Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| possible translocation of periodontal pathogens into the lymph nodes draining the oral cavity. | numerous publications have reported the presence of periodontopathogenic bacteria in peripheral and central vascular lesions. however, it is unclear how this bacterial translocation occurs. the objective of this study was to investigate whether periodontopathic bacteria are translocated to lymph nodes proximal to the oral cavity. obtaining lymph node samples is not ethically feasible unless they are excised as part of the surgical management of patients with cancer. this study analyzed formalin- ... | 2012 | 23124752 |
| reduction of salivary arginine catabolic activity through periodontal therapy. | salivary enzymes may be used to diagnose periodontal conditions. salivary arginase activity (saa) is related to susceptibility to bacterial infection. therefore, the aim of this controlled interventional study was to determine the saa before and after non-surgical periodontal therapy. | 2012 | 23041992 |
| detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women. | the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ... | 2012 | 23018230 |
| quantification of periodontopathic bacteria in saliva using the invader assay. | when quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. the invader assay is a convenient method because it does not involve gene amplification. the purpose of this study was to evaluate the validity of the invader assay to quantify periodontopathic bacteria. the invader technology was applied in quantifying five periodontopathic bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsy ... | 2012 | 22996215 |
| do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens? | the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens, and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures. | 2012 | 22988660 |
| [amalgam, composite and compomer: microbiological study]. | restorative materials have different consequences on the periodontium. the surface of these materials may influence gingival health and cause in some instances gingival inflammation. the purpose of this study was to investigate and compare, in a healthy periodontium, intracrevicular plaque bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis and treponema denticola), at day 0 and at 6 months, around subgingivally located amalga ... | 2012 | 22715643 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| detection of periodontal bacteria in thrombi of patients with acute myocardial infarction by polymerase chain reaction. | numerous reports have demonstrated that periodontal bacteria are present in plaques from atherosclerotic arteries. although periodontitis has recently been recognized as a risk factor for coronary artery disease, the direct relationship between periodontal bacteria and coronary artery disease has not yet been clarified. it has been suggested that these bacteria might contribute to inflammation and plaque instability. we assumed that if periodontal bacteria induce inflammation of plaque, the bact ... | 2012 | 22305832 |
| molecular analysis of 16s rrna genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. | small subunit rrna sequencing and phylogenetic analysis were used to identify cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites. | 2012 | 22326171 |
| anaerobic and microaerophilic pathogens in the biofilm formation on voice prostheses: a pilot study. | voice rehabilitation with voice prostheses is a standard therapy in laryngectomized patients. biofilm formation on the surface of the voice prostheses causes device failure and requires frequent replacements. studies analyzing the biofilm of voice prostheses have mainly focused on aerobic bacteria. anaerobic bacteria as an integral part of the biofilms on voice prostheses have not been investigated yet. | 2012 | 22374743 |
| comparison of endotoxin levels found in primary and secondary endodontic infections. | this clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [lpss]) found in primary and secondary endodontic infections with apical periodontitis by correlating lps contents with clinical/radiographic findings. in addition, the presence of target gram-negative anaerobic bacteria was also investigated. | 2012 | 22794210 |
| structural, bioinformatic, and in vivo analyses of two treponema pallidum lipoproteins reveal a unique trap transporter. | treponema pallidum, the bacterial agent of syphilis, is predicted to encode one tripartite atp-independent periplasmic transporter (trap-t). trap-ts typically employ a periplasmic substrate-binding protein (sbp) to deliver the cognate ligand to the transmembrane symporter. herein, we demonstrate that the genes encoding the putative trap-t components from t. pallidum, tp0957 (the sbp), and tp0958 (the symporter), are in an operon with an uncharacterized third gene, tp0956. we determined the cryst ... | 2012 | 22306465 |
| screening for periodontopathogenic bacteria in severe chronic periodontitis in a moroccan population. | bacteria play an important role in destructive periodontitis. the aim of this study was to screen for five highly pathogenic bacteria: aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, and treponema denticola, in moroccan patients presenting with severe chronic periodontitis and to compare results with those of patients presenting with severe aggressive periodontitis. | 2012 | 23141872 |
| differential proteomic analysis of a polymicrobial biofilm. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. the aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. in a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture p. gingivalis and t. denticola were closely associated and dominated the polymicrobial biofilm. for comparison, ... | 2012 | 22808953 |
| effects of systemic sitafloxacin on periodontal infection control in elderly patients. | to evaluate the microbiological and clinical effects of the systemic administration of sitafloxacin (stfx) on periodontal pockets in elderly patients receiving supportive periodontal therapy (spt). | 2012 | 22616908 |
| [relationship between the preterm low birth weight infant and the periodontal pathogen bacteria in maternal saliva]. | to investigate the possible relationship between the preterm and/or low birth weight (plbw) and three anaerobic microorganisms in saliva of their mothers, including porphyromonas gingivalis (pg), tannerella forsythia (tf), treponema denticola (td). | 2012 | 22353895 |
| structural insights into catalysis by βc-s lyase from streptococcus anginosus. | hydrogen sulfide (h(2)s) is a causative agent of oral malodor and may play an important role in the pathogenicity of oral bacteria such as streptococcus anginosus. in this microorganism, h(2)s production is associated with βc-s lyase (lcd) encoded by lcd gene, which is a pyridoxal 5'-phosphate (plp)-dependent enzyme that catalyzes the α,β-elimination of sulfur-containing amino acids. when lcd acts on l-cysteine, h(2)s is produced along with pyruvate and ammonia. to understand the h(2)s-producing ... | 2012 | 22674431 |
| the fate of treponema denticola within human gingival epithelial cells. | treponema denticola is one of the major pathogens associated with chronic periodontitis. bacterial invasion into gingival tissues is a critical process in the pathogenesis of periodontal disease. we recently reported that t. denticola can invade gingival epithelial cells. the aim of this study is to determine the fate of internalized t. denticola in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with 5- (and 6-) carboxy-fluorescein diacetate succini ... | 2012 | 23134612 |
| biochemical and structural characterization of the trans-enoyl-coa reductase from treponema denticola. | the production of fatty acids is an important cellular pathway for both cellular function and the development of engineered pathways for the synthesis of advanced biofuels. despite the conserved reaction chemistry of various fatty acid synthase systems, the individual isozymes that catalyze these steps are quite diverse in their structural and biochemical features and are important for controlling differences at the cellular level. one of the key steps in the fatty acid elongation cycle is the e ... | 2012 | 22906002 |
| growth curves and morphology of three treponema subtypes isolated from digital dermatitis in cattle. | digital dermatitis (dd) is an infectious claw disease of cattle that causes painful lesions, principally along the coronary band of the claws. in the us alone, the estimated economic impact of dd is estimated to be $190 million. the etiology of dd remains unclear and there is no reliable laboratory test, so dd is most often diagnosed clinically. spirochetal bacteria of the genera treponema have been implicated in dd infections following their isolation using culture techniques, serological detec ... | 2012 | 22901455 |
| identification of four treponema species in subgingival samples by nested-pcr and their correlation with clinical diagnosis. | the relationship between different species of oral treponemas and inflammation in periodontal disease progression is complex. the purpose of this study was to analyze and compare the subgingival plaque samples collected from periodontally healthy subjects and from chronic gingivitis and periodontitis patients in order to detect the presence of t. denticola, t. pectinovorum, t. socranskii and t. vincentii using nested-pcr technology. after dna extraction from the samples using qiamp dna mini kit ... | 2012 | 22838219 |
| integrin α5β1 activates the nlrp3 inflammasome by direct interaction with a bacterial surface protein. | integrins are cell-surface heterodimeric glycoproteins composed of alpha and beta subunits that mediate cell-cell, cell-extracellular matrix, and cell-pathogen interactions. in this study, we report a specific role of integrin α5β1 in nlrp3 inflammasome activation in macrophages stimulated by td92, a surface protein of the periodontopathogen, treponema denticola. the direct interaction of td92 with the cell membrane integrin α5β1 resulted in atp release and k(+) efflux, which are the main events ... | 2012 | 22608495 |
| identification of treponema denticola in subgingival samples by pcr technology and its correlation with clinical diagnosis. | treponema denticola has been associated with gingivitis and chronic periodontitis. the aim of this study was to identify treponema denticola in subgingival samples using pcr technology and to correlate it with clinical diagnosis of subjects. the study was carried out on seventy patients (20-84 years of age; mean age, 45.06 +/- 12.58) of which 22 individuals with no detectable gingivitis or periodontitis, 4 subjects with chronic gingivitis and 44 subjects with chronic periodontitis. subgingival p ... | 2012 | 22568260 |
| prevalence, biogenesis, and functionality of the serine protease autotransporter espp. | enterohemorrhagic e. coli (ehec) causes severe diseases in humans worldwide. one of its virulence factors is espp, which belongs to the serine protease autotransporters of enterobacteriaceae (spate) family. in this review we recapitulate the current data on prevalence, biogenesis, structural properties and functionality. espp has been used to investigate mechanistic details of autotransport, and recent studies indicate that this transport mechanism is not autonomous but rather dependent on addit ... | 2012 | 23274272 |
| prevalence, biogenesis, and functionality of the serine protease autotransporter espp. | enterohemorrhagic e. coli (ehec) causes severe diseases in humans worldwide. one of its virulence factors is espp, which belongs to the serine protease autotransporters of enterobacteriaceae (spate) family. in this review we recapitulate the current data on prevalence, biogenesis, structural properties and functionality. espp has been used to investigate mechanistic details of autotransport, and recent studies indicate that this transport mechanism is not autonomous but rather dependent on addit ... | 2012 | 23274272 |
| metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study. | this study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. dna was extracted from subgingival samples and subjected to pcr amplification of the c2-c4 regions of the 16s rdna using primers with bar codes to identify individual samples. the pcr products were pooled and sequenced for the v4 region of the 16s rdna using the 454 flx standard platform. the results were analyzed f ... | 2012 | 23341849 |
| structure of the essential diversity-generating retroelement protein bavd and its functionally important interaction with reverse transcriptase. | diversity-generating retroelements (dgrs) are the only known source of massive protein sequence variation in prokaryotes. these elements transfer coding information from a template region (tr) through an rna intermediate to a protein-encoding variable region. this retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical bpp-1 dgr, requires a reverse transcriptase (brt) and an accessory variability determinant (bavd) protein. to understand the role of bav ... | 2012 | 23273427 |
| structure of the essential diversity-generating retroelement protein bavd and its functionally important interaction with reverse transcriptase. | diversity-generating retroelements (dgrs) are the only known source of massive protein sequence variation in prokaryotes. these elements transfer coding information from a template region (tr) through an rna intermediate to a protein-encoding variable region. this retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical bpp-1 dgr, requires a reverse transcriptase (brt) and an accessory variability determinant (bavd) protein. to understand the role of bav ... | 2012 | 23273427 |
| regulation of tlr2-mediated tolerance and cross-tolerance through irak4 modulation by mir-132 and mir-212. | innate immune response is the first defense against pathogens via recognition by various conserved pattern recognition receptors, such as tlrs, to initiate a rapid and strong cytokine alarm. tlr signaling-mediated cytokine production must be properly regulated to prevent pathological conditions deriving from overproduction of cytokines. in this study, the role of specific micrornas in tlr-signaling pathway was investigated to reveal the cross-interaction and -regulation in the myd88 pathway. in ... | 2012 | 23264652 |
| regulation of tlr2-mediated tolerance and cross-tolerance through irak4 modulation by mir-132 and mir-212. | innate immune response is the first defense against pathogens via recognition by various conserved pattern recognition receptors, such as tlrs, to initiate a rapid and strong cytokine alarm. tlr signaling-mediated cytokine production must be properly regulated to prevent pathological conditions deriving from overproduction of cytokines. in this study, the role of specific micrornas in tlr-signaling pathway was investigated to reveal the cross-interaction and -regulation in the myd88 pathway. in ... | 2012 | 23264652 |
| conservation and revised annotation of the treponema denticola prcb-prca-prtp locus encoding the dentilisin (ctlp) protease complex. | interstrain differences in antigenic surface proteins may reflect immunological pressure or differences in receptor specificity of the antigen. treponema denticola exhibits considerable interstrain variability in its major surface protein (msp), but no studies have addressed this issue in dentilisin (ctlp), a surface protease complex that has a significant role in t. denticola-host interactions in periodontal disease. furthermore, the genome annotation of the prcb-prca-prtp operon encoding denti ... | 2012 | 23253337 |
| conservation and revised annotation of the treponema denticola prcb-prca-prtp locus encoding the dentilisin (ctlp) protease complex. | interstrain differences in antigenic surface proteins may reflect immunological pressure or differences in receptor specificity of the antigen. treponema denticola exhibits considerable interstrain variability in its major surface protein (msp), but no studies have addressed this issue in dentilisin (ctlp), a surface protease complex that has a significant role in t. denticola-host interactions in periodontal disease. furthermore, the genome annotation of the prcb-prca-prtp operon encoding denti ... | 2012 | 23253337 |
| microbial interactions in building of communities. | establishment of a community is considered to be essential for microbial growth and survival in the human oral cavity. biofilm communities have increased resilience to physical forces, antimicrobial agents and nutritional variations. specific cell-to-cell adherence processes, mediated by adhesin-receptor pairings on respective microbial surfaces, are able to direct community development. these interactions co-localize species in mutually beneficial relationships, such as streptococci, veillonell ... | 2012 | 23253299 |
| microbial interactions in building of communities. | establishment of a community is considered to be essential for microbial growth and survival in the human oral cavity. biofilm communities have increased resilience to physical forces, antimicrobial agents and nutritional variations. specific cell-to-cell adherence processes, mediated by adhesin-receptor pairings on respective microbial surfaces, are able to direct community development. these interactions co-localize species in mutually beneficial relationships, such as streptococci, veillonell ... | 2012 | 23253299 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| papillon-lefèvre syndrome: a series of six cases in the same family. | papillon-lefèvre syndrome (pls) is a rare, autosomal recessive heterogeneous disorder, which is characterized by palmoplantar hyperkeratosis, early loss of primary and permanent teeth, and associated calcification of the dura mater. herein we described six cases of pls in the same family. in this series, six cases (two females and four males) with the mean age of 15.6 ± 10.4 years were recruited. palmoplantar hyperkeratosis was detected in all of the cases, leading to a difficult and painful wal ... | 2012 | 23251811 |
| optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens. | the aim of this study was to optimize quantitative (real-time) polymerase chain reaction (qpcr) assays for 8 major periodontal pathogens, i.e. aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, parvimonas micros, porphyromonas gingivalis, prevotella intermedia, tanerella forsythia and treponema denticola, and of the caries pathogen streptococcus mutans. | 2012 | 23199017 |
| biophysical and bioinformatic analyses implicate the treponema pallidum tp34 lipoprotein (tp0971) in transition metal homeostasis. | metal ion homeostasis is a critical function of many integral and peripheral membrane proteins. the genome of the etiologic agent of syphilis, treponema pallidum, is compact and devoid of many metabolic enzyme genes. nevertheless, it harbors genes coding for homologs of several enzymes that typically require either iron or zinc. the product of the tp0971 gene of t. pallidum, designated tp34, is a periplasmic lipoprotein that is thought to be tethered to the inner membrane of this organism. previ ... | 2012 | 23042995 |
| il-17 and il-11 gcf levels in aggressive and chronic periodontitis patients: relation to pcr bacterial detection. | this study evaluated il-17 and il-11 in gingival crevicular fluid (gcf) of generalized chronic periodontitis (gcp) and generalized aggressive periodontitis (gagp) patients in relation to periodontopathic bacteria. | 2012 | 23226926 |
| comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. | persistence of microorganisms or reinfections are the main reasons for failure of root canal therapy. very few studies to date have included culture-independent methods to assess the microbiota, including non-cultivable microorganisms. the aim of this study was to combine culture methods with culture-independent cloning methods to analyze the microbial flora of root-filled teeth with periradicular lesions. twenty-one samples from previously root-filled teeth were collected from patients with per ... | 2012 | 23152922 |
| development of microorganisms for cellulose-biofuel consolidated bioprocessings: metabolic engineers' tricks. | cellulose waste biomass is the most abundant and attractive substrate for "biorefinery strategies" that are aimed to produce high-value products (e.g. solvents, fuels, building blocks) by economically and environmentally sustainable fermentation processes. however, cellulose is highly recalcitrant to biodegradation and its conversion by biotechnological strategies currently requires economically inefficient multistep industrial processes. the need for dedicated cellulase production continues to ... | 2012 | 24688667 |
| proteome variation among filifactor alocis strains. | filifactor alocis, a gram-positive anaerobic rod, is now considered one of the marker organisms associated with periodontal disease. although there was heterogeneity in its virulence potential, this bacterium was shown to have virulence properties that may enhance its ability to survive and persist in the periodontal pocket. to gain further insight into a possible mechanism(s) of pathogenesis, the proteome of f. alocis strains was evaluated. proteins including several proteases, neutrophil-activ ... | 2012 | 23008013 |
| cleavage of igg1 in gingival crevicular fluid is associated with the presence of porphyromonas gingivalis. | immunoglobulin (ig) g1 plays an important role in the adaptive immune response. kgp, a lysine-specific cysteine protease from porphyromonas gingivalis, specifically hydrolyses igg1 heavy chains. the purpose of this study was to examine whether cleavage of igg1 occurs in gingival crevicular fluid (gcf) in vivo, and whether there is any association with the presence of porphyromonas gingivalis and other periodontopathogens. | 2012 | 23116446 |
| cleavage of igg1 in gingival crevicular fluid is associated with the presence of porphyromonas gingivalis. | immunoglobulin (ig) g1 plays an important role in the adaptive immune response. kgp, a lysine-specific cysteine protease from porphyromonas gingivalis, specifically hydrolyses igg1 heavy chains. the purpose of this study was to examine whether cleavage of igg1 occurs in gingival crevicular fluid (gcf) in vivo, and whether there is any association with the presence of porphyromonas gingivalis and other periodontopathogens. | 2012 | 23116446 |
| bana-positive plaque samples are associated with oral hygiene practices and not cd4+ t cell counts in hiv-positive patients. | background. the "red complex" microorganisms, namely, porphyromonas gingivalis, treponema denticola, and tannerella forsythia are considered as potential pathogens causing hiv-associated periodontal diseases. moreover, it has been recognized that an association exists between cd4+ t cell counts and periodontal disease progression. objective. to establish whether cd4+ t cell counts or oral hygiene plays a greater role in producing bana-positive results in hiv-associated periodontal disease. mater ... | 2012 | 23258979 |
| analysis of thic variants in the context of the metabolic network of salmonella enterica. | in bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (hmp) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (air), a branch point metabolite of purine and thiamine biosynthesis. thic is a member of the radical s-adenosylmethionine (adomet) superfamily and catalyzes the complex chemical rearrangement of air to hmp-p. as reconstituted in vitro, the thic reaction requires adomet, air, and reductant. this study analyzed variants of thic in vivo and in vitro to probe the metaboli ... | 2012 | 22961850 |
| complement and dysbiosis in periodontal disease. | signaling crosstalk between complement and toll-like receptors (tlrs) normally serves to coordinate host immunity. however, the periodontal bacterium porphyromonas gingivalis expresses c5 convertase-like enzymatic activity and adeptly exploits complement-tlr crosstalk to subvert host defenses and escape elimination. intriguingly, this defective immune surveillance leads to the remodeling of the periodontal microbiota to a dysbiotic state that causes inflammatory periodontitis. understanding the ... | 2012 | 22964237 |
| a phage display selected 7-mer peptide inhibitor of the tannerella forsythia metalloprotease-like enzyme karilysin can be truncated to ser-trp-phe-pro. | tannerella forsythia is a gram-negative bacteria, which is strongly associated with the development of periodontal disease. karilysin is a newly identified metalloprotease-like enzyme, that is secreted from t. forsythia. karilysin modulates the host immune response and is therefore considered a likely drug target. in this study peptides were selected towards the catalytic domain from karilysin (kly18) by phage display. the peptides were linear with low micromolar binding affinities. the two best ... | 2012 | 23119051 |
| offense and defense: microbial membrane vesicles play both ways. | microbes have evolved over millennia to become adapted and specialized to the environments that they occupy. these environments may include water or soil, extreme environments such as hydrothermal vents, and can even include a host organism. to become adapted to these locations, microbes have evolved specific tools to mediate interactions with the environment. one such tool that prokaryotes have evolved includes the production of membrane vesicles (mvs). mvs are 10-300 nm spherical blebs derived ... | 2012 | 23123555 |
| controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways. | microbial systems are being increasingly developed as production hosts for a wide variety of chemical compounds. broader adoption of microbial synthesis is hampered by a limited number of high-yielding natural pathways for molecules with the desired physical properties, as well as the difficulty in functionally assembling complex biosynthetic pathways in heterologous hosts. here, we address both of these challenges by reporting the adaptation of the butanol biosynthetic pathway for the synthesis ... | 2012 | 23071297 |
| glycobiology aspects of the periodontal pathogen tannerella forsythia. | glycobiology is important for the periodontal pathogen tannerella forsythia, affecting the bacterium's cellular integrity, its life-style, and virulence potential. the bacterium possesses a unique gram-negative cell envelope with a glycosylated surface (s-) layer as outermost decoration that is proposed to be anchored via a rough lipopolysaccharide. the s-layer glycan has the structure 4‑meo-b-manpnacconh2-(1→3)-[pse5am7gc-(2→4)-]-b-manpnaca-(1→4)-[4-meo-a-galp-(1→2)-]-a-fucp-(1→4)-[-a-xylp-(1→3 ... | 2012 | 24970146 |
| quantitative discrimination of aggregatibacter actinomycetemcomitans highly leukotoxic jp2 clone from non-jp2 clones in diagnosis of aggressive periodontitis. | aggregatibacter actinomycetemcomitans is the etiological agent of periodontitis, and there is a strong association between clone jp2 and aggressive periodontitis in adolescents of african descent. the jp2 clone has an approximately 530-bp deletion (∆530) in the promoter region of the lkt/ltx gene, which encodes leukotoxin, and this clone has high leukotoxic activity. therefore, this clone is very important in aggressive periodontitis. to diagnose this disease, culture methods and conventional pc ... | 2012 | 23050598 |
| advancement of the 10-species subgingival zurich biofilm model by examining different nutritional conditions and defining the structure of the in vitro biofilms. | periodontitis is caused by a highly complex consortium of bacteria that establishes as biofilms in subgingival pockets. it is a disease that occurs worldwide and its consequences are a major health concern. investigations in situ are not possible and the bacterial community varies greatly between patients and even within different loci. due to the high complexity of the consortium and the availability of samples, a clear definition of the pathogenic bacteria and their mechanisms of pathogenicity ... | 2012 | 23040057 |
| recording and surveillance systems for periodontal diseases. | this paper describes tools used to measure periodontal diseases and the integration of these tools into surveillance systems. tools to measure periodontal diseases at the surveillance level have focussed on current manifestations of disease (e.g. gingival inflammation) or disease sequelae (e.g. periodontal pocket depth or loss of attachment). all tools reviewed in this paper were developed based on the state of the science of the pathophysiology of periodontal disease at the time of their design ... | 2012 | 22909105 |
| miswak: a periodontist's perspective. | meticulous plaque control on a daily routine basis is the single most important step to achieve good oral health. herbal chewing sticks, commonly known as miswak, are among the ancient and traditional oral hygiene aids popular in india, pakistan, most of the arabian countries, and several african countries. but nowadays, because of low cost, free availability, unique chemical composition, and spiritual beliefs, miswak is being used worldwide. a large number of studies have proved that miswak is ... | 2012 | 23326088 |
| sensing the enemy: new role for a bacterial secretion system in activation of an innate immunity-associated microrna. | 2012 | 23076273 | |
| the prosthodontic management of a young edentulous patient with the papillon lefevre syndrome-a rare case report. | the papillon lefevre syndrome is an autosomal recessive disorder which is characterized by various dermatological manifestations and periodontitis as a manifestation of a systemic disease. its aetiopathogenesis is thought to be secondary to the mutation of the cathepsin c gene. various studies have shown that the immune related cells like the polymorphonuclear leucocytes and the macrophages and their precursors were affected.this report elicits about an edentulous male with this syndrome, who wa ... | 2012 | 23373060 |
| a bacterial glycan core linked to surface (s)-layer proteins modulates host immunity through th17 suppression. | tannerella forsythia is a pathogen implicated in periodontitis, an inflammatory disease of the tooth-supporting tissues often leading to tooth loss. this key periodontal pathogen is decorated with a unique glycan core o-glycosidically linked to the bacterium's proteinaceous surface (s)-layer lattice and other glycoproteins. herein, we show that the terminal motif of this glycan core acts to modulate dendritic cell effector functions to suppress t-helper (th)17 responses. in contrast to the wild- ... | 2012 | 22968422 |
| a bacterial glycan core linked to surface (s)-layer proteins modulates host immunity through th17 suppression. | tannerella forsythia is a pathogen implicated in periodontitis, an inflammatory disease of the tooth-supporting tissues often leading to tooth loss. this key periodontal pathogen is decorated with a unique glycan core o-glycosidically linked to the bacterium's proteinaceous surface (s)-layer lattice and other glycoproteins. herein, we show that the terminal motif of this glycan core acts to modulate dendritic cell effector functions to suppress t-helper (th)17 responses. in contrast to the wild- ... | 2012 | 22968422 |
| beyond the red complex and into more complexity: the polymicrobial synergy and dysbiosis (psd) model of periodontal disease etiology. | recent advancements in the periodontal research field are consistent with a new model of pathogenesis according to which periodontitis is initiated by a synergistic and dysbiotic microbial community rather than by select 'periopathogens', such as the 'red complex'. in this polymicrobial synergy, different members or specific gene combinations within the community fulfill distinct roles that converge to shape and stabilize a disease-provoking microbiota. one of the core requirements for a potenti ... | 2012 | 23134607 |
| the keystone-pathogen hypothesis. | recent studies have highlighted the importance of the human microbiome in health and disease. however, for the most part the mechanisms by which the microbiome mediates disease, or protection from it, remain poorly understood. the keystone-pathogen hypothesis holds that certain low-abundance microbial pathogens can orchestrate inflammatory disease by remodelling a normally benign microbiota into a dysbiotic one. in this opinion article, we critically assess the available literature that supports ... | 2012 | 22941505 |
| a proteomic investigation of fusobacterium nucleatum alkaline-induced biofilms. | the gram negative anaerobe fusobacterium nucleatum has been implicated in the aetiology of periodontal diseases. although frequently isolated from healthy dental plaque, its numbers and proportion increase in plaque associated with disease. one of the significant physico-chemical changes in the diseased gingival sulcus is increased environmental ph. when grown under controlled conditions in our laboratory, f. nucleatum subspecies polymorphum formed mono-culture biofilms when cultured at ph 8.2. ... | 2012 | 22943491 |
| energy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from tannerella forsythensis using microwave or autoclave heating with citraconic anhydride. | tannerella forsythensis (bacteroides forsythus), an anaerobic gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. it is characterized by two unique protein bands with molecular weights of more than 200 kda. it also is known to have a typical surface layer (s-layer) consisting of regularly arrayed subunits outside the outer membrane. we examined the relationship between high molecular weight proteins and the s-laye ... | 2012 | 22984898 |
| porphyromonas gingivalis as a potential community activist for disease. | an extensive analysis of dental plaque samples over the years has led to the identification of "red" complex oral bacteria that have a strong association with each other and with disease. consequently, these bacteria have been labeled 'periopathogens'. studies with one of these bacteria, porphyromonas gingivalis, have revealed that it contains several different mechanisms which either impede or modulate periodontal protective mechanisms. in a mouse model of periodontitis, it has been shown that ... | 2012 | 22772362 |
| hcpr of porphyromonas gingivalis is required for growth under nitrosative stress and survival within host cells. | although the gram-negative, anaerobic periodontopathogen porphyromonas gingivalis must withstand nitrosative stress, which is particularly high in the oral cavity, the mechanisms allowing for protection against such stress are not known in this organism. in this study, microarray analysis of p. gingivalis transcriptional response to nitrite and nitric oxide showed drastic upregulation of the pg0893 gene coding for hybrid cluster protein (hcp), which is a putative hydroxylamine reductase. althoug ... | 2012 | 22778102 |
| global expression profile of biofilm resistance to antimicrobial compounds in the plant-pathogenic bacterium xylella fastidiosa reveals evidence of persister cells. | investigations of biofilm resistance response rarely focus on plant-pathogenic bacteria. since xylella fastidiosa is a multihost plant-pathogenic bacterium that forms biofilm in the xylem, the behavior of its biofilm in response to antimicrobial compounds needs to be better investigated. we analyzed here the transcriptional profile of x. fastidiosa subsp. pauca in response to inhibitory and subinhibitory concentrations of copper and tetracycline. copper-based products are routinely used to contr ... | 2012 | 22730126 |
| association of cd14-260 polymorphisms, red-complex periodontopathogens and gingival crevicular fluid cytokine levels with cyclosporine a-induced gingival overgrowth in renal transplant patients. | backgroud and objective: genetic factors may influence the colonization of pathogenic bacteria, therefore increasing the risk for the initiation and development of periodontal disease. the present study was carried out to investigate the association of cd14-260 polymorphisms, subgingival microbiota, and gingival crevicular fluid (gcf) cytokine levels with cyclosporine a (csa)-induced gingival overgrowth (go) in renal transplant patients. | 2012 | 22934794 |
| association of cd14-260 polymorphisms, red-complex periodontopathogens and gingival crevicular fluid cytokine levels with cyclosporine a-induced gingival overgrowth in renal transplant patients. | backgroud and objective: genetic factors may influence the colonization of pathogenic bacteria, therefore increasing the risk for the initiation and development of periodontal disease. the present study was carried out to investigate the association of cd14-260 polymorphisms, subgingival microbiota, and gingival crevicular fluid (gcf) cytokine levels with cyclosporine a (csa)-induced gingival overgrowth (go) in renal transplant patients. | 2012 | 22934794 |
| bacterial cell surface heterogeneity: a pathogen's disguise. | 2012 | 22952444 | |
| bugs that debugs: probiotics. | the oral cavity harbors a diverse array of bacterial species. there are more than 600 species that colonize in the oral cavity. these include a lot of organisms that are not commonly known to reside in the gastrointestinal (gi) tract and also are more familiar: lactobacillus acidophilus, lactobacillus casei, lactobacillus fermentum, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius. the balance of all these microorganisms can easily be disturbed and a prevalence of p ... | 2012 | 23066281 |
| staphylococcal superantigen-like protein 3 binds to the toll-like receptor 2 extracellular domain and inhibits cytokine production induced by staphylococcus aureus, cell wall component, or lipopeptides in murine macrophages. | staphylococcal superantigen-like proteins (ssls) are a family of exoproteins sharing structural similarity with superantigens, but no superantigenic activity. corresponding host target proteins or receptors against a portion of ssls in the family have been identified. in this study, we show that ssl3 specifically binds to toll-like receptor 2 (tlr2) and inhibits the stimulation of macrophages by tlr2 ligands. an approximately 100-kda protein was recovered by using recombinant his-tagged ssl3-con ... | 2012 | 22665377 |
| oral spirochetes implicated in dental diseases are widespread in normal human subjects and carry extremely diverse integron gene cassettes. | the nih human microbiome project (hmp) has produced several hundred metagenomic data sets, allowing studies of the many functional elements in human-associated microbial communities. here, we survey the distribution of oral spirochetes implicated in dental diseases in normal human individuals, using recombination sites associated with the chromosomal integron in treponema genomes, taking advantage of the multiple copies of the integron recombination sites (repeats) in the genomes, and using a ta ... | 2012 | 22635997 |
| bacterial community shift in treated periodontitis patients revealed by ion torrent 16s rrna gene amplicon sequencing. | periodontitis, one of the most common diseases in the world, is caused by a mixture of pathogenic bacteria and inflammatory host responses and often treated by antimicrobials as an adjunct to scaling and root planing (srp). our study aims to elucidate explorative and descriptive temporal shifts in bacterial communities between patients treated by srp alone versus srp plus antibiotics. this is the first metagenomic study using an ion torrent personal genome machine (pgm). eight subgingival plaque ... | 2012 | 22870235 |
| exploration of the active site of escherichia coli cystathionine γ-synthase. | cystathionine γ-synthase (cgs) catalyzes the condensation of o-succinyl-l-homoserine (l-oshs) and l-cysteine (l-cys), to produce l-cystathionine (l-cth) and succinate, in the first step of the bacterial transsulfuration pathway. in the absence of l-cys, the enzyme catalyzes the futile α,γ-elimination of l-oshs, yielding succinate, α-ketobutyrate, and ammonia. a series of 16 site-directed variants of escherichia coli cgs (ecgs) was constructed to probe the roles of active-site residues d45, y46, ... | 2012 | 22855027 |
| movement of dna sequence recognition domains between non-orthologous proteins. | comparisons of proteins show that they evolve through the movement of domains. however, in many cases, the underlying mechanisms remain unclear. here, we observed the movements of dna recognition domains between non-orthologous proteins within a prokaryote genome. restriction-modification (rm) systems, consisting of a sequence-specific dna methyltransferase and a restriction enzyme, contribute to maintenance/evolution of genomes/epigenomes. rm systems limit horizontal gene transfer but are thems ... | 2012 | 22821560 |
| antibacterial effects of blackberry extract target periodontopathogens. | antimicrobial agents provide valuable adjunctive therapy for the prevention and the control of oral diseases. limitations in their prolonged use have stimulated the search for new, naturally occurring agents with more specific activity and fewer adverse effects. here we sought to determine the antibacterial properties of blackberry extract (bbe) in vitro against oral bacterial commensals and periodontopathogens. | 2012 | 22812456 |
| antibacterial effects of blackberry extract target periodontopathogens. | antimicrobial agents provide valuable adjunctive therapy for the prevention and the control of oral diseases. limitations in their prolonged use have stimulated the search for new, naturally occurring agents with more specific activity and fewer adverse effects. here we sought to determine the antibacterial properties of blackberry extract (bbe) in vitro against oral bacterial commensals and periodontopathogens. | 2012 | 22812456 |
| pathogenicity of treponema denticola wild-type and mutant strain tested by an active mode of periodontal infection using microinjection. | the available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. we developed an active mode of oral infection, using microinjection in the marginal gingiva of mice, to test the pathogenicity of a genetically engineered treponema denticola mutant strain deficient in intermediate-like filaments, compared to the wild-type strain. this targeted ... | 2012 | 22829826 |
| comparative evaluation of 0.1% turmeric mouthwash with 0.2% chlorhexidine gluconate in prevention of plaque and gingivitis: a clinical and microbiological study. | the aim of our clinical trial was to assess the efficacy of 0.1% turmeric mouthwash as an anti-plaque agent and its effect on gingival inflammation and to compare it with 0.2% chlorhexidine gluconate by evaluating the effect on plaque and gingival inflammation and on microbial load. | 2012 | 23162334 |
| fusobacterium nucleatum and tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. | tannerella forsythia is strongly associated with chronic periodontitis, an inflammatory disease of the tooth-supporting tissues, leading to tooth loss. fusobacterium nucleatum, an opportunistic pathogen, is thought to promote dental plaque formation by serving as a bridge bacterium between early- and late-colonizing species of the oral cavity. previous studies have shown that f. nucleatum species synergize with t. forsythia during biofilm formation and pathogenesis. in the present study, we show ... | 2012 | 22547549 |
| genome-wide association study of periodontal pathogen colonization. | pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. to date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. we conducted a genome-wide association (gwa) study among 1,020 white participants of the atherosclerosis risk in communities study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom "checkerboard" dna-dna hybridization quantificat ... | 2012 | 22699663 |
| treponema denticola superoxide reductase: in vivo role, in vitro reactivities, and a novel [fe(cys)(4)] site. | in vitro and in vivo results are presented demonstrating that superoxide reductase (sor) from the air-sensitive oral spirochete, treponema denticola (td), is a principal enzymatic scavenger of superoxide in this organism. this sor contains the characteristic non-heme [fe(his)(4)cys] active sites. no other metal-binding domain has been annotated for td sor. however, we found that td sor also accommodates a [fe(cys)(4)] site whose spectroscopic and redox properties resemble those in so-called 2fe- ... | 2012 | 22715932 |
| polymorphic toxin systems: comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomics. | proteinaceous toxins are observed across all levels of inter-organismal and intra-genomic conflicts. these include recently discovered prokaryotic polymorphic toxin systems implicated in intra-specific conflicts. they are characterized by a remarkable diversity of c-terminal toxin domains generated by recombination with standalone toxin-coding cassettes. prior analysis revealed a striking diversity of nuclease and deaminase domains among the toxin modules. we systematically investigated polymorp ... | 2012 | 22731697 |
| the effects of extracellular matrix proteins on neutrophil-endothelial interaction--a roadway to multiple therapeutic opportunities. | polymorphoneuclear leukocytes or neutrophils, a major component of white blood cells, contribute to the innate immune response in humans. upon sensing changes in the microenvironment, neutrophils adhere to the vascular wall, migrate through the endothelial cell (ec)-pericyte bilayer, and subsequently through the extracellular matrix to reach the site of inflammation. these cells are capable of destroying microbes, cell debris, and foreign proteins by oxidative and non-oxidative processes. while ... | 2012 | 22737047 |
| halitosis: the multidisciplinary approach. | halitosis, bad breath or oral malodour are all synonyms for the same pathology. halitosis has a large social and economic impact. for the majority of patients suffering from bad breath, it causes embarrassment and affects their social communication and life. moreover,halitosis can be indicative of underlying diseases. only a limited number of scientific publications were presented in this field until 1995. ever since, a large amount of research is published, often with lack of evidence. in gener ... | 2012 | 22722640 |
| multiplicity of salmonella entry mechanisms, a new paradigm for salmonella pathogenesis. | the salmonella enterica species includes about 2600 diverse serotypes, most of which cause a wide range of food- and water-borne diseases ranging from self-limiting gastroenteritis to typhoid fever in both humans and animals. moreover, some serotypes are restricted to a few animal species, whereas other serotypes are able to infect plants as well as cold- and warm-blooded animals. an essential feature of the pathogenicity of salmonella is its capacity to cross a number of barriers requiring inva ... | 2012 | 23170225 |
| identification of a unique tlr2-interacting peptide motif in a microbial leucine-rich repeat protein. | pathogenesis of many bacterially-induced inflammatory diseases is driven by toll-like receptor (tlr) mediated immune responses following recognition of bacterial factors by different tlrs. periodontitis is a chronic inflammation of the tooth supporting apparatus often leading to tooth loss, and is caused by a gram-negative bacterial consortium that includes tannerella forsythia. this bacterium expresses a virulence factor, the bspa, which drives periodontal inflammation by activating tlr2. the n ... | 2012 | 22695115 |
| recent progress in synthetic biology for microbial production of c3-c10 alcohols. | the growing need to address current energy and environmental problems has sparked an interest in developing improved biological methods to produce liquid fuels from renewable sources. while microbial ethanol production is well established, higher-chain alcohols possess chemical properties that are more similar to gasoline. unfortunately, these alcohols (except 1-butanol) are not produced efficiently in natural microorganisms, and thus economical production in industrial volumes remains a challen ... | 2012 | 22701113 |
| occurrence of periodontal pathogens among patients with chronic periodontitis. | the aim of the present study was to evaluate the presence of the periodontal pathogens that form the red complex (tannerella forsythia, porphyromonas gingivalis and treponema denticola) and aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. the sample consisted of 29 patients with a clinical and radiographic diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology (3). samples for microbiological analysis were collected from th ... | 2012 | 24031906 |
| insights into the virulence of oral biofilms: discoveries from proteomics. | this review covers developments in the study of polymicrobial communities, biofilms and selected areas of host response relevant to dental plaque and related areas of oral biology. the emphasis is on recent studies in which proteomic methods, particularly those using mass spectrometry as a readout, have played a major role in the investigation. the last 5-10 years have seen a transition of such methods from the periphery of oral biology to the mainstream, as in other areas of biomedical science. ... | 2012 | 22809209 |
| co-localized or randomly distributed? pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis. | the polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. a number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. to examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analy ... | 2012 | 22655057 |
| causes of adverse pregnancy outcomes and the role of maternal periodontal status - a review of the literature. | preterm (pt) and low birth weight (lbw) are considered to be the most relevant biological determinants of newborn infants survival, both in developed and in developing countries. numerous risk factors for pt and lbw have been defined in the literature. infections of the genitourinary tract infections along with various biological and genetic factors are considered to be the most common etiological factors for pt/lbw deliveries. however, evidence suggests that sub-clinical infection sites that ar ... | 2012 | 22615718 |
| relationship between oral anaerobic bacteria and otitis media with effusion. | in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome. | 2012 | 22606045 |
| spontaneous periodontitis development in diabetic rats involves an unrestricted expression of inflammatory cytokines and tissue destructive factors in the absence of major changes in commensal oral microbiota. | diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia is a main feature. the objective was to evaluate the involvement of rage, inflammatory cytokines, and metalloproteinases in spontaneous periodontitis triggered by diabetes induction. immunohistochemical procedures for mmp-2, mmp-9, tnf-α, il-1β, il-6, rankl, and rage were performed in rats after 1, 3, 6, 9, and 12 months of diabetes induction. total dna was extracted from paraffin-embedded tissues and evaluated by re ... | 2012 | 22611374 |
| identification of an intracellular m17 family leucine aminopeptidase that is required for virulence in staphylococcus aureus. | staphylococcus aureus is a highly virulent bacterial pathogen capable of causing a variety of ailments throughout the human body. it is a major public health concern due to the continued emergence of highly pathogenic methicillin resistant strains (mrsa) both within hospitals and in the community. virulence in s. aureus is mediated by an array of secreted and cell wall associated virulence factors, including toxins, hemolysins and proteases. in this work we identify a leucine aminopeptidase (lap ... | 2012 | 22613209 |
| serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease. | chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects. | 2012 | 22546352 |
| prevalence of fima genotypes of porphyromonas gingivalis and other periodontal bacteria in a spanish population with chronic periodontitis. | the aim of this study was to determine the prevalence of the different fima genotypes of porphyromonas gingivalis in adult spanish patients with chronic periodontitis, patients with gingivitis and periodontally healthy subjects, and the relationship between these genotypes and other periodontopathogenic bacteria. | 2012 | 22549664 |
| tprc/d (tp0117/131), a trimeric, pore-forming rare outer membrane protein of treponema pallidum, has a bipartite domain structure. | identification of treponema pallidum rare outer membrane proteins (omps) has been a longstanding objective of syphilis researchers. we recently developed a consensus computational framework that employs a battery of cellular localization and topological prediction tools to generate ranked clusters of candidate rare omps (d. l. cox et al., infect. immun. 78:5178-5194, 2010). tp0117/tp0131 (tprc/d), a member of the t. pallidum repeat (tpr) family, was a highly ranked candidate. circular dichroism, ... | 2012 | 22389487 |
| oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. | oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients. | 2012 | 22531876 |