Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
phytosynthesis of silver nanoparticles using aqueous leaf extracts of lippia citriodora: antimicrobial, larvicidal and photocatalytic evaluations.nanoscience and nanotechnology represent new and enabling platforms that promise to provide broad range of novel and improved technologies for environmental, biological and other scientific applications. this study reports the synthesis of silver nanoparticles mediated by aqueous leaf extract of lippia citriodora at two different temperatures of 50°c and 90°c. the synthesis of colloidal silver nanoparticles (agnps) was monitored by the use of uv-visible spectroscopy at different temperatures and ...201728415554
effect of variable aminoalkyl chains on chemical grafting of cellulose nanofiber and their antimicrobial activity.recent focus on the preparation of antimicrobial surfaces using cellulose nanofibers (cnf) has gained considerable attention. in this work, functionalization of cnf films in 100% aqueous solution with three different aminosilanes, including 3-aminopropyl trimethoxysilane (apms), 2-aminoethyl 3-aminopropyl trimethoxysilane (dams)and 3-2-(2-aminoethylamino) ethylamino propyl-trimethoxysilane (tams) is reported for the fabrication of contact active antimicrobial materials. grafted cnf films were co ...201728415526
instant green synthesis of silver-based herbo-metallic colloidal nanosuspension in terminalia bellirica fruit aqueous extract for catalytic and antibacterial applications.in the present study, microwave-assisted, optimized, instant, terminalia bellirica fruit extract-mediated green synthesis of colloidal silver nanoparticles (agnps) has been reported. the synthesized agnps were characterized by uv-vis spectroscopy, ftir, zetasizer, fesem, edx and xrd. the characteristic surface plasmon peak of reaction mixture at 406 nm confirmed the synthesis of agnps. the ftir studies confirmed phytoconstituents were responsible for the synthesis and stability of agnps. the fes ...201728409425
a proteomic signature of dormancy in the actinobacterium micrococcus luteus.dormancy is a protective state in which diverse bacteria, including mycobacterium tuberculosis, staphylococcus aureus, treponema pallidum (syphilis), and borrelia burgdorferi (lyme disease), curtail metabolic activity to survive external stresses, including antibiotics. evidence suggests dormancy consists of a continuum of interrelated states, including viable but nonculturable (vbnc) and persistence states. vbnc and persistence contribute to antibiotic tolerance, reemergence from latent infecti ...201728484042
structural and biochemical analyses indicate that a bacterial persulfide dioxygenase-rhodanese fusion protein functions in sulfur assimilation.hydrogen sulfide (h2s) is a signaling molecule that is toxic at elevated concentrations. in eukaryotes, it is cleared via a mitochondrial sulfide oxidation pathway, which comprises sulfide quinone oxidoreductase, persulfide dioxygenase (pdo), rhodanese, and sulfite oxidase and converts h2s to thiosulfate and sulfate. natural fusions between the non-heme iron containing pdo and rhodanese, a thiol sulfurtransferase, exist in some bacteria. however, little is known about the role of the pdo-rhodane ...201728684420
pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty.the aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (optisol™-gs) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in optisol™-gs by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.201728597192
multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts.multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.201728743314
development of an isothermal amplification-based assay for the rapid visual detection of salmonella bacteria.the efficient and timely detection of pathogens is a major concern worldwide. the aim of this study was to establish a rapid detection method for salmonella bacteria in food samples to facilitate timely treatment. widely used detection methods currently include culture-based methods and pcr-based methods. the former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. in this study, a sensiti ...201728711269
a rapid salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay.salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. since conventional culture methods to detect salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. in this study, we developed a rapid, simple and portable salmonella detection strategy that combines thermophilic helicase-dependent amplification (thda) ...201728526589
[effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand].objective: to investigate the effects of vacuum sealing drainage technique in acute and chronic suppurative tenosynovitis of hand. methods: a total of 9 cases acute and chronic suppurative tenosynovitis patients from january 2013 to april 2015 in puai hospital affiliated to tongji medical college, huazhong university of science and technology were retrospectively reviewed. there were 6 males and 3 females, aging from 27 to 65 years, the average age was 55 years. there were 3 cases of index finge ...201728464581
phenanthrenes from arundina graminifolia and in vitro evaluation of their antibacterial and anti-haemolytic properties.chemical investigation and activity test of arundina graminifolia led to the isolation of six phenanthrenes: blestriarene a (1), shancidin (2), densiflorol b (3), ephemeranthoquinone (4), coelonin (5) and lusianthridin (6). the isolated compounds demonstrated antibacterial and anti-haemolytic activities. it was found that compounds 1 and 2 had medium antibacterial activity against staphylococcus aureus, bacillus subtilis and escherichia coli, with mics of 20-40 μg/ml and mbcs of 40-320 μg/ml. ba ...201728553728
introducing a novel mushroom from mycophagy community with emphasis on biomedical potency.mushrooms have been prized by humankind as medicine and culinary wonder since antiquity. though several species are ethnically valued; many prospective species are still being discovered. one such wild macrofungus has recently been discovered during subsequent field surveys in west bengal, india which in turn exposed as a traditionally consumed popular myco-food. the collected taxon was found to be unique with regard to its morphological as well as genetical features. after detailed characteriza ...201728552988
4-substituted thieno[2,3-d]pyrimidines as potent antibacterial agents: rational design, microwave-assisted synthesis, biological evaluation and molecular docking studies.in an attempt to discover a new class of antibacterial agents with improved efficacy and to overcome the drug-resistant problems, some novel 4-substituted thieno[2,3-d]pyrimidines have been synthesized via microwave-assisted methodology and evaluated for their in vitro antibacterial activity against various pathogenic bacterial strains. compounds 12b and 13c showed the promising inhibitory potencies against staphylococcus aureus, bacillus subtilis, pseudomonas aeruginosa and escherichia coli wit ...201728544494
obtaining titanium dioxide nanoparticles with spherical shape and antimicrobial properties using m. citrifolia leaves extract by hydrothermal method.in this work, we synthesized titanium dioxide (tio2) nanoparticles using leaf extract of morinda citrifolia (m. citrifolia) by the advanced hydrothermal method. the synthesized tio2 nanoparticles were characterized by x-ray diffraction (xrd), fourier transmission infrared (ft-ir), ultraviolet-visible diffuse reflectance (uv-vis drs), ultraviolet-visible spectroscopy (uv-vis), raman spectroscopy, and scanning electron microscopy with energy dispersive x-ray spectroscopy (sem with edx) techniques. ...201728501689
bacterial nucleoid occlusion: multiple mechanisms for preventing chromosome bisection during cell division.in most bacteria cell division is driven by the prokaryotic tubulin homolog, ftsz, which forms the cytokinetic z ring. cell survival demands both the spatial and temporal accuracy of this process to ensure that equal progeny are produced with intact genomes. while mechanisms preventing septum formation at the cell poles have been known for decades, the means by which the bacterial nucleoid is spared from bisection during cell division, called nucleoid exclusion (no), have only recently been dedu ...201728500529
synthesis, biological evaluation and molecular modeling study of new (1,2,4-triazole or 1,3,4-thiadiazole)-methylthio-derivatives of quinazolin-4(3h)-one as dhfr inhibitors.a new series of 2-mercapto-quinazolin-4-one analogues was designed, synthesized and evaluated for their in vitro dhfr inhibition, antitumor and antimicrobial activity. compound 17 proved to be the most active dhfr inhibitor with ic50 value of 0.01μm, eight fold more active than methotrexate (mtx). compounds 16 and 24 showed antitumor activity against human caco2 colon and mcf-7 breast tumor cell lines with ic50 values of 25.4 and 9.5μg/ml, respectively. compounds 15, 20, 21 and 30 showed conside ...201728499189
antibacterial activity of isolated immunodominant proteins of naja naja (oxiana) venom.the aim of this study is to investigate antibacterial effects of immunodominant proteins isolated from the venom of naja naja oxiana snake against staphylococcus aureus, escherichia coli, bacillus subtilis and pseudomonas aeruginosa. the innate immune system is an important line of defense against bacterial diseases. antibacterial peptides and proteins produced by snake venoms have recently attracted significant attention due to their relevance to bacterial diseases and the potential of being co ...201728496483
evaluation of pigments from methanolic extract of tagetes erecta and beta vulgaris as antioxidant and antibacterial agent.the total phenolic content (tpc), antioxidant and antibacterial activities of methanolic extract of marigold flower (mf) (tagetes erecta) and beet root (br) (beta vulgaris) were examined. the present work reveals that mf contained greater amount of tpc (42.5 mg/g gae) as compared to br (39.4 mg/g gae). methanolic extract of mf exhibited excellent dpph free radical scavenging power (ic50 0.0716 mg/ml) and reducing power at 1 mg/ml concentration. similar results have been obtained in ftc and tba m ...201728494614
risk of cardiac pacemaker pocket infection in a tertiary care hospital.the risk of pacemaker pocket infections (ppis) is rare with good antisepsis techniques and use of advanced antibiotics. however, injudicious antibiotic usage leads to the rise of multidrug-resistant bacteria, which may cause ppi. few reports exist about the microbial spectrum of the ppi from our country, prompting us to study the same.201728631632
reduction of reactive red 241 by oxygen insensitive azoreductase purified from a novel strain staphylococcus ku898286.an oxygen insensitive azoreductase was purified from a novel bacterial strain (staphylococcus sp. ku898286) that was isolated from an abandoned site of the textile waste discharge unit. the isolated enzyme had efficiently cleaved the azo-bonds through reductive transformation under aerobic conditions. initial phenotypic characterization and final construction of phylogenetic tree on the basis of 16s rdna demonstrated 99% resemblance of the isolate to staphylococcus aureus. the purified azoreduct ...201728467413
endogenous bacterial endophthalmitis. a report of 2cases.the cases are presented on 2patients with bacterial endogenous endophthalmitis. the first one was caused by streptococcus bovis, developed after colonoscopy, which had a poor outcome and resulted in evisceration. the second case was caused by a methicillin resistant staphylococcus aureus from an arthrodesis complicated with a para-spinal abscess. it had an excellent visual outcome.201728427732
[brain abscess in children: ten years of experience in a third level pediatric hospital].brain abscess is a focal infection that occurs with a frequency of 0.3-1.3 cases per 100,000 people/year. we describe the epidemiology, clinical and microbiology characteristics of 38 children diagnosed with brain abscess hospitalized between 4/1/2005 and 12/31/2015 at hospital de pediatría "prof. dr. juan p. garrahan" in buenos aires city. twenty-four patients were male. the median age was 132 months. predisposing factors were detected in 25 children. the median evolution from onset of symptoms ...201728737874
update on the epidemiology and antibiotic resistance of ocular infections.the purpose of this review is to provide an update on the epidemiology and current antibiotic-resistant threats in ophthalmology.201728546690
the changing epidemiology and clinical features of infective endocarditis: a retrospective study of 196 episodes in a teaching hospital in china.infective endocarditis is an uncommon but life-threatening infectious disease. to our knowledge, current investigations of the characteristics of infective endocarditis in our region are scarce. in this study, we aimed to investigate the changes in the epidemiology and clinical features of infective endocarditis.201728482820
bacterial and histopathological findings in deep head and neck infections: a retrospective analysis.deep neck infections are among the most dangerous acute diseases in the head and neck region. this analysis gives an overview of the bacterial and histopathologic findings of deep neck infections.201728411005
derivatives of holomycin and cyclopropaneacetic acid from streptomyces sp. dt-a37.on the basis of the one strain-many compounds strategy, five compounds including two new holomycin derivatives 2 - 3, two new cyclopropaneacetic acid derivatives 4 - 5, together with one known compound holomycin (1) were isolated from a marine-derived bacterium streptomyces sp. dt-a37. their structures were elucidated using nmr and hr-esi-ms analyses. all these compounds were evaluated for their antimicrobial activity, cytotoxic activity, and inhibitory activity against brd4 protein. compound 1 ...201728627048
neo-actinomycins a and b, natural actinomycins bearing the 5h-oxazolo[4,5-b]phenoxazine chromophore, from the marine-derived streptomyces sp. imb094.neo-actinomycins a and b (1 and 2), two new natural actinomycins featuring an unprecedented tetracyclic 5h-oxazolo[4,5-b]phenoxazine chromophore, were isolated from the marine-derived actinomycete streptomyces sp. imb094. their structures were elucidated by spectroscopic analyses. the presence of this ring system was proposed to originate from a condensation between actinomycin d (3) with α-ketoglutarate and pyruvate, respectively. compound 1 showed potent cytotoxic activities against human canc ...201728620204
bioactive bafilomycins and a new n-arylpyrazinone derivative from marine-derived streptomyces sp. hzp-2216e.a meoh extract prepared from culture of an actinomycete streptomyces sp. hzp-2216e isolated from marine green algae ulva pertusa was found to significantly inhibit proliferation of human glioma cells. two different media were applied to culture this marine actinomycete, which produced two new compounds of 23-o-butyrylbafilomycin d and streptoarylpyrazinone a, together with known bafilomycin d, 9-hydroxybafilomycin d, and bafilomycin a1. structures of new compounds were determined by extensive nm ...201728571080
chalcomycins from marine-derived streptomyces sp. and their antimicrobial activities.dihydrochalcomycin (1) and chalcomycin, (2), two known chalcomycins, and chalcomycin e (3), a new compound, were isolated from marine-derived streptomyces sp. hk-2006-1. their structures were elucidated by detailed spectroscopic and x-ray crystallographic analysis. the antimicrobial activities against staphylococcus aureus, escherichia coli, candida albicans, and aspergillus niger of 1-3 were evaluated. compounds 1-2 exhibited activities against s. aureus with minimal inhibitory concentrations ( ...201728555051
isolation, purification, and characterization of five active diketopiperazine derivatives from endophytic streptomyces suk 25 with antimicrobial and cytotoxic activities.in our search for new sources of bioactive secondary metabolites from streptomyces sp., the ethyl acetate extracts from endophytic streptomyces suk 25 afforded five active diketopiperazine (dkp) compounds. the aim of this study was to characterize the bioactive compounds isolated from endophytic streptomyces suk 25 and evaluate their bioactivity against multiple drug resistance (mdr) bacteria such as enterococcus raffinosus, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumanii, p ...201728535606
lobophorin k, a new natural product with cytotoxic activity produced by streptomyces sp. m-207 associated with the deep-sea coral lophelia pertusa.the present article describes the isolation of a new natural product of the lobophorin family, designated as lobophorin k (1), from cultures of the marine actinobacteria streptomyces sp. m-207, previously isolated from the cold-water coral lophelia pertusa collected at 1800 m depth during an expedition to the submarine avilés canyon. its structure was determined using a combination of spectroscopic techniques, mainly esi-tof ms and 1d and 2d nmr. this new natural product displayed cytotoxic acti ...201728534807
streptomyces sp. at37 isolated from a saharan soil produces a furanone derivative active against multidrug-resistant staphylococcus aureus.a novel actinobacterium strain, named at37, showed a strong activity against some multidrug-resistant staphylococcus aureus, including methicillin-resistant s. aureus mrsa atcc 43300, other clinical isolates of mrsa and vancomycin resistant s. aureus vrsa s1. the strain at37 was isolated from a saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. the morphological and chemical studies indicated that this strain belonged to the genus strept ...201728466299
identification of a macrobrachium nipponense c-type lectin with a close evolutionary relationship to vertebrate lectins.c-type lectins (ctls) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. this study cloned and characterized a novel c-type lectin (mnctl) of the oriental river prawn, macrobrachium nipponense. the cloned mnctl cdna encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. the deduced mnctl protein contains a single carbohydrate recognition domain (crd) with an epn (glu-pro-asn) motif in calcium-binding site ...201728441623
a simple way to eradicate methicillin-resistant staphylococcus aureus in cynomolgus macaques (macaca fascicularis).our investigation of indoor-housed cynomolgus macaques (macaca fascicularis) by using automated identification followed by antibiotic susceptibility testing revealed 1 of 7 immunocompetent animals and 2 of 9 immunosuppressed monkeys as carriers of methicillin-resistant staphylococcus aureus (mrsa). follow-up management involving mupirocin treatment resulted in the conversion of the 3 mrsa carriers into mrsa-negative cases. prospective assessment of newly imported monkeys involving 24-h culture o ...201728830583
a simple way to eradicate methicillin-resistant staphylococcus aureus in cynomolgus macaques (macaca fascicularis).our investigation of indoor-housed cynomolgus macaques (macaca fascicularis) by using automated identification followedbyantibiotic susceptibility testing revealed 1 of 7 immunocompetent animals and 2 of 9 immunosuppressed monkeys as carriers ofmethicillin-resistant staphylococcus aureus (mrsa). follow-up management involving mupirocin treatment resulted in the conversion of the 3 mrsa carriers into mrsa-negative cases. prospective assessment of newly imported monkeys involving 24-h culture of n ...201728462776
health and environmental risk assessment project for bottlenose dolphins tursiops truncatus from the southeastern usa. ii. environmental aspects.bottlenose dolphins tursiops truncatus are the most common apex predators found in coastal and estuarine ecosystems along the southeastern coast of the usa, where these animals are exposed to multiple chemical pollutants and microbial agents. in this review, we summarize the results of investigations of environmental exposures evaluated in 360 free-ranging dolphins between 2003 and 2015. bottlenose dolphins inhabiting the indian river lagoon, florida (irl, n = 246), and coastal waters of charles ...201728737160
bacterial prevalence and antibiotic resistance in clinical isolates of diabetic foot ulcers in the northeast of tamaulipas, mexico.diabetic foot ulcers (dfus) are a serious and common problem in patients with diabetes mellitus and constitute one of the major causes of lower extremity amputation. the microbiological profile of dfus depends on the acute or chronic character of the wound. aerobic gram-positive cocci are the predominant organisms isolated from dfus. diabetic foot biopsies from patients admitted to the angiology and vascular surgery hospital of the northeast, in reynosa, tamaulipas from december 2011 to april 20 ...201728682727
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...201728720951
characterization and bioactivities of the exopolysaccharide from a probiotic strain of lactobacillus plantarum wlpl04.exopolysaccharide (eps) was extracted and purified from lactobacillus plantarum wlpl04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. it has a molecular weight of 6.61 × 10(4) da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. microstructural studies demonstrated that the eps appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. the preliminary in vitro assays in ...201728711240
urea-derived graphitic carbon nitride (u-g-c3n4) films with highly enhanced antimicrobial and sporicidal activity.in this manuscript, we describe the fabrication of photoactive biocidal or sporicidal films from urea-derived graphitic carbon nitride (u-g-c3n4). co-deposited films of u-g-c3n4 and escherichia coli o157:h7 (ic50=14.1±0.2mj) or staphylococcus aureus (methicillin resistant ic50=33.5±0.2mj, methicillin sensitive ic50=42.7±0.5mj) demonstrated significantly enhanced bactericidal behavior upon administration of visible radiation (400nm≤λ≤426nm). in all cases, complete eradication of the microbial sam ...201728675870
impacts of pulsed electric field and heat treatment on quality and sensory properties and microbial inactivation of pomegranate juice.synergistic effects of pulsed electric field+mild heat on quality properties of pomegranate juice were modeled using the best-fit multiple (non-) linear regression models with inactivation kinetics parameters of escherichia coli o157:h7 and staphylococcus aureus. no significant difference was detected between the control and the treated samples in terms of ph; °brix; total antioxidant capacity; total monomeric anthocyanin content; total ascorbic acid concentration; and the sensory properties of ...201728610446
sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry.a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ...201728590745
bactericidal effect of 266 to 279nm wavelength uvc-leds for inactivation of gram positive and gram negative foodborne pathogenic bacteria and yeasts.recently, uvc-led technology has been validated as an alternative to irradiation with conventional mercury uv lamps. in this study, we sought to determine primary factors affecting reduction trends shown in several microorganisms. four major foodborne pathogens (escherichia coli o157:h7, salmonella spp. listeria monocytogenes, staphylococcus aureus) and spoilage yeasts (saccharomyces pastorianus, pichia membranaefaciens), important to the brewing industry, were inoculated onto selective and non- ...201728578053
silver nanoparticles: antimicrobial activity, cytotoxicity, and synergism with n-acetyl cysteine.the fast progression of nanotechnology has led to novel therapeutic interventions. antimicrobial activities of silver nanoparticles (ag nps) were tested against standard atcc strains of staphylococcus aureus (atcc 9144), escherichia coli (o157:h7), pseudomonas aeruginosa (atcc 27853), and candida albicans (atcc 90028) in addition to 60 clinical isolates collected from cancer patients. antimicrobial activity was tested by disk diffusion method and mic values for ag nps alone and in combination wi ...201728543603
production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays.in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ...201728536016
[synthesis of antibiotic loaded polylactic acid nanoparticles and their antibacterial activity against escherichia coli o157:h7 and methicillin-resistant staphylococcus aureus].polymeric nanoparticles are promising nanotechnology tools to fight pathogenic bacteria resistant to conventional antibiotics.201728527243
efficacy of (+)-lariciresinol to control bacterial growth of staphylococcus aureus and escherichia coli o157:h7.this study was undertaken to assess the antibacterial potential of a polyphenolic compound (+)-lariciresinol isolated from rubia philippinensis against selected foodborne pathogens staphylococcus aureus kctc1621 and escherichia coli o157:h7. (+)-lariciresinol at the tested concentrations (250 μg/disk) evoked a significant antibacterial effect as a diameter of inhibition zones (12.1-14.9 mm) with minimum inhibitory concentration (mic), and minimum bactericidal concentration values of 125-250 and ...201728515721
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
development of gold nanoparticles coated with silica containing the antibiofilm drug cinnamaldehyde and their effects on pathogenic bacteria.emerging resistance to antibiotics is a mounting worldwide health concern and increases the need for nonantibiotic strategies to combat infectious diseases caused by bacterial pathogens. in this study, the authors used the antibiofilm activity of the naturally occurring antimicrobial cinnamaldehyde (cnma) conjugated to the surface of gold nanoparticles (gnps) to deliver cnma efficiently and eradicate biofilms of gram-negative organisms (enterohemorrhagic escherichia coli o157:h7, and pseudomonas ...201728435260
antibacterial action of jineol isolated from scolopendra subspinipes mutilans against selected foodborne pathogens.this study was undertaken to assess the antibacterial potential of 3,8-dihydroxyquinoline (jineol) isolated from scolopendra subspinipes mutilans against selected foodborne pathogens escherichia coli o157:h7 and staphylococcus aureus kctc-1621. jineol at the tested concentration (50 μl; corresponding to 250 μg/disk) exhibited significant antibacterial effects as a diameter of inhibition zones (11.6-13.6 mm), along with minimum inhibitory concentration (mic) and minimum bactericidal concentration ...201728400770
blood and milk polymorphonuclear leukocyte and monocyte/macrophage functions in naturally caprine arthritis encephalitis virus infection in dairy goats.the exact influence of caprine arthritis encephalitis virus (caev) infection on blood and milk polymorphonuclear leukocytes (pmnls) and monocyte/macrophages of goats remains unclear. thus, the present study sought to explore the blood and milk pmnl and monocyte/macrophage functions in naturally caev-infected goats. the present study used 18 healthy saanen goats that were segregated according to sera test outcomes into serologically caev negative (n=8; 14 halves) and positive (n=10; 14 halves) gr ...201728615124
procalcitonin accurately identifies hospitalized children with low risk of bacterial community-acquired pneumonia.lower procalcitonin (pct) concentrations are associated with reduced risk of bacterial community-acquired pneumonia (cap) in adults, but data in children are limited.201728158460
bacterial genome sequencing in clinical microbiology: a pathogen-oriented review.in recent years, whole-genome sequencing (wgs) has been perceived as a technology with the potential to revolutionise clinical microbiology. herein, we reviewed the literature on the use of wgs for the most commonly encountered pathogens in clinical microbiology laboratories: escherichia coli and other enterobacteriaceae, staphylococcus aureus and coagulase-negative staphylococci, streptococci and enterococci, mycobacteria and chlamydia trachomatis. for each pathogen group, we focused on five di ...201728639162
prophylactic use of intraoperative vancomycin powder and postoperative infection: an analysis of microbiological patterns in 1200 consecutive surgical cases.objective wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. the prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. the purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (ssis) after prophylactic use of vancomycin powder in adult patients under ...201728665245
citrobacter freundii as a cause of acute suppurative thyroiditis in an immunocompetent adult female.acute suppurative thyroiditis (ast) is an uncommon condition, in the patients with preexisting thyroid disease or immunosuppression. the most common cause of ast is bacterial, and the most common bacteria are staphylococcus aureus, staphylococcus epidermidis, escherichia coli, and klebsiella species. due to various complications such as septicemia, septic thrombophlebitis, necrotizing mediastinitis, or pericarditis, prompt diagnosis and treatment are the keys to reduce mortality and morbidity. h ...201728631657
the bactericidal effect of simultaneous titanium oxide on common hospital bacteria.bacteria prevalence has increased in health centers and hospitals. infection control can decrease the number of bacteria. bacteria cause 90% of nosocomial infections and fungi, viruses, or protozoa are less involved. bacteria control can decrease the number of diseases. the simultaneous titanium oxide (smtio) is a new method of disinfection. this process degrades bacteria by producing hydroxyl radical. the present study was conducted with the aim of analyzing the bactericidal effect of simultane ...201728623574
impact of a mixed educational and semi-restrictive antimicrobial stewardship project in a large teaching hospital in northern italy.the overuse of antimicrobials favors the dissemination of antimicrobial resistance, as well as invasive fungal diseases and clostridium difficile infections (cdi). in this study, we assessed the impact of a mixed educational and semi-restrictive antimicrobial stewardship (ams) project in a large teaching hospital in italy.201728856589
infection control measures and prevalence of multidrug-resistant organisms in non-hospital care settings in northeastern germany: results from a one-day point prevalence study.much of the existing literature on the epidemiology of multidrug resistant bacterial organisms (mdros) and infection control measures still concentrates on hospital care settings.201728797758
the potential of alcohol release doorplates to reduce surface contamination during hand contact.optimal hand hygiene may be compromised by contact with contaminated environmental surfaces.201728760635
response to letter of k. singh, 'role of silver nitrate in the efficacy of hydrogen peroxide aerial decontamination systems' regarding s. ali et al. 'efficacy of two hydrogen peroxide vapour aerial decontamination systems for enhanced disinfection of meticillin-resistant staphylococcus aureus, klebsiella pneumoniae and clostridium difficile in single isolation rooms'. 201728734723
clinical characteristics and ultrasonographic findings of acute bacterial enterocolitis in children.this study clarified the bacterial pathogens currently causing acute infectious enterocolitis (aie) in children and evaluated the clinical characteristics and ultrasonographic findings according to the different pathogens.201728730135
risk of surgical site infection, acute kidney injury, and clostridium difficile infection following antibiotic prophylaxis with vancomycin plus a beta-lactam versus either drug alone: a national propensity-score-adjusted retrospective cohort study.the optimal regimen for perioperative antimicrobial prophylaxis is controversial. use of combination prophylaxis with a beta-lactam plus vancomycin is increasing; however, the relative risks and benefits associated with this strategy are unknown. thus, we sought to compare postoperative outcomes following administration of 2 antimicrobials versus a single agent for the prevention of surgical site infections (ssis). potential harms associated with combination regimens, including acute kidney inju ...201728692690
impact of moving to a new hospital build, with a high proportion of single rooms, on healthcare-associated infections and outbreaks.clostridium difficile infection, meticillin-sensitive staphylococcus aureus (mssa) and escherichia coli bacteraemia rates, and bed-days lost during norovirus outbreaks at north bristol nhs trust were analysed over a five-year period to determine whether moving to a new-build hospital with 75% single rooms reduced healthcare-associated infection rates. c. difficile, mssa bacteraemia and e. coli bacteraemia showed no change in the rate of decline after the move. bed-days lost due to norovirus were ...201728666780
the role of antimicrobial sutures in preventing surgical site infection.introduction healthcare associated infections (hcais) are falling following widespread and enforced introduction of guidelines, particularly those that have addressed antibiotic resistant pathogens such as methicillin resistant staphylococcus aureus or emergent pathogens such as clostridium difficile, but no such decline has been seen in the incidence of surgical site infection (ssi), either in the uk, the eu or the us. ssi is one of the hcais, which are all avoidable complications of a surgical ...201728660816
effect of antibiotic stewardship on the incidence of infection and colonisation with antibiotic-resistant bacteria and clostridium difficile infection: a systematic review and meta-analysis.antibiotic stewardship programmes have been shown to reduce antibiotic use and hospital costs. we aimed to evaluate evidence of the effect of antibiotic stewardship on the incidence of infections and colonisation with antibiotic-resistant bacteria.201728629876
crystal structure of the multiple antibiotic resistance regulator marr from clostridium difficile.regulators of multiple antibiotic resistance (marrs) are key players against toxins in prokaryotes. marr homologues have been identified in many bacterial and archaeal species which pose daunting antibiotic resistance issues that threaten public health. the continuous prevalence of clostridium difficile infection (cdi) throughout the world is associated with the abuse of antibiotics, and antibiotic treatments of cdi have limited effect. in the genome of c. difficile strain 630, the marr gene (id ...201728580925
safety and efficacy of antibacterial prophylaxis after craniotomy: a decision model analysis.antibiotic prophylaxis has revolutionized the safety of neurosurgical procedures in the last century. today, the clinician's drug of choice before surgery often is based on the antibiotic's resistance profile and drug-induced complications.201728576711
carboxyl analogue of mutacin 1140, a scaffold for lead antibacterial discovery.mutacin 1140 belongs to the epidermin group of lantibiotics. epidermin class lantibiotics are ribosomally synthesized and posttranslationally modified antibiotics with potent activity against gram-positive bacteria. in particular, this class is effective at targeting drug-resistant streptococcus pneumoniae, methicillin-resistant staphylococcus aureus (mrsa), mycobacterium tuberculosis, and clostridium difficile a c-terminal s-[(z)-2-aminovinyl]-d-cysteine (avicys) residue is derived from a decar ...201728500042
leapfrog hospital safety score, magnet designation, and healthcare-associated infections in united states hospitals.healthcare-associated infections (hais) pose a challenge to patient safety. although studies have explored individual level, few have focused on organizational factors such as a hospital's safety infrastructure (indicated by leapfrog hospital safety score) or workplace quality (magnet recognition). the aim of the study was to determine whether magnet and hospitals with better leapfrog hospital safety scores have fewer hais.201728452915
how well does transfer of bacterial pathogens by culture swabs correlate with transfer by hands?in laboratory testing and in isolation rooms, pickup and transfer of health care-associated pathogens by premoistened rayon swabs correlated well with pickup and transfer by bare hands or moistened gloves. these results suggest that swab cultures provide a useful surrogate indicator of the risk for pathogen pickup and transfer by hands.201728431852
prospective cluster controlled crossover trial to compare the impact of an improved hydrogen peroxide disinfectant and a quaternary ammonium-based disinfectant on surface contamination and health care outcomes.quaternary ammonium-based (quat) disinfectants are widely used, but they have disadvantages.201728431849
colonization by multidrug-resistant organisms in long-term care facilities in italy: a point-prevalence study.to determine prevalence and risk factors for colonization by multidrug-resistant organisms (mdros) in long-term care facility (ltcf) residents in italy. genotypes of mdro isolates were investigated.201728412380
microbiological assessment of honey in méxico.honey is a product used as a natural sweetener and in several regions of mexico and other countries it is also used as a therapeutic agent. microbiological contamination of honey can occur during its extraction and handling. due to the use and consumption of honey we highlighted here the importance of the assessment of its microbiological quality. one thousand nine hundred twenty samples obtained from 8 honey-producing states from mexico were analyzed. from these samples, 40.5% (777/1920) did no ...201728822595
skin infection caused by a novel strain of staphylococcus pseudintermedius in a siberian husky dog owner.introduction.staphylococcus pseudintermedius, an opportunistic pathogen of dogs and cats, is rarely reported to cause infection in humans. here, we describe a case of severe skin infection caused by s. pseudintermedius, in a 47-year-old male, a dog owner; to the best of our knowledge, this is the first such case reported from scotland. case presentation. the patient presented with a short history of a severe ecthyma-like lesion on his forehead, with smaller lesions on his abdomen and legs. bacte ...201728663821
isolation of bacteria from diabetic foot ulcers with special reference to anaerobe isolation by simple two-step combustion technique in candle jar.although polymicrobial infections involving both aerobic and anaerobic bacteria are very common in diabetic foot ulcers, in many centres of developing countries, anaerobes are rarely isolated due to technical difficulties. this can be overcome by using a new simple, innovative technique of a combination of candle combustion and use of acidified copper-coated steel wool, as reported here.201728574021
mycobacterial panniculitis caused by mycobacterium thermoresistibile in a cat.a domestic shorthair cat was evaluated for chronic, bilateral, ulcerative dermatitis affecting the inguinal region and lateral aspects of both pelvic limbs. histopathologic examination of skin biopsies collected throughout the course of disease revealed chronic pyogranulomatous ulcerative dermatitis. aerobic bacterial skin cultures yielded growth of a methicillin-resistant staphylococcus aureus and corynebacterium amycolatum. upon referral the clinical findings were suggestive of a non-tuberculo ...201728491439
test characteristics of milk amyloid a elisa, somatic cell count, and bacteriological culture for detection of intramammary pathogens that cause subclinical mastitis.bovine mastitis is an important disease in the dairy industry, causing economic losses as a result of withheld milk and treatment costs. several studies have suggested milk amyloid a (maa) as a promising biomarker in the diagnosis of mastitis. in the absence of a gold standard for diagnosis of subclinical mastitis, we estimated the diagnostic test accuracy of a commercial maa-elisa, somatic cell count (scc), and bacteriological culture using bayesian latent class modeling. we divided intramammar ...201728647334
impact of intramammary tilmicosin infusion as a dry cow therapy.three hundred subclinically infected quarters of 259 holstein cows infected with gram-positive bacteria were selected via quota sampling based on the california mastitis test (cmt) result and were divided randomly and equally into treatment and test groups. quarters of test group (n = 150 in 128 cows) were treated with an intramammary infusion of tilmicosin, and quarters of the control group (n = 150 in 131 cows) were treated with cloxacillin as a traditional intramammary infusion of dry cow (dc ...201728600854
bacteriological etiology and treatment of mastitis in finnish dairy herds.the finnish dairy herd recording system maintains production and health records of cows and herds. veterinarians and farmers register veterinary treatments in the system. milk samples for microbiological analysis are routinely taken from mastitic cows. the laboratory of the largest dairy company in finland, valio ltd., analyzes most samples using real-time pcr. this study addressed pathogen-specific microbiological data and treatment and culling records, in combination with cow and herd characte ...201728545485
microbiological zoonotic emerging risks, transmitted between livestock animals and humans (2007-2015).as part of the emerging risk identification (eri) activities of the european food safety authority (efsa), a literature search was conducted to identify the microbiological agents transmitted between livestock animals and humans that have been suggested as having emerged between 2007 and 2015 in peer-reviewed scientific literature published during the same period (2007-2015). according to the criteria set, the search identified seven such zoonotic agents, namely west nile fever virus, rift valle ...201728670863
exploitation of spr to investigate the importance of glycan chains in the interaction between lactoferrin and bacteria.bovine lactoferrin (lf) has been shown to prevent adhesion to and invasion of mammalian cell lines by pathogenic bacteria, with evidence for direct bacterial binding by the milk glycoprotein. however, the glycosylation pattern of lf changes over the lactation cycle. in this study, we aim to investigate the effect that this variation has on the milk glycoprotein's ability to interact with pathogens. surface plasmon resonance technology was employed to compare the binding of lf from colostrum (ear ...201728653977
depsidones and an α-pyrone derivative from simpilcillium sp. psu-h41, an endophytic fungus from hevea brasiliensis leaf.nine previously undescribed depsidones (simplicildones a-i) and one previously undescribed α-pyrone (simplicilopyrone) were isolated from the endophytic fungus simplicillium sp. psu-h41 along with 11 known compounds. their structures were established by extensive spectroscopic analysis. simplicildone a and known botryorhodine c displayed weak antibacterial against staphylococcus aureus with equal mic values of 32 μg/ml. additionally, botryorhodine c was active against methicillin-resistant s. au ...201728803995
evaluation of anti-inflammatory and antimicrobial activity of ahpl/aycap/0413 capsule.conventional therapeutic agents used for treatment of acne are associated with various adverse effects necessitating development of safe and effective alternative therapeutic agents. in this context, a polyherbal formulation ahpl/aycap/0413 was developed for treatment of acne.201728827969
conjunctival bacteria flora of glaucoma patients during long-term administration of prostaglandin analog drops.to investigate the effects of the long-term use of prostaglandin analogs for glaucoma treatment on the indigenous flora of the conjunctiva.201728796877
commercial essential oils as potential antimicrobials to treat skin diseases.essential oils are one of the most notorious natural products used for medical purposes. combined with their popular use in dermatology, their availability, and the development of antimicrobial resistance, commercial essential oils are often an option for therapy. at least 90 essential oils can be identified as being recommended for dermatological use, with at least 1500 combinations. this review explores the fundamental knowledge available on the antimicrobial properties against pathogens respo ...201728546822
reduction in bacterial load using hypochlorous acid hygiene solution on ocular skin.to examine the magnitude of bacterial load reduction on the surface of the periocular skin 20 minutes after application of a saline hygiene solution containing 0.01% pure hypochlorous acid (hocl).201728458509
bactericidal potential of silver nanoparticles synthesized using cell-free extract of comamonas acidovorans: in vitro and in silico approaches.the need to overcome human threats from pathogenic microbes, development of nanomaterials have been provoked for a new generation of antimicrobials. in the present study, biosynthesis of silver nanoparticles (agnps) was acquired using comamonas acidovorans extract within 72 h under static condition. electron microscopy studies revealed that the size of agnps was ranging from 6-53 nm and had spherical, oval and irregular shapes with smooth surfaces. prepared agnps interacted with proteins, carboh ...201728555428
eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant: histopathological effects on the zika virus vector aedes aegypti and inhibition of biofilm-forming pathogenic bacteria.the control of zika virus mosquito vectors and well as the development of drugs in the fight against biofilm-forming microbial pathogens, are timely and important challenges in current bionanoscience. here we focused on the eco-friendly fabrication of ag nanostructures using the seed extract of pedalium murex, an ancient indian medicinal plant. initial confirmation of ag nanoparticles (agnps) production was showed by a color change from transparent to dark brown. the uv-visible spectrum (476nm), ...201728772238
participation of 14-3-3ε and 14-3-3ζ proteins in the phagocytosis, component of cellular immune response, in aedes mosquito cell lines.better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. the ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. these proteins have been shown to participate in both humoral and cellular immune responses in drosophila. as mosquitoes of the genus aedes are the primary vectors for arbov ...201728764795
enhanced larvicidal, antibacterial, and photocatalytic efficacy of tio2 nanohybrids green synthesized using the aqueous leaf extract of parthenium hysterophorus.titanium dioxide nanoparticles are emerging as a biocompatible nanomaterial with multipurpose bioactivities. in this study, titanium dioxide (tio2) nanoparticles were effectively synthesized using the aqueous leaf extracts of parthenium hysterophorus prepared by microwave irradiation. tio2 nanoparticles were fabricated by treating the p. hysterophorus leaf extracts with the tio4 solution. biologically active compounds such as alcohols, phenols, alkanes, and fluoroalkanes were involved in bioredu ...201728537028
antiproliferative and antibacterial activities of cirsium scabrum from tunisia.several cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. in the framework of a previous study conducted on eight extremophile plants from tunisia, we highlighted that the crude methanolic extract of c. scabrum, a not investigated thistle, showed moderate but quite selective cytotoxic activity against the cancerous cell line j774 compared to the noncancerous cell line wi38 (ic50 = 11.53 μ ...201728785293
in vitro efficacy of extracts from plants used by small-holder farmers in the treatment of dermatophilosis in cattle.bovine dermatophilosis, an important skin disease of cattle caused by dermatophilus congolensis, negatively impacts the livelihoods of small-holder farmers in zimbabwe. this impact is through, morbidity, loss of draught animal power, costs incurred to manage the disease, losses associated with devalued damaged hides and the resultant culling of some of the affected cattle. due to the inaccessibility of conventional drugs to manage bovine dermatophilosis, farmers have been reported to use local m ...201728573243
hand hygiene practices and microbial investigation of hand contact swab among physiotherapists in an ebola endemic region: implications for public health.hand hygiene practices (hhp), as a critical component of infection prevention/control, were investigated among physiotherapists in an ebola endemic region.201728691027
antibiotic activity of emerimicin iv isolated from emericellopsis minima from talcahuano bay, chile.due to the increasing emergence of resistance of bacterial pathogens to current antibiotics, we have examined the marine fungi present in sea sediments obtained 200 m offshore to discover new antibacterial compounds active against multidrug-resistant bacteria. one strain, identified as emericellopsis minima, was isolated from sediments of talcahuano bay (chile). from the liquid culture of e. minima, we isolated emerimicin iv, a unique fungal peptaibol that exhibited antibacterial activity. the s ...201728669240
antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth.201728748087
introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine.ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ...201728712037
polymicrobial and monomicrobial infections after spinal surgery: a retrospective study to determine which infection is more severe.a retrospective clinical review.201728670411
treatment approach for infection of healed fractures after internal fixation.to review the efficacy of a treatment approach for patients with infection and colonized implants after open reduction and internal fixation of fractures.201728650945
population pharmacokinetics of tigecycline in critically ill patients with severe infections.we sought to describe the population pharmacokinetics of tigecycline in critically ill patients and to determine optimized dosing regimens of tigecycline for different bacterial infections. this prospective study included 10 critically ill patients given a standard dose of tigecycline. blood samples were collected during one dosing interval and were analyzed using validated chromatography. population pharmacokinetics and monte carlo dosing simulations were undertaken using pmetrics. three target ...201728607024
epidemiological, clinical and microbiological characteristics of patients with post-traumatic osteomyelitis of limb fractures in southwest china: a hospital-based study.objective to determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. methods human subject research was performed using institutional review board approved protocols. a retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from january 1, 2012 to d ...201728540152
Refresh
firstprev
...1621162216231624162516261627162816291630...
nextlast
Displaying items 162501 - 162600 of 163583