Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
impacts of pulsed electric field and heat treatment on quality and sensory properties and microbial inactivation of pomegranate juice.synergistic effects of pulsed electric field+mild heat on quality properties of pomegranate juice were modeled using the best-fit multiple (non-) linear regression models with inactivation kinetics parameters of escherichia coli o157:h7 and staphylococcus aureus. no significant difference was detected between the control and the treated samples in terms of ph; °brix; total antioxidant capacity; total monomeric anthocyanin content; total ascorbic acid concentration; and the sensory properties of ...201728610446
sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry.a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ...201728590745
multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts.multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.201728743314
bactericidal effect of 266 to 279nm wavelength uvc-leds for inactivation of gram positive and gram negative foodborne pathogenic bacteria and yeasts.recently, uvc-led technology has been validated as an alternative to irradiation with conventional mercury uv lamps. in this study, we sought to determine primary factors affecting reduction trends shown in several microorganisms. four major foodborne pathogens (escherichia coli o157:h7, salmonella spp. listeria monocytogenes, staphylococcus aureus) and spoilage yeasts (saccharomyces pastorianus, pichia membranaefaciens), important to the brewing industry, were inoculated onto selective and non- ...201728578053
instant green synthesis of silver-based herbo-metallic colloidal nanosuspension in terminalia bellirica fruit aqueous extract for catalytic and antibacterial applications.in the present study, microwave-assisted, optimized, instant, terminalia bellirica fruit extract-mediated green synthesis of colloidal silver nanoparticles (agnps) has been reported. the synthesized agnps were characterized by uv-vis spectroscopy, ftir, zetasizer, fesem, edx and xrd. the characteristic surface plasmon peak of reaction mixture at 406 nm confirmed the synthesis of agnps. the ftir studies confirmed phytoconstituents were responsible for the synthesis and stability of agnps. the fes ...201728409425
production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays.in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ...201728536016
[synthesis of antibiotic loaded polylactic acid nanoparticles and their antibacterial activity against escherichia coli o157:h7 and methicillin-resistant staphylococcus aureus].polymeric nanoparticles are promising nanotechnology tools to fight pathogenic bacteria resistant to conventional antibiotics.201728527243
efficacy of (+)-lariciresinol to control bacterial growth of staphylococcus aureus and escherichia coli o157:h7.this study was undertaken to assess the antibacterial potential of a polyphenolic compound (+)-lariciresinol isolated from rubia philippinensis against selected foodborne pathogens staphylococcus aureus kctc1621 and escherichia coli o157:h7. (+)-lariciresinol at the tested concentrations (250 μg/disk) evoked a significant antibacterial effect as a diameter of inhibition zones (12.1-14.9 mm) with minimum inhibitory concentration (mic), and minimum bactericidal concentration values of 125-250 and ...201728515721
reduction of reactive red 241 by oxygen insensitive azoreductase purified from a novel strain staphylococcus ku898286.an oxygen insensitive azoreductase was purified from a novel bacterial strain (staphylococcus sp. ku898286) that was isolated from an abandoned site of the textile waste discharge unit. the isolated enzyme had efficiently cleaved the azo-bonds through reductive transformation under aerobic conditions. initial phenotypic characterization and final construction of phylogenetic tree on the basis of 16s rdna demonstrated 99% resemblance of the isolate to staphylococcus aureus. the purified azoreduct ...201728467413
neo-actinomycins a and b, natural actinomycins bearing the 5h-oxazolo[4,5-b]phenoxazine chromophore, from the marine-derived streptomyces sp. imb094.neo-actinomycins a and b (1 and 2), two new natural actinomycins featuring an unprecedented tetracyclic 5h-oxazolo[4,5-b]phenoxazine chromophore, were isolated from the marine-derived actinomycete streptomyces sp. imb094. their structures were elucidated by spectroscopic analyses. the presence of this ring system was proposed to originate from a condensation between actinomycin d (3) with α-ketoglutarate and pyruvate, respectively. compound 1 showed potent cytotoxic activities against human canc ...201728620204
endogenous bacterial endophthalmitis. a report of 2cases.the cases are presented on 2patients with bacterial endogenous endophthalmitis. the first one was caused by streptococcus bovis, developed after colonoscopy, which had a poor outcome and resulted in evisceration. the second case was caused by a methicillin resistant staphylococcus aureus from an arthrodesis complicated with a para-spinal abscess. it had an excellent visual outcome.201728427732
[brain abscess in children: ten years of experience in a third level pediatric hospital].brain abscess is a focal infection that occurs with a frequency of 0.3-1.3 cases per 100,000 people/year. we describe the epidemiology, clinical and microbiology characteristics of 38 children diagnosed with brain abscess hospitalized between 4/1/2005 and 12/31/2015 at hospital de pediatría "prof. dr. juan p. garrahan" in buenos aires city. twenty-four patients were male. the median age was 132 months. predisposing factors were detected in 25 children. the median evolution from onset of symptoms ...201728737874
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria.emerging resistance to antibiotics is a mounting worldwide health concern and increases the need for nonantibiotic strategies to combat infectious diseases caused by bacterial pathogens. in this study, the authors used the antibiofilm activity of the naturally occurring antimicrobial cinnamaldehyde (cnma) conjugated to the surface of gold nanoparticles (gnps) to deliver cnma efficiently and eradicate biofilms of gram-negative organisms (enterohemorrhagic escherichia coli o157:h7, and pseudomonas ...201728435260
update on the epidemiology and antibiotic resistance of ocular infections.the purpose of this review is to provide an update on the epidemiology and current antibiotic-resistant threats in ophthalmology.201728546690
the changing epidemiology and clinical features of infective endocarditis: a retrospective study of 196 episodes in a teaching hospital in china.infective endocarditis is an uncommon but life-threatening infectious disease. to our knowledge, current investigations of the characteristics of infective endocarditis in our region are scarce. in this study, we aimed to investigate the changes in the epidemiology and clinical features of infective endocarditis.201728482820
antibacterial action of jineol isolated from scolopendra subspinipes mutilans against selected foodborne pathogens.this study was undertaken to assess the antibacterial potential of 3,8-dihydroxyquinoline (jineol) isolated from scolopendra subspinipes mutilans against selected foodborne pathogens escherichia coli o157:h7 and staphylococcus aureus kctc-1621. jineol at the tested concentration (50 μl; corresponding to 250 μg/disk) exhibited significant antibacterial effects as a diameter of inhibition zones (11.6-13.6 mm), along with minimum inhibitory concentration (mic) and minimum bactericidal concentration ...201728400770
progress in definition, prevention and treatment of fungal infections in cystic fibrosis.cystic fibrosis (cf) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. the main bacterial species causing infections include pseudomonas aeruginosa, staphylococcus aureus, haemophilus influenzae and achromobacter xy ...201728762125
air pollution exposure is associated with mrsa acquisition in young u.s. children with cystic fibrosis.the role of air pollution in increasing susceptibility to respiratory tract infections in the cystic fibrosis (cf) population has not been well described. we recently demonstrated that chronic pm2.5 exposure is associated with an increased risk of initial pseudomonas aeruginosa acquisition in young children with cf. the purpose of this study was to determine whether pm2.5 exposure is a risk factor for acquisition of other respiratory pathogens in young children with cf.201728750627
seasonality of acquisition of respiratory bacterial pathogens in young children with cystic fibrosis.seasonal variations are often observed for respiratory tract infections; however, limited information is available regarding seasonal patterns of acquisition of common cystic fibrosis (cf)-related respiratory pathogens. we previously reported differential seasonal acquisition of pseudomonas aeruginosa in young children with cf and no such variation for methicillin-susceptible staphylococcus aureus acquisition. the purpose of this study was to describe and compare the seasonal incidence of acquis ...201728599639
comparison of a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform, asta microidsys, with bruker biotyper for species identification.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, with its accuracy and speed, is widely used for bacterial identification. the asta microidsys system (asta, korea) was recently developed for species identification. we compared its performance with that of bruker biotyper (bruker daltonics, germany). microbes were recovered from sputum, urine, and pus samples from patients admitted to a tertiary care hospital in korea from january to april 2016. matrix solution (α-cya ...201728840993
application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products.foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ...201728825446
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces.air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ...201728803189
bacterial and histopathological findings in deep head and neck infections: a retrospective analysis.deep neck infections are among the most dangerous acute diseases in the head and neck region. this analysis gives an overview of the bacterial and histopathologic findings of deep neck infections.201728411005
next-generation sequencing to monitor the spread of antimicrobial resistance.next-generation sequencing is increasingly being used to monitor current and historic events related to the emergence and spread of antimicrobial resistance. in a recent publication, researchers analyzed the rise of methicillin-resistant staphylococcus aureus in the 1960s, emphasizing that adaptations conferring antibiotic resistance might pre-date the introduction of novel antibiotic derivatives. other researchers have evaluated the role of transmission within a healthcare network, using the ex ...201728738906
textural, structural and biological evaluation of hydroxyapatite doped with zinc at low concentrations.the present work was focused on the synthesis and characterization of hydroxyapatite doped with low concentrations of zinc (zn:hap) (0.01 < xzn < 0.05). the incorporation of low concentrations of zn(2+) ions in the hydroxyapatite (hap) structure was achieved by co-precipitation method. the physico-chemical properties of the samples were characterized by x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), x-ray photoelectron spectroscopy (xps), scanning electron microscopy (s ...201728772589
development of poly(lactic acid)/chitosan fibers loaded with essential oil for antimicrobial applications.cinnamon essential oil (ceo) was successfully encapsulated into chitosan (cs) nanoparticles at different loading amounts (1%, 1.5%, 2%, and 2.5% v/v) using oil-in-water (o/w) emulsion and ionic-gelation methods. in order to form active packaging, poly(lactic acid) (pla) was used to fabricate pla/cs-ceo composite fibers using a simple electrospinning method. the shape, size, zeta potential, and encapsulation efficacy of the cs-ceo nanoparticles were investigated. the composition, morphology, and ...201728737719
comparative analysis of the composition and active property evaluation of certain essential oils to assess their potential applications in active food packaging.the antifungal, antibacterial, and antioxidant activity of four commercial essential oils (eos) (thyme, clove, rosemary, and tea tree) from romanian production were studied in order to assess them as bioactive compounds for active food packaging applications. the chemical composition of the oils was determined with the folin-ciocâlteu method and gas chromatography coupled with mass spectrometry and flame ionization detectors, and it was found that they respect the afnor/iso standard limits. the ...201728772407
antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: a novel and potential wound dressing.wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. in this study, the advantages of these materials were combined and alginate films were crosslinked with cerium(iii) solution and chitosan added cerium(iii) solution. films were characterized by fourier transform infrared spectroscopy (ftir), light tra ...201728751050
[pyogenic liver abscess: changes of organisms and consequences for diagnosis and therapy].liver abscess is a rare but potentially fatal disease with mortality rates of 2 - 12 %. the spectrum of causative microorganisms varies across the world and is changing due to increasing worldwide travel activity. as a result, previously less frequent pathogens must increasingly be considered in the differential diagnosis. the most common pathogens of liver abscess are so-called abscess streptococci, anaerobes and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae. certain ...201728728202
synthesis and in vitro evaluation of peracetyl and deacetyl glycosides of eugenol, isoeugenol and dihydroeugenol acting against food-contaminating bacteria.essential oils, as well as their separate components, have shown promise as alternatives to synthetic preservatives. therefore, it would be interesting to optimize the effect of these compounds and to evaluate their applicability as additives in food. to this end, six peracetyl and deacetyl glycosides were synthesized from eugenol, isoeugenol and dihydroeugenol. all of the glycosides were characterized by ir and nmr. the synthesized compounds and their aglycones were evaluated to determine their ...201728763946
inactivation of microbicidal active halogen compounds by sodium thiosulphate and histidine/methionine for time-kill assays.rapid inactivation of antimicrobial test agents after exact incubation times with microorganisms is required in time-kill assays. sodium thiosulphate and a combination of methionine and histidine were compared for neutralisation of active halogen compounds. test oxidants were mixed with surplus sodium thiosulphate (3%-6%) or histidine/methionine (1% each) in phosphate-buffered saline and incubated for different times, followed by addition of staphylococcus aureus, escherichia coli, or pseudomona ...201728760586
staphylococcus aureus single-stranded dna-binding protein ssba can bind but cannot stimulate pria helicase.single-stranded dna-binding protein (ssb) and pria helicase play important roles in bacterial dna replication restart process. the mechanism by which pria helicase is bound and stimulated by ssb in escherichia coli (ec) has been established, but information on this process in gram-positive bacteria are limited. we characterized the properties of ssb from staphylococcus aureus (sassba, a counterpart of ecssb) and analyzed its interaction with sapria. the gel filtration chromatography analysis of ...201728750050
antibacterial electrospun chitosan-based nanofibers: a bacterial membrane perforator.this study investigates the antibacterial action of chitosan-based nanofibers (cnfs) obtained by the electrospinning process on the permeability of bacterial membranes. the bactericidal efficiency of cnfs was first determined against gram-negative escherichia coli and salmonella typhimurium, and gram-positive staphylococcus aureus and listeria innocua bacteria as a baseline. the results strongly suggest that cnfs interact with the negatively charged bacterial cell wall causing membrane rupture a ...201728748074
spectroscopic characterization and nanosafety of ag-modified antibacterial leather and leatherette.the development of antibacterial coatings is of great interest from both industry and the consumer's point of view. in this study, we characterized tanned leather and polyurethane leatherette, typically employed in the automotive and footwear industries, which were modified by photo-deposition of antibacterial silver nanoparticles (agnps). material surface chemical composition was investigated in detail by x-ray photoelectron spectroscopy (xps). the material's antibacterial capability was checke ...201728758912
biomimetic synthesis of agnps from penicillium chrysogenum strain fgcc/bls1 by optimising physico-cultural conditions and assessment of their antimicrobial potential.biomimetic synthesis of metal nanoparticles (nps) is safe and eco-friendly; therefore, find diverse applications. considering this, the soil fungi penicillium chrysogenum strain fungal germplasm collection centre/ bls1 was isolated, characterized and explored to synthesize extracellular silver nps (agnps) under optimised conditions. the synthesis of agnps was investigated using ultraviolet (uv)-visible spectroscopy, fourier-transform infra-red spectroscopy (ftir), transmission electron microscop ...201728745292
green synthesis of gold nanoparticles using citrus maxima peel extract and their catalytic/antibacterial activities.the peel of citrus maxima (c. maxima) is the primary byproducts during the process of fruit or juice in food industries, and it was always considered as biomass waste for further treatments. in this study, the authors reported a simple and eco-friendly method to synthesise gold nanoparticles (aunps) using c. maxima peel extract as reducing and capping agents. the synthesised aunps were characterised by uv-visible spectrum, x-ray diffraction (xrd), transmission electron microscope (tem) and fouri ...201728745284
superhydrophobic and white light-activated bactericidal surface through a simple coating.bacterial adhesion and proliferation on surfaces are a challenge in medical and industrial fields. here, a simple one-step technique is reported to fabricate self-cleaning and bactericidal surfaces. white, blue, and violet paints were produced using titanium dioxide nanoparticles, 1h,1h,2h,2h-perfluorooctyltriethoxysilane, crystal violet, toluidine blue o, and ethanol solution. all of the painted surfaces showed superhydrophobicity in air, and even after hexadecane oil contamination, they retain ...201728758725
determinants of implementation of isolation precautions against infections by multidrug-resistant microorganisms: a hospital-based, multicenter, observational study.objectives we aimed to ascertain the factors associated with lack of isolation precautions (ip) in patients infected or colonized by third-generation cephalosporin-resistant enterobacteriaceae (3gcr-e) and methicillin-resistant staphylococcus aureus (mrsa) in hospital settings. design prospective surveillance and audit of practices. setting the study included 4 university hospitals in lyon, france. participants all patients hospitalized between april and june in 2013 and 2015 were included. case ...201728758615
anti-adhesion and anti-biofilm potential of organosilane nanoparticles against foodborne pathogens.nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. to this direction, biofilm biological cycle of salmonella typhimurium, listeria monocytogenes, escherichia coli o157:h7, staphylococcus aureus, and yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. to achieve this, four different comm ...201728744277
the analysis of etiology and risk factors for 192 cases of neonatal sepsis.this study aimed to investigate the etiology and risk factors of neonatal sepsis. a retrospective analysis was conducted on 192 patients with sepsis from august 2013 to march 2015. one hundred and six healthy neonates were used as the control group. logistic regression was used to analyze the risk factors and roc curve analysis performed in laboratory which indicated a significant correlation. the results of univariate analysis showed that postnatal age, body weight, and parity were significantl ...201728758124
public health and healthcare-associated risk of electric, warm water bidet toilets.in recent years, installation of bidet toilets within hospitals in japan has raised concerns regarding potential for cross-contamination by antimicrobial resistant bacteria from patients who are hospitalized over an extended period.201728756169
formation of matricaria chamomilla extract-incorporated ag nanoparticles and size-dependent enhanced antimicrobial property.various concentrations of matricaria chamomilla (m. chamomilla) flower extract was used for biosynthesis of ag nps with different sizes, 70±5 (ag np-1), 52±5 (ag np-1) and 37±4nm (ag np-3), and size-specific antimicrobial activity of them was evaluated towards gram+ staphylococcus aureus (s. aureus), gram- escherichia coli (e. coli) bacteria and candida fungus albicans (c. albicans). we also examine the formation of ag nps as a function of the plant extract volume, ag ion concentration (ag(+)) a ...201728756155
rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray.there is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. discovery of novel anti-adhesive molecules from natural product libraries will require the use of a high throughput screening platform. avian egg white (ew) provides nutrition for the embryo and protects against infection, with glycosylation responsible for binding certain pathogens. in this study, a microarray platform of 78 species of a ...201728743896
antimicrobial effects of silver nanoparticles synthesized by fatsia japonica leaf extracts for preservation of citrus fruits.due to their potent antimicrobial activity, silver nanoparticles (agnps) synthesized by biomass might be promising in food preservation, whereas effects of agnps on penicillium italicum-induced rot of citrus fruits have not been investigated. in this study, a novel agnps biosynthesis method was developed based on fatsia japonica leaf extracts. it was revealed that concentrations of leaf extracts, agno3 and nacl affected agnps yields and particle sizes obviously. under the optimized conditions (8 ...201728727146
antimicrobial properties of selected copper alloys on staphylococcus aureus and escherichia coli in different simulations of environmental conditions: with vs. without organic contamination.background: hospital equipment made from copper alloys can play an important role in complementing traditional methods of disinfection. aims of the study: the aim of this study was to assess the dynamics of the antimicrobial properties of selected copper alloys in different simulations of environmental conditions (with organic contamination vs. without organic contamination), and to test alternatives to the currently used testing methods. materials and methods: a modification of japanese standar ...201728726753
isolation and characterization of antimicrobial compound from the stem-bark of the traditionally used medicinal plant adenium obesum.medicinal plants constitute a natural reservoir for medicines worldwide. they serve mainstream therapeutics and are central in folklore medicine. in case of adenium obesum (lav, apocynaceae), indigenous people of oman use it for the treatment of venereal diseases, wounds, skin diseases, headaches, muscle pain as well as joint pain; yet, the active ingredients have not been classified. to screened the antioxidant and antimicrobial activities of an identified compound that we isolated from the hig ...201728725623
wheat germ agglutinin and lens culinaris agglutinin sensitized anisotropic silver nanoparticles in detection of bacteria: a simple photometric assay.efficacy of anisotropic silver nanoparticles sensitized with wheat germ agglutinin (wga) and lens culinaris agglutinin (lca) was studied for detection of staphylococcus aureus and escherichia coli. it was demonstrated that interaction of these nanoparticles with bacteria stabilizes them and prevents their aggregation upon addition of sodium chloride; such stabilization depends on bacteria concentration. high concentration of bacteria results in higher stabilization whereas low concentration lead ...201728693732
chemical composition and cytotoxic and antibacterial activities of the essential oil of aloysia citriodora palau grown in morocco.the aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of aloysia citriodora palau, harvested in different regions of morocco. the chemical profile was established using gas chromatography-mass spectrometry analysis. the cytotoxic activity against p815, mcf7, and vero cell lines as well as the normal human peripheral blood mononuclear cells (pbmcs) was evaluated using the mtt assay. standard, atcc, strains of bacteria (escherichia coli, stap ...201728694822
reinforcement of an antimicrobial stewardship task force aims at a better use of antibiotics of last resort: the colitifos study.physicians are facing a worldwide increase in multidrug-resistant (mdr) organisms. eradication of such bacteria, including so called superbugs (xdr), may cause physicians to prescribe last-resort antibiotics. however, experience with these drugs is limited and few data are available.201728694232
effect of experimentally increased protein supply to postpartum dairy cows on plasma protein synthesis, rumen tissue proliferation, and immune homeostasis.the effect of experimentally increasing the postpartum protein supply on plasma protein synthesis, rumen tissue proliferation, and immune homeostasis was studied using 8 periparturient holstein cows in a complete randomized design. at calving, cows were assigned to abomasal infusion of water (ctrl) or casein (cas) in addition to a lactation diet. casein infusion was gradually decreased from 696 ± 1 g/d at +2 d relative to calving (drtc) to 212 ± 10 g/d at +29 drtc to avoid excessive supply. synt ...201728727010
selective biosensing of staphylococcus aureus using chitosan quantum dots.selective biosensing of staphylococcus aureus (s. aureus) using chitosan modified quantum dots (cts@cds qds) in the presence of hydrogen peroxide is reported. the method is based on the intrinsic positive catalase activity of s. aureus. cts@cds quantum dots provide high dispersion in aqueous media with high fluorescence emission. staphylococcus aureus causes a selective quenching of the fluorescence emission of cts@cds qds in the presence of h2o2 compared to other pathogens such as escherichia c ...201728689078
molecular docking, pass analysis, bioactivity score prediction, synthesis, characterization and biological activity evaluation of a functionalized 2-butanone thiosemicarbazone ligand and its complexes.2-butanone thiosemicarbazone ligand was prepared by condensation reaction between thiosemicarbazide and butanone. the ligand was characterized by (1)h nmr, (13)c nmr, ft-ir, mass spectrometry and uv spectroscopic studies. docking studies were performed to study inhibitory action against topoisomerase ii (topo ii) and ribonucleoside diphosphate reductase (rr) enzymes. inhibition constants (ki ) of the ligand were 437.87 and 327.4 μm for the two enzymes, respectively. the ligand was tested for its ...201728684996
accurate detection of methicillin-resistant staphylococcus aureus in mixtures utilizing single bacterial duplex droplet digital pcr.accurate and rapid identification of methicillin-resistant staphylococcus aureus (mrsa) is needed to screen mrsa carriers and improve treatment. the current widely used duplex pcr methods are not able to differentiate mrsa from the co-existence of methicillin-susceptible s. aureus (mssa) and other methicillin-resistant staphylococci in this study, we aimed to develop a direct method for accurate and rapid detection of mrsa in clinical samples from open environments, such as nasal swab. the new m ...201728724560
antibiotic resistance in young children in kilosa district, tanzania 4 years after mass distribution of azithromycin for trachoma control.mass administration of azithromycin (mda) is integral to trachoma control. recent studies suggest that mda may increase drug-resistant pathogens, yet findings from prior studies suggest little long-term impact on resistance. this disparity may be linked to differences in pre-mda community-level resistance patterns. we describe carriage prevalence and antibiotic resistance patterns for streptococcus pneumoniae (spn) (nasopharyngeal swab collection), staphylococcus aureus (sa) (nasopharyngeal swab ...201728722638
multidrug-resistant microorganisms colonizing lower extremity wounds in patients in a tertiary care hospital, lima, peru.multidrug-resistant organism (mdro) infections cause high morbidity and mortality, and high costs to patients and hospitals. the study aims were to determine the frequency of mdro colonization and associated factors in patients with lower-extremity wounds with colonization. a cross-sectional study was designed during november 2015 to july 2016 in a tertiary care hospital in lima, peru. a wound swab was obtained for culture and susceptibility testing. mdro colonization was defined if the culture ...201728722595
in vitro evaluation of chitosan-coated liposome containing both coenzyme q10 and alpha-lipoic acid: cytotoxicity, antioxidant activity, and antimicrobial activity.chitosan-coated liposome containing both coenzyme q10 and alpha-lipoic acid (ccal) was a novel multifunctional nano delivery system exhibited long-term stability at room temperature and high encapsulation efficiency. which containing two kinds of antioxidants (coenzyme q10 and alpha-lipoic acid) and two antibacterial agents (chitosan and alpha-lipoic acid). however, the further biocompatibility, such as cytotoxicity and antioxidant activity, and antimicrobial activity of ccal, has not been syste ...201728722258
isolation and in vitro evaluation of bacteriophages against mdr-bacterial isolates from septic wound infections.multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. the use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. pus samples were aseptically collected from rajiv gandhi institute of medical ...201728719657
bacteriology of diabetic foot in tertiary care hospital; frequency, antibiotic susceptibility and risk factors.diabetic foot being one of the frequent and disabling complications of diabetes. in view of widespread regional variation in causative organisms and antimicrobial susceptibility, the current study aimed to determine frequency of causative organisms, their antimicrobial susceptibility and associated risk factors.201728718238
bacterial infection imaging with [(18)f]fluoropropyl-trimethoprim.there is often overlap in the diagnostic features of common pathologic processes such as infection, sterile inflammation, and cancer both clinically and using conventional imaging techniques. here, we report the development of a positron emission tomography probe for live bacterial infection based on the small-molecule antibiotic trimethoprim (tmp). [(18)f]fluoropropyl-trimethoprim, or [(18)f]fptmp, shows a greater than 100-fold increased uptake in vitro in live bacteria (staphylococcus aureus, ...201728716936
a transparent wound dressing based on bacterial cellulose whisker and poly(2-hydroxyethyl methacrylate).the current study was aimed to develop a transparent wound dressing comprised of bacterial cellulose (bc) and poly (2-hydroxyethyl methacrylate) (phema) hydrogel coated with silver (ag) nanoparticles. briefly, different concentrations of bc whiskers (bcws) were added into the hema solution to form phema/bcws hydrogel with volume ratio of monomer hema and bcws as 7:3 and 1:1. the addition of bcws into phema matrix improved its equilibrium water content and light transmittance about 20%-40% and 10 ...201728716748
synergistic bacteria killing through photodynamic and physical actions of graphene oxide/ag/collagen coating.researchers have widely agreed that the broad spectrum antibacterial activity of silver nanoparticles (agnps) can be predominantly ascribed to the action of ag(+). this study marks the first report detailing the rapid and highly efficient synergistic bacteria killing of agnps, which is achieved by inspiring agnps' strong photocatalytic capability to rapidly produce radical oxygen species using 660 nm visible light together with the innate antimicrobial ability of ag(+). these agnps were uniforml ...201728715631
a novel chimeric endolysin with antibacterial activity against methicillin-resistant staphylococcus aureus.cysteine/histidine-dependent amidohydrolase/peptidase (chap) and amidase are known as catalytic domains of the bacteriophage-derived endolysin lysk and were previously reported to show lytic activity against methicillin-resistant staphylococcus aureus (mrsa). in the current study, the in silico design and analysis of chimeric chap-amidase model was applied to enhance the stability and solubility of protein, which was achieved through improving the properties of primary, secondary and tertiary st ...201728713777
a novel antibacterial peptide derived from crocodylus siamensis hemoglobin hydrolysate induces membrane permeabilisation causing iron dysregulation, oxidative stress and bacterial death.a novel antibacterial peptide from crocodylus siamensis hemoglobin hydrolysate (chhs) was characterised for antimicrobial activity.201728712131
evidence for the presence of cell-surface-bound and intracellular bactericidal toxins in the dinoflagellate heterocapsa circularisquama.heterocapsa circularisquama, a harmful dinoflagellate, has multiple haemolytic toxins that are considered to be involved in the toxic mechanism against shellfish and certain species of zooplankton. to evaluate the further nature of the toxins of h. circularisquama, we investigated its effects on several species of bacteria. by colony formation assay, we found that h. circularisquama had antibacterial activity toward the marine bacterium vibrio alginolyticus in a cell density-dependent manner. wh ...201728654772
short communication: effects of an immunomodulatory feed additive on phagocytic capacity of neutrophils and relative gene expression in circulating white blood cells of transition holstein cows.high-producing dairy cows typically experience immunosuppression with dysregulated neutrophil function (e.g., compromised phagocytosis) during the transition period (3 wk before to 3 wk after parturition), which is causally associated with increased risk of infections. enhanced neutrophil immune competence has significant bearing with the wellbeing of transition dairy cattle. in the current study, we investigated the effect of omnigen-af (og; phibro animal health, quincy, il) and its dose in mod ...201728711260
efficient bacterial inactivation with z-scheme agi/bi2moo6 under visible light irradiation.a novel z-scheme agi/bi2moo6 hybrid photocatalyst was fabricated via a solvothermal-precipitation approach to disinfect bacteria in water. powder x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps), scanning electron microscopic (sem) equipped with energy-dispersive x-ray spectroscopy (edx), high resolution transmission electron microscope (hrtem), uv-vis diffuse reflectance spectra (drs), as well as photoluminescence spectra (pl) were employed to characterize the fabricated photocat ...201728709107
povidone-iodine-based polymeric nanoparticles for antibacterial applications.as microbial contamination is becoming more and more serious, antibacterial agents play an important role in preventing and removing bacterial pathogens from microbial pollution in our daily life. to solve the issues with water solubility and antibacterial stability of pvp-i2 (povidone-iodine) as a strong antibacterial agent, we successfully obtain hydrophobic povidone-iodine nanoparticles (povidone-iodine nps) by a two-step method related to the advantage of nanotechnology. first, the synthesis ...201728707872
correlation of edge truncation with antibacterial activity of plate-like anisotropic silver nanoparticles.the effect of silver nanoparticle anisotropy on the antibacterial properties has been studied against escherichia coli, staphylococcus aureus, bacillus, vibrio cholerae, and streptococcus pyogenes. anisotropic silver nanoparticles have been synthesized by solvothermal process. the uv-visible absorption, x-ray diffraction, and tem studies show the anisotropic nature of silver nanoparticles. the results demonstrate that the anisotropic silver nanoparticles undergo a shape-dependent interaction wit ...201728707245
silver nanoparticles functionalized with ampicillin.the resistance of pathogenic bacteria to antibiotics has become a serious problem. the emphasis is placed on the development of new, effective antimicrobial strategies. one of them is the use of agnps in association with antibiotic drugs. the aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. to confirm the occurrence of silver nanopartic ...201728704596
disinfection of the water borne pathogens escherichia coli and staphylococcus aureus by solar photocatalysis using sonochemically synthesized reusable ag@zno core-shell nanoparticles.water borne pathogens present a threat to human health and their disinfection from water poses a challenge, prompting the search for newer methods and newer materials. disinfection of the gram-negative bacterium escherichia coli and the gram-positive coccal bacterium staphylococcus aureus in an aqueous matrix was achieved within 60 and 90 min, respectively, at 35 °c using solar-photocatalysis mediated by sonochemically synthesized ag@zno core-shell nanoparticles. the efficiency of the process in ...201728698514
concordance of results of blood and tissue cultures from patients with pyogenic spondylitis: a retrospective cohort study.to investigate the concordance of results of blood and tissue cultures in patients with pyogenic spondylitis.201728698035
differentiating staphylococcus aureus from escherichia coli mastitis: s. aureus triggers unbalanced immune-dampening and host cell invasion immediately after udder infection.the etiology determines quality and extent of the immune response after udder infection (mastitis). infections with gram negative bacteria (e.g. escherichia coli) will quickly elicit strong inflammation of the udder, fully activate its immune defence via pathogen receptor driven activation of iκb/nf-κb signaling. this often eradicates the pathogen. in contrast, gram-positive bacteria (e.g. staphylococcus aureus) will slowly elicit a much weaker inflammation and immune response, frequently result ...201728684793
assessing antibiotic therapy effectiveness against the major bacterial pathogens in a hospital using an integrated index.to assess the effectiveness of antibiotic therapy against five indicator bacteria in a chinese hospital using an index-based approach.201728699775
development of silver-nanoparticle-decorated emulsion-templated hierarchically porous poly(1-vinylimidazole) beads for water treatment.water, the driver of nature, has always been polluted by the blind hurling of highly toxic contaminants, but human-friendly science has continuously been presenting better avenues to help solve these challenging issues. in this connection, the present study introduces novel nanocomposites composed of emulsion-templated hierarchically porous poly(1-vinylimidazole) beads loaded with the silver nanoparticles generated via an in situ approach. these nanocomposites have been thoroughly characterized ...201728644011
mie scatter spectra-based device for instant, contact-free, and specific diagnosis of bacterial skin infection.rapid and specific diagnostic techniques are needed to expedite specific treatment of bacterial skin infections with narrow-spectrum antibiotics, rather than broad-spectrum. through this work a device was developed to determine the presence of and species responsible for a bacterial skin infection using differences in mie scatter spectra created by different bacterial species. a 650 nm led at five different incident angles is used to illuminate the tissue, with mie scatter being detected by pin ...201728684745
top-down lesa mass spectrometry protein analysis of gram-positive and gram-negative bacteria.we have previously shown that liquid extraction surface analysis (lesa) mass spectrometry (ms) is a technique suitable for the top-down analysis of proteins directly from intact colonies of the gram-negative bacterium escherichia coli k-12. here we extend the application of lesa ms to gram-negative pseudomonas aeruginosa ps1054 and gram-positive staphylococcus aureus mssa476, as well as two strains of e. coli (k-12 and bl21 mcherry) and an unknown species of staphylococcus. moreover, we demonstr ...201728681361
association of silver nanoparticles and curcumin solid dispersion: antimicrobial and antioxidant properties.the last century, more precisely after 1945, was marked by major advances in the treatment of infectious diseases which promoted a decrease in mortality and morbidity. despite these advances, currently the development of antimicrobial resistance has been growing drastically and therefore there is a pressing need to search for new compounds. silver nanoparticles (agnps) have been demonstrating good antimicrobial activity against different bacteria, viruses, and fungi. curcumin (cur) extracted fro ...201728681332
effect of precursor on antifouling efficacy of vertically-oriented graphene nanosheets.antifouling efficacy of graphene nanowalls, i.e., substrate-bound vertically-oriented graphene nanosheets, has been demonstrated against biofilm-forming gram-positive and gram-negative bacteria. where graphene nanowalls are typically prepared using costly high-temperature synthesis from high-purity carbon precursors, large-scale applications demand efficient, low-cost processes. the advancement of plasma enabled synthesis techniques in the production of nanomaterials has opened a novel and effec ...201728677651
super-cationic carbon quantum dots synthesized from spermidine as an eye drop formulation for topical treatment of bacterial keratitis.we have developed a one-step method to synthesize carbon quantum dots (cqdpas) from biogenic polyamines (pas) as an antibacterial agent for topical treatment of bacterial keratitis (bk). cqds synthesized by direct pyrolysis of spermidine (spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. we demonstrate that cqds obtained from spds (cqdspds) possess effective antibacterial activities against non-multidrug-resista ...201728677399
synthesis, characterization and biological activity of c6-schiff bases derivatives of chitosan.c6-schiff bases derivatives of chitosan were synthesized for the first time. c2-amino groups and c3-hydroxy groups were firstly protected by cuso4·5h2o, and the c6-hydroxy was then transformed into aldehyde, which then reacted with anilines through nucleophilic addition to introduce the cn group at c6-position in chitosan chain. finally, c6-schiff bases derivatives of chitosan were got by the deprotection of c2-nh2 with cation exchange resin. the structures and properties of the new synthesized ...201728673843
screening of ionically crosslinked chitosan-tripolyphosphate microspheres using plackett-burman factorial design for the treatment of intrapocket infections.application of plackett-burman factorial design to investigate the effect of processing factors in the fabrication of ionically crosslinked chitosan-tripolyphosphate (cs-tpp) microspheres.201728673095
a fusion protein of the synthetic igg-binding domain and aequorin: expression and purification from e. coli cells and its application.aequorin is a ca(2+)-binding photoprotein that is a complex of apoaequorin (apoaq) and 2-peroxycoelenterazine. in this study, the fusion protein (zz-apoaq) composed of the synthetic igg-binding domain (zz domain) derived from staphylococcus aureus protein a and apoaq was expressed into the periplasmic space of escherichia coli cells. zz-apoaq was highly purified using ni-chelate affinity chromatography followed by igg affinity chromatography. zz-aq was prepared from purified zz-apoaq by incubati ...201728668497
synthetic scale-up of a novel fluorescent probe and its biological evaluation for surface detection of staphylococcus aureus.this paper reports on the lgx fluorometric test for enzymatic mrsa/mssa detection. it highlights the reasons rhodamines have been overlooked and also strategies to improve the synthesis of rhodamine-peptide conjugates. evaluation of the lgx test for detection of mrsa/mssa on surfaces is undertaken in the presence of potentially confounding e. coli and s. epidermidis for the first time.201728668278
protein-protein interaction: tandem affinity purification in bacteria.the discovery of protein-protein interaction networks can lead to the unveiling of protein complex(es) forming cellular machinerie(s) or reveal component proteins of a specific cellular pathway. deciphering protein-protein interaction networks therefore contributes to a deeper understanding of how cells function. here we describe the protocol to perform tandem affinity purification (tap) in bacteria, which enables the identification of the partners of a bait protein under native conditions. this ...201728667616
a green strategy for the synthesis of sulfone derivatives of p-methylaminophenol: kinetic evaluation and antibacterial susceptibility.this is one of the few examples in which the diverse products have been synthesized just by changing the applied potential. the synthesis of sulfonyl derivatives of p-methylaminophenol were carried out by reaction of the electrogenerated p-methylquinoneimine with sulfinic acids. various types of mono (msp), bis (bsp) and tris (tsp) sulfonyl p-methyl aminophenols were obtained by changing the electrode potential, in one pot under green conditions. the mono sulfonyl-p-(methylamino)phenol derivativ ...201728667267
self-sterilizing sputtered films for applications in hospital facilities.this review addresses the preparation of antibacterial 2d textile and thin polymer films and 3d surfaces like catheters for applications in hospital and health care facilities. the sputtering of films applying different levels of energy led to the deposition of metal/oxide/composite/films showing differentiated antibacterial kinetics and surface microstructure. the optimization of the film composition in regards to the antibacterial active component was carried out in each case to attain the fas ...201728657579
biological activities of allium sativum and zingiber officinale extracts on clinically important bacterial pathogens, their phytochemical and ft-ir spectroscopic analysis.the spread of bacterial infectious diseases is a major public threat. herbs and spices have offered an excellent, important and useful source of antimicrobial agents against many pathological infections. in the current study, the antimicrobial potency of fresh, naturally and commercial dried allium sativum and zingiber officinale extracts had been investigated against seven local clinical bacterial isolates such as escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia, staphylococcus au ...201728653916
anti-inflammatory effect of man-pen-fang, a chinese herbal compound, on chronic pelvic inflammation in rats.traditional chinese medicine (tcm) has become the focus of research for the treatment of chronic pelvic inflammatory disease (cpid) based on unique medical theory system. man-pen-fang (mpf), a chinese herbal compound, which is composed of thlaspi arvense l. (cruciferae), gleditsia sinensis lam. (leguminosae), smilax china l. (liliaceae), euonymus alatus (thunb.) sieb. (celastraceae) and vaccaria segetalis (neck.) (caryophyllaceae) mpf has been used for the treatment of cpid and exerted significa ...201728652014
optimization of hydrogel containing toluidine blue o for photodynamic therapy by response surface methodology.photodynamic therapy with toluidine blue o (tbo) hydrogel exhibits antibacterial activity against staphylococcus aureus and escherichia coli in this paper. the response surface methodology is employed to optimize formulations for antibacterial activity. the optimal formulations are carbomer concentration of 3% (w/v), tbo concentration of 0.1mg/ml and the quality ratio of naoh and carbomer of 0.4 (w/w). under the optimized formulations, the log-transformed of cfuml(-1) on the staphylococcus aureu ...201728649006
staphylococcus aureus strain newman photoinactivation and cellular response to sunlight exposure.sunlight influences microbial water quality of surface waters. previous studies have investigated photoinactivation mechanisms and cellular photostress responses of fecal indicator bacteria (fib), including escherichia coli and enterococci, but further work is needed to characterize photostress responses of bacterial pathogens. here we investigate the photoinactivation of staphylococcus aureus (strain newman), a pigmented, waterborne pathogen of emerging concern. we measured photodecay using sta ...201728646114
bacterial agents and antibiotic resistance profiles of infections from different sites that occurred among patients at debre markos referral hospital, ethiopia: a cross-sectional study.in developing countries like ethiopia, infections with antibiotic resistant bacteria become a real threat. hence, monitoring of local level antimicrobial resistance profile is indispensable to contain the spread of drug resistant bacteria and intervene poor awareness on antimicrobial resistance. therefore, this study aimed at determining bacterial and antibiotic resistance profiles of infections from different sites that occurred among patients.201728683780
carbon nanotubes/carbon fiber hybrid material: a super support material for sludge biofilms.carbon fiber (cf) is widely used as a sludge biofilm support material for wastewater treatment. carbon nanotubes/carbon fiber (cnts/cf) hybrid material was prepared by ultrasonically assisted electrophoretic deposition (epd). cf supports (cf without handling, cf oxidized by nitric acid, cnts/cf hybrid material) were evaluated by sludge immobilization tests, bacterial cell adsorption tests and derjaguin -landau -verwey -overbeek (dlvo) theory. we found that the cnts/cf hybrid material has a high ...201728678624
antimicrobial potential of bacteria associated with marine sea slugs from north sulawesi, indonesia.nudibranchia, marine soft-bodied organisms, developed, due to the absence of a protective shell, different strategies to protect themselves against putative predators and fouling organisms. one strategy is to use chemical weapons to distract predators, as well as pathogenic microorganisms. hence, these gastropods take advantage of the incorporation of chemical molecules. thereby the original source of these natural products varies; it might be the food source, de novo synthesis from the sea slug ...201728659904
antibacterial and antioxidant properties of various solvents extracts of abutilon theophrasti medic. leaves.this paper described the extraction procedure of six extracts from abutilon theophrasti medic. leaves and evaluated antioxidant and antibacterial activity of different extracts by hydroxyl radical, dpph radical scavenging, broth micro-dilution and agar-well diffusion methods. the six extracts were prepared by the two extraction procedures: (i) water was the extraction solvent; (ii) 90% alcohol extract was extracted by petroleum ether, chloroform, ethyl acetate and n-butanol in turn. extract yiel ...201728653920
characterization of phytoconstituents and evaluation of antimicrobial activity of silver-extract nanoparticles synthesized from momordica charantia fruit extract.our present study was conducted to characterize the phytoconstituents present in the aqueous extract of momordica charantia and evaluate the antimicrobial efficacy of silver-extract nanoparticles (ag-extract-nps).201728651578
chimeric analogs of human β-defensin 1 and θ-defensin disrupt pre-established bacterial biofilms.antibiofilm activity of several human defensin analogs that have the ability to kill planktonic bacteria, against pre-established biofilms of escherichia coli mg1655 and staphylococcus aureus nctc 8530 were examined. linear and linear fatty acylated analogs did not show any activity while disulfide constrained analogs disrupted pre-established s. aureus biofilms. chimeric analogs of human β-defensin 1 and θ-defensin, hbtd-1 and [d]hbtd-1 were highly active against s. aureus biofilms. among the a ...201728642103
effect of maternal obesity on maternal-fetal transfer of preoperative cefazolin at cesarean section.american congress of obstetricians and gynecologists recommends a single dose of antibiotic prophylaxis before all cesarean sections (c/s). this recommendation is based on pharmacokinetic studies that include only non-obese patients. we sought to evaluate 1) cefazolin plasma concentrations among obese and non-obese patients after administration of a 2-g cefazolin dose for prevention of surgical wound infections, and 2) whether cefazolin concentration in fetal circulation may be protective agains ...201728638306
synthesis, evaluation and modeling of some triazolothienopyrimidinones as anti-inflammatory and antimicrobial agents.new triazolotetrahydrobenzothienopyrimidinone derivatives were synthesized.201728635307
Displaying items 162701 - 162800 of 163583