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inhibition of multidrug resistant listeria monocytogenes by peptides isolated from combinatorial phage display libraries.the aim of the study was to isolate and characterize novel antimicrobial peptides from peptide phage library with antimicrobial activity against multidrug resistant listeria monocytogenes. combinatorial phage-display library was used to affinity select peptides binding to the cell surface of multidrug resistant l. monocytogenes. after several rounds of affinity selection followed by sequencing, three peptides were revealed as the most promising candidates. peptide l2 exhibited features common to ...201727296960
validation of the ansr(®) listeria monocytogenes method for detection of listeria monocytogenes in selected food and environmental samples.work was conducted to validate performance of the ansr(®) for listeria monocytogenes method in selected food and environmental matrixes. this dna-based assay involves amplification of nucleic acid via an isothermal reaction based on nicking enzyme amplification technology. following single-step sample enrichment for 16-24 h for most matrixes, the assay is completed in 40 min using only simple instrumentation. when 50 distinct strains of l. monocytogenes were tested for inclusivity, 48 produced p ...201726833248
listeria monocytogenes cholecystitis in an immunocompetent patient. 201726826779
linezolid and dexamethasone experience in a serious case of listeria rhombencephalitis.listeria rhombencephalitis is a rare cause of brain stem encephalitis. we report a case with a history of immunosupressive therapy due to takayasu's arteritis that was treated with corticosteroids and linezolid for listeria rhombencephalitis. a 63-year-old woman was admitted to the hospital with fever, headache, nausea, and vomiting. the patient's body temperature was 38°c, and she had a stiff neck. listeria monocytogenes was isolated from the cerebrospinal fluid (csf), and penicillin g and gent ...201726860968
a case report of listeria monocytogenes abscesses presenting as cortically predominant ring-enhancing lesions.listeria monocytogenes, a common cause of bacterial meningitis, rarely involves the central nervous system (cns) in the form of multiple cerebral ring-enhancing lesions.201726034484
development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of listeria monocytogenes.listeria monocytogenes, one of most problematic foodborne pathogens, is responsible for listeriosis in both humans and animals and mainly transmitted through the food chain. in this report, we propose a simple, rapid, and nearly instrument-free molecular technique using multiple cross displacement amplification (mcda) label-based gold nanoparticles lateral flow biosensor (lfb) for specific, sensitive, and visual detection of l. monocytogenes. the mcda-lfb method was carried out at a constant tem ...201728138243
structural characterization and in vitro fermentation of β-mannooligosaccharides produced from locust bean gum by gh-26 endo-β-1,4-mannanase (manb-1601).size exclusion chromatography of β-mannooligosaccharides (β-mos) mixtures, obtained from manb-1601 hydrolysis of locust bean gum, resulted in separation of oligosaccharides with various degrees of polymerization (dp 2, 3, and 5). the oligosaccharides were structurally [esi-ms, ftir, xrd, tga, and nmr ((1)h and (13)c)] and functionally (in vitro fermentation) characterized. dp2 oligosaccharide was composed of two species, (a) mannopyranose β-1,4 mannopyranose and (b) α-1,6-galactosyl-mannopyranos ...201728225615
enrichment, amplification, and sequence-based typing of salmonella enterica and other foodborne pathogens.detection of salmonella enterica in foods typically involves microbiological enrichment, molecular-based assay, and subsequent isolation and identification of a pure culture. this is ideally followed by strain typing, which provides information critical to the investigation of outbreaks and the attribution of their sources. pulsed-field gel electrophoresis is the "gold standard" for s. enterica strain typing, but its limitations have encouraged the search for alternative methods, including whole ...201728221883
microbial performance of food safety control and assurance activities in a fresh produce processing sector measured using a microbial assessment scheme and statistical modeling.current approaches such as inspections, audits, and end product testing cannot detect the distribution and dynamics of microbial contamination. despite the implementation of current food safety management systems, foodborne outbreaks linked to fresh produce continue to be reported. a microbial assessment scheme and statistical modeling were used to systematically assess the microbial performance of core control and assurance activities in five kenyan fresh produce processing and export companies ...201728221882
microbiological quality of pastrami and associated surfaces at the point of sale in kayseri, turkey.the aim of this study is to trace the possible relations between the hygienic status of slicing utensils and the microbiological quality of pastrami.201728216007
the effects of atmospheric pressure cold plasma treatment on microbiological, physical-chemical and sensory characteristics of vacuum packaged beef loin.effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (acp) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. exposure of non-covered beef samples under high power acp conditions resulted in increased a*, b*, chroma and hue values, but acp treatment of packaged loins did not impact colour (l*, a*, b*, chroma, hue), lipid peroxidation, sarcoplasmic protein denaturation, nitr ...201728214695
influence of electro-activated solutions of weak organic acid salts on microbial quality and overall appearance of blueberries during storage.the aim of this work was to study the potential of diluted electro-activated solutions of weak organic acid salts (potassium acetate, potassium citrate and calcium lactate) to extend the shelf life of blueberries during post-harvest storage. the sanitizing capacity of these solutions was studied against pathogenic bacteria listeria monocytogenes and e. coli o157:h7 as well as phytopathogenic fungi a. alternata, f. oxysporum and b. cinerea. the results showed that a 5-min treatment of inoculated ...201728213035
inactivation of salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes in chicken purge or skin using a 405-nm led array.raw poultry are sometimes contaminated with foodborne pathogens, which can lead to illness in humans. in recent years research has focused on a variety of light technologies to decontaminate food and food contact surfaces during meat and poultry processing. in this study we evaluated the ability of 405-nm light generated from an led array to inactivate multi-isolate cocktails of either salmonella spp., pathogenic escherichia coli, staphylococcus spp., or listeria monocytogenes suspended in chick ...201728213017
prevalence of listeria monocytogenes in retail lightly pickled vegetables and its successful control at processing plants.incidences of food poisoning traced to nonanimal food products have been increasingly reported. one of these was a recent large outbreak of shiga toxin-producing escherichia coli (stec) o157 infection from the consumption of lightly pickled vegetables, indicating the necessity of imposing hygienic controls during manufacturing. however, little is known about the bacterial contamination levels in these minimally processed vegetables. here we examined the prevalence of stec, salmonella spp., and l ...201728207308
evaluation of the effect of radiofrequency radiation emitted from wi-fi router and mobile phone simulator on the antibacterial susceptibility of pathogenic bacteria listeria monocytogenes and escherichia coli.mobile phones and wi-fi radiofrequency radiation are among the main sources of the exposure of the general population to radiofrequency electromagnetic fields (rf-emf). previous studies have shown that exposure of microorganisms to rf-emfs can be associated with a wide spectrum of changes ranged from the modified bacterial growth to the alterations of the pattern of antibiotic resistance. our laboratory at the nonionizing department of the ionizing and non-ionizing radiation protection research ...201728203122
attachment of 13 types of foodborne bacteria to jalapeño and serrano peppers and antibacterial effect of roselle calyx extracts, sodium hypochlorite, colloidal silver, and acetic acid against these foodborne bacteria on peppers.chili peppers are a very important crop in mexico. however, these peppers have been associated with salmonella infection outbreaks in the united states, and salmonella and diarrheagenic escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in mexico. to decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. roselle ( h ...201728199144
anti-pathogenic and probiotic attributes of lactobacillus salivarius and lactobacillus plantarum strains isolated from feces of algerian infants and adults.sixty-seven (67) lactic acid bacteria (lab) isolates belonging to lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by maldi-tof spectrometry and 16s rdna sequencing as lactobacillus plantarum (p25lb1 and p98lb1) and lactobacillus salivarius ...201728093322
a study on prevalence of microbial contamination on the surface of raw salad vegetables.the present work evaluates the microbiological quality of raw salad vegetables (rsv) consumed in dhanbad city, india. a total of 480 samples of 8 different raw salad vegetables from local market were examined for overall microbial quality in terms of aerobic mesophilic, psychrotrophic counts, yeast, mould and total coliform levels. e. coli o157:h7, listeria monocytogenes and salmonella sp. were detected by real-time polymerase chain reaction (qpcr) subsequent to isolation. results showed that al ...201728391475
finding novel antibiotic substances from medicinal plants - antimicrobial properties of nigella sativa directed against multidrug-resistant bacteria.the progressive rise in multidrug-resistant (mdr) bacterial strains poses serious problems in the treatment of infectious diseases. while the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. this rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. the medicinal herb nigella sativa and its derivatives constitute promising candidates. ...201728386474
microbiological and sensory quality of fresh ready-to-eat artichoke hearts packaged under modified atmosphere.in recent years the sales of minimally processed vegetables have grown exponentially as a result of changes in consumer habits. the availability of artichoke buds as a ready-to-eat product would be, therefore, highly advantageous. however, minimally processed artichoke hearts are difficult to preserve because of their rapid browning and the proliferation of naturally occurring microorganisms. we developed artichoke hearts prepared as ready-to-eat products that maintain the characteristics of the ...201728358262
disinfection and removal of human pathogenic bacteria in arctic waste stabilization ponds.wastewater stabilization ponds (wsps) are commonly used to treat municipal wastewater in arctic canada. the biological treatment in the wsps is strongly influenced by climatic conditions. currently, there is limited information about the removal of fecal and pathogenic bacteria during the short cool summer treatment season. with relevance to public health, the objectives of this paper were to determine if treatment in arctic wsps resulted in the disinfection (i.e., removal of fecal indicator bac ...201728353112
production of reuterin in a fermented milk product by lactobacillus reuteri: inhibition of pathogens, spoilage microorganisms, and lactic acid bacteria.we assessed the antimicrobial activity of reuterin produced in vitro in glycerol aqueous solutions in situ by lactobacillus reuteri atcc 53608 as part of a fermented milk product against starter (lactobacillus delbrueckii ssp. bulgaricus and streptococcus thermophilus), spoilage (penicillium expansum), pathogenic (staphylococcus aureus, salmonella enterica ssp. enterica, and listeria monocytogenes), and pathogen surrogate (escherichia coli dh5α) microorganisms. we also assayed the influence of c ...201728342608
antibacterial activity of phenyllactic acid against listeria monocytogenes and escherichia coli by dual mechanisms.phenyllactic acid (pla), a phenolic acid phytochemical, is considered to be a promising candidate for use as a chemical preservative due to its broad antimicrobial activity. the antibacterial target of pla has rarely been reported, thus investigations were performed to elucidate the antibacterial mechanism of pla against listeria monocytogenes and escherichia coli. flow cytometry analysis stained with propidium iodide (pi) demonstrated that pla could damage the membrane integrity of l. monocytog ...201728317760
prevalence of foodborne pathogens in food from selected african countries - a meta-analysis.food safety information in the african region is insufficient and fragmented due to lack of surveillance, documentation and reporting, thereby resulting in inefficient utilization of resources, duplication of activities, and lack of synergy among the countries of the region. this paper reviews the prevalence of foodborne pathogens in seven african countries (benin, botswana, ghana, kenya, nigeria, sudan and uganda) from papers in regional or international journals published between january 2000 ...201728271855
facile approach for large-scale production of metal and metal oxide nanoparticles and preparation of antibacterial cotton pads.metallic nanoparticles such as zinc oxide (znonps), copper oxide (cuonps), and silver (agnps) were synthesized in gram scale using green methods. the antibacterial cotton fibers/nanoparticles (cotton fibers/nps) composite pads were prepared, and nanoparticle binding/release tests were performed. all the nps were crystalline and showed characteristic xrd diffraction peaks and showed the characteristic ftir bands of the respective nanoparticles. all the nps showed strong antimicrobial activity aga ...201728267490
one-step preparation of banana powder/silver nanoparticles composite films.silver nanoparticles (ag-nps) were synthesized using banana powder as a reducing and stabilizing agent, and banana/ag-nps composite films with different concentration of ag-nps were prepared simultaneously. the composite films were yellowish brown and exhibited characteristic plasmon resonance peak of ag-nps at 430 nm. the optical, mechanical, water vapor barrier, thermal stability, and antimicrobial properties of the composite films were greatly influenced by the concentration of ag-nps. the co ...201728242949
the effect of preparation processes on the physicochemical characteristics and antibacterial activity of chitooligosaccharides.the bioactivities of chitooligosaccharides are markedly influenced by the degree of acetylation, degree of polymerization or molecular weight and pattern of acetylation. thus, it is crucial to identify reproducible processes that will give rise to well-defined chitooligosaccharides and establish methods for their posterior physicochemical characterization in order to advance in the knowledge of their bioactivity. chitooligosaccharides were prepared by two different processes. the first used chit ...201727987925
preparation and characterisation of chlorogenic acid-gelatin: a type of biologically active film for coating preservation.chlorogenic acid (cga), a type of plant polyphenol, was conjugated onto gelatin (gel) to prepare a novel coating material for the preservation of fresh seafood. the optimal reaction molar ratio of cga to gelatin (4:1) was determined according to the cga content in the cga-gel conjugate. cga was confirmed to be successfully conjugated onto gelatin by (1)h nuclear magnetic resonance and fourier transform-infrared spectroscopy. the antioxidant activity of cga-gel was proven to be higher than that o ...201727979255
modeling stochastic variability in the numbers of surviving salmonella enterica, enterohemorrhagic escherichia coli, and listeria monocytogenes cells at the single-cell level in a desiccated environment.despite effective inactivation procedures, small numbers of bacterial cells may still remain in food samples. the risk that bacteria will survive these procedures has not been estimated precisely because deterministic models cannot be used to describe the uncertain behavior of bacterial populations. we used the poisson distribution as a representative probability distribution to estimate the variability in bacterial numbers during the inactivation process. strains of four serotypes of salmonella ...201727940547
presence of foodborne pathogens, extended-spectrum β-lactamase -producing enterobacteriaceae, and methicillin-resistant staphylococcus aureus in slaughtered reindeer in northern finland and norway.various food-producing animals were recognized in recent years as healthy carriers of bacterial pathogens causing human illness. in northern fennoscandia, the husbandry of semi-domesticated reindeer (rangifer tarandus tarandus) is a traditional livelihood and meat is the main product. this study determined the presence of selected foodborne pathogens, methicillin-resistant staphylococcus aureus (mrsa), and extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in healthy semi-domestic ...201728049493
cord blood acute phase reactants predict early onset neonatal sepsis in preterm infants.early onset sepsis (eos) is a major cause of morbidity and mortality in preterm infants, yet diagnosis remains inadequate resulting in missed cases or prolonged empiric antibiotics with adverse consequences. evaluation of acute phase reactant (apr) biomarkers in umbilical cord blood at birth may improve eos detection in preterm infants with intrauterine infection.201728045978
inhibition of crispr-cas9 with bacteriophage proteins.bacterial crispr-cas systems utilize sequence-specific rna-guided nucleases to defend against bacteriophage infection. as a countermeasure, numerous phages are known that produce proteins to block the function of class 1 crispr-cas systems. however, currently no proteins are known to inhibit the widely used class 2 crispr-cas9 system. to find these inhibitors, we searched cas9-containing bacterial genomes for the co-existence of a crispr spacer and its target, a potential indicator for crispr in ...201728041849
occurrence and characterization of food-borne pathogens isolated from fruit, vegetables and sprouts retailed in the czech republic.food of non-animal origin is a major component of the human diet and has been considered to pose a low risk from the point of view of bacteriological safety. however, an increase in the number of outbreaks of illness caused by such pathogens and linked to the consumption of fresh fruit and vegetables have been reported from around the world recently. salmonella spp., stec (shiga toxin producing escherichia coli) and listeria monocytogenes are among the most frequently identified agents. addition ...201728040162
novel bacteriocinogenic enterococcus hirae and pediococcus pentosaceus strains with antilisterial activity isolated from brazilian artisanal cheese.we isolated and characterized bacteriocin producers enterococcus hirae st57acc and pediococcus pentosaceus st65acc from raw milk artisanal cheeses. their bacteriocins were tolerant to temperatures from 4°c to 100°c and under sterilization conditions (121°c for 15 min). additionally, the tested bacteriocins remained active after being exposed to ph 2.0 to 10.0 for 2 h. the activity of the bacteriocins was affected by proteolytic enzymes but remained stable after treatment with edta, sodium dodecy ...201728189328
influence of pdo ragusano cheese biofilm microbiota on flavour compounds formation.the objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from a to k), producing pdo ragusano cheese, and to investigate on its ability to generate volatile organic compounds (vocs) in milk samples inoculated with biofilm and incubated under ragusano cheese making conditions. the biofilms were subjected to plate counting and pcr/t/dgge analysis and the vocs generated in incubated milk samples were evaluated through smartnose, gc/o, and gc/ms. strepto ...201727697162
effect of iranian ziziphus honey on growth of some foodborne pathogens.honey has previously been shown to have wound healing and antimicrobial properties, but this is dependent on the type of honey, geographical location, and flower from which the final product is derived. we tested the antimicrobial activity of a natural honey originating from the ziziphus spina-christi tree, against selected strains of bacteria. ziziphus honey among more than a 100 verities of honey is known to have the greatest value of energy and minerals in it.201727003970
in vivo and in vitro effects of selected antioxidants on rabbit meat microbiota.the purpose of this study was to investigate the effect of dietary vitamin e or economase™ supplementation on the growth of several background/pathogenic bacteria on rabbit carcasses and hamburgers during refrigerated storage. for 51days, 270 new zealand rabbits received either a basal diet, or experimental diets enriched with 100 or 200mg/kg of vitamin e or economase™. the bacteria studied were salmonella, listeria monocytogenes, pseudomonas, enterobacteriaceae, escherichia coli, coagulase-posi ...201727664938
isolation and partial characterization of 3 nontoxic d-galactose-specific isolectins from seeds of momordica balsamina.three isolectins denoted hereforth mbal-30, mbal-60, and mbal-80 were isolated from seeds extract of momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. the native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kda, respectively. on sds-page, under reduced condition, 27 kda band was obtained for all isolectins. the lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations  ...201727774692
removal of listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments.the effects of pronase (prn), cellulase (cel) or dnasei alone or combined with benzalkonium chloride (bac) against listeria monocytogenes-carrying biofilms were assayed. the best removal activity against l. monocytogenes-escherichia coli biofilms was obtained using dnasei followed by prn and cel. subsequently, a modified logistic model was used to quantify the combined effects of prn or dnasei with bac. a better bac performance after prn compared to dnasei eradicating l. monocytogenes was observ ...201727918204
comparative genomics reveals the diversity of restriction-modification systems and dna methylation sites in listeria monocytogenes.listeria monocytogenes is a bacterial pathogen that is found in a wide variety of anthropogenic and natural environments. genome sequencing technologies are rapidly becoming a powerful tool in facilitating our understanding of how genotype, classification phenotypes, and virulence phenotypes interact to predict the health risks of individual bacterial isolates. currently, 57 closed l. monocytogenes genomes are publicly available, representing three of the four phylogenetic lineages, and they sug ...201727836852
antimicrobial activity of whey protein films supplemented with lactobacillus sakei cell-free supernatant on fresh beef.the aim of this work was to evaluate the antimicrobial activity of whey protein isolate (wpi) films supplemented with lactobacillus sakei nrrl b-1917 cell-free supernatant on beef inoculated with escherichia coli atcc 25922 or listeria monocytogenes scott a; additionally, sensory evaluation was performed on wrapped beef cubes. supernatant concentrates were obtained from lb. sakei cultures in mrs broth after centrifugation, filtering, and freeze-drying. films were prepared with wpi (3% w/w), algi ...201727889150
in vitro safety assessments and antimicrobial activities of lactobacillus rhamnosus strains isolated from a fermented mare's milk.safety and probiotic characteristics such as antimicrobial activities of three lactobacillus rhamnosus strains, fsmm15, fsmm22 and fsmm26, previously isolated as potential probiotics from fermented mare's milk were investigated. the three fsmm strains were susceptible to ampicillin, gentamycin, kanamycin, streptomycin, tetracycline and chloramphenicol, whereas they were resistant to erythromycin (minimal inhibitory concentration (mic) = 4-8 µg/ml) and clindamycin (mic = 4 µg/ml); bioconversion o ...201727476815
a small-scale experiment of using phage-based probiotic dietary supplement for prevention of e. coli traveler's diarrhea.traveler's diarrhea (td) is caused by escherichia coli in 30% of cases. we have developed a phage cocktail for prophylaxis of td caused by e.coli, shigella flexneri, shigella sonnei, salmonella enterica, listeria monocytogenes or staphylococcus aureus, and investigated its effectiveness against infection caused by the non-pathogenic lac (-) strain of e.coli k12 c600 in animal and human trials. on the 6th day of both animal and human trials e. coli k12 c600 strain was detected in titer of 10(4) c ...201726458758
invasive listeriosis in a patient with several episodes of antibiotic associated colitis presumably due to clostridium difficile.a 62-year-old man developed a blood stream infection and meningitis due to listeria monocytogenes, 20 days after an episode of pseudo-membranous colitis. the patient, hospitalized for the first time for transurethral prostatectomy, was readmitted 20 days later with watery diarrhea. pseudo-membranous colitis was diagnosed and treated successfully, without testing for clostridium difficile infection (cdi). after 15 more days, the patient developed again diarrhea, fever and confusion. hospitalized ...201728365900
galleria mellonella as an in vivo model for assessing the protective activity of probiotics against gastrointestinal bacterial pathogens.the antagonistic activity against gastrointestinal bacterial pathogens is an important property of probiotic bacteria and a desirable feature for pre-selection of novel strains with probiotic potential. pre-screening of candidate probiotics for antibacterial activity should be based on in vitro and in vivo tests. this study investigated whether the protective activity of probiotic bacteria against gastrointestinal bacterial pathogens can be evaluated using galleria mellonella larvae as an in viv ...201728369512
seeds of the wild progenitor of maize possess bacteria that antagonize foodborne pathogens.endophytes are microorganisms that inhabit plant tissues without causing disease. some endophytes help their hosts to combat pathogens. here we explored the hypothesis that the plant-derived foods consumed by humans and other animals host endophytes that also antagonize foodborne pathogens or food-rotting agents. our laboratory previously cultured a library of bacterial endophytes from different members of the maize/corn family (zea) including wild relatives. here, 190 of these endophytes were s ...201728186422
the microbiota of marketed processed edible insects as revealed by high-throughput sequencing.entomophagy has been linked to nutritional, economic, social and ecological benefits. however, scientific studies on the potential safety risks in eating edible insects need to be carried out for legislators, markets and consumers. in this context, the microbiota of edible insects deserves to be deeply investigated. the aim of this study was to elucidate the microbial species occurring in some processed marketed edible insects, namely powdered small crickets, whole dried small crickets (acheta d ...201727889142
enterocin b3a-b3b produced by lab collected from infant faeces: potential utilization in the food industry for listeria monocytogenes biofilm management.enterococcus faecalis b3a-b3b produces the bacteriocin b3a-b3b with activity against listeria monocytogenes, staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa) and clostridium perfringens, but apparently not against fungi or gram-negative bacteria, except for salmonella newport. b3a-b3b enterocin has two different nucleotides but similar amino acid composition to the class iib mr10a-mr10b enterocin. b3a-b3b consists of two peptides of predicted molecular mass of 5176.31 da ...201727878401
improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria.tropical shrimp is of considerable economic importance in the world but is highly perishable due to microbial and chemical degradation. biopreservation is a food preservation technology based on the addition of "positive" bacteria able to kill or prevent the growth of undesirable microorganisms. two strains of lactic acid bacteria (lab) have previously been selected for a biopreservation strategy: lactococcus piscium cncm i-4031, for its ability to prevent the sensory deterioration of seafood an ...201727760400
human α-defensin 6: a small peptide that self-assembles and protects the host by entangling microbes.human α-defensin 6 (hd6) is a 32-residue cysteine-rich peptide that contributes to innate immunity by protecting the host at mucosal sites. this peptide is produced in small intestinal paneth cells, stored as an 81-residue precursor peptide named prohd6 in granules, and released into the lumen. one unusual feature of hd6 is that it lacks the broad-spectrum antimicrobial activity observed for other human α-defensins, including the paneth cell peptide human α-defensin 5 (hd5). hd6 exhibits unprece ...201728296382
essential oils against pathogen and spoilage microorganisms of fruit juices: use of versatile antimicrobial delivery systems.essential oils (eo) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. limonene (0 to 10 μl/ml) was added to microbial suspensions (∼10(5) cfu/ml) of selected foodborne pathogens (listeria monocytogenes scott a, salmonella enterica typhimurium, escherichia coli and staphylococcus aureus), and spoilage microorganisms (lactobacillus plantarum, saccharomyces cerevisiae, and candida albi ...201728071802
antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases.ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ...201726377624
primary pyogenic ventriculitis caused by neisseria meningitidis: case report and review of the literature.background. pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. primary pyogenic ventriculitis is a rare entity and few cases have been described so far. we report the first case of primary pyogenic ventriculitis in an adult caused by neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the english literature. methods. a pubmed search was performed using the terms ependymitis, vent ...201728348798
beneficial and safety properties of a corynebacterium vitaeruminis strain isolated from the cow rumen.corynebacterium vitaeruminis mru4 was isolated from the cow rumen and was differentiated from other isolates by rep-pcr and rapd and identified by 16s rrna sequencing. this strain presented higher survival rates for low ph and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. c. vitaeruminis mru4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with listeria monocytogenes scott a, 41.6% co-aggregation rate with enterococc ...201728258546
diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model.food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ...201728202007
synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers.a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ...201728157594
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
inhibition of listeria monocytogenes on ready-to-eat meats using bacteriocin mixtures based on mode-of-action.bacteriocin-producing (bac⁺) lactic acid bacteria (lab) comprising selected strains of lactobacillus curvatus, lactococcus lactis, pediococcus acidilactici, and enterococcus faecium and thailandicus were examined for inhibition of listeria monocytogenes during hotdog challenge studies. the bac⁺ strains, or their cell-free supernatants (cfs), were grouped according to mode-of-action (moa) as determined from prior studies. making a mixture of as many moas as possible is a practical way to obtain a ...201728335414
determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products.functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ...201728223974
enhanced control of listeria monocytogenes by enterococcus faecium ke82, a multiple enterocin-producing strain, in different milk environments.enterococcus faecium ke82, isolated from traditional greek graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural enta, entb, and entp enterocin genes. e. faecium ke82 was further assessed for in situ antilisterial activity in raw milk (rm) and commercially thermized milk (tm; 63°c for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (srm; 121°c for 5 min) with or without the addition ...201728221890
growth interactions and antilisterial effects of the bacteriocinogenic lactococcus lactis subsp. cremoris m104 and enterococcus faecium ke82 strains in thermized milk in the presence or absence of a commercial starter culture.traditional greek cheeses are often produced from thermized milk (tm) with the use of commercial starter cultures (cscs), which may not inhibit growth of listeria monocytogenes completely. therefore, this study evaluated the behavior of an artificial l. monocytogenes contamination in commercially tm (63 °c; 30 s) inoculated with a csc plus lactococcus lactis subsp. lactis m104 and/or enterococcus faecium ke82, two indigenous strains producing nisin a and enterocin a and b, respectively. inoculat ...201728213019
characterization of class iia bacteriocin resistance in enterococcus faecium.vancomycin-resistant enterococci, particularly resistant enterococcus faecium, pose an escalating threat in nosocomial environments because of their innate resistance to many antibiotics, including vancomycin, a treatment of last resort. many class iia bacteriocins strongly target these enterococci and may offer a potential alternative for the management of this pathogen. however, e. faecium's resistance to these peptides remains relatively uncharacterized. here, we explored the development of r ...201728115354
microbiology of processed edible insect products - results of a preliminary survey.little is known of the microbiology of processed insect products. the present survey analysed a total of n=38 samples of deep-fried and spiced (acheta domesticus, locusta migratoria, and omphisa fuscidentalis), cooked in soy sauce ("tsukudani"; oxya yezoensis, vespula flaviceps, and bombyx mori), dried (a. domesticus, l. migatoria, alphitobius diaperinus, tenebrio molitor, b. mori, hermetia illucens, and musca domestica), powdered (h. illucens, t. molitor) and other (incl. deep-frozen b. mori an ...201727903420
microbiology of cooked and dried edible mediterranean field crickets (gryllus bimaculatus) and superworms (zophobas atratus) submitted to four different heating treatments.to increase the shelf life of edible insects, modern techniques (e.g. freeze-drying) add to the traditional methods (degutting, boiling, sun-drying or roasting). however, microorganisms become inactivated rather than being killed, and when rehydrated, many return to vegetative stadia. crickets (gryllus bimaculatus) and superworms (zophobas atratus) were submitted to four different drying techniques (t1 = 10' cooking, 24 h drying at 60℃; t2 = 10' cooking, 24 h drying at 80℃; t3 = 30' cooking, 12  ...201727235993
strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion.one of the working mechanisms of probiotic bacteria is their ability to compete with pathogens. to define the probiotic properties of seven lactic acid bacteria (lab) strains, we tested them for survival in simulated gastro-intestinal conditions, antimicrobial activities, co-aggregative abilities, and interferences studies against five human intestinal pathogens (salmonella enteritidis atcc 13076, listeria monocytogenes atcc 7644, escherichia coli o157: h7 atcc 35150, cronobacter sakazakii atcc ...201728286570
mutual growth-promoting effect between bifidobacterium bifidum wbbi03 and listeria monocytogenes cmcc 54001.in this paper, bifidobacterium bifidum wbbi03 and listeria monocytogenes cmcc 54001 were selected to detect the changes in their growth pattern after mutual interaction between them. the proteomic changes after the interaction between the 2 bacteria were detected by the isobaric tags for relative and absolute quantitation method. the proteins related to the biosynthesis and cell reproduction were selected, and their changes at the transcriptional level were monitored by fluorescent quantitative ...201728259400
a comparative analysis of the lyve-set phylogenomics pipeline for genomic epidemiology of foodborne pathogens.modern epidemiology of foodborne bacterial pathogens in industrialized countries relies increasingly on whole genome sequencing (wgs) techniques. as opposed to profiling techniques such as pulsed-field gel electrophoresis, wgs requires a variety of computational methods. since 2013, united states agencies responsible for food safety including the cdc, fda, and usda, have been performing whole-genome sequencing (wgs) on all listeria monocytogenes found in clinical, food, and environmental samples ...201728348549
an in vivo reporter assay for srna-directed gene control in gram-positive bacteria: identifying a novel srna target in staphylococcus aureus.bacterial small regulatory rnas (srnas) play a major role in the regulation of various cellular functions. most srnas interact with mrna targets via an antisense mechanism, modifying their translation and/or degradation. despite considerable progresses in discovering srnas in gram-positive bacteria, their functions, for the most part, are unknown. this is mainly due to difficulties in identifying their targets. to aid in the identification of srna targets in gram-positive bacteria, we set up an ...201728369640
biocontrol of the internalization of salmonella enterica and enterohaemorrhagic escherichia coli in mung bean sprouts with an endophytic bacillus subtilis.internalization of salmonella enterica and enterohaemorrhagic escherichia coli (ehec) in seed sprouts poses a health risk to consumers, and the conventional sanitization methods are not always effective to reduce this risk. this study initiated a biocontrol approach to limit the internalization using endophytic bacillus subtilis strains, which were isolated from the inner tissue of mung bean seeds or lettuce stems. by using the deferred agar method, 12 strains of b. subtilis out of 94 putative b ...201728364624
a redox-responsive transcription factor is critical for pathogenesis and aerobic growth of listeria monocytogenes.bacterial pathogens have evolved sophisticated mechanisms to sense and adapt to redox stress in nature and within the host. however, deciphering the redox environment encountered by intracellular pathogens in the mammalian cytosol is challenging and remains poorly understood. in this study, we assessed the contributions of the two redox-responsive, spx-family transcriptional regulators to the virulence of listeria monocytogenes, a gram-positive facultative intracellular pathogen. spx-family prot ...201728193635
antibacterial and antivirulence effect of 6-n-hydroxylaminopurine in listeria monocytogenes.the emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. in this work we examined the putative antibacterial effect of purine analogs in listeria monocytogenes we show that, among several tested purine analogs, only 6-n-hydroxylaminopurine (6-n-hap) reduces the viability of the gram-positive pathogen listeria monocytogenes as in bacillus subtilis, 6-n-hap terminates expression at guanine riboswitches in l. monocytogenes hence preventing expression ...201728062853
subtilosin prevents biofilm formation by inhibiting bacterial quorum sensing.subtilosin, the cyclic lantibiotic protein produced by bacillus subtilis katmira1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. in this study, subtilosin was purified using ammonium sulfate ((nh4)2so4) precipitation and purified via column chromatography. subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (mic and sub-mic) and anti-biofilm activity (biofilm prevention) were established. subtilosin was evaluated as a quorum ...201727914001
inhibition of listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate.this study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by listeria monocytogenes or to remove existing biofilms in mushroom production facilities.201727797439
raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets.microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ...201727747902
inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination.the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ...201728040158
antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs.crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal.201727925492
antibiotic discovery throughout the small world initiative: a molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity.the emergence of bacterial pathogens resistant to all known antibiotics is a global health crisis. adding to this problem is that major pharmaceutical companies have shifted away from antibiotic discovery due to low profitability. as a result, the pipeline of new antibiotics is essentially dry and many bacteria now resist the effects of most commonly used drugs. to address this global health concern, citizen science through the small world initiative (swi) was formed in 2012. as part of swi, stu ...201728110506
cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current.the aim of the study was to investigate the effect of using direct electric current (dc) of 0, 200, and 400 ma for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. the ph, oxidation-reduction potential (orp), electrical conductivity (ec), and available chlorine concentration (acc) were measured. the effect of sodium alginate hydrosols treated with dc on staphylococcus a ...201728327520
biofilm inhibition formation of clinical strains of pseudomonas aeruginosa mutans, photocatalytic activity of azo dye and gc-ms analysis of leaves of lagerstroemia speciosa.the investigation was conducted to analyse the bioactive compounds from the leaf extracts of l. speciosa by gc-ms. the extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. the bioactive compounds from the leaves of l. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by gas chromatography-mass spectroscopy (gc-ms). the antibacterial activity was evaluated against clinical strain like stap ...201728319869
antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects.silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ...201728261161
an assessment of the microbiological quality of liver-based pâté in england 2012-13: comparison of samples collected at retail and from catering businesses.the purpose of this study was to investigate the microbiological quality of liver pâté. during 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect salmonella spp. or campylobacter spp., and to enumerate for listeria spp., including listeria monocytogenes, clostridium perfringens, coagulase-positive staphylococci including ...201728190406
the role of universal stress proteins in bacterial infections.universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ...201728120710
antimicrobial activity, ame resistance, and a-site binding studies of anthraquinone-neomycin conjugates.the antibacterial effects of aminoglycosides are based on their association with the a-site of bacterial rrna and interference with the translational process in the bacterial cell, causing cell death. the clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12s rrna and its deafness-associated mutations, c1494u and a1555g. we report a rapid assay that allows screening of aminoglycoside compounds to ...201728103015
antimicrobial efficacy and safety of mucoadhesive exopolymer produced by acinetobacter haemolyticus.this study evaluated five extracellular polymers of bacterial origin possessing mucoadhesive properties for their antimicrobial properties and toxicological characteristics. of the five tested mucoadhesive biopolymers, the extracellular polymer produced by a strain of acinetobacter haemolyticus exhibited broad antimicrobial efficacy towards yersinia enterocolitica, salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7 and bacillus subtilis. significant (p<0.05) inhibition of g ...201727720965
bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides.several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ...201728197131
tid: standalone software for mining putative drug targets from bacterial proteome.tid is a standalone application, which relies on basic assumption that a protein must be essential for pathogens survival and non-homologous with host to qualify as putative target. with an input bacterial proteome, tid removes paralogous proteins, picks essential ones, and excludes proteins homologous with host organisms. the targets illustrate non-homology with at least 40 out of 84 gut microbes, considered safe for human. tid classifies proposed targets as known, novel and virulent. users can ...201727856224
differential roles of caspase-1 and caspase-11 in infection and inflammation.caspase-1, also known as interleukin-1β (il-1β)-converting enzyme (ice), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. on activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-il-1β and pro-il-18 into their biologically active forms. "ice(-/-)" or "casp1(-/-)" mice generated using 129 embryonic stem cells carry a 129-associated inactivating passenger mutation on the caspase-11 locus, essentially making t ...201728345580
a study of the potential release of bioaerosols from containers as a result of reduced frequency residual waste collections.microorganisms have the potential to grow within waste containers if waste is stored for longer periods as a result of an extended residual waste collection cycle. release of microorganisms as bioaerosols during waste collection and processing may be an occupational risk to workers within the industry. there may be many constituents of the bioaerosol that may be of concern, however, there are currently only workplace exposure limits proposed for endotoxin (90eum(-3)). a field-scale trial was est ...201727810739
active edible coating and γ-irradiation as cold combined treatments to assure the safety of broccoli florets (brassica oleracea l.).the antimicrobial activity of essential oils (eos), organic acid (oa) salts and natamycin, a natural antifungal produced during fermentation by the bacterium streptomyces natalensis, was assessed against four pathogens (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium and aspergillus niger). the minimum inhibitory concentration (mic) of each antimicrobial (am) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. the ...201727744210
isolation and characterisation of an enterocin p-producing enterococcus lactis strain from a fresh shrimp (penaeus vannamei).screening for lactic acid bacteria (lab) from fresh shrimp samples (penaeus vannamei) collected from retail seafood markets in the tunisian's coast, resulted in the isolation of an enterococcus strain termed q1. this strain was selected for its antagonistic activity against pathogenic bacteria such as listeria monocytogenes, pseudomonas aeruginosa, lactococcus garvieae and against fungi (aspergillus niger and fusarium equiseti). the q1 strain was characterised using standard morphological and bi ...201728265787
flexible thin-layer plasma inactivation of bacteria and mold survival in beef jerky packaging and its effects on the meat's physicochemical properties.the aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. after plasma treatment for 10min, escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3log cfu/g. no significant changes in metmyoglobin content, shear force, and myof ...201727718408
sumoylation as an integral mechanism in bacterial infection and disease progression.post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. among these, particularly the modification with small ubiquitin-like modifiers (sumo) is being recognized as a pathway crucial for cell homeostasis and health. understandably, bacterial pathogens intervene with the sumo pathway of the host for ensuring successful infection. among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. majo ...201728197924
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