Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ethanol increases agonist affinity for nicotinic receptors from torpedo. | the presence of ethanol increases the apparent affinity with which acetylcholine and carbamylcholine elicit 86rb+ flux from torpedo nicotinic acetylcholine receptor-rich vesicles at 4 degrees c. affinity increased exponentially with ethanol concentration, reaching nearly 200-fold by 3.0 m ethanol without sign of saturation. at submaximal agonist concentrations 50-100 mm ethanol enhanced flux by 15-35%, but the maximum agonist-induced flux was unaffected in quenched-flow assays. the effect was in ... | 2008 | 2597688 |
| [the dynamic characteristics of regional muscle blood flow and the activity of mineralization processes in the bony callus with various methods of treating mandibular fractures]. | rmbf was comparatively assessed in patients with lower jaw fractures under orthopedic therapy of the osteal suture. to this end, the 99m pertechnetate was injected bilaterally in masseter muscles. the data suggest that double jaw fixation had an adverse effect on rmbf, especially ipsilateral to the fracture. nevertheless, the mineral metabolism intensity in the callus is higher under fixation as related to the cases surgically sutured. | 2008 | 2555937 |
| [persistent mullerian duct syndrome]. | we report on a 14-year-old boy who was admitted to the emergency department for acute retention of urine. at age 1 year, he had been submitted to surgery for left cryptorchidism and left-sided herniation, associated with hypospadias of the glans penis. radiologic and endoscopic work up revealed a large retrovesical cavity with septation in its upper portion communicating with the prostatic urethra. these structures were removed and pathologic examination disclosed remnants of müllerian ducts. th ... | 2008 | 2573317 |
| the inferior gluteal free flap in breast reconstruction. | the inferior gluteal musculocutaneous free flap usually provides a sufficient amount of autogenous tissue for breast reconstruction when adequate tissue is not present in the lower abdomen or back. its arteriovenous pedicle is longer than the superior gluteal musculocutaneous free-flap pedicle and permits microvascular anastomosis in the axilla, avoiding medial rib and cartilage resection. in the thin patient, there is more available donor tissue than with the superior gluteal musculocutaneous f ... | 2008 | 2587651 |
| effect of cepharanthin on superoxide anion (o2-) production by macrophages. | 2008 | 2848880 | |
| [the correct use of beta 2 stimulants]. | 2008 | 2566197 | |
| modulation of a 190-kd microtubule-associated protein in pigment epithelium by vip. | vasoactive intestinal peptide stimulates phosphorylation of six high molecular weight cytosolic proteins in the cultured retinal pigment epithelium (rpe). of these, the 190-kd phosphoprotein is associated with the microtubules assembled by taxol/gtp and is immunologically related to the brain microtubule-associated protein 2 (mol.wt. = 280 kd). vip is also shown here to stimulate secretion in the cultured rpe. vip-stimulated phosphorylation of a 190-kd microtubule-associated protein is also demo ... | 2008 | 2575248 |
| interaction between peptide growth factors and homoeobox genes in the establishment of antero-posterior polarity in frog embryos. | the expression of the xenopus homoeobox gene xhox3 is an early response to mesoderm induction by peptide growth factors and the level of xhox3 expression marks the antero-posterior character of the induced mesoderm. different peptide growth factors specify different antero-posterior mesodermal cell fates as seen by the level of xhox3 expression and the capacity to induce specific secondary neural/epidermal structures. these factors and homoeobox genes thus form part of the mechanism necessary fo ... | 2008 | 2570357 |
| the effects of h1 antihistamines on the early allergic response. | to increase understanding of the effect of h1 antihistamines on the immediate response to nasal challenge with antigen, we performed two double blind, placebo-controlled, crossover studies using cetirizine and terfenadine. the subjects underwent nasal challenge with antigen after premedication with either cetirizine (20 mg qd for two days, n = 10), terfenadine (60 mg bid for 1 week, n = 12), or placebo for equivalent periods of time. we monitored the response to challenge by counting the number ... | 2008 | 2574550 |
| use-dependent block of sodium channels in frog myelinated nerve by tetrodotoxin and saxitoxin at negative holding potentials. | na+ currents were measured in myelinated frog nerve fibres in the presence of nanomolar concentrations of tetrodotoxin (ttx) or saxitoxin (stx) in the extracellular solution. the na+ currents declined during a train of depolarizing pulses if the fibre was held at hyperpolarizing potentials between the pulses. at a pulse frequency of 0.8 hz, the peak na+ currents were reduced to 70 or 60% of the initial value in 9.3 nm ttx and 3.5 nm stx solutions, respectively. a decline of na+ currents was also ... | 2008 | 2553115 |
| [can flame silanization change dental alloy joints?]. | 2008 | 2700822 | |
| salivary testosterone levels and the progress of puberty in the normal boy. | salivary testosterone (st) levels were measured in 84 boys aged 7.3-16.2 from the edinburgh growth study. the correlation coefficient between matched plasma/saliva samples was 0.88. six samples were collected over the course of one day from 0900 to 2100 h each month in the majority of the children for 4 consecutive months. mean daily st levels showed a significant rise between each pubertal stage (genital (g) and pubic hair (ph]. the rise in st became more rapid once a mean testicular volume (mt ... | 2008 | 2605791 |
| choledochal cysts in the adult. | although choledochal cyst is generally considered a disorder of childhood, in approximately 20% of patients a cyst is first recognized in adult life. we reviewed nine adults who underwent operation for choledochal cyst from 1974-1985 at yale new haven hospital. presenting symptoms included: crampy right upper quadrant pain, jaundice, pruritus, cholangitis, pancreatitis, hepatomegaly, and elevated liver function tests. seven patients had undergone previous biliary surgery. the eight patients with ... | 2008 | 2980768 |
| is etoposide injection a vesicant? | 2008 | 2791480 | |
| is etoposide injection a vesicant? | 2008 | 2791480 | |
| contact dermatitis. | 2008 | 3527125 | |
| building competence in delayed and disturbed preschoolers: outcome evaluation of an intensive day treatment program. | 2008 | 2427178 | |
| [search for a marker substance of cinnamomi cortex leading to quality evaluation of a chinese-medicinal formulation "gorei-san"]. | 2008 | 3449174 | |
| the disconnected visual system mutations in drosophila melanogaster drastically disrupt circadian rhythms. | mutations at the disconnected (disco) locus in drosophila melanogaster cause cultures of this insect to eclose in an essentially arrhythmic manner and also nearly eliminate free-running circadian rhythms of locomotor activity. yet disco mutants are not totally light-insensitive: whereas they performed very poorly in tests of certain behavioral responses to visual stimuli, they were able to exhibit "forced" periodic locomotor activity under conditions of light-dark cycling. we discuss these resul ... | 2008 | 2519577 |
| [improving the tactics in the preoperative care and surgical technics for patients with pulmonary tuberculosis and diseases of the liver]. | on the basis of the analysis of the results of surgical treatment of 114 patients, the authors show that in liver pathology, continued aggravation of destructive tuberculosis occurs. to reduce the risk of operation, it is suggested to perform intensive preoperative preparation with inclusion of hemosorption and laser irradiation. | 2008 | 2615200 |
| locked scapula: intrathoracic dislocation of the inferior angle. a case report. | 2008 | 4651271 | |
| the speech of a postlingually deafened teenager during the first year of use of a multichannel cochlear implant. | the speech of a profoundly postlingually deafened teenager was recorded before, immediately after, 3 months after, and 1 year after electrical stimulation with a nucleus multichannel cochlear implant. listener tests of target words revealed significant improvement in overall quality over the year. spectrograms showed less aspiration and better definition of the lower formants. acoustic measurements indicated immediate change in f0 and gradual changes in syllable duration and some aspects of voic ... | 2008 | 2600301 |
| the potential value of the demonstration of thyroglobulin by immunoperoxidase techniques in fine needle aspiration cytology. | the peroxidase-antiperoxidase method for the demonstration of thyroglobulin was performed on 30 fine needle aspiration smears. all of the benign thyroid lesions plus the papillary and follicular carcinomas of the thyroid were positive for thyroglobulin. two cases of metastatic thyroid carcinoma in lymph nodes were also positive. medullary carcinoma of the thyroid and ten control smears from various other tissues were negative. this proves the sensitivity and the specificity of the method, which ... | 2008 | 2447717 |
| genetics and fetal hydantoin syndrome. | 2008 | 2493186 | |
| prospectively randomized clinical trial of three intensive chemotherapy regimens for the treatment of advanced unfavorable histology non-hodgkin's lymphoma. | three hundred thirty-two eligible patients with advanced (ann arbor stage iii or iv) non-hodgkin's lymphoma of aggressive histologic subtype (rappaport classification diffuse histiocytic [dh], diffuse poorly differentiated lymphocytic [dpdl], diffuse mixed [dm], or diffuse undifferentiated [du]) were randomly assigned to receive induction chemotherapy with one of three intensive regimens in a clinical trial conducted by the eastern cooperative oncology group (ecog) between 1978 and 1983. chemoth ... | 2008 | 2442322 |
| who benefits most from respite care? | this study examines the effect of five measures of utilization of respite care services (desire to use respite care services, actual use, accessibility, scheduling and helpfulness) upon mothers' coping resources. using the analysis of variance between utilization measures and coping resources with the following intervening variables as covariates--mothers' self-esteem, family cohesion and adaptation, and clients' and mothers' characteristics--it was found that respite care acts as a differential ... | 2008 | 2479611 |
| a non-enzymatic method for identification of citrullinemia heterozygotes. | 2008 | 2736797 | |
| abnormalities of basophil "releasability" in atopic and asthmatic individuals. | 2008 | 2431023 | |
| stress, locus of control, social support, and psychological symptoms among head nurses. | this study was conducted to evaluate the relationships among work stress, nonwork stress, locus of control, social support, and head nurses' psychological symptoms. data were collected from head nurses (n = 300) representing all army hospitals in the united states; 21% of the volunteer sample were men. the results supported the hypotheses that perceived stress from both work and nonwork sources was positively related to psychological symptoms. direct effects for both internal locus of control an ... | 2008 | 2270304 |
| nonneoplastic hematopoietic myeloproliferative syndrome induced by dysregulated multi-csf (il-3) expression. | post 5-fluorouracil-treated murine marrow cells were infected with a retroviral vector (mpzen) bearing a multi-potential colony stimulating factor (multi-csf) cdna insert and then transplanted into lethally irradiated syngeneic recipients to study the effects of autocrine production of multi-csf in normal hematopoietic cells. extremely high levels (14,000 u/ml) of multi-csf were detected in the sera and in media conditioned by various hematopoietic tissues of the transplanted animals. while sple ... | 2008 | 2653457 |
| factors influencing the utilization of reduced nicotinamide adenine dinucleotide by pigeon heart mitochondria. | 2008 | 4287409 | |
| [use of pit and fissure sealants in private practice: the quebec situation]. | this paper presents data collected in march of 1989 from a sample of dentists practicing in the province of quebec. a survey was mailed to dentists in order to determine to what degree sealants are used and the various characteristics relative to the dentists in question. other questions related to how dentists obtained information about sealants, the difficulties encountered with their application and the problems relative to their use by the dental profession. the results indicate that nearly ... | 2008 | 2394870 |
| [neuro-anatomy of retinoblastoma]. | in four cases of retinoblastoma examined with the method of golgi we have only found vessels and glial elements at different stages of differentiation. moreover, in the areas containing typical rosettes, golgi method revealed some isolately impregnated elements resembling photoreceptors in evolution. within the retina abuting on the tumor were found only normal retinal nervous elements. some nervous elements were found also in areas where the tumor tissue intermingled with infiltrated neighbouri ... | 2008 | 2258552 |
| no room at the trough: an epitaph for the eighties. | 2008 | 2217593 | |
| [performing lympho-venous anastomoses at several levels of the lower extremity in primary lymphedema]. | operations were conducted on 41 patients, 31 of them had a primary form of lymphedema. lymphovenous anastomoses (lva) were formed in the region of the medial collector on the thigh in scarpa's triangle, in the middle third of the leg, and on the dorsum of the foot. the results were good in the late-term postoperative period in 74.0% of patients. according to earlier studies, the formation of lva in patients with primary lymphedema of the lower limbs only on one of the standard levels produces go ... | 2008 | 2292856 |
| long-acting somatostatin analog controls acid and gastrin secretion in benign, not in malignant, zollinger-ellison syndrome. | the long-acting somatostatin (sms) analog, sms 201-995 has beneficial effects on apudomas. in two zollinger-ellison syndrome (zes) patients we assessed basal acid output (bao) and 24-h ph under sms and compared them to controls. we also assessed total gastrin, gastrin 17, insulin, glucagon, c-peptide, and sms by radioimmunoassay. in the benign gastrinoma, an acid-controlling action of sms was shown, elevating the 24-h ph threshold over the ph range 1.5-5 of 55-10% compared with control. a parall ... | 2008 | 2754214 |
| a case of o1 vibrio cholera bacteremia and primary peritonitis in a patient with liver cirrhosis. | vibrio cholerae are gram-negative bacteria that are differentiated into o1/o139 and non-o1/non-o139 serogroups depending on their ability to agglutinate with specific antiserum. in contrast to non-o1/non-0139 vibrio cholerae, which are more prone to invade the bloodstream, vibrio cholerae o1 is rarely the cause of bacteremia. we report a case of o1 vibrio cholera bacteremia and primary peritonitis in a patient with liver cirrhosis. the literature on the subject is also reviewed. | 2009 | 27990208 |
| characterization of vibrio cholerae from deep ground water in a cholera endemic area in central india. | a total of 8 out of 11 deep ground water samples collected from different villages in central india were found contaminated with vibrio cholerae non o1, non o139. in a multiplex pcr, isolates were found positive for ompw gene but negative for ctxab and rfbo1 genes. however, isolates from two places were positive for tcp and zot genes, indicating their intestinal colonization and toxigenic potential. antibiotic susceptibility studies revealed that all isolates were multidrug resistant. although, ... | 2009 | 23100781 |
| antimicrobial susceptibility and molecular characterization of vibrio cholerae from cholera outbreaks in chennai. | the genotype and antibiotic resistance pattern of the toxigenic vibrio cholerae strains associated with cholera outbreaks vary frequently. fifty-one v. cholerae strains isolated from cholera outbreaks in chennai (2002-2005) were screened for the presence of virulence and regulatory genes by multiplex polymerase chain reaction (pcr) assay. genotyping of the isolates was done by vc1 primers derived from enterobacterial repetitive intergenic consensus (eric)-related sequence in v. cholerae. all the ... | 2009 | 23100755 |
| investigation towards bivalent chemically defined glycoconjugate immunogens prepared from acid-detoxified lipopolysaccharide of vibrio cholerae o1, serotype inaba. | a free amino group present on the acid-detoxified lipopolysaccharide (pmlps) of v. cholerae o1 serotype inaba was investigated for site-specific conjugation. chemoselective pmlps biotinylation afforded the corresponding mono-functionalized derivative, which retained antigenicity. thus, pmlps was bound to carrier proteins using thioether conjugation chemistry. induction of an anti-lps antibody (ab) response in balb/c mice was observed for all conjugates. interestingly, the sera had vibriocidal ac ... | 2009 | 18648931 |
| abundance of sewage-pollution indicator and human pathogenic bacteria in a tropical estuarine complex. | studies on abundance and types of various pollution indicator bacterial populations from tropical estuaries are rare. this study was aimed to estimate current levels of pollution indicator as well as many groups of human pathogenic bacteria and their seasonal variations in different locations in mandovi and zuari rivers in the central west coast of india. the sampling covered the estuarine and upstream regions of these rivers representing premonsoon (may 2005), monsoon (september 2006) and post- ... | 2009 | 18633722 |
| clonal relationship among vibrio cholerae o1 el tor strains isolated in somalia. | one hundred and three vibrio cholerae o1 strains, selected to represent the cholera outbreaks which occurred in somalia in 1998-1999, were characterized by random amplified polymorphic dna patterns, ribotyping, and antimicrobial susceptibility. all strains showed a unique amplified dna pattern and 2 closely related ribotypes (b5a and b8a), among which b5a was the more frequently identified. ninety-one strains were resistant to ampicillin, chloramphenicol, spectinomycin, streptomycin, sulfamethox ... | 2009 | 18774337 |
| bactericidal effect of lactoferrin and lactoferrin chimera against halophilic vibrio parahaemolyticus. | infections caused by vibrio parahaemolyticus, an halophilic member of the genus vibrio, have increased globally in the last 5 years. diarrhea caused by v. parahaemolyticus results from eating raw or undercooked seafood. the aim of this work was to investigate whether lactoferrin and some lactoferrin-peptides have bactericidal activity against vibrio parahaemolyticus atcc 17802, the pandemic strain o3:k6, and the multidrug resistant isolate 727, as well as against vibrio cholerae strains o1 and n ... | 2009 | 18625283 |
| the vibrio cholerae mrp system: cation/proton antiport properties and enhancement of bile salt resistance in a heterologous host. | the mrp operon from vibrio cholerae encoding a putative multisubunit na(+)/h(+) antiporter was cloned and functionally expressed in the antiporter-deficient strain of escherichia coli ep432. cells of ep432 expressing vc-mrp exhibited resistance to na(+) and li(+) as well as to natural bile salts such as sodium cholate and taurocholate. when assayed in everted membrane vesicles of the e. coli ep432 host, vc-mrp had sufficiently high antiport activity to facilitate the first extensive analysis of ... | 2009 | 18311075 |
| identification of an iron-sulfur cluster that modulates the enzymatic activity in nare, a neisseria meningitidis adp-ribosyltransferase. | in prokaryotes, mono-adp-ribose transfer enzymes represent a family of exotoxins that display activity in a variety of bacterial pathogens responsible for causing disease in plants and animals, including those affecting mankind, such as diphtheria, cholera, and whooping cough. we report here that nare, a putative adp-ribosylating toxin previously identified from neisseria meningitidis, which shares structural homologies with escherichia coli heat labile enterotoxin and toxin from vibrio cholerae ... | 2009 | 19744927 |
| identification and characterization of phage ps166 lysogens from non-o1, o139 strains of vibrio cholerae. | in recent years, non-o1, o139 serogroups of vibrio cholerae have become a major source of pathogenic infection. however, the origin and acquisition of their virulence properties remain under explored. in this regard bacteriophages of vibrio cholerae are well known to be the carriers of pathogenic traits across various strains. so, any possible association of vibriophages and non-o1, o139 serogroups would provide a deeper insight of their pathogenic threats. | 2009 | 19789504 |
| a duplex vibriocidal assay to simultaneously measure bactericidal antibody titers against vibrio cholerae o1 inaba and ogawa serotypes. | currently available cholera vaccines are formulated with killed-whole cells of vibrio cholerae o1 inaba and ogawa serotypes. a serum vibriocidal assay has been widely used to evaluate the immunogenicity of cholera vaccines in clinical trials. in this study, we developed a duplex vibriocidal assay to obtain vibriocidal antibody titers against both serotypes simultaneously. initially, serial dilutions of serum from vaccinees were incubated with guinea pig complements along with both streptomycin-r ... | 2009 | 19796659 |
| the pathogenic properties of a novel and conserved gene product, kerv, in proteobacteria. | identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. in this study, we uncover such a factor, termed kerv, in proteobacteria. experiments carried out in a variety of eukaryotic host infection models revealed that the virulence of a pseudomonas aeruginosa kerv null mutant was compromised when it interacted with amoebae, plants, flies, and mice. bioinformatics analyses indicated that kerv is a hypothetical methyltra ... | 2009 | 19779606 |
| differential response of vibrio cholerae planktonic and biofilm cells to autoinducer 2 deficiency. | the formation of biofilm communities enhances the persistence of vibrio cholerae in aquatic environments. biofilm production is repressed by the quorum-sensing regulator hapr in response to the accumulation of cai-1 and ai-2. cai-1 is the strongest input signal activating hapr, whereas the role of ai-2 remains ill-defined. in the present study, we show that a v. cholerae luxs (ai-2-defective) mutant made increased biofilm. interestingly, cells in the biofilm were more responsive to ai-2 deficien ... | 2009 | 19780972 |
| antisecretory activity from the flowers of chiranthodendron pentadactylon and its flavonoids on intestinal fluid accumulation induced by vibrio cholerae toxin in rats. | the flowers of chiranthodendron pentadactylon larreat. (sterculiaceae) has been traditionally used as folk medicine in mexico for the treatment of gastrointestinal disorders such as diarrhea and dysentery. | 2009 | 19781621 |
| effects of enteropathogenic bacteria & lactobacilli on chemokine secretion & toll like receptor gene expression in two human colonic epithelial cell lines. | the intestinal epithelium is part of the innate immune system responding to contact with pathogenic or commensal bacteria. the objective of this study was to compare innate responses of intestinal epithelial cell lines to pathogenic bacteria and to lactobacilli. | 2009 | 19797815 |
| multiresistant vibrio cholerae non-o1/non-o139 from waters in south india: resistance patterns and virulence-associated gene profiles. | from different aquatic locations in alleppey district, kerala, south india a number (n = 36) of multiresistant non-o1, non-o139 v. cholerae strains were isolated. water samples were filtered through 0.22 mum membrane filters, enriched in alkaline peptone water and plated onto thiosulfate-citrate-bile salts-sucrose (tcbs) agar. the isolates were resistance to cefotaxime (50%), nalidixic acid (44.4%), streptomycin and tetracycline (41.6%), trimethoprim (38.8%), co-trimoxazole (33.3%), furazolidone ... | 2009 | 19810041 |
| successful small intestine colonization of adult mice by vibrio cholerae requires ketamine anesthesia and accessory toxins. | vibrio cholerae colonizes the small intestine of adult c57bl/6 mice. in this study, the physical and genetic parameters that facilitate this colonization were investigated. successful colonization was found to depend upon anesthesia with ketamine-xylazine and neutralization of stomach acid with sodium bicarbonate, but not streptomycin treatment. a variety of common mouse strains were colonized by o1, o139, and non-o1/non-o139 strains. all combinations of mutants in the genes for hemolysin, the m ... | 2009 | 19812690 |
| vibrio cholerae interactions with the gastrointestinal tract: lessons from animal studies. | vibrio cholerae is a curved gram-negative rod that causes the diarrheal disease cholera. one hundred and twenty five years of study of v. cholerae microbiology have made this lethal pathogen arguably the most well-understood non-invasive mucosal pathogen. over the past 25 years, modern molecular techniques have permitted the identification of many genes and cellular processes that are critical for v. cholerae colonization of the gastrointestinal tract. review of the literature reveals that there ... | 2009 | 19812979 |
| ai-2 does not function as a quorum sensing molecule in campylobacter jejuni during exponential growth in vitro. | campylobacter jejuni contains a homologue of the luxs gene shown to be responsible for the production of the signalling molecule autoinducer-2 (ai-2) in vibrio harveyi and vibrio cholerae. the aim of this study was to determine whether ai-2 acted as a diffusible quorum sensing signal controlling c. jejuni gene expression when it is produced at high levels during mid exponential growth phase. | 2009 | 19814796 |
| the vibrio cholerae quorum-sensing autoinducer cai-1: analysis of the biosynthetic enzyme cqsa. | vibrio cholerae, the bacterium that causes the disease cholera, controls virulence factor production and biofilm development in response to two extracellular quorum-sensing molecules, called autoinducers. the strongest autoinducer, called cai-1 (for cholera autoinducer-1), was previously identified as (s)-3-hydroxytridecan-4-one. biosynthesis of cai-1 requires the enzyme cqsa. here, we determine the cqsa reaction mechanism, identify the cqsa substrates as (s)-2-aminobutyrate and decanoyl coenzym ... | 2009 | 19838203 |
| household fish preparation hygiene and cholera transmission in monrovia, liberia. | in the 1980s vibrio cholerae was found to be an autochthonous resident of aquatic environments. as result, ingestion of undercooked, contaminated fish has been associated with cholera transmission. an alternative mechanism of transmission associated with fish was hypothesised by schürmann et al. in 2002. he described a cholera case that was more likely to have been infected by contamination on the patient's hands rather than by ingestion of contaminated fish. | 2009 | 19858575 |
| [establishment and application of a loop-mediated isothermal amplification method for rapid diagnosis of vibrio cholerae]. | to establish a loop-mediated isothermal amplification (lamp) method for rapid diagnosis of vibrio cholerae. | 2009 | 19861266 |
| [cholera epidemics in mali between 1995 and 2004]. | cholera represents a public health problem in developing countries like mali. | 2009 | 19863017 |
| vibrio cholerae o139 capsular polysaccharide confers complement resistance in the absence or presence of antibody yet presents a productive target for cell lysis: implications for detection of bactericidal antibodies. | vibrio cholerae o139 variants with different surface phenotypes have been compared for their resistance to complement and also for their susceptibility to antibody-dependent, complement-mediated bacteriolysis (acb). while both capsular polysaccharide (cps) and lipopolysaccharide (lps) contribute to complement resistance in the absence of antibody, the relative survival of variants expressing only one of these surface polysaccharides reveals cps to be of much greater significance in this respect. ... | 2009 | 19818394 |
| efficacy and safety of a modified killed-whole-cell oral cholera vaccine in india: an interim analysis of a cluster-randomised, double-blind, placebo-controlled trial. | oral cholera vaccines consisting of killed whole cells have been available for many years, but they have not been used extensively in populations with endemic disease. an inexpensive, locally produced oral killed-whole-cell vaccine has been used in high-risk areas in vietnam. to expand the use of this vaccine, it was modified to comply with who standards. we assessed the efficacy and safety of this modified vaccine in a population with endemic cholera. | 2009 | 19819004 |
| distribution and sequence analysis of virulence associated genes in vibrio cholerae o1, o139 and non-o1/non-o139 isolates from thailand. | virulence-associated genes of vibrio cholerae including o1, o139 and non-o1/non-o139 from an outbreak in songkhla province and sporadic cases occurred in thailand during 1993 - 2002 were investigated. one hundred eighty-five v. cholerae strains were examined for the presence of virulence-associated genes including ctxa, tcpa, zot, toxr, toxs, toxt, and ace by polymerase chain reaction. dna sequences of ctxa, tcpa, zot and toxr also were investigated in 8 selected isolates. results showed that th ... | 2009 | 19842384 |
| clinical outcomes in household contacts of patients with cholera in bangladesh. | multiple vibrio cholerae infections in the same household are common. the objective of this study was to examine the incidence of v. cholerae infection and associated clinical symptoms in household contacts of patients with cholera and to identify risk factors for development of severe dehydration in this cohort. | 2009 | 19842974 |
| genomic analysis of a novel integrative conjugative element in vibrio cholerae. | integrative conjugative elements (ices) are a class of self-transmissible mobile elements that mediate horizontal gene transfer in bacteria, and play an important role in bacterial evolution. since 1992, ices of the sxt/r391 family have been found to be widely distributed among vibrio cholerae strains isolated in asian countries. here we describe icevchb33, an ice found in the genomes of two v. cholerae o1 eltor strains, one isolated in india, 1994, and the other from mozambique, 2004. icevchb33 ... | 2009 | 19850044 |
| a rice-based oral cholera vaccine induces macaque-specific systemic neutralizing antibodies but does not influence pre-existing intestinal immunity. | we previously showed that oral immunization of mice with a rice-based vaccine expressing cholera toxin (ct) b subunit (mucorice-ct-b) induced ct-specific immune responses with toxin-neutralizing activity in both systemic and mucosal compartments. in this study, we examined whether the vaccine can induce ct-specific ab responses in nonhuman primates. orally administered mucorice-ct-b induced high levels of ct-neutralizing serum igg abs in the three cynomolgus macaques we immunized. although the a ... | 2009 | 19880451 |
| isolation of vibrio cholerae el tor serotype inaba in 2006 and the most common phage type of v. cholerae in mumbai. | 2009 | 19876467 | |
| identification, cloning, and functional characterization of emrd-3, a putative multidrug efflux pump of the major facilitator superfamily from vibrio cholerae o395. | a putative multidrug efflux pump, emrd-3, belonging to the major facilitator superfamily (mfs) of transporters and sharing homology with the bcr/cfla subfamily, was identified in vibrio cholerae o395. we cloned the emrd-3 gene and evaluated its role in antimicrobial efflux in a hypersensitive escherichia coli strain. the efflux activity of this membrane protein resulted in lowering the intracellular concentration of ethidium. the recombinant plasmid carrying emrd-3 conferred enhanced resistance ... | 2009 | 19876617 |
| hybrid el tor vibrio cholerae o1, kuwait. | 2009 | 19891900 | |
| structural basis of ligand binding by a c-di-gmp riboswitch. | the second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-gmp) regulates many processes in bacteria, including motility, pathogenesis and biofilm formation. c-di-gmp-binding riboswitches are important downstream targets in this signaling pathway. here we report the crystal structure, at 2.7 a resolution, of a c-di-gmp riboswitch aptamer from vibrio cholerae bound to c-di-gmp, showing that the ligand binds within a three-helix junction that involves base-pai ... | 2009 | 19898477 |
| recognition of the bacterial second messenger cyclic diguanylate by its cognate riboswitch. | the cyclic diguanylate (bis-(3'-5')-cyclic dimeric guanosine monophosphate, c-di-gmp) riboswitch is the first known example of a gene-regulatory rna that binds a second messenger. c-di-gmp is widely used by bacteria to regulate processes ranging from biofilm formation to the expression of virulence genes. the cocrystal structure of the c-di-gmp responsive gemm riboswitch upstream of the tfox gene of vibrio cholerae reveals the second messenger binding the rna at a three-helix junction. the two-f ... | 2009 | 19898478 |
| an outbreak of cholera associated with an unprotected well in parbatia, orissa, eastern india. | in november 2003, an outbreak (41 cases; attack rate-4.3%; no deaths) of severe diarrhoea was reported from a village in orissa, eastern india. thirteen of these cases were hospitalized. a matched case-control study was conducted to identify the possible exposure variables. since all wells were heavily chlorinated immediately after the outbreak, water samples were not tested. the cases were managed symptomatically. descriptive epidemiology suggested clustering of cases around one public well. vi ... | 2009 | 19902800 |
| vibrio cholerae cytolysin causes an inflammatory response in human intestinal epithelial cells that is modulated by the prtv protease. | vibrio cholerae is the causal intestinal pathogen of the diarrheal disease cholera. it secretes the protease prtv, which protects the bacterium from invertebrate predators but reduces the ability of vibrio-secreted factor(s) to induce interleukin-8 (il-8) production by human intestinal epithelial cells. the aim was to identify the secreted component(s) of v. cholerae that induces an epithelial inflammatory response and to define whether it is a substrate for prtv. | 2009 | 19907657 |
| deciphering bacterial language. | 2009 | 19915530 | |
| targeting the replication initiator of the second vibrio chromosome: towards generation of vibrionaceae-specific antimicrobial agents. | the vibrionaceae is comprised of numerous aquatic species and includes several human pathogens, such as vibrio cholerae, the cause of cholera. all organisms in this family have two chromosomes, and replication of the smaller one depends on rctb, a gene that is restricted to the vibrionaceae. given the increasing prevalence of multi-drug resistance in pathogenic vibrios, there is a need for new targets and drugs to combat these pathogens. here, we carried out a high throughput cell-based screen t ... | 2009 | 19936046 |
| g(i)-coupled gpcr signaling controls the formation and organization of human pluripotent colonies. | reprogramming adult human somatic cells to create human induced pluripotent stem (hips) cell colonies involves a dramatic morphological and organizational transition. these colonies are morphologically indistinguishable from those of pluripotent human embryonic stem (hes) cells. g protein-coupled receptors (gpcrs) are required in diverse developmental processes, but their role in pluripotent colony morphology and organization is unknown. we tested the hypothesis that g(i)-coupled gpcr signaling ... | 2009 | 19936228 |
| susceptibility to fluoroquinolones of vibrio cholerae o1 isolated from diarrheal patients in zimbabwe. | 2009 | 19952318 | |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| come together, right now. | 2009 | 19956202 | |
| simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. | we introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. this tag is comprised of the vibrio cholerae martx toxin cysteine protease domain (cpd), an autoprocessing enzyme that cleaves exclusively after a leucine residue within the target protein-cpd junction. importantly, v. cholerae cpd is specifically activated by inositol hexakisphosphate (insp(6)), a eukaryotic-specific small molecule that is absent fro ... | 2009 | 19956581 |
| activation of proinflammatory response in human intestinal epithelial cells following vibrio cholerae infection through pi3k/akt pathway. | vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. in this study, we demonstrate that v. cholerae o395 infection of intestinal epithelial cells results in the activation of akt. inhibition of akt significantly decreases il-1alpha, il-6, and tnf-alpha production in v. cholerae infected int407 cells. analysis of the mechanisms of akt influences on cytokine response demonstrates that akt promotes nf-kappab activation. we have extended these findings to show ... | 2009 | 19940940 |
| development of peru-15 (choleragarde), a live-attenuated oral cholera vaccine: 1991-2009. | epidemics of severe dehydrating cholera are on the increase in resource-limited settings around the world. adults, children and young infants are all at risk of these infections. considerable efforts have been made for the development of safe and efficacious oral cholera vaccines over the last three decades. whole-cell-inactivated as well as live oral cholera vaccines have been developed and tested in different field settings to determine the efficacy and/or effectiveness of such vaccines for re ... | 2009 | 19943759 |
| hypoxia and the hypoxic response pathway protect against pore-forming toxins in c. elegans. | pore-forming toxins (pfts) are by far the most abundant bacterial protein toxins and are important for the virulence of many important pathogens. as such, cellular responses to pfts critically modulate host-pathogen interactions. although many cellular responses to pfts have been recorded, little is understood about their relevance to pathological or defensive outcomes. to shed light on this important question, we have turned to the only genetic system for studying pft-host interactions-caenorha ... | 2009 | 20011506 |
| [non-toxigenic hemolytic vibrio cholerae non-o1 non-o139 fatal septicemia. report of one case]. | we report a 70-year-old woman, who had recently consumed shellfish, that was admitted to the intensive care unit with septic shock and died 19 hours later due to a multi-organic failure. microbiological, serological and molecular assays confirmed a hemolytic tdh+ vibrio cholerae non-01, non 0139 as the etiologic agent. | 2009 | 20011960 |
| mobile antibiotic resistance encoding elements promote their own diversity. | integrating conjugative elements (ices) are a class of bacterial mobile genetic elements that disseminate via conjugation and then integrate into the host cell genome. the sxt/r391 family of ices consists of more than 30 different elements that all share the same integration site in the host chromosome but often encode distinct properties. these elements contribute to the spread of antibiotic resistance genes in several gram-negative bacteria including vibrio cholerae, the agent of cholera. here ... | 2009 | 20019796 |
| [a duplex nested pcr assay detecting of vibrio cholerae and its application on environmental specimens]. | to establish a duplex nested pcr assay system which is capable for detecting o1 and o139 groups of vibrio cholerae simultaneously, and is applicable to environmental specimens from routine cholera surveillance. | 2009 | 20021845 |
| impact of temperature variability on cholera incidence in southeastern africa, 1971-2006. | africa has a number of climate-sensitive diseases. one that remains a threat to public health is cholera. the aquatic environment temperature is the most important ecological parameter governing the survival and growth of vibrio cholerae. indeed, recent studies indicate that global warming might create a favorable environment for v. cholerae and increase its incidence in vulnerable areas. in light of this, a poisson regression model has been used to analyze the possible association between the c ... | 2009 | 20039097 |
| molecular characterisation of vibrio cholerae o1 strains carrying an sxt/r391-like element from cholera outbreaks in kenya: 1994-2007. | over the last decade, cholera outbreaks in parts of kenya have become common. although a number of recent studies describe the epidemiology of cholera in kenya, there is paucity of information concerning the diversity and occurrence of mobile genetic elements in vibrio cholerae strains implicated in these outbreaks. a total of 65 vibrio cholerae o1 el tor serotype inaba isolated between 1994 and 2007 from various outbreaks in kenya were investigated for mobile genetic elements including integron ... | 2009 | 20040104 |
| comparative ice genomics: insights into the evolution of the sxt/r391 family of ices. | integrating and conjugative elements (ices) are one of the three principal types of self-transmissible mobile genetic elements in bacteria. ices, like plasmids, transfer via conjugation; but unlike plasmids and similar to many phages, these elements integrate into and replicate along with the host chromosome. members of the sxt/r391 family of ices have been isolated from several species of gram-negative bacteria, including vibrio cholerae, the cause of cholera, where they have been important vec ... | 2009 | 20041216 |
| enteric pathogens in hiv/aids from a tertiary care hospital. | patterns of enteric infections in hiv in developing countries may differ in several important ways from developed countries, the knowledge of which can often guide therapy when resource limitations hamper the exact diagnosis of the etiological agent in hiv-associated diarrhea. | 2009 | 20049303 |
| familial aggregation of vibrio cholerae-associated infection in matlab, bangladesh. | vibrio cholerae is a major cause of diarrhoeal illness in endemic regions, such as bangladesh. understanding the factors that determine an individual's susceptibility to infection due to v. cholerae may lead to improved prevention and control strategies. increasing evidence suggests that human genetic factors affect the severity of v. cholerae-associated infection. this study, therefore, sought to characterize the heritable component of susceptibility to infection due to v. cholerae using the ma ... | 2009 | 20099756 |
| assessment of the evolutionary origin and possibility of crispr-cas (cass) mediated rna interference pathway in vibrio cholerae o395. | bacteria have developed several defense mechanisms against bacteriophages over evolutionary time, but the concept of prokaryotic rna interference mediated defense mechanism against phages and other invading genetic elements has emerged only recently. clustered regularly interspaced short palindromic repeats (crispr) together with closely associated genes (cas genes) constitute the cass system that is believed to provide a rnai-like defense mechanism against bacteriophages within the host bacteri ... | 2009 | 20109154 |
| [a multipathogen selective enrichment broth (svv) for simultaneous growth of salmonella, vibrio parahaemolyticus, and vibrio cholerae]. | we formulated a selective enrichment broth (svv) for simultaneous growth of salmonella, vibrio parahaemolyticus, and vibrio cholerae by single factor experiment and response surface method. we evaluated the enrichment effect of svv by conventional culture method and real-time pcr assay. we obtained the svv broth by supplementting the buffered peptone water (bpw) with bile salt no. 3, potassium tellurite, and sodium citrate as inhibitors, and glucose, mannitol, snhydrous sodium sulfite and sodium ... | 2009 | 20112694 |
| molecular characterization of serogrouping and virulence genes of malaysian vibrio cholerae isolated from different sources. | a pair of primers targeting the hlya gene for vibrio cholerae which could distinguish the classical from el tor biotypes was designed and combined with other specific primers for ompw, rfb complex, and virulence genes such as ctxa, toxr, and tcpi in a multiplex pcr (m-pcr) assay. this m-pcr correctly identified 39 v. cholerae from clinical, water and seafood samples. the efficiency of this multiplex pcr (m-pcr) was compared with conventional biochemical and serogrouping methods. one o139 and 25 ... | 2009 | 20118606 |
| the francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. | francisella tularensis causes the human disease tularemia. f. tularensis is able to survive and replicate within macrophages, a trait that has been correlated with its high virulence, but it is unclear the exact mechanism(s) this organism uses to escape killing within this hostile environment. f. tularensis virulence is dependent upon the francisella pathogenicity island (fpi), a cluster of genes that we show here shares homology with type vi secretion gene clusters in vibrio cholerae and pseudo ... | 2009 | 20054881 |
| [laboratory diagnosis of cholera: analysis and prospects for improvement]. | microbiological monitoring of the circulation of vibro cholerae remains one of the key factors contributing to optimization of epidemiological surveillance in a specific area and the laboratory diagnosis of cholera is a basic applied tool for the detection and characterization of isolated cultures. the quality of etiological identification of the pathogen, the competent use of procedures, and the observance of a laboratory diagnosis scheme increases the likelihood of the cholera pathogen being d ... | 2009 | 20030273 |
| repeated dose toxicity study of a live attenuated oral cholera vaccine in sprague dawley rats. | a live attenuated vaccine candidate against human cholera has been developed from the genetically modified vibrio cholerae o1, biotype el tor, serotype ogawa, 638 strain. previous single dose toxicity and local tolerance studies have demonstrated that the product is innocuous in sprague dawley rats by oral route and single dose. the present paper describes a repeated dose toxicity study using a further dose compared to the proposed clinical schedule. | 2009 | 20082865 |
| [isolation and characterization of shewanella spp. from patients of food poisoning]. | to identify the isolates of shewanella spp. from specimens of food poisoning based on biological and biochemical analysis. | 2009 | 20193210 |
| [secretion expression of cholera toxin b subunit in food-grading lactococcus lactis expression system.]. | to clone and secretion express cholera toxin b subunit (ctb) in food-grading lactococcus lactis expression systems. | 2009 | 20193317 |
| preparation of sio2/polymethyl methacrylate/fe3o4 nanoparticles and its application in detecting e. coli o157:h7 using chemiluminescent immunological method. | this paper described that fe3o4 nanoparticles were encapsulated with methyl methacrylate (mma) using linolenic acid (la) as a crosslinking agent, after which the resulting polymethyl methacrylate (pmma) embed fe3o4 nanoparticles (pmma/fe3o4) were coated with silica, forming sio2/ (pmma/fe3o4) core-shell structure particles. then these magnetic nanoparticles (mnps) were applied in the developed system of chemiluminescent magnetic enzyme-linked immunoassay. e. coli o157:h7 was sandwiched between r ... | 2009 | 20201424 |
| vibriocidal activity of certain medicinal plants used in indian folklore medicine by tribals of mahakoshal region of central india. | screening of the medicinal plants and determination of minimum inhibitory concentration (mic) against vibrio cholerae and vibrio parahaemolyticus. | 2009 | 20442821 |