Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chemical modification and detoxification of the pseudomonas aeruginosa toxin 2-heptyl-4-hydroxyquinoline n-oxide by environmental and pathogenic bacteria. | 2-heptyl-4-hydroxyquinoline n-oxide (hqno), a major secondary metabolite and virulence factor produced by the opportunistic pathogen pseudomonas aeruginosa, acts as a potent inhibitor of respiratory electron transfer and thereby affects host cells as well as microorganisms. in this study, we demonstrate the previously unknown capability of environmental and pathogenic bacteria to transform and detoxify this compound. strains of arthrobacter and rhodococcus spp. as well as staphylococcus aureus i ... | 2017 | 28708374 |
| a new pyranonaphtoquinone derivative, 4-oxo-rhinacanthin a, from roots of indonesian rhinacanthus nasutus. | a new pyranonaphthoquinone derivative, named 4-oxo-rhinacanthin a (1), was isolated from the roots of the indonesian rhinacanthus nasutus together with two known congeners, rhinacanthin a (2) and 3,4-dihydro-3,3-dimethyl-2h-naphtho[2,3-b]pyran-5,10-dione (3). the structure of 1 was elucidated based on its spectroscopic data. the absolute configuration of 1 was assigned by comparing its experimental electronic circular dichroism (ecd) spectrum with the calculated ecd spectrum. compounds 2 and 3 i ... | 2017 | 28566651 |
| preclinical assessment of a (68)ga-dota-functionalized depsipeptide as a radiodiagnostic infection imaging agent. | the study assessed a radiolabeled depsipeptide conjugate ((68)ga-dota-tbia101) for its potential as an imaging agent targeting infection or infection-associated inflammation. (68)ga-labeled dota-tbia101 imaging was performed in (nzr1) healthy rabbits; (nzr2) rabbits bearing muscular sterile inflammation and staphylococcus aureus (sa) infection; and (nzr3) rabbits infected with mycobacterium tuberculosis (mtb) combined with a subcutaneous scruff infection of sa in the same animal. all animals wer ... | 2017 | 28837117 |
| in vitro activity of alpha-viniferin isolated from the roots of carex humilis against mycobacterium tuberculosis. | this study explores the antitubercular activity of α-viniferin, a bioactive phytochemical compound obtained from carex humilis. α-viniferin was active against both drug-susceptible and -resistant strains of mycobacterium tuberculosis at mic50s of 4.6 μm in culture broth medium and mic50s of 2.3-4.6 μm inside macrophages and pneumocytes. in combination with streptomycin and ethambutol, α-viniferin exhibited an additive effect and partial synergy, respectively, against m. tuberculosis h37rv. α-vin ... | 2017 | 28782713 |
| teixobactin and its analogues: a new hope in antibiotic discovery. | increasing bacterial resistance against current antibiotics and lack of new molecules to combat bacterial resistance are key challenges to global health. there is, therefore, a continuing need to develop new antibiotics. teixobactin, a cyclic undecapeptide, displays excellent antibacterial activities against a range of pathogenic bacteria, such as methicillin-resistant staphylococcus aureus (mrsa) and mycobacterium tuberculosis. interestingly, it operates by multiple modes of actions and is bact ... | 2017 | 28770988 |
| plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. | antibiotic resistance is becoming a pivotal concern for public health that has accelerated the search for new antimicrobial molecules from nature. numbers of human pathogens have inevitably evolved to become resistant to various currently available drugs causing considerable mortality and morbidity worldwide. it is apparent that novel antibiotics are urgently warranted to combat these life-threatening pathogens. in recent years, there have been an increasing number of studies to discover new bio ... | 2017 | 28660459 |
| antimicrobial investigation of selected soil actinomycetes isolated from unexplored regions of kashmir himalayas, india. | the aim of the present study was to isolate and evaluate the antimicrobial potential of soil actinomycetes of kashmir himalayas. the secondary metabolites of actinomycetes are the prominent source of antibiotics. a total of 121 morphologically different actinomycete strains were isolated and screened for antimicrobial activity against various human pathogens. the ethyl acetate extract of fermented broth an actinomycete strain, identified as streptomyces pratensis exhibited significant antimicrob ... | 2017 | 28647504 |
| copper and antibiotics: discovery, modes of action, and opportunities for medicinal applications. | copper is a ubiquitous element in the environment as well as living organisms, with its redox capabilities and complexation potential making it indispensable for many cellular functions. however, these same properties can be highly detrimental to prokaryotes and eukaryotes when not properly controlled, damaging many biomolecules including dna, lipids, and proteins. to restrict free copper concentrations, all bacteria have developed mechanisms of resistance, sequestering and effluxing labile copp ... | 2017 | 28528648 |
| pyopericardium with cardiac tamponade in a nigerian child with acute osteomyelitis. | in pyopericardium, pus accumulates in the pericardial space as a result of infection by pyogenic organisms, most common of which are staphylococcus aureus and mycobacterium tuberculosis. these patients are at risk of cardiac tamponade. apart from pericardiocentesis in the management of these patients, definitive drug treatment may pose a formidable challenge in a setting of coinfection as in patients with tuberculosis who are predisposed to secondary bacterial infections. this was the case of ou ... | 2017 | 28465998 |
| endogenous hepcidin and its agonist mediate resistance to selected infections by clearing non-transferrin-bound iron. | the iron-regulatory hormone hepcidin is induced early in infection, causing iron sequestration in macrophages and decreased plasma iron; this is proposed to limit the replication of extracellular microbes, but could also promote infection with macrophage-tropic pathogens. the mechanisms by which hepcidin and hypoferremia modulate host defense, and the spectrum of microbes affected, are poorly understood. using mouse models, we show that hepcidin was selectively protective against siderophilic ex ... | 2017 | 28465342 |
| mycobacterium tuberculosis infection at pacemaker implantation site. | expanding clinical indications of cardiac rhythm management have led to an increased use of pacemaker implantation which is associated with increased incidence of pacemaker infections. staphylococcus aureus and epidermidis account for the majority of pacemaker infections. infection of implantable cardiac devices by mycobacterium species, particularly mycobacterial tuberculosis is rare. we present a case of delayed pacemaker pocket infection with tuberculosis and review of literature of pacemaker ... | 2017 | 28457043 |
| virulence potential of staphylococcus aureus isolates from buruli ulcer patients. | buruli ulcer (bu) is a necrotizing infection of the skin and subcutaneous tissue caused by mycobacterium ulcerans. bu wounds may also be colonized with other microorganisms including staphylococcus aureus. this study aimed to characterize the virulence factors of s. aureus isolated from bu patients. previously sequenced genomes of 21 s. aureus isolates from bu patients were screened for the presence of virulence genes. the results show that all s. aureus isolates harbored on their core genomes g ... | 2017 | 28442219 |
| cathelicidin production and release by mammary epithelial cells during infectious mastitis. | cathelicidins are well-characterized antimicrobial peptides (amps) that are present in significant amounts in mastitic milk. neutrophils are believed to be the main producers of these amps, while the role of mammary epithelial cells (mecs) in their production and release is still unclear. in this work, cathelicidin production patterns were investigated in mammary tissues of ewes infected by staphylococcus aureus, streptococcus uberis, or mycoplasma agalactiae, with a combined approach including ... | 2017 | 28669389 |
| within-herd prevalence of intramammary infection caused by mycoplasma bovis and associations between cow udder health, milk yield, and composition. | subclinical mastitis is one of the major health problems in dairy herds due to decreased milk production and reduced milk quality. the aim of this study was to examine the within-herd prevalence of subclinical intramammary infection caused by mycoplasma bovis and to evaluate associations between m. bovis and cow daily milk yield, udder health, and milk composition. individual cow composite milk samples (n = 522) were collected from all lactating dairy cows in 1 estonian dairy farm in november 20 ... | 2017 | 28601455 |
| synthesis and biological evaluation of novel thioether pleuromutilin derivatives. | to develop new pleuromutilin derivatives as veterinary antibiotic medicines, we designed and synthesized a series of new thioether pleuromutilin derivatives possessing acylthiazolyl moiety based on previously designed derivatives. the antibacterial properties of the prepared pleuromutilin derivatives were assessed in vitro by the broth dilution method against five kinds of bacteria and the mycoplasma mycoplasma gallisepticum (mg). all of the tested compounds displayed moderate to good antibacter ... | 2017 | 28768998 |
| a rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia. | an air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μl reactions without cross-contamination in 45 min. a portable nucleic acid analyzer that integrates mechanical, confocal optical, electronic, and software functions was also developed to collect fluorescence data in a ø3 mm imaging field near the optical diffraction limit for highly s ... | 2017 | 28743917 |
| prevalence of mycoplasma pneumoniae infection in malagasy children. | childhood community-acquired pneumonia is a leading cause of childhood morbidity in low-income countries. the etiologic agents are usually staphylococcus aureus, streptococcus pneumoniae and mycoplasma pneumoniae. m. pneumoniae was recognized as a cofactor in asthmatic disease. high asthma prevalence was reported in madagascar. our aim was to clarify the prevalence of m. pneumoniae infection in this country and its relationship with asthma. | 2017 | 28403048 |
| role of fatty acid kinase in cellular lipid homeostasis and saers-dependent virulence factor expression in staphylococcus aureus. | the saers two-component system is a master activator of virulence factor transcription in staphylococcus aureus, but the cellular factors that control its activity are unknown. fatty acid (fa) kinase is a two-component enzyme system required for extracellular fa uptake and saers activity. here, we demonstrate the existence of an intracellular nonesterified fa pool in s. aureus that is elevated in strains lacking fa kinase activity. saers-mediated transcription is restored in fa kinase-negative s ... | 2017 | 28765222 |
| synthesis and biological evaluation of novel 5-aryl-4-(5-nitrofuran-2-yl)-pyrimidines as potential anti-bacterial agents. | a facile two-step synthetic approach to fluorinated and non-fluorinated 5-aryl-4-(5-nitrofuran-2-yl)-pyrimidines from readily available 5-bromo-4-(furan-2-yl)pyrimidine has been developed. all synthesized compounds were screened in vitro for their antibacterial activities against twelve various bacterial strains. it is demonstrated that some of these compounds exhibited significant antibacterial activities against strains neisseria gonorrhoeae and staphylococcus aureus, comparable and even highe ... | 2017 | 28512023 |
| culture-proven thorn-associated infections in arizona: 10-year experience at mayo clinic. | thorn injuries are common in the desert southwest; however, the frequency and microbiology of thorn-associated infections have not been systematically described. most information comes from case reports describing infections from atypical or environmental microorganisms. our aim was to summarize the spectrum of thorn-associated infections. | 2017 | 28480288 |
| isolation, characterization, virulence and immunogenicity testing of field isolates of pasteurella multocida, staphylococcus aureus, and streptococcus agalactiae in laboratory settings. | the present study was designed to investigate isolation, characterization, virulence and immunogenicity testing of field isolates of pasteurella multocida, staphylococcus aureus, and streptococcus agalactiae in rabbits and mice. isolates of p. multocida, s. aureus and str. agalactiae recovered from field cases of hemorragic septicemia and mastitis were scrutinized for virulence/pathogenicity and immunogenicity. mouse ld50 of p. multocida showed that p. multocida isolate no.1 was more virulent th ... | 2017 | 28450213 |
| cryostored autologous skull bone for cranioplasty? a study on cranial bone flaps' viability and microbial contamination after deep-frozen storage at -80°c. | craniectomy is a life-saving procedure. subsequent cranioplasty with autologous skull bone has a bone resorption rate from 4% to 22.8% and an infection rate from 3.3% to 26%. there are concerns with their viability and the potential microbial contamination as they were explanted for a long period of time. eighteen cranial bone flaps stored at prince of wales hospital skull bone bank during the period from june 2011 to march 2016 were identified. ethics approval was obtained. bone chips and deep ... | 2017 | 28431953 |
| in vitro and in silico antidiabetic and antimicrobial evaluation of constituents from kickxia ramosissima (nanorrhinum ramosissimum). | background and aims:kickxia ramosissima (wall.) janch (or nanorrhinum ramosissimum (wall.) betsche is a well-known medicinal plant in pakistan that is traditionally used in diabetic and inflammatory conditions. because little information is available on its phytochemical composition, a range of constituents were isolated and evaluated in vitro in assays related to the traditional use. methods: dried whole plant material was extracted and chromatographically fractionated. isolated constituents we ... | 2017 | 28507520 |
| microbiome and microbial biofilm profiles of peri-implantitis: a systematic review. | this systematic review assesses the microbiological profiles of peri-implantitis, periodontitis and healthy implants based on studies that evaluated microbial biofilms and entire microbiomes to establish their similarities and differences. | 2017 | 28625077 |
| involvement of cell surface 90 kda heat shock protein (hsp90) in pattern recognition by human monocyte-derived macrophages. | heat shock proteins (hsps) are typical intracellular chaperones which also appear on the cell surface and in extracellular milieu. hsp90, which chaperones many proteins involved in signal transduction, is also a regular component of lps-signaling complexes on mϕ. as lps is a prototypical pamp, we speculated that hsp90 is engaged in pattern recognition by professional phagocytes. in this report, we provide the first evidence, to our knowledge, of the geldanamycin (ge)-inhibitable hsp90 on the sur ... | 2017 | 28550115 |
| septic arthritis of the pubis symphysis: clinical and therapeutic features. | septic arthritis of the pubis symphysis is rare and difficult to diagnose. the objective of our study was to describe the biological, clinical, radiological and therapeutic aspects of this disease. this is a retrospective study of 4 cases of septic arthritis of the pubic symphysis collected in the department of rheumatology and orthopaedics in sousse in tunisia over a period of 16 years (2000-2016). our population consists of 3 women and one men. the mean age was 47 years (18-83). clinical signs ... | 2017 | 28690730 |
| superficial swab versus deep-tissue biopsy for the microbiological diagnosis of local infection in advanced-stage pressure ulcers of spinal-cord-injured patients: a prospective study. | to assess the predictive value of superficial ulcer swab culture to make a microbiological diagnosis of deep wound infections in spinal cord injury (sci) patients with advanced-stage pressure ulcers. | 2017 | 28433727 |
| multipurpose efficacy of zno nanoparticles coated by the crustacean immune molecule β-1, 3-glucan binding protein: toxicity on hepg2 liver cancer cells and bacterial pathogens. | the effective treatment of cancer and bacterial pathogens are two key challenges in modern nanomedicine. here, zinc oxide nanoparticles (zno nps) were fabricated using the crustacean immune molecule β-1, 3- glucan binding protein (phβ-gbp, 100kda) purified from the heamolymph of paratelphusa hydrodromus. β-gbp coated zinc oxide nanoparticles (phβ-gbp-zno nps) were characterized by uv-vis spectroscopy, x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), and high resolution-tr ... | 2017 | 28704712 |
| the risk of cardiac device-related infection in bacteremic patients is species specific: results of a 12-year prospective cohort. | the species-specific risk of cardiac device-related infection (cdri) among bacteremic patients is incompletely understood. | 2017 | 28852678 |
| a broad range quorum sensing inhibitor working through srna inhibition. | for the last decade, chemical control of bacterial virulence has received considerable attention. ajoene, a sulfur-rich molecule from garlic has been shown to reduce expression of key quorum sensing regulated virulence factors in the opportunistic pathogen pseudomonas aeruginosa. here we show that the repressing effect of ajoene on quorum sensing occurs by inhibition of small regulatory rnas (srna) in p. aeruginosa as well as in staphylococcus aureus, another important human pathogen that employ ... | 2017 | 28851971 |
| empirical antibiotic treatment in diabetic foot infection: a study focusing on the culture and antibiotic sensitivity in a population from southern china. | diabetic foot and subsequent diabetic ulcer infections are the most devastating complication of diabetes. this study was conducted to explore the bacterial spectrum, sensitivity of microbials, and analysis of the empirical antibiotic regimens in our health center. the study included patients with diabetic foot ulcer infection (dfi) seen from 2009 to 2014. the patients included had all information covering the physical examination, laboratory tests, and image examinations. we sent appropriately o ... | 2017 | 28836481 |
| novel nitroaniline-cellulose nanohybrids: nitro radical photo-release and its antibacterial action. | in this study, carboxylated nanocrystalline cellulose is surface functionalized by a chromophoric nitro radical (no) photodonor via covalent bonding. the resulting new photoactive nanohybrid material displays an effective photogeneration of no radicals upon illumination by light. the release of no radicals has been chemically detected by various spectroscopic techniques. the bactericidal activity of the novel nanohybrid is effectively demonstrated against both gram positive (staphylococcus aureu ... | 2017 | 28821033 |
| pro- and anti-inflammatory responses of peripheral blood mononuclear cells induced by staphylococcus aureus and pseudomonas aeruginosa phages. | the ability of bacteriophages to kill bacteria is well known, as is their potential use as alternatives to antibiotics. as such, bacteriophages reach high doses locally through infection of their bacterial host in the human body. in this study we assessed the gene expression profile of peripheral blood monocytes from six donors for twelve immunity-related genes (i.e. cd14, cxcl1, cxcl5, il1a, il1b, il1rn, il6, il10, lyz, socs3, tgfbi and tnfa) induced by staphylococcus aureus phage isp and four ... | 2017 | 28808331 |
| axially substituted silicon(iv) phthalocyanine and its quaternized derivative as photosensitizers towards tumor cells and bacterial pathogens. | axially di-(alpha,alpha-diphenyl-4-pyridylmethoxy) silicon(iv) phthalocyanine (3) and its quaternized derivative (3q) were synthesized and tested as photosensitizers against tumor and bacterial cells. these new phthalocyanines were characterized by elemental analysis, and different spectroscopic methods such as ft-ir, uv-vis, maldi-tof and (1)h nmr. the photophysical properties such as absorption and fluorescence, and the photochemical properties such as singlet oxygen generation of both phthalo ... | 2017 | 28807573 |
| oxidative damage to pseudomonas aeruginosa atcc 27833 and staphylococcus aureus atcc 24213 induced by cuo-nps. | the cytotoxicity of nanoparticles (nps) and their properties are important issues in nanotechnology research. particularly, nps affect the metabolism of microorganisms due to np interactions with some biomolecules. in order to assess the mechanisms underlying nps toxicity, we studied the damage caused by copper oxide nanoparticles (cuo-nps) on staphylococcus aureus atcc 24213 and pseudomonas aeruginosa atcc 27833. spherical cuo-nps characterized by their diameter (13 ± 3 nm) were synthesized wit ... | 2017 | 28791555 |
| evaluation of bactericidal and anti-biofilm properties of a novel surface-active organosilane biocide against healthcare associated pathogens and pseudomonas aeruginosa biolfilm. | healthcare acquired infections (hai) pose a great threat in hospital settings and environmental contamination can be attributed to the spread of these. de-contamination and, significantly, prevention of re-contamination of the environment could help in preventing/reducing this threat. goldshield (gs5) is a novel organosilane biocide marketed as a single application product with residual biocidal activity. we tested the hypothesis that gs5 could provide longer-term residual antimicrobial activity ... | 2017 | 28787014 |
| electrophoretic deposition of mgo nanoparticles imparts antibacterial properties to poly-l-lactic acid for orthopedic applications. | bacterial infection of implanted biomaterials is a serious problem that increases health care costs and negatively affects a considerable fraction of orthopedic procedures. in this field, magnesium oxide nanoparticles (mgo nps) have emerged as a promising material to combat bacterial infection while maintaining or improving bone cell functions. here, mgo nps were electrophoretically deposited onto poly-l-lactic acid (plla) sheets to achieve a coating of highly exposed mgo nps that directly influ ... | 2017 | 28782240 |
| regional differences in antibiotic-resistant pathogens in patients with pneumonia: implications for clinicians. | antibiotic resistance is of great concern for both infection control and the treatment of infectious diseases. previous studies reported that the occurrence of drug-resistant pathogens (drps)-for instance, methicillin-resistant staphylococcus aureus (mrsa), pseudomonas aeruginosa and extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae-were associated with inappropriate antibiotic treatment that resulted in adverse outcomes. in addition, unnecessary use of broad-spectrum antibiotics ... | 2017 | 28779516 |
| non-pseudomonal ecthyma gangrenosum caused by methicillin-resistant staphylococcus aureus (mrsa) in a chronic alcoholic patient. | we report for the first time a case of non-pseudomonal ecthyma gangrenosum (eg) caused by methicillin-resistant staphylococcus aureus in a chronic alcoholic without an associated immunocompromised state or disease. eg is an infection of the skin, commonly associated with pseudomonas aeruginosa bacteraemia, though other organisms are also known to be causally related. it usually occurs in patients who are terminally ill and the immunocompromised. in our case, chronic alcoholism with suppression o ... | 2017 | 28775089 |
| evaluation of the antibacterial power and biocompatibility of zinc oxide nanorods decorated graphene nanoplatelets: new perspectives for antibiodeteriorative approaches. | nanotechnologies are currently revolutionizing the world around us, improving the quality of our lives thanks to a multitude of applications in several areas including the environmental preservation, with the biodeterioration phenomenon representing one of the major concerns. | 2017 | 28764786 |
| screening bioactivity and bioactive constituents of nordic unifloral honeys. | the objective of this study was to screen the antibacterial and antioxidant activity of thirty nine honey samples from finland, sweden, norway and denmark. their physicochemical properties were analysed, antioxidant activity was evaluated by dpph assay and antibacterial activity against pseudomonas aeruginosa and staphylococcus aureus was assessed by microdilution assay. the honey samples obtained were buckwheat, caraway, clover, dandelion, fireweed, heather, lime tree, lingonberry, rape, raspbe ... | 2017 | 28763988 |
| development of an accumulation assay and evaluation of the effects of efflux pump inhibitors on the retention of chlorhexidine digluconate in pseudomonas aeruginosa and staphylococcus aureus. | chlorhexidine digluconate (chg) is used as a disinfectant. the emergence of pathogens resistant to the biocide raises health concern. information on specific efflux mechanisms utilised by bacteria to confer reduced susceptibility to the biocide, may be used to develop ways of preventing the efflux of the biocide from nosocomial pathogens resulting in higher disinfection activity. the aim of the study was to evaluate the role of atp-binding cassette transporters on the transport of chg in bacteri ... | 2017 | 28747232 |
| melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities. | geopropolis is a special type of propolis produced by stingless bees. several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from melipona mondury. in this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of m. mondury geopropolis, and determined its chemical profile. the antioxidant activity was determined using in vitro abts·+, ·dpph, and β-carotene/linoleic aci ... | 2017 | 28746550 |
| the detrimental impact of extracellular bacterial proteases on wound healing. | in addition to clinical signs of infection (e.g. inflammation, purulence and pain), a microbial count of ≥10(5) colony-forming units/g has historically been used to define wound infection. however, it is increasingly recognised that, rather than a high bioburden level alone being detrimental to wound healing, it is the virulence of the invading microorganism and the host's immune status that can affect clinical outcomes. bacteria, such as pseudomonas aeruginosa, staphylococcus aureus and staphyl ... | 2017 | 28745010 |
| topical sevoflurane for chronic venous ulcers infected by multi-drug-resistant organisms. | several anaesthetic drugs have demonstrated antibacterial properties in vitro. anaesthetics can primarily affect the cell wall of both susceptible and multi-resistant bacteria. they may also have a synergistic effect with conventional antibiotics through an unknown mechanism. we present three cases of a chronic venous ulcer infected by multi-resistant bacteria refractory to conventional systemic antibiotics, including pseudomonas aeruginosa and methicillin-resistant staphylococcus aureus (mrsa). ... | 2017 | 28736974 |
| antimicrobial resistance in the next 30 years, humankind, bugs and drugs: a visionary approach. | to describe the current standards of care and major recent advances with regard to antimicrobial resistance (amr) and to give a prospective overview for the next 30 years in this field. | 2017 | 28733718 |
| antimicrobial activity of disinfectants commonly used in the food industry in mexico. | disinfectants are widely used in food processing environments for microorganism control; their activity can vary according the microorganism and their used in the appropriated concentrations is vital. hence, the aim of this study was determined the effectiveness and minimum inhibitory concentration (mic) of 15 disinfectants commonly used in the food industry in mexico. | 2017 | 28732782 |
| novel role of surfactant protein a in bacterial sinusitis. | chronic rhinosinusitis (crs) is a common inflammatory disorder of the upper airway characterized by chronic inflammation and significant sinonasal remodeling. crs is comprised of 2 major subgroups, based on whether polyps are present or absent. in some cases, it is characterized by colonization with opportunistic pathogens such as pseudomonas aeruginosa (pa), staphylococcus aureus, and other bacteria. the innate immune system of the sinonasal epithelium is the first line of defense against inhal ... | 2017 | 28727907 |
| antimicrobial activity of selected polyphenols and capsaicinoids identified in pepper (capsicum annuum l.) and their possible mode of interaction. | antimicrobial activity of pepper polyphenols and capsaicinoids (coumarin, caffeic acid, narangin, kaempferol, rutin, quercetin, capsaicin, and dihydrocapsaicin) against 13 pathogen bacteria and three beneficial strains was studied using the disk diffusion and microdilution methods. in general, phenolic compounds had the most important activity with the highest inhibition zones obtained with caffeic acid (3.5-20.5 mm), quercetin (4.75-3.5 mm), and kaempferol (7-14 mm). in the determination of the ... | 2017 | 28721659 |
| pseudomonas aeruginosa alters staphylococcus aureus sensitivity to vancomycin in a biofilm model of cystic fibrosis infection. | the airways of cystic fibrosis (cf) patients have thick mucus, which fosters chronic, polymicrobial infections. pseudomonas aeruginosa and staphylococcus aureus are two of the most prevalent respiratory pathogens in cf patients. in this study, we tested whether p. aeruginosa influences the susceptibility of s. aureus to frontline antibiotics used to treat cf lung infections. using our in vitro coculture model, we observed that addition of p. aeruginosa supernatants to s. aureus biofilms grown ei ... | 2017 | 28720732 |
| frequency of common bacteria and their antibiotic sensitivity pattern in diabetics presenting with foot ulcer. | foot ulcers are one of the most important complications of diabetes mellitus and often lead to lower limb amputation. diabetic foot ulcers are susceptible to infection. the objective of this study was to determine the frequency of common bacteria infecting these ulcers and their antibiotic sensitivity pattern. | 2017 | 28712228 |
| bacterial profile and antibiogram of otitis media among children in yemen. | otitis media is a worldwide disease and is higher in developing countries, particularly among the low socioeconomic levels of the society. the aim of the study is to identify the bacterial etiologic agents of otitis media (om) and their antibiotics sensitivity patterns among children in specialized sam paediatric centre (sspc) and al-mamoon diagnostic medical centre (amdc), in sana'a city. | 2017 | 28712217 |
| retrospective observational study of french patients with cystic fibrosis and a gly551asp-cftr mutation after 1 and 2years of treatment with ivacaftor in a real-world setting. | ivacaftor has been shown to improve lung function and body weight in patients with cf and a gating mutation. real-world evaluation is warranted to examine its safety and effectiveness over the long term. | 2017 | 28711222 |
| intrawound application of vancomycin changes the responsible germ in elective spine surgery without significant effect on the rate of infection: a randomized prospective study. | surgical site infection (ssi) is a costly complication associated with spine surgery. the impact of intrawound vancomycin has not been strongly postulated to decrease the risk of surgical site infection. we designed study to determine whether intrawound vancomycin application reduces the risk of ssi in patients after spine surgery. | 2017 | 28699136 |
| estimating direct cost of cystic fibrosis care using irish registry healthcare resource utilisation data, 2008-2012. | understanding the determinants of cost of cystic fibrosis (cf) care and health outcomes may be useful for financial planning for the delivery of cf services. registries contain information otherwise unavailable to healthcare activity/cost monitoring systems. we estimated the direct medical cost of cf care using registry data and examined how cost was affected by patient characteristics and cf gene (cf transmembrane conductance regulator [cftr]) mutation. | 2017 | 28699086 |
| community composition determines activity of antibiotics against multispecies biofilms. | in young cystic fibrosis (cf) patients, staphylococcus aureus is typically the most prevalent organism, while in adults, pseudomonas aeruginosa is the major pathogen. more recently, it was observed that also streptococcus anginosus plays an important role in exacerbations of respiratory symptoms. these species are often coisolated from cf lungs, yet little is known about whether antibiotic killing of one species is influenced by the presence of others. in the present study, we compared the activ ... | 2017 | 28696232 |
| bioactivity of ceftazidime and fluconazole included in polymethyl methacrylate bone cement for use in arthroplasty. | the microorganisms that most frequently cause prosthetic joint infection are methicillin-resistant staphylococcus aureus and gram-negative aerobic bacillus. studies have documented the efficacy of mixing antibiotics with polymethyl methacrylate, but that of antifungal drugs has not received much attention. the objective of this in vitro study was to characterize the elution profile and bioactivity of ceftazidime and fluconazole when incorporated into bone cement in proportions intended for proph ... | 2017 | 28690040 |
| bactericidal activity of self-assembled palmitic and stearic fatty acid crystals on highly ordered pyrolytic graphite. | the wings of insects such as cicadas and dragonflies have been found to possess nanostructure arrays that are assembled from fatty acids. these arrays can physically interact with the bacterial cell membranes, leading to the death of the cell. such mechanobactericidal surfaces are of significant interest, as they can kill bacteria without the need for antibacterial chemicals. here, we report on the bactericidal effect of two of the main lipid components of the insect wing epicuticle, palmitic (c ... | 2017 | 28688988 |
| effectiveness of topical application of ostrich oil on the healing of staphylococcus aureus- and pseudomonas aeruginosa-infected wounds. | management of infected wounds is one of the major challenges that surgeons and nurses face. several antimicrobial agents have been used, but the toxicity, drug resistance, and their effect on the healing process remain a matter of concern. the present study was designed to analyze the accelerative impact of topical application of ostrich oil on infected wounds in a mouse model. | 2017 | 28682114 |
| antimicrobial ceragenins inhibit biofilms and affect mammalian cell viability and migration in vitro. | the healing of burn wounds is often hampered by bacterial infection and the formation of biofilms. antimicrobial peptides (amps) are effective in promoting wound healing, but are susceptible to degradation. we have tested the ability of ceragenins (csas), mimics of antimicrobial peptides, to mitigate preformed biofilms and stimulate wound healing in vitro. potent csas (mics < 10 μg·ml(-1)) were tested against biofilms formed from a mixture of pseudomonas aeruginosa and staphylococcus aureus grow ... | 2017 | 28680809 |
| activity of innate antimicrobial peptides and ivacaftor against clinical cystic fibrosis respiratory pathogens. | there is a clear need for new antimicrobials to improve current treatment of chronic lung infection in people with cystic fibrosis (cf). this study determined the activities of antimicrobial peptides (amps) and ivacaftor, a novel cf transmembrane conductance regulator potentiator, for cf treatment. antimicrobial activities of amps [ll37, human β-defensins (hβd) 1-4 and slpi] and ivacaftor against clinical respiratory isolates (pseudomonas aeruginosa, staphylococcus aureus, streptococcus spp., ac ... | 2017 | 28666755 |
| properties of induced antimicrobial activity in musca domestica larvae. | insects produce antimicrobial molecules that contribute to their innate immune responses to eliminate invading microorganisms. to explore the potential utility of these antimicrobial molecules, we focused on larvae of the house fly musca domestica, which is an efficient processor of organic waste and a good resource of protein and oil for animal feeding. the induction of hemagglutinating activity, which is usually accompanied by activation of innate immune responses in fly larvae, was observed i ... | 2017 | 28652511 |
| komodo dragon-inspired synthetic peptide drgn-1 promotes wound-healing of a mixed-biofilm infected wound. | cationic antimicrobial peptides are multifunctional molecules that have a high potential as therapeutic agents. we have identified a histone h1-derived peptide from the komodo dragon (varanus komodoensis), called vk25. using this peptide as inspiration, we designed a synthetic peptide called drgn-1. we evaluated the antimicrobial and anti-biofilm activity of both peptides against pseudomonas aeruginosa and staphylococcus aureus. drgn-1, more than vk25, exhibited potent antimicrobial and anti-bio ... | 2017 | 28649410 |
| progress in definition, prevention and treatment of fungal infections in cystic fibrosis. | cystic fibrosis (cf) is a chronic lethal multi-system condition; however, most of the morbidity and mortality is dependent on the status of the respiratory system. progressive respiratory decline is mediated by chronic infection and inflammation, punctuated by important acute events known as pulmonary exacerbations which can lead to accelerated decline. the main bacterial species causing infections include pseudomonas aeruginosa, staphylococcus aureus, haemophilus influenzae and achromobacter xy ... | 2017 | 28762125 |
| air pollution exposure is associated with mrsa acquisition in young u.s. children with cystic fibrosis. | the role of air pollution in increasing susceptibility to respiratory tract infections in the cystic fibrosis (cf) population has not been well described. we recently demonstrated that chronic pm2.5 exposure is associated with an increased risk of initial pseudomonas aeruginosa acquisition in young children with cf. the purpose of this study was to determine whether pm2.5 exposure is a risk factor for acquisition of other respiratory pathogens in young children with cf. | 2017 | 28750627 |
| seasonality of acquisition of respiratory bacterial pathogens in young children with cystic fibrosis. | seasonal variations are often observed for respiratory tract infections; however, limited information is available regarding seasonal patterns of acquisition of common cystic fibrosis (cf)-related respiratory pathogens. we previously reported differential seasonal acquisition of pseudomonas aeruginosa in young children with cf and no such variation for methicillin-susceptible staphylococcus aureus acquisition. the purpose of this study was to describe and compare the seasonal incidence of acquis ... | 2017 | 28599639 |
| comparison of a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform, asta microidsys, with bruker biotyper for species identification. | matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, with its accuracy and speed, is widely used for bacterial identification. the asta microidsys system (asta, korea) was recently developed for species identification. we compared its performance with that of bruker biotyper (bruker daltonics, germany). microbes were recovered from sputum, urine, and pus samples from patients admitted to a tertiary care hospital in korea from january to april 2016. matrix solution (α-cya ... | 2017 | 28840993 |
| application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products. | foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ... | 2017 | 28825446 |
| [distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study]. | objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ... | 2017 | 28810329 |
| efficacy of radiant catalytic ionization to reduce bacterial populations in air and on different surfaces. | air contamination by biological agents is often observed in medical or veterinary facilities and industrial plants. bioaerosols may sediment and pose the surface contamination. microorganisms present on them may become a source of infections among humans and food contamination. this study determined the use of oxidative gases, including ozone and peroxide, generated by the radiant catalytic ionization (rci) cell for the inactivation of acinetobacter baumannii, escherichia coli, enterococcus faec ... | 2017 | 28803189 |
| decontamination of hospital surfaces with multijet cold plasma: a method to enhance infection prevention and control? | objective to evaluate the efficacy of a multijet cold-plasma system and its efficacy in decontaminating 2 surfaces commonly found in hospitals design an in vitro study of common causes of healthcare-acquired infection methods log10 9 cultures of methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, extended spectrum β-lactamase-producing escherichia coli, and acinetobacter baumannii were applied to 5-cm2 sections of stainless steel and mattress. human serum albumin (hsa) ... | 2017 | 28793943 |
| bioprospecting marine actinomycetes for multidrug-resistant pathogen control from rameswaram coastal area, tamil nadu, india. | a potent streptomyces bacillaris strain ram25c4 was isolated for controlling methicillin-resistant staphylococcus aureus and multidrug-resistant bacteria such as staphylococcus aureus, acinetobacter baumannii, and pseudomonas aeruginosa. a total of 131 actinomycetes were isolated from the rameswaram coastal region, tamil nadu, india. among 131 actinomycetes, maximum number of actinomycetes (55%) isolated at the distance of 3-6 m from seashore. out of 131 actinomycetes, 85% of the actinomycetes e ... | 2017 | 28785779 |
| biosynthesized silver nanoparticles: are they effective antimicrobials? | silver nanoparticles (agnps) are increasingly being used in medical applications. therefore, cost effective and green methods for generating agnps are required. | 2017 | 28767978 |
| antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: an in vitro study. | because of increasing antibiotic resistance, herbal teas are the most popular natural alternatives for the treatment of infectious diseases, and are currently gaining more importance. we examined the antimicrobial activities of 31 herbal teas both alone and in combination with antibiotics or antifungals against some standard and clinical isolates of pseudomonas aeruginosa, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae, enterococcus faecalis, methicillin susceptible/resistant s ... | 2017 | 28761777 |
| stability of bacteriophages in burn wound care products. | bacteriophages could be used along with burn wound care products to enhance antimicrobial pressure during treatment. however, some of the components of the topical antimicrobials that are traditionally used for the prevention and treatment of burn wound infection might affect the activity of phages. therefore, it is imperative to determine the counteraction of therapeutic phage preparations by burn wound care products before application in patients. five phages, representatives of two morphologi ... | 2017 | 28750102 |
| a human biofilm-disrupting monoclonal antibody potentiates antibiotic efficacy in rodent models of both staphylococcus aureus and acinetobacter baumannii infections. | many serious bacterial infections are antibiotic-refractory due to biofilm formation. a key structural component of biofilm is extracellular dna which is stabilized by bacterial proteins, including those from the dnabii family. trl1068 is a high affinity human monoclonal antibody against a dnabii epitope conserved across both gram-positive and gram-negative bacterial species. in the current study, the efficacy of trl1068 for disruption of biofilm was demonstrated in vitro in the absence of antib ... | 2017 | 28717038 |
| prioritizing multiple therapeutic targets in parallel using automated dna-encoded library screening. | the identification and prioritization of chemically tractable therapeutic targets is a significant challenge in the discovery of new medicines. we have developed a novel method that rapidly screens multiple proteins in parallel using dna-encoded library technology (elt). initial efforts were focused on the efficient discovery of antibacterial leads against 119 targets from acinetobacter baumannii and staphylococcus aureus. the success of this effort led to the hypothesis that the relative number ... | 2017 | 28714473 |
| bad bacteria in acute appendicitis: rare but relevant. | bacterial infections are a factor for morbidity in patients with acute appendicitis (aa). the spreading of multidrug-resistant (mdr) bacteria is a significant problem in surgery, and the most relevant mdr pathogens are summarized as enterobacteriaceae, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterococci (eskape) bacteria. data regarding the species and distribution of bacteria in aa are available, but information about the resistances an ... | 2017 | 28710611 |
| the clinical and microbiological characteristics of infections in burn patients from the formosa fun coast dust explosion. | bloodstream infection is a leading cause of mortality among burn patients. this study aimed to evaluate the risk factors, causative pathogens, and the relationship between bloodstream infections and other infections among burn patients from the formosa fun coast dust explosion. | 2017 | 28705768 |
| development of silver nanoparticles loaded chitosan-alginate constructs with biomedical potentialities. | herein, a facile biosynthesis of silver nanoparticles (agnps) and agnps-loaded chitosan-alginate constructs with biomedical potentialities is reported. the uv-vis spectroscopic profile confirmed the synthesis of agnps using methanolic leaves extract of euphorbia helioscopia. the newly developed agnps were characterized using various analytical and imaging techniques including uv-vis and ft-ir spectroscopy, x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive spectroscop ... | 2017 | 28705499 |
| antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial. | the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study. | 2017 | 28701170 |
| investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
| titanium surfaces immobilized with the major antimicrobial fragment fk-16 of human cathelicidin ll-37 are potent against multiple antibiotic-resistant bacteria. | infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. previously, the authors' laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17biphe2), a peptide engineered based on the only human cathelicidin. this study documen ... | 2017 | 28675109 |
| targeted surveillance and infection-related risk factors of nosocomial infection in patients after neurosurgical operation. | the purpose of this study was to investigate the risk factors of nosocomial infection in patients after neurosurgical operations, so as to provide reasonable, effective preventative measures in the future. a total of 1,600 patients with brain tumors, hydrocephalus, craniocerebral trauma and vascular disease treated in the neurosurgery room were chosen for targeted surveillance; and through analysis on the clinical data of patients, the incidence of nosocomial infection, the site of infection and ... | 2017 | 28671080 |
| nonribosomal peptide synthetase biosynthetic clusters of eskape pathogens. | covering: up to 2017.natural products are important secondary metabolites produced by bacterial and fungal species that play important roles in cellular growth and signaling, nutrient acquisition, intra- and interspecies communication, and virulence. a subset of natural products is produced by nonribosomal peptide synthetases (nrpss), a family of large, modular enzymes that function in an assembly line fashion. because of the pharmaceutical activity of many nrps products, much effort has gone in ... | 2017 | 28642945 |
| the anticancer peptide tat-rasgap317-326 exerts broad antimicrobial activity. | antibiotic resistance has become a major health issue. nosocomial infections and the prevalence of resistant pathogenic bacterial strains are rising steadily. therefore, there is an urgent need to develop new classes of antibiotics effective on multi-resistant nosocomial pathogenic bacteria. we have previously shown that a cell-permeable peptide derived from the p120 ras gtpase-activating protein (rasgap), called tat-rasgap317-326, induces cancer cell death, inhibits metastatic progression, and ... | 2017 | 28638371 |
| role of positively charged residues on the polar and non-polar faces of amphipathic α-helical antimicrobial peptides on specificity and selectivity for gram-negative pathogens. | we have designed de novo and synthesized eight 26-residue all d-conformation amphipathic α-helical cationic antimicrobial peptides (amps), four with "specificity determinants" which provide specificity for prokaryotic cells over eukaryotic cells and four amps without specificity determinants. the eight amps contain six positively charged lys residues on the polar face in four different arrangements to understand the role of these residues have on antimicrobial activity against 14 acinetobacter b ... | 2017 | 28636788 |
| identification of 1-((2,4-dichlorophenethyl)amino)-3-phenoxypropan-2-ol, a novel antibacterial compound active against persisters of pseudomonas aeruginosa. | antibiotics typically fail to completely eradicate a bacterial population, leaving a small fraction of transiently antibiotic-tolerant persister cells intact. persisters are therefore seen to be a major cause of treatment failure and greatly contribute to the recalcitrant nature of chronic infections. the current study focused on pseudomonas aeruginosa, a gram-negative pathogen belonging to the notorious eskape group of pathogens (enterococcus faecium, staphylococcus aureus, klebsiella pneumonia ... | 2017 | 28630188 |
| microbial characteristics of nosocomial infections and their association with the utilization of hand hygiene products: a hospital-wide analysis of 78,344 cases. | nosocomial infections are the main adverse events during health care delivery. hand hygiene is the fundamental strategy for the prevention of nosocomial infections. microbial characteristics of nosocomial infections in the asia-pacific region have not been investigated fully. correlation between the use of hand hygiene products and the incidence of nosocomial infections is still unknown. this study investigates the microbial characteristics of nosocomial infections in the asia-pacific region and ... | 2017 | 28613991 |
| patterns of multi-drug resistant bacteria at first culture from patients admitted to a third level university hospital in calabria from 2011 to 2014: implications for empirical therapy and infection control. | surveillance of antimicrobial drug resistance is fundamental to guide empirical treatment. however, the european antimicrobial resistance surveillance network provides a general picture, which might not be applicable to clinical settings that are excluded from this survey. we evaluated resistance patterns of eskape isolates over a four-year period in a third level university hospital in the province of catanzaro (southern italy). in this retrospective study, we evaluated the frequency of eskape ... | 2017 | 28603227 |
| antimicrobial activity, toxicity and anti-inflammatory potential of methanolic extracts of four ethnomedicinal plant species from punjab, pakistan. | the plant species aristolochia indica (ai), melilotus indicus (mi), tribulus terrestris (tt) and cuscuta pedicellata (cp) are widely used in folk medicine in the villages around chowk azam, south punjab, pakistan. the aim of this study was to evaluate the antioxidant activity, phytochemical composition, and the antibacterial, antifungal, cytotoxic and anti-inflammatory potential of the four medicinal plants listed above. for cp stem, this study represents (to the best of our knowledge) the first ... | 2017 | 28595608 |
| prevalence of biofilm producing aerobic bacterial isolates in burn wound infections at a tertiary care hospital in northern india. | burn wounds frequently get infected due to a break in skin integrity and prolonged hospitalization. microbial flora originating from the patient's own flora colonize and infect the burn wounds. bacterial biofilms in particular are postulated as the culprit for the development of non-healing burn wounds by inducing chronic inflammation in these patients. in the present study, 190 wound isolates obtained from patients admitted to the burn ward at the pt. b.d. sharma pgims, rohtak, were evaluated f ... | 2017 | 28592933 |
| evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms. | scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus). | 2017 | 28587870 |
| bacteria from animals as a pool of antimicrobial resistance genes. | antimicrobial agents are used in both veterinary and human medicine. the intensive use of antimicrobials in animals may promote the fixation of antimicrobial resistance genes in bacteria, which may be zoonotic or capable to transfer these genes to human-adapted pathogens or to human gut microbiota via direct contact, food or the environment. this review summarizes the current knowledge of the use of antimicrobial agents in animal health and explores the role of bacteria from animals as a pool of ... | 2017 | 28587316 |
| properties of probiotics kocuria sm1 and rhodococcus sm2 isolated from fish guts. | this study characterized probiotics kocuria sm1 and rhodococcus sm2, which were recovered from the intestinal microbiota of rainbow trout (oncorhynchus mykiss, walbaum). the cultures were gram-positive, non-motile, catalase-positive and oxidase-negative cocci or rods. cell multiplication of sm1 and sm2 was observed at 4-37 °c (45 °c for sm1), in 0-20% (w/v) nacl and at ph 2-11. the viability was not affected when exposed to pepsin at ph 2.0 and 3.0, and pancreatin at ph 8.0. neither isolates wer ... | 2017 | 28585158 |
| transmission of health care-associated infections from roommates and prior room occupants: a systematic review. | pathogens that cause health care-associated infections (hais) are known to survive on surfaces and equipment in health care environments despite routine cleaning. as a result, the infection status of prior room occupants and roommates may play a role in hai transmission. we performed a systematic review of the literature evaluating the association between patients' exposure to infected/colonized hospital roommates or prior room occupants and their risk of infection/colonization with the same org ... | 2017 | 28579835 |
| design of live attenuated bacterial vaccines based on d-glutamate auxotrophy. | vaccine development is a priority for global health due to the growing multidrug resistance in bacteria. d-glutamate synthesis is essential for bacterial cell wall formation. here we present a strategy for generating effective bacterial whole-cell vaccines auxotrophic for d-glutamate. we apply this strategy to generate d-glutamate auxotrophic vaccines for three major pathogens, acinetobacter baumannii, pseudomonas aeruginosa and staphylococcus aureus. these bacterial vaccines show virulence atte ... | 2017 | 28548079 |
| celecoxib enhances the efficacy of low-dose antibiotic treatment against polymicrobial sepsis in mice and clinical isolates of eskape pathogens. | treatment of multidrug resistant bacterial infections has been a great challenge globally. previous studies including our study have highlighted the use of celecoxib, a non-steroidal anti-inflammatory drug in combination with antibiotic has decreased the minimal inhibitory concentration to limit staphylococcus aureus infection. however, the efficacy of this combinatorial treatment against various pathogenic bacteria is not determined. therefore, we have evaluated the potential use of celecoxib i ... | 2017 | 28533769 |
| antimicrobial consumption, costs and resistance patterns: a two year prospective study in a romanian intensive care unit. | due to the vulnerable nature of its patients, the wide use of invasive devices and broad-spectrum antimicrobials used, the intensive care unit (icu) is often called the epicentre of infections. in the present study, we quantified the burden of hospital acquired pathology in a romanian university hospital icu, represented by antimicrobial agents consumption, costs and local resistance patterns, in order to identify multimodal interventional strategies. | 2017 | 28532467 |
| biofilm is a major virulence determinant in bacterial colonization of chronic skin ulcers independently from the multidrug resistant phenotype. | bacterial biofilm is a major factor in delayed wound healing and high levels of biofilm production have been repeatedly described in multidrug resistant organisms (mdros). nevertheless, a quantitative correlation between biofilm production and the profile of antimicrobial drug resistance in delayed wound healing remains to be determined. microbial identification, antibiotic susceptibility and biofilm production were assessed in 135 clinical isolates from 87 patients. gram-negative bacteria were ... | 2017 | 28513576 |