Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| contrasting patterns of tolerance between chemical and biological insecticides in mosquitoes exposed to uv-a. | mosquitoes are vectors of major human diseases, such as malaria, dengue or yellow fever. because no efficient treatments or vaccines are available for most of these diseases, control measures rely mainly on reducing mosquito populations by the use of insecticides. numerous biotic and abiotic factors are known to modulate the efficacy of insecticides used in mosquito control. mosquito breeding sites vary from opened to high vegetation covered areas leading to a large ultraviolet gradient exposure ... | 2013 | 23911355 |
| efficacy of various larvicides against aedes aegypti immatures in the laboratory. | we conducted a laboratory study to evaluate the efficacy of control agents against small larvae, large larvae, and pupae of aedes aegypti to determine an appropriate larvicide regime to employ in emergency dengue control programs. the control agents included bacillus thuringiensis var. israelensis (bti), pyriproxyfen (an insect growth regulator), a larvicidal oil, aquatain amf (polydimethylsiloxane, a monomolecular film), and temephos at the recommend application dosages and rates. our results s ... | 2013 | 23883850 |
| increase in larval gut proteolytic activities and bti resistance in the dengue fever mosquito. | the bioinsecticide bacillus thuringiensis var. israelensis (bti) is increasingly used worldwide for mosquito control. although no established resistance to bti has been described in the field so far, a resistant aedes aegypti strain (litox strain) was selected in the laboratory using field-collected leaf litter containing bti toxins. this selected strain exhibits a moderate resistance level to bti, but a high resistance level to individual cry toxins. as bti contains four different toxins, gener ... | 2013 | 23192850 |
| effect of wolbachia on insecticide susceptibility in lines of aedes aegypti. | two stable infections of wolbachia pipientis, wmelpop and wmel, now established in aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. any effects of wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. bioassays of ae. aegypti were conducted to test for differences in response to insecticides between wolbachia infected (wmelpop, wmel) and uninfected lines. insecticides screened were bifenthrin, the pyret ... | 2013 | 23149015 |
| isolation of midgut escape mutants of two american genotype dengue 2 viruses from aedes aegypti. | several studies have shown that american genotype dengue 2 viruses (denv2) have reduced viral fitness in the mosquito vector, aedes aegypti, compared to other denv2 genotypes. diminished replication efficiency or inability to efficiently traverse membrane barriers encompassing organs such as the midgut or salivary glands are considered major factors negatively impacting viral fitness in the mosquito. | 2013 | 23937713 |
| wolbachia-associated bacterial protection in the mosquito aedes aegypti. | wolbachia infections confer protection for their insect hosts against a range of pathogens including bacteria, viruses, nematodes and the malaria parasite. a single mechanism that might explain this broad-based pathogen protection is immune priming, in which the presence of the symbiont upregulates the basal immune response, preparing the insect to defend against subsequent pathogen infection. a study that compared natural wolbachia infections in drosophila melanogaster with the mosquito vector ... | 2013 | 23951381 |
| analysis of lipids by gas-liquid chromatography and complementary methods in four strains of aedes aegypti mosquitoes. | fatty acid composition of total lipids, neutral lipids and phospholipids of strains of aedes aegypti were determined. the fatty acid composition of the strains differed quantitatively with regard to the relative percentage of commonly occurring fatty acids. gas-liquid chromatography of fatty acid methyl esters showed 18:1 (oleic or elaidic) to be the predominant fatty acid. the fatty acid was identified as oleic by argentation thin-layer chromatography. a modified colorimetric method was used to ... | 2013 | 4017543 |
| fever versus fever: the role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus. | two different species of flaviviruses, dengue virus (denv) and yellow fever virus (yfv), that originated in sylvatic cycles maintained in non-human primates and forest-dwelling mosquitoes have emerged repeatedly into sustained human-to-human transmission by aedes aegypti mosquitoes. sylvatic cycles of both viruses remain active, and where the two viruses overlap in west africa they utilize similar suites of monkeys and aedes mosquitoes. these extensive similarities render the differences in the ... | 2013 | 23523817 |
| analysis of early dengue virus infection in mice as modulated by aedes aegypti probing. | dengue virus (denv), the etiologic agent of dengue fever, is transmitted during probing of human skin by infected-mosquito bite. the expectorated viral inoculum also contains an assortment of mosquito salivary proteins that have been shown to modulate host hemostasis and innate immune responses. to examine the potential role of mosquito probing in denv establishment within the vertebrate host, we inoculated mice intradermally with denv serotype 2 strain 1232 at sites where aedes aegypti had or h ... | 2013 | 24198426 |
| eliciting renal failure in mosquitoes with a small-molecule inhibitor of inward-rectifying potassium channels. | mosquito-borne diseases such as malaria and dengue fever take a large toll on global health. the primary chemical agents used for controlling mosquitoes are insecticides that target the nervous system. however, the emergence of resistance in mosquito populations is reducing the efficacy of available insecticides. the development of new insecticides is therefore urgent. here we show that vu573, a small-molecule inhibitor of mammalian inward-rectifying potassium (kir) channels, inhibits a kir chan ... | 2013 | 23734226 |
| fluctuations at a low mean temperature accelerate dengue virus transmission by aedes aegypti. | environmental factors such as temperature can alter mosquito vector competence for arboviruses. results from recent studies indicate that daily fluctuations around an intermediate mean temperature (26°c) reduce vector competence of aedes aeygpti for dengue viruses (denv). theoretical predictions suggest that the mean temperature in combination with the magnitude of the diurnal temperature range (dtr) mediate the direction of these effects. | 2013 | 23638208 |
| reduction of aedes aegypti vector competence for dengue virus under large temperature fluctuations. | diurnal temperature fluctuations can fundamentally alter mosquito biology and mosquito-virus interactions in ways that impact pathogen transmission. we investigated the effect of two daily fluctuating temperature profiles on aedes aegypti vector competence for dengue virus (denv) serotype-1. a large diurnal temperature range of 18.6°c around a 26°c mean, corresponding with the low denv transmission season in northwestern thailand, reduced midgut infection rates and tended to extend the virus ext ... | 2013 | 23438766 |
| wolbachia versus dengue: evolutionary forecasts. | a novel form of biological control is being applied to the dengue virus. the agent is the maternally transmitted bacterium wolbachia, naturally absent from the main dengue vector, the mosquito aedes aegypti. three wolbachia-based control strategies have been proposed. one is suppression of mosquito populations by large-scale releases of males incompatible with native females; this intervention requires ongoing releases. the other interventions transform wild mosquito populations with wolbachia t ... | 2013 | 24481199 |
| promising aedes aegypti repellent chemotypes identified through integrated qsar, virtual screening, synthesis, and bioassay. | molecular field topology analysis, scaffold hopping, and molecular docking were used as complementary computational tools for the design of repellents for aedes aegypti, the insect vector for yellow fever, chikungunya, and dengue fever. a large number of analogues were evaluated by virtual screening with glide molecular docking software. this produced several dozen hits that were either synthesized or procured from commercial sources. analysis of these compounds by a repellent bioassay resulted ... | 2013 | 24039693 |
| the developmental transcriptome of the mosquito aedes aegypti, an invasive species and major arbovirus vector. | mosquitoes are vectors of a number of important human and animal diseases. the development of novel vector control strategies requires a thorough understanding of mosquito biology. to facilitate this, we used rna-seq to identify novel genes and provide the first high-resolution view of the transcriptome throughout development and in response to blood feeding in a mosquito vector of human disease, aedes aegypti, the primary vector for dengue and yellow fever. we characterized mrna expression at 3 ... | 2013 | 23833213 |
| mosquito protein kinase g phosphorylates flavivirus ns5 and alters flight behavior in aedes aegypti and anopheles gambiae. | many arboviral proteins are phosphorylated in infected mammalian cells, but it is unknown if the same phosphorylation events occur when insects are similarly infected. one of the mammalian kinases responsible for phosphorylation, protein kinase g (pkg), has been implicated in the behavior of multiple nonvector insects, but is unstudied in mosquitoes. pkg from aedes aegypti was cloned, and phosphorylation of specific viral sites was monitored by mass spectrometry from biochemical and cell culture ... | 2013 | 23930976 |
| stage-specific transcription during development of aedes aegypti. | aedes aegypti is the most important global vector of dengue virus infection in humans. availability of the draft genome sequence of this mosquito provides unique opportunities to study different aspects of its biology, including identification of genes and pathways relevant to the developmental processes associated with transition across individual life stages. however, detailed knowledge of gene expression patterns pertaining to developmental stages of a. aegypti is largely lacking. | 2013 | 23875547 |
| the toll and imd pathways are not required for wolbachia-mediated dengue virus interference. | wolbachia blocks dengue virus replication in drosophila melanogaster as well as in aedes aegypti. using the drosophila model and mutations in the toll and imd pathways, we showed that neither pathway is required for expression of the dengue virus-blocking phenotype in the drosophila host. this provides additional evidence that the mechanistic basis of wolbachia-mediated dengue virus blocking in insects is more complex than simple priming of the host insect innate immune system. | 2013 | 23986574 |
| the distribution of synonymous codon choice in the translation initiation region of dengue virus. | dengue is the most common arthropod-borne viral (arboviral) illness in humans. the genetic features concerning the codon usage of dengue virus (denv) were analyzed by the relative synonymous codon usage, the effective number of codons and the codon adaptation index. the evolutionary distance between denv and the natural hosts (homo sapiens, pan troglodytes, aedes albopictus and aedes aegypti) was estimated by a novel formula. finally, the synonymous codon usage preference for the translation ini ... | 2013 | 24204777 |
| towards a casa segura: a consumer product study of the effect of insecticide-treated curtains on aedes aegypti and dengue virus infections in the home. | the home, or domicile, is the principal environment for transmission of dengue virus (denv) between humans and mosquito vectors. community-wide distribution of insecticide-treated curtains (itcs), mimicking vector control program-driven interventions, has shown promise to reduce denv infections. we conducted a casa segura consumer product intervention study in mérida, mexico to determine the potential to reduce intradomicillary denv transmission through itc use in individual homes. dengue virus ... | 2013 | 23732254 |
| regulation of aedes aegypti population dynamics in field systems: quantifying direct and delayed density dependence. | transgenic strains of aedes aegypti have been engineered to help control transmission of dengue virus. although resources have been invested in developing the strains, we lack data on the ecology of mosquitoes that could impact the success of this approach. although studies of intra-specific competition have been conducted using ae. aegypti larvae, none of these studies examine mixed age cohorts at densities that occur in the field, with natural nutrient levels. experiments were conducted in mex ... | 2013 | 23669230 |
| gravitraps for management of dengue clusters in singapore. | although singapore has an intensive dengue control program, dengue remains endemic with regular outbreaks. we report development and use of a novel adult oviposition trap, the gravitrap, in managing dengue cluster areas. the gravitrap is a simple, hay infusion-filled cylindrical trap with a sticky inner surface to serve as an oviposition site for gravid female aedes mosquitoes. wire gauze fitted above the water level minimizes the risk of it being an unwanted breeding habitat. the gravitrap was ... | 2013 | 23478581 |
| vectorial capacity of aedes aegypti for dengue virus type 2 is reduced with co-infection of metarhizium anisopliae. | aedes aegypti, is the major dengue vector and a worldwide public health threat combated basically by chemical insecticides. in this study, the vectorial competence of ae. aegypti co-infected with a mildly virulent metarhizium anisopliae and fed with blood infected with the denv-2 virus, was examined. | 2013 | 23505581 |
| modeling dengue vector dynamics under imperfect detection: three years of site-occupancy by aedes aegypti and aedes albopictus in urban amazonia. | aedes aegypti and ae. albopictus are the vectors of dengue, the most important arboviral disease of humans. to date, aedes ecology studies have assumed that the vectors are truly absent from sites where they are not detected; since no perfect detection method exists, this assumption is questionable. imperfect detection may bias estimates of key vector surveillance/control parameters, including site-occupancy (infestation) rates and control intervention effects. we used a modeling approach that e ... | 2013 | 23472194 |
| contact irritant responses of aedes aegypti using sublethal concentration and focal application of pyrethroid chemicals. | previous studies have demonstrated contact irritant and spatial repellent behaviors in aedes aegypti following exposure to sublethal concentrations of chemicals. these sublethal actions are currently being evaluated in the development of a push-pull strategy for ae. aegypti control. this study reports on mosquito escape responses after exposure to candidate chemicals for a contact irritant focused push-pull strategy using varying concentrations and focal application. | 2013 | 23469302 |
| detection of denv-4 genotype i from mosquitoes collected in the city of manaus, brazil. | dengue epidemics have been reported in brazil since 1981. in manaus, a large city in the amazon region, dengue is endemic with all four-virus serotypes (denv-1, -2, -3, and -4) simultaneously causing human disease. in 2008, during a surveillance of dengue virus in mosquitoes in the district of tancredo neves in manaus, 260 mosquitoes of aedes genus were captured, identified and grouped into pools of 10 mosquitoes. | 2013 | 23421733 |
| spatial patterns of high aedes aegypti oviposition activity in northwestern argentina. | in argentina, dengue has affected mainly the northern provinces, including salta. the objective of this study was to analyze the spatial patterns of high aedes aegypti oviposition activity in san ramón de la nueva orán, northwestern argentina. the location of clusters as hot spot areas should help control programs to identify priority areas and allocate their resources more effectively. | 2013 | 23349813 |
| glytube: a conical tube and parafilm m-based method as a simplified device to artificially blood-feed the dengue vector mosquito, aedes aegypti. | aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. these feeders are also the best method for experimental oral infection of ae. aegypti with dengue viruses. however, most of them are expensive or laborious to construct. based on principle of rutledge-type feeder, a conventional conical tube, glycerol and parafilm-m were used to develop a simpl ... | 2013 | 23342010 |
| field trial on a novel control method for the dengue vector, aedes aegypti by the systematic use of olyset® net and pyriproxyfen in southern vietnam. | jars, tanks, and drums provide favorable rearing/breeding sites for aedes aegypti in vietnam. however, the use of insecticides to control mosquitoes at such breeding sites has not been approved in vietnam since they are also often sources of drinking water, making larval vector control difficult. mosquito nets pre-treated with long-lasting insecticide treated nets (llitns) form an effective measure for malaria control. we examined changes in the abundance of immature aedes aegypti to evaluate th ... | 2013 | 23312018 |
| the influence of dengue virus serotype-2 infection on aedes aegypti (diptera: culicidae) motivation and avidity to blood feed. | dengue virus (denv) is transmitted by aedes aegypti, a species that lives in close association with human dwellings. the behavior of denv-infected mosquitoes needs further investigation, especially regarding the potential influence of denv on mosquito biting motivation and avidity. | 2013 | 23755202 |
| differential expression of apoptosis related genes in selected strains of aedes aegypti with different susceptibilities to dengue virus. | aedes aegypti is the principal vector of dengue viruses worldwide. we identified field collected insects with differential susceptibility to dengue-2 virus (denv-2) and used isofemale selection to establish susceptible and refractory strains based on midgut infection barriers. previous experiments had identified higher expression of apoptosis-related genes in the refractory strain. to identify potential molecular mechanisms associated with denv susceptibility, we evaluated the differential expre ... | 2013 | 23593426 |
| age-dependent effects of oral infection with dengue virus on aedes aegypti (diptera: culicidae) feeding behavior, survival, oviposition success and fecundity. | aedes aegypti is the main vector of dengue, a disease that is increasing its geographical range as well as incidence rates. despite its public health importance, the effect of dengue virus (denv) on some mosquito traits remains unknown. here, we investigated the impact of denv-2 infection on the feeding behavior, survival, oviposition success and fecundity of ae. aegypti females. | 2013 | 23555838 |
| mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. | dengue virus (denv), a flavivirus of global importance, is transmitted to humans by mosquitoes. in this study, we developed in vitro and in vivo models of saliva-mediated enhancement of denv infectivity. serine protease activity in aedes aegypti saliva augmented virus infectivity in vitro by proteolyzing extracellular matrix proteins, thereby increasing viral attachment to heparan sulfate proteoglycans and inducing cell migration. a serine protease inhibitor reduced saliva-mediated enhancement o ... | 2013 | 24131723 |
| genetic variation in the 3' untranslated region of dengue virus serotype 3 strains isolated from mosquitoes and humans in brazil. | dengue, a mosquito-borne viral infection caused by one of the four dengue virus (denv) serotypes (denv-1 to 4), replicate alternately on the mosquito vector and human host and are responsible for infections throughout tropical and subtropical regions of the world. in brazil, the disease has become a major public health problem and the introduction of denv-3 in 2000 in rio de janeiro (rj) was associated with severe dengue epidemics. the potential emergence of strains associated with severe diseas ... | 2013 | 23282086 |
| identification of new protein interactions between dengue fever virus and its hosts, human and mosquito. | the four divergent serotypes of dengue virus are the causative agents of dengue fever, dengue hemorrhagic fever and dengue shock syndrome. about two-fifths of the world's population live in areas where dengue is prevalent, and thousands of deaths are caused by the viruses every year. dengue virus is transmitted from one person to another primarily by the yellow fever mosquito, aedes aegypti. recent studies have begun to define how the dengue viral proteins interact with host proteins to mediate ... | 2013 | 23326450 |
| first isolation of dengue virus from the 2010 epidemic in nepal. | dengue is an emerging disease in nepal and was first observed as an outbreak in nine lowland districts in 2006. in 2010, however, a large epidemic of dengue occurred with 4,529 suspected and 917 serologically-confirmed cases and five deaths reported in government hospitals in nepal. the collection of demographic information was performed along with an entomological survey and clinical evaluation of the patients. a total of 280 serum samples were collected from suspected dengue patients. these sa ... | 2013 | 24155651 |
| development of guidelines for the surveillance of invasive mosquitoes in europe. | the recent notifications of autochthonous cases of dengue and chikungunya in europe prove that the region is vulnerable to these diseases in areas where known mosquito vectors (aedes albopictus and aedes aegypti) are present. strengthening surveillance of these species as well as other invasive container-breeding aedine mosquito species such as aedes atropalpus, aedes japonicus, aedes koreicus and aedes triseriatus is therefore required. in order to support and harmonize surveillance activities ... | 2013 | 23866915 |
| genetic and phenotypic characterization of manufacturing seeds for a tetravalent dengue vaccine (denvax). | we have developed a manufacturing strategy that can improve the safety and genetic stability of recombinant live-attenuated chimeric dengue vaccine (denvax) viruses. these viruses, containing the pre-membrane (prm) and envelope (e) genes of dengue serotypes 1-4 in the replicative background of the attenuated dengue-2 pdk-53 vaccine virus candidate, were manufactured under cgmp. | 2013 | 23738026 |
| host and viral features of human dengue cases shape the population of infected and infectious aedes aegypti mosquitoes. | dengue is the most prevalent arboviral disease of humans. the host and virus variables associated with dengue virus (denv) transmission from symptomatic dengue cases (n = 208) to aedes aegypti mosquitoes during 407 independent exposure events was defined. the 50% mosquito infectious dose for each of denv-1-4 ranged from 6.29 to 7.52 log10 rna copies/ml of plasma. increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of denv trans ... | 2013 | 23674683 |
| extensive circadian and light regulation of the transcriptome in the malaria mosquito anopheles gambiae. | mosquitoes exhibit 24 hr rhythms in flight activity, feeding, reproduction and development. to better understand the molecular basis for these rhythms in the nocturnal malaria vector anopheles gambiae, we have utilized microarray analysis on time-of-day specific collections of mosquitoes over 48 hr to explore the coregulation of gene expression rhythms by the circadian clock and light, and compare these with the 24 hr rhythmic gene expression in the diurnal aedes aegypti dengue vector mosquito. | 2013 | 23552056 |
| an explosive epidemic of denv-3 in cairns, australia. | from november 2008-may 2009 cairns queensland australia was struck by an explosive epidemic of denv-3 that exceeded the capacity of highly skilled dengue control team to control it. we describe the environmental, virological and entomological factors associated with this outbreak to better understand the circumstances leading to its occurrence. patient interviews, serological results and viral sequencing strongly suggest that the imported index case was infected in kalimantan, indonesia. a delay ... | 2013 | 23874522 |
| transcriptomic profiling of diverse aedes aegypti strains reveals increased basal-level immune activation in dengue virus-refractory populations and identifies novel virus-vector molecular interactions. | genetic variation among aedes aegypti populations can greatly influence their vector competence for human pathogens such as the dengue virus (denv). while intra-species transcriptome differences remain relatively unstudied when compared to coding sequence polymorphisms, they also affect numerous aspects of mosquito biology. comparative molecular profiling of mosquito strain transcriptomes can therefore provide valuable insight into the regulation of vector competence. we established a panel of a ... | 2013 | 23861987 |
| wolbachia uses a host microrna to regulate transcripts of a methyltransferase, contributing to dengue virus inhibition in aedes aegypti. | the endosymbiont wolbachia is common among insects and known for the reproductive manipulations it exerts on hosts as well as inhibition of virus replication in their hosts. recently, we showed that wolbachia uses host micrornas to manipulate host gene expression for its efficient maintenance in the dengue mosquito vector, aedes aegypti. cytosine methylation is mediated by a group of proteins called dna (cytosine-5) methyltransferases, which are structurally and functionally conserved from proka ... | 2013 | 23733960 |
| is there a risk of yellow fever virus transmission in south asian countries with hyperendemic dengue? | the fact that yellow fever (yf) has never occurred in asia remains an "unsolved mystery" in global health. most countries in asia with high aedes aegypti mosquito density are considered "receptive" for yf transmission. recently, health officials in sri lanka issued a public health alert on the potential spread of yf from a migrant group from west africa. we performed an extensive review of literature pertaining to the risk of yf in sri lanka/south asian region to understand the probability of ac ... | 2013 | 24367789 |
| mosquito vector diversity across habitats in central thailand endemic for dengue and other arthropod-borne diseases. | recent years have seen the greatest ecological disturbances of our times, with global human expansion, species and habitat loss, climate change, and the emergence of new and previously-known infectious diseases. biodiversity loss affects infectious disease risk by disrupting normal relationships between hosts and pathogens. mosquito-borne pathogens respond to changing dynamics on multiple transmission levels and appear to increase in disturbed systems, yet current knowledge of mosquito diversity ... | 2013 | 24205420 |
| temephos resistance in aedes aegypti in colombia compromises dengue vector control. | control and prevention of dengue relies heavily on the application of insecticides to control dengue vector mosquitoes. in colombia, application of the larvicide temephos to the aquatic breeding sites of aedes aegypti is a key part of the dengue control strategy. resistance to temephos was recently detected in the dengue-endemic city of cucuta, leading to questions about its efficacy as a control tool. here, we characterize the underlying mechanisms and estimate the operational impact of this re ... | 2013 | 24069492 |
| requirement for commissureless2 function during dipteran insect nerve cord development. | in drosophila melanogaster, commissureless (comm) function is required for proper nerve cord development. although comm orthologs have not been identified outside of drosophila species, some insects possess orthologs of drosophila comm2, which may also regulate embryonic nerve cord development. here, this hypothesis is explored through characterization of comm2 genes in two disease vector mosquitoes. | 2013 | 24026811 |
| mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, streptomyces sp. m25 against anopheles subpictus, culex quinquefasciatus and aedes aegypti. | the present work reports the larvicidal potential of microbially synthesised silver nanoparticles (agnps) by using an actinobacterium, streptomyces sp. m25 isolated from western ghats, tamil nadu, india. the biomass of streptomyces sp. was exposed to 1 mm silver nitrate (agno3) solution. the synthesis of agnps was confirmed by visual inspection followed by instrumental analysis such as uv-vis spectroscopy, fourier transform infrared spectroscopy, x-ray diffraction (xrd), energy dispersive x-ray ... | 2013 | 26688633 |
| confusion, knock-down and kill of aedes aegypti using metofluthrin in domestic settings: a powerful tool to prevent dengue transmission? | dengue control methods are reliant upon control of the vector, primarily aedes aegypti. current adulticiding methods in north queensland include treating premises with residual synthetic pyrethroid insecticides (interior residual spraying; irs), a laborious, intrusive task. the vapor active synthetic pyrethroid metofluthrin might offer an efficient alternative as some studies indicate that it prevents biting and has strong knockdown effects. however, its expellant and/or irritant effects, longev ... | 2013 | 24025232 |
| functional and genetic characterization of neuropeptide y-like receptors in aedes aegypti. | female aedes aegypti mosquitoes are the principal vector for dengue fever, causing 50-100 million infections per year, transmitted between human and mosquito by blood feeding. ae. aegypti host-seeking behavior is known to be inhibited for three days following a blood meal by a hemolymph-borne humoral factor. head peptide-i is a candidate peptide mediating this suppression, but the mechanism by which this peptide alters mosquito behavior and the receptor through which it signals are unknown. | 2013 | 24130914 |
| evidence for gene duplication in the voltage-gated sodium channel gene of aedes aegypti. | mutations in the voltage-gated sodium channel gene (nav), known as kdr mutations, are associated with pyrethroid and ddt insecticide resistance in a number of species. in the mosquito dengue vector aedes aegypti, besides kdr, other polymorphisms allowed grouping aanav sequences as type 'a' or 'b'. here, we point a series of evidences that these polymorphisms are actually involved in a gene duplication event. | 2013 | 24481195 |
| testing fungus impregnated cloths for the control of adult aedes aegypti under natural conditions. | entomopathogenic fungi could be useful tools for reducing populations of the dengue mosquito aedes aegypti. here the efficiency of fungus (metarhizium anisopliae) impregnated cloths (with and without imidacloprid [imi]) was evaluated against adult a. aegypti in simulated human dwellings. behaviour of mosquitoes in the presence of black cloths was also investigated. | 2013 | 24010874 |
| effects of fluctuating daily temperatures at critical thermal extremes on aedes aegypti life-history traits. | the effect of temperature on insect biology is well understood under constant temperature conditions, but less so under more natural, fluctuating conditions. a fluctuating temperature profile around a mean of 26°c can alter aedes aegypti vector competence for dengue viruses as well as numerous life-history traits, however, the effect of fluctuations on mosquitoes at critical thermal limits is unknown. | 2013 | 23520534 |
| atypical manifestations of dengue fever (df) - where do we stand today? | dengue fever (df) is transmitted by aedes aegypti mosquitoes. with growing population, rapid urbanization and lack of appropriate sanitary measures, proliferation of mosquitoes and subsequent dengue infections have increased rampantly with an estimated 30-fold increase in incidence over last five decades. with rising disease burden, atypical manifestations have increased as well, which are missed most often due to lack of awareness. our aim was to look for the atypical manifestations of dengue f ... | 2013 | 24596727 |
| innate immunity to dengue virus infection and subversion of antiviral responses. | dengue is a major public health issue in tropical and subtropical regions worldwide. the four serotypes of dengue virus (denv1-denv4) are spread primarily by aedes aegypti and aedes albopictus mosquitoes, whose geographic range continues to expand. humans are the only host for epidemic strains of denv, and the virus has developed sophisticated mechanisms to evade human innate immune responses. the host cell's first line of defense begins with an intracellular signaling cascade resulting in produ ... | 2013 | 24316047 |
| distribution of mosquito larvae on kosrae island, kosrae state, the federated states of micronesia. | surveys of mosquito larvae were carried out in six areas of kosrae island, kosrae state, the federated states of micronesia in december 2009 and june 2012. a total of 962 larvae of six species were collected from 106 natural and artificial habitats. they were identified as aedes aegypti, ae. albopictus, ae. marshallensis, culex quinquefasciatus, cx. annulirostris, and cx. kusaiensis. this is the first report from kosrae island for three of these species-ae. marshallensis, cx. quinquefasciatus, a ... | 2013 | 24478593 |
| dengue in the united states of america: a worsening scenario? | dengue is a febrile illness caused by any of the four dengue virus types (denv-1 to -4, genus flavivirus, family flaviviridae) mainly transmitted by the mosquito aedes aegypti. denv can be transmitted by blood transfusion. dengue has been historically present in the continental united states (us), in the state of hawaii, and in the us insular territories in the caribbean and the pacific. during the second half of the 20th century, most of the cases reported in the us were imported cases brought ... | 2013 | 23865061 |
| proteomic biomarkers for ageing the mosquito aedes aegypti to determine risk of pathogen transmission. | biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. using the 2-d difference gel electrophoresis (2-d dige) procedure, we investigated the abundance of up to 898 proteins from the yellow fever and dengue virus vector, aedes aegypti, during ageing. by applying a mixed-effects model of protein expression, we identified five common patterns of abundance change du ... | 2013 | 23536806 |
| detection of arboviruses and other micro-organisms in experimentally infected mosquitoes using massively parallel sequencing. | human disease incidence attributed to arbovirus infection is increasing throughout the world, with effective control interventions limited by issues of sustainability, insecticide resistance and the lack of effective vaccines. several promising control strategies are currently under development, such as the release of mosquitoes trans-infected with virus-blocking wolbachia bacteria. implementation of any control program is dependent on effective virus surveillance and a thorough understanding of ... | 2013 | 23460918 |
| apropos "vaccination for safe travel to india". | 2013 | 24356443 | |
| a threshold analysis of dengue transmission in terms of weather variables and imported dengue cases in australia. | dengue virus (denv) transmission in australia is driven by weather factors and imported dengue fever (df) cases. however, uncertainty remains regarding the threshold effects of high-order interactions among weather factors and imported df cases and the impact of these factors on autochthonous df. a time-series regression tree model was used to assess the threshold effects of natural temporal variations of weekly weather factors and weekly imported df cases in relation to incidence of weekly auto ... | 2013 | 26038449 |
| modelling adult aedes aegypti and aedes albopictus survival at different temperatures in laboratory and field settings. | the survival of adult female aedes mosquitoes is a critical component of their ability to transmit pathogens such as dengue viruses. one of the principal determinants of aedes survival is temperature, which has been associated with seasonal changes in aedes populations and limits their geographical distribution. the effects of temperature and other sources of mortality have been studied in the field, often via mark-release-recapture experiments, and under controlled conditions in the laboratory. ... | 2013 | 24330720 |
| the relationship between economic status, knowledge on dengue, risk perceptions and practices. | the reemergence of dengue as an important public health problem reflects the difficulties in sustaining vertically organized, effective, control programs and the need for community-based strategies for aedes aegypti control that result in behavioral change. we aimed to disentangle the relationships between underlying determinants of dengue related practices. we conducted a cross-sectional study in 780 households in la lisa, havana, cuba. a questionnaire and an observation guide were administrate ... | 2013 | 24349145 |
| human impacts have shaped historical and recent evolution in aedes aegypti, the dengue and yellow fever mosquito. | although anthropogenic impacts are often considered harmful to species, human modifications to the landscape can actually create novel niches to which other species can adapt. these "domestication" processes are especially important in the context of arthropod disease vectors, where ecological overlap of vector and human populations may lead to epidemics. here, we present results of a global genetic study of one such species, the dengue and yellow fever mosquito, aedes aegypti, whose evolutionar ... | 2013 | 24111703 |
| proteomic identification of dengue virus binding proteins in aedes aegypti mosquitoes and aedes albopictus cells. | the main vector of dengue in america is the mosquito aedes aegypti, which is infected by dengue virus (denv) through receptors of midgut epithelial cells. the envelope protein (e) of dengue virus binds to receptors present on the host cells through its domain iii that has been primarily recognized to bind cell receptors. in order to identify potential receptors, proteins from mosquito midgut tissue and c6/36 cells were purified by affinity using columns with the recombinant e protein domain iii ... | 2013 | 24324976 |
| the susceptibility of aedes aegypti populations displaying temephos resistance to bacillus thuringiensis israelensis: a basis for management. | aedes aegypti is the vector of dengue virus, and its control is essential to prevent disease transmission. among the agents available to control this species, biolarvicides based on bacillus thuringiensis serovar israelensis (bti) are an effective alternative to replace the organophosphate temephos for controlling populations that display resistance to this insecticide. the major goal of this study was to determine the baseline susceptibility of brazilian ae. aegypti populations to bti, taking i ... | 2013 | 24499507 |
| aedes mosquito species in western saudi arabia. | the aedes meigen (diptera: culicidae) mosquito species populations in the western region of saudi arabia, especially in and around jeddah, are increasing, therefore increasing susceptibility of humans to the dengue virus. an extensive survey was carried out for one year, and four species were identified with the help of different pictorial keys available. the identification was based on morphological characteristics of adult female aedes mosquitoes. | 2014 | 25373216 |
| field evaluation of natural human odours and the biogent-synthetic lure in trapping aedes aegypti, vector of dengue and chikungunya viruses in kenya. | methods currently used in sampling adult aedes aegypti, the main vector of dengue and chikungunya viruses are limited for effective surveillance of the vector and accurate determination of the extent of virus transmission during outbreaks and inter - epidemic periods. here, we document the use of natural human skin odours in baited traps to improve sampling of adult ae. aegypti in two different endemic areas of chikungunya and dengue in kenya - kilifi and busia counties. the chemistry of the vol ... | 2014 | 25246030 |
| chikungunya in the caribbean: an epidemic in the making. | chikungunya is a mosquito-borne virus that has shown increased prevalence in the caribbean since october 2013. there have been several outbreaks throughout asian and african countries over the past few decades with global travel and tourism having a major impact on the further spread of this disease. improved policies and practices for preventative measures and epidemiological surveillance must be implemented to prevent the continued transmission of chikungunya. | 2014 | 25245516 |
| high level of vector competence of aedes aegypti and aedes albopictus from ten american countries as a crucial factor in the spread of chikungunya virus. | chikungunya virus (chikv) causes a major public health problem. in 2004, chikv began an unprecedented global expansion and has been responsible for epidemics in africa, asia, islands in the indian ocean region, and surprisingly, in temperate regions, such as europe. intriguingly, no local transmission of chikungunya virus (chikv) had been reported in the americas until recently, despite the presence of vectors and annually reported imported cases. here, we assessed the vector competence of 35 am ... | 2014 | 24672026 |
| fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse aedes aegypti strain. | in 2006, we reported a mariner (mos1)-transformed aedes aegypti line, carb77, which was highly resistant to dengue-2 virus (denv2). carb77 mosquitoes expressed a denv2-specific inverted-repeat (ir) rna in midgut epithelial cells after ingesting an infectious bloodmeal. the ir-rna formed double-stranded denv2-derived rna, initiating an intracellular antiviral rna interference (rnai) response. however, carb77 mosquitoes stopped expressing the ir-rna after 17 generations in culture and lost their d ... | 2014 | 24810399 |
| genetic shifting: a novel approach for controlling vector-borne diseases. | rendering populations of vectors of diseases incapable of transmitting pathogens through genetic methods has long been a goal of vector geneticists. we outline a method to achieve this goal that does not involve the introduction of any new genetic variants to the target population. rather we propose that shifting the frequencies of naturally occurring alleles that confer refractoriness to transmission can reduce transmission below a sustainable level. the program employs methods successfully use ... | 2014 | 24794113 |
| dendritic cells in dengue virus infection: targets of virus replication and mediators of immunity. | dendritic cells (dcs) are sentinels of the immune system and detect pathogens at sites of entry, such as the skin. in addition to the ability of dcs to control infections directly via their innate immune functions, dcs help to prime adaptive b- and t-cell responses by processing and presenting antigen in lymphoid tissues. infected aedes aegypti or aedes albopictus mosquitoes transmit the four dengue virus (denv) serotypes to humans while probing for small blood vessels in the skin. denv causes t ... | 2014 | 25566258 |
| socially structured human movement shapes dengue transmission despite the diffusive effect of mosquito dispersal. | for sexually and directly transmitted infectious diseases, social connections influence transmission because they determine contact between individuals. for pathogens that are indirectly transmitted by arthropod vectors, the movement of the vectors is thought to diminish the role of social connections. results from a recent study of mosquito-borne dengue virus (denv), however, indicate that human movement alone can explain significant spatial variation in urban transmission rates. because moveme ... | 2014 | 24593919 |
| ecological, biological and social dimensions of dengue vector breeding in five urban settings of latin america: a multi-country study. | dengue is an increasingly important public health problem in most latin american countries and more cost-effective ways of reducing dengue vector densities to prevent transmission are in demand by vector control programs. this multi-centre study attempted to identify key factors associated with vector breeding and development as a basis for improving targeted intervention strategies. | 2014 | 24447796 |
| burchellin: study of bioactivity against aedes aegypti. | the dengue mosquito aedes aegypti linnaeus, 1762 is a widespread insect pest of serious medical importance. since no effective vaccine is available for treating dengue, the eradication or control of the main mosquito vector is regarded as essential. since conventional insecticides have limited success, plants may be an alternative source of larvicidal agents, since they contain a rich source of bioactive chemicals. the aim of this study was to evaluate the larvicidal activity of the neolignan bu ... | 2014 | 24713267 |
| in silico models for predicting vector control chemicals targeting aedes aegypti. | human arboviral diseases have emerged or re-emerged in numerous countries worldwide due to a number of factors including the lack of progress in vaccine development, lack of drugs, insecticide resistance in mosquitoes, climate changes, societal behaviours, and economical constraints. thus, aedes aegypti is the main vector of the yellow fever and dengue fever flaviviruses and is also responsible for several recent outbreaks of the chikungunya alphavirus. as for the other mosquito species, the a. ... | 2014 | 25275884 |
| larvicidal, repellent, and irritant potential of the seed-derived essential oil of apium graveolens against dengue vector, aedes aegypti l. (diptera: culicidae). | aedes aegypti l. is one of the primary disease vectors spreading various dreadful diseases throughout the world, specifically over tropics and subtropics. keeping in view the adverse effects of chemical insecticides-based intervention measures, the eco-friendly and bio-degradable essential oil extracted from the seeds of celery, apium graveolens were investigated for its efficacy against ae. aegypti. larvicidal bioassay carried out with the seed oil against early fourth instars of ae. aegypti ca ... | 2014 | 25279371 |
| mating competitiveness and life-table comparisons between transgenic and indian wild-type aedes aegypti l. | ox513a is a genetically engineered strain of aedes aegypti carrying a repressible, dominantly inherited transgene that confers lethality in immature heterozygous progeny. released male ox513a adults have proven to be effective for the localised suppression of wild ae. aegypti, highlighting its potential in vector control. mating and life-table assessments were used to compare ox513a with reared ae. aegypti strains collected from new delhi and aurangabad regions in india. | 2014 | 25078081 |
| collagen-binding protein, aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, aedes aegypti. | mosquito salivary glands have important roles in blood feeding and pathogen transmission. however, the biological relevance of many salivary components has yet to be determined. aegyptin, a secreted salivary protein from aedes aegypti, binds collagen and inhibits platelet aggregation and adhesion. we used a transgenic approach to study the relevance of aegyptin in mosquito blood feeding. aedes aegypti manipulated genetically to express gene-specific inverted-repeat rna sequences exhibited signif ... | 2014 | 24778255 |
| criteria for identifying and evaluating candidate sites for open-field trials of genetically engineered mosquitoes. | recent laboratory successes in the development of genetically engineered mosquitoes for controlling pathogen transmission have fostered the need for standardized procedures for advancing the technical achievements to practical tools. it is incumbent in many cases for the same scientists doing the in-laboratory discovery research to also take on the initial challenges of developing the pathway that will move the technologies to the field. one of these challenges is having a set of criteria for se ... | 2014 | 24689963 |
| human to mosquito transmission of dengue viruses. | the successful transmission of dengue virus from a human host to a mosquito vector requires a complex set of factors to align. it is becoming increasingly important to improve our understanding of the parameters that shape the human to mosquito component of the transmission cycle so that vaccines and therapeutic antivirals can be fully evaluated and epidemiological models refined. here we describe these factors, and discuss the biological and environmental impacts and demographic changes that ar ... | 2014 | 24987394 |
| limited dengue virus replication in field-collected aedes aegypti mosquitoes infected with wolbachia. | dengue is one of the most widespread mosquito-borne diseases in the world. the causative agent, dengue virus (denv), is primarily transmitted by the mosquito aedes aegypti, a species that has proved difficult to control using conventional methods. the discovery that a. aegypti transinfected with the wmel strain of wolbachia showed limited denv replication led to trial field releases of these mosquitoes in cairns, australia as a biocontrol strategy for the virus. | 2014 | 24587459 |
| genome-wide snps lead to strong signals of geographic structure and relatedness patterns in the major arbovirus vector, aedes aegypti. | genetic markers are widely used to understand the biology and population dynamics of disease vectors, but often markers are limited in the resolution they provide. in particular, the delineation of population structure, fine scale movement and patterns of relatedness are often obscured unless numerous markers are available. to address this issue in the major arbovirus vector, the yellow fever mosquito (aedes aegypti), we used double digest restriction-site associated dna (ddrad) sequencing for t ... | 2014 | 24726019 |
| undesirable consequences of insecticide resistance following aedes aegypti control activities due to a dengue outbreak. | during a dengue outbreak with co-circulation of denv-1 and -2 in the city of boa vista, one patient was diagnosed with denv-4, a serotype supposed absent from brazil for almost 30 years. the re-emergence of denv-4 triggered the intensification of mechanical and chemical aedes aegypti control activities in order to reduce vector density and avoid denv-4 dissemination throughout the country. | 2014 | 24676277 |
| differential susceptibility of two field aedes aegypti populations to a low infectious dose of dengue virus. | the infectious dose required to infect mosquito vectors when they take a blood meal from a viremic person is a critical parameter underlying the probability of dengue virus (denv) transmission. because experimental vector competence studies typically examine the proportion of mosquitoes that become infected at intermediate or high denv infectious doses in the blood meal, the minimum blood meal titer required to infect mosquitoes is poorly documented. understanding the factors influencing the low ... | 2014 | 24664142 |
| identification of carboxylesterase genes implicated in temephos resistance in the dengue vector aedes aegypti. | thailand is currently experiencing one of its worst dengue outbreaks in decades. as in most countries where this disease is endemic, dengue control in thailand is largely reliant on the use of insecticides targeting both immature and adult stages of the aedes mosquito, with the organophosphate insecticide, temephos, being the insecticide of choice for attacking the mosquito larvae. resistance to temephos was first detected in aedes aegypti larvae in thailand approximately 25 years ago but the me ... | 2014 | 24651719 |
| stability of the wmel wolbachia infection following invasion into aedes aegypti populations. | the wmel infection of drosophila melanogaster was successfully transferred into aedes aegypti mosquitoes where it has the potential to suppress dengue and other arboviruses. the infection was subsequently spread into two natural populations at yorkeys knob and gordonvale near cairns, queensland in 2011. here we report on the stability of the infection following introduction and we characterize factors influencing the ongoing dynamics of the infection in these two populations. while the wolbachia ... | 2014 | 25211492 |
| lineage ii of southeast asian/american denv-2 is associated with a severe dengue outbreak in the peruvian amazon. | during 2010 and 2011, the loreto region of peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. this outbreak coincided with the reappearance of dengue virus-2 (denv-2) in loreto after almost 8 years. whole-genome sequence indicated that denv-2 from the outbreak belonged to lineage ii of the southeast asian/american genotype and was most closely related to viruses circulating in brazil during 2007 and 2008, whereas denv-2 previously circulating in loreto gro ... | 2014 | 25002298 |
| comparative analysis of response to selection with three insecticides in the dengue mosquito aedes aegypti using mrna sequencing. | mosquito control programmes using chemical insecticides are increasingly threatened by the development of resistance. such resistance can be the consequence of changes in proteins targeted by insecticides (target site mediated resistance), increased insecticide biodegradation (metabolic resistance), altered transport, sequestration or other mechanisms. as opposed to target site resistance, other mechanisms are far from being fully understood. indeed, insecticide selection often affects a large n ... | 2014 | 24593293 |
| insecticidal activity of leptodactylus knudseni and phyllomedusa vaillantii crude skin secretions against the mosquitoes anopheles darlingi and aedes aegypti. | mosquitoes are important vectors of several diseases, including malaria and dengue, and control measures are mostly performed using chemical insecticides. unfortunately, mosquito resistance to commonly applied insecticides is widespread. therefore, a prospection for new molecules with insecticidal activity based on amazon biodiversity using the anurans leptodactylus knudseni and phyllomedusa vaillantii was performed against the mosquito species anopheles darlingi and aedes aegypti. | 2014 | 25165469 |
| preliminary evaluation on the efficiency of the kit platelia dengue ns1 ag-elisa to detect dengue virus in dried aedes aegypti: a potential tool to improve dengue surveillance. | surveillance is a critical component of any dengue prevention and control programme. herein, we investigate the efficiency of the commercial kit platelia dengue ns1 ag-elisa to detect dengue virus (denv) antigens in aedes aegypti mosquitoes infected under laboratory conditions. | 2014 | 24690324 |
| effect of dengue-2 virus infection on protein expression in the salivary glands of aedes aegypti mosquitoes. | dengue virus (denv) is the most important mosquito-transmitted flavivirus that is transmitted throughout the tropical and subtropical regions of the world. the primary mosquito vector of denv in urban locations is aedes aegypti. key to understanding the transmission of denv is the relationship between pathogen and vector. accordingly, we report our preliminary characterization of the differentially expressed proteins from ae. aegypti mosquitoes after denv infection. we investigated the virus-vec ... | 2014 | 24445208 |
| vectorial capacity of aedes aegypti: effects of temperature and implications for global dengue epidemic potential. | dengue is a mosquito-borne viral disease that occurs mainly in the tropics and subtropics but has a high potential to spread to new areas. dengue infections are climate sensitive, so it is important to better understand how changing climate factors affect the potential for geographic spread and future dengue epidemics. vectorial capacity (vc) describes a vector's propensity to transmit dengue taking into account human, virus, and vector interactions. vc is highly temperature dependent, but most ... | 2014 | 24603439 |
| clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil. | dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously. | 2014 | 24978469 |
| increased replicative fitness of a dengue virus 2 clade in native mosquitoes: potential contribution to a clade replacement event in nicaragua. | the four dengue virus (denv) serotypes (denv serotype 1 [denv-1] to denv-4) are transmitted by aedes aegypti and a. albopictus mosquitoes, causing up to 390 million denv infections worldwide each year. we previously reported a clade replacement of the denv-2 asian-american genotype ni-1 clade by the ni-2b clade in managua, nicaragua. here, we describe our studies of the replicative ability of ni-1 and ni-2b viruses in an a. aegypti cell line (aag2) and a. aegypti mosquitoes reared from eggs coll ... | 2014 | 25187539 |
| dengue virus infections among haitian and expatriate non-governmental organization workers--léogane and port-au-prince, haiti, 2012. | in october 2012, the haitian ministry of health and the us cdc were notified of 25 recent dengue cases, confirmed by rapid diagnostic tests (rdts), among non-governmental organization (ngo) workers. we conducted a serosurvey among ngo workers in léogane and port-au-prince to determine the extent of and risk factors for dengue virus infection. of the total 776 staff from targeted ngos in léogane and port-au-prince, 173 (22%; 52 expatriates and 121 haitians) participated. anti-dengue virus (denv) ... | 2014 | 25356592 |
| species composition, seasonal occurrence, habitat preference and altitudinal distribution of malaria and other disease vectors in eastern nepal. | it is increasingly recognized that climate change can alter the geographical distribution of vector-borne diseases (vbds) with shifts of disease vectors to higher altitudes and latitudes. in particular, an increasing risk of malaria and dengue fever epidemics in tropical highlands and temperate regions has been predicted in different climate change scenarios. the aim of this paper is to expand the current knowledge on the seasonal occurrence and altitudinal distribution of malaria and other dise ... | 2014 | 25430654 |
| a database of circadian and diel rhythmic gene expression in the yellow fever mosquito aedes aegypti. | the mosquito species aedes aegypti is the primary vector of many arboviral diseases, including dengue and yellow fevers, that are responsible for a large worldwide health burden. the biological rhythms of mosquitoes regulate many of the physiological processes and behaviors that influence the transmission of these diseases. for insight into the molecular basis of biological rhythms, diel and circadian gene expression profiling has been carried out for many species. to bring these resources to ae ... | 2014 | 25516260 |
| surveillance for dengue and dengue-associated neurologic syndromes in the united states. | autochthonous dengue virus transmission has occurred in the continental united states with increased frequency during the last decade; the principal vector, aedes aegypti, has expanded its geographic distribution in the southern united states. dengue, a potentially fatal arboviral disease, is underreported, and us clinicians encountering patients with acute febrile illness consistent with dengue are likely to not be fully familiar with dengue diagnosis and management. recently, investigators sug ... | 2014 | 25371183 |