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novosphingobium gossypii sp. nov., isolated from gossypium hirsutum.a gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain jm-1396(t)) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (gossypium hirsutum, cultivar 'des-119') grown at the plant breeding unit at the e.v. smith research center in tallassee (macon county), al, usa. 16s rrna gene sequence analysis of strain jm-1396(t) showed high sequence similarity values to the type strains of novosphingobium mathurense, novosphingobium panipatens ...201525985829
overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt.expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ...201525929795
genome-wide transcriptome analysis of cotton (gossypium hirsutum l.) identifies candidate gene signatures in response to aflatoxin producing fungus aspergillus flavus.aflatoxins are toxic and potent carcinogenic metabolites produced from the fungi aspergillus flavus and a. parasiticus. aflatoxins can contaminate cottonseed under conducive preharvest and postharvest conditions. united states federal regulations restrict the use of aflatoxin contaminated cottonseed at >20 ppb for animal feed. several strategies have been proposed for controlling aflatoxin contamination, and much success has been achieved by the application of an atoxigenic strain of a. flavus i ...201526366857
identification of mirnas and their targets in cotton inoculated with verticillium dahliae by high-throughput sequencing and degradome analysis.micrornas (mirnas) are a group of endogenous small non-coding rnas that play important roles in plant growth, development, and stress response processes. verticillium wilt is a vascular disease in plants mainly caused by verticillium dahliae kleb., the soil-borne fungal pathogen. however, the role of mirnas in the regulation of verticillium defense responses is mostly unknown. this study aimed to identify new mirnas and their potential targets that are involved in the regulation of verticillium ...201526133244
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid.abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ...201526587006
bacillus gossypii sp. nov., isolated from the stem of gossypium hirsutum.a gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of gossypium hirsutum, was studied to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity comparisons, strain jm-267t was grouped in the genus bacillus, related most closely to the type strains of bacillus simplex and bacillus huizhouensis (both 97.8%), bacillus muralis (97.7%), bacillus butanolivorans and bacillus psychrosaccharolyticus (both 97.3%). 16s rrna gene s ...201526303844
transformation and evaluation of cry1ac+cry2a and gtgene in gossypium hirsutum l.more than 50 countries around the globe cultivate cotton on a large scale. it is a major cash crop of pakistan and is considered "white gold" because it is highly important to the economy of pakistan. in addition to its importance, cotton cultivation faces several problems, such as insect pests, weeds, and viruses. in the past, insects have been controlled by insecticides, but this method caused a severe loss to the economy. however, conventional breeding methods have provided considerable break ...201526617613
fall armyworm (lepidoptera: noctuidae) development, survivorship, and damage on cotton plants expressing insecticidal plant-incorporated protectants.cotton, gossypium hirsutum (l.), plants expressing insecticidal crystal (cry) proteins of bacillus thuringiensis (bt) berliner are planted on significant acreage across the southern region of the united states. fall armyworm, spodoptera frugiperda (j. e. smith), can be a significant cotton pest in some years, but this species has not been a primary target of bt cotton technologies. the objective of this study was to quantify fall armyworm larval survivorship and fruiting form injury on transgeni ...201526470233
cadherin is involved in the action of bacillus thuringiensis toxins cry1ac and cry2aa in the beet armyworm, spodoptera exigua.bacillus thuringiensis (bt) insecticidal crystal (cry) proteins are effective against some insect pests in sprays and transgenic crops, although the evolution of resistance could threaten the long-term efficacy of such bt use. one strategy to delay resistance to bt crops is to "pyramid" two or more bt proteins that bind to distinct receptor proteins within the insect midgut. the most common bt pyramid in cotton (gossypium hirsutum l.) employs cry1ac with cry2ab to target several key lepidopteran ...201525754522
asiatic cotton can generate similar economic benefits to bt cotton under rainfed conditions.american cotton (gossypium hirsutum l.), transformed with bacillus thuringiensis cry genes (bt g. hirsutum) that confer resistance to lepidopteran pests, is extensively cultivated worldwide. in india, transgenic bt g. hirsutum was commercially released in 2002 and by 2014 95% of farmers had adopted bt g. hirsutum(1). the economic benefits of bt g. hirsutum over non-bt g. hirsutum are well documented and include increase in yields, increase in farmers' net revenue and reduction in pesticide appli ...201527250007
functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl burewala virus.cotton leaf curl burewala virus (clcubuv), belonging to the genus begomovirus, possesses single-stranded monopartite dna genome. the bidirectional promoters representing rep and coat protein (cp) genes of clcubuv were characterized and their efficacy was assayed. rep and cp promoters of clcubuv and 35s promoter of cauliflower mosaic virus (camv) were fused with β-glucuronidase (gus) and green fluorescent protein (gfp) reporter genes. gus activity in individual plant cells driven by rep, cp and 3 ...201525799504
high genetic homogeneity points to a single introduction event responsible for invasion of cotton leaf curl multan virus and its associated betasatellite into china.cotton leaf curl multan virus (clcumuv) is a whitefly transmitted geminivirus (wtg) endemic to the india subcontinent and is notorious as a causal agent of cotton leaf curl disease (clcud), a major constraint to cotton production in south asia. we found clcumuv infecting hibiscus rosa-sinensis in guangzhou, china in 2006. the spread and evolution of the invading clcumuv were monitored in the following nine years.201526445958
effect of high-temperature stress on the growth and seed characteristics of barley and cotton.the existence of a sensitivity gradient to a uniform high-temperature stress applied at stages during seed maturation can be demonstrated in barley. the germination of freshly harvested seed is depressed following heat stress at 7--10 days after awn emergence, but is enhanced by the same stress applied 3 weeks after awn emergence. the depression is attributed to reduced viability associated with thermal injury. the stimulation following stress at more mature stages of seed development is related ...20151032107
dose and time dependent efficacy alteration of different defoliants on seed cotton yield.field experiments were conducted during 2012 and 2013 to determine the effect of select defoliants i.e dropp ultra and ethrel, their optimal dose and suitable time of application on earliness and seed cotton yield in 3 american cotton cultivars. mrc7361bgii (3055.2 kg ha(-1)) and mrc7017bgii (2913.0 kg ha(-1)) resulted in significantly improved seed cotton yield as compared to f1 861 (2346.0 kg ha(-1)). dropp ultra? @ 200 ml ha(-1) revealed highest yield (3018.7 kg ha(-1)) followed by ethrel @ 2 ...201526364466
evaluation of different carbon sources for high frequency callus culture with reduced phenolic secretion in cotton (gossypium hirsutum l.) cv. svpr-2.an efficient protocol was developed to control excessive phenolic compound secretion during callus culture of cotton. as cotton is naturally rich in phenolic compounds factors influencing the phenolic compound secretion, callus induction and proliferation were optimized for getting high frequency callus culture. different carbon sources such as fructose, glucose, sucrose and maltose were tested at various concentrations to control phenolic secretion in callus culture. among them, 3% maltose was ...201528626717
[disturbed structure and function of chloroplasts during blocked biosynthesis of 5'-aminolevulinic acid in the light].xantha-702 mutant of cotton (gossypium hirsutum l.) proved to have blocked synthesis of 5'-aminolevulinic acid in the light. accordingly, mutant leaves accumulated 2-5% chlorophyll of baseline. mutant plants demonstrated disturbed production of pigment-protein complexes of photosystems i (psi) and ii (psii) and generation of the chloroplast membrane system blocked at the early stages, largely, at the stages of vesicles and single short thylakoid. the functional activity of the psi and psii react ...201617853692
comparative analysis of resistance gene analogues encoding nbs-lrr domains in cotton.plant production is severely affected by biotic and abiotic stresses r-genes exhibit resistance against a range of diseases and pathogens in plants. the nucleotide binding site and leucine rich repeat (nbs-lrr) class of r-genes is the most comprehensively studied in terms of sequence evolution and genome distribution. the differential response for resistance against biotic and abiotic stress has been observed in cultivated and wild relatives of the genus gossypium.201625640313
cotton square morphology offers new insights into host plant resistance to cotton fleahopper (hemiptera: miridae) in upland cotton.cotton fleahopper, pseudatomoscelis seriatus (reuter) (hemiptera: miridae), is a piercing-sucking pest of cotton (gossypium hirsutum l.) that feeds preferentially on developing flower buds, called squares. heavy infestations cause yield reductions that result from abscission of squares damaged by the cotton fleahopper feeding. antixenosis, or nonpreference, has been reported as a mechanism of host plant resistance in cotton to cotton fleahopper. square structure, particularly the placement of th ...201626475922
ghnac12, a neutral candidate gene, leads to early aging in cotton (gossypium hirsutum l).nac (nam, ataf, and cuc) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. currently, 77 nac genes have been reported in cotton (gossypium hirsutum l.). and ghnac12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. in the present study, a preliminary function analysis of ghnac12 was performed during leaf senescence. qrt-pcr a ...201626496006
effect of the postfeeding interval on olfactory responses of thrips to herbivore-induced cotton plants.we investigated the responses of 3 thrips species, frankliniella schultzei trybom, f. occidentalis pergrande, and thrips tabaci lindeman (thysanoptera: thripidae) to herbivore-damaged and undamaged cotton seedlings (gossypium hirsutum l. [malvales: malvaceae]) at a range of time intervals following damage by adult tetranychus urticae (koch), adult t. ludeni (zacher) (acari: tetranychidae) or helicoverpa armigera (hübner) (lepidoptera: noctuidae) larvae in olfactometer assays. the intensity/frequ ...201626172119
arabidopsis edt1/hdg11 improves drought and salt tolerance in cotton and poplar and increases cotton yield in the field.drought and salinity are two major environmental factors limiting crop production worldwide. improvement of drought and salt tolerance of crops with transgenic approach is an effective strategy to meet the demand of the ever-growing world population. arabidopsis enhanced drought tolerance1/homeodomain glabrous11 (atedt1/hdg11), a homeodomain-start transcription factor, has been demonstrated to significantly improve drought tolerance in arabidopsis, tobacco, tall fescue and rice. here we report t ...201625879154
lightweight males of podisus nigrispinus (heteroptera: pentatomidae) neglect lightweight females due low reproductive fitness.sexual choice by male stink bugs is important because females that experience food shortages lay fewer eggs with lower viability compared with well-fed females. in this study, we investigated whether podisus nigrispinus (dallas) (heteroptera: pentatomidae) males fed with a low-quality diet during its nymphal stage show selectivity for sexual partners resulting in high-quality progeny. lightweight males and females were obtained from nymphs fed weekly with tenebrio molitor l. (coleoptera: tenebri ...201627599098
monopodial and sympodial branching architecture in cotton is differentially regulated by the gossypium hirsutum single flower truss and self-pruning orthologs.domestication of upland cotton (gossypium hirsutum) converted it from a lanky photoperiodic perennial to a day-neutral annual row-crop. residual perennial traits, however, complicate irrigation and crop management, and more determinate architectures are desired. cotton simultaneously maintains robust monopodial indeterminate shoots and sympodial determinate shoots. we questioned if and how the flowering locus t/single flower truss (sft)-like and terminal flower1/self-pruning (sp)-like genes cont ...201627292411
resistance to glufosinate is proportional to phosphinothricin acetyltransferase expression and activity in libertylink(®) and widestrike(®) cotton.insertion of the gene encoding phosphinothricin acetyltransferase (pat) has resulted in cotton plants resistant to the herbicide glufosinate. however, the lower expression and commensurate reduction in pat activity is a key factor in the low level of injury observed in the widestrike(®) cotton and relatively high level of resistance observed in libertylink(®) cotton. libertylink(®) cotton cultivars are engineered for glufosinate resistance by overexpressing the bar gene that encodes phosphinothr ...201626733464
frankliniella fusca resistance to neonicotinoid insecticides: an emerging challenge for cotton pest management in the eastern united states.over the past two decades, neonicotinoid seed treatments have become the primary method to manage tobacco thrips, frankliniella fusca hinds, on seedling cotton. because this insect is highly polyphagous and the window of insecticide exposure is short, neonicotinoid resistance was expected to pose a minimal risk. however, reports of higher than expected f. fusca seedling damage in seed-treated cotton fields throughout the mid-south and southeast us production regions suggested neonicotinoid resis ...201626775623
relative fitness of helicoverpa armigera (lepidoptera: noctuidae) on seven host plants: a perspective for ipm in brazil.the cotton bollworm helicoverpa armigera (hübner) (lepidoptera: noctuidae) is a widespread pest of many cultivated and wild plants in europe, africa, asia, and australia. in 2013, this species was reported in brazil, attacking various host crops in the midwestern and northeastern regions of the country and is now found countrywide. aiming to understand the effects of different host plants on the life cycle of h. armigera, we selected seven species of host plants that mature in different seasons ...201626798139
partial life history of chrysodeixis includens (lepidoptera: noctuidae) on summer hosts.the soybean looper, chrysodeixis includens (walker) (lepidoptera: noctuidae), is a major defoliating pest of soybeans, glycine max (l.) merrill, in louisiana. however, other alternate host crops in the agroecosystem have the potential to impact c. includens populations. life table statistics of c. includens on four host plants were evaluated. c. includens larvae were fed leaves of three cotton gossypium hirsutum l. cultivars 'dp 143 b2rf,' 'dp 174 rf,' and 'phy 485 wrf'; cowpea vigna unguiculata ...201627375294
evolution of gene duplication in plants.ancient duplication events and a high rate of retention of extant pairs of duplicate genes have contributed to an abundance of duplicate genes in plant genomes. these duplicates have contributed to the evolution of novel functions, such as the production of floral structures, induction of disease resistance, and adaptation to stress. additionally, recent whole-genome duplications that have occurred in the lineages of several domesticated crop species, including wheat (triticum aestivum), cotton ...201627288366
gbexpatr, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring.cotton provides us the most important natural fibre. high fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from gossypium barbadense to gossypium hirsutum is an important breeding strategy. we previously analysed the g. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from g. barbadense, gbexpa2 and gbexpatr. gbexpa2 (from the dt genome) is a classical α-e ...201626269378
quantitative trait loci analysis of verticillium wilt resistance in interspecific backcross populations of gossypium hirsutum × gossypium barbadense.verticillium wilt (vw) caused by verticillium dahliae (kleb) is one of the most destructive diseases of cotton. the identification of highly resistant qtls or genes in the whole cotton genome is quite important for developing a vw-resistant variety and for further molecular design breeding.201627814678
spatio-temporal, genotypic, and environmental effects on plant soluble protein and digestible carbohydrate content: implications for insect herbivores with cotton as an exemplar.plant soluble protein and digestible carbohydrate content significantly affect insect herbivore fitness, but studies reporting plant protein and carbohydrate content are rare. instead, the elements nitrogen and carbon often are used as surrogates for plant protein and digestible carbohydrate content, respectively. however, this is problematic for two reasons. first, carbon is found in all organic molecules, which precludes strong correlations with ecologically important dietary macronutrients (e ...201627738861
identification of chromosome segment substitution lines of gossypium barbadense introgressed in g. hirsutum and quantitative trait locus mapping for fiber quality and yield traits.chromosome segment substitution lines mbi9804, mbi9855, mbi9752, and mbi9134, which were obtained by advanced backcrossing and continuously inbreeding from an interspecific cross between ccri36, a cultivar of upland cotton (gossypium hirsutum) as the recurrent parent, and hai1, a cultivar of sea island cotton (g. barbadense) as the donor parent, were used to construct a multiple parent population of (mbi9804×mbi9855)×(mbi9752×mbi9134). the segregating generations of double-crossed f1 and f2 and ...201627603312
genetic effects and heterosis of yield and yield component traits based on gossypium barbadense chromosome segment substitution lines in two gossypium hirsutum backgrounds.we hybridized 10 chromosome segment substitution lines (cssls) each from two cssl populations and produced 50 f1 hybrids according to north carolina design ii. we analyzed the genetic effects and heterosis of yield and yield components in the f1 hybrids and parents in four environments via the additive-dominance genetic model. yield and yield components of the cssls were controlled by combined additive and dominance effects, and lint percentage was mainly controlled by additive effects, but boll ...201627348815
independent domestication of two old world cotton species.domesticated cotton species provide raw material for the majority of the world's textile industry. two independent domestication events have been identified in allopolyploid cotton, one in upland cotton (gossypium hirsutum l.) and the other to egyptian cotton (gossypium barbadense l.). however, two diploid cotton species, gossypium arboreum l. and gossypium herbaceum l., have been cultivated for several millennia, but their status as independent domesticates has long been in question. using geno ...201627289095
a comparative transcriptome analysis of two sets of backcross inbred lines differing in lint-yield derived from a gossypium hirsutum × gossypium barbadense population.upland cotton (gossypium hirsutum l.) is the most important fiber crop, and its lint-yield improvement is impeded due to its narrow genetic base and the lack of understanding of the genetic basis of yield. backcross inbred lines (bils) or near-isogenic lines (nils) in the same genetic background differing in lint yield, developed through advanced backcrossing, provide an important genomic resource to study the molecular genetic basis of lint yield. in the present study, a high-yield (hy) group a ...201627256327
to be a flower or fruiting branch: insights revealed by mrna and small rna transcriptomes from different cotton developmental stages.the architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. nulliplex branch is a useful phenotype to study cotton architecture. we used rna sequencing to obtain mrna and mirna profiles from nulliplex- and normal-branch cotton at three developmental stages. the differentially expressed genes (degs) and mirnas were identified that preferentially/specifica ...201626983497
genetic diversity of the two commercial tetraploid cotton species in the gossypium diversity reference set.a diversity reference set has been constructed for the gossypium accessions in the us national cotton germplasm collection to facilitate more extensive evaluation and utilization of accessions held in the collection. a set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid gossypium accessions representative of the range of diversity of the improved and wild accessions of g. hirsutum and g. barbadense. the reference set contained 410 g. barbad ...201626774060
breeding potential of introgression lines developed from interspecific crossing between upland cotton (gossypium hirsutum) and gossypium barbadense: heterosis, combining ability and genetic effects.upland cotton (gossypium hirstum l.), which produces more than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. introducing germplasm from exotic sources especially from another cultivated tetraploid g. barbadense l. can broaden the genetic base of upland cotton. however, the breeding potential of introgression lines (ils) in upland cotton with g. barbadense germplasm integration has not been well addressed. this study involved six ils ...201626730964
transposable elements play an important role during cotton genome evolution and fiber cell development.transposable elements (tes) usually occupy largest fractions of plant genome and are also the most variable part of the structure. although traditionally it is hallmarked as "junk and selfish dna", today more and more evidence points out te's participation in gene regulations including gene mutation, duplication, movement and novel gene creation via genetic and epigenetic mechanisms. the recently sequenced genomes of diploid cottons gossypium arboreum (aa) and gossypium raimondii (dd) together w ...201626687725
chlorophyll fluorescence induction kinetics and yield responses in rainfed crops with variable potassium nutrition in k deficient semi-arid alfisols.optimum potassium (k) nutrition in semi-arid regions may help crop plants to overcome constraints in their growth and development such as moisture stress, leading to higher productivity of rainfed crops, thus judicious k management is essential. a study was conducted to evaluate the importance of k nutrition on physiological processes like photosynthesis through chlorophyll a fluorescence and chlorophyll fluorescence induction kinetics (ojip) of rainfed crops viz., maize (zea mays l.), pearl mil ...201627101276
identification and evidence of positive selection upon resistance gene analogs in cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is an important fiber crop species, which is intensively plagued by a plethora of phytopathogenic fungi such as fusarium oxysporum f. sp. vasinfectum (fov) causing severe wilt disease. resistance gene analogs (rgas) are the largest class of potential resistance (r) genes depicting highly conserved domains and structures in plants. additionally, rgas are pivotal components of breeding projects towards host disease resistance, serving as useful functional mark ...201627729728
effects of silicon treatment and inoculation with fusarium oxysporum f. sp. vasinfectum on cellular defences in root tissues of two cotton cultivars.silicon has been shown to enhance the resistance of plants to fungal and bacterial pathogens. here, the effect of potassium silicate was assessed on two cotton (gossypium hirsutum) cultivars subsequently inoculated with fusarium oxysporum f. sp. vasinfectum (fov). sicot 189 is moderately resistant whilst sicot f-1 is the second most resistant commercial cultivar presently available in australia.201627288509
coupling of mic-3 overexpression with the chromosomes 11 and 14 root-knot nematode (rkn) (meloidogyne incognita) resistance qtls provides insights into the regulation of the rkn resistance response in upland cotton (gossypium hirsutum).genetic analysis of mic-3 transgene with rkn resistance qtls provides insight into the resistance regulatory mechanism and provides a framework for testing additional hypotheses. resistance to root-knot nematode (rkn) (meloidogyne incognita) in upland cotton (gossypium hirsutum) is mediated by two major quantitative trait loci (qtl) located on chromosomes 11 and 14. the mic-3 (meloidogyne induced cotton3) protein accumulates specifically within the immature galls of rkn-resistant plants that pos ...201627314265
identification and characterization of micrornas in the plant parasitic root-knot nematode meloidogyne incognita using deep sequencing.the root-knot nematode meloidogyne incognita is among the most damaging plant-parasitic pests of several crops including cotton (gossypium hirsutum) and tomato (lycopersicon escultentum). recently, a genome has become available for m. incognita, which greatly facilitates investigation of the interactions between m. incognita and its plant hosts at the molecular level and enables formation of hypotheses concerning development at the cellular level. micrornas (mirnas) are a class of small rna mole ...201626743520
cotton (gossypium hirsutum l.) boll rotting bacteria vectored by the brown stink bug, euschistus servus (say) (hemiptera: pentatomidae).to determine the capacity of the brown stink bug (bsb) (euschistus servus) to transmit an infective pantoea agglomerans strain sc 1-r into cotton (gossypium hirsutum) bolls.201627261673
effects of single and dual species herbivory on the behavioural responses of three thrips species to cotton seedlings.this study investigated the olfactory responses of three thrips species [frankliniella schultzei trybom, f. occidentalis pergrande and thrips tabaci lindeman (thysanoptera: thripidae)] to cotton seedlings [gossypium hirsutum l. (malvales: malvaceae)] simultaneously damaged by different combinations of herbivores. cotton seedlings were damaged by foliar feeding tetranychus urticae koch (trombidiforms: tetranychidae), helicoverpa armigera hübner (lepidoptera: noctuidae), aphis gossypii glover (hem ...201627029603
gherf-iib3 regulates the accumulation of jasmonate and leads to enhanced cotton resistance to blight disease.the phytohormone jasmonic acid (ja) and its derivatives, collectively referred to as jasmonates, regulate many developmental processes, but are also involved in the response to numerous abiotic/biotic stresses. thus far, powerful reverse genetic strategies employing perception, signalling or biosynthesis mutants have broadly contributed to our understanding of the role of ja in the plant stress response and development, as has the chemical gain-of-function approach based on exogenous application ...201627291786
fatty acid and proximate composition of bee bread.palynological spectrum, proximate and fatty acid (fa) composition of eight bee bread samples of different botanical origins were examined and significant variations were observed. the samples were all identified as monofloral, namely castanea sativa (94.4%), trifolium spp. (85.6%), gossypium hirsutum (66.2%), citrus spp. (61.4%) and helianthus annuus (45.4%). each had moisture content between 11.4 and 15.9%, ash between 1.9 and 2.54%, fat between 5.9 and 11.5%, and protein between 14.8 and 24.3% ...201628115909
genome-wide identification and functional analysis of the tify gene family in response to drought in cotton.jasmonates control many aspects of plant biological processes. they are important for regulating plant responses to various biotic and abiotic stresses, including drought, which is one of the most serious threats to sustainable agricultural production. however, little is known regarding how jasmonate zim-domain (jaz) proteins mediate jasmonic acid signals to improve stress tolerance in cotton. this represents the first comprehensive comparative study of tify transcription factors in both diploid ...201627640194
insights into interspecific hybridization events in allotetraploid cotton formation from characterization of a gene-regulating leaf shape.the morphology of cotton leaves varies considerably. phenotypes, including okra, sea-island, super-okra, and broad leaf, are controlled by a multiple allele locus, l2 okra leaf (l2°) is an incomplete mutation that alters leaf shape by increasing the length of lobes with deeper sinuses. using a map-based cloning strategy, we cloned the l2 locus gene, which encodes a late meristem identity 1 (lmi1)-like transcription factor (ghokra). silencing ghokra leads to a change in phenotype from okra to bro ...201627558139
functional characterization of a basic helix-loop-helix (bhlh) transcription factor ghdel65 from cotton (gossypium hirsutum).cotton fiber is proposed to share some similarity with the arabidopsis thaliana leaf trichome, which is regulated by the myb-bhlh-wd40 transcription complex. although several myb transcription factors and wd40 family proteins in cotton have been characterized, little is known about the role of bhlh family proteins in cotton. here, we report that ghdel65, a bhlh protein from cotton (gossypium hirsutum), is a functional homologue of arabidopsis glabra3 (gl3) and enhancer of glabra3 (egl3) in regul ...201627080593
promoter isolation and characterization of ghao-like1, a gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs.cotton is one of the most economically important cultivated crops. it is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. the isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified cro ...201626692462
gossypium arboreum accessions resistant to rotylenchulus reniformis.in the southeastern united states, reniform nematode (rotylenchulus reniformis) is a serious pest of upland cotton (gossypium hirsutum), a species which has no naturally occurring resistance against this nematode. to identify sources of reniform nematode resistance in species closely related to upland cotton, 222 g. arboreum accessions from the u.s. germplasm collection were evaluated in repeated growth chamber experiments. in initial screenings, root infection was measured 4 wks after inoculati ...201628154428
saccharibacillus endophyticus sp. nov., an endophyte of cotton.a gram-positive-staining, facultatively aerobic, endospore-forming bacterial strain, isolated from the stem tissue of cotton (gossypium hirsutum) was subjected to a detailed taxonomic study using a polyphasic approach. based on 16s rrna gene sequence similarity comparisons, strain jm-1350t grouped into the genus saccharibacillus, and was most closely related to the type strain of saccharibacillus sacchari (96.5 % 16s rrna gene sequence similarity), followed by the type strains of saccharibacillu ...201627613514
virus-induced gene silencing in cultivated cotton (gossypium spp.) using tobacco rattle virus.the study described here has optimized the conditions for virus-induced gene silencing (vigs) in three cultivated cotton species (gossypium hirsutum, g. arboreum, and g. herbaceum) using a tobacco rattle virus (trv) vector. the system was used to silence the homolog of the arabidopsis thaliana chloroplastos alterados 1 (atcla1) gene, involved in chloroplast development, in g. herbaceum, g. arboreum, and six commercial g. hirsutum cultivars. all plants inoculated with the trv vector to silence cl ...201626603442
functional divergence of ghcfe5 homoeologs revealed in cotton fiber and arabidopsis root cell development.in ghcfe5 homoeologs, ghcfe5d interacted with more actin homologs and stronger interaction activity than ghcfe5a. ghcfe5d - but not ghcfe5a -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. homoeologous genes are common in polyploid plants; however, their functional divergence is poorly elucidated. allotetraploid upland cotton (gossypium hirsutum, aadd) is the most widely cultivated cotton; accounting for more than 90 % of the world' ...201626759310
functional characterization of ghsoc1 and ghmads42 homologs from upland cotton (gossypium hirsutum l.).in arabidopsis flowering pathway, mads-box genes encode transcription factors, with their structures and functions highly conserved in many species. in our study, two mads-box genes ghsoc1 and ghmads42 (gossypium hirsutum l.) were cloned from upland cotton ccri36 and transformed into arabidopsis. ghsoc1 was additionally transformed into upland cotton. comparative analysis demonstrated sequence conservation between ghsoc1 and ghmads42 and genes of other plant species. tissue-specific expression a ...201626566835
genome-scale analysis of the cotton kcs gene family revealed a binary mode of action for gibberellin a regulated fiber growth.production of β-ketoacyl-coa, which is catalyzed by 3-ketoacyl-coa synthase (kcs), is the first step in very long chain fatty acid (vlcfa) biosynthesis. here we identified 58 kcs genes from gossypium hirsutum, 31 from g. arboreum and 33 from g. raimondii by searching the assembled cotton genomes. the gene family was divided into the plant-specific fae1-type and the more general elo-type. kcs transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or ...201626399709
physiological performance and differential expression profiling of genes associated with drought tolerance in root tissue of four contrasting varieties of two gossypium species.root growth in drying soil is generally limited by a combination of mechanical impedance and water stress. as the major function of root tissue is water and nutrient uptake, so it imparts an important role in plant growth and stress management. previously, we have studied physiological performance and expression profiling of gene associated with drought tolerance in leaf tissue of four cotton varieties. here, we have further continued our studies with the root tissue of these varieties. the goss ...201625802007
metabolic engineering of cottonseed oil biosynthesis pathway via rna interference.cottonseed oil is recognized as an important oil in food industry for its unique characters: low flavor reversion and the high level of antioxidants (vitamine) as well as unsaturated fatty acid. however, the cottonseed oil content of cultivated cotton (gossypium hirsutum) is only around 20%. in this study, we modified the accumulation of oils by the down-regulation of phosphoenolpyruvate carboxylase 1 (ghpepc1) via rna interference in transgenic cotton plants. the qrt-pcr and enzyme activity ass ...201627620452
genome-wide characterization and expression analysis of myb transcription factors in gossypium hirsutum.myb family proteins are one of the most abundant transcription factors in the cotton plant and play diverse roles in cotton growth and evolution. previously, few studies have been conducted in upland cotton, gossypium hirsutum. the recent release of the g. hirsutum genome sequence provides a great opportunity to identify and characterize the entire upland cotton myb protein family.201627613381
cloning and functional analysis of the promoter of an ascorbate oxidase gene from gossypium hirsutum.apoplastic ascorbate oxidase (ao) plays significant roles in plant cell growth. however, the mechanism of underlying the transcriptional regulation of ao in gossypium hirsutum remains unclear. here, we obtained a 1,920-bp promoter sequence from the gossypium hirsutum ascorbate oxidase (ghao1) gene, and this ghao1 promoter included a number of known cis-elements. promoter activity analysis in overexpressing pghao1::gfp-gus tobacco (nicotiana benthamiana) showed that the ghao1 promoter exhibited h ...201627597995
identification and characterization of the ghhsp20 gene family in gossypium hirsutum.in higher plants, heat shock protein 20 (hsp20) plays crucial roles in growth, development and responses to abiotic stresses. in this study, 94 ghhsp20 genes were identified in g. hirsutum, and these genes were phylogenetically clustered into 14 subfamilies. out of these, 73 paralogous gene pairs remained in conserved positions on segmental duplicated blocks and only 14 genes clustered into seven tandem duplication event regions. transcriptome analysis showed that 82 ghhsp20 genes were expressed ...201627580529
identification of favorable snp alleles and candidate genes for traits related to early maturity via gwas in upland cotton.early maturity is one of the most important and complex agronomic traits in upland cotton (gossypium hirsutum l). to dissect the genetic architecture of this agronomically important trait, a population consisting of 355 upland cotton germplasm accessions was genotyped using the specific-locus amplified fragment sequencing (slaf-seq) approach, of which a subset of 185 lines representative of the diversity among the accessions was phenotypically characterized for six early maturity traits in four ...201627576450
the ghtt2_a07 gene is linked to the brown colour and natural flame retardancy phenotypes of lc1 cotton (gossypium hirsutum l.) fibres.some naturally coloured brown cotton fibres from accessions of gossypium hirsutum l. can be used to make textiles with enhanced flame retardancy (fr). several independent brown fibre loci have been identified and mapped to chromosomes, but the underlying genes have not yet been identified, and the mechanism of lint fibre fr is not yet fully understood. in this study, we show that both the brown colour and enhanced fr of the lc1 lint colour locus are linked to a 1.4mb inversion on chromosome a07 ...201627567364
characterization and functional analysis of pebp family genes in upland cotton (gossypium hirsutum l.).upland cotton (gossypium hirsutum l.) is a naturally occurring photoperiod-sensitive perennial plant species. however, sensitivity to the day length was lost during domestication. the phosphatidylethanolamine-binding protein (pebp) gene family, of which three subclades have been identified in angiosperms, functions to promote and suppress flowering in photoperiod pathway. recent evidence indicates that pebp family genes play an important role in generating mobile flowering signals. we isolated h ...201627552108
mapping qtls for drought tolerance in an f2:3 population from an inter-specific cross between gossypium tomentosum and gossypium hirsutum.cotton is one of the most important natural fiber crops in the world. its growth and yield is greatly limited by drought. a quantitative trait locus (qtl) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (gossypium spp) using 188 f2:3 lines developed from an inter-specific cross between a wild cotton species, g. tomentosum, and an upland cotton, g. hirsutum (cri-12). a genetic map was constructed using 1295 simple sequence repeat markers, which amp ...201627525919
de novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (g. hirsutum l.).plant regeneration via somatic embryogenesis (se) is the key step for genetic improvement of cotton (gossypium hirsutum l.) through genetic engineering mediated by agrobacteria, but the molecular mechanisms underlying se in cotton is still unclear. here, rna-sequencing was used to analyze the genes expressed during se and their expression dynamics using rnas isolated from non-embryogenic callus (nec), embryogenic callus (ec) and somatic embryos (ses). a total of 101, 670 unigenes were de novo as ...201627511192
response and tolerance mechanism of cotton gossypium hirsutum l. to elevated temperature stress: a review.cotton is an important multipurpose crop which is highly sensitive to both biotic and abiotic stresses. proper management of this cash crop requires systematic understanding of various environmental conditions that are vital to yield and quality. high temperature stress can severely affect the viability of pollens and anther indehiscence, which leads to significant yield losses. cotton can respond to withstand adverse environmental condition in several phases among which the accumulation of chem ...201627446165
effect of waterlogging on carbohydrate metabolism and the quality of fiber in cotton (gossypium hirsutum l.).transient waterlogging occurs frequently in the yangtze river and adversely affects cotton fiber quality. however, the carbohydrate metabolic mechanism that affects fiber quality after waterlogging remains undescribed. here, the effects of five waterlogging levels (0, 3, 6, 9, and 12 days) were assessed during flowering and boll formation to characterize the carbohydrates, enzymes and genes that affect the fiber quality of cotton after waterlogging. the cellulose and sucrose contents of cotton f ...201627446110
stability of spatial distributions of stink bugs, boll injury, and ndvi in cotton.a 3-yr study was conducted to determine the degree of aggregation of stink bugs and boll injury in cotton, gossypium hirsutum l., and their spatial association with a multispectral vegetation index (normalized difference vegetation index [ndvi]). using the spatial analysis by distance indices analyses, stink bugs were less frequently aggregated (17% for adults and 4% for nymphs) than boll injury (36%). ndvi values were also significantly aggregated within fields in 19 of 48 analyses (40%), with ...201627436843
overexpression of ghsarp1 encoding a e3 ligase from cotton reduce the tolerance to salt in transgenic arabidopsis.ubiquitination plays a very important role in the response to abiotic stresses of plant. to identify key regulators of salt stress, a gene ghsarp1(salt-associated ring finger protein)encoding c3h2c3-type e3 ligase, was cloned from cotton. transcription level of ghsarp1 was high in leaf, flower and fiber of 24,27 and 27dpa (days post-anthesis), but low in root and stem. except peg6000 treatment, the expression of ghsarp1 was down-regulated by nacl, cold and aba after being treated for 1 h. ghsarp ...201627402266
mining and analysis of snp in response to salinity stress in upland cotton (gossypium hirsutum l.).salinity stress is a major abiotic factor that affects crop output, and as a pioneer crop in saline and alkaline land, salt tolerance study of cotton is particularly important. in our experiment, four salt-tolerance varieties with different salt tolerance indexes including cri35 (65.04%), kanghuanwei164 (56.19%), zhong9807 (55.20%) and cri44 (50.50%), as well as four salt-sensitive cotton varieties including hengmian3 (48.21%), gk50 (40.20%), xinyan96-48 (34.90%), zhongs9612 (24.80%) were used a ...201627355327
the cotton mitogen-activated protein kinase kinase 3 functions in drought tolerance by regulating stomatal responses and root growth.mitogen-activated protein kinase (mapk) cascades play critical roles in signal transduction processes in eukaryotes. the mapk kinases (mapkks) that link mapkk kinases (mapkkks) and mapks are key components of mapk cascades. however, the intricate regulatory mechanisms that control mapkks under drought stress conditions are not fully understood, especially in cotton (gossypium hirsutum) here, we isolated and characterized the cotton group b mapkk gene ghmkk3 overexpressing ghmkk3 in nicotiana ben ...201627335349
optimal cotton insecticide application termination timing: a meta-analysis.the concept of insecticide termination timing is generally accepted among cotton (gossypium hirsutum) researchers; however, exact timings are often disputed. specifically, there is uncertainty regarding the last economic insecticide application to control fruit-feeding pests including tarnished plant bug (lygus lineolaris (palisot de beauvois)), boll weevil (anthonomus grandis), bollworm (helicoverpa zea), tobacco budworm (heliothis virescens), and cotton fleahopper (pseudatomoscelis seriatus). ...201627329633
ssr-based association mapping of salt tolerance in cotton (gossypium hirsutum l.).the identification of simple sequence repeat (ssr) markers associated with salt tolerance in cotton contributes to molecular assisted selection (mas), which can improve the efficiency of traditional breeding. in this study, 134 samples of upland cotton cultivars were selected. the seedling emergence rates were tested under 0.3% nacl stress. a total of 74 ssr markers were used to scan the genomes of these samples. to identify ssr markers associated with salt tolerance, an association analysis was ...201627323090
trichomes control flower bud shape by linking together young petals.trichomes are widespread in plants and develop from surface cells on different tissues(1). they have many forms and functions, from defensive spines to physical barriers that trap layers of air to insulate against desiccation, but there is growing evidence that trichomes can also have developmental roles in regulating flower structure(2,3). we report here that the trichomes on petals of cotton, gossypium hirsutum l., are essential for correct flower bud shape through a mechanical entanglement of ...201627322517
cotton cytosolic pyruvate kinase ghpk6 participates in fast fiber elongation regulation in a ros-mediated manner.cotton cytosolic pyruvate kinase ghpk6 is preferentially expressed in the late stage of fiber elongation process, transgenic experiments indicated that its expression level was negatively correlated to cell expansion rate. pyruvate kinase (pk) plays vital regulatory roles in rapid cell growth in mammals. however, the function of pk in plant cell growth remains unclear. in allotetraploid upland cotton (gossypium hirsutum l.), a total of 33 pk genes are encoded by the genome. analysis of the trans ...201627316434
leaf ontogeny strongly influences photosynthetic tolerance to drought and high temperature in gossypium hirsutum.temperature and drought are major abiotic limitations to crop productivity worldwide. while abiotic stress physiology research has focused primarily on fully expanded leaves, no studies have investigated photosynthetic tolerance to concurrent drought and high temperature during leaf ontogeny. to address this, gossypium hirsutum plants were exposed to five irrigation treatments, and two different leaf stages were sampled on three dates during an abnormally dry summer. early in the growing season, ...201627302003
cotton fiber tips have diverse morphologies and show evidence of apical cell wall synthesis.cotton fibers arise through highly anisotropic expansion of a single seed epidermal cell. we obtained evidence that apical cell wall synthesis occurs through examining the tips of young elongating gossypium hirsutum (gh) and g. barbadense (gb) fibers. we characterized two tip types in gh fiber (hemisphere and tapered), each with distinct apical diameter, central vacuole location, and distribution of cell wall components. the apex of gh hemisphere tips was enriched in homogalacturonan epitopes, i ...201627301434
genome-wide analysis of long noncoding rnas and their responses to drought stress in cotton (gossypium hirsutum l.).recent researches on long noncoding rnas (lncrnas) have expanded our horizon of gene regulation and the cellular complexity. however, the number, characteristics and expression patterns of lncrnas remain poorly characterized and how these lncrnas biogenesis are regulated in response to drought stress in cotton are still largely unclear. in the study, using a reproducibility-based rna-sequencing and bioinformatics strategy to analyze the lncrnas of 9 samples under three different environment stre ...201627294517
a cotton annexin affects fiber elongation and secondary cell wall biosynthesis associated with ca2+ influx, ros homeostasis, and actin filament reorganization.annexins play pivotal roles in a variety of cellular processes as well as in fiber development; however, the functional mechanisms of their activities are unclear. here, an annexin gene that is preferentially expressed in fibers, ghfannxa, was found to be significantly associated with various cotton (gossypium hirsutum) fiber traits. transgenic analysis demonstrated that ghfannxa affected cotton fiber elongation and was involved in secondary cell wall (scw) biosynthesis. functional studies demon ...201627255486
effects of soil salinity on sucrose metabolism in cotton leaves.this study investigated sucrose metabolism of the youngest fully expanded main-stem leaf (msl) and the subtending leaf of cotton (gossypium hirsutum l.) boll (lscb) of salt-tolerant (ccri-79) and salt-sensitive (simian 3) cultivars and its relationship to boll weight under low, medium and high soil salinity stress in dafeng, china, in 2013 and 2014. the results showed that with increased soil salinity, 1) both the chlorophyll content and net photosynthetic rate (pn) decreased, while the internal ...201627228029
genome-wide comparative analysis of the phospholipase d gene families among allotetraploid cotton and its diploid progenitors.in this study, 40 phospholipase d (pld) genes were identified from allotetraploid cotton gossypium hirsutum, and 20 pld genes were examined in diploid cotton gossypium raimondii. combining with 19 previously identified gossypium arboreum pld genes, a comparative analysis was performed among the pld gene families among allotetraploid and two diploid cottons. based on the orthologous relationships, we found that almost each g. hirsutum pld had a corresponding homolog in the g. arboreum and g. raim ...201627213891
development and validation of a fluorescent multiplexed immunoassay for measurement of transgenic proteins in cotton (gossypium hirsutum).in order to provide farmers with better and more customized alternatives to improve yields, combining multiple genetically modified (gm) traits into a single product (called stacked trait crops) is becoming prevalent. trait protein expression levels are used to characterize new gm products and establish exposure limits, two important components of safety assessment. developing a multiplexed immunoassay capable of measuring all trait proteins in the same sample allows for higher sample throughput ...201627177195
a genetic map between gossypium hirsutum and the brazilian endemic g. mustelinum and its application to qtl mapping.among the seven tetraploid cotton species, little is known about transmission genetics and genome organization in gossypium mustelinum, the species most distant from the source of most cultivated cotton, g. hirsutum in this research, an f2 population was developed from an interspecific cross between g. hirsutum and g. mustelinum (hm). a genetic linkage map was constructed mainly using simple sequence repeat (ssrs) and restriction fragment length polymorphism (rflp) dna markers. the arrangements ...201627172208
the immature fiber mutant phenotype of cotton (gossypium hirsutum) is linked to a 22-bp frame-shift deletion in a mitochondria targeted pentatricopeptide repeat gene.cotton seed trichomes are the most important source of natural fibers globally. the major fiber thickness properties influence the price of the raw material, and the quality of the finished product. the recessive immature fiber (im) gene reduces the degree of fiber cell wall thickening by a process that was previously shown to involve mitochondrial function in allotetraploid gossypium hirsutum here, we present the fine genetic mapping of the im locus, gene expression analysis of annotated protei ...201627172184
genome wide ssr high density genetic map construction from an interspecific cross of gossypium hirsutum × gossypium tomentosum.a high density genetic map was constructed using f2 population derived from an interspecific cross of g. hirsutum × g. tomentosum. the map consisted of 3093 marker loci distributed across all the 26 chromosomes and covered 4365.3 cm of cotton genome with an average inter-marker distance of 1.48 cm. the maximum length of chromosome was 218.38 cm and the minimum was 122.09 cm with an average length of 167.90 cm. a sub-genome covers more genetic distance (2189.01 cm) with an average inter loci dist ...201627148280
epistasis together with partial dominance, over-dominance and qtl by environment interactions contribute to yield heterosis in upland cotton.qtl mapping based on backcross and ril populations suggests that epistasis together with partial dominance, over-dominance and their environmental interactions of qtls play an important role in yield heterosis in upland cotton. a backcross population (bc) was constructed to explore the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). for yield and yield components, recombinant inbred line (ril) and bc populations were evaluated simultaneously at three different locations. a t ...201627138784
fine mapping and rna-seq unravels candidate genes for a major qtl controlling multiple fiber quality traits at the t1 region in upland cotton.improving fiber quality is a major challenge in cotton breeding, since the molecular basis of fiber quality traits is poorly understood. fine mapping and candidate gene prediction of quantitative trait loci (qtl) controlling cotton fiber quality traits can help to elucidate the molecular basis of fiber quality. in our previous studies, one major qtl controlling multiple fiber quality traits was identified near the t1 locus on chromosome 6 in upland cotton.201627094760
fibre elongation requires normal redox homeostasis modulated by cytosolic ascorbate peroxidase in cotton (gossypium hirsutum).high-quality cotton fibre equates to a more comfortable textile. fibre length is an important index of fibre quality. hydrogen peroxide (h2o2) acts as a signalling molecule in the regulation of fibre elongation. results from in vitro ovule culture suggest that the alteration of fibre cell h2o2 levels affects fibre development. ascorbate peroxidase (apx) is an important reactive oxygen species (ros) scavenging enzyme, and we found that ghapx1at/dt encoded one member of the previously unrealized g ...201627091877
genomic heterozygosity and hybrid breakdown in cotton (gossypium): different traits, different effects.hybrid breakdown has been well documented in various species. relationships between genomic heterozygosity and traits-fitness have been extensively explored especially in the natural populations. but correlations between genomic heterozygosity and vegetative and reproductive traits in cotton interspecific populations have not been studied. in the current study, two reciprocal f2 populations were developed using gossypium hirsutum cv. emian 22 and g. barbadense acc. 3-79 as parents to study hybri ...201627072350
construction of a high-density genetic map by specific locus amplified fragment sequencing (slaf-seq) and its application to quantitative trait loci (qtl) analysis for boll weight in upland cotton (gossypium hirsutum.).upland cotton (gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for the industrial production and everyday use. next-generation sequencing is a powerful method to identify single nucleotide polymorphism markers on a large scale for the construction of a high-density genetic map for quantitative trait loci mapping.201627067834
comparative transmission genetics of introgressed chromatin in gossypium (cotton) polyploids.introgression is widely acknowledged as a potential source of valuable genetic variation, and growing effort is being invested in analysis of interspecific crosses conferring transgressive variation. experimental backcross populations provide an opportunity to study transmission genetics following interspecific hybridization, identifying opportunities and constraints to introgressive crop improvement. the evolutionary consequences of introgression have been addressed at the theoretical level, ho ...201627056931
generation of transcriptome profiling and gene functional analysis in gossypium hirsutum upon verticillium dahliae infection.verticillium wilt caused by the soil-borne fungus verticillium dahliae, is a devastating disease in cotton. to get more candidate genes related to wilt resistance, a normalized, full-length cdna library was used to generate the transcriptome profile from a wilt-resistant gossypium hirsutum variety (zhongzhimian kv3) upon v. dahliae strain v991 infection. total 3486 high-quality ests were focused from randomly selected 4000 clones, which included 3065 singletons and 421 contigs. to categorize the ...201627038549
insights into the ecology and evolution of polyploid plants through network analysis.polyploidy is a widespread phenomenon throughout eukaryotes, with important ecological and evolutionary consequences. although genes operate as components of complex pathways and networks, polyploid changes in genes and gene expression have typically been evaluated as either individual genes or as a part of broad-scale analyses. network analysis has been fruitful in associating genomic and other 'omic'-based changes with phenotype for many systems. in polyploid species, network analysis has the ...201627027619
mapping of genes for flower-related traits and qtls for flowering time in an interspecific population of gossypium hirsutum × g. darwinii. 201627019451
genome-wide identification and analysis of the stress-resistance function of the tps (trehalose-6-phosphate synthase) gene family in cotton.trehalose (a-d-glucopyranosyl a-d-glucopyranoside) is a nonreducing disaccharide and is widely distributed in bacteria, fungi, algae, plants and invertebrates. in the study, the identification of trehalose-6-phosphate synthase (tps) genes stress-related in cotton, and the genetic structure analysis and molecular evolution analysis of tpss were conducted with bioinformatics methods, which could lay a foundation for further research of tps functions in cotton.201626993467
simultaneous overexpression of the hherf2 and pedreb2a genes enhanced tolerances to salt and drought in transgenic cotton.a desert-grown halimodendron halodendron ethylene-responsive element binding factor gene (hherf2), which encodes a 245 amino acids protein containing a conserved ap2/erebp domain, was isolated through the rapid amplification cdna end (race) method. sequence and phylogenetic analysis indicated that hherf2 was classified into the b-2 group of erf subfamily. semiquantitative rt-pcr showed that hherf2 was greatly induced by treatments with high-salt, drought and low temperature in h. halodendron see ...201626972971
critical roles of vacuolar invertase in floral organ development and male and female fertilities are revealed through characterization of ghvin1-rnai cotton plants.seed number and quality are key traits determining plant fitness and crop yield and rely on combined competence in male and female fertilities. sucrose metabolism is central to reproductive success. it remains elusive, though, how individual sucrose metabolic enzymes may regulate the complex reproductive processes. here, by silencing vacuolar invertase (vin) genes in cotton (gossypium hirsutum) reproductive organs, we revealed diverse roles that vin plays in multiple reproductive processes. a se ...201626969720
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