Publications

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studies on the disaggregation of ef-1 with carboxypeptidase a. 1977406850
isolation and characterization of structural components of bacillus cereus ahu 1356 cell walls. 1977407078
the action of lysozyme on peptidoglycan with n-unsubstituted glucosamine residues. isolation of glycan fragments and their susceptibility to lysozyme.1. a peptidoglycan preparation n-acetylated at about 30% of glucosamine residues was obtained by the treatment of the lysozyme-resistant cell wall paptidoglycan of bacillus cereus with acetic anhydride at ph 7. fractionation of dialyzable material resulting from lysozyme digestion of the glycan component of this peptidoglycan preparation yielded five oligosaccharides designated as s1 to s5 besides the disaccharide glcnac-murac. 2. oligosaccharide s3, which accounted for about 30% of the disaccha ...1977407083
spores of microorganisms. xxvii. some physiological and morphological characteristics of thick-walled cellular forms of bacillus cereus. 1977407136
microbiol n-acetylation of daunorubicin and daunorubicinol. 1977407204
production of copper coproporphyrin iii by bacillus cereus. i. purification and identification of copper coproporphyrin iii.bacillus cereus strains 2 and t did not form spores and accumulated a large amount of purple pigment inside the cells, when cultured in a yeast extract-ammonium salt medium with excess glucose. the pigment was extracted and crystallized as the ethyl ester. it was identified as copper coproporphyrin iii.1977407221
production of copper coproporphyrin iii by bacillus cereus. ii. regulation of the biosynthesis of coproporphyrin iii and its copper complex by oxygen and heavy metal ions.the effects of oxygen and heavy metal ions on the production of copper coproporphyrin iii were studied in bacillus cereus strain 2. the formation of copper coproporphyrin iii was found to be maximum when the cells were cultivated in g-medium at a low level of oxygen supply, but it was suppressed at extremely low oxygen supply levels. when the cells were cultured in metal-free g-medium, neither metal-coproporphyrin iii nor coproporphyrin iii was formed. in the presence of copper in the medium (40 ...1977407222
a case of bacillus cereus bacteraemia.a case is presented of bacillus cereus bacteraemia in a patient receiving hepatic perfusion with 5-fluorouracil (5fu) for metastasis from a carcinoma of the breast. the literature concerning systemic b. cereus infections is briefly reviewed.1977407561
[detection of bacillus cereus-toxins (author's transl)].during the logarithmic state of growth, b. cereus produced extracellular toxins, which could be precipitated by ammoniumsulphate. the toxins were not dialysable. 9 strains of b. cereus were tested and showed lethal, hemolytic, and phospholipase-c-reactions. the lethal toxin injected i.v., killed mice within 20 minutes. an intradermal injection of the 9 toxin-precipitates in rabbits produced skin reactions, which could be neutralized by a specific antiserum against the b. cereus strain b-4ac. rel ...1977407743
inhibition of the synthesis of phospholipase c in bacillus cereus by a component of the growth medium.a low molecular weight compound, probably of peptide nature, present in the beef heart extract component of the growth medium, inhibits post-transcriptionally the biosynthesis of phospholipase c by a strain of bacillus cereus. the compound also prevents the increase of proteolytic activity in the growth medium, thus suggesting that the synthesis of another enzyme, an extracellular protease, is also inhibited, and that the inhibitory compound may therefore have a more general effect on exoenzyme ...1977407774
epoxidation of aldrin to exo-dieldrin by soil bacteria.twenty-two strains of soil bacteria, including representatives of the genera bacillus, micromonospora, mycobacterium, nocardia, streptomyces, thermoactinomyces, and pseudomonas and 10 unidentified gram-negative, motile, rod-shaped bacteria, were shown to degrade aldrin to its epoxide dieldrin. in every case, the exo-stereoisomer of dieldrin was produced exclusively.1977407844
studies on bacterial cell wall inhibitors. ii. inhibition of peptidoglycan synthesis in vivo and in vitro by amphomycin. 1977407940
[regulation of isoleucine and valine biosynthesis in bacillus cereus t: possible role of threonine deaminase].threonine desaminase: a possible regulatory element suggested by the analysis of isoleucine, valine pathway regulation in bacillus cereus t.1977408042
re-activation of guinea-pig liver uridine diphosphate glucuronosyltransferase with 1-monoacyl phosphatidylcholine after extensive phospholipid deletion at 5 degrees c with pure bacillus cereus phospholipase c. 1977408208
[heterogeneity of the microcyclic development of bacillus cereus 504]. 1977408588
studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella.cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ...1977408940
antimicrobial activity of amine oxides: mode of action and structure-activity correlation.the effect of n-alkyl derivatives of saturated heterocyclic amine oxides on the growth and metabolism of microorganisms has been studied. 4-dodecylmorpholine-n-oxide inhibited the differentiation and growth of bacillus cereus, of different species of filamentous fungi, and of the yeast saccharomyces cerevisiae. for vegetative cells, the effect of 4-dodecylmorpholine-n-oxide was lethal. cells of s. cerevisiae, after interaction with 4-dodecylmorpholine-n-oxide, released intracellular k(+) and wer ...1977409340
extent of cross-linking of germ cell wall of a variant of bacillus cereus. 1977409409
immunocytochemical localization of spore specific antigens in ultrathin sections. 1977409707
[a fatal food poisoning caused by bacillus cereus (author's transl)].the autopsy of a 37 year old man who had died under extraordinary circumstances showed a bromatoxism by bacillus cereus. the verification of the bacillus was possible although the man had already died 2-3 days before; and that in spite of the existence of early beginning rottenness. with the help of morphological findings the pathogenetic effect of the toxins (exotoxin and enterotoxin) of bacillus cereus is discussed. blood cultures of the heart alone are not sufficient to prove a causal connexi ...1977410193
comparative studies of bacillus cereus strains isolated from various foods and food poisoning outbreaks. 1977410239
effect of some bacterial products on platelet electrophoretic mobility (pem).bacterial lps, used in our experiments, are able to decrease pem at concentration of 100 microgram/ml after incubation with 1 x 10(7) platelets/ml for 30 min at 37 degrees c. such effect is statistically significant for bacteroides fragilis lps, s. enteritidis and s. typhi. e. coli lps 026:b6 w and e. coli 055:b5 w are not effective at all, s. minnesota (incomplete) lps 595 and 345 free of 0-polysaccharide and partially lacking core in their molecule did not influence pem, while s. minnesota lps ...1977410424
characterization of the "microprotease" from bacillus cereus. a zinc neutral endoprotease.the neutral protease isolated from bacillus cereus (brl-70) has been purified by affinity chromatography and characterized. the enzyme exhibits a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has a molecular weight of 34 000 by ultracentrifugation, and contains one enzymatically essential zinc atom per 34 000 g. these data together with the amino acid composition, response to metal substitution, chemical modification, and substrate specificity all indicate that this p ...1977410441
levels of acetyl coenzyme a, reduced and oxidized coenzyme a, and coenzyme a in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of bacillus megaterium.dormant spores of bacillus megaterium were found to contain approximately 850 pmol of coenzyme a (coa) per milligram of dry weight. of this total, less than 1.5% was acetyl-coa, 25% was coa-disulfide, 43% was in disulfide linkage to protein, and the remainder was the free thiol. dormand spores of bacillus cereus and clostridium bifermentans contained 700 and 600 pmol of coa per milligram of dry weight, respectively; in both species approximately 45% of the coa 45% of the coa was in disulfide lin ...1977410791
[the occurrence of bacillus cereus in dairy products (authors transl)].during one year 1390 samples of pasteurized milk products from five different dairies were examined for content of b. cereus. the examination was done on the day of packaging and after incubation at 17 degrees c for 24 hrs. as well. on examination on the day of packaging 8.1 % were positive. no difference could be demonstrated between low-temperature pasteurized and high-temperature pasteurized products. however, differences were found from one plant to another (2.1 %--14.5 %). after incubation ...1977411110
the fate of enterotoxigenic bacteria inoculated onto chinese barbecued foods. 1977411566
[detection of cephalosporins in raw milk with the aid of b. cereus beta lactamase]. 1977411648
relationship between the structure of carbonylcyanide phenylhydrazones and inhibition of growth of microorganisms, stimulation of respiration of yeast cells and rat liver mitochondria.the effect of ten derivatives of carbonylcyanide phenylhydrazone on growth of bacteria, yeast and different species of filamentous fungi was investigated. in yeast and mitochondria isolated from rat liver the effect of these derivatives on the respiratory activity was also followed. the relative efficiency of the individual derivatives of carbonylcyanide phenylhydrazone was determined on the basis of the results obtained. it was shown that derivatives, in which the substituent on the benzene rin ...1977411721
a survey for the presence of teichuronic acid in walls of bacillus megaterium and bacillus cereus. 1977411891
[phage diagnosis of strains of bacillus cereus isolated from food products].a total of 80 cultures of bacillus cereus, isolated from food products, were studies to establish the behaviour to specific phages or the cam set and the presence of lysogeny. a modern bacteriophage method was tested for the first time in the differentiatons of bacillus cereus, which was so far studied mainly in the differentiation of bacillus anthracis. results showed that bacteriophagy with specific type phages as a method, though not absolutely precise, is dependable for the differentiation o ...1977412296
characterization and function of intracellular proteases in sporulating bacillus cereus.intracellular proteases from sporulating bacillus cereus have been purified by ammonium sulfate fractionation, heat treatment and deae cellulose column chromatography. after the last purification step, two protease activities, with an activity ratio of about thirty to one are resolved. both proteases are resistant to o-phenanthroline but sensitive to phenyl methyl sulfonyl fluoride. their separation by polyacrylamide gel electrophoresis and deae cellulose column chromatography, their difference ...1977412478
[the effect of sodium fluoride on the antimicrobial action of tetracycline]. 1977412595
[hygienic evaluation of insecticide preparations]. 1977412732
the effect of water activity and aw-controlling solute on sporulation of bacillus cereus t. 1977412827
the lipid requirement of the (ca2+ + mg2+)-atpase in the human erythrocyte membrane, as studied by various highly purified phospholipases.1. when complete hydrolysis of glycerophosphlipids and sphingomyelin in the outer membrane leaflet is brought about by treatment of intact red blood cells with phospholipase a2 and sphingomyelinase c, the (ca2+ + mg2+)-atpase activity is not affected. 2. complete hydrolysis of sphingomyelin, by treatment of leaky ghosts with spingomyelinase c, does not lead to an inactivation of the (ca2+ + mg2+)-atpase. 3. treatment of ghosts with phospholipase a2 (from either procine pancreas of naja naja veno ...1977137746
membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts.nascent calcium phosphate promotes the agglutination and fusion of human erythrocyte ghosts. membrane phospholipids of erythrocyte ghosts treated with ca2+ and phosphate ions become exposed to attack by phospholipase c (phosphatidylcholine cholinephosphohydrolase, ec 3.1.4.3) (bacillus cereus). freeze-fracture pictures of fused erythrocyte ghosts show the presence of regions deficient in intramemebrane particles in the protoplasmic face which we believe to be regions of fusion. discontinuous reg ...1977329283
[germination and outgrowth of spores and proliferation of outgrown vegetative cells in the peritoneal cavity of mice intraperitoneally injected with bacillus cereus or bacillus subtilis spores (author's transl)]. 1977415160
the role of various milk fractions and the importance of somatic cells in the formation of germinant(s) for bacillus cereus when milk is pasteurized. 1977412883
[a case of acute myelogenous leukemia, complicated with bacillus cereus septicemia (author's transl)]. 1977416149
the growth and respiration of bacterial colonies.young colonies of two swarming organisms, bacillus subtilis and proteus vulgaris, grew about as quickly on solid media as in liquid culture whilst four non-swarming organisms, bacillus cereus, enterobacter cloacae, escherichia coli and staphylococcus albus, all grew slower on solid than in liquid media. oxygen uptake by young colonies of b. subtilis, followed manometrically, increased exponentially at about the same rate as unrestricted aerobic growth. all other colonies demonstrated acceleratin ...1977591923
membrane properties of aplysia neurones intracellularly injected with phospholipases a and c.1. the effects of phospholipases a from bee venom and from porcine pancreas and of phospholipases c from clostridium welchii and bacillus cereus on active and passive membrane properties of aplysia neurones have been studied. consistent alterations in electrical membrane properties were found following intracellular application of three of these enzymes.2. bee venom phospholipase a produced a rapid decrease of membrane potential and resistance. voltage clamping revealed a marked depression of pe ...1977874892
substrate-induced deactivation of penicillinases. studies of beta-lactamase i by hydrogen exchange.the conformational motility of beta-lactamase i from bacillus cereus was studied by hydrogen exhange. the time course of the isotopic replacement of peptide hydrogen atoms was followed by 'exchange-in' or 'exchange-out' experiments. many of the substrates for this enzyme that have o-substituted aromatic or heterocyclic side chains (e.g. methicillin or cloxacillin) are known to effect a decrease in enzymic activity ('substrate-induced deactivation'). there was a marked discontinuity in the exchan ...1977921750
asymmetry of the phospholipid bilayer of rat liver endoplasmic reticulum.the phospholipids of intact microsomal membranes were hydrolysed 50% by phospholipase c of clostridium welchii, without loss of the secretory protein contents of the vesicle, which are therefore not permeable to the phospholipase. phospholipids extracted from microsomes and dispersed by sonication were hydrolysed rapidly by phospholipase c-cl. welchii with the exception of phosphatidylinositol. assuming that only the phospholipids of the outside of the bilayer of the microsomal membrane are hydr ...1977921959
biochemical aspects of the visual process. xxxvii. evidence for lateral aggregation of rhodopsin molecules in phospholipase c-treated bovine photoreceptor membranes.photoreceptor membranes derived from isolated bovine rod outer segments, are subjected to treatment with phospholipase c (bacillus cereus). this results in varying degrees of hydrolysis of the membrane phospholipids into diglycerides and water soluble phosphate esters without loss of rhodopsin. electron microscopic observations of thin sections and freeze-fractured preparations indicate extrusion of diglycerides from the membranes and their coalescence to lipid droplets, beginning at 20% hydroly ...1978647003
production and property of beta-lactamases in streptomyces: comparison of the strains isolated newly and thirty years ago.productivity and property of beta-lactamases of streptomyces strains isolated from soil some 30 years ago were studied in comparison with those of the newly isolated strains. at least three-quarters of the streptomyces strains produced beta-lactamase constitutively and extracellularly, mainly as penicillinases, as in the cases of those from the newly isolated strains. strains such as s. albus, s. diastatochromogenes, s. fradiae, and s. lavendulae were the highest producing strains, and the amoun ...1978666306
transduction in bacillus thuringiensis.bacteriophage cp-51, originally reported as a generalized transducing phage for bacillus cereus and b. anthracis, has been shown to carry out generalized transduction in several strains of b. thuringiensis. a newly isolated phage, cp-54, which has a broader host range than cp-51, also mediates generalized transduction in b. thuringiensis. cp-51 and cp-54 are similar in size and morphology and are related serologically, but they are not identical. cp-54 is more cold labile than cp-51, and, as wit ...1978677877
an enzymic determination for serum phospholipid.a new colorimetric determination for serum phospholipid is described. firstly, serum phospholipid is incubated with phospholipase c from bacillus cereus, and then the released diglyceride and triglyceride are hydrolyzed completely to fatty acid and glycerol by lipoprotein lipase from pseudomonas fluorescens. secondly, the glycerol produced is enzymatically determined by glycerol dehydrogenase in the presence of nad+, using phenazine methosulfate-nitro blue tetrazolium as color reagents. the abso ...1978709886
purification of antibiotics from physarum gyrosum by high pressure liquid chromatography.a family of five antibiotic substances was isolated from the slime mold physarum gyrosum by high pressure liquid chromatography (hplc). for this purpose, mold was cultured for two weeks in a liquid medium. soluble products were harvested by rotary evaporation of medium and extraction with 1-butanol. paper chromatography in ethyl acetate:pyridine:water (2:2:1 v/v) was used for preliminary fractionation. active components were separated by hplc with a reverse-phase column packed with bondapack c18 ...1978714881
toxicity of zinc to fungi, bacteria, and coliphages: influence of chloride ions.a 10 mm concentration of zn2+ decreased the survival of escherichia coli; enhanced the survival of bacillus cereus; did not significantly affect the survival of pseudomonas aeruginosa, norcardia corallina, and t1, t7, p1, and phi80 coliphages; completely inhibited mycelial growth of rhizoctonia solani; and reduced mycelial growth of fusarium solani, cunninghamella echinulata, aspergillus niger, and trichoderma viride. the toxicity of zinc to the fungi, bacteria, and coliphages was unaffected, le ...1978736544
[effect of a bacillus cereus 504 culture on the development of homologous strain cells]. 1978416328
bacillus cereus food poisoning. 1978413036
reversible inhibitors of penicillinases.reversible competitive inhibitors of a penicillinase, beta-lactamase 1 from bacillus cereus, were studied. these represent the first inhibitors of a penicillinase that lack the beta-lactam ring. the products of the enzymic reaction, namely penicilloic acids, are inhibitors; their decarboxylation products, the penilloic acids, are also inhibitors, and have somewhat lower ki values. inhibitors have been prepared from benzylpenicillin, phenoxymethyl-penicillin, methicillin (2,6-dimethoxybenzamidope ...1978415738
induction of penicillinase by bacitracin. 1978415741
prevalence of bacillus cereus in the faeces of healthy adults.in a survey designed to determine the prevalence of bacillus cereus in the faeces of healthy persons, the organism was found in low numbers in 100 (14%) of single faecal specimens from 711 adults in the general population. in addition, in an attempt at assessing the changes in the b. cereus distribution within the faecal flora of the individual, weekly faecal specimens were submitted over a seven-week period by 18 members of staff of two laboratories. the total isolation rate was again 14%, with ...1978416141
phosphatidylinositol as the endogenous activator of the (na+ + k+)-atpase in microsomes of rabbit kidney.incubation of rabbit kidney microsomes with pig pancreatic phospholipase a2 produced residual membrane preparations with very low (na+ + k+)-atpase activity. the activity could be restored by recombination with lipid vesicles of negatively-charged glycerophospholipids. vesicles of pure phosphatidylcholine and phosphatidylethanolamine were virtually inactive in this respect, but could reactivate in the presence of cholate. incubation of the microsomes with a combination of phospholipase c (bacill ...1978147707
[case with the clinical manifestation of anthrax etiologically related to b. cereus]. 1978152230
characterization of a bacillus cereus protease mutant defective in an early stage of spore germination.temperature-sensitive sporulation mutants of bacillus cereus were screened for intracellular protease activity that was more heat labile than that of the parental strain. one mutant grew as well as the wild type at 30 and 37 degrees c but sporulated poorly at 37 degrees c in an enriched or minimal medium. these spores germinated very slowly in response to alanine plus adenosine or calcium dipicolinate. during germination, spores produced by the mutant rapidly became heat sensitive, but released ...1978412836
determination of fatty acid compositions of bacillus cereus and related bacteria: a rapid gas chromatographic method using a glass capillary column.a rapid gas chromatographic method for the determination of fatty acid compositions of bacillus cereus and related bacteria is presented. by the use of a free fatty acid phase-coated glass capillary column, the complete separation of fatty acids, including the branched ones, was achieved. the method enables a more distinct differentiation of bacillus species than can be obtained with packed columns.1978416751
fatty acids of gram-positive bacterial rods from human dental plaque. 1978416814
[radiosensitivity of the bacteriophages of aerobic sporulating microorganisms]. 1978417102
low temperature microspectrofluorometry: design of a 'cold chamber'.low temperature microspectrofluorometry allows an improvement of spectral resolution and an increase of fluorescence intensity. suppression of fluorescence fading, or at least a marked reduction, is also obtained. a cooling chamber for microspectrofluorometric measurement is described which allows the cooling, under a microscope, of a biological sample down to liquid nitrogen temperature. objectives with numerical apertures better than 1.0 and a magnification power up to 100x can be used. low te ...1978417183
osmotically induced increase in thermal resistance of heat-sensitive, dipicolinic acid-less spores of bacillus cereus ht-8.thermal resistance in heat-sensitive, dipicolinic acid (dpa)-less spores of bacillus cereus ht-8 heated in sucrose solutions increased at and above a concentration of 2 m sucrose. the decimal reduction times at 75 degrees c for spores heated in 0.0, 1.8, 2.2, and 2.6 m sucrose were 2.0, 2.8, 4.5, and 12 min, respectively. maltose, fructose, and glucose increased heat resistance above that observed in water but did not elevate resistance to the level observed with sucrose at the same osmolality. ...1978417675
enzymatic formation of uridine diphosphate n-acetyl-d-mannosamine.an enzyme that catalyzes the interconversion of udp-n-acetyl-d-glucosamine and udp-n-acetyl-d-mannosamine was purified about 700-fold from the supernatant fraction of bacillus cereus, and the properties of this enzyme were studied. this enzyme was not stimulated by nad+, nadh, or any metal ions. the optimum ph was between 7.5 and 8.0. at equilibrium of the reaction, the ratio of udp-n-acetylglucosamine to udp-n-acetylmannosmaine was about 9:1. the enzyme was inactive toward free n-acetylhexosami ...1978418068
carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite reticulitermes hesperus.bacillus cereus rw1 and serratia marcescens rw3, isolated from the hind-gut of the termite reticulitermes hesperus, both grew well on mesquite wood and produced moderate amounts of carboxymethylcellulase. carboxymethylcellulose (cmc) gels were depolymerized rapidly by b. cereus rw1 and slowly by s. marcescens rw3. the depolymerization of cmc was ph and temperature sensitive. depolymerization of gels by growing cultures of b. cereus rw1 and the action of cell-free extracts of b. cereus rw1 on cmc ...1978418144
preliminary crystallographic data for beta-lactamase i from bacillus cereus 569. 1978418181
[comparative investigations on the efficiency of chlorine and ozone against bacteria and spores (author's transl)].this paper is concerned with the measurement of first order rate constants for the death of bacteria by chlorine and ozone. while the first order conditions for chlorine were achieved with the aid of a new instrument which works basing on the polarographic principle this was attained for ozone under steady state conditions. besides differences of species some different properties for both oxidants were found as far as their bactericidal efficiency is concerned. they are discussed in this paper.1978418606
alteration by cereolysin of the structure of cholesterol-containing membranes.when erythrocyte membranes were treated with cereolysin, negatively stained and examined by electron microscopy, ring and arc-shaped structures were observed in the membrane. the outside diameter of the rings varied from 33 to 50 nm with a border thickness of 6.7 to 8.3 nm. the arcs varied in length from 33 to 170 nm with a border thickness of also 6.7 to 8.3 min. when right-side-out erythrocyte ghosts which had been treated with cereolysin were examined by electron microscopy after freeze-fract ...1978414780
spore location patterns in sporulating doublets of bacillus cereus and bacillus megaterium, derived from single doublet isolates with differing sporulation geometry.the distribution of spore loci in pairs of bacillus cereus and b. megaterium showed different degrees of polarization from random location towards location at the old end of the sporangium. when individual doublets containing both spores at either the old or new ends of the sporangia were isolated by micromanipulation, subsequent culture showed the same spore location patterns.1978415036
membrane-bound nucleotidase of bacillus cereus.a membrane-bound nucleotidase of bacillus cereus t was solubilized by digestion with trypsin and subsequently purified more than 300-fold. the purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. in addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to ...1978415044
bacteriocin and antibiotic resistance plasmids in bacillus cereus and bacillus subtilis.a number of plasmids have been isolated as covalently closed circular dnas from strains of bacillus cereus and b. subtilis. from 12 out of 15 strains of b. cereus, plasmids could be isolated. most of the b. cereus strains contained two or more plasmids. their molecular weights ranged from 1.6 x 10(6) to 105 x 10(6). bacteriocin production could be attributed to a 45 x 10(6)-dalton plasmid (pbc7) from b. cereus dsm 336, and tetracycline resistance to a 2.8 x 10(6) plasmid (pbc16) from b. cereus g ...1978415051
cardiolipin: a stereospecifically spin-labeled analogue and its specific enzymic hydrolysis.the spin-labeled cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phosphol]-sn-glycerol has been prepared. the stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glycero(3)phospho]-sn-glycerol, available from the stereospecific hydrolysis of cardiolipin by phospholipase a2 (phosphatide 2-acylhydrolase, ec 3.1.1.4) of trimeresurus flavoviridis. the results of treatment of the spin-labeled cardiolipin with the cardiolip ...1978274715
phospholipid dependence of udp-glucuronyltransferase.very extensive hydrolysis of phospholipids with pure bacillus cereus phospholipase c at 5 degrees c greatly inhibited the maximum demonstrable rate of glucuronidation of p-nitrophenol by udpglucuronyltransferase in guinea pig liver microsomes. lysophosphatidylcholine restored much of the inhibited activity but non-phospholipid surfactants or hydrolysis of diglycerides failed to reactivate. phospholipid depletion likewise inhibited o-aminophenol glucuronidation and phospholipids restored activity ...1978203315
role of exogenous phospholipases in triggering platelet aggregation. 1978207175
enzymatic properties of phosphatidylinositol inositolphosphohydrolase from bacillus cereus. substrate dilution in detergent-phospholipid micelles and bilayer vesicles. 1978207692
synthesis and quantitative structure-activity relationships of antibacterial 1-(substituted benzhydryl)-4-(5-nitro-2-furfurylideneamino) piperazines.1-benzhydryl -4- (5-nitro-2-furfurylideneamino) piperazine and 11 substituted analogs were prepared and examined for in vitro antimicrobial activity. the compounds were active against bacillus cereus 7, bacillus megaterium 122, bacillus subtilis 104, clostridium perfringens 13, and the tetracycline-resistant clostridium perfringens 37. regression analyses on the antibacterial activity data based on the hansch approach, using pi, pi2, and sigma parameters, yielded several statistically significan ...1978207855
[enzymatic mechanisms for antimicrobial protection of the oral cavity].data on the role of oral lysozyme, ribonuclease, deoxyribonuclease and peroxidase in antimicrobial defense of the macroorganism are reviewed. the biochemical and physiological properties of the enzymes secreted by salivary glands and released from emigrating leukocytes are discussed. spectra of antimicrobial action of the enzymes and participation of these enzymes in maintaining the stability of oral biocenosis are described as well as the regulation of these enzymatic activities and the pathoge ...1978208288
hydrolytic action of phospholipases on sealed ghosts of mammalian erythrocytes. 1978211122
foodborne hazards of microbial origin.foods can serve as vehicles of many pathogenic and toxigenic agents of disease. bacterial agents comprise three groups: 1) those that grow in the food and produce an active toxin before consumption (e.g., clostridium botulinium); 2) those that merely exist as contaminants in the food but are able to initiate infection when swallowed (e.g., salmonella spp.); and 3) those that multiply and produce large numbers of vegetative cells in the food, then release an active enterotoxin when they sporulate ...1978212326
the use of phospholipase c to detect structural changes in the membranes of human erythrocytes aged by storage. 1978213113
phosphatase activity of aerobic and facultative anaerobic bacteria.1115 strains of aerobic and facultatively anaerobic bacteria were tested for phosphatase activity by a conventional plate method and a microtest. the microtest was devised to allow results to be read after 4 h cultivation. phosphatase activity was found in wide range of species and strains. besides staphylococci, where the test for phosphatase is successfully used, it may be applied as one of the valuable tests for the differentiation of the following species: bacillus cereus, b. licheniformis, ...1978216188
effect of hydrolytic enzymes and protein-modifying reagents on gonadotropin receptors in bovine corpus luteum cell membranes.preincubation of membranes with various concentrations of pronase, trypsin, lipase, phospholipase a from vipera russelli and from crotalus durissus terrificus, phospholipase c from bacillus cereus and from clostridium welchii, acetic anhydride, 2,4-dinitrofluorobenzene and tetranitromethane resulted in a dose-dependent inhibition of 125i-labeled human choriogonadotropin binding. at the submaximal concentrations of enzymes and at both submaximal and maximal concentrations of protein-modifying rea ...1978202334
[microbiological analysis of fruit-flavored toothpastes for children]. 197899797
bacillus cereus as a pathogen. 197899823
the inhibition of clostridium chauvoei by bacillus cereus metabolites. 1978105701
conformational studies on phospholipase c from bacillus cereus. the effect of urea on the enzyme.1. when heated in 8 m-urea, phospholipase c(ec 3.1.4.3) from bacillus cereus undergoes conformational transitions depending on the temperatures used. these transitions were studied by examining protein fluorescence, iodide quenching of protein fluorescence, u.v. difference spectroscopy, chemical availability of histidine residues in the enzyme, circular dichroism and catalytic activity. 2. unless simultaneously exposed to elevated temperatures the enzyme appears to be unaffected by 8 m-urea. rem ...1978105729
radiosensitivity of bacteriophages of aerobic spore-forming microorganisms.under conditions of the direct action of radiation on seven original phages of aerobic spore-forming bacteria and four standard phages of e. coli, it was established that they possessed different sensitivity to gamma radiation and were distributed into four groups. the phages of e. coli of the t series, especially t2 phage, proved the most sensitive. the phages of aerobic spore-forming bacilli were characterized by greater resistance to the action of gamma radiation, which is evidently associate ...1978105763
production and characterization of two hemolysins of bacillus cereus.bacillus cereus strain b-48 produced two hemolysins with molecular weights of 52,000 (h-i) and 31,000 (h-ii). a mutant was isolated that produced only h-ii but was identical with the wild type in all other respects. we exploited this mutant to produce h-ii for study that was free of contamination by h-i. by manipulation of media composition, we produced h-i in the absence of h-ii. the hemolysins were precipitated differently by ammonium sulfate, and both exhibited the arrhenius effect when heate ...1978105792
thermal analysis of the spores of bacillus cereus with special reference to heat activation.the heat activation of bacterial spores was studied by means of differential thermal analysis in the temperature range 30-110 degrees c using the spores of bacillus cereus. the thermogram showed three endothermic peaks at 56, 95, and 103 degrees c with one exothermic peak at 105 degrees c during the heating process. the spore coat separated from the native spores also showed a peak at 56 degrees c on its heating thermogram. the peak at 56 degrees c was reversible for both native spores and the s ...1978105794
[bacillus cereus septicemia (author's transl)]. 1978106146
preliminary experimental data on inhibition of beta-lactamase i from b. cereus by cu (++) and zn(++). 1978106864
[method of determining the antimicrobial activity of alcohol extracts of propolis].it is proposed to determine the antimicrobial activity of propolys alcohol extracts by the method of subsequent dilutions in solid nutrient media. dilution of the extracts immediately in hot agar eliminated the inhibiting effect of the extragent on the microbial growth. opalesence appearing in the agar did not prevent estimation of the results in contrast to the method of subsequent dilutions in liquid nutrient media.1978100047
delay in growth induced by glass filtered solar radiation in bacillus cereus. 1978100420
utilization of exogenous purine compounds in bacillus cereus. translocation of the ribose moiety of inosine.intact cells of bacillus cereus catalyze the breakdown of exogenous amp to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. inosine hydrolase was not detectable, even in crude extracts. inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. even though the equilibrium of the phosphorolytic reaction favors nucleoside synthe ...1978100497
effect of hydrostatic tensile stress on the growth of escherichia coli and bacillus cereus.the specific growth rates of escherichia coli and bacillus cereus were measured for growth media in a flask, a lens-plate arrangement simulating an isolated capillary space, and a lens-plate arrangement under hydrostatic tensile stress. the specific growth rates of the bacteria were the same for the flask and lens-plate arrangement without hydrostatic tensile stress, but were enhanced when the growth media were subjected to hydrostatic tensile stress. the enhanced specific growth rates reached s ...1978101141
studies on phosphatidylinositol phosphodiesterase (phospholipase c type) of bacillus cereus. ii. in vivo and immunochemical studies of phosphatase-releasing activity. 1978101146
isolation of stable ribosomal subunits from spores of bacillus cereus.analyses of ribosomes extracted from spores of bacillus cereus t by a dryspore disruption technique indicated that previously reported defects in ribosomes from spores may arise during the ribosome extraction process. the population of ribosomes from spores is shown to cotain a variable quantity of free 50s subunits which are unstable, giving rise to slowly sedimenting particles in low-mg2+ sucrose gradients and showing extremely low activity in in vitro protein synthesis. the majority of the ri ...1978101512
anti-riboflavin activity of 8n-alkyl analogues of roseoflavin in some gram-positive bacteria.three new 8n-alkyl analogues of roseoflavin (mm), i.e., 8-ethylamino- (eh), 8-methylethylamino- (me), 8-diethylamino-8-demethyl-d-riboflavin (ee), their tetraacetates, and 8-amino-8-demethyl-d-riboflavin (hh) tetraacetate, were synthesized. a relation between the anti-riboflavin activity and the chemical structure of 8n-alkyl analogues (8n-methyl, ethyl) was studied by a restoration by riboflavin (rf) of inhibitory effect of the analogues on a growth of gram-positive bacteria, i.e., sarcina lute ...1978101644
endogenous endophthalmitis associated with bacillus cereus bacteremia in a cocaine addict.a 22-year-old black female intravenous cocaine addict presented with an endophthalmitis of the right eye. diagnostic evaluation included an immediate anterior chamber paracentesis and a delayed vitreous aspiration. although cultures from the involved eye were negative, all 7 blood cultures grew bacillus cereus suggesting that this organism was the responsible agent of an endogenous endophthalmitis. the patient was treated with appropriate systemic and local antibiotics with resolution of the acu ...1978102235
role of cholesterol in the action of cereolysin on membranes. 1978102256
semi-automated detection of emesis in the rhesus monkey.the use of a video recorder and the inclusion of a marker dye in material fed to rhesus monkeys has eliminated the need for the constant presence of an observer to detect an emetic response.1978102332
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