Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| tel1, a gene involved in controlling telomere length in s. cerevisiae, is homologous to the human ataxia telangiectasia gene. | yeast chromosomes terminate in tracts of simple repetitive dna (poly[g1-3t]). mutations in the gene tel1 result in shortened telomeres. sequence analysis of tel1 indicates that it encodes a very large (322 kda) protein with amino acid motifs found in phosphatidylinositol/protein kinases. the closest homolog to tel1 is the human ataxia telangiectasia gene. | 1995 | 7671310 |
| cloning and expression of human deoxyhypusine synthase cdna. structure-function studies with the recombinant enzyme and mutant proteins. | deoxyhypusine synthase catalyzes the first step in the post-translational formation of hypusine (n epsilon-(4-amino-2-hydroxybutyl)lysine). cdna clones encoding deoxyhypusine synthase were isolated from a human hela cell library. full-length cdna clones encoding a 369-amino acid protein (calculated molecular mass of 40,970 da) and a shorter cdna clone that would potentially encode a protein with an internal deletion of 56 amino acids (asp262-ser317) were isolated. the deduced amino acid sequence ... | 1995 | 7673224 |
| structure-function relationship of the eukaryotic dna replication factor, proliferating cell nuclear antigen. | proliferating cell nuclear antigen (pcna) is essential for eukaryotic dna replication and functions as a processivity factor of dna polymerase delta (pol delta). due to the functional and structural similarity with the beta-subunit of escherichia coli dna polymerase iii, it has been proposed that pcna would act as a molecular clamp during dna synthesis. by site-directed mutagenesis and biochemical analyses, we have studied the functional domains of human pcna required for stimulation of replicat ... | 1995 | 7673244 |
| suppression of the yeast mutation rft1-1 by human p53. | mutations in the gene encoding p53 have been found to be the most common genetic alterations in human cancer, p53 is thought to exert its function of tumor suppression through inhibition of cell proliferation or induction of apoptosis in response to dna damage. although there have been no proteins homologous to p53 identified in lower eucaryotic organisms, it is known that overexpression of wild-type human p53 can inhibit cell growth of schizosaccharomyces pombe and saccharomyces cerevisiae (bis ... | 1995 | 7673248 |
| identification of guanine exchange factor key residues involved in exchange activity and ras interaction. | we have carried out a functional analysis of the human hgrf55 exchange factor in the yeast saccharomyces cerevisiae. twelve residues conserved among most of all known guanine exchange factors (gefs) have been independently changed to alanine. taking advantage of the ability of hgrf55p to replace the yeast cdc25p exchange factor, and using the two-hybrid system with ras2ala22 allele, we have identified key residues for the interaction with ras and/or its activation. substitution of arginine 392 t ... | 1995 | 7675454 |
| cytogenic and molecular analysis of an aggressive angiomyxoma. | aggressive angiomyxoma is a rare mesenchymal tumor occurring mainly in the vulvar region extending into the paravaginal and perirectal region. histologically, this tumor is rich in vascular structures and in collagen fibers and is of myxoid appearance. cytogenetic and molecular analysis was performed on a case of an aggressive angiomyxoma and revealed clonal karyotypic abnormalities. all 50 metaphases analyzed showed a translocation involving the chromosomal region 12q14-15. chromosomal aberrati ... | 1995 | 7677171 |
| cyclin ubiquitination: the destructive end of mitosis. | 1995 | 7736567 | |
| cdc27hs colocalizes with cdc16hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. | we have isolated cdnas and raised antibodies corresponding to the human homologs of the s. cerevisiae cdc27 and cdc16 proteins, which are tetratrico peptide repeat (tpr)-containing proteins essential for mitosis in budding yeast. we find that the cdc27hs and cdc16hs proteins colocalize to the centrosome at all stages of the mammalian cell cycle, and to the mitotic spindle. injection of affinity-purified anti-cdc27hs antibodies into logarithmically growing hela cells causes a highly reproducible ... | 1995 | 7736578 |
| subregional localization of 14 yeast artificial chromosomes to human chromosome region 1p by fluorescence in situ hybridization. | we have sublocalized to the region between 1p22 and 1p33 a total of 14 yeast artificial chromosomes previously assigned to a broader area of human chromosome 1p. our purpose was to map dna sequences that could be used for the molecular characterization of the two common fragile sites present in bands 1p31.2 and 1p32, the expression of which is increased in patients with neuroblastomas. | 1995 | 7736782 |
| the yeast spt14 gene is homologous to the human pig-a gene and is required for gpi anchor synthesis. | the protein encoded by the yeast gene spt14 shows high sequence similarity to the human protein, pig-a, whose loss of activity is at the origin of the disease paroxysmal nocturnal hemoglobinuria. the symptoms of this disease are apparently due to a loss of cell surface, glycosylphosphatidylinositol (gpi)-anchored proteins. like pig-a mutant cells, spt14 mutant cells are defective in gpi anchoring due to a defect in the synthesis of glcnac-pi, the first step of gpi synthesis. the spt14 mutant cau ... | 1995 | 7737116 |
| the presence of trna pseudogenes in mammalia and plants and their absence in yeast may account for different specificities of pre-trna processing enzymes. | six of 13 cloned members of the human trna(val) gene family code for trna(val) pseudogenes, of which all but one are transcribed efficiently in hela cell extracts. due to single or multiple mismatches in stem regions, the corresponding pre-trnas are resistant against the action of human 5'- and 3'-processing enzymes and are thus prevented from being converted to mature trnas. surprisingly, all of them are accurately and efficiently processed to mature-sized trna in yeast nuclear extract. this is ... | 1995 | 7737506 |
| scaled-up expression of human alpha 2,6(n)sialyltransferase in saccharomyces cerevisiae. | expression of recombinant full length human alpha 2,6(n)sialyltransferase has been scaled-up in s. cerevisiae in a 150-l bioreactor yielding 47 u at a concentration of 0.31 u/l. the protein specific activity as measured in reconstituted yeast lyophilisate was 0.8 mu/mg protein. the recombinant enzyme exhibited similar michaelis constants as previously determined for the native rat enzyme. by immunoblotting the enzyme was shown to be heterogeneous by size (44-48 kd) and n-glycosylated. we conclud ... | 1995 | 7741734 |
| sugar specificity of human beta-cell glucokinase: correlation of molecular models with kinetic measurements. | human beta-cell glucokinase recognition and phosphorylation of different sugars was investigated by steady-state kinetic analysis, measurements of substrate-induced intrinsic fluorescence changes, and molecular modeling and calculation of interaction energies. measurements of kcat/km showed that glucokinase phosphorylated the sugars in the order glucose = mannose > deoxyglucose > fructose = glucosamine. the mode of binding of these sugars to the open conformation of glucokinase was predicted fro ... | 1995 | 7742312 |
| regulation of the human wee1hu cdk tyrosine 15-kinase during the cell cycle. | in higher eukaryotes, the cyclin-dependent kinases (cdks) are negatively regulated by phosphorylation on threonine 14 (t14) and tyrosine 15 (y15). in fission yeast, the wee1 and mitosis inhibitory kinase 1 (mik1) protein kinases phosphorylate y15 in cdc2. wee1hu is the only known protein kinase that can carry out this inhibitory phosphorylation on y15 in higher eukaryotes. in the present study, we examined the endogenous products of wee1hu in human cells and found that the original wee1hu cdna l ... | 1995 | 7743995 |
| establishment of the marker order pter-nras-ngfb-d1s189-d1s252-d1s440-d1s453-d1s514-cen-d1s442-d1s498-qte r in relation to the centromere on human chromosome 1. | recent developments in genetic linkage mapping of the human genome have generated a large number of short tandem repeat polymorphic markers (weissenbach et al. 1992, gyapay et al. 1994), and eventual integration of these markers into a physical map is a logical progression. a number of généthon microsatellite (ca repeat) markers have been provisionally localized to 1p13, but their exact position with respect to other sequences is unknown. in order to confirm the order of these markers and their ... | 1995 | 7749565 |
| detection of streptococcus pneumoniae in whole blood by pcr. | streptococcus pneumoniae is a major cause of bacteremia in both children and adults. currently, the diagnosis of pneumococcal bacteremia relies on the isolation and identification of the bacteria from blood cultures. we have developed a sensitive assay for the detection of s. pneumoniae in whole blood by the pcr. a specific primer-probe set (jm201 and jm202 primers with jm204 probe) designed from the penicillin-binding protein 2b gene was demonstrated to reproducibly detect between 10 and 100 fg ... | 1995 | 7751363 |
| cdna cloning of a new putative atpase subunit p45 of the human 26s proteasome, a homolog of yeast transcriptional factor sug1p. | the nucleotide sequence of a cdna that encodes a new regulatory subunit, named p45, of the 26s proteasome of human hepatoblastoma hepg2 cells has been determined. the polypeptide predicted from the open reading frame consists of 406 amino acid residues with a calculated molecular weight of 45770 and isoelectric point of 8.35. the sequences of several fragments of bovine p45, determined by protein chemical analyses, spanning 27% of the complete structure, were found to be in excellent accord with ... | 1995 | 7729537 |
| localization of i2-imidazoline binding sites on monoamine oxidases. | imidazoline binding sites (ibs) were proposed to be responsible for some of the pharmacological and therapeutic activities of imidazoline and related compounds and have been classified into two subtypes, i1bs and i2bs. convergent studies attribute a role in central blood pressure regulation to the i1bs. in contrast, the function of i2bs remains unknown. in the present study, by combining biochemical and molecular biology approaches, we show that 1) microsequencing of i2bs purified from rabbit ki ... | 1995 | 7730367 |
| immunotechnological trends in radioimmunotargeting: from 'magic bullet' to 'smart bomb'. | the impact of recent advances in the chemical and genetic engineering manipulations of antibodies on radioimmunotargeting is reviewed both in relation to radioimmunoscintigraphy and radioimmunotherapy. the resulting trends are: (1) the linking of parts of the mouse/rat and human antibody molecule; (2) the creation of molecules with dual antigen or multiple antigen recognition capabilities; (3) the making of smaller and smaller antigen recognition molecules; and (4) the development of molecules w ... | 1995 | 7731620 |
| a simple p53 functional assay for screening cell lines, blood, and tumors. | mutations in the p53 gene are implicated in the pathogenesis of half of all human tumors. we have developed a simple functional assay for p53 mutation in which human p53 expressed in saccharomyces cerevisiae activates transcription of the ade2 gene. consequently, yeast colonies containing wild-type p53 are white and colonies containing mutant p53 are red. since this assay tests the critical biological function of p53, it can distinguish inactivating mutations from functionally silent mutations. ... | 1995 | 7732013 |
| basal promoter elements as a selective determinant of transcriptional activator function. | in eukaryotes, activation of transcription involves an interplay between activators bound to cis-regulatory elements and factors bound to basal elements near the start site of transcription. the basal elements, for example the tata box or proximal sequence element (pse) of small nuclear rna (snrna) promoters, nucleate the assembly of basal transcription complexes, components of which interact with activators. although one basal transcription complex can interact with many activators, it is uncle ... | 1995 | 7715708 |
| identification of a dual specificity kinase that activates the jun kinases and p38-mpk2. | one ras-dependent protein kinase cascade leading from growth factor receptors to the erk (extracellular signal-regulated kinases) subgroup of mitogen-activated protein kinases (mapks) is dependent on the protein kinase raf-1, which activates the mek (mapk or erk kinase) dual specificity kinases. a second protein kinase cascade leading to activation of the jun kinases (jnks) is dependent on mekk (mek kinase). a dual-specificity kinase that activates jnk, named jnkk, was identified that functions ... | 1995 | 7716521 |
| intracellular pattern of cytosolic ca2+ changes during adhesion and multiple phagocytosis in human neutrophils. dynamics of intracellular ca2+ stores. | the subcellular pattern of cytosolic free ca2+ ([ca2+]i) changes in human polymorphonuclear neutrophils (pmns) was studied using imaging of fura-2 fluorescence (time resolution 12.5 ratios/s) to determine whether pmns could obtain directional information from the [ca2+]i signal. [ca2+]i changes were observed during initial adherence, the subsequent chemotactic movement, and the phagocytosis of opsonized yeast particles. initial adherence was followed by a rapid increase in [ca2+]i (from 90 +/- 1 ... | 1995 | 7718891 |
| high-level production of a human membrane protein in yeast: the peripheral-type benzodiazepine receptor. | the human peripheral-type benzodiazepine receptor (pbr) has been produced in saccharomyces cerevisiae where it retains its pharmacological properties [riond et al., eur. j. pharmacol. 208 (1991) 307-312]. as the rate of production was low, we analysed the mrna level, the effect of variation of the 5' sequence and the production in mitochondria. translation rather than transcription or targeting was found to be the main limiting factor. we were able to produce a chimeric pbr, with an n-terminal e ... | 1995 | 7721090 |
| characterization of the amino-terminal transcriptional activation function of the human estrogen receptor in animal and yeast cells. | we have previously reported that the transcriptional activation function af-1, located in the a/b region of the human estrogen receptor, exhibits cell-type and promoter context specificity in both animal cells and yeast. to further characterize af-1, we have constructed a number of deletion mutants spanning the a/b region in the context of either the whole human estrogen receptor or the a/b region linked to the gal4 dna binding domain, and tested their transcriptional activity in chicken embryo ... | 1995 | 7721882 |
| identification of the gene (ssu71/tfg1) encoding the largest subunit of transcription factor tfiif as a suppressor of a tfiib mutation in saccharomyces cerevisiae. | mutations in the saccharomyces cerevisiae ssu71 gene were isolated as suppressors of a transcription factor tfiib defect that confers both a cold-sensitive growth defect and a downstream shift in transcription start-site selection at the cyc1 locus. the ssu71-1 suppressor not only suppresses the conditional phenotype but also restores the normal pattern of transcription initiation at cyc1. in addition, the ssu71-1 suppressor confers a heat-sensitive phenotype that is dependent upon the presence ... | 1995 | 7724527 |
| oxidation of benzo[a]pyrene by recombinant human cytochrome p450 enzymes. | the oxidation of benzo[a]pyrene (b[a]p) was examined using reconstituted systems prepared with recombinant human cytochrome p450 (p450) enzymes 1a1, 1a2, 2c8, 2c10, 2e1, and 3a4 and with microsomes prepared from saccharomyces cerevisiae expressing recombinant human p450s 2c8, 2c9, and 2c18. products measured by hplc included the 3- and 9-phenols, the 4,5-, 7,8-, and 9,10-dihydrodiols (detected in the presence of epoxide hydrolase), and products in the polar fraction eluting immediately after the ... | 1995 | 7703357 |
| interaction of the cooh-terminal transactivation domain of p65 nf-kappa b with tata-binding protein, transcription factor iib, and coactivators. | we show that the transactivating cooh terminus of the p65 subunit of human transcription factor nf-kappa b directly binds the general transcription factors tfiib and tata-binding protein (tbp) in vitro. interaction of p65 with tfiib required the most cooh-terminal sequence repeat within tfiib. a functional interaction of tfiib with p65 was evident from assays in yeast cells. cotransfection experiments in cos cells revealed that only overexpression of tbp was able to further stimulate p65-depende ... | 1995 | 7706261 |
| various candida and torulopsis species differ in their ability to induce the production of c3, factor b and granulocyte-macrophage colony-stimulating factor (gm-csf) in human monocyte cultures. | the incidence of infections with candida albicans and also with non-albicans yeast species is increasing rapidly, particularly in immunocompromised patients. eight candida and torulopsis species were compared for their ability to stimulate production of complement components c3 and factor b by monocytes. in addition, the release of granulocyte-macrophage colony-stimulating factor (gm-csf) was determined, because this cytokine affects monocyte complement production. the highest ranked pathogenic ... | 1995 | 7707338 |
| aceruloplasminemia: molecular characterization of this disorder of iron metabolism. | ceruloplasmin is an abundant alpha 2-serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species. we report here on the identification of a genetic defect in the ceruloplasmin gene in a patient previously noted to have a total absence of circulating serum ceruloplasmin in association with late-onset retinal and basal ganglia degeneration. in this patient t2 (transverse relaxation time)-weighted magnetic resonance imaging of the brain revealed basal ganglia densit ... | 1995 | 7708681 |
| a domain of human immunodeficiency virus type 1 vpr containing repeated h(s/f)rig amino acid motifs causes cell growth arrest and structural defects. | vpr is a virion-associated protein of human immunodeficiency type 1 (hiv-1) whose function in acquired immunodeficiency syndrome (aids) has been uncertain. employing the yeast saccharomyces cerevisiae as a model to examine the effects of hiv-1 auxiliary proteins on basic cellular functions, we found that the vpr gene caused cell growth arrest and structural defects indicated by osmotic sensitivity and gross cell enlargement. production of various domains by gene expression showed that this effec ... | 1995 | 7708721 |
| diverse incidences of individual oligopeptides (dipeptidic to hexapeptidic) in proteins of human, bakers' yeast, and escherichia coli origin registered in the swiss-prot data base. | oligopeptidic permutations of the 20 amino acid residues give rise to proteins of diverse functions. our long-term goal is to produce a lexicon of oligopeptides, classifying them into at least five categories: (i) ubiquitous, (ii) function specific, (iii) group specific, (iv) species specific, and (v) nonexistent. to begin with, we report on the varying frequencies of individual oligopeptides (dipeptidic to hexapeptidic in length) found among 2862 human proteins, 1942 saccharomyces cerevisiae pr ... | 1995 | 7708741 |
| functional expression of fused enzymes between human cytochrome p4501a1 and human nadph-cytochrome p450 oxidoreductase in saccharomyces cerevisiae. | the activity of human cytochrome p450 enzymes heterologously expressed in saccaromyces cerevisiae cells is limited by the yeast endogenous cytochrome p450 oxidoreductase (yor). to overcome these limitations, we constructed hybrids between human p4501a1 (cyp1a1) and human p450 oxidoreductase (hor) by combining the cdna encoding hor with the cyp1a1 cdna. in addition, in one construct, the amino terminus of hor was replaced by the membrane anchor domain of a yeast protein. anchoring of the fusion c ... | 1995 | 7710684 |
| the shortest isoform of human vascular endothelial growth factor/vascular permeability factor (vegf/vpf121) produced by saccharomyces cerevisiae promotes both angiogenesis and vascular permeability. | vascular endothelial growth factor/vascular permeability factor (vegf/vpf) is a multifunctional cytokine that is expressed as four isoforms having 206, 189, 165, and 121 amino acids in humans. we constructed a system that produces the shortest isoform of human vegf/vpf in saccharomyces cerevisiae (yvegf/vpf121). active yvegf/vpf121 was secreted from the yeast cells as a glycosylated dimeric protein. various biological activities of the purified yvegf/vpf121 were examined. it bound to cell surfac ... | 1995 | 7533000 |
| distinguishing ingested ethanol from microbial formation by analysis of urinary 5-hydroxytryptophol and 5-hydroxyindoleacetic acid. | during the metabolism of ethanol, the metabolic conversion of serotonin (5-hydroxytryptamine) is altered, and, as a consequence, the ratio of 5-hydroxytryptophol (5htol) to 5-hydroxyindole-3-acetic acid (5hiaa) excreted in urine increases appreciably. the ratio of metabolites remains elevated for several hours after ethanol is no longer detectable. in the present study, urine specimens were supplemented with glucose and candida albicans, a common human pathogenic yeast, and the formation of etha ... | 1995 | 7533206 |
| production of pigs transgenic for human regulators of complement activation. | 1995 | 7533399 | |
| the growth-inhibitory block of tgf-beta is located close to the g1/s border in the cell cycle. | transforming growth factor-beta (tgf-beta) inhibits dna synthesis in dense cultures of young human embryonic fibroblasts and antagonizes the mitogenic action of platelet-derived growth factor (pdgf). the inhibition of the pdgf-bb action by tgf-beta was independent of the induction of mrnas for the pdgf-a chain and pdgf-beta receptor, the predominant types of pdgf receptor in human fibroblasts. the tgf-beta-mediated inhibition did not influence the expression of various genes that are involved in ... | 1995 | 7698248 |
| expression in escherichia coli, purification and functional activity of recombinant human chaperonin 10. | we have recently reported the cloning of a cdna coding for a stress inducible human chaperonin 10. the protein was shown to possess 100% identity with the bovine homologue and a single amino acid replacement (glycine to serine at position 52) compared to rat chaperonin 10. here we report the heterologous expression of human chaperonin 10 in escherichia coli, its purification and its functional characterization. the recombinant protein was purified to homogeneity as judged by different analytical ... | 1995 | 7698325 |
| isolation and sequencing of two cdna clones encoding rho proteins from the fission yeast schizosaccharomyces pombe. | the rho genes encode a group of low-molecular-weight gtp-binding proteins that show about 30% identity in the amino-acid sequence to the ras gene product. two cdna clones, both of which are similar to the rho genes, were isolated from a cdna library of the fission yeast schizosaccharomyces pombe, using the human rhoa cdna as a probe. these genes were called rho1+ and rho2+. the encoded rho1 protein showed 72.7% identity to the budding yeast rho1 and 66.8% to human rhoa, and the encoded rho2 prot ... | 1995 | 7698654 |
| identification of the chromosome 12 translocation breakpoint region of a pleomorphic salivary gland adenoma with t(1;12)(p22;q15) as the sole cytogenetic abnormality. | cell line ad-312/sv40, which was derived from a primary pleomorphic salivary gland adenoma with t(1;12)(p22;q15), was used in fluorescence in situ hybridization (fish) analysis to characterize its translocation breakpoint region on chromosome 12. results of previous studies have indicated that the chromosome 12 breakpoint in ad-312/sv40 is located proximally to locus d12s8 and distally to the chop gene. we here describe two partially overlapping yeast artificial chromosome (yac) clones, y4854 (5 ... | 1995 | 7850744 |
| properties of the catalytic domain of sdc25p, a yeast gdp/gtp exchange factor of ras proteins. complexation with wild-type ras2p, [s24n]ras2p and [r80d, n81d]ras2p. | the catalytic domain of the saccharomyces cerevisiae sdc25 gene product, including the last 550 c-terminal residues (sdc25p-c), was produced as an escherichia coli recombinant protein fused with glutathione s-transferase. the highly purified (greater than 95%) stable fusion protein, obtained by affinity chromatography, was very active in enhancing the dissociation rate or the gdp/gtp exchange of the gdp complex of ras2p or human h-ras p21. this activity was further increased (three times) by glu ... | 1995 | 7851434 |
| isolation and mapping of a human gene (mcm2) encoding a product homologous to yeast proteins involved in dna replication. | from a human fetal-lung cdna library, we isolated a gene, human mcm2, whose nucleotide sequence predicts a protein product homologous to the yeast nuclear proteins mcm2, mcm3, cdc21, and cdc46. determination of cdna sequences covering the entire coding region demonstrated an open reading frame of 1,629 nucleotides encoding 543 amino acids. sequence comparison revealed 30-40% amino acid identity with these four yeast proteins, all of which are considered to play important roles in dna replication ... | 1995 | 7842741 |
| molecular cloning and functional expression in yeast of a human camp-specific phosphodiesterase subtype (pde iv-c). | we have recently reported increased survival of dopaminergic substantia nigra neurons by inhibition of phosphodiesterase type iv enzymes. as a first step to unravel the involvement of pde iv subtypes in this process, we isolated phosphodiesterase type iv cdnas from human substantia nigra. one isolated partial cdna clone was most homologous to the partially cloned rat and human pde iv-c isogene. distribution analysis revealed that the enzyme is expressed in various tissues but not in cells of the ... | 1995 | 7843419 |
| evaluation of the sa locus in human hypertension. | the sa gene is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat compared with the normotensive wistar-kyoto rat. the gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. to assess its role in human hypertension, a human sa cdna was cloned from a liver library. the cdna was 1513 bp in length and exhibited a high identity with the published rat sa cdna s ... | 1995 | 7843754 |
| description of a 700-kb yeast artificial chromosome contig containing the bcl1 translocation breakpoint region at 11q13. | we screened two human yeast artificial chromosome (yac) libraries by polymerase chain reaction (pcr) with oligonucleotides specific to the bcl1 major translocation breakpoint cluster region at 11q13. five yacs were isolated. two of them were chimeric. one of these and remaining three yacs were characterized by hybridization with various known 11q13 probes, alu-pcr fingerprinting, in situ hybridization, and isolation of yac ends. a map of this ca 700-kb yac contig was obtained. this map was consi ... | 1995 | 7835075 |
| genes for sma: multum in parvo. | 1995 | 7813005 | |
| noninhibitory binding of human interleukin-2-activated natural killer cells to the germ tube forms of candida albicans. | during incubation in vitro with yeast or germ tube forms of candida albicans, only 2 to 6% of freshly isolated human natural killer (nk) cells (> 85% cd16+, cd56+, cd3-; < 15% cd3+; cytolytic for the nk-susceptible target k562 but not for the nk-resistant target daudi), were seen to interact with the fungal cells. as seen under the electron microscope, the contact area had a limited extent and was narrow, and neither the surface nor the intracytoplasmic organization of the nk cell was altered. i ... | 1995 | 7806367 |
| ataxia with vitamin e deficiency: refinement of genetic localization and analysis of linkage disequilibrium by using new markers in 14 families. | ataxia with vitamin e deficiency (aved) is an autosomal recessive disease characterized clinically by neurological symptoms with often striking resemblance to those of friedreich ataxia. this disorder has been reported previously as familial isolated vitamin e deficiency. we have mapped recently the aved locus to a 5-cm confidence interval on chromosome 8q by homozygosity mapping in six mediterranean families. we have now analyzed six new and two previously described families and demonstrate gen ... | 1995 | 7726167 |
| structure of the terminal 300 kb of dna from human chromosome 21q. | the most distal 300 kb of human chromosome 21q was cloned and mapped using telomeric yeast artificial chromosomes (yacs). the region contains low-copy subtelomeric repeats at the telomeric end, chromosome 21-specific sequences more centromerically, and the s100b gene at a distance of 100-140 kb from the chromosome terminus. reca-assisted restriction endonuclease cleavage of genomic dna showed that the cloned fragments correspond to telomere-terminal genomic dna, and restriction enzyme mapping of ... | 1995 | 7782083 |
| ttf, a gene encoding a novel small g protein, fuses to the lymphoma-associated laz3 gene by t(3;4) chromosomal translocation. | we have previously shown that the laz3/bcl6 gene encoding a potential transcription factor, is disrupted in b-diffuse large cell non-hodgkin's lymphomas (nhl) with 3q27 chromosomal abnormalities involving the immunoglobulin (ig) genes. however, laz3 rearrangement also occurs in nhl bearing 3q27 translocations without involvement of the ig genes: for example the val cell line exhibits t(3;4)(q27;p11). in the present work we have used a rt-pcr method to detect and to sequence the laz3 mrna product ... | 1995 | 7784061 |
| characterisation of the interaction between pcna and gadd45. | we have examined the interaction between the dna replication and repair protein pcna, and the growth arrest and dna damage induced protein gadd45. an anti-gadd45 polyclonal antibody co-immunoprecipitates pcna but in reciprocal experiments, an anti-c terminal anti-pcna antibody failed to co-immunoprecipitate gadd45. we used a yeast two hybrid assay to demonstrate that human gadd45 interacts with both human and s. pombe pcna. we have determined that the n-terminal 94 amino acids of gadd45 bind to ... | 1995 | 7784094 |
| transcriptional activation by the orphan nuclear receptor arp-1. | arp-1 is a ubiquitous orphan nuclear receptor that binds to a site (site a) in the apolipoprotein ai (apoai) liver-specific enhancer and represses its transcriptional activity in hepatoblastoma hepg2 cells. electrophoretic mobility shift analysis of hepg2 cell nuclear extracts showed that in addition to arp-1, site a also binds the orphan nuclear receptors ear-2 and hnf-4. in in vitro transcription assays, hela cell nuclear extracts which contain arp-1 had no effect on transcription from a basal ... | 1995 | 7784207 |
| assignment of the gene responsible for cystinuria (rbat) and of markers d2s119 and d2s177 to 2p16 by fluorescence in situ hybridization. | we have established rbat (named as slc3a1 in the genome data base) as a gene responsible for cystinuria, a heritable disorder of amino acid transport. the cystinuria locus has been mapped by linkage between microsatellite markers d2s119 and d2s177. fluorescence in situ hybridization (fish) either with alu-polymerase-chain-reaction (pcr)-amplified sequences of a yeast artificial chromosome (yac) containing the rbat gene or with rbat-specific pcr-amplified genomic fragments, and chromosome g-bandi ... | 1995 | 7789946 |
| oral candida: clearance, colonization, or candidiasis? | candida albicans is frequently isolated from the human mouth, yet few carriers develop clinical signs of candidiasis. oral candidiasis presents clinically in many forms. this reflects the ability of the yeast to colonize different oral surfaces and the variety of factors which predispose the host to candida colonization and subsequent infection. colonization of the oral cavity appears to be facilitated by several specific adherence interactions between c. albicans and oral surfaces which enable ... | 1995 | 7790592 |
| human type ii receptor for bone morphogenic proteins (bmps): extension of the two-kinase receptor model to the bmps. | bone morphogenic proteins (bmps) are universal regulators of animal development. we report the identification and cloning of the bmp type ii receptor (bmpr-ii), a missing component of this receptor system in vertebrates. bmpr-ii is a transmembrane serine/threonine kinase that binds bmp-2 and bmp-7 in association with multiple type i receptors, including bmpr-ia/brk1, bmpr-ib, and actr-i, which is also an activin type i receptor. cloning of bmpr-ii resulted from a strong interaction of its cytopl ... | 1995 | 7791754 |
| identification of the aminocatechol a-3253 as an in vitro poison of dna topoisomerase i from candida albicans. | the aminocatechol a-3253 is active against several pathogenic fungi, including candida albicans, cryptococcus albidus, and aspergillus niger. a-3253 interferes with both the in vitro biosynthesis of (1,3)-beta-glucan and the activity of topoisomerases i isolated from candida spp. it is likely that one or more of the enzymes involved in glucan biosynthesis rather than topoisomerase i is the primary intracellular target of a-3253, since a strain of saccharomyces cerevisiae lacking topoisomerase i ... | 1995 | 7793856 |
| aniridia-associated cytogenetic rearrangements suggest that a position effect may cause the mutant phenotype. | current evidence suggests that aniridia (absence of iris) is caused by loss of function of one copy of the pax6 gene, which maps to 11p13. we present the further characterisation of two aniridia pedigrees in which the disease segregates with chromosomal rearrangements which involve 11p13 but do not disrupt the pax6 gene. we have isolated three human yac clones which encompass the pax6 locus and we have used these to show that in both cases the chromosomal breakpoint is at least 85 kb distal of t ... | 1995 | 7795596 |
| evidence from antibody studies that the cag repeat in the huntington disease gene is expressed in the protein. | the neurodegenerative disorder huntington disease (hd) appears to be caused by an increase in the number of repeats of the trinucleotide cag located near the 5' end of the gene. the nucleotide sequences of the cdna and the gene predict that the hd protein has a molecular weight of 347,000 (3144 amino acids) and that the cag repeats encode a segment of polyglutamine beginning 17 amino acids from the amino terminus. because the cag repeat plays such a critical role in the etiology of the disease, ... | 1995 | 7795604 |
| the apolipoprotein(a) gene is regulated by sex hormones and acute-phase inducers in yac transgenic mice. | high plasma concentrations of apolipoprotein (a) (apo(a)) have been implicated as a major independent risk factor for atherosclerosis in humans. apo(a) is a large, evolutionarily new gene (present primarily in primates) for which considerable controversy exists concerning the factors that regulate its expression. to investigate the in vivo regulation of apo(a), we have created several lines of yac transgenic mice containing a 110-kb human apo(a) gene surrounded by greater than 60 kb of 5' and 3' ... | 1995 | 7795650 |
| identification of a major subfamily of large hsp70-like proteins through the cloning of the mammalian 110-kda heat shock protein. | a major mammalian heat shock protein of 110 kda (hsp110) has long been observed, but has not been cloned. we have cloned the hamster cdna for hsp110 and show that it hybridizes on a northern blot to a 3.5-kilobase heat-inducible message in hamster and mouse. the hsp110 sequence was found to share an approximately 30-33% amino acid identity with members of the hsp70 family, most of which occurs in the conserved atp-binding domain of these molecules. in addition, five sequences were found to be hi ... | 1995 | 7797574 |
| a yeast rna binding protein that resembles the human autoantigen la. | the la protein is a 47 kda polypeptide that frequently acts as an autoantigen in systemic lupus erythematosus and sjögren's syndrome patients. a key property of this protein is its association with the u-rich termini of newly synthesized rna polymerase iii transcripts. here we characterize a 32 kda protein from saccharomyces cerevisiae that shows sequence similarity to the n termini of vertebrate la proteins. this yeast protein also functionally resembles la in that it binds preferentially in vi ... | 1995 | 7799435 |
| preferential isolation of dna fragments associated with cpg islands. | we describe a procedure for preferential isolation of dna fragments with g+c-rich portions. such fragments occur in known genes within or adjacent to cpg islands. since about 56% of human genes are associated with cpg islands, isolation of these fragments permits detection and probing of many genes within much larger segments of dna, such as cosmids or yeast artificial chromosomes, which have not been sequenced. cloned dna fragments digested with four restriction endonucleases were subjected to ... | 1995 | 7753787 |
| heat shock protein hsp90 regulates dioxin receptor function in vivo. | the dioxin (aryl hydrocarbon) receptor is a ligand-dependent basic helix-loop-helix (bhlh) factor that binds to xenobiotic response elements of target promoters upon heterodimerization with the bhlh partner factor arnt. here we have replaced the bhlh motif of the dioxin receptor with a heterologous dna-binding domain to create fusion proteins that mediate ligand-dependent transcriptional enhancement in yeast (saccharomyces cerevisiae). previously, our experiments indicated that the ligand-free d ... | 1995 | 7753824 |
| interphase and metaphase detection of the breakpoint of 14q32 translocations in b-cell malignancies by double-color fluorescence in situ hybridization. | the breakpoint of 14q32 translocations found in b-cell malignancies was delineated specifically in both metaphase spreads and interphase nuclei by double-color fluorescence in situ hybridization (fish) using bacteriophage clones containing the human immunoglobulin gamma chain gene locus (ig gamma) and a cosmid clone, cy24-68, containing vh segments. cy24-68 is more telomeric than ig gamma, separated by approximately 1 megabase (mb). fish studies were performed on four patients with non-hodgkin's ... | 1995 | 7756653 |
| structure and function of asp, the human homolog of the mouse agouti gene. | the mouse agouti coat color gene encodes a novel paracrine signaling molecule whose pulsatile expression produces a characteristic pattern of banded pigment in individual hairs. several spontaneous agouti alleles produce adult-onset obesity and diabetes, and have provided important single-gene animal models for alterations in energy metabolism. utilizing linkage groups conserved between mice and humans, we have cloned the human homolog of the mouse agouti gene from a human chromosome 20 yeast ar ... | 1995 | 7757071 |
| genomic structure of human mismatch repair gene, hmlh1, and its mutation analysis in patients with hereditary non-polyposis colorectal cancer (hnpcc) | mutation of hmlh1, a gene involved in dna mismatch repair, is responsible for some families carrying the hereditary non-polypotic colorectal cancer (hnpcc) syndrome. to establish a basis for presymptomatic diagnosis of hnpcc patients who carry germline mutations in this gene, we determined the exon-intron organization of hmlh1. the results indicated that hmlh1 consists of 19 coding exons spanning approximately 100 kb, and that exons 1-7 contain a region that is highly conserved in the mlh1 and p ... | 1995 | 7757073 |
| isolation of a yac clone covering a cluster of nine s100 genes on human chromosome 1q21: rationale for a new nomenclature of the s100 calcium-binding protein family. | s100 proteins are low-molecular-weight calcium-binding proteins of the ef-hand superfamily and appear to be involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. more than 10 members of the s100 protein family have been described from human sources so far. we have now isolated a yac clone from human chromosome 1q21, on which 9 different genes coding for s100 calcium-binding proteins could be localized. moreover, we have mapped the gene c ... | 1995 | 7759097 |
| physical mapping of the human t-cell receptor beta gene complex, using yeast artificial chromosomes. | yeast artificial chromosomes (yacs) were used to construct a physical map of the germline human t-cell beta chain gene complex (tcrb). variable region genes (bv) for the 25 known subfamilies were used as probes to screen the icrf am4x yac library. of the five positive yacs identified, one yac designated b3, 820 kilobase pairs (kbp) in size, scored positive for all 25 tcrbv subfamilies plus the constant region genes (bc) when analyzed by pulse field gel electrophoresis. restriction enzyme mapping ... | 1995 | 7759129 |
| suppression of mutations in two saccharomyces cerevisiae genes by the adenovirus e1a protein. | the protein products of the adenoviral e1a gene are implicated in a variety of transcriptional and cell cycle events, involving interactions with several proteins present in human cells, including parts of the transcriptional machinery and negative regulators of cell division such as the rb gene product and p107. to determine if there are functional homologs of e1a in saccharomyces cerevisiae, we have developed a genetic screen for mutants that depend on e1a for growth. the screen is based on a ... | 1995 | 7760818 |
| isolation of proteins that interact specifically with the retinoid x receptor: two novel orphan receptors. | we have used a yeast genetic system to isolate cdnas encoding proteins that specifically interact with the ligand-binding domain of human retinoid x receptor-alpha (rxr alpha). a number encoded portions of two known rxr heterodimer partners, the retinoic acid receptor (rar) and the peroxisome proliferator activated receptor. of four additional rxr-interacting proteins (rips) selected for further study two, rip14 and rip15, are previously unidentified orphan members of the nuclear receptor superf ... | 1995 | 7760852 |
| mcm3 complex required for cell cycle regulation of dna replication in vertebrate cells. | an intact nuclear membrane restricts dna replication to only one round in each cell cycle, apparently by excluding an essential replication-licensing factor throughout interphase. a family of related yeast replication proteins, mcm2, 3 and 5 (also called, after cell-division cycle, cdc46), resemble licensing factor, entering the nucleus only during mitosis. we have cloned a xenopus homologue of mcm3 (xmcm3) and raised antibodies against expressed protein. immunodepletion of xenopus egg extracts ... | 1995 | 7760938 |
| wide cross-species aminoacyl-trna synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen. | because of variations in trna sequences in evolution, trna synthetases either do not acylate their cognate trnas from other organisms or execute misacylations which can be deleterious in vivo. we report here the cloning and primary sequence of a 958-aa saccharomyces cerevisiae alanyl-trna synthetase. the enzyme is a close homologue of the human and escherichia coli enzymes, particularly in the region of the primary structure needed for aminoacylation of rna duplex substrates based on alanine trn ... | 1995 | 7761427 |
| isolation and characterization of a mage gene family in the xp21.3 region. | a human gene with strong homology to the mage gene family located in xq27-qter has been isolated by using exon-trapping of cosmids in the xp21.3 region. we have mapped and sequenced cdna and genomic clones corresponding to this gene, mage-xp, and shown that the last exon contains the open reading frame and is present in a minimum of five copies in a 30-kb interval. mage-xp is expressed only in testis and, unlike the xq27-qter mage genes, it is not expressed in any of 12 different tumor tissues t ... | 1995 | 7761436 |
| molecular cloning and characterization of a conserved nuclear serine(threonine) protein kinase. | human, drosophila melanogaster, and caenorhabditis elegans cdna clones encoding homologues of a serine(threonine) protein kinase (ec 2.7.1.37) (designated ndr protein kinase) have been isolated and sequenced. the human and drosophila cdnas predict polypeptides of 54 kda and 52 kda, respectively, which share approximately 80% amino acid similarity. northern analysis of human tissues revealed a ubiquitously expressed 3.9-kb transcript. recombinant gst-ndr underwent intramolecular autophosphorylati ... | 1995 | 7761441 |
| nucleotide sequence and analysis of the centromeric region of yeast chromosome ix. | we have determined the nucleotide sequence of a cosmid (pix338) containing the centromere region of yeast (saccharomyces cerevisiae) chromosome ix. the complete nucleotide sequence of 33.8 kb was obtained by using an efficient directed sequencing strategy in combination with automated dna sequencing on the a.l.f. dna sequencer. sequence analysis revealed the presence of 17 open reading frames (orfs), four of them previously known yeast genes (sly12, pan1, sts1 and prl1), a trna gene and the cent ... | 1995 | 7762303 |
| identification of a yeast artificial chromosome clone spanning a translocation breakpoint at 7q32.1 in a smith-lemli-opitz syndrome patient. | smith-lemli-opitz syndrome (slos) is a mental retardation/multiple congenital anomaly syndrome. the gene(s) involved has not been mapped or cloned, but, recently, a biochemical abnormality in cholesterol biosynthesis has been shown to occur in most slos patients. the defect is suspected to occur in the penultimate step of the cholesterol pathway, involving the enzyme 7-dehydrocholesterol reductase, which has not been isolated. on the basis of the hypothesis that a de novo balanced translocation ... | 1995 | 7762564 |
| high level secretion by saccharomyces cerevisiae of human apolipoprotein e as a fusion to rhizomucor rennin. | as the first step for production of human apolipoprotein e (hapoe) in saccharomyces cerevisiae, the hapoe cdna was cloned in escherichia coli, on the basis of the nucleotide sequence reported previously. when the hapoe cdna including its pre-sequence-encoding region was expressed under the control of the gal7 promoter, no protein immunoreactive with anti-hapoe antibody was detected either in the culture medium or inside the cells. for efficient production and secretion of hapoe in s. cerevisiae, ... | 1995 | 7766173 |
| eea1, an early endosome-associated protein. eea1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding iq motif. | early endosomes are cellular compartments receiving endocytosed material and sorting them for vesicular transport to late endosomes and lysosomes or for recycling to the plasma membrane. we have cloned a human cdna encoding an evolutionarily conserved 180-kda protein on early endosomes named eea1 (early endosome antigen1). eea1 is associated with early endosomes since it co-localizes by immunofluorescence with the transferrin receptor and with rab5 but not with rab7. immunoelectron microscopy sh ... | 1995 | 7768953 |
| the swi-snf complex: a chromatin remodeling machine? | the swi-snf complex plays a key role in the regulation of eukaryotic gene expression. genetic studies in the yeast saccharomyces cerevisiae suggest that one role for the complex is to antagonize chromatin-mediated repression of transcription. recent biochemical studies indicate that s. cerevisiae and putative human swi-snf complexes use the energy of atp hydrolysis to disrupt nucleosome structure. | 1995 | 7770913 |
| a recombinant human hemoglobin with asparagine-102(beta) substituted by alanine has a limiting low oxygen affinity, reduced marginally by chloride. | a recombinant (r) mutant hemoglobin (hb) with asn-102(beta) replaced by an ala (n102a(beta)) has been prepared by pcr amplification of a mutagenic dna fragment and expression of the recombinant protein in yeast. the side chain of asn-102(beta) is part of an important region of the alpha 1 beta 2 interface that undergoes large structural changes in the transition between the deoxy and oxy conformations. three natural mutant hbs with neutral substitutions of thr, ser, or tyr at this site have low ... | 1995 | 7773172 |
| characterization of a 14q+ marker chromosome in philadelphia chromosome positive acute lymphoblastic leukemia by dna analysis and fluorescence in situ hybridization. | we report a case of precursor-b acute lymphoblastic leukemia (all) with the philadelphia chromosome (ph) and a 14q+ chromosome whose additional material was a part of the long arm of der(9)t(9;22). a minor population carrying the standard ph translocation without the 14q+ was also observed at the first presentation. the translocation of the bcr gene from chromosome 22 to the subtelomeric region of the 14q+ was confirmed by fluorescence in situ hybridization (fish) using a yeast artificial chromo ... | 1995 | 7773965 |
| construction of a transcription map surrounding the brca1 locus of human chromosome 17. | we have used a combination of methods (exon amplification, direct selection, direct screening, evolutionary conservation, island rescue-pcr, and direct sequence analysis) to survey approximately 600 kb of genomic dna surrounding the brca1 gene for transcribed sequences. we have cloned a set of fragments representing at least 26 genes. the dna sequence of these clones reveals that 5 are previously cloned genes; the precise chromosomal location of 2 was previously unknown, and 3 have been cloned a ... | 1995 | 7774924 |
| 22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors. | in our effort to identify brca1, 22 genes were cloned from a 1-mb region of chromosome 17q21 defined by meiotic recombinants in families with inherited breast and/or ovarian cancer. subsequent discovery of another meiotic recombinant narrowed the region to approximately 650 kb. genes were cloned from fibroblast and ovarian cdna libraries by direct screening with yacs and cosmids. the more than 400 cdna clones so identified were mapped to cosmids, yacs, and p1 clones and to a chromosome 17 somati ... | 1995 | 7774926 |
| a yac-, p1-, and cosmid-based physical map of the brca1 region on chromosome 17q21. | a familial early-onset breast cancer gene (brca1) has been localized to chromosome 17q21. to characterize this region and to aid in the identification of the brca1 gene, a physical map of a region of 1.0-1.5 mb between the edh17b1 and the ppy loci on chromosome 17q21 was generated. the physical map is composed of a yeast artificial chromosome (yac) and p1 phage contig with one gap. the majority of the interval has also been converted to a cosmid contig. twenty-three pcr-based sequence-tagged sit ... | 1995 | 7774927 |
| assignment of the 36.5-kda (rfc5), 37-kda (rfc4), 38-kda (rfc3), and 40-kda (rfc2) subunit genes of human replication factor c to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23. | replication factor c is a multimeric primer-recognition protein consisting of five subunits (p145, p40, p38, p37, and p36.5) and is essential for the processive elongation of dna chains catalyzed by dna polymerase delta or epsilon in human cells. we have mapped the locations on human chromosomes of the genes coding for the four smaller subunits [p36.5 (rfc5), p37 (rfc4), p38 (rfc3), and p40 (rfc2)] using both pcr amplification from dnas of a panel of somatic hybrids and fluorescence in situ hybr ... | 1995 | 7774928 |
| physical analysis of the tuberous sclerosis region in 9q34. | we report the construction of a physical map based on cloned dna within the candidate region for the tuberous sclerosis complex (tsc1) gene on chromosome 9q34, between the markers d9s149 and d9s66. the dna clones form three contigs consisting of 7 yacs, bridged by p1 and cosmid clones, and cover more than 950 kb of 9q34. despite intensive screening of all available libraries, two gaps remain. a detailed physical map of much of this region was derived, and restriction mapping of the yac, p1, and ... | 1995 | 7774937 |
| addition of a 29 residue carboxyl-terminal tail converts a simple hmg box-containing protein into a transcriptional activator. | human mitochondrial transcription factor a (h-mttfa) is essential for initiation of transcription from the two promoters located in the displacement-loop region of human mitochondrial dna. this 25 kda protein contains two tandem, hmg box dna-binding domains separated by a 27 amino acid residue linker region and followed by a 25 residue carboxyl-terminal tail; both the linker and tail are rich in basic amino acid residues. mutational analysis of h-mttfa revealed that the tail region is important ... | 1995 | 7776365 |
| sequence determination of human papillomavirus type 6a and assembly of virus-like particles in saccharomyces cerevisiae. | human papillomavirus 6a (hpv6a), the most abundant hpv6 subtype, was detected in a vulvar condyloma acuminatum. the complete genome of hpv6a was cloned, and its dna sequence was shown to be over 97% identical to the hpv6b sequence. of the eight open reading frames (orfs) of hpv6a, only the imputed amino acid sequence of the major capsid protein l1 was identical to the corresponding hpv6b sequence; all other hpv6a orfs showed amino acid changes compared to the hpv6b orfs. the hpv6a l1 or the l1 + ... | 1995 | 7778283 |
| a b-cell coactivator of octamer-binding transcription factors. | the octamer motif (atgcaaat) paradoxically plays a central role in mediating the activity of both b-cell specific and ubiquitous promoters. it has been widely assumed that the predominantly lymphoid-restricted octamer-binding factor oct-2 mediates tissue-specific promoter activity, whereas the ubiquitously expressed oct-1 mediates general promoter activity, but this view has been challenged. here we use a modified yeast one-hybrid assay to isolate a b-cell factor, bob1, which associates with eit ... | 1995 | 7779176 |
| delineating minimal protein domains and promoter elements for transcriptional activation by lentivirus tat proteins. | lentivirus tat proteins comprise a novel class of rna-binding transcriptional activators that are essential for viral replication. in this study, we performed a series of protein fusion experiments to delineate the minimal protein domains and promoter elements required for tat action. we show that a 15-amino-acid region of equine infectious anemia virus (eiav) tat protein, when fused to the gal4 or lexa dna binding domain, can activate transcription in appropriate promoter contexts. in the natur ... | 1995 | 7884911 |
| human alpha-tocopherol transfer protein: cdna cloning, expression and chromosomal localization. | alpha-tocopherol transfer protein (alpha ttp), which specifically binds this vitamin and enhances its transfer between separate membranes, was previously isolated from rat liver cytosol. in the current study we demonstrated the presence of alpha ttp in human liver by isolating its cdna from a human liver cdna library. the cdna for human alpha ttp predicts 278 amino acids with a calculated molecular mass of 31,749, and the sequence exhibits 94% similarity with rat alpha ttp at the amino acid leve ... | 1995 | 7887897 |
| mepyramine, a histamine h1 receptor antagonist, inhibits the metabolic activity of rat and human p450 2d forms. | the interaction of antihistaminics, including mepyramine, with rat hepatic cytochrome p450s (p450s) was investigated. we first investigated mepyramine binding to eight forms of rat hepatic p450s. mepyramine bound specifically to p450 2d1, which suggests that it inhibits p450 2d activity. histamine h1 receptor antagonists (mepyramine, diphenhydramine, chlorpheniramine and triprolidine) inhibited the lidocaine 3-hydroxylation activity catalyzed by p450 2d1 but did not inhibit the testosterone hydr ... | 1995 | 7853212 |
| a multigene family related to chitin synthase genes of yeast in the opportunistic pathogen aspergillus fumigatus. | two approaches were used to isolate fragments of chitin synthase genes from the opportunistic human pathogen aspergillus fumigatus. firstly, regions of amino acid conservation in chitin synthases of saccharomyces cerevisiae were used to design degenerate primers for amplification of portions of related genes, and secondly, a segment of the s. cerevisiae csd2 gene was used to screen an a. fumigatus lambda genomic dna library. the polymerase chain reaction (pcr)-based approach led to the identific ... | 1995 | 7854320 |
| localization of the gene for a novel human adenylyl cyclase (adcy7) to chromosome 16. | a novel form of human adenylyl cyclase (adcy7) has been discovered in the human erythroleukemia cell line (hel). this cell line has been widely used as a model for studies of the characteristics of human platelets. data from hel cells suggests that adcy7 may be the major ac form in human platelets. in the current study polymerase chain reaction (pcr) techniques coupled with use of human/rodent somatic hybrid panels and a yeast artificial chromosome (yac) library were used to determine the chromo ... | 1995 | 7860067 |
| a human protein selected for interference with ras function interacts directly with ras and competes with raf1. | the overexpression of some human proteins can cause interference with the ras signal transduction pathway in the yeast saccharomyces cerevisiae. the functional block is located at the level of the effector itself, since these proteins do not suppress activating mutations further downstream in the same pathway. we now demonstrate, with in vivo and in vitro experiments, that the protein encoded by one human cdna (clone 99) can interact directly with yeast ras2p and with human h-ras protein, and we ... | 1995 | 7862125 |
| the ligand-binding domains of the thyroid hormone/retinoid receptor gene subfamily function in vivo to mediate heterodimerization, gene silencing, and transactivation. | the ligand-binding domains (lbds) of the thyroid/retinoid receptor gene subfamily contain a series of heptad motifs important for dimeric interactions. this subfamily includes thyroid hormone receptors (t3rs), all-trans retinoic acid (ra) receptors (rars), 9-cis ra receptors (rars and retinoid x receptors [rxrs]), the 1,25-dihydroxyvitamin d3 receptor (vdr), and the receptors that modulate the peroxisomal beta-oxidation pathway (ppars). these receptors bind to their dna response elements in vitr ... | 1995 | 7862171 |
| amino acid sequence of rat kidney glutathione synthetase. | glutathione (gsh) synthetase [gamma-l-glutamyl-l-cysteine:glycine ligase (adp-forming), ec 6.3.2.3], an enzyme present in almost all cells, catalyzes the atp-dependent synthesis of gsh from gamma-l-glutamyl-l-cysteine and glycine. highly purified preparations of the enzyme have been obtained from rat kidney and several lower forms. the rat kidney enzyme (m(r), 118,000), which contains approximately 2% carbohydrate, is composed of two apparently identical subunits. the cdna encoding rat kidney gs ... | 1995 | 7862666 |
| mannosylphosphoryldolichol-mediated o-mannosylation of yeast glycoproteins: stereospecificity and recognition of the alpha-isoprene unit by a purified mannosyltransferase. | mannosylphosphoryldolichol (man-p-dol):protein o-mannosyltransferase (pmt1) was solubilized by extracting a crude microsomal fraction from saccharomyces cerevisiae with 1.2% chaps-0.5% desoxycholate and purified 120-fold by standard chromatographic procedures. these very stable, partially purified preparations of pmt1 catalyzed the transfer of mannosyl units from exogenous man-p-dol to serine/threonine residues in the synthetic peptide acceptor, tyr-asn-pro-thr-ser-val-nh2, forming o-mannosidic ... | 1995 | 7864633 |
| different subcellular localization of cytochrome b and the dormant nadph-oxidase in neutrophils and macrophages: effect on the production of reactive oxygen species during phagocytosis. | when neutrophils and macrophages phagocytose a prey, e.g., complement (c3b)-opsonized yeast particles, the oxygen radical generating nadph-oxidase is activated. in neutrophils, most of the production of oxygen metabolites occurred in an intracellular compartment, possibly in the phagolysosome. in contrast, no intracellular production could be detected in human macrophages. in these cells, the subcellular localization of the superoxide-generating nadph-oxidase and associated cytochrome b was asse ... | 1995 | 7867086 |
| degradation of humoral host defense by candida albicans proteinase. | the effect of an extracellular proteinase from the pathogenic yeast candida albicans on the bactericidal and opsonizing activities of human serum was studied. the ability of human polymorphonuclear leukocytes to kill staphylococcus aureus was greatly reduced when the bacteria were opsonized with human serum treated with the proteinase. the reduction in the opsonizing activity of human serum was attributed to degradation of the fc portion of immunoglobulin g by the action of c. albicans proteinas ... | 1995 | 7868271 |