Publications

survival of vibrio parahaemolyticus in two gastropod molluscs, clithon retropictus and nerita albicilla.		1988	3172602
isolation from a coastal fish of vibrio hollisae capable of producing a hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus.	a vibrio isolated from the intestine of a coastal fish was identified as vibrio hollisae by its biochemical characteristics. the isolate reacted with the gene probe for the thermostable direct hemolysin of vibrio parahaemolyticus. the hemolysin produced by the isolate from the fish had traits identical to those of the thermostable direct hemolysin-like hemolysin produced by a clinical strain of v. hollisae.	1988	3178217
temporal relationship of vibrio parahaemolyticus in patients and the environment.	we prospectively compared the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. inpatient and outpatient stool and wound specimens and water samples from 10 estuarine sites were cultured for v. parahaemolyticus over a period of 3 years. v. parahaemolyticus infections were detected in 13 patients (8 with gastroenteritis; 5 with wound infections), and all of the infections were found in outpatients in physicians' offices. ten of the infections were loc ...	1988	3183022
sodium butyrate increases glucose transporter expression in llc-pk1 cells.	the effect of sodium butyrate on the expression of the facilitated glucose transporter (gt) was investigated in the pig kidney cell line llc-pk1. when cells were treated with butyrate, gt mrna expression was remarkably enhanced with a maximal effect at 5 mm. levels of gt mrna were increased at 1 day after butyrate treatment and continued to increase for at least 4 days; however, acetate and propionate did not affect gt mrna levels significantly. the induction of gt mrna by butyrate was accompani ...	1988	3186708
hepatotoxicity of cyclophosphamide in man: pharmacokinetic analysis.	of 44 patients with neoplasia treated with cyclophosphamide (cpa), 19 (43%) had a transient elevation of serum levels of aminotransferases. in these patients, regardless of the combination chemotherapy regimen given, the incidence of liver dysfunction was 33%, when the total cpa dose was less than 400 mg/m2 (group a) and 73% with higher doses (group b). prior to the initiation of cpa treatment, pharmacokinetics of cpa were investigated in 15 patients. plasma aucs of cpa and phosphoramide mustard ...	1988	3187190
differential modulation of host cell and hiv gene expression by combinations of avarol and azt in vitro.	human immunodeficiency virus type 1 (hiv-1) gene expression is modulated by some virus-encoded proteins, possibly acting at multiple levels of control, which are also known to be involved in the regulation of gene expression in uninfected cells (transcriptional, post-transcriptional, nucleocytoplasmic transport, and translational control). two anti-hiv-1 drugs, avarol and 3'-azido-3'-deoxythymidine, which inhibit viral replication by differential mechanisms, were used to study the role of cytopl ...	1988	3190740
chicken liver basic fatty acid-binding protein (pi = 9.0). purification, crystallization and preliminary x-ray data.	chicken liver basic fatty acid-binding protein (pi = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. the final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. the crystals are either tetragonal, space group p4(2)2(1)2 with a = b = 60.2 a and c = 138.1 a or orthorhombic, space group p2(1)2(1)2(1) with a = 60.7 a, b = 40.1 a and c = 66.7 a. the second form appears to be more suitable f ...	1988	3191992
activities and properties of putrescine-biosynthetic enzymes in vibrio parahaemolyticus.	the biosynthetic pathways for putrescine (put) in vibrio parahaemolyticus were delineated by measuring activities of the enzymes which would be involved in its biosynthesis. experiments with labeled arginine and ornithine revealed that both of these amino acids were converted into put by intact cells. the activities of three enzymes, arginine decarboxylase (adc), ornithine decarboxylase (odc), and agmatine ureohydrolase (auh), were detected in cell extracts. adc and odc of v. parahaemolyticus we ...	1988	3193911
the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats.	the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ...	1988	3196152
[food poisoning caused by halophilic vibrio parahaemolyticus].		1988	3212540
coccidiocidal efficacy of a single treatment of toltrazuril in naturally infected lambs.		1988	3212906
[demonstration of new k-antigens in strains of vibrio parahaemolyticus isolated from patients with traveller's diarrhea].		1988	3216491
effect of aminooxyacetic acid on the release of preloaded [3h]gaba and radioactive metabolites from slices of developing mouse brain.	the release of [3h]gamma-aminobutyric acid (gaba) and its radioactive metabolites from slices of the cerebral cortex, cerebellum, striatum and brain stem of developing and adult mice was studied. the slices were incubated and superfused in the absence and presence of the gaba aminotransferase (gaba-t) inhibitor aminooxyacetic acid (aoaa). exposure to 100 microm aoaa totally inhibited gaba-t and all radioactivity released from slices was in authentic gaba. in studies on developing brain the 10-mi ...	1988	3216949
toxin profiles of vibrio cholerae non-o1 from environmental sources in calcutta, india.	a collection of vibrio cholerae non-o1 isolated from the aquatic environs of calcutta, a cholera-hyperendemic area, were examined for the production of cholera toxin (ct), shiga-like toxins (vero toxins), heat-stable enterotoxin, and hemolysins. two (0.5%) v. cholerae non-o1 isolates produced ct. the dna from both these isolates also hybridized with a dna probe containing sequences encoding the a subunit of ct. none of the strains produced shiga-like toxins or heat-stable enterotoxin. hemolytic ...	1988	3223774
chlamydial pneumonia of infancy in siblings.	we describe two brothers born 16 months apart who were hospitalized with culture-proved chlamydial pneumonia of infancy due to the same immunotype of chlamydia trachomatis. presumably the mother had either been chronically infected or had been reinfected. chlamydial pneumonia of infancy is a preventable disease if genital infection in pregnant women is diagnosed and treated. however, if pregnant women at high risk are not screened for c. trachomatis, multiple offspring may acquire the infection ...	1988	3227475
excision of cutaneous tumors in the horse using histologic guidance.	a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ...	1988	3227634
effects of endotoxemia on systemic plasma loss and hematocrit in rats.	endotoxemia in rats increases plasma extravasation but does not result in continuously rising hematocrit. these contradictory observations led us to design a study in anesthetized rats (c, control rats, n = 10; e, endotoxin rats, n = 10) in which we continuously measured in blood hematocrit (conductivity cell) and changes in concentration of 125i-hsa (human serum albumin) and 51cr-labeled red cell (51cr-rbc; multichannel analyzer) in an extracorporeal circuit. in two additional series of experim ...	1988	3231125
effect of ph, nacl, temperature and time of incubation on the probability of vibrio parahaemolyticus growth initiation in a model broth.		1988	3232624
consequences for swine farm income of depopulation as a disease control measure.		1988	3232640
individual free fatty acids and lactate uptake in the human heart during severe sepsis.	coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ...	1988	3232957
[kidney morphology in hemolytic-uremic syndrome].	morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ...	1988	3233069
[effectiveness of distal gene translation in polycistrons depends upon the arrangement of regulatory signals on a template].	the role of the translational terminator and initiator signals arrangement for two adjacent genes in polycistronic mrna has been studied. semisynthetic beta-galactosidase gene (lacz) of e. coli and fragment of phage m13 dna (with promoter pviii, gene ix, and part of gene viii) were used for constructing of the ix-viii-lacz artificial polycistronic operon. cloning of the constructs into pbr322 vector resulted in a number of plz381n plasmids differing by the mutual arrangement of gene viii transla ...	1988	3233097
partial characterization of a fraction from human follicular fluid that initiates the human sperm acrosome reaction in vitro.	a human follicular fluid (hff) fraction prepared by sephadex g-75 column chromatography has been previously shown by this laboratory to initiate the human sperm acrosome reaction (ar) in vitro. in the present report, the apparent molecular weight (mw) of this ar activity determined by a longer g-75 column than was used in the previous work was 50,000 +/- 5,106. the g-75 sephadex void volume fractions of some but not all hff samples were also found to contain some ar-initiating activity. the occa ...	1988	3235026
effect of ionizing radiation on macrophage stimulating property of vibrio parahaemolyticus lipopolysaccharide.		1988	3243588
the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat.	1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ...	1988	3256612
on the time-dependent diffusion of macromolecules through transient open junctions and their subendothelial spread. i. short-time model for cleft exit region.	in this two-part study we shall quantitatively study, using time-dependent models, the hypothesis that transient open junctions associated with widely scattered endothelial cells undergoing mitosis are the structural equivalent for the large pore pathway via which macromolecules the size of albumin or larger cross the vascular endothelium. in an earlier steady-state model [am. j. physiol. 248, h945-960 (1985)], the authors demonstrated that such an open-junction pathway could quantitatively acco ...	1988	3256708
[a lipoma of the cerebellopontile angle].	the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.	1988	3261397
[a lipoma of the cerebellopontile angle].	the case of a 38-year old man with a lipoma of the left cerebellopontine angle is presented. while the clinical signs of that tumour are unspecific, an exact diagnosis is possible by ct and mri. as in other reported cases only an incomplete removal of the tumours was made because of the adherence of the lipoma to the nerves of the cpa.	1988	3261397
interleukin 4 promotes the growth of tumor-infiltrating lymphocytes cytotoxic for human autologous melanoma.	addition of il-4 (1,000 u/ml) to either high or low concentrations of il-2 augmented tumor-infiltrating lymphocytes (til) growth from human melanoma. weekly restimulation with irradiated tumor cells in conjunction with il-4 allowed enhanced growth of til. with low-dose il-2 (10 u/ml) and il-4, expanded til had little cytolytic activity against daudi or allogeneic tumors. further, il-4 augmented the total lytic activity against autologous tumors in most cases. with high-dose il-2 (1,000 u/ml), il ...	1988	3264324
malaria infection in rats with stimulated splenic red pulp: the blood flow and protective effect in normal and transplanted splenic tissue.	after splenic autotransplantation both weight and blood flow of the regenerated splenic tissue are decreased. in addition, the protective function of the transplant is less compared to that of the normal spleen. in the present study, the red pulp of normal and transplanted splenic tissue was stimulated by injections of phenylhydrazine to increase the weight, the blood flow, and the protective function. after stimulation, the weight of the normal spleen increased to 900 +/- 70 mg (control 530 +/- ...	1988	3265528
megakaryocyte colony-stimulating factor and burst-promoting activity in lps-treated mouse spleen cell-conditioned medium.	in order to clarify the mechanism(s) of increased splenic hematopoiesis noted in lipopolysaccharide (lps)-injected mice, the effects of spleen cell-conditioned medium (spcm) on megakaryocyte colony (cfu-meg) formation and early erythroid (bfu-e) differentiation were investigated. after spleen cells from lps-injected mice were incubated for 3 days, the spcm was assayed for megakaryocyte colony-stimulating factor (meg-csf) in cfu-meg assay and for burst-promoting activity (bpa) and erythropoietin ...	1988	3265923
effect of recent antibacterial agents against bacteria causing diarrhoea.	the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...	1988	3266900
sensitivity of some marine bacteria, a moderate halophile, and escherichia coli to uncouplers at alkaline ph.	the inhibitory effects of uncouplers on amino acid transport into three marine bacteria, vibrio alginolyticus 118, vibrio parahaemolyticus 113, and alteromonas haloplanktis 214, into a moderate halophile, vibrio costicola nrc 37001, and into escherichia coli k-12 were found to vary depending upon the uncoupler tested, its concentration, and the ph. higher concentrations of all of the uncouplers were required to inhibit transport at ph 8.5 than at ph 7.0. the protonophore carbonyl cyanide m-chlor ...	1988	3045092
current perspectives on the epidemiology and pathogenesis of clinically significant vibrio spp.	recent taxonomic advances have now implicated several different vibrio species as human pathogens. while the most common clinical presentation of vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. detection of vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to vi ...	1988	3058295
[absence of vibrio parahaemolyticus in raw fish].		1988	3064232
insulin controlled-release microcapsules to prolong the hypoglycemic effect in diabetic rats.	a solvent evaporation process was employed for preparing insulin microcapsules by using biodegradable polymers [polylactic acid (pla), hp-55] and non-biodegradable polymers [ethylcellulose (ec), ethylenevinyl acetate (eva)]. the release behavior of insulin microcapsules in ph 7.4 phosphate buffer solution was undertaken by a continuous flow column method. seven types of insulin microcapsules were respectively injected into the flanks of fasting-diabetic sd rats induced by streptozotocin. the glu ...	1988	3064829
the role of the ras oncogene in the formation of tumours.	a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ...	1988	3075615
the role of the ras oncogene in the formation of tumours.	a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ...	1988	3075615
purification and characterization of a hemolysin produced by a clinical isolate of kanagawa phenomenon-negative vibrio parahaemolyticus and related to the thermostable direct hemolysin.	a clinical isolate (strain 4037) of kanagawa phenomenon-negative vibrio parahaemolyticus was studied. although the strain was judged to be kanagawa phenomenon-negative by various conventional tests, it produced a new hemolysin (named vp-trh, for thermostable direct hemolysin [vp-tdh]-related hemolysin) that was related to the vp-tdh produced by ordinary kanagawa phenomenon-positive v. parahaemolyticus. vp-trh was purified by ammonium sulfate fractionation and successive column chromatographies o ...	1988	3126151
decreased locomotor activity produced by repeated, but not single, administration of murine-recombinant interferon-gamma in mice.	a single treatment with murine-recombinant interferon-gamma (murifn-gamma; 30 micrograms/mouse), whether evaluated immediately after, or four hrs after, intraperitoneal injection, does not alter open field activity levels. on the other hand, repeated murifn-gamma administration (30 micrograms/mouse/day for 5 days) results in decreased spontaneous locomotor activity and an increase in body weight.	1988	3126371
the relationships between oligomeric structure and function of band 3 protein from human erythrocyte membranes: present knowledge and suggestions for further experiments.		1988	2976450
mononuclear cell infiltration in central portions of human astrocytomas.	the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...	1988	2830373
mononuclear cell infiltration in central portions of human astrocytomas.	the mononuclear cell infiltration in the central portion of human low- and high-grade astrocytomas has been examined using monoclonal antibodies to cell surface differentiation antigens. there was no significant difference between the number of t cells, b cells, or macrophages. the okt8+ (suppressor/cytotoxic) t cell population was the major lymphocyte subset in the infiltrates. there was, however, indirect evidence for a proportion of the total okt8+ cells being natural killer cells. overall, t ...	1988	2830373
prevalence of enteric pathogens in homosexual men with and without acquired immunodeficiency syndrome.	we studied 388 homosexual or bisexual men from the baltimore-washington area to define the spectrum of enteric pathogen carriage in a population at high risk for "gay bowel syndrome" in association with human immunodeficiency virus infection. seventy-seven patients with acquired immunodeficiency syndrome, 68 gay men with symptoms of acute diarrhea or proctitis, and 243 gay men without gastrointestinal symptoms and participating in a natural history study of human immunodeficiency virus infection ...	1988	2831107
characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58.	overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ...	1988	2832379
fibronectin is a component of the sodium dodecyl sulfate-insoluble transglutaminase substrate.	liver plasma membranes contain a morphologically distinct protein complex which serves as a substrate for the plasma membrane-associated transglutaminase. the complex, which appears as a two-dimensional sheet, is insoluble in sodium dodecyl sulfate and reducing agents and has been named sits for sodium dodecyl sulfate-insoluble transglutaminase substrate (tyrrell, d. j., sale, w. s., and slife, c. w. (1988) j. biol. chem. 263, 1946-1951). polyclonal antibodies raised against sits were used to pr ...	1988	2897368
effect of human growth hormone-releasing factor (1-29)nh2 on growth hormone release and milk production in dairy cows.	twelve cows (209 d in lactation, 642 kg bw) were used in an experiment conducted over four 10-d periods (one preinjection, one injection, and two postinjection). gelatine (n = 6) or 10 mg of growth hormone-releasing factor in gelatine (n = 6) was injected subcutaneously at 1000 h every day on d 11 to 20. data were averaged for the last 5 d of each period. during the injection period, milk, fat, and protein yields increased by 3 kg.d-1 (14.3%), .14 kg.d-1 (16.7%), and .12 kg.d-1 (15.4%), respecti ...	1988	2897385
the tissue distribution of the 3 alpha-fucosyl-n-acetyl lactosamine determinant recognized by the cd15 monoclonal antibodies cmrf-7 and 27.	two monoclonal antibodies, cmrf-7 and 27, which react with cells of the granulocytic series, were obtained from hybridomas cloned from separate fusions. biochemical studies indicate that both antibodies are of the cd15 group and react with the antigenic determinant 3 alpha-fucosyl-n-acetyl lactosamine (hapten x) expressed on some glycolipids and several different granulocyte glycoproteins with a wide range of molecular weights. the antigen was found on some promyelocytes and more differentiated ...	1988	2897656
gastric secretion and gastrin under progressive doses of somatostatin-14 and -28 administered intraperitoneally to the rat.	the actions of progressive doses of intraperitoneally (ip) administered somatostatin-14 (ss-14) and -28 (ss-28) on gastric secretion (acid, pepsin) and mucosal blood flow (mbf) were studied in conscious gastric fistula rats both under basal conditions and under additional administration of pentagastrin. also, somatostatin-like immunoreactivity was measured in aortal blood in all groups as well as aortal gastrin levels under basal conditions. ip infusion of equimolar doses of ss-14 and ss-28 resu ...	1988	2897678
importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function.		1988	2900297
responses of hippocampal pyramidal cells to putative serotonin 5-ht1a and 5-ht1b agonists: a comparative study with dorsal raphe neurons.	in low cerveau isolé transected rats, the effects of microiontophoretic application of putative serotonin 5-ht1a and 5-ht1b agonists on the spontaneous firing rate of ca1 pyramidal cells were compared to those of 5-ht. in contrast to the large current-dependent suppression of unit activity observed with 5-ht, the 5-ht1a compounds, ipsapirone, 8-oh-dpat (8-hydroxy-2-(di-n-propylamino)-tetralin) and ly 165163 (p-aminophenylethyl-m-trifluoromethylphenylpiperazine) and the 5-ht1b compounds, mcpp (m- ...	1988	2901680
evidence of functional alpha 2-adrenergic receptors in adult-rat adipocytes by using the agonist uk 14304.	the aim of this study was to re-assess whether alpha 2-adrenergic receptors were present in rat adipocytes, by using uk 14304, a new and very selective alpha 2-agonist. the following observations demonstrate the presence of functional alpha 2-adrenoceptors in rat adipocytes. (1) adipocyte lipolysis was dose-dependently inhibited by uk 14304 (maximal effect 80% at 1 microm-uk 14304, 45% at 10 microm-uk 14304, under basal or theophylline-stimulated conditions respectively). (2) uk 14304 bound spec ...	1988	2901830
effect of oversulphated chondroitin and dermatan sulphate upon thrombin and factor xa inactivation by antithrombin iii or heparin cofactor ii.	the kinetics of inhibition of human thrombin and factor xa by antithrombin iii or heparin cofactor ii were examined under pseudo-first-order conditions as a function of the concentration of naturally occurring oversulphated chondroitin and dermatan sulphates. the sulphated glycosaminoglycans (gags) studied were chondroitin sulphate d (csd) (glca-2-so4-galnac-6-so4), chondroitin sulphate k (csk) (glca-3-so4-galnac-4-so4), chondroitin sulphate h (csh) (ida-galnac-4,6-diso4) and polysulphated derma ...	1988	2902851
production of pili on vibrio parahaemolyticus.	electron microscopic examination showed that all strains of vibrio parahaemolyticus examined had pili on their surface when the organism was grown on marine agar at 28 degrees c for 6-12 h. the pili were morphologically stable on heat treatment at 60 degrees c for 10 min, but both the lateral and polar flagella possessed by this organism were labile. no immunological cross-reactivity between pili of enterotoxigenic escherichia coli and vibrio cholerae non-01 and those of v. parahaemolyticus was ...	1988	2905206
[purification and characterization of vibrio parahaemolyticus pili isolated from a patient with diarrhea: comparative studies with vibrio cholerae o1 pili].		1988	2907917
human cytomegalovirus-associated dnase and the specific immune response in different clinical conditions.		1988	2837245
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.	in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...	1988	2838023
egg shell quality and cholecalciferol metabolism in aged laying hens.	calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ...	1988	2841436
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
modulation of phosphoinositide hydrolysis by naf and aluminum in rat cortical slices.	naf stimulated phosphoinositide hydrolysis in rat cortical slices. the production of [3h]inositol monophosphate was rapid for the first 15 min of incubation with naf, followed by a plateau. the major product detected was [3h]inositol monophosphate, although significant amounts of [3h]inositol bisphosphate and [3h]inositol trisphosphate were also produced. the stimulation of [3h]inositol monophosphate production by naf was concentration dependent between 2 and 20 mm naf. addition of 10 or 100 mic ...	1988	2846782
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.	sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...	1988	2847397
the potassium channel opening action of pinacidil; studies using biochemical, ion flux and microelectrode techniques.	in rat aorta and rat portal vein, (-)- and (+)-pinacidil each produced a concentration-dependent inhibition of tension development. although the (-) isomer was the more potent, concentration effect curves for each isomer were steep with similar slopes. in rat portal vein, tetraethylammonium and procaine antagonised the relaxant effect of (+/-)-pinacidil, whereas 3,4-diamino-pyridine was without effect. intracellular microelectrode recording in rat portal vein showed that low concentrations of (+ ...	1988	2848206
factors influencing the severity of escherichia coli and avian adenovirus group ii infections in chickens.	chickens from lines selectively bred for either a high (hh) or low (ll) antibody response to sheep erythrocytes were challenged intravenously with avian adenovirus group ii (aa). spleen size was determined 6 days later. in some experiments the responses of chickens to aa and escherichia coli infections were compared. the level of corticosterone in the feed (15 mg/kg) which resulted in the lowest incidence of pericarditis in response to e. coli resulted in the greatest incidence of large spleens ...	1988	2849405
plasma from uninephrectomized rats stimulates production of inositol trisphosphates and inositol tetrakisphosphate in renal cortical slices.	metabolism of inositol phosphates in renal cortical slices was investigated in vitro after addition of plasma from uninephrectomized or sham-operated rats. plasma from uninephrectomized rats stimulated production of insp3 (inositol trisphosphate) when obtained within the first 3 h after uninephrectomy. with different amounts of added plasma a graded response of insp3 production was obtained. this effect could be prevented by 0.1 microm-tpa (12-o-tetradecanoylphorbol 13-acetate). when analysis of ...	1988	2849423
kininase ii from human seminal plasma. isolation and properties.	a rapid and highly efficient procedure for purification of kininase ii from human seminal plasma is described. after ultra centrifugation, the enzyme was purified by gel filtration on sepharose 6b cl and ion exchange chromatography followed by affinity chromatography of edta-inhibited enzyme on bradykinin-sepharose. the enzyme was specifically inhibited by captopril and bpp9a but not by phosphoramidon. page in the presence of sodium dodecyl sulfate under reducing conditions resulted in two major ...	1988	2849459
suppression of sv40-promoted gene expression by differentiation of preadipose cells.	when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...	1988	2849579
an immunohistochemical evaluation of c-erbb-2 expression in human breast carcinoma.	the c-erbb-2 gene codes for a putative transmembrane protein, similar in structure to the epidermal growth factor (egf) receptor. amplification of the gene has been described in a variety of human adenocarcinomas and is particularly well documented in breast carcinoma. it has been suggested that amplification is indicative of poor prognosis and, as such, is comparable with lymph node status as a predictor of clinical outcome. this study examines the suggestion indirectly by an immunohistochemica ...	1988	2849974
thermostable direct hemolysin of vibrio parahaemolyticus.		1988	2852761
establishment and characterization of an epstein-barr virus negative b-cell lymphoma cell line and successful heterotransplantation.	an ebv-negative b-cell lymphoma cell line designated as hbl-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. surface marker studies revealed that hbl-1 cell possessed the same phenotypes of a mature b-cell as the original lymphoma cell from pleural effusion. hbl-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. chromosome analysis revealed that hbl-1 cells showed chromos ...	1988	2854303
heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells.	cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ...	1988	2446745
hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987.		1988	3136308
vibrio parahemolyticus-associated diarrhea in spain.		1988	3137059
development of a highly sensitive bead-elisa to detect bacterial protein toxins.	a highly sensitive sandwich enzyme-linked immunosorbent assay to detect bacterial toxins was developed. fab' of anti-toxin igg was conjugated with horseradish peroxidase by the maleimide method and tetramethylbenzidine was used as substrate. as the solid phase, a 6.5 mm diameter polystyrene bead was used and this was coated with the anti-toxin igg. the entire assay could be completed within 3.5 hr. the sensitivity of this bead-elisa was found to be quite high with various bacterial toxins: less ...	1988	3143897
poor lifestyle modification among intravenous drug users assisted at a sexually transmitted disease clinic for hiv infection diagnosis, madrid, 1987.		1988	3146273
prevention of respiratory distress syndrome.	rds continues to be a major problem for premature infants despite a better understanding of its pathophysiology and of ways to try to prevent it. to date, prenatal administration of glucocorticoids has been the most widely used method of accelerating fetal lung development. however, several limitations of this therapy have prompted the search for alternative approaches. most efforts have focused on the potential use of combined hormonal therapy with glucocorticoids and either thyroid hormones or ...	1988	3146813
isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats.	lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ...	1988	3147719
[effects of intracerebroventricularly injected cholecystokinin octapeptide on the electric activities of pain-reacted neurons in nucleus parafascicularis of thalamus in rats].		1988	3151154
serum albumin adducts in the molecular epidemiology of aflatoxin carcinogenesis: correlation with aflatoxin b1 intake and urinary excretion of aflatoxin m1.	aflatoxin-serum albumin adducts in the blood of 42 residents of guangxi province, people's republic of china, were determined and compared with intake of aflatoxin b1 (afb1) and excretion of aflatoxin m1 (afm1) in urine. blood specimens were obtained during the same period that urine was collected and that diet was sampled. serum albumin was isolated from blood by affinity chromatography on reactive blue 2-sepharose and subjected to enzymatic proteolysis using pronase. immunoreactive products we ...	1988	3133131
prostacyclin biosynthesis and phospholipase activity in hypoxic rat myocardium.	phospholipid metabolism was studied in rat myocardial slices that were incubated under normoxia or hypoxia for up to 24 hours. phospholipid degradation was prominent in hypoxic myocardium, particularly phosphatidylcholine, which markedly decreased after 24 hours of hypoxia. in contrast, lysophosphatidylcholine increased. the mechanism of phospholipid degradation in hypoxic myocardium was studied. the highest activity for phospholipase a2 among subcellular fractions was found in microsomal fracti ...	1988	3133132
bone mineral metabolism in experimental diabetes mellitus: osteocalcin as a measure of bone remodeling.	the etiology of diabetic osteopenia has not been established. the value of serum osteocalcin (bgp) as a marker of the bone abnormalities and the possible role of the polyol pathway in diabetic osteopenia were investigated. three groups of rats were studied over 7 weeks: group d (n = 12), rats with streptozotocin (55 mg/kg)-induced diabetes given saline by gavage; group ds (n = 12), rats with streptozotocin-induced diabetes given the aldose reductase inhibitor sorbinil (25 mg/kg) daily by gavage; ...	1988	3133194
effects of acetyl strophanthidin on duration of atrial fibrillation in the neurally-intact and blockaded dog.	although the inotropic and dromotropic effects of cardiac glycosides in atrial fibrillation (af) are well recognized, their action on af itself is not clear. accordingly, to determine whether cardiac glycosides prolong af, the duration of electrically induced af, atrioventricular conduction, and left ventricular function were assessed for 30 minutes before and for 30 minutes following intravenous administration of acetyl strophanthidin (as), 20 micrograms/kg, in neurally intact, beta-blocked, an ...	1988	3154634
dose-dependent effects of oral and intravenous glucose on insulin secretion and clearance in normal humans.	insulin secretion and clearance were studied in 2 groups of 7 normal subjects who each received 25, 50, and 100 g of glucose either orally or intravenously (iv) on separate occasions. insulin secretion rates were calculated during a 1-h base line and for 5 h after glucose administration from a two-compartmental analysis of peripheral c-peptide concentrations using individual kinetic parameters derived after iv bolus injections of biosynthetic human c-peptide. incremental glucose areas after oral ...	1988	3279811
evaluation of four methods for enumeration of vibrio parahaemolyticus.	two membrane filter (mf) and two most-probable-number methods for enumerating vibrio parahaemolyticus were compared. the mf methods used elevated-temperature incubations (41 and 42 degrees c) and were more specific than the most-probable-number methods (conducted at 35 degrees c). the mf method with a hydrophobic grid and a repair step was most effective.	1988	3281584
[immunodiagnosis of aids using preparations of the recombinant antigen of the surface protein of the htlv-iii virus].	a technological scheme has been developed for purification of recombinant antigen of surface protein (asp) of the causative virus of aids from escherichia coli cells carrying plasmid pl2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda n protein (59 amino acid residues) and a fragment of sp (env) of hiv virus (569 a.r.). purification of asp is based on two separation principles: fractionation of polypeptides of bacterial lysates by preparative isoelectrofocusing i ...	1988	3285593
an experimental study of susceptibility to infection after hemorrhagic shock.	hemorrhagic shock has been associated with an increased risk of infection after injury. the immediate and long term effects of hemorrhagic shock without tissue injury on the susceptibility of an animal to infection and the efficacy of antibiotic prophylaxis to prevent infection in this setting were examined. sprague-dawley rats were subjected to hemorrhagic shock (ld15) by bleeding to a mean arterial pressure of 45 millimeters of mercury for 45 minutes and were resuscitated with shed blood and n ...	1989	2911791
renal metabolism of the oxidized form of ascorbic acid (dehydro-l-ascorbic acid).	we evaluated whether specific transport and metabolic properties exist in rat and guinea pig kidney for handling the immediate oxidative product of ascorbic acid, dehydro-l-ascorbic acid. isolated tubules were used to measure uptake of 10 microm [14c]-dehydro-l-ascorbic acid over an 8-min incubation period. uptake did not show dependence on the bathing media electrolyte composition but was inhibited to some extent by glucose. in tubules of both animal species the majority of 14c label present in ...	1989	2912166
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
iron regulation of swarmer cell differentiation of vibrio parahaemolyticus.	vibrio parahaemolyticus has two distinct cell types, the swimmer cell and the swarmer cell, adapted for locomotion in different circumstances. the swimmer cell, produced when the bacterium is grown in liquid media, is a short rod with a single sheathed polar flagellum. the swarmer cell, produced when v. parahaemolyticus is grown on solidified media, is greatly elongated and synthesizes, in addition to the polar flagellum, numerous unsheathed lateral flagella which are responsible for translocati ...	1989	2914871
saturable binding sites mediate the entry of african swine fever virus into vero cells.	binding experiments of 3h-labeled african swine fever virus to susceptible vero cells have shown the presence of saturable binding sites for african swine fever virus on the plasma membrane. the scatchard analysis of the binding data at equilibrium indicates the existence of about 10(4) cellular receptor sites per cell with a dissociation constant (kd) of 70 pm. virus entry into vero cells is mediated by a saturable component, since tritiated african swine fever virus saturable binding and uptak ...	1989	2916331
britain launches genome program.		1989	2928800
[phylogenetic analysis of genes of the influenza virus. relationship between adaptability and neutrality].	detailed phylogenetic analysis of the gene family of hemagglutinin h3 of influenza a-type virus was fulfilled, taking into account the domain structure of protein and positions of antigen determinants. the densities of distribution of fixed synonimic replacements between domains ha1 and ha2 were shown to be actually equal (rho (ha1) = rho (ha2], and those of nonsynonimic ones to be unequal: their ratios were rho (ha1): rho (ha2) = 2.8 for nonepidemic branches, and rho (ha1): rho (ha2) = 7.7 for ...	1989	2583491
evidence of immunologic cross-reactivity between hemolysins of vibrio hollisae and vibrio parahaemolyticus demonstrated by monoclonal antibodies.		1989	2584761
comparative amino acid sequence analysis of hemolysins produced by vibrio hollisae and vibrio parahaemolyticus.	vibrio hollisae produces a hemolysin (vh-rtdh) that is related to the thermostable direct hemolysin of vibrio parahaemolyticus (vp-tdh). although both hemolysins are essentially similar biologically and immunologically, they differ markedly in heat stability; vp-tdh is heat stable, whereas vh-rtdh is heat labile. to elucidate the relationships between their characteristics and molecular structures, we analyzed the amino acid sequence of vh-rtdh and compared it with that of vp-tdh. vh-rtdh consis ...	1989	2592352
urease-positive, kanagawa-negative vibrio parahaemolyticus from patients and the environment in the pacific northwest.	we previously reported the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. the present studies compare the biochemical characteristics, kanagawa hemolysin reactions, and plasmid profiles of 13 patient and 221 environmental isolates of the organism. classical biochemical testing of the isolates revealed similar reactions for the clinical and environmental strains, and analysis in agarose gels revealed that 13 to 15% of the isolates had plasmids. the ...	1989	2592543
emergence of a restricted bioserovar of vibrio parahaemolyticus as the predominant cause of vibrio-associated gastroenteritis on the west coast of the united states and mexico.	examination of 45 human fecal isolates of vibrio parahaemolyticus revealed the emergence of an unusual bioserovar (o4:k12, urease positive) associated with cases of gastroenteritis which appear to be domestically acquired on the west coast of the united states and mexico.	1989	2592553
advances in oculoplastic surgery.	ophthalmic plastic surgery, a sub-specialty within ophthalmology, encompasses diagnosis and treatment of diseases and abnormalities of the eyelids, orbit, and lacrimal systems. eyelid carcinoma is a more common entity as well as blepharoptosis seen in children and adults. it can usually be corrected by surgery involving the levator palpebrae superioris or a tarsofrontalis suspension using synthetic material or human tissue. retraction of the upper eyelid, the opposite of blepharoptosis, may be s ...	1989	2592933
exotic diarrhoeal problems and poliomyelitis.	exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death.	1989	2594659
identification of n1-acetylnorspermidine in vibrio parahaemolyticus and an enzyme activity responsible for its formation.	n1-acetylnorspermidine [ch3conh(ch2)3 nh(ch2)3nh3] was identified in vibrio parahaemolyticus, which contains norspermidine as a major polyamine. this is the first example for the natural occurrence of monoacetylated unusual polyamine. the n1-acetylnorspermidine content was the highest 4 h after inoculation. incubation of norspermidine and acetyl coa with a cell extract from v. parahaemolyticus produced n1-acetylnorspermidine. a remarkable increase in specific activity of the acetyltransferase wa ...	1989	2599358
a mutant hemolysin with lower biological activity produced by a mutant vibrio parahaemolyticus.	a mutant toxin (m-tdh) of thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus was isolated from the culture of a strain of this organism mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. although the m-tdh had a molecular structure similar to the native vp-tdh, the m-tdh retained only about 7% residual hemolytic activity of the native toxin. furthermore, other biological activities of m-tdh, such as lethality in mice and enterotoxicity in rabbit ileal loops, were also weakened ...	1989	2599363
symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984.	the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ...	1989	2609210