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human and animal bite wounds.bite wounds account for 1 percent of emergency department visits. most bites occur on the hands. human bites cause serious injury more often than animal bites do. pasteurella multocida is found in about 25 percent of dog bites and a higher percentage of cat bites. alpha-hemolytic streptococci are the most frequent isolates from human bite wounds. anaerobic bacteria are commonly found in all types of infected bites.19873604843
studies on pasteurella multocida of birds. second communication: colonial and biochemical characteristics in association with virulence to mice. 19873606314
studies on pasteurella multocida of birds. third communication: serological typing of pasteurella multocida strains isolated from chickens and turkeys. 19873606315
studies on pasteurella multocida of birds. fourth communication: a new polyvalent oil-adjuvant vaccine. 19873606316
diurnal changes in coronary blood flow in conscious dogs.the purpose of the present study was to evaluate potential diurnal changes in resting coronary blood flow under conditions of comparable myocardial oxygen requirements. we studied 21 conscious, resting dogs instrumented for the measurement of coronary flow, left ventricular pressure, and regional myocardial segment length. recordings were taken in the early morning and late afternoon. in the afternoon, left circumflex coronary blood flow was 12.8% higher than in the morning, without detectable c ...19873608130
relations between brain and cognitive development.goldman-rakic reports important new data on cortical development in rhesus monkeys. some of her findings, especially concurrent cortical synaptogenesis, may be related to cognitive capacities that develop in infancy. the developmental pattern of concurrent synaptogenesis in rhesus is consistent with a straightforward model of relations between brain and cognitive development: concurrent synaptogenesis is hypothesized to lay the primary cortical foundation for a series of developmental levels in ...19873608642
electron microscopic visualization of capsular material of pasteurella multocida types a and d labeled with polycationic ferritin.the presence of capsular material on cells of pasteurella multocida types a and d was determined by transmission electron microscopy after polycationic ferritin labeling. the capsule of agar-grown isolates of p. multocida type a was thick (70 to 90 nm) and regular, whereas that of type d isolates was thinner (20 to 30 nm) and irregular. such layers were seen on cells from 4- to 6-h broth cultures, but cells from older cultures (12 to 18 h) had very little cell-associated capsular material. our d ...19873611019
isolation and characterization of temperature-sensitive mutants of pasteurella multocida capsular serotype d strain. 19873613353
[experimental studies on the effect of chronic aerogenous toxic gas stress in suckling pigs using different concentrations of ammonia. i. clinical picture of nh3-exposed suckling pigs under the conditions of experimental pasteurella multocida infection with and without thermomotor stress]. 19873618010
[pasteurella multocida as the agent of atrophic rhinitis: significance of dermonecrotoxin in the suckling mouse test and in vitro demonstration of adherence properties]. 19873618013
fate of pasteurella multocida in the blood vascular system of turkeys following intravenous inoculation: comparison of an encapsulated, virulent strain with its avirulent, acapsular variant.a virulent, encapsulated strain of pasteurella multocida was compared with a spontaneously arising, avirulent acapsular variant following injection into the bloodstream of 14-week-old turkeys. neither strain was detectable in the blood by 1 hour, but they reappeared 4 hours postinoculation in approximately equal numbers. the concentration of both strains increased with time, but the virulent strain reached concentrations 100,000-fold higher than the avirulent strain 15-24 hours after inoculation ...19873619818
comparison of vaccination protocols of broiler breeder hens for pasteurella multocida utilizing enzyme-linked immunosorbent assay and virulent challenge.a commercial enzyme-linked immunosorbent assay (elisa) to detect serological response to vaccination and virulent challenge with type 1 (x-73) pasteurella multocida was used to determine the best vaccination protocol for broiler breeders against fowl cholera. birds vaccinated twice, at 10 and 19 weeks of age, with the avirulent clemson university (cu) strain both times, with a commercial bacterin first and the cu strain second, or with the cu strain first and bacterin second had the highest surv ...19873619819
avian cholera in a gyrfalcon (falco rusticolus).a 2-year-old female gyrfalcon (falco rusticolus) was being used to hunt ducks in southeastern wyoming during an outbreak of avian cholera in waterfowl and wild turkeys. while out overnight, the falcon consumed a bird. within 24 hours the falcon was anorectic, and it was found dead approximately 48 hours following ingestion of wild prey. gross and microscopic lesions were typical of avian cholera, and pasteurella multocida serotype 1 was isolated from tissues of the falcon.19873619832
stimulation of bone resorption by inflamed nasal mucosa, dermonecrotic toxin-containing conditioned medium from pasteurella multocida, and purified dermonecrotic toxin from p. multocida.the effects of inflamed nasal mucosa from pigs with atrophic rhinitis (ar), cell extract from bordetella bronchiseptica, conditioned medium from pasteurella multocida, and purified dermonecrotic toxin (dnt) from p. multocida on mouse fetal long bones in organ culture were studied. inflamed nasal "ar mucosa" stimulated the release of 45ca from prelabeled cultures, while histologically the formation of calcified matrix was impaired as well. b. bronchiseptica cell extract only transiently increased ...19873623695
role of exposure parameters in toxicity of aerosolized diesel fuel in the rat.sprague-dawley rats were exposed to aerosolized diesel fuel (adf) to determine the potential health effects in military troops during exposure to this visual obscurrant. acute range finding studies were performed to estimate exposure conditions at which minimal (less than 1%) mortality would occur. this information was used in the design of a repeated exposure study to test the relative significance of the frequency of exposures, the duration of exposure, and aerosol concentration in any toxicit ...19873624787
thymus-derived stromal cell lines.we derived stromal cell lines from mouse thymus using methods previously established for bone marrow stroma. two main morphologically distinct groups of cell strains emerged: epithelioid and mixed fibroblast-macrophage. transmission electron microscopy revealed frequent junctional-complex formations between adjacent cells, a feature that characterized almost all of the thymus stromal lines, but was confined to only one of the five distinct subtypes of cell lines from bone marrow. in contrast to ...19873624917
tickborne diseases in georgia, 1986. 19873625055
the effect of venous blood stream cooling on survival of bacterially infected rabbits.the effect of physical cooling on the mortality rate of rabbits infected with pasteurella multocida was investigated. rabbits were cooled for 48 hours after bacterial injection by passing cold fluid through small hollow metal cuffs which had been surgically implanted around the abdominal vena cavae of rabbits. the average body temperatures of the rabbits during the 24-hour period after the intravenous injection of live pasteurella multocida was 40.92 +/- 0.20 degrees c in control rabbits and 38. ...19873627972
[results of experimental infection of calves with pasteurella multocida and pasteurella haemolytica]. 19873632244
pyogenic osteomyelitis of the occiput, the atlas, and the axis. a report of five cases.pyogenic osteomyelitis rarely affects the first and second cervical vertebrae, and when it does it can progress to abscess formation, compressing the spinal cord. if the process is unrecognized, it can be fatal. the cases of five patients are reported. two patients were treated by anterior débridement and posterior cervical-occipital arthrodesis; one, by transoral drainage; one, by posterior cervical-occipital arthrodesis; and the fifth, by posterior atlanto-axial arthrodesis. the causative orga ...19873654699
impairment of the extraneuronal o-methylating system of isoproterenol by stop-flow ischemia in the perfused rat heart.effects of ischemia on the extraneuronal o-methylating system were investigated in the perfused rat heart. ischemia was produced by stopping of the perfusion for 30 min. isoproterenol (iso) was used as a model substrate for the extraneuronal uptake of catecholamines. the accumulation of iso when catechol-o-methyltransferase (comt) (ec 2.1.1.6) was inhibited by 3',4'-dihydroxy-2-methylpropiophenone (u-0521) was decreased by ischemia to about 50% of the control value. ischemia decreased the format ...19873656108
pasteurella multocida meningitis. 19873656328
refined structure of glutathione reductase at 1.54 a resolution.the crystal structure of human glutathione reductase has been established at 1.54 a resolution using a restrained least-squares refinement method. based on 77,690 independent reflections of better than 10 a resolution, a final r-factor of 18.6% was obtained with a model obeying standard geometry within 0.025 a in bond lengths and 2.4 degrees in bond angles. the final 2fo-fc electron density map allows for the distinction of carbon, nitrogen and oxygen atoms with temperature factors below about 2 ...19873656429
purification of a heat labile dermonecrotic toxin from culture fluid of pasteurella multocida.a highly pure heat-labile dermonecrotic toxin (dnt) of pasteurella multocida was isolated from bacterium-free broth culture fluid. the protocol for the isolation included the following steps: ammonium sulphate precipitation, gel filtration, ion exchange chromatography and preparative polyacrylamide gel electrophoresis (page). about 1 mg of purified dnt was recovered from 3 l of broth culture fluid. the final product was toxic for embryonic bovine lung (ebl) cells, lethal for mice, dermonecrotic ...19873660579
pasteurella multocida urinary tract infection. 19873660782
cat-bite peritonitis: pasteurella multocida peritonitis following feline contamination of peritoneal dialysis tubing.we report a case of a 55-year-old woman with end-stage renal disease undergoing intermittent peritoneal dialysis who developed peritonitis secondary to an infection with pasteurella multocida resulting from a cat bite or cat scratch penetrating her peritoneal dialysis tubing. this case points out the potential importance of household pets as a source of peritoneal contamination.19873661553
properties of in vitro nonenzymatically glycated plasma fibrinogens.nonenzymatic glycation of fibrinogen is species independent and depends only on the glucose concentration in the incubation mixture under selected in vitro conditions. an increased fibrin monomer aggregation in the presence of ca2+ ions and a decreased proteolytic susceptibility of nonenzymatically glycated fibrinogens may favour the development of thrombophilic states. blocking of lysine residues as well as restricted conformational changes induced by glucose attachment may be responsible for t ...19873666059
[fiberoptic bronchoscopy--a possibility for the diagnosis of pasteurella multocida pneumonia]. 19873669819
[experimental studies on the effect of chronic aerogenous toxic gas burden of suckling pigs with different ammonia concentrations. ii. the reaction of cellular and humoral infection defense mechanisms of nh3-exposed suckling pigs under the conditions of an experimental pasteurella multocida infection with and without thermomotor stress]. 19873673361
[biopharmaceutical evaluation of the rectal administration of mefenamic acid. iv. biological availability of mefenamic acid on the base of polyoxyethylene glycol with the addition of polysorbates]. 19873673627
pneumonia in pigs induced by intranasal challenge exposure with pseudorabies virus and pasteurella multocida.the interaction between pseudorabies virus (prv) and pasteurella multocida was investigated to determine whether single or combined infections result in pneumonia in 6- to 7-week-old pigs. the effect of the prv-p multocida challenge exposure on feed consumption, rate of gain, and extent of pneumonic lesions appeared dependent on the prv dose; however, pneumonic lesions were of bacterial pneumonia. pigs inoculated with a virulent strain of prv plus p multocida developed severe pneumonia, whereas ...19873674553
pathogenesis of pasteurella multocida in experimentally infected rabbits. 19873675119
low density lipoprotein accumulation by ptfe grafts in the rabbit aorta. autoradiographic-morphologic correlations. 19873675978
a potassium thiocyanate extract vaccine prepared from pasteurella multocida 3:a protects rabbits against homologous challenge.potassium thiocyanate extracts of a virulent pasteurella multocida 3:a rabbit isolate were prepared and used as a vaccine in rabbits. the extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, dna, and rna. the protein and lipopolysaccharide profiles of the extract were similar to those of the p. multocida cell membrane. rabbits were vaccinated intranasally (i.n.) or intramuscularly (i.m.) four times at 1- or 3-week intervals and challenged i.n. with the homologous p. mult ...19873679540
dietary substitution of methionine by its precursors: influence on body growth of rats. 19873679713
severe pleuritis associated with certain strains of pasteurella multocida in swine.pasteurella multocida was isolated from 2 farms on which grower or finisher pigs had problems of severe emaciation and high death loss (greater than 5%). at necropsy, the pigs had extensive suppurative pleuritis and pericarditis, with adhesions over the lung surface. on one farm, the pigs also had multiple lung abscesses. histologic findings included polymorphonuclear cell infiltration in bronchial and alveolar spaces, thickening of alveolar walls, pleuritis, and in some cases, abscesses. from a ...19873679971
correlation of agonist-induced phosphorylation of chick heart muscarinic receptors with receptor desensitization.we have determined whether the process of agonist-mediated phosphorylation of the muscarinic receptor correlates with the process of muscarinic receptor desensitization in chick cardiac tissue. exposure of ventricular slices to the agonist carbachol under conditions previously shown to lead to large increases in muscarinic receptor phosphorylation (kwatra, m. m., and hosey, m. m. (1986) j. biol. chem. 261, 12429-12432) resulted in decreased affinity of the muscarinic receptor for agonists. the a ...19873680252
development of nonreducible technetium-99m(iii) cations as myocardial perfusion imaging agents: initial experience in humans.a series of 15 nonreducible technetium-99m(iii) complexes of formula tr-[99mtcl(y)2]+ has been prepared by a general synthetic route based on reductive addition of y to the technetium-99m (99mtc) intermediate [99mtcl(o)]+. in these complexes, selected for potential use as myocardial imaging agents, l represents one of the two tetradentate schiff base ligands n,n'-ethylenebis(acetylacetone iminato), (en), or n,n'-propylene-1,2-bis(acetylacetone iminato), (pn), while y represents a monodentate pho ...19873681444
avian diuretic response to renal portal infusions of the mycotoxin citrinin.citrinin is a nephrotoxic mycotoxin produced by common molds. previous research has shown that citrinin causes increased urine flow, increased free water clearance, and increased sodium, potassium, and inorganic phosphate excretion. the present study was conducted to evaluate the dose-response effects of citrinin and to further evaluate previously reported phosphaturic effects of citrinin. ureteral urine was collected from anesthetized domestic fowl during a control period (30 min) and during un ...19873684854
atrophic rhinitis in goats in norway.the spontaneous occurrence of atrophic rhinitis in 12 of 49 goat herds in one area of norway is described. the clinical signs included nose bleeding, nasal discharge, sneezing and tender noses. pathologically, the macroscopic and histological findings resembled those found in pigs with atrophic rhinitis. bacteriological investigation of nasal swabs in five of the herds revealed toxigenic strains of pasteurella multocida in three of them. in four of the herds the clinical signs were seen in two o ...19873686794
the efficacy of sulbactam-ampicillin in the therapy of respiratory disease associated with ampicillin resistant pasteurella species in housed calves.sulbactam-ampicillin is a combination of sulbactam, a beta-lactamase inhibitor, and ampicillin, a broad spectrum beta-lactam antibiotic. the efficacy of sulbactam-ampicillin was evaluated in the treatment of calf respiratory disease associated with ampicillin-sensitive and ampicillin-resistant strains of pasteurella haemolytica and pasteurella multocida. treatment with sulbactam-ampicillin was compared with treatment with ampicillin alone in 123 friesian calves, between three and five weeks old, ...19873686802
distribution of immunoreactive inhibin like material in different regions of human prostate.distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).19873688474
occupational exposure to animals and antibodies against pasteurella multocida.the relation between occupational exposure to cattle and prevalence of antibodies against pasteurella multocida was evaluated in 680 workers. three groups of exposed workers in abattoirs and slaughterhouses (s), in industrial breeding (i), and in traditional breeding (t) were compared with control workers not exposed to cattle or chicken (c). the prevalence of antibodies against capsular antigen a determined by indirect haemagglutination was significantly higher in the exposed groups (s: 26.2%; ...19873689719
evaluation of quantum ii microbiology system for identification of gram-negative bacteria of veterinary origin.the ability of a rapid, semiautomated bacterial identification system, the quantum ii microbiology system (abbott laboratories, irving, tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. a total of 378 isolates were tested, including 298 organisms in the family enterobacteriaceae and strains representing acinetobacter sp., aeromonas sp., flavobacterium sp., pasteurella multocida, plesiomonas sp., and pseudomonas spp. of these isolates, 333 (88.1%) were co ...19873693539
hyperimmune serum from rabbits immunized with potassium thiocyanate extract of pasteurella multocida protects against homologous challenge.hyperimmune rabbit sera directed to the kscn extract of 3:a pasteurella multocida were characterized by enzyme-linked immunosorbent assay (elisa), presolubilized cell radioimmunoprecipitation, and immunoblotting analysis. the results showed that the hyperimmune serum had a very high titer of immunoglobulin g elisa antibody and a negligible immunoglobulin a elisa antibody, precipitated 10 different outer membrane protein antigens by radioimmunoprecipitation, and reacted to 10 different membrane v ...19873693546
a dot-immunobinding assay for the serodiagnosis of pasteurella multocida infection in laboratory rabbits.a dot-immunobinding assay was developed to detect serum igg specific for lipopolysaccharide of rabbit isolates of p. multocida. the assay detected serum igg as early as 1 week after experimental subclinical nasal infection, whereas 8 weeks were required to detect antibody by a gel diffusion precipitin test. the assay was more reliable than nasal cultures, in that up to 46% of 16 weekly nasal washings of some infected rabbits failed to yield p. multocida. the bacterial antigen (proteinase k diges ...19873695396
transmission of pasteurella multocida in rabbits.contact and airborne transmission of p. multocida in rabbits was evaluated in an artificially controlled environment. transmission by contact occurred more readily from rabbits with acute infections than from rabbits with chronic infections. however, airborne transmission to rabbits in adjacent cages did not occur.19873695397
serological classification of pasteurella multocida. 19873307116
[field application of the indirect fluorescent antibody test in malaria control]. 19873308159
pulmonary cryptococcosis in aids.cryptococcus neoformans is emerging as an important etiologic agent of disseminated infection in patients with the acquired immunodeficiency syndrome (aids). little attention has been placed on the pulmonary expression of this systemic infection. we report five patients with aids and cryptococcosis with primary pulmonary involvement. patients usually presented with fever, cough, dyspnea, and pleuritic chest pain. chest x-ray findings varied from localized and diffuse infiltration to lymphadenopa ...19873308348
[a personal view of the history of the genus yersinia].the first recorded experience australia had of the genus yersinia was the arrival in 1889 of a french expedition led by pasteur's nephew, dr. adrien loir. at that time australia was in the grips of an epidemic of rabbits, and loir's purpose was to eradicate the rabbits by means of fowl plague (pasteurella multocida). sadly, bureaucratic and political obstacles prevailed, and loir was never granted permission to release his biological control agent. alexander yersin had been tempted to join loir' ...19873311629
pasteurella multocida bacteremia: report of thirteen cases over twelve years and review of the literature.13 episodes of bacteremia caused by pasteurella multocida were seen in a general hospital during a 12-year period. all the patients had an underlying disease (77% had cirrhosis) and 2 were receiving chemotherapy for hematologic malignancy. there was a numerical preponderance of male patients (69%). in 5/13 cases a recent animal-derived trauma could be found. in the other cases the source of the infecting organism was thought to be endogenous (from patients' own pharyngeal commensal flora) or sec ...19873313679
clinical pharmacokinetics of five oral cephalosporins in calves.the minimal inhibitory concentrations (mic) of cephalexin, cephradine, cefaclor, cefatrizine and cefadroxil for salmonella species, escherichia coli and pasteurella multocida isolated previously from young calves were determined. the mic90 values for cephalexin, cephradine and cefadroxil ranged between 3.12 micrograms ml-1 and 12.5 micrograms ml-1, whereas those of cefatrizine and cefaclor were 3.12 micrograms ml-1 and 0.78 microgram ml-1, respectively. each drug was administered intravenously a ...19873317582
effect of acute renal failure on insulin disposition in the isolated perfused rat kidney.the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ...19873318499
effect of cyclooxygenase blockade on gas exchange and hemodynamics in pseudomonas pneumonia.acute bilateral pseudomonas aeruginosa pneumonia was induced in 10 anesthetized dogs, after which five dogs received intravenous indomethacin (2 mg/kg) (indomethacin group), whereas five others were infused with saline (2 ml/kg) (control group). plasma levels of 6-ketoprostaglandin f1 alpha(6-keto-pgf1 alpha) and thromboxane b2 (txb2), stable metabolites of prostacyclin (pgi2) and thromboxane a2 (txa2), respectively, were measured by radioimmunoassay. although txb2 levels were not different befo ...19873320011
fatal pasteurella multocida septicaemia following a cat bite in a man without liver disease.we report a fatal case of septicaemia caused by pasteurella multocida that followed a cat bite. the patient, a man aged 54 years, was not suffering from disease of the liver but had a history of ischaemic heart disease, non-insulin-dependent diabetes mellitus and rheumatoid arthritis which had been treated with steroids for 13 years.19873320211
a method for culturing the nasopharyngeal area of rabbits.a method for obtaining antemortem nasopharyngeal cultures is described. its usefulness for detecting the carrier state of pasteurella multocida in clinically normal, apparently healthy nasal-culture-negative rabbits is also discussed.19873320513
specific antibody response to pasteurella multocida.six patients with culturally proven pasteurella multocida infection were evaluated serologically. the infections were 1 foot abscess, 1 septicemia, 3 bronchitis and 1 bronchopneumonia. most of them were elderly women closely exposed to pets or domestic animals. the serotypes of the strains were determined in 5 cases (3 a3, 2 a7). specific antibodies against capsular and somatic antigens of p. multocida were determined by indirect hemagglutination and agglutination respectively. the antibodies we ...19872445019
galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons.galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ...19872446770
distribution of a monoclonal antibody-recognized protective protein immunogen on the outer membranes of pasteurella multocida rabbit isolates.the distribution of a monoclonal antibody (mab)-recognized protective protein immunogen on the outer membrane of 153 pasteurella multocida rabbit isolates was determined by dot blot (db) analysis. mab 1608 reacted with 36 (24%) of the 153 clinical isolates. the db-positive clinical isolates expressed capsular antigens a, d, and nontypable and somatic antigens 2, 3, 10, 12, 15, and nontypable. western blot (immunoblot) analysis with adsorbed and eluted mab 1608 confirmed that the antigenic determ ...19882457598
adherence of pasteurella multocida or bordetella bronchiseptica to the swine nasal epithelial cell in vitro.the interaction of bordetella bronchiseptica or pasteurella multocida with swine nasal epithelial cells was studied in vitro. the mean number of b. bronchiseptica organisms adhered per cell was about three times as high as that of p. multocida (p less than 0.01), and the adherence was specifically inhibited by the homologous antiserum prepared with the whole-cell antigen of each bacterium. the poor affinity of p. multocida to the swine nasal mucosa as compared with that of b. bronchiseptica was ...19883335403
formation of dna adducts and water-soluble metabolites of benzo[a]pyrene in human monocytes is genetically controlled.formation of dna adducts and of water-soluble metabolites was studied in monocytes of 86 first-degree relatives of 15 families. tests were performed with blood monocytes using (g-3h)-benzo[a]pyrene as a model pro-carcinogen. variance analysis revealed significantly higher inter-familial than intra-familial variations. from these data we conclude that the formation of dna adducts is genetically controlled. therefore the enhanced formation of benzo[a]pyrene dna adducts in lung cancer patients foun ...19883338869
expression of heme oxygenase gene in rat and human liver.developmental changes of microsomal heme oxygenase were studied. in human fetal liver, the enzyme activity was 8 times higher than that detected in the adult liver. on the other hand, adult livers contained 4 times more cytochrome p450 than fetal livers. elevated heme oxygenase activity in fetal liver was not due to modulators present in the microsomes. similar to human, rat fetal liver also contained high enzyme activity which appeared to be regulated at the transcriptional level. hybridization ...19883342043
turbinate osteoporosis in pigs following intranasal inoculation of purified pasteurella toxin: histomorphometric and ultrastructural studies.turbinate osteoporosis, induced by intranasal inoculation of purified toxin isolated from serotype d pasteurella multocida, was investigated in 3- to 5-week-old, caesarean-derived, colostrum-deprived, isolation-reared pigs. marked bilateral reduction in relative volume of trabecular bone occurred in osseous cores of turbinates of toxin-treated pigs relative to control pigs on post-inoculation day (p.i.d.) 3, 6, 9, 12, and 15. the fractional resorptive surface along turbinate bone was greater in ...19883344568
characterization of the dermal lesions induced by a purified protein from toxigenic pasteurella multocida.the dermonecrotic effect of purified pasteurella multocida toxin (pmt) was studied sequentially in guinea pigs and rats. the skin reaction was initially an acute inflammatory reaction, with edema and emigration of neutrophils and a few eosinophils and diapedesis of some erythrocytes. four hours after intracutaneous injection the vessels were congested and thrombocytes were focally attached to the endothelial wall. twenty-four h after the injection the inflammatory reaction appeared more severe a ...19883345248
pasteurella multocida vertebral osteomyelitis. 19883347190
development of turbinate lesions and nasal colonization by bordetella bronchiseptica and pasteurella multocida during long-term exposure of healthy pigs to pigs affected by atrophic rhinitis.natural transmission of atrophic rhinitis from pigs from a herd with an endemic atrophic rhinitis problem to pigs from a herd free of atrophic rhinitis was demonstrated. six replicates each with five pigs from the endemic atrophic rhinitis herd (group a) and five pigs from the atrophic rhinitis-free herd (group b) were housed together from 5 wk of age, with each replicate kept in isolation rooms maintained at optimal and controlled environmental conditions. three replicates each with six pigs/ro ...19883349398
preparation and characterization of viable epithelial cells from rabbit distal colon.a preparation of viable epithelial cells, suitable for transport studies, was prepared from rabbit distal colon. enzymatic digestion of scraped mucosa liberated a population of intact colonic glands that were dissociated into cells by gentle agitation in culture medium. metabolism of [14c]glucose was constant over 2 h and was inhibited 70% by 1 mm ouabain. cells were loaded with the trapped, ph-sensitive fluorescent dye 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxy methyl ester and lea ...19883354672
cellular defense of the avian respiratory system: influx and nonopsonic phagocytosis by respiratory phagocytes activated by pasteurella multocida.poultry have a very limited number of resident macrophages in the normal steady-state respiratory tract. thus, poultry must rely heavily on active migration of phagocytic cells to the lungs and air sacs in defending against respiratory pathogens. intratracheal administration of a live, apathogenic pasteurella multocida vaccine (choloral; clemson university strain) increased the number of avian respiratory phagocytes (arp; obtained by lavage of lungs and air sacs) within 24 h by 3 orders of magni ...19883356464
lack of in vitro efficacy of oral forms of certain cephalosporins, erythromycin, and oxacillin against pasteurella multocida.the in vitro susceptibility of human isolates of pasteurella multocida to oral antimicrobial agents from our current study and from a review of the literature suggests that dicloxacillin (oxacillin), erythromycin, clindamycin, cephalexin, cefaclor, and cefadroxil should not be used for empiric therapy of animal bite wounds. agents that were consistently active against p. multocida were penicillin, ampicillin, amoxicillin-clavulanic acid, tetracycline, minocycline, chloramphenicol, trimethoprim-s ...19883364944
posttraumatic pasteurella multocida meningitis.the patient described was immunologically compromised by multisystem trauma. pasteurella multocida was isolated from the respiratory tract and subsequently from the cerebrospinal fluid; direct spread apparently occurred by way of a basilar skull fracture. sepsis was absent. he was successfully treated but subsequently had hydrocephalus, which has not previously been reported to occur after p multocida meningitis. because of the opportunistic nature of this infrequent human commensal and the sign ...19883368822
[detection of antibodies to pasteurella multocida and pasteurella haemolytica in the serum of calves]. 19883369910
[the structure of lipopolysaccharides from pasteurella multocida]. 19883369912
adherence of bordetella bronchiseptica and pasteurella multocida to porcine nasal and tracheal epithelial cells.the ability of 19 different bordetella bronchiseptica isolates and 25 pasteurella multocida isolates to adhere in vitro to porcine nasal and tracheal epithelial cells was examined. it was found that b. bronchiseptica adhered well to upper respiratory tract cells. in contrast the number of p. multocida organisms which adhered was four to six times less than the number of b. bronchiseptica adherent organisms. this difference was statistically significant (p less than 0.0001). both microorganisms a ...19883370564
[a rare etiology of ascitic fluid infection in cirrhosis: pasteurella multocida]. 19883371604
identification of immunogenic outer membrane proteins of pasteurella multocida 3:a in rabbits.four groups of protective rabbit immune sera were used to identify pasteurella multocida outer membrane immunogens by a radioimmunoprecipitation procedure and western blot (immunoblot) analysis. these are rabbit hyperimmune sera against kscn extract of p. multocida (group 1) and rabbit immune sera against the kscn extract of p. multocida (group 2), the outer membrane of p. multocida (group 3), and live p. multocida cells (group 4). rabbits mounted an antibody response to 18 proteins found in the ...19883372019
mental models in risk assessment: informing people about drugs.one way to communicate about the risks of drugs is through the use of package inserts. the problems associated with this medium of informing patients have been investigated by several researchers who found that people require information about drugs they are using, including extensive risk information, and that they are willing to take this information into account in their usage of drugs. but empirical results also show that people easily misinterpret the information given. a conceptual framewo ...19883375502
occurrence of two phases of return to oestrus in sows on commercial units. 19883376405
pasteurella multocida meningitis following orbital exenteration. 19883377053
in vitro and in vivo activities of sedecamycin against treponema hyodysenteriae.sedecamycin (lankacidin a), one of the lankacidin-group antibiotics, showed potent activity against treponema hyodysenteriae. the mics of sedecamycin against 79 field isolates of t. hyodysenteriae ranged from 0.78 to 12.5 micrograms/ml, the mic for 90% of the strains tested (mic90) being 3.13 micrograms/ml. the protective and therapeutic effects of sedecamycin were compared with those of carbadox, tiamulin, and lincomycin against experimental infection with t. hyodysenteriae in mice. the protect ...19883377458
virulence of avian capsular serogroup b pasteurella multocida for turkey poults.two strains of capsular serogroup b pasteurella multocida isolated from avian hosts (swan and turkey) were evaluated for virulence based on lethality for turkey poults. groups of poults were exposed intramuscularly to various concentrations of organisms of each strain. both strains were virulent. the strain isolated from a turkey was highly virulent: all exposed poults died in less than 24 hours, including those exposed to only 79 organisms. this highly virulent strain was neither highly invasiv ...19883382367
characterization of an avian cholera epizootic in wild birds in western nebraska.avian cholera killed an estimated 2500 birds in western nebraska and eastern wyoming from 28 november 1985 to late january 1986. wild mallards (anas platyrhynchos) suffered the most losses. other wild waterfowl, wild turkeys (meleagris gallopavo), a few domestic fowl, and a bald eagle (haliaeetus leucocephalus) also died. pasteurella multocida serotype 1 was the predominant isolate from these carcasses. cold, wet weather persisted throughout the outbreak, but daily losses in the flock of 50,000 ...19883382368
pasteurella multocida in wild mammals and birds in california: prevalence and virulence for turkeys.samples collected from the oropharynx of wild mammals and birds trapped on 36 turkey farms in california were evaluated for the presence of pasteurella multocida. a total of 966 animals were collected from 18 premises that had experienced an outbreak of fowl cholera within the past 2-8 months; samples were collected from 16 of these 18 premises within 2-8 weeks of outbreak notification and while the infected flock was still present. a total of 939 animals were trapped from an additional 18 premi ...19883382384
australian fraud inquiry under way. 19883386761
chemical synthesis of deuterated folate monoglutamate and in vivo assessment of urinary excretion of deuterated folates in man.the synthesis and in vivo application of stable-isotopically labeled folic acid was investigated to devise methods suitable for studies of folate metabolism in human subjects. glutamate-labeled tetradeutero-pteroylglutamic acid (d4-folic acid) was prepared by mixed anhydride coupling of n10-trifluoroacetylpteroic acid and dimethyl l-[3,3,4,4-2h4]glutamic acid, saponification in sodium deuteroxide, and chromatographic purification. retention of the isotopic label was verified by proton nmr and ma ...19883389521
identification of toxigenic pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation.toxigenic strains of pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. cells which were young and densely confluent were best suited to this assay. the incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. the agarose overlay method correctly ...19883390090
[the aging of pasteurella multocida aerosols]. 19883395181
comparison of conjunctival and corneal surface areas in rabbit and human.this comparative study shows the surface area ratio of conjunctiva to cornea to be two times larger in humans than in rabbits. this large heretofore unrecognized interspecies difference may affect the applicability of drug pharmacokinetic data obtained using rabbit models and should be taken into consideration in topical drug development and future comparative drug penetration studies between rabbit and man.19883409715
antigenically related iron-regulated outer membrane proteins produced by different somatic serotypes of pasteurella multocida.an 84-kilodalton outer membrane protein was expressed when pasteurella multocida, somatic serotype 3, was grown in brain-heart infusion broth containing the iron chelator dipyridyl but not in brain-heart infusion broth alone. antigenically related outer membrane proteins of various molecular masses were also expressed by p. multocida strains belonging to all of the other 15 somatic serotypes (somatic serotype 12 being the possible exception) as well as by isolates expressing somatic antigens rep ...19883410547
differentiation of toxigenic from nontoxigenic isolates of pasteurella multocida by enzyme-linked immunosorbent assay.an enzyme-linked immunosorbent assay (elisa) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of pasteurella multocida. the sandwich elisa is based on two different murine monoclonal antibodies with specificity for the p. multocida toxin. the elisa, which is now used as a routine test in denmark, has several advantages compared with previously described biological tests.19883410955
[effects of tortoise plastion and tortoise-shell on body weight, water intake, urination, plasma viscosity, etc., of hyperthyroidism-type yin-deficient rats]. 19883416388
chronosynergistic effects of lighting schedule-shift and cefodizime on plasmacytoma growth and host survival time.lighting regimen shifts can modify the effects of cefodizime, for the purpose of a chronoimmunomodulation. two experiments were carried out on male and female lou rats inoculated subcutaneously with plasmacytoma cells. some rats were kept on their original ld12:12 regimen, whereas others, after tumor implantation, were subjected every second day to 6-h shifts, instituted, in alternation, as advance or delay. daily treatment with cefodizime or placebo started when, overall, about 50% of the anima ...19883416672
optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes.four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ...19883417404
staphylococcus aureus endocarditis in a previously healthy adolescent.infectious endocarditis is occasionally a complication of staphylococcus aureus sepsis in previously well individuals with no heart disease or history of intravenous drug use. we report a case of a 16 year old who developed staphylococcal sepsis and endocarditis probably as the result of neglected paronychia of her toes. despite adequate antibiotic therapy, the infectious process destroyed her aortic valve, thereby producing aortic regurgitation complicated by cerebral embolism. aortic valve rep ...19883417509
in vitro and in vivo evaluation of c-20- and c-23-modified derivatives of tylosin against veterinary pathogens.three series of semi-synthetic derivatives of tylosin-related macrolides were evaluated for utility in veterinary medicine. 23-modified derivatives of 5-o-mycaminosyltylonolide (omt) possessed potent activity in vitro against species of pasteurella and mycoplasma. an experimental infection in chicks caused by pasteurella multocida was utilized to evaluate efficacy; several of these derivatives of omt effectively treated the infection when given subcutaneously, but none were effective after oral ...19883417566
initial processing of the insulin receptor precursor in vivo and in vitro.to evaluate the synthesis and initial processing of the insulin receptor precursor, we compared cell-free translation of rat liver poly(a)+ rna in a reticulocyte lysate system with metabolically labeled rat hepatoma (fao) cells. in in vitro translation assays, the primary l-[35s]cysteine-labeled products of rat liver mrna specifically immunoprecipitable with insulin receptor antiserum were two closely migrating polypeptides with a mr range of 160,000-164,000 (n = 7). this is similar to the size ...19883417632
infectious in vitro transcripts from cowpea chlorotic mottle virus cdna clones and exchange of individual rna components with brome mosaic virus.complete cdna copies of genomic rna1, rna2, and rna3 of cowpea chlorotic mottle virus (ccmv) were cloned 1 base downstream from a t7 rna polymerase promoter. the mixture of capped in vitro transcripts from all three clones produced normal ccmv infections in barley protoplasts and cowpea plants. by using transcripts from these clones and from a similar set of biologically active clones of the related brome mosaic virus (bmv), all possible single component exchanges between the bmv and ccmv tripar ...19883418781
ocular infections secondary to pasteurella multocida. 19883421301
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...19883421507
common genetic pools between human populations.a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...19883422217
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