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occurrence of squalene and sterols in cellulomonas dehydrogenans (arnaudi 1942) comb. nov. hester 1971.the neutral lipid fraction of the photochromogenic, coryneform bacterium cellulomonas dehydrogenans (arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. the compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. de novo sterol biosynthetic ability was shown from incorporation of 14c from d-[u-14c]glucose into squalene and the sterol fraction. the squalene concentration ...1978101527
squirrel monkey temporary threshold shift from 48-h exposures to low-frequency noise.five squirrel monkeys were exposed for 1, 2, 4, 8, 16, 24, and 48 h to a 375--750-hz band noise at an overall spl of 95 db. the tts4.5 growth pattern for the 750-hz test frequency was biphasic and did not reach an asymptote after 48 h of exposure. for all exposures, the mean thresholds of the five monkeys returned to within 5 db of the preexposure mean 20 h after exposure. recovery curves from all exposures at the 750-hz test frequency appeared biphasic. increasing spl from 95 to 105 db increase ...1978101572
crystallographic data on a complete kappa-type human bence-jones protein. 1978101675
studies on virus-inhibiting substances of bacterial origin. iv. antiviral substances extractable from streptococcus faecalis: its in vivo effect. 1978101714
[the effect of prolonged irradiation and of certain radioprotective agents on glycosaminoglycans in the rat]. 1978101997
temporal relationship of translation and glycosylation of immunoglobulin heavy and light chains.the initial glycosylation of mpc 11 gamma 2b heavy chains occurs quantitatively in vivo when the nascent heavy chains reach a size of approximately 38 000 daltons. nonglycosylated, completed mpc 11 heavy chains cannot be glycosylated in these cells. other classes of mouse heavy chains (i.e., mu, alpha, and gamma 1) also appear to be glycosylated as nascent chains; nonglycosylated, completed heavy chains cannot be glycosylated by the cell in any of these cases. in contrast, variant mpc 11 cells s ...1978103571
single-unit activity in the auditory cortex of monkeys selectively attending left vs. right ear stimuli.single-unit responses were recorded from the auditory cortex of rhesus monkeys that were performing an auditory selective attention task. acoustic stimuli were presented randomly to either the left or right ear through headphones. in a given block of trials one ear was selected as the ear to be attended, and was indicated by lighting either a left or right response key. the animal's task was to press the lighted key whenever stimuli were presented to the attended ear, but to make no response to ...1978103597
organization of the sympathetic innervation in liver tissue from monkey and man.the sympathetic innervation of the liver of monkey and man has been investigated in a combined fluorescence histochemical, chemical and electron microscopical study. by means of the falck-hillarp fluorescence method a dense network of monoamine-containing nerve fibers was visualized in liver tissue of monkey and man. the nerve fibers ran in close contact to both hepatocytes and blood vessels. chemical quantitations showed high concentrations of noradrenaline in both human and monkey liver. micro ...1978103622
oligosaccharides and polysaccharides specifically utilizable by bifidobacteria. 1978103638
[effect of lymphokines produced in a grafted system on production of prostaglandins by macrophages].mice peritoneal macrophages cultured in vitro release a prostaglandin-like activity as evaluated by a bio-assay using rat stomach fundus. the prostaglandin release is greatly increased when macrophages are incubated with the supernatant of mixed lymphocyte cultures between recipient and donor of a skin allograft. this phenomenon was found in all strains of mice tested except c3h/hej mice, a strain already known for its defective responsiveness to bacterial lipopolysaccharide (lps).1978103646
occurrence and distribution of western equine encephalomyelitis in florida.research and surveillance programs relating to the occurrence and distribution of western equine encephalomyelitis virus in florida, conducted between 1955 and 1976, suggest that the virus is (1) an endemic arbordae, (2) transmitted in a continuous cycle throughout the year by culiseta melanura mosquitoes, and (3) restricted to fresh water swamps and waterways in central, north, and northwest florida.197823372
aerobic glycolysis in bone: lactic acid production by rat calvaria cells in culture.considerable data have been accumulated on aerobic glycolysis of intact bone preparations. to test whether aerobic glycolysis is a feature of the bone cells themselves or of the localized conditions within an intact bone, cells isolated from rat calvaria were cultured and the effect of several factors on lactate production was determined. these cells drastically decreased lactate production when the ph in the culture medium was lowered, changing from 100 to 20 percent for a ph shift from 7.4 to ...197823680
dipeptidylaminopeptidase and carboxypeptidase activities of the brush border of rabbit small intestine. 197830675
the ph-dependence of the non-specific esterase activity of carboxypeptidase a.the hydrolysis of the following 6 esters by bovine pancreatic carboxypeptidase a (peptidyl-l-amino-acid hydrolase, ec 3.4.12.2) has been investigated over the range ph 5--10 at 25 degrees c, ionic strength 0.2: ch3co2chrco2h (r = c6h5 (ester 3), c6h5ch2 (ester 4), 4-no2c6h4co2chrco2h (r = c6h5 (ester 5), c6h5ch2 (ester 6), ch3(ch2)2 (ester 7)), ch3ch2co2ch(ch2c6h5)-co2h (ester 9). for each ester the ph dependence of kcat/km indicates that substrate binding is controlled by an acid of pkeh = 9.2 ...197831187
[study of the dynamics of acid formation in the stomachs of dogs under the influence of thyroidin by the method of intragastric ph-graphy].intragastral ph-graphy was applied to the study of the dynamics of acid production in the stomach of dogs with fistulae of the fundal portion of the stomach out of and during digestion, and also before and after the thyroidin prescription. biphasic character of the changes in acid production in the stomach under the effect of the hormonal preparation was revealed.197831210
proceedings of the fifteenth congress of the european dialysis and transplant association, istanbul, turkey, 1978. 197833380
[levels of testosterone and dihydrotestosterone in guinea pig sex canals during embryonic life].at the undifferentiated stage, testosterone and dihydrotestosterone levels were comparable in sexual ducts of both sexes. an hormonal sexual dimorphism appeared at the time of differentiation: while testosterone rapidly decreased in the female ducts, it considerably increased in those of the male. testosterone rather than dht seems to be responsible for the wolffian duct stabilization in the male foetus.197895894
molybdenum independence of nitrogenase component synthesis in the non-heterocystous cyanobacterium plectonema.the cyanobacterium plectonema boryanum (iu 594-utex 594) fixes n2 only in the absence of combined n and of o2. we induced nitrogenase by transfer to anaerobic n-free medium and studied the effect of mo starvation on nitrogenase activity and synthesis. activity was first detected within 3 h after transfer by the acetylene reduction assay in controls, increasing for at least 25 h. cells grown on nitrate and mo and then transferred to n-free, mo-free medium produced 8% of the control nitrogenase ac ...197896092
direct administration of methotrexate into the central nervous system of primates. part 1: distribution and degradation of methotrexate in nervous and systemic tissue after intraventricular injection.levels of methotrexate (mtx) measured by both 3h radioactivity and dihydrofolate reductase assays were determined in cerebrospinal fluid (csf), plasma, urine, and both neural and non-neural tissues at varying times after a single intraventricular injection into cynomolgus monkeys (macaca fascicularis). clearance of the mtx from csf was rapid after injection. a relatively constant level of 3hmtx was reached in plasma 2 1/2 hours after injection, and about 30% of the 3hmtx dose was excreted in the ...197896228
tissue tropism of different trypanosoma cruzi strains.a systematic study of the distribution of intracellular parasites in the organs and tissues was performed in groups of mice inoculated with 4 different trypanosoma cruzi strains. an extremely high parasitism of spleen, liver, and bone marrow was observed in mice inoculated with y and berenice strains; with cl strain, however, parasites were almost absent in those organs. bloodstream forms apparently present differences which facilitate or prevent their uptake by macrophages from the mononuclear ...197896243
variant of stiff-man syndrome provoked by alcohol: case report. 197896364
chromosomal locations of mouse immunoglobulin genes.the chromosomal locations of the structural genes coding for the constant portions of mouse heavy (h) and light chain immunoglobulins were studied by molecular hybridization techniques. complementary dna probes containing the constant-region sequences of kappa and lambdai light chain and alpha, gamma2b, and mu heavy chain mrnas were annealed to a large excess of dna from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. the lines were scored as pos ...197896442
effects of ethanol on pregnant rats and their offspring.pregnant rats were intubated with either 1.0 or 2.0 g/kg of ethanol daily throughout gestation. pair-fed vehicle-treated, and nontreated rats fed ad libitum, served as control groups for ethanol-treated animals. ethanol treatment reduced food and water consumption and attenuated the gain in body weight of pregnant animals relative to nontreated animals fed ad libitum. litter size, litter weight, and the mean weight per pup were reduced in both the ethanol-treated and pair-fed control groups. the ...197896469
[oesophageal diverticulum in a pony (author's transl)].a case of oesophageal diverticulum in a pony is reported. the diagnosis was established by clinical and x-ray examination (following administration of a contrast agent) and verified by post-mortem studies. the cause and pathogenesis are discussed.197896547
haplotype designations of various strains of guinea pigs. 197896556
[biological properties and mechanisms of development of bacteria resistant to fusidin, erythromycin and chloramphenicol]. 197896638
phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver.rat liver phospholipids were radioactively labeled in vivo before purification of udp-glucuronyltransferase to homogeneity. the pure enzyme contained very little phospholipid (approx. 0.7 mol of phospholipid/mol of protein). the solubilization detergent lubrol 12a9 appeared to act as a phospholipid substitute, capable of supporting udp-glucuronyltransferase activity. phospholipase c did not inhibit the pure enzyme activity and pure udp-glucuronyltransferase was stimulated by 40--100% by the addi ...197896816
5-thio-d-glucose is an acceptor for udp-galactose : d-glucose 1-galactosyltransferase. 197896819
2-aminoethylphosphonic acid as an indicator of tetrahymena pyriformis w growth in protein-quality evaluation assay.1. the concentration of 2-aminoethylphosphonic acid (aep) in 96 h cultures of tetrahymena pyriformis w was studied in order to apply it as an indicator in the assay of the relative nutritive value (rnv; protozoa population with test protein:protozoa population with whole-egg powder) of protein. foodstuffs and food mixtures of different protein contents and qualities were used as test samples. 2. rnv values based on aep determination (rnvaep) were compared with corresponding values calculated fro ...197896851
phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans.stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ...197896857
comparison of effects of normal rabbit sera and anti-cock sperm sera on rabbit sperm, including comparison of effects on the sex ratio. 197896880
[radioimmunological aspects of neuropeptides].immunological aspects of neurosecretory peptides are relevant to antibody generation, labelling of peptides for radioimmunoassays and in particular the metabolism of neuropeptides. antibodies were generally produced by adsorption or polymerization (using different approaches and various coupling agents) of peptides with intrinsic antigenic molecules preceding their administration to recepient animals (rats, guinea pigs, rabbits and sheep) with adjuvants. antibodies were also obtained by injectin ...197896897
[formation of cutaneous nerve trunks in the chick. ultrastructural and quantitative analysis].in chick skin structuration of nervous trunks takes place at 14-15 days of incubation, at the time of formation of the adult pattern of cutaneous innervation. at this same stage, myelogenesis begins, but develops mainly after hatching: only 4% of axons are myelinated at hatching, 40% in 6-week old chickens and 60% in adults. this "critical" stage (14-15 days of incubation) apparently corresponds to the stabilization of cutaneous nerve supply.197896985
[nature of the mutations that determine the ability of bacillus subtilis a-50 to sporulate at high glucose concentrations in the medium].the ability of bacillus subtilis a-50 to sporulate in the medium containing high glucose concentrations is caused by at least two mutation types: pts mutations and cat (or tgl) mutations, both of them affecting differently the level of alkaline proteinase synthesis. the decrease of the level of enzyme activity in the case of pts mutation (glur3 mutant) occurs at the expense of glucose transport disturbance. the mutation cat (tgl) (mutant glur5) causes the increase in enzyme synthesis at the expe ...197897172
biosynthesis of delta-aminolevulinic acid by blue-green algae (cyanobacteria).when levulinic acid, a competitive inhibitor of delta-aminolevulinic acid dehydratase, was added to growing cultures of blue-green algae (cyanobacteria), delta-aminolevulinic acid was excreted into the medium and cell growth was inhibited.197897274
amino acid sequence of beta-galactosidase. viii. sequence of the nh2-terminal segment, cnbr peptides 1 to 9, residues 1 to 377.the amino acids in 9 cyanogen bromide peptides have been placed in sequence starting from the nh2 terminus. the peptides account for residues 1 to 377 of the whole protein and include the largest (cnbr7, 119 residues) and the smallest (cnbr1, 2 residues) of the cyanogen bromide peptides. this region contains only 3 of the 20 lysine residues in the polypeptide chain. a high proportion of charged groups are present (28 of 66 arginine, 28 of 60 glutamic acid, and 24 of 65 aspartic acid residues).197897295
preliminary x-ray crystallographic data on phospholipase c from bacillus cereus. 197897390
increased vasodepressor effect of prostaglandins a1 and e1 in renal hypertensive rabbits. 197897482
malignant transformation of a mouse epithelial cell line with polycyclic aromatic hydrocarbons. 197897564
functional specialisation in the visual cortex of the rhesus monkey.anatomical and functional studies of the visual cortex of the rhesus monkey have shown that it is made up of a multiplicity of distinct areas. these seem to be functionally specialised to analyse different features of the visual environment.197897565
the effect of prolonged dextran administration in guinea pigs.dextran 70 6% in 0.9% nacl solution, injected intraperitoneally in guinea pigs, 10 ml/kg b.w./day, during a period of 27 days brought the lowering of the plasma proteins level, whie the total colloid osmostic pressure (due to dectran and proteins) remained close to that of the control group. the concentration of typhus 0 antibodies and the number of precipitation lines assayed during immunodiffusion in gel were lower than in thd control group. the diminishing of the red blood cells number and of ...197897691
[reservoirs and wild vectors of trypanosoma cruzi. lxviii: infection of mammals by oral route]. 197897735
isolation care of infection-prone burn patients.an experimental and clinical study of spread of colonisation between burn patients, and their susceptibility to infection, was performed. burn patients' polymorphonuclear neutrophil granulocytes (pmn) functioned poorly, particularly during the second week after injury which coincided with maximum growth of bacteria in the burn wound. patients with large burns often dispersed s. aureus and ps. aeruginosa but also beta-hemolytic streptococcus to the air of their rooms. airborne transfer of these b ...197897776
the pattern of sporulation in anabaena circinalis and comments on the role of heterocysts in sporulation in blue-green algae.cells between two intercalary heterocysts differentiate at random into spores in a. circinalis. one or more cells, which fail to transform into spores, are present between the two adjacent spores, and these cells disorganize later. a critical c:n ratio regulates sporulation and heterocyst formation. during sporulation the reductive ability of the heterocyst gradually diminishes. it is concluded on the basis of this and other evidence that sporulation is regulated by interactions between heterocy ...197897869
genetics of the idiotype of strain akr antibodies to group a streptococcal carbohydrate; further evidence for a low degree of homology in the vh chromosomal region.a new vh gene is described which governs the expression of idiotypes associated with the antibody response of strain akr mice to group a streptococcal carbohydrate (a-cho). this vh gene, termed achd, is identified using guinea pig antisera to a pool of strain akr antibodies to a-cho. the genetic polymorphism is revealed by quantitative differences in idiotype expression between different inbred strains. in recombinant inbred strains linkage to the ig-1d allotype allele of strain akr is demonstr ...197897871
activity of three 8-hydroxyquinoline derivatives against in vitro dental plaque.three 8-hydroxyquinoline derivatives, assessed using an in vitro preformed dental plaque model system, were differentially inhibitory for four plaque-forming microorganisms.197898105
characteristics of pr5, a lipid-containing plasmid-dependent phage.an extensive characterization of plasmid-dependent phage pr5 isolated from sewage has been carried out. the phage has a head diameter of 65--68 nm, is isometric with a double-layered capsid, and a minority possess tails. it adsorbs to many but not all types of bacteria possessing p, n, or w plasmids. the phage contains 20% lipid, 15.1% dna, and 64.9% protein by weight and has a buoyant density of 1.265 g/ml in cscl. the dna is double-stranded with a g + c content of 49% and a molecular weight of ...197898225
cellular uptake, transport, and macromolecular binding of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene by human cells in vitro. 197898231
behavioral observations in squirrel monkeys (saimiri sciureus) following methylmercury exposure. 197898322
ultrastructural hepatic changes by carbamate pesticide (sevin) in rats. 197898323
biochemical changes in guinea pig lungs due to amosite asbestos. 197898326
immunopathology of malaria. 197898476
formylation of initiator trna methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression.eucaryotic and procaryotic organisms differ in two aspects of their translation machinery: polycistronic messengers are expressed as a sequence of individual proteins only in procaryotes, and the initiation of protein synthesis proceeds with an initiator trna which is found to be modified (formylated) in procaryotes and not in eucaryotes. in the present study, we show that formylation is required in vivo for the coordinate expression of the escherichia coli lactose operon. our experiments are co ...197898518
ultrastructure of peptidergic neurosecretory axons in the developing neural lobe of the rat. 197886479
transmembrane linkage of fibronectin to intracellular actin-containing filaments in cultured human fibroblasts. 1978291374
vidarabine monophosphate in viral encephalitis. 197986765
interactions of lysophospholipids and mast cells. 197986956
palliation of massive hemoptysis from unresectable carcinoma of the lung.unresectable carcinoma of the lung has traditionally been recognized as a contraindication to surgery in massive hemoptysis. a 60-year-old man had massive hemoptysis. at surgery an unresectable neoplastic mass invading the mediastinum and great vessels was encountered. subtotal resection was accomplished using a stapler (autostapler). the margins of the bronchial and vascular staples were of necessity placed directly through the tumor. the patient had an uneventful recovery and has survived six ...197988310
[spontaneous rosette forming, fc and complement receptor bearing lymphocytes in pediatric diseases].investigations with lymphocyte subpopulations were made in 51 children aged from 1-14 years with infections of the upper airways, with acute leukaemias, and other malignant diseases. t-lymphocytes were registered by means of the spontaneous rosette test. the attempt of proving b-lymphocytes was made by means of an eac test with human erythrocytes, anti-d-immunoglobulins and human complement. the results were compared with those obtained by an eac test with sheep erythrocytes, sheep haemolysin an ...197988386
a simple and sensitive method for the quantitative estimation of collagen. 197988913
revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes. 197990022
cell differentiation in human gastric gland as revealed by nuclear binding of tritiated actinomycin.the nuclear binding of h3 actinomycin, which is closely linked to the differentiation phenomenon, was studied in human normal gastric mucosa. actinomycin binding decreases in cells which differentiate and becomes very low in fully differentiated cells. in the gastric pits, there is a decreasing gradient of labelling from the deeper stem cells to the well-differentiated superficial cells. this indicates that migration and renewal of the surface epithelium occurs following a 'pipe-line' system. al ...197990638
prolonged survival of corneal allografts in rabbits treated with cyclosporin a. 197990771
soluble immune complex disease associated with antigen heterogeneity an hla related disorder.it is suggested that soluble immune complex diseases arising after infections may result from the liberation of partially synthesized bacterial polypeptide or viral nucleic acid antigens. these disrupted antigens will have heterogeneous molecular weights due to antigenic material which is incomplete as a result of premature termination of synthesis. antigens of this type have been shown to result in significant soluble complex formation in vitro when reacted with antisera from many individuals. ...197991086
procaine-induced seizures in epileptic monkeys with bilateral hippocampal foci.intravenous procaine hcl given at low doses (0.5-2.5 mg/kg) to two monkeys with bilateral alumina hippocampal foci depressed interictal spiking or had little effect. at 5.0 mg/kg unilateral limbic activation occurred. at 10.0 mg/kg unilateral or bilateral limbic activation and generalized seizures could be evoked within 3-10 min. at higher doses (15 and 20 mg/kg) bilateral limbic activation or brief (one min) generalized seizures occurred. the unilateral-onset psychomotor seizures were not ident ...197991502
[method of obtaining a y. pestis fraction containing heterogenetic antigens]. 197991715
immunofluorescence testing for chlamydial antibodies. 197991742
viability assessment by dye exclusion. a fluorescent method for fungal cells.a new fluorescent staining technique for fungi utilizes the differential affinity of dead cells for rhodamine b. a mounting medium has been devised that includes the dye. this medium allows direct processing of cells from a broth and provides optimal conditions for fluorescence intensity. comparison with the standard methylene blue exclusion test as applied to the candida albicans yeast phase indicates similar specificity. various pseudohyphae and several fungi also exhibited selective uptake of ...197992216
effects of painful footshock on self-stimulation behaviour of rats and influence of naloxone on this behaviour [proceedings]. 197992231
processing of 19 s thyroglobulin in vitro [proceedings]. 197992247
immunologic regulation in pregnancy.theories to explain the failure of rejection of the fetus by the mother during pregnancy include immunologic privilege of the uterus as a graft site, lack of transplantation antigen expression on the trophoblast, weakening of maternal cellular immunity during pregnancy, and separation of maternal and fetal circulations. evidence for and against each of these theories is discussed. local concentration of a variety of hormones, including human chorionic gonadotropin (hcg), sex steroids, alpha-feto ...197992318
guinea-pig alpha 1 microglobulin. isolation and properties in comparison with human alpha 1 microglobulin. 197992407
ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression.the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ...197992523
[physicochemical properties of neurospecific basic protein (scp) of the spinal cord].basic protein (scp) known as antiencephalytogenic was isolated from the bull spinal cord and spinal roots. the protein is purified to a molecular homogeneous state, some of its physicochemical properties are studied. molecular homogeneity of the protein is established by the method of disk electrophoresis in 15% polyacrylamide gel with sodium dodecyl sulphate present and by analytic ultracentrifugation. during electrophoresis in 15% polyacrylamide gel at ph 4.0 the protein movement occurs only i ...197992837
[determination of the internal association constant as a measure of the affinity of erythocyte-fixed antibodies that interact with mono- and multivalent antigens].the fraction of antibody-bound determinants of a polyvalent antigen is determined by the fraction of free antigen molecules and the valence of the antigen. the internal association constant for antibodies fixed onto erythrocytes can be found by the suspension method, the index obtained in this process being similar when both monovalent and polyvalent antigens are used. the sensitivity of the antibody-erythrocyte diagnostic preparation in the passive hemagglutinin test depends on the affinity of ...197992866
antigenic determinants of horse cytochrome c for lymphocyte transformation: identification of an immunodominant peptide. 197993078
delayed hypersensitivity to staphylococcus aureus in mice: in vivo responses to isolated staphylococcal antigens.the development of delayed hypersensitivity (dh) to staphylococcus aureus in swiss mice was evaluated by the footpad (fp) assay. in order to determine which component of the bacteria was responsible for the in vivo immune reactivity, purified staphylococcal cell wall, cell membrane, protein a, lipoteichoic acid, teichoic acid, as well as lipid-free membrane proteins were isolated. the immune responses of mice receiving one to eight s. aureus injections indicated that the first dh peak, following ...197993087
biosynthesis of human blood group t-, n- and m-specific immunodeterminants on human erythrocyte antigens.we synthesized on tn erythrocytes with human sera, udp-gal, and activators t-specific haptenic structures in satisfactory yield. the specificity of this biosynthesis was ascertained by agglutination with human and animal anti-t, by specific absorption of human anti-t as well as by agglutination inhibition assays. with isolated human erythrocyte t antigen as substrate we synthesized n- and m-specific structures with sera from individual human donors in presence of cmp-sialic acid by incubation fo ...197993134
anomalous alpha 2-macroglobulin-protease complexes in cystic fibrosis: decreased uptake of the complexes by fibroblasts in culture.immunochemical and functional properties of control and cystic fibrosis (cf) alpha 2-macroglobulin (alpha 2m) are compared. crossed immunoelectrophoresis and ouchterlony double diffusion revealed no qualitative differences between the two alpha 2-m preparations. trypsin-esterase activity assayed with bapna as a substrate, in the presence of an excess sti, gave similar ratios between total and active alpha 2m. these alpha 2m-trypsin complexes were equally stable under various experimental conditi ...197993265
effect of azacytidine on simian virus 40 nucleoprotein complexes.simian virus 40 nucleoprotein complexes synthesized in the presence of 5-azacytidine showed small differences in sedimentation rate on neutral sucrose and buoyant density in metrizamide and cesium chloride. simian virus 40 deoxyribonucleic acid (dna) i, isolated from the nucleoprotein complexes of drug-treated cultures, was found to band at a higher buoyant density and therefore had a decreased ability to bind ethidium bromide. the data indicated that these molecules were deficient in superhelic ...197993427
a novel class of schizosaccharomyces pombe mutants, phenotypically unable to grow on glycerol, a respiratory substrate, but still able to oxidize glycerol aerobically [proceedings]. 197993469
antigenic heterogeneity of the non-serogroup antigen structure of neisseria gonorrhoeae lipopolysaccharides.studies of the antigenic structure of the polysaccharide component of gonococcal lipopolysaccaride (lps) indicated that the non-serogroup antigen structure is antigenically heterogeneous. immunodiffusion studies of gc4 strain 8551 indicated that in addition to the gc serogroup determinent, this polysaccharide contains two other sets of determinants, one which is shared with the other five gc serogroups and a second which is shared by the gc1 and gc3 serogroups. enzyme-linked immunosorbent assay ...197993575
genetic variation in the m antigen of group a streptococci: reassortment of type-specific markers and possible antigenic drift.the phylogenetic relationships of 73 strains of group a streptococcus isolated over a six-year period from a population with a high prevalence of streptococcal infections were analyzed with use of four serotype-specific markers: the serum opacity factor (of), the t antigen, the determinant of the m antigen that precipitates with antibody, and the m antiphagocytic determinant. of inhibition tests divided the strains into five subtypes: a, b, c, d, and e. strains within subtypes a, b, and c shared ...197993611
double staining technique for rat foetus skeletons in teratological studies.cartilage and bone were differentiated using alcian blue and alizarin red s respectively. anomalies of both cartilaginous and bony parts of the skeleton could be examined.197993666
1-isoamyl-3-isobutylxanthine: a remarkably potent agent for the potentiation of norepinephrine, histamine, and adenosine-elicited accumulations of cyclic amp in brain slices. 197993674
in vivo release of substance p in the cat substantia nigra.push-pull cannuale were implanted into the substantia nigra (sn) and the caudate nucleus (cn) of the cat to study the in vivo release of substance p (sp), using a radioimmunoassay and high pressure liquid chromatography (hplc) analysis. the spontaneous release of sp could be detected in the sn and the cn. potassium (50 mm) locally applied stimulated sp release in both structures. furthermore an important evoked release of sp was observed in the sn during depolarization of striato-nigral sp fiber ...197993729
in vitro primary immunization of human peripheral blood lymphocytes to klh: evidence for hla-d-region restriction. 197993813
receptive field organization in areas 17 and 18 of the cat [proceedings]. 197993893
mutagenicity of n-nitrosodiethanolamine and its acetyl-derivatives [proceedings]. 197993920
integration site of bovine leukemia virus dna in the bovine genome [proceedings]. 197993923
the effects of pesticin on yersinia enterocolitica and y. pseudotuberculosis. 197994002
a developmental model for studying choline acetyltransferase transport in chick motoneurons.the proximodistal axonal transport of choline acetyltransferase (chac) has been studied in the chick sciatic nerve in the absence of any experimental manipulation. the phenomenon utilized by us is a physiological transient fall in the activity of chac. this fall occurs in most areas of the chick central nervous system after hatching, and moves toward the periphery along the nerves. having ruled out the presence of transient inhibitors, the movement of the fall towards skeletal muscles has been c ...197994010
alien h-2 allospecificities in murine chemically-induced tumors.sera from c3h/heha mice immunized with syngeneic methylcholanthrene-induced sarcoma react with allogeneic thymus, lymphoma and leukemia cells. the presence on leukemia and lymphoma cells of h-2 specificities expressed on normal cells of other h-2 haplotypes from the one in which the tumor originates is described. it was observed that the reaction of antisera to h-2 specificities with lymphoma cells was blocked by anti-mca sarcoma sera. the cross-reactivity between mc sarcomas, thymus, leukemia a ...197994037
demonstration of specific antigenecity of various fractions of human liver homogenate. 197994039
antigenicity of casein enzymatic hydrolysate.an enzymatic hydrolysate of bovine casein were studied for molecular size and antigenic properties. as determined by sephadex g-25 gel filtration, polypeptide fractions having molecular weights greater than 1,000 were not detected in the soluble fraction of the casein hydrolysate. antigenicity of the fraction was not detected by heterologous passive cutaneous anaphylaxis in guinea pig-rabbit antiserum system mediated by immunoglobulin g types of antibody (guinea pig passive cutaneous anaphylaxis ...197994065
studies on the oxidation of muscle triglycerides. 197994070
[criterion of the comparative sensitivity of plague antigenic and antibody erythrocyte diagnostica in detecting antibodies]. 197994132
[electrophoretic mobility test in the early diagnosis of malignant tumors; findings in the prestages of cervical and breast cancers].a double-blind pilot study of 130 female patients was carried out to determine the feasibility and significance of the em test in the early diagnosis of carcinoma of the female genital organs and breast. early stages of cervical carcinoma (carcinoma in situ) as well as fibroid adenoma, mastopathy and breast tumors were tested and compared with the results of their manifest forms. positive results were recorded in 87.5% of the cases of middle-grade to severe epithelial dysplasia (papanicolaou iii ...197994155
[clinical and biochemical contributions on the effect of maprotiline and clomipramine in depressive states (preliminary observation)].this study has been conducted on 17 patients suffering from neurotic and psychotic depressions, who had not received a pharmacological antidepressive therapy since at least 1 month. 6 patients suffering from psychotic depression were treated with 100 mg clomipramine i.v. daily for 20 days; 11 patients, 7 of whom suffered from psychotic depression and 4 from neurotic depression, were treated with 150 mg maprotiline i.v. daily for 20 days. in both groups the therapy has been continued orally. in ...197994169
[vascularization of malignant melanoma in golden hamster]. 197994247
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