Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
predictive factors of methicillin-resistant staphylococcus aureus infection in elderly patients with community-onset pneumonia.methicillin-resistant staphylococcus aureus (mrsa) infection is a severe and life-threatening disease in patients with community-onset (co) pneumonia. however, the current guidelines lack specificity for a screening test for mrsa infection.201728416961
[clinical characteristics and prognosis of cultured negative pyogenic spondylitis].there are limited data describing the clinical characteristics and prognosis of culture negative pyogenic spondylitis. the aim of this study was to investigate the treatment, prognosis and clinical characteristics of culture negative pyogenic spondylitis.201728416829
comparative pharmacodynamics of telavancin and vancomycin in the neutropenic murine thigh and lung infection models against staphylococcus aureus.the pharmacodynamics of telavancin and vancomycin were compared using neutropenic murine thigh and lung infection models. four staphylococcus aureus strains were included. the telavancin mic ranged from 0.06 to 0.25 mg/liter, and the vancomycin mic ranged from 1 to 4 mg/liter. the plasma pharmacokinetics of escalating doses (1.25, 5, 20, and 80 mg/kg of body weight) of telavancin and vancomycin were linear over the dose range. epithelial lining fluid (elf) pharmacokinetics for each drug revealed ...201728416551
ai-2 quorum sensing negatively regulates rbf expression and biofilm formation in staphylococcus aureus.staphylococcus aureus is an important pathogen that is capable of forming biofilms on biomaterial surfaces to cause biofilm-associated infections. autoinducer 2 (ai-2), a universal language for interspecies communication, is involved in a variety of physiological activities, although its exact role in gram-positive bacteria, especially in s. aureus, is not yet thoroughly characterized. herein we demonstrate that inactivation of luxs, which encodes ai-2 synthase, resulted in increased biofilm for ...201728416278
luteolin reduces inflammation in staphylococcus aureus-induced mastitis by inhibiting nf-kb activation and mmps expression.mastitis is a serious and prevalent disease caused by infection by pathogens such as staphylococcus aureus. we evaluated the anti-inflammatory effects and mechanism of luteolin, a natural flavonoid with a wide range of pharmacological activities, in a mouse model of s. aureus mastitis. we also treated cultured mouse mammary epithelial cells (mmecs) with s. aureus and luteolin. histopathological changes were examined by h&e staining and the levels of inflammatory cytokine proteins were analyzed u ...201728415707
antimicrobial property, cytocompatibility and corrosion resistance of zn-doped zro2/tio2 coatings on ti6al4v implants.zn-doped zro2/tio2 porous coatings (zn-zro2/tio2) were prepared on the surface of titanium alloy (ti6al4v) by a hybrid approach of magnetron sputtering and micro-arc oxidation (mao). the microstructures, phase constituents and elemental states of the coating were investigated by scanning electron microscope (sem) equipped with energy dispersive spectroscopy (eds), x-ray diffraction (xrd), and x-ray photoelectron spectroscopy (xps). the results demonstrate that the zn-zro2/tio2 coatings are porou ...201728415518
the bactericidal mechanism of action against staphylococcus aureus for ago nanoparticles.to identify the mechanistic effects of ago nanoparticles on gram-positive bacteria, s. aureus cells suspended in phosphate buffer solution (pbs) and deionized water were separately treated using ago nanoparticles at different concentrations. the phase composition changes of the bactericide after killing s. aureus and the cellular responses of s. aureus to ago were characterized by x-ray diffraction, atomic absorption spectrophotometer, scanning electron microscopy, transmission electron microsco ...201728415506
role of silver/zinc oxide in affecting de-adhesion strength of staphylococcus aureus on polymer biocomposites.a single-cell force spectroscopy is utilized to characterize the dynamics of the transient interaction (0-10s) between alive staphylococcus aureus and ultra-high molecular weight polyethylene (uhmwpe). adhesion force of bacteria upon addition of antibacterial additives (i.e. silver (ag) nanoparticles and zinc oxide (zno) micro-rods) has been evaluated. addition of ag and zno reduces the colony forming units (cfus) from 93±7 (uhmwpe) to 23±5 (u+3wt.% ag+3wt.% zno) on 10×10mm(2) area of sample sur ...201728415396
antibacterial, osteogenic, and angiogenic activities of srtio3 nanotubes embedded with ag2o nanoparticles.biomedical titanium (ti) implants with good anti-infective, osteogenic, and angiogenic properties are in great demand. srtio3 nanotubes (nts) are embedded with silver oxide (ag2o) nanoparticles (nps) (denoted as nt-sr-ag) by a hydrothermal treatment of tio2 nts containing ag2o nps (denoted as nt-ag) in a sr(oh)2 solution. the morphology, composition, microstructure, ion release phenomenon, as well as antibacterial, osteogenic, and angiogenic activities are investigated in details. during the hyd ...201728415389
the model for end-stage liver disease (meld) as a predictor of short-term mortality in staphylococcus aureus bloodstream infection: a single-centre observational study.automated laboratory-based prediction models may support clinical decisions in staphylococcus aureus bloodstream infections (bsis), which carry a particularly high mortality. small studies indicated that the laboratory-based model for end-stage liver disease (meld) score is a risk factor for mortality in critically ill patients with infections. for s. aureus bsis, we therefore aimed to assess a potential association of the meld score with mortality.201728414786
a rare case of tropical pyomysitis acquired on vacation in a healthy male.pyomyositis has traditionally been considered a tropical disease. increasing prevalence has been observed in more northern climates, especially with hiv, immunosuppression, and iv drug abuse as predisposing factors. pyomyositis has the tendency to mimic other conditions, such as muscle contusion, necrotizing fasciitis, or septic arthritis. the rarity of this disease and non-specificity of its symptoms often lead to delay in appropriate diagnosis and treatment.201728414677
evaluation and application of molecular genotyping on nosocomial pathogen-methicillin-resistant staphylococcus aureus isolates in guangzhou representative of southern china.staphylococcus aureus is recognized as a typical food-born pathogen in food safety, and its rapid detection and typing methods are in desperate need. to study their evolution characteristics and drug resistance condition, staphylococci isolates were subject to the investigation. strain identification were subjected to standard procedures (colony morphology, gram staining, vitek 2 automated system and the api commercial kit), and fingerprinting was then performed with rapd-pcr amplification. in t ...201728414166
the mechanism of human β-defensin 3 in mrsa-induced infection of implant drug-resistant bacteria biofilm in the mouse tibial bone marrow.the mechanism of human β-defensin 3 (hbd-3) in methicillin-resistant staphylococcus aureus (mrsa-induced infection of implant drug-resistant bacteria biofilm in the mouse tibial bone marrow was studied. healthy adult male sprague-dawley rats with average weight of 230 g were selected to construct the infection model of mrsa-induced implant drug-resistant bacteria biofilm in the mouse left tibial bone marrow. the drugs were intraperitoneally injected after 24 h medullary cavity infection, and the ...201728413476
rate of contamination of hospital privacy curtains on a burns and plastic surgery ward: a cross-sectional study.surfaces in the patient environment may play a role in microbial transmission if they become colonized by bacteria. patient privacy curtains are one such surface that may pose a high risk for transmission because they are high-contact surfaces, are infrequently cleaned, and healthcare workers are less likely to wash their hands after contacting inanimate objects such as curtains.201728413115
[antibiotic susceptibility of staphylococcus aureus and streptococcus pneumoniae in healthy carrier individuals in primary care in barcelona area].the information available on antibiotic resistance patterns are generally based on specimens from hospitalised individuals. this study was aimed at evaluating the antibiotic resistance rate of nasal carriage strains of staphylococcus aureus and streptococcus pneumoniae in healthy individuals, in accordance with age and gender, attended in primary care centres (pcc).201728413102
does a quality improvement campaign accelerate take-up of new evidence? a ten-state cluster-randomized controlled trial of the institute for health improvement's project joints.a decade ago, the institute for healthcare improvement pioneered a quality improvement (qi) campaign, leveraging organizational and personal social networks to disseminate new practices. there have been few rigorous studies of the qi campaign approach.201728412954
a facile, rapid and sensitive detection of mrsa using a crispr-mediated dna fish method, antibody-like dcas9/sgrna complex.rapid and reliable diagnosis of methicillin-resistant staphylococcus aureus (mrsa) is crucial for guiding effective patient treatment and preventing the spread of mrsa infections. nonetheless, further simplification of mrsa detection procedures to shorten detection time and reduce labor relative to that of conventional methods remains a challenge. here, we have demonstrated a clustered regularly interspaced palindromic repeats (crispr)-mediated dna-fish method for the simple, rapid and highly se ...201728412663
first report on mrsa cc398 recovered from wild boars in the north of portugal. are we facing a problem?the aim of the present study was to evaluate the resistance of staphylococcus aureus recovered from wild boars, to analyze their genetic lineages, and to investigate the susceptibility to oxacillin. samples from mouth and nose of 45 wild boars (sus scrofa) were collected during hunt activity from november 2012 to january 2013 in the north of portugal. s. aureus isolates were recovered from 30 of these samples (33%); one isolate/sample was further studied. the susceptibility of the isolates was t ...201728412568
universal screening and decolonization for control of mrsa in nursing homes: follow-up of a cluster randomized controlled trial.in 2010-11, a trial conducted in nursing homes showed no benefit of meticillin-resistant staphylococcus aureus (mrsa) universal screening and decolonization over standard precautions to reduce the prevalence of mrsa carriage. accordingly, no routine screening was performed from 2012. a five-year follow-up shows no new evidence supporting the intervention. recommendations issued after trial (no screening and decolonization of mrsa residents) were retained.201728412178
screening nasal swabs for methicillin resistant staphylococcus aureus: a regional burn center's experience.methicillin-resistant staphylococcus aureus (mrsa) is a pathogen that can result in substantial morbidity and mortality. early detection of mrsa colonization by screening nasal swabs may be important in the management of burn-injured patients. however, studies examining its use in this population are limited. the aim of this study was to study the utility of admission mrsa screening nasal swabs and determine if being positive for mrsa on admission impacted outcomes.201728412131
effect of antimicrobial and physical treatments on growth of multispecies staphylococcal biofilms.the prevalence and structure of staphylococcus aureus and staphylococcus epidermidis within multispecies biofilms were found to depend sensitively on physical environment and antibiotic dosage. although these species commonly infect similar sites, such as orthopedic implants, little is known about their behavior in multispecies communities, particularly in response to treatment. this research establishes that s. aureus is much more prevalent than s. epidermidis when simultaneously seeded and gro ...201728411222
construction of a gene knockdown system based on catalytically inactive ("dead") cas9 (dcas9) in staphylococcus aureus.there has been an absence of an efficient method of gene knockdown in the important human pathogen staphylococcus aureus like rna interference in eukaryotes. the previously developed antisense rna technology is mainly applied for forward genetic screening but is rather limited in specific gene knockdown because of the lack of rational antisense rna design strategies. here we report an efficient and specific system for gene knockdown in s. aureus based on the type ii clustered regularly interspac ...201728411216
direct antimicrobial activity of ifn-β.type i ifns are a cytokine family essential for antiviral defense. more recently, type i ifns were shown to be important during bacterial infections. in this article, we show that, in addition to known cytokine functions, ifn-β is antimicrobial. parts of the ifn-β molecular surface (especially helix 4) are cationic and amphipathic, both classic characteristics of antimicrobial peptides, and we observed that ifn-β can directly kill staphylococcus aureus further, a mutant s. aureus that is more se ...201728411186
antibacterial efficacy of silver nanoparticles against multi-drug resistant clinical isolates from post-surgical wound infections.in order to investigate new effective and inexpensive nano-therapeutic approach for p. aeruginosa, staphylococcus aureus and coagulase negative staphylococci (cons), the present study reports an eco-friendly process for rapid synthesis of silver nanoparticles (ag-nps) using aqueous leaf extract of corchorus capsularis (crcp). formation of stable ag-nps at different time intervals gives mostly spherical particles with diameters ranging from 5 to 45 nm. the resulting ag-nps were characterized usin ...201728411059
wearing id badges in the operating room environment: is reconsideration warranted?surgical site infection and nosocomial infections in general have appropriately undergone increased scrutiny over the last decade. numerous studies have documented pathogenic bacterial contamination of personal items such as cell phones, pagers, ties, and pens in the hospital setting. it is our understanding that joint commission on accreditation of healthcare organizations requires all personnel to wear an identification badge at all times, which includes the operating room environment.201728410834
staphylococcus aureus surface contamination of mobile phones and presence of genetically identical strains on the hands of nursing personnel.we investigated the genetic relatedness of staphylococcus aureus isolates recovered from mobile phones and palms and fingers of users. genetically identical isolates were detected from mobile phones and their user and multiple users, which is consistent with mobile phones serving as reservoirs of infection in the health care environment. these findings reinforce the need for hand hygiene prior to patient contact as the most effective intervention for preventing health care-associated infection.201728410827
deconstructing the relative benefits of a universal glove and gown intervention on mrsa acquisition.the 20-site benefits of universal glove and gown (bugg) study found that wearing gloves and gowns for all patient contacts in the intensive care unit (icu) reduced acquisition rates of meticillin-resistant staphylococcus aureus (mrsa). the relative importance of gloves and gowns as a barrier, improved hand hygiene, and reduced healthcare worker (hcw)-patient contact rates is unknown.201728410760
accessory gene regulator (agr) functionality in staphylococcus aureus derived from lower respiratory tract infections.characterization of staphylococcus aureus clinical isolates derived from lower respiratory tract infections (lrtis), and correlation between the functionality of the accessory gene regulator (agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome.201728410390
ccr-2 neutralization augments murine fresh bmc activation by staphylococcus aureus via two distinct mechanisms: at the level of ros production and cytokine response.ccr-2 signaling regulates recruitment of monocytes from the bone marrow into the bloodstream and then to sites of infection. we sought to determine whether ccl-2/ccr-2 signaling is involved in the killing of staphylococcus aureus by murine bone marrow cells (bmcs). the intermittent link of reactive oxygen species (ros)-nf-κb/p38-mapk-mediated ccl-2 production in ccr-2 signaling prompted us to determine whether neutralization of ccr-2 augments the response of murine fresh bmcs (fbmcs) after s. au ...201728409543
hospital dissemination of tst-1-positive clonal complex 5 (cc5) methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa), is one of the most prevalent clinical pathogens isolated from hospital settings, and has increasingly identified in community settings. in china, the sccmeciii-st239 strains are disseminated in different geographic regions, accounting for >75% of all mrsa isolates in some national studies. here we characterized 150 non-duplicate mrsa isolates collected from february 2012 to may 2013 in a tertiary hospital in suzhou, eastern china, to explore th ...201728409124
anti-bacterial and anti-biofilm evaluation of thiazolopyrimidinone derivatives targeting the histidine kinase yycg protein of staphylococcus epidermidis.staphylococcus epidermidis is one of the most important opportunistic pathogens in nosocomial infections. the main pathogenicity associated with s. epidermidis involves the formation of biofilms on implanted medical devices, biofilms dramatically decrease the efficacy of conventional antibiotics and the host immune system. this emphasizes the urgent need for designing novel anti-staphylococcal biofilm agents. based on the findings that compound 5, targeting the histidine kinase domain of s. epid ...201728408903
shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles.gold nanoparticles (aunps) of various shapes (including spheres, stars and flowers), with similar dimensions, were synthesized and evaluated for their antibacterial effects toward staphylococcus aureus, a bacterium responsible for numerous life-threatening infections worldwide. optical growth curve measurements and gompertz modeling showed significant aunp shape- and concentration-dependent decreases in bacterial growth with increases in bacterial growth lag time. to evaluate prospective use in ...201728408817
draft genome sequences of two clinical methicillin-resistant staphylococcus aureus strains isolated from healthy children in brazil.we report here the draft genome sequences of two community-associated methicillin-resistant staphylococcus aureus (ca-mrsa) strains, c18 and c80, isolated from healthy children from day care centers. to our knowledge, these are the first draft genome sequences of ca-mrsa st398/cc398/sccmecv and ca-mrsa st5/cc5/sccmeciva isolated from healthy children in brazil.201728408675
draft genome sequence of methicillin-resistant staphylococcus aureus strain lc33 isolated from human breast milk.here, we report the draft genome sequence of staphylococcus aureus strain lc33, isolated from human breast milk in brazil. this microorganism has been typed as st1/t127/sccmecv. to our knowledge, this is the first draft genome sequence of a methicillin-resistant s. aureus strain isolated from human breast milk.201728408673
pharmacokinetics of linezolid during continuous hemodiafiltration: a case report.the pharmacokinetics of linezolid clearance (cllzd) during continuous hemodiafiltration (chdf) has not been comprehensively analyzed. here, we examined cllzd by chdf in a patient with septic shock and disseminated intravascular coagulation due to methicillin-resistant staphylococcus aureus. the extraction ratio of lzd by chdf was 22.6%, and the protein-binding rate was 17.9% ± 7.7%. in addition, it was determined that the calculated total body clearance of lzd was 30.2 ml/min, plasma elimination ...201728408302
prevalence of methicillin-resistant staphylococcus aureus (mrsa) infection and the molecular characteristics of mrsa bacteraemia over a two-year period in a tertiary teaching hospital in malaysia.methicillin-resistant staphylococcus aureus (mrsa) is an established pathogen that causes hospital- and community-acquired infections worldwide. the prevalence rate of mrsa infections were reported to be the highest in asia. as there is limited epidemiological study being done in malaysia, this study aimed to determine the prevalence of mrsa infection and the molecular characteristics of mrsa bacteraemia.201728407796
erratum to: antibiotic resistance and biofilm production among the strains of staphylococcus aureus isolated in a tertiary care hospital in nepal. 201728407762
the expression of fibronectin is significantly suppressed in macrophages to exert a protective effect against staphylococcus aureus infection.fibronectin (fn) plays a major role in the attachment of staphylococcus aureus to host cells by bridging staphylococcal fibronectin-binding proteins (fnbps) and cell-surface integrins. a previous study demonstrated that the phagocytosis of s. aureus by macrophages is enhanced in the presence of exogenous fn. we recently found that fnbps overexpression also enhances phagocytic activity. the effect of s. aureus infection on the expression of macrophage fn was investigated.201728407745
staphylococcus sciuri bacteriophages double-convert for staphylokinase and phospholipase, mediate interspecies plasmid transduction, and package meca gene.staphylococcus sciuri is a bacterial pathogen associated with infections in animals and humans, and represents a reservoir for the meca gene encoding methicillin-resistance in staphylococci. no s. sciuri siphophages were known. here the identification and characterization of two temperate s. sciuri phages from the siphoviridae family designated ϕ575 and ϕ879 are presented. the phages have icosahedral heads and flexible noncontractile tails that end with a tail spike. the genomes of the phages ar ...201728406168
daptomycin-associated eosinophilic pneumonia with rechallenge: a case report
.a case of daptomycin-associated acute eosinophilic pneumonia (aep) with positive rechallenge is reported.201728406091
comparison of whole-genome sequencing methods for analysis of three methicillin-resistant staphylococcus aureus outbreaks.whole-genome sequencing (wgs) can provide excellent resolution in global and local epidemiological investigations of staphylococcus aureus outbreaks. a variety of sequencing approaches and analytical tools have been used; it is not clear which is ideal. we compared two wgs strategies and two analytical approaches to the standard method of smai restriction digestion pulsed-field gel electrophoresis (pfge) for typing s. aureus forty-two s. aureus isolates from three outbreaks and 12 reference isol ...201728404677
the effects of ozone on bacterial growth and thiol-disulphide homeostasis in vascular graft infection caused by mrsa in rats.to investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated.201728403346
bacterial osteomyelitis or nonbacterial osteitis in children: a study involving the german surveillance unit for rare diseases in childhood.although bacterial osteomyelitis (bo) is a commonly recognized diagnosis in pediatrics, it is often difficult to distinguish from nonbacterial osteitis (nbo). the goal of our study was to distinguish between the 2 disease entities and better define nbo.201728403046
investigation of a staphylococcal food poisoning outbreak from a chantilly cream dessert, in umbria (italy).on august 28, 2015, a staphylococcal food poisoning outbreak occurred in umbria, italy, affecting 24 of the 42 customers who had dinner at a local restaurant. about 3 h after ingesting a variety of foods, the customers manifested gastrointestinal symptoms. within 24 h of notification from the hospital emergency department, sanitary inspectors of the local public health unit performed an epidemiological investigation. a retrospective cohort study was conducted among the customers. food and enviro ...201728402712
tight binding of heme to staphylococcus aureus isdg and isdi precludes design of a competitive inhibitor.the micromolar equilibrium constants for heme dissociation from isdg and isdi reported in the literature call into question whether these enzymes are actually members of the iron-regulated surface determinant system of staphylococcus aureus, which harvests heme iron from a host during infection. in order to address this question, the heme dissociation constants for isdg and isdi were reevaluated using three approaches. the heme dissociation equilibrium constants were measured using a uv/vis abso ...201728401968
vancomycin prophylaxis for total joint arthroplasty: incorrectly dosed and has a higher rate of periprosthetic infection than cefazolin.in total joint arthroplasty (tja), vancomycin is used as perioperative antibiotic prophylaxis in patients with penicillin allergy or in patients colonized with methicillin-resistant staphylococcus aureus (mrsa). although vancomycin dosing should be weight-based (15 mg/kg), not all surgeons are aware of this; a fixed 1-g dose is instead frequently administered.201728401341
metastatic endogenous endophthalmitis: a rare presentation with methicillin-resistant staphylococcus aureus prostatic abscess.a 62-year-old male with the previous history of uncontrolled diabetes and hypertension on irregular treatment presented with a history of fever, dysuria, and urinary retention with progressive painful loss of vision over a period of 2 days. his eye examination showed hypopyon, and he was diagnosed to have rapidly progressive endogenous endophthalmitis. he was started on vancomycin and piperacillin-tazobactam empirically. his blood and urine cultures grew methicillin-resistant staphylococcus aure ...201728400691
amino acid polymorphisms in the fibronectin-binding repeats of fibronectin-binding protein a affect bond strength and fibronectin conformation.the staphylococcus aureus cell surface contains cell wall-anchored proteins such as fibronectin-binding protein a (fnbpa) that bind to host ligands (e.g. fibronectin; fn) present in the extracellular matrix of tissue or coatings on cardiac implants. recent clinical studies have found a correlation between cardiovascular infections caused by s. aureus and nonsynonymous snps in fnbpa. atomic force microscopy (afm), surface plasmon resonance (spr), and molecular simulations were used to investigate ...201728400484
derepression of sapibov1 is independent of φnm1 type 2 dutpase activity and is inhibited by dutp and dump.staphylococcus aureus is an opportunistic human pathogen able to transfer virulence genes to other cells through the mobilization of s. aureus pathogenicity islands (sapis). sapis are derepressed and packaged into phage-like transducing particles by helper phages like 80α or φnm1. phages 80α and φnm1 encode structurally distinct dutpases, dut80α (type 1) and dutnm1 (type 2). both dutpases can interact with the sapibov1 stl master repressor, leading to derepression and mobilization. that two stru ...201728400210
east and west african milk products are reservoirs for human and livestock-associated staphylococcus aureus.staphylococcus aureus frequently isolated from milk products in sub-saharan africa (ssa) is a major pathogen responsible for food intoxication, human and animal diseases. ssa hospital-derived strains are well studied but data on the population structure of foodborne s. aureus required to identify possible staphylococcal food poisoning sources is lacking. therefore, the aim was to assess the population genetic structure, virulence and antibiotic resistance genes associated with milk-derived s. au ...201728400021
effect of sub-inhibitory concentrations of biocides on the architecture and viability of mrsa biofilms.the effect of sub-minimum inhibitory concentrations (sub-mics) of three biocides (benzalkonium chloride [bzk], trisodium phosphate [tsp] and sodium hypochlorite [shy]) upon the architecture and viability of the biofilms formed by a methicillin-resistant staphylococcus aureus strain of food origin (mrsa 48a) was investigated. images were examined through confocal laser scanning microscopy (clsm) after staining with syto9 and propidium iodide. sub-mics of bzk or tsp reduced the ability of mrsa to ...201728400016
effect of food-related stress conditions and loss of agr and sigb on seb promoter activity in s. aureus.staphylococcal enterotoxin b (seb) causes staphylococcal food poisoning and is produced in up to ten times higher quantities than other major enterotoxins. while staphylococcus aureus growth is often repressed by competing flora, the organism exhibits a decisive growth advantage under some stress conditions. so far, data on the influence of food-related stressors and regulatory mutations on seb expression is limited and largely based on laboratory strains, which were later reported to harbor mut ...201728400004
prevalence and molecular characterization of staphylococcus aureus in commercially available meat over a one-year period in iowa, usa.methicillin-resistant staphylococcus aureus (mrsa) is a leading cause of infectious disease morbidity and mortality. previous studies have confirmed the presence of s. aureus, including mrsa, on raw meat products. we investigated the prevalence and molecular epidemiology of s. aureus and mrsa in commercially-distributed antibiotic-free and conventional raw meat products (n = 3290) purchased in 8 iowa retail stores weekly for a period of one year. isolates were characterized using spa typing, and ...201728399994
factors associated with staphylococcus aureus nasal carriage and molecular characteristics among the general population at a medical college campus in guangzhou, south china.the nasal cavity is the main colonization site of staphylococcus aureus (s. aureus) in human body. nasal carriage may be a strong risk factor for some serious infection. there was still limited information about the nasal carriage for s. aureus in south china.201728399856
frequent isolation of methicillin resistant staphylococcus aureus (mrsa) st398 among healthy pigs in portugal.although livestock-associated st398 methicillin-resistant staphylococcus aureus (mrsa) has been widely reported in different geographic regions, mrsa carriage studies among healthy pigs in portugal are very limited.201728399155
the recent emergence in hospitals of multidrug-resistant community-associated sequence type 1 and spa type t127 methicillin-resistant staphylococcus aureus investigated by whole-genome sequencing: implications for screening.community-associated spa type t127/t922 methicillin-resistant staphylococcus aureus (mrsa) prevalence increased from 1%-7% in ireland between 2010-2015. this study tracked the spread of 89 such isolates from june 2013-june 2016. these included 78 healthcare-associated and 11 community associated-mrsa isolates from a prolonged hospital outbreak (h1) (n = 46), 16 other hospitals (n = 28), four other healthcare facilities (n = 4) and community-associated sources (n = 11). isolates underwent antimic ...201728399151
staphylococcus aureus central nervous system infections in children.central nervous system (cns) infections due to staphylococcus aureus are uncommon in pediatric patients. we review the epidemiology, clinical features, and treatment in 68 patients with a s. aureus cns infection evaluated at texas children's hospital (tch).201728399057
potential involvement of the epidermal growth factor receptor ligand epiregulin and matrix metalloproteinase-1 in pathogenesis of chronic rhinosinusitis.chronic rhinosinusitis (crs) is a heterogeneous chronic inflammatory disease of the nose and paranasal sinuses that presents without (crssnp) or with nasal polyps (crswnp). features of crswnp are the frequent presence of type 2 allergic inflammation and high prevalence of staphylococcus aureus (sa) colonization. as inflammation persists, sinus tissue undergoes epithelial damage and repair along with polyp growth. since one feature of damaged tissue is enhancement of growth factor signaling, we e ...201728398769
prognosis associated with redo cardiac resynchronization therapy following complete device and lead extraction due to device-related infection.an increase in the number of cardiac resynchronization therapy (crt) device implantations worldwide has led to a consequent increase in the number of infections associated with the device, making extraction of the crt device inevitable. redo crt implantation after treatment and recovery following device extraction is challenging. this study aimed to evaluate the success rate, complications, and long-term prognosis of redo crt implantation, including the rates of subclavian, cava, and coronary si ...201728398556
antibiotic-loaded mos<sub>2</sub> nanosheets to combat bacterial resistance via biofilms inhibition.the emergence of antibiotic resistance has resulted in an increasing difficulty treating clinical infections associated with biofilms formation, one of the key processes contributed to enhance antibiotic resistance in return. with the rapid development of nanotechnology, a new way to overcome antibiotic resistance was opened up. based on multiple properties especially antibacterial potential of mos<sub>2</sub> nanosheets that have aroused wide attention, herein, a novel antimicrobial agent to co ...201728398220
ph-dependent antimicrobial properties of copper oxide nanoparticles in staphylococcus aureus.the antimicrobial properties of cuo nanoparticles have been investigated, but the underlying mechanisms of toxicity remain the subject of debate. here, we show that cuo nanoparticles exhibit significant toxicity at ph 5 against four different staphylococcus aureus (s. aureus) strains, including newman, sa113, usa300, and atcc6538. at this ph, but not at ph 6 and 7, 5 mm cuo nanoparticles effectively caused reduction of sa113 and newman cells and caused at least 2 log reduction, whereas 20 mm kil ...201728397766
the incidence of mrsa infections in the united states: is a more comprehensive tracking system needed?a review of epidemiological studies on the incidence of mrsa infections overtime was performed along with an analysis of data available for download from hospital compare (https://data.medicare.gov/data/hospital-compare). we found the estimations of the incidence of mrsa infections varied widely depending upon the type of population studied, the types of infections captured and in the definitions and terminology used to describe the results. we could not find definitive evidence that the inciden ...201728396730
methicillin-resistant staphylococcus aureus and antibiotic use in septorhinoplasty: case report and review of literature.septorhinoplasty is a commonly performed procedure for facial aesthetics and obstructed nasal breathing. there have been only 4 reported cases of methicillin-resistant staphylococcus aureus (mrsa)-associated postoperative complications following septorhinoplasty reported in the literature across all specialties. in this article, we report a case of mrsa-associated infection after an uncomplicated septorhinoplasty. risk stratification and outcome of treatment are described, followed by a review o ...201728396072
vancomycin-intermediate staphylococcus aureus isolates are attenuated for virulence when compared with susceptible progenitors.vancomycin-intermediate staphylococcus aureus (visa) is associated with genetic changes that may also impact upon pathogenicity. in the current study, we compared the virulence of clinical visa strains with their isogenic vancomycin-susceptible progenitors (vssa).201728396035
[efficacy and safety of linezolid among patients with methicillin-resistant staphylococcus aureus bacteremia].objective: to study the efficacy and safety of linezolid for the treatment of patients with bacteremia caused by methicillin-resistant staphylococcus aureus (mrsa). methods: totally 52 cases of mrsa bacteremia patients, from january 2010 to april 2014 in the first affiliated hospital, school of medicine, zhejiang university, were retrospectively analyzed. they were classified into two groups based on linezolid therapeutic regimen: primary treatment with linezolid (19 cases) and alternated to lin ...201728395434
how are the ancient cystic fibrosis patients? cystic fibrosis diagnosed over 60 years-old.to specify the prevalence of patients diagnosed with cf at age of ≥60 year-old and to analyze their characteristics.201728393935
anti-inflammatory activity of crateva adansonii dc on keratinocytes infected by staphylococcus aureus: from traditional practice to scientific approach using hptlc-densitometry.leaves of crateva adansonii dc (capparidaceae), a small bush found in togo, are widely used in traditional medicine to cure infectious abscesses. traditional healers of lomé harvest only budding leaves early in the morning, in specific area in order to prepare their drugs.201728392456
ester- and amide-containing multiqacs: exploring multicationic soft antimicrobial agents.quaternary ammonium compounds (qacs) are ubiquitous antiseptics whose chemical stability is both an aid to prolonged antibacterial activity and a liability to the environment. soft antimicrobials, such as qacs designed to decompose in relatively short times, show the promise to kill bacteria effectively but not leave a lasting footprint. we have designed and prepared 40 soft qac compounds based on both ester and amide linkages, in a systematic study of mono-, bis-, and tris-cationic qac species. ...201728392192
design and development of some thiazole-based flavanoids as novel antibacterial against pathogens causing surgical site infection for possible benefit in bone trauma via inhibition of dna gyrase.in this study, a novel class of hybrid thiazole-based flavanoid derivatives were synthesized and characterized by ft-ir, (1) h-nmr, (13) c-nmr, mass and elemental analysis. these derivatives were evaluated for antibacterial activity for possible benefit in bone trauma via inhibition of dna gyrase enzyme. results suggested that compounds 9n, 9o, and 9p showed considerable inhibition of dna gyrase with considerable activity against tested forty strains of staphylococcus aureus clinical isolates. m ...201728388011
biological characteristics of staphylococcal enterotoxin q and its potential risk for food poisoning.to elucidate the biological characteristics and stability of a newly identified staphylococcal enterotoxin q (seq) against heating and digestive enzymes and to evaluate the risk of seq-harbouring staphylococcus aureus in food poisoning.201728375567
ankylosis of the knee after septic arthritis following anterior cruciate ligament reconstruction in a 13-year-old male.we present the case of an adolescent male developing an ankylosis of the knee after septic arthritis following anterior cruciate ligament reconstruction (aclr). the patient was shifted to our institution with postoperative septic arthritis associated with a systemic septic condition. before, repeated arthroscopic surgery had been conducted without any improvement. mri showed a concomitant osteomyelitis. the infection (gächter iv, staphylococcus aureus) was controlled by an open surgical approach ...201728342819
international experts' practice in the antibiotic therapy of infective endocarditis is not following the guidelines.the management of infective endocarditis (ie) may differ from international guidelines, even in reference centres. this is probably because most recommendations are not based on hard evidence, so the consensus obtained for the guidelines does not represent actual practices. for this reason, we aimed to evaluate this question in the particular field of antibiotic therapy.201728323194
the effect of negative pressure wound therapy with antiseptic instillation on biofilm formation in a porcine model of infected spinal instrumentation.this study evaluates the effect of negative pressure wound therapy with antiseptic instillation (npwti) in the clearance of infection and biofilm formation in an in vivo model of infected spinal implants compared to traditional treatment modalities.201728267679
comparing the cost-effectiveness of linezolid to trimethoprim/sulfamethoxazole plus rifampicin for the treatment of methicillin-resistant staphylococcus aureus infection: a healthcare system perspective.few industry-independent studies have been conducted to compare the relative costs and benefits of drugs to treat methicillin-resistant staphylococcus aureus (mrsa) infection. we performed a stochastic cost-effectiveness analysis comparing two treatment strategies-linezolid versus trimethoprim-sulfamethoxazole plus rifampicin-for the treatment of mrsa infection.201728232163
prevention of staphylococcus aureus ventilator-associated pneumonia: conventional antibiotics won't cut it. 201728158521
staphylococcus aureus in the intensive care unit: are these golden grapes ripe for a new approach?staphylococcus aureus is the leading cause of infection in the setting of critical illness and injury. this pathogen causes life-threatening infection in otherwise healthy individuals and also complicates the clinical course of patients requiring intensive care as a result of their primary medical or surgical disease processes. s. aureus infection in the intensive care unit (icu) most commonly manifests as sepsis, ventilator-associated pneumonia, and infection of surgical sites and indwelling me ...201728003353
prevalence of vancomycin resistant staphylococcus aureus (vrsa) in methicillin resistant s. aureus (mrsa) strains isolated from burn wound infections.the increase in resistance of methicillin resistant staphylococcus aureus (mrsa) strains to vancomycin has been perceived as a formidable threat in the therapeutic fields. the present study investigated the vancomycin resistance traits of mrsa isolates [vancomycin resistant s. aureus (vrsa)] collected from burn patients.201728757721
identification of a distinctive phenotype for endocarditis-associated clonal complex 22 mrsa isolates with reduced vancomycin susceptibility.we previously identified an association between cc22 meticillin-resistant staphylococcus aureus (mrsa) bloodstream infection isolates with an elevated vancomycin mic (v-mic) in the susceptible range (1.5-2 mg l-1) and endocarditis. this study explores whether these isolates have a specific phenotype consistent with the clinical findings.201728504620
[pediatric case report: spinal epidural abscess].spinal epidural abscess is an uncommon pathology. it has an incidence of one case per 100 000 individuals. an increase is observed due to the raise of risk factors such as diabetes mellitus, spinal abnormalities, tattoos, acupuncture, epidural analgesia, and a greater availability of imaging methods. it is a purulent collection located between the dura and the medullary canal. the most common germs are staphylococcus aureus and gram-negative bacteria. without timely treatment, it evolves to medu ...201728504498
staphylococcus aureus nasal carriage and its antibiotic resistance profiles in children in high altitude areas of southwestern china.to describe the epidemiological profile of nasal carriage of staphylococcus aureus (s. aureus) strains, its antibiotic resistance and meca and panton valentine leukocidin (pvl) genes presence, in school children residing in high altitude areas of southwestern china.201728504494
genetic characterization of staphylococcus aureus isolated from nasal samples of healthy ewes in tunisia. high prevalence of cc130 and cc522 lineages.staphylococcus aureus is a versatile bacterium, which can infect or colonize a variety of host species. the objective of this study was to characterize s. aureus isolates recovered from nasal swabs of 167 healthy ewes sampled from 12 farms in different areas of tunisia during the period of 2014-2015. genetic lineages, virulence factors and antibiotic resistance mechanisms were determined for recovered isolates. s. aureus was detected in 45 out of 167 tested samples (26.9%). all isolates were met ...201728504093
combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of staphylococcus aureus.a staphylococcus aureus (s. aureus)-specific lytic bacteriophage p-s. aureus-9, isolated from an environmental water sample, was assembled on magnetic beads for capturing s. aureus from samples through magnetic separation. horseradish peroxidase (hrp) labeled immunoglobulin (igg) antibodies were used to detect the captured s. aureus by reacting with protein a on s. aureus followed by colorimetric signals, which were generated from the catalytic reaction between hrp and the substrate 3,3',5,5'-te ...201728501172
association between staphylococcus aureus bacteremia and hospital mortality in hemodialysis patients with bloodstream infection: a multicenter cohort from japanese tertiary care centers.multiple studies have shown that staphylococcus aureus bacteremia (sab) has been a major cause of death in hemodialysis patients. we examined whether sab is a risk for mortality among chronic hemodialysis patients in japan where the standard vascular access is arteriovenous fistula (avf). this was a multicenter, retrospective study of maintenance hemodialysis patients with bloodstream infection (bsi) from 2011 to 2013 at tertiary care centers in japan. the endpoint was hospital mortality. our co ...201728498647
infective endocarditis; report from a main referral teaching hospital in iran.the aim of the present preliminary study was to assess the demographic, clinical, paraclinical, microbiological, echocardiographic, and therapeutic profile as well as in-hospital outcome of patients with infective endocarditis at a referral center for various infectious diseases in iran.201728496492
multifunctional and biomimetic fish collagen/bioactive glass nanofibers: fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo.the development of skin wound dressings with excellent properties has always been an important challenge in the field of biomedicine. in this study, biomimetic electrospun fish collagen/bioactive glass (col/bg) nanofibers were prepared. their structure, tensile strength, antibacterial activity and biological effects on human keratinocytes, human dermal fibroblasts and human vascular endothelial cells were investigated. furthermore, the sprague dawley rat skin defect model was used to validate th ...201728496325
complete genome sequence of the livestock-associated methicillin-resistant strain staphylococcus aureus subsp. aureus 08s00974 (sequence type 398).we report here the complete genome sequence of the livestock-associated methicillin-resistant staphylococcus aureus strain 08s00974 from sequence type 398 (st398 la-mrsa) isolated from a fatting pig at a farm in germany.201728495772
complete genome sequence of the disinfectant susceptibility testing reference strain staphylococcus aureus subsp. aureus atcc 6538.we report here the complete genome sequence of the methicillin-sensitive staphylococcus aureus subsp. aureus strain atcc 6538 (fda 209, dsm 799, wdcm 00032, and nctc 10788).201728495771
methicillin-resistant staphylococcus aureus screening pcr adapted to locally emerging variants-evaluation of novel sccmec primers.infections with multi-resistant bacteria, such as methicillin-resistant staphylococcus aureus (mrsa), represent a world-wide health-care problem. the original mrsa screening taqman pcr was based on the detection of the sccmec-orfx-junction as described by the group of huletsky in 2004. in the recent years, this assay increasingly failed to detect new mrsa variants in swab specimens. in this work, we analyzed the usefulness of 17 additional sccmec primers to increase pcr sensitivity by testing 29 ...201728495466
no evidence of genome editing activity from natronobacterium gregoryi argonaute (ngago) in human cells.the argonaute protein from the thermophilic bacterium thermus thermophilus shows dna-guided dna interfering activity at high temperatures, complicating its application in mammalian cells. a recent work reported that the argonaute protein from natronobacterium gregoryi (ngago) had dna-guided genome editing activity in mammalian cells. we compared the genome editing activities of ngago and staphylococcus aureus cas9 (sacas9) in human hek293t cells side by side. egfp reporter assays and dna sequenc ...201728494027
fluorescent pcr detection of meca in drug resistant mrsa: a methodological study. 201728493763
new azulene-type sesquiterpenoids from the fruiting bodies of lactarius deliciosus.in the (1)h nmr-guided fractionation of extracts from the edible mushroom lactarius deliciosus, two new azulene-type sesquiterpenoids, 7-isopropenyl-4-methyl-azulene-1-carboxylic acid (1) and 15-hydroxy-3,6-dihydrolactarazulene (2), together with seven known compounds were characterized. their structures were determined on basis of spectroscopic evidence, as well as by comparing with literature data. amongst the known metabolites, the (13)c nmr assignment of 15-hydroxy-6,7-dihydrolactarazulene ( ...201728493207
[management of multiresistant bacteria in urology].contamination and infection with extensive drug resistant (xdr) bacteria are increasing in urology with the exception of methicillin resistant staphylococcus aureus (mrsa) (stabilization). they often lead to logistic and therapeutical problems. only 30-50% of xdr cases are of exogenous origin. to slow this trend, screening, hygiene programs, isolation, decontamination, targeted therapy of symptomatic infections, education programs, and success controls should be applied. furthermore, all regulat ...201728493114
impedimetric measurement of dna-dna hybridisation using microelectrodes with different radii for detection of methicillin resistant staphylococcus aureus (mrsa).due to their electroanalytical advantages, microelectrodes are a very attractive technology for sensing and monitoring applications. one highly important application is measurement of dna hybridisation to detect a wide range of clinically important phenomena, including single nucleotide polymorphisms (snps), mutations and drug resistance genes. the use of electrochemical impedance spectroscopy (eis) for measurement of dna hybridisation is well established for large electrodes but as yet remains ...201728492640
neonatal sepsis in rural india: timing, microbiology and antibiotic resistance in a population-based prospective study in the community setting.to examine the timing and microbiology of neonatal sepsis in a population-based surveillance in the indian community setting.201728492525
septic shock due to implantable cardiac defibrillator related infection.infection is an important complication of cardiac device implantation. we report the case of a 61 year old patient presenting with septic shock caused by cardiac device infection (cdi) three-weeks after device implantation. at initial presentation, there was an absence of both localising signs and echocardiographic evidence of cdi. later, staphylococcus aureus was cultured from blood and the pre-pectoral pocket. 48 hours after admission the device and leads were explanted in theatre by simple tr ...201728496687
simultaneous antibiofilm and antiviral activities of an engineered antimicrobial peptide during virus-bacterium coinfection.antimicrobial-resistant infections are an urgent public health threat, and development of novel antimicrobial therapies has been painstakingly slow. polymicrobial infections are increasingly recognized as a significant source of severe disease and also contribute to reduced susceptibility to antimicrobials. chronic infections also are characterized by their ability to resist clearance, which is commonly linked to the development of biofilms that are notorious for antimicrobial resistance. the us ...201727303744
effect of human burn wound exudate on pseudomonas aeruginosa virulence.burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. infections by notorious pathogens such as pseudomonas aeruginosa, staphylococcus aureus, and acinetobacter baumannii impair patient recovery and can even lead to fatality. in this study, we investigated the effect of burn wound exudates (bwes) on the virulence of those pathogens. bwes were collected within 7 days after burn trauma from 5 burn patients. we first monitored their effect on pathogen growth. i ...201727303724
cathodic voltage-controlled electrical stimulation of titanium for prevention of methicillin-resistant staphylococcus aureus and acinetobacter baumannii biofilm infections.antibiotic resistance of bacterial biofilms limits available treatment methods for implant-associated orthopaedic infections. this study evaluated the effects of applying cathodic voltage-controlled electrical stimulations (cvces) of -1.5v and -1.8v (vs. ag/agcl) to coupons of commercially pure titanium (cpti) incubated in cultures of methicillin-resistant staphylococcus aureus (mrsa) and acinetobacter baumannii (a. baumannii) as a method of preventing bacterial attachment. stimulations were app ...201727890730
detection of eskape bacterial pathogens at the point of care using isothermal dna-based assays in a portable degas-actuated microfluidic diagnostic assay platform.an estimated 1.5 billion microbial infections occur globally each year and result in ∼4.6 million deaths. a technology gap associated with commercially available diagnostic tests in remote and underdeveloped regions prevents timely pathogen identification for effective antibiotic chemotherapies for infected patients. the result is a trial-and-error approach that is limited in effectiveness, increases risk for patients while contributing to antimicrobial drug resistance, and reduces the lifetime ...201727986722
refugees and antimicrobial resistance: a systematic review.there is a large increase in the numbers of refugees and asylum seekers worldwide and a lack of data on the carriage of antimicrobial resistance in refugee/asylum seeking groups.201727919742
Refresh
firstprev
...1611161216131614161516161617161816191620...
nextlast
Displaying items 161501 - 161600 of 163583