response to abiotic stresses of plant. to identify key regulators of salt stress, a gene ghsarp1(salt-associated ring finger protein)encoding c3h2c3-type e3 ligase, was cloned from cotton. transcription level of ghsarp1 was high in leaf, flower and fiber of 24,27 and 27dpa (days post-anthesis), but low in root and stem. except peg6000 treatment, the expression of ghsarp1 was down-regulated by nacl, cold and aba after being treated for 1 h. ghsarp ... | 2016 | 27402266 |
| mining and analysis of snp in response to salinity stress in upland cotton (gossypium hirsutum l.). | salinity stress is a major abiotic factor that affects crop output, and as a pioneer crop in saline and alkaline land, salt tolerance study of cotton is particularly important. in our experiment, four salt-tolerance varieties with different salt tolerance indexes including cri35 (65.04%), kanghuanwei164 (56.19%), zhong9807 (55.20%) and cri44 (50.50%), as well as four salt-sensitive cotton varieties including hengmian3 (48.21%), gk50 (40.20%), xinyan96-48 (34.90%), zhongs9612 (24.80%) were used a ... | 2016 | 27355327 |
| the cotton mitogen-activated protein kinase kinase 3 functions in drought tolerance by regulating stomatal responses and root growth. | mitogen-activated protein kinase (mapk) cascades play critical roles in signal transduction processes in eukaryotes. the mapk kinases (mapkks) that link mapkk kinases (mapkkks) and mapks are key components of mapk cascades. however, the intricate regulatory mechanisms that control mapkks under drought stress conditions are not fully understood, especially in cotton (gossypium hirsutum) here, we isolated and characterized the cotton group b mapkk gene ghmkk3 overexpressing ghmkk3 in nicotiana ben ... | 2016 | 27335349 |
| optimal cotton insecticide application termination timing: a meta-analysis. | the concept of insecticide termination timing is generally accepted among cotton (gossypium hirsutum) researchers; however, exact timings are often disputed. specifically, there is uncertainty regarding the last economic insecticide application to control fruit-feeding pests including tarnished plant bug (lygus lineolaris (palisot de beauvois)), boll weevil (anthonomus grandis), bollworm (helicoverpa zea), tobacco budworm (heliothis virescens), and cotton fleahopper (pseudatomoscelis seriatus). ... | 2016 | 27329633 |
| ssr-based association mapping of salt tolerance in cotton (gossypium hirsutum l.). | the identification of simple sequence repeat (ssr) markers associated with salt tolerance in cotton contributes to molecular assisted selection (mas), which can improve the efficiency of traditional breeding. in this study, 134 samples of upland cotton cultivars were selected. the seedling emergence rates were tested under 0.3% nacl stress. a total of 74 ssr markers were used to scan the genomes of these samples. to identify ssr markers associated with salt tolerance, an association analysis was ... | 2016 | 27323090 |
| trichomes control flower bud shape by linking together young petals. | trichomes are widespread in plants and develop from surface cells on different tissues(1). they have many forms and functions, from defensive spines to physical barriers that trap layers of air to insulate against desiccation, but there is growing evidence that trichomes can also have developmental roles in regulating flower structure(2,3). we report here that the trichomes on petals of cotton, gossypium hirsutum l., are essential for correct flower bud shape through a mechanical entanglement of ... | 2016 | 27322517 |
| cotton cytosolic pyruvate kinase ghpk6 participates in fast fiber elongation regulation in a ros-mediated manner. | cotton cytosolic pyruvate kinase ghpk6 is preferentially expressed in the late stage of fiber elongation process, transgenic experiments indicated that its expression level was negatively correlated to cell expansion rate. pyruvate kinase (pk) plays vital regulatory roles in rapid cell growth in mammals. however, the function of pk in plant cell growth remains unclear. in allotetraploid upland cotton (gossypium hirsutum l.), a total of 33 pk genes are encoded by the genome. analysis of the trans ... | 2016 | 27316434 |
| leaf ontogeny strongly influences photosynthetic tolerance to drought and high temperature in gossypium hirsutum. | temperature and drought are major abiotic limitations to crop productivity worldwide. while abiotic stress physiology research has focused primarily on fully expanded leaves, no studies have investigated photosynthetic tolerance to concurrent drought and high temperature during leaf ontogeny. to address this, gossypium hirsutum plants were exposed to five irrigation treatments, and two different leaf stages were sampled on three dates during an abnormally dry summer. early in the growing season, ... | 2016 | 27302003 |
| cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis. | cotton fibers arise through highly anisotropic expansion of a single seed epidermal cell. we obtained evidence that apical cell wall synthesis occurs through examining the tips of young elongating gossypium hirsutum (gh) and g. barbadense (gb) fibers. we characterized two tip types in gh fiber (hemisphere and tapered), each with distinct apical diameter, central vacuole location, and distribution of cell wall components. the apex of gh hemisphere tips was enriched in homogalacturonan epitopes, i ... | 2016 | 27301434 |
| genome-wide analysis of long noncoding rnas and their responses to drought stress in cotton (gossypium hirsutum l.). | recent researches on long noncoding rnas (lncrnas) have expanded our horizon of gene regulation and the cellular complexity. however, the number, characteristics and expression patterns of lncrnas remain poorly characterized and how these lncrnas biogenesis are regulated in response to drought stress in cotton are still largely unclear. in the study, using a reproducibility-based rna-sequencing and bioinformatics strategy to analyze the lncrnas of 9 samples under three different environment stre ... | 2016 | 27294517 |
| a cotton annexin affects fiber elongation and secondary cell wall biosynthesis associated with ca2+ influx, ros homeostasis, and actin filament reorganization. | annexins play pivotal roles in a variety of cellular processes as well as in fiber development; however, the functional mechanisms of their activities are unclear. here, an annexin gene that is preferentially expressed in fibers, ghfannxa, was found to be significantly associated with various cotton (gossypium hirsutum) fiber traits. transgenic analysis demonstrated that ghfannxa affected cotton fiber elongation and was involved in secondary cell wall (scw) biosynthesis. functional studies demon ... | 2016 | 27255486 |
| effects of soil salinity on sucrose metabolism in cotton leaves. | this study investigated sucrose metabolism of the youngest fully expanded main-stem leaf (msl) and the subtending leaf of cotton (gossypium hirsutum l.) boll (lscb) of salt-tolerant (ccri-79) and salt-sensitive (simian 3) cultivars and its relationship to boll weight under low, medium and high soil salinity stress in dafeng, china, in 2013 and 2014. the results showed that with increased soil salinity, 1) both the chlorophyll content and net photosynthetic rate (pn) decreased, while the internal ... | 2016 | 27228029 |
| genome-wide comparative analysis of the phospholipase d gene families among allotetraploid cotton and its diploid progenitors. | in this study, 40 phospholipase d (pld) genes were identified from allotetraploid cotton gossypium hirsutum, and 20 pld genes were examined in diploid cotton gossypium raimondii. combining with 19 previously identified gossypium arboreum pld genes, a comparative analysis was performed among the pld gene families among allotetraploid and two diploid cottons. based on the orthologous relationships, we found that almost each g. hirsutum pld had a corresponding homolog in the g. arboreum and g. raim ... | 2016 | 27213891 |
| development and validation of a fluorescent multiplexed immunoassay for measurement of transgenic proteins in cotton (gossypium hirsutum). | in order to provide farmers with better and more customized alternatives to improve yields, combining multiple genetically modified (gm) traits into a single product (called stacked trait crops) is becoming prevalent. trait protein expression levels are used to characterize new gm products and establish exposure limits, two important components of safety assessment. developing a multiplexed immunoassay capable of measuring all trait proteins in the same sample allows for higher sample throughput ... | 2016 | 27177195 |
| a genetic map between gossypium hirsutum and the brazilian endemic g. mustelinum and its application to qtl mapping. | among the seven tetraploid cotton species, little is known about transmission genetics and genome organization in gossypium mustelinum, the species most distant from the source of most cultivated cotton, g. hirsutum in this research, an f2 population was developed from an interspecific cross between g. hirsutum and g. mustelinum (hm). a genetic linkage map was constructed mainly using simple sequence repeat (ssrs) and restriction fragment length polymorphism (rflp) dna markers. the arrangements ... | 2016 | 27172208 |