Publications

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[toward a new pedagogy]. 20144158564
survival experience of women with bronchial carcinoma. 20144113304
concern for appropriateness as a moderator variable in the statistical explanation of self-reported use of alcohol and marijuana.two studies examine the concern for appropriateness scale (lennox & wolfe, 1984) as a likely moderator of dispositional and environmental variables associated with self-reported use of alcohol and marijuana by college and precollege students. in study 1, data from 408 upper division students show that concern score interacts significantly with religiosity in analyses of use of both drugs, but not with any of 11 other dispositional variables nor with drug-specific environment predictors in analys ...20143998978
arteriography of the carotid bifurcation: oblique projections.atherosclerotic plaques may not be demonstrated adequately by standard anteroposterior (ap)-lateral projections of carotid arteriography. we evaluated selective carotid arteriography in oblique projections for 60 individual carotid arteries. two neuroradiologists evaluated arteriograms in four separate sessions devoted to ap-lateral views alone and in combination with oblique projections. the lumen surface was characterized as "smooth," "irregular," or "ulcerated." when oblique projections were ...20144022356
anomalous volume change of gramicidin a in ethanol solutions. 20144143641
selective differentiation between amyloid and connective tissue structures based on the collagen specific topo-optical staining reaction with congo red. 20144108771
humanistic care or impaired physician? 20143946687
motherhood and apple pie: the effectiveness of medical care for children.the impact upon health of changes in public policy regarding health services has not been systematically assessed. the long-term, and largely irreversible, effects of reduced support for health services to children present special challenges to collaborative research between clinical and public health approaches. preliminary evidence from such a collaborative study of a variety of illnesses and interventions is persuasive of real benefit from prompt access to medical care.20143934582
[theories of biological similarities: 30 years of trial and error]. 20143915857
induced hypertension chemotherapy for solid tumors based on functional characteristics of tumor vessels. 20143999467
occurrence of haemoparasites in cattle in monduli district, northern tanzania.haemoparasite infections are among the most economically important cattle diseases in sub-saharan africa. the present study investigated the occurrence of haemoparasites in 295 indigenous cattle from five villages (mswakini, lake manyara, naitolia, makuyuni and nanja) of the monduli district, a wildlife-domestic animal-human interface area in northern tanzania. the data showed that the overall occurrence of haemoparasites in the sampled cattle was 12.5% (95% ci: 8.7% - 16.3%), involving single a ...201425685864
problem of ticks and tick-borne diseases in india with special emphasis on progress in tick control research: a review.ticks, as vectors of several zoonotic diseases, are ranked second only to mosquitoes as vectors. the diseases spread by ticks are a major constraint to animal productivity while causing morbidity and mortality in both animals and humans. a number of tick species have been recognised since long as vectors of lethal pathogens, viz. crimean-congo haemorrhagic fever virus (cchfv), kyasanur forest disease virus (kfdv), babesia spp, theileria, rickettsia conorii, anaplasma marginale, etc. and the dama ...201425540956
molecular survey and sequence analysis of anaplasma spp. in cattle and ticks in a malaysian farm.this study was conducted to determine the occurrence of anaplasma spp. in the blood samples of cattle, goats, deer and ticks in a malaysian farm. using polymerase chain reaction (pcr) and sequencing approach, anaplasma spp. was detected from 81(84.4%) of 96 cattle blood samples. all blood samples from 23 goats and 22 deer tested were negative. based on the analysis of the anaplasma partial 16s ribosomal rna gene, four sequence types (genotypes 1 to 4) were identified in this study. genotypes 1-3 ...201425776603
evaluation of the immune response to anaplasma marginale msp5 protein using a hsv-1 amplicon vector system or recombinant protein.anaplasma marginale is an intraerythrocytic vector-borne infectious agent of cattle. immunization with the current vaccine, based on parasitized erythrocytes with live anaplasma centrale, shows some constraints and confers partial protection, suggesting the feasibility for the development of new generation of vaccines. the aim of the present study was to assess the effect of sequential immunization of balb/c mice, with herpesvirus amplicon vector-based vaccines combined with protein-based vaccin ...201425458492
prevalence survey of selected bovine pathogens in water buffaloes in the north region of brazil.although the largest buffalo herd in the occident is in the north region of brazil, few studies have been conducted to assess the prevalence of selected parasitic diseases in buffalo herd. the present study was therefore conducted to investigate the epidemiological of toxoplasma gondii, neospora caninum, anaplasma marginale, babesia bigemina, and babesia bovis in water buffaloes in the north region of brazil. a total of 4796 buffalo blood samples were randomly collected from five provinces and s ...201424563780
identification and characterization of a trypanosoma congolense 46 kda protein as a candidate serodiagnostic antigen.trypanosoma congolense is a major livestock pathogen in africa, causing large economic losses with serious effects on animal health. reliable serodiagnostic tests are therefore urgently needed to control t. congolense infection. in this study, we have identified one t. congolense protein as a new candidate serodiagnostic antigen. the 46.4 kda protein (tcp46, gene id: tcil3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. the complete nucleotide sequences of ...201424492330
a proline racemase based pcr for identification of trypanosoma vivax in cattle blood.a study was conducted to develop a trypanosoma vivax (t. vivax) specific pcr based on the t. vivax proline racemase (tvprac) gene. forward and reverse primers were designed that bind at 764-783 bp and 983-1002 bp of the gene. to assess its specificity, tvprac pcr was conducted on dna extracted from different haemotropic pathogens: t. vivax from nigeria, ethiopia and venezuela, t. congolense savannah type, t. brucei brucei, t. evansi, t. equiperdum, t. theileri, theileria parva, anaplasma margina ...201424416292
risk factors for anaplasmosis in dairy cows during the peripartum.anaplasma marginale is endemic in tropical and subtropical areas around the world. some studies have suggested that cows during peripartum may present a transient immunosuppression state and development of clinical signs of anaplasmosis. the aim of this study was to investigate the relationship between some risk factors and the seroprevalence of a. marginale in dairy cows during peripartum in rio de janeiro, brazil. the risk factors analyzed in association with the prevalence of antibodies again ...201424307390
study of vitellogenin in boophilus annulatus tick larvae and its immunological aspects.boophilus annulatus is an important one-host tick in the mediterranean regions and iran. it can transmit the babesia bigemina, babesia bovis and anaplasma marginale to cattle. nowadays, immunization programs by tick proteins is one of the potential methods for the control and prevention of tick infestations. therefore, the characterization and identification of various tick proteins are necessary. vitellogenin is a precursor of vitellin that is produced in mid gut cells and fat bodies in ticks. ...201425382465
first evaluation of an outbreak of bovine babesiosis and anaplasmosis in southern brazil using multiplex pcr.outbreaks of tick-borne disease cases in santa catarina, brazil are known, but the presence of the pathogen dna has never been determined. in this study, the first survey of anaplasma marginale, babesia bigemina, and babesia bovis dna on blood samples of 33 cattle from an outbreak in ponte alta municipality, santa catarina, brazil, has been carried out. a multiplex pcr detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. the most preval ...201425352699
infection of water buffalo in rio de janeiro brazil with anaplasma marginale strains also reported in cattle.anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. the importance of water buffalo in the world economy is increasing. in addition, while water buffalo may serve as a reservoir host for a. marginale, the susceptibility of this host for a. marginale cattle strains in brazil has not been reported. the major surface protein 1 alpha (msp1α) gene has been shown to be a stable genetic marker for iden ...201425260335
the dynamics of erythrocyte infection in bovine anaplasmosis: a flow cytometry-based analysis.anaplasma marginale (a. marginale) is an obligate intracellular bacterium that infects bovine erythrocytes causing extravascular hemolysis and anemia. in the present work, we combine syto16 labeling of parasitized cells with the statistical power of flow cytometry to study the evolution of erythrocyte infection during bovine anaplasmosis.201425219763
low genetic diversity associated with low prevalence of anaplasma marginale in water buffaloes in marajó island, brazil.the rickettsia anaplasma marginale is the etiologic agent of bovine anaplasmosis, an important tick-borne disease affecting cattle in tropical and subtropical regions of the world. in endemic regions, the genetic diversity of this pathogen is usually related to the high prevalence of the disease in cattle. the major surface protein 1 alpha (msp1a) has been used as a marker to characterize the genetic diversity and for geographical identification of a. marginale strains. the present study reports ...201425108778
infectious pathogens potentially transmitted by semen of the black variety of the manchega sheep breed: health constraints for conservation purposes.conservation of genetic resources from endangered breeds may be conducted through germinal banks. preservation of healthy samples is paramount to avoid preserving pathogens shed with germinal products. the black variety of manchega sheep (bms), and endangered breed endemic to south-central spain, is the subject of a conservation program; a germinal bank has been recently established. however, several pathogens circulating in bms flocks may be shed with semen and threaten bms preservation. theref ...201425066603
epidemiology and evolution of the genetic variability of anaplasma marginale in south africa.bovine anaplasmosis caused by infection of cattle with anaplasma marginale has been considered to be endemic in south africa, an assumption based primarily on the distribution of the tick vectors of a. marginale and serological studies on the prevalence of anaplasmosis in limpopo, free state, and north west. however, molecular evidence of the distribution of anaplasmosis has only been reported in the free state province. in order to establish effective control measures for anaplasmosis, epidemio ...201425024014
development and assessment of a latex agglutination test based on recombinant msp5 to detect antibodies against anaplasma marginale in cattle.the recombinant protein msp5 has been established as an important antigen for serological diagnosis of anaplasma marginale by enzyme-linked immunosorbent assay (elisa). however, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. the present study describes the standardization of a latex agglutination test (lat) to detect antibodies against a. marginale based on recombinant ...201424948931
seroprevalence of anaplasma marginale in texas cattle.to our knowledge the seroprevalence of anaplasma marginale in texas has not been reported. the objective of this study was to estimate the point seroprevalence and spatial distribution of texas cattle persistently infected with a. marginale. this was a cross-sectional observational study examining serum collected from 12,000 adult cattle marketed in 23 selected texas auction markets during the second week of july 2011. a random subset of those cattle comprising 1835 individuals was evaluated for ...201424931130
use of percoll gradients to purify anaplasma marginale (rickettsiales: anaplasmataceae) from tick cell cultures.anaplasma marginale (rickettsiales: anaplasmataceae) is an obligate intracellular bacterium that multiplies exclusively within membrane-bound vacuoles in the cytoplasm of host cells. a number of a. marginale isolates can be propagated in the ixodes scapularis ide8 tick cell line, which provides a reliable source of antigens for a wide variety of studies. however, because of its intracellular nature, separation of bacteria from host cell materials remains an important constraint for researchers. ...201424877977
tabanids: neglected subjects of research, but important vectors of disease agents!tabanids are nuisance pests for people and livestock because of their painful and irritating bite, persistent biting behavior, and blood ingestion. about 4400 tabanid species have been described; they are seasonally present in all kinds of landscapes, latitudes, and altitudes. high populations have a significant economic impact on outdoor activities, tourism, and livestock production. tabanids are also vectors of animal disease agents, including viruses, bacteria and parasites. however, tabanids ...201424727644
evaluation of humoral and cellular immune response of balb/c mice immunized with a recombinant fragment of msp1a from anaplasma marginale using carbon nanotubes as a carrier molecule.bovine anaplasmosis is a disease caused by the intraerythrocytic rickettsia anaplasma marginale. surface proteins (msps) of a. marginale are important in the interaction of the pathogen with the host and constitute potential vaccine targets against this pathogen. currently, there is no commercial inactivated vaccine against bovine anaplasmosis that can generate a protective immune response that effectively prevents the development of clinical disease. the objective of this study was to evaluate ...201424606864
molecular and serological prevalence of anaplasma marginale in water buffaloes in northern brazil.bovine anaplasmosis, caused by anaplasma marginale, occurs in tropical and subtropical regions throughout the world and is a major constraint on cattle production in many countries. approximately 60% of the buffalo herds in south america are located in northern brazil. however, compared with the research on cattle, research on buffaloes has been neglected. therefore, the present study was conducted to investigate the distribution of a. marginale in water buffaloes in northern brazil. a total of ...201424246707
immunogenicity of hypothetical highly conserved proteins as novel antigens in anaplasma marginale.anaplasma marginale is a tick-transmitted gram-negative intraerythrocytic bacterium and the etiological agent of bovine anaplasmosis. even though considerable research efforts have been undertaken, anaplasmosis vaccine development remains a challenging field. outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. thus, the identification of outer-membrane antigens represents a major goal in the development ...201424126603
effectiveness of enrofloxacin for the treatment of experimentally-induced bovine anaplasmosis.four groups of six holstein calves were inoculated with 3.6 × 10(7) erythrocytes parasitized with anaplasma marginale. the criteria for treatment of calves were increasing a. marginale rickettsemia and 30% reduction of baseline packed cell volume (pcv) of each animal. group 1 (g1) received 7.5 mg.kg(-1) of enrofloxacin in a single dose; group 2 (g2) 7.5 mg.kg(-1) of enrofloxacin twice every three days; group 3 (g3) 20 mg.kg(-1) of long-acting oxytetracycline in a single dose; and group 4 (g4) a ...201422534942
control of anticoagulant treatment. 20144187840
two forms of haemophilia. 20144175422
bile duct varices.the diagnosis of biliary duct varices and portal vein occlusion should be considered when nodular or notched defects in the wall of the biliary duct system are shown by cholangiography or when pedunculated vascular structures in the bile ducts are seen at surgery. we present two cases of common hepatic and common bile duct varices due to portal vein occlusion.20144038065
gonadotropin secretion dynamics in patients with klinefelter's syndrome in relation to age.in 28 chromatin-positive patients distributed into age-decade lots, the circulating levels of gonadotropins as shown by radioimmunoassay (ria), were higher than normal. fsh was especially high while lh had significant fluctuations between the lots. a hypothalamic determinism for these anomalies is suggested.20143937217
[malignant melanoma: histological findings and their significance for prognosis]. 20144334609
[comparative studies on the mucopolysaccharide content of layers of the wall of the aorta with reference to atherosclerosis, age and sex]. 20144249288
[s.i. spasokukotskiĭ in saratov]. 20144249317
blood pressure and the menstrual cycle.a retrospective analysis of data from 207 non-pregnant premenopausal women showed that the mean level of systolic blood pressure varied with the stage of the menstrual cycle, being higher on days 17-26, the part of the luteal phase during which the peak of progestogen levels develops, than during the luteal phase as a whole, and significantly higher than the mean for all other days of the cycle. the mean levels were 125.4 mmhg (se 1.76) for days 17-26, 122.5 (se 1.25) for days 15-28 and 120.1 (s ...20144052342
prevention of myocardial damage in acute myocardial ischemia by early treatment with intravenous streptokinase.we evaluated the effectiveness of early intravenous administration of 750,000 units of streptokinase in 53 patients with acute myocardial ischemia treated by a mobile-care unit at home (9 patients) or in the hospital (44 patients). treatment was begun an average (+/- s.d.) of 1.7 +/- 0.8 hours from the onset of pain. non-q-wave infarctions developed subsequently in eight patients, whereas all the others had typical q-wave infarct patterns. in 81 per cent of the patients the infarct-related arter ...20144058533
slow myosin heavy chain isozyme in nemaline myopathy.muscle biopsies from two sporadic cases of congenital nemaline myopathy were examined for myosin heavy chain composition. electrophoresis of congenital nemaline myopathy (cnm) muscle myosin in sds-5% polyacrylamide gels gave rise to a single heavy chain band, with a migration rate and antigenic properties identical to that of the adult slow form, as demonstrated by western blot techniques and by using specific antibody. immunofluorescent studies indicate that cnm muscle fibers, including the mos ...20144022386
acute myelomonocytic leukemia in a patient with hodgkin's disease. 20144198910
[diabetic lipoidic necrobiosis. histology and histochemistry]. 20144107163
[centric relation and the stomatognathic system]. 20143868791
a history of theatre swabs and packs. 20143900739
interaction between perinatal brain damage and processes of normal brain development. ultrasonographic and neurodevelopmental study in the first year of life.a systematic ultrasonographic study and a prospective neurodevelopmental assessment were carried out in a population of high-risk neonates. in group a (82 preterm infants of 34 weeks gestation or less), periventricular-intraventricular hemorrhage (pvh) and periventricular leukomalacia (pvl) were the commonest lesions. the association of pvh, pvl and ventricular dilatation had a variable outcome and the prognosis was found to be poorer in the presence of diffuse or extensive pvl. in group b (115 ...20143896746
congenital single incisor. 20154528486
[cephalometric analysis of the effect of the chin sling]. 20154534519
[accidents in children in osterund, 1971]. 20154486451
[spectroscopic study of some substituted n-(2-diethylamino-ethyl) benzamides]. 20154774835
an enzymatic determination of ammonia in biological fluids. 20154284306
[experience in a public health nurse's home visiting]. 20154487407
oral and maxillo-facial surgery coming to age in puerto rico. 20154526656
[occupational medicine problems of an aviation physician]. 20154701759
[occupational medicine problems of an aviation physician]. 20154701759
detection and assessment of risk factors associated with natural concurrent infection of trypanosoma evansi and anaplasma marginale in dairy animals by duplex pcr in eastern punjab.duplex pcr consisting of two primer sets within a single mixture for the simultaneous detection of anaplasma marginale and trypanosoma evansi was standardized and employed on 219 blood samples collected from cattle (165) and buffaloes (54) from eastern punjab to evaluate the status of concurrent infection and associated risk factors. the reaction produced 257- and 407-bp amplification products targeting repetitive nucleotide sequence of t. evansi and msp1β gene of a. marginale, respectively. the ...201525358242
first molecular survey and novel genetic variants' identification of anaplasma marginale, a. centrale and a. bovis in cattle from tunisia.few data are available about the presence and distribution of anaplasma species in cattle in north african countries. in this study prevalence, co-infections, risk factors and genetic diversity of anaplasma species were evaluated in bovines from northern tunisia. a total of 232 cattle from 36 randomly selected farms in three tunisian localities were investigated for the presence of anaplasma species in blood by real-time pcr and/or nested pcr. overall infection rates of anaplasma spp., anaplasma ...201526117444
bovine anaplasmosis in turkey: first laboratory confirmed clinical cases caused by anaplasma phagocytophilum.anaplasma species are obligate intracellular rickettsial pathogens that affect the health of humans and other animals. clinical cases of anaplasmosis caused by anaplasma phagocytophilum were evaluated, and the frequency of bovine anaplasma species was determined in cattle. blood samples and thin blood smears were collected from 10 cattle exhibiting clinical signs of tick-borne fever. in addition, blood samples were collected from 123 apparently healthy cattle from the same area. dna was screened ...201526051478
development of a new pcr-based assay to detect anaplasmataceae and the first report of anaplasma phagocytophilum and anaplasma platys in cattle from algeria.bovine anaplasmosis is a hemoparasitic disease considered as a major constraint to cattle production in many countries. this pathology is at least partially caused by anaplasma phagocytophilum, anaplasma marginale, anaplasma centrale, and anaplasma bovis. the global threat and emergence of these species in animals require the reliable identification of these bacteria in animal samples. in this study, we developed a new qpcr tool targeting the 23s rrna gene for the detection of anaplasmataceae ba ...201525748051
characterization of two strains of anaplasma marginale isolated from cattle in rio de janeiro, brazil, after propagation in tick cell culture.ide8 tick cell cultures have been used for the isolation and propagation of several isolates of anaplasma marginale. the genetic heterogeneity of a. marginale strains in cattle is diverse in endemic regions worldwide and the analyses of msp1α (major surface protein 1 alpha) gene sequences have allowed the identification of different strains. this study reports the isolation and propagation of two new isolates of a. marginale in ide8 cells from blood of two cattle and their morphological and mole ...201525468764
species distribution modelling for rhipicephalus microplus (acari: ixodidae) in benin, west africa: comparing datasets and modelling algorithms.rhipicephalus microplus is one of the most widely distributed and economically important ticks, transmitting babesia bigemina, b. bovis and anaplasma marginale. it was recently introduced to west africa on live animals originating from brazil. knowing the precise environmental suitability for the tick would allow veterinary health officials to draft vector control strategies for different regions of the country. to test the performance of modelling algorithms and different sets of environmental ...201525466219
molecular biological identification of babesia, theileria, and anaplasma species in cattle in egypt using pcr assays, gene sequence analysis and a novel dna microarray.in this preliminary study, a novel dna microarray system was tested for the diagnosis of bovine piroplasmosis and anaplasmosis in comparison with microscopy and pcr assay results. in the dakahlia governorate, egypt, 164 cattle were investigated for the presence of piroplasms and anaplasma species. all investigated cattle were clinically examined. blood samples were screened for the presence of blood parasites using microscopy and pcr assays. seventy-one animals were acutely ill, whereas 93 were ...201525591406
development of a multilocus sequence typing scheme for the study of anaplasma marginale population structure over space and time.bovine anaplasmosis caused by anaplasma marginale is a worldwide disease prevalent in tropical and subtropical regions where rhipicephalus microplus is considered the most significant biological vector. molecular markers previously applied for a. marginale typing are efficient for isolate discrimination but they are not a suitable tool for studying population structure and dynamics. here we report the development of an mlst scheme based on the study of seven genes: dnaa, ftsz, groel, lipa, reca, ...201525550150
anaplasma marginale and anaplasma phagocytophilum: rickettsiales pathogens of veterinary and public health significance.anaplasma marginale and anaplasma phagocytophilum are the most important tick-borne bacteria of veterinary and public health significance in the family anaplasmataceae. the objective of current review is to provide knowledge on ecology and epidemiology of a. phagocytophilum and compare major similarities and differences of a. marginale and a. phagocytophilum. bovine anaplasmosis is globally distributed tick-borne disease of livestock with great economic importance in cattle industry. a. phagocyt ...201526346451
pathological observations on clinical anaplasma marginale infection in cattle.gross and histopathological changes were recorded in a pregnant cattle died of clinical anaplasmosis, a tick transmitted economically important disease caused by anaplasma marginale. grossly emaciated carcass along with pale visible mucous membranes and pale serosal surface, splenomegaly and hepatomegaly was observed. microscopically, in lungs variable extend of interstitial pneumonia, emphysema along with infiltration of mononuclear cells was seen. spleen showed extensive increase in red pulp a ...201526345059
primary structural variation in anaplasma marginale msp2 efficiently generates immune escape variants.antigenic variation allows microbial pathogens to evade immune clearance and establish persistent infection. anaplasma marginale utilizes gene conversion of a repertoire of silent msp2 alleles into a single active expression site to encode unique msp2 variants. as the genomic complement of msp2 alleles alone is insufficient to generate the number of variants required for persistence, a. marginale uses segmental gene conversion, in which oligonucleotide segments from multiple alleles are recombin ...201526259814
low temperature affects cattle tick reproduction but does not lead to transovarial transmission of anaplasma marginale.anaplasma marginale is an obligate intracellular pathogen that infects the erythrocytes of calves, causing bovine anaplasmosis. this rickettsia is biologically transmitted by several species of ticks. in tropical and subtropical regions of the world, rhipicephalus microplus is the main vector. due to their mobility and longevity, the adult males play an important role in the transmission of a. marginale to calves. some studies have demonstrated that a. marginale can be intrastadially and interst ...201526255094
bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick subolesin-anaplasma marginale msp1a chimeric antigen.ticks are vectors of diseases that affect humans and animals worldwide. tick vaccines have been proposed as a cost-effective and environmentally sound alternative for tick control. recently, the rhipicephalus microplus subolesin (sub)-anaplasma marginale msp1a chimeric antigen was produced in escherichia coli as membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. in this research, lipidomics and proteomics characterization of the e. coli membrane-b ...201526219233
molecular characterization of anaplasma marginale in ticks naturally feeding on buffaloes.anaplasma marginale is the most prevalent pathogen transmitted by ticks in cattle in tropical and subtropical regions of the world. however, the tick species involved in the transmission of a. marginale in buffaloes in brazil have not been identified. the objective of the present study was to determine the presence of a. marginale in ticks parasitizing water buffaloes. a total of 200 samples of rhipicephalus microplus, dermacentor nitens, amblyomma cajennense, and amblyomma maculatum were collec ...201526209411
molecular epidemiological survey and genetic analysis of vector-borne infections of cattle in luzon island, the philippines.in the philippines, vector-borne disease is one of the important problems in the livestock industry. to elucidate the epidemiology of vector-borne diseases in cattle on luzon island, the philippines, the prevalence of five protozoan agents was assessed by polymerase chain reaction. out of the 339 samples, 324 (95.5%), 154 (45.4%), 209 (61.6%), 140 (41.3%), and 2 (0.6%) were positive for anaplasma marginale, babesia bigemina, babesia bovis, theileria spp., and trypanosoma evansi infections, respe ...201526138152
molecular detection of novel anaplasmataceae closely related to anaplasma platys and ehrlichia canis in the dromedary camel (camelus dromedarius).serological surveys have confirmed anaplasma marginale and anaplasma phagocytophilum infections in dromedary camels, but molecular surveys and genetic characterisation of camel-associated anaplasma species are lacking. in this study, we detected tick-borne anaplasmataceae in 30 of 100 (30%) healthy dromedary camels screened using a combined 16s rrna-groel pcr-sequencing approach. nucleotide sequencing confirmed anaplasmataceae genome presence in 28 of the 33 16s rrna pcr-positive samples, with t ...201526096752
correction: association of anaplasma marginale strain superinfection with infection prevalence within tropical regions. 201526020787
genetic diversity and molecular phylogeny of anaplasma marginale studied longitudinally under natural transmission conditions in rio de janeiro, brazil.anaplasma marginale is the most prevalent tick-borne pathogen in cattle in tropical and subtropical regions of the world. major surface protein 1a (msp1a) has been found to be a stable genetic marker for identifying a. marginale isolates within geographical regions. it is conserved in cattle during infection and tick-borne transmission of the pathogen. the aim of the present longitudinal study was to determine occurrences of genetic diversity associated with high prevalence of a. marginale under ...201525985719
pharmacological characterization of a tyramine receptor from the southern cattle tick, rhipicephalus (boophilus) microplus.the southern cattle tick (rhipicephalus (boophilus) microplus) is a hematophagous external parasite that vectors the causative agents of bovine babesiosis or cattle tick fever, babesia bovis and b. bigemina, and anaplasmosis, anaplasma marginale. the southern cattle tick is a threat to the livestock industry in many locations throughout the world. control methods include the use of chemical acaricides including amitraz, a formamidine insecticide, which is proposed to activate octopamine receptor ...201525958152
molecular and immunological characterization of three strains of anaplasma marginale grown in cultured tick cells.anaplasma marginale is an economically important tick-borne pathogen of cattle that causes bovine anaplasmosis. a wide range of geographic strains of a. marginale have been isolated from cattle, several of which have been characterized using genomics and proteomics. while many of these strains have been propagated in tick lines, comparative analyses after propagation in tick cells have not been reported. the overall purpose of this research therefore was to compare the degree of conservation of ...201525943785
loss of immunization-induced epitope-specific cd4 t-cell response following anaplasma marginale infection requires presence of the t-cell epitope on the pathogen and is not associated with an increase in lymphocytes expressing known regulatory cell phenotypes.we have shown that in cattle previously immunized with outer membrane proteins, infection with anaplasma marginale induces a functionally exhausted cd4 t-cell response to the a. marginale immunogen. furthermore, t-cell responses following infection in nonimmunized cattle had a delayed onset and were sporadic and transient during persistent infection. the induction of an exhausted t-cell response following infection presumably facilitates pathogen persistence. in the current study, we hypothesize ...201525924762
anaplasma marginale major surface protein 1a: a marker of strain diversity with implications for control of bovine anaplasmosis.classification of bacteria is challenging due to the lack of a theory-based framework. in addition, the adaptation of bacteria to ecological niches often results in selection of strains with diverse virulence, pathogenicity and transmission characteristics. bacterial strain diversity presents challenges for taxonomic classification, which in turn impacts the ability to develop accurate diagnostics and effective vaccines. over the past decade, the worldwide diversity of anaplasma marginale, an ec ...201525802034
molecular and serological in-herd prevalence of anaplasma marginale infection in texas cattle.bovine anaplasmosis is an infectious, non-contagious disease caused by the rickettsial pathogen anaplasma marginale (a. marginale). the organism has a global distribution and infects erythrocytes, resulting in anemia, jaundice, fever, abortions and death. once infected, animals remain carriers for life. the carrier status provides immunity to clinical disease, but is problematic if infected and naïve cattle are comingled. knowledge of infection prevalence and spatial distribution is important in ...201525732914
[detection of anaplasma / ehrlichia species of cattle and ticks in aydın region].the aim of this study is to detect the anaplasma/ehrlichia species of cattle and ticks and to provide knowledge on the prevalence of these species during sampling periods.201526809916
outbreak of anaplasmosis associated with the presence of different anaplasma marginale strains in dairy cattle in the states of são paulo and goiás, brazil.the present study reports the genetic diversity of anaplasma marginale during anaplasmosis outbreaks in rural properties of the states of goiás and são paulo, brazil. mortality rates of 3.5% (37/1,050) in calves, 4.7% (45/954) in heifers and 1.1% (25/2,200) in lactating cows were observed in a cattle herd of the municipality of mambaí, state of goiás, central-western brazil. in a cattle herd from the municipality of lins, state of são paulo, in southeastern brazil, none of the animals died, desp ...201526648009
[cardiothyrotoxicosis]. 20154657071
[assistance in functional rehabilitation of hemiplegic patients]. 20154625931
replantation of upper extremities. 20154607018
symposium on effects of weather and climate on plants and trees. 20154774779
[one-stage surgical treatment of complete transposition of great vessels associated with ventricular heart septal defects]. 20154536383
utilization of non-protein nitrogen in the production of animal protein. 20154598416
[reimplantation of extremities or parts thereof]. 20154486961
[continuing education in pediatrics in the 1980's]. 20154825280
the heritability of serum lipoprotein and lipid concentrations. a twin study. 20154474930
[kinetic manifestations of conformational changes in enzymes]. 20154469585
[contraception and epilepsy]. 20154447279
blind intramedullary nailing for tibial fractures. 20154430169
[ultrastructural study of the blue histocytes in a case of idiopathic lipidosis of the spleen with ceroid overload (author's transl)]. 20154355815
investigation of tick-borne bacteria (rickettsia spp., anaplasma spp., ehrlichia spp. and borrelia spp.) in ticks collected from andean tapirs, cattle and vegetation from a protected area in ecuador.ixodid ticks play an important role in the transmission and ecology of infectious diseases. information about the circulation of tick-borne bacteria in ticks is lacking in ecuador. our aims were to investigate the tick species that parasitize andean tapirs and cattle, and those present in the vegetation from the buffer zone of the antisana ecological reserve and cayambe-coca national park (ecuador), and to investigate the presence of tick-borne bacteria.201525616567
[preleukemic state with symptoms of aplastic anemia]. 20154674957
[suicide and attempted suicide. trends, statistics, prevention, help]. 20154595018
emergence of oriental theileriosis in cattle and its transmission through rhipicephalus (boophilus) microplus in assam, india.the aim of the present study was to investigate the presence of theileria in blood samples of crossbred and indigenous adult cows raised under unorganized small scale farming system in a babesia and anaplasma endemic geographical area from assam, india and to see its transmission through rhipicephalus (boophilus) microplus ticks.201527047205
[the ph in the duodenum, physiological and pathophysiological significance]. 20154574146
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