Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| association between the agr locus and the presence of virulence genes and pathogenesis in staphylococcus aureus using a caenorhabditis elegans model. | staphylococcus aureus is a commensal pathogen with a virulon that is under agr control. agr dysfunction has been seen in clinical strains that do not respond positively to treatment. this study aimed to establish the association between the genes in the virulon and the presence of agr and to determine the relationship between the presence or absence of agr and pathogenicity. | 2017 | 27915107 |
| design and surface immobilization of short anti-biofilm peptides. | short antimicrobial peptides are essential to keep us healthy and their lasting potency can inspire the design of new types of antibiotics. this study reports the design of a family of eight-residue tryptophan-rich peptides (tetraf2w) obtained by converting the four phenylalanines in temporin-shf to tryptophans. the temporin-shf template was identified from the antimicrobial peptide database (http://aps.unmc.edu/ap). remarkably, the double arginine variant (tetraf2w-rr) was more effective in kil ... | 2017 | 27915018 |
| expanding the clinical and genetic spectrum of g6pd deficiency: the occurrence of bcgitis and novel missense mutation. | glucose-6-phosphate dehydrogenase (g6pd) is a key enzyme in the pentose phosphate pathway that ensures sufficient production of coenzyme nicotinamide adenine dinucleotide phosphate (nadph) by catalyzing the reduction of nadp+ to nadph. noteworthy, the latter mediates the production of reactive oxygen species (ros) by phagocytic cells such as neutrophils and monocytes. therefore, patients with severe forms of g6pd deficiency may present impaired nadph oxidase activity and become susceptible to re ... | 2017 | 27914961 |
| immobilizing bacitracin on titanium for prophylaxis of infections and for improving osteoinductivity: an in vivo study. | bacitracin immobilized on the titanium (ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro. in the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified ti in vivo. a rat osteomyelitis model with femoral medullary cavity placement of ti rods was employed to analyze the prophylactic effect of bacitracin-modified ti (ti-bc). thirty-six female sprague dawley (sd) rats were used to estab ... | 2017 | 27914255 |
| impact of initial vancomycin trough concentration on clinical and microbiological outcomes of methicillin-resistant staphylococcus aureus bacteremia in children. | it is important to use vancomycin in a proper manner to ensure optimal drug exposure. despite extensive use of vancomycin in children, studies on its optimal trough concentration (ctrough) in the pediatric population remained rare. this retrospective study included children < 18 years old with culture-confirmed methicillin-resistant staphylococcus aureus (mrsa) bacteremia who were hospitalized in our institute from january 2010 to april 2014. clinical characteristics, initial vancomycin dose, ct ... | 2017 | 27914127 |
| osprey predicts resistance mutations using positive and negative computational protein design. | drug resistance in protein targets is an increasingly common phenomenon that reduces the efficacy of both existing and new antibiotics. however, knowledge of future resistance mutations during pre-clinical phases of drug development would enable the design of novel antibiotics that are robust against not only known resistant mutants, but also against those that have not yet been clinically observed. computational structure-based protein design (cspd) is a transformative field that enables the pr ... | 2017 | 27914058 |
| surgical site infections in major head and neck surgeries involving pedicled flap reconstruction. | to evaluate surgical site infections (ssi) after pedicled reconstruction in head and neck surgery. | 2017 | 27913719 |
| identification of microorganisms on mobile phones of intensive care unit health care workers and medical students in the tertiary hospital. | aim to identify and investigate a difference between microorganisms present on intensive care unit (icu) health care workers' (hcw, doctors, nurses or medical technicians) and medical students' mobile phones as well as to investigate a difference between the frequency and the way of cleaning mobile phones. methods fifty swabs were collected from hcws who work in the icu (university hospital centre osijek) and 60 swabs from medical students (school of medicine, university of osijek). microorganis ... | 2017 | 27917855 |
| staphylococcus lugdunensis endocarditis with destruction of the ventricular septum and multiple native valves. | staphylococcus lugdunensis (s. lugdunensis) is a coagulase negative staphylococcus (cons) that can cause destructive infective endocarditis. s. lugdunensis, unlike other cons, should be considered to be a pathogen. we report the first case of s. lugdunensis endocarditis causing ventricular septal defect and destruction of the aortic and mitral valves. a 53-year-old male with morbid obesity and copd presented with intermittent fever and progressive shortness of breath for 2 weeks. chest examinati ... | 2017 | 27920984 |
| intracellular staphylococcus aureus control by virulent bacteriophages within mac-t bovine mammary epithelial cells. | bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. the present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular staphylococcus aureus in a time-dependent manner. in particular, phage vb_saum_js25 reached the nucleus within 3 h postincubation. the phages had an endocytotic efficiency of 12%. this ability to kill intracellular host bacteria suggests the utility of phage-based therapies and may protect patient ... | 2017 | 27919889 |
| phenylthiazole antibiotics: a metabolism-guided approach to overcome short duration of action. | antibacterial resistance is a pressing global health challenge that necessitates the development of new therapeutic agents. phenylthiazole antibacterial agents have been extensively studied, by our group, as a potential novel class of antibiotics to circumvent the scourge of antibacterial resistance. previously, the phenylthiazole lead compound 1 was shown to possess potent activity against clinical isolates of methicillin- and vancomycin-resistant staphylococcus aureus (mrsa and vrsa). the prom ... | 2017 | 27918995 |
| clinical utility of methicillin-resistant staphylococcus aureus nasal polymerase chain reaction assay in critically ill patients with nosocomial pneumonia. | this study investigated the diagnostic performance characteristics of a methicillin-resistant staphylococcus aureus (mrsa) nasal polymerase chain reaction (pcr) assay in critically ill patients with nosocomial pneumonia. | 2017 | 27918901 |
| vaccine against toxic shock syndrome in a first-in-man clinical trial. | 2017 | 27918218 | |
| cholinergic pet imaging in infections and inflammation using (11)c-donepezil and (18)f-feobv. | immune cells utilize acetylcholine as a paracrine-signaling molecule. many white blood cells express components of the cholinergic signaling pathway, and these are up-regulated when immune cells are activated. however, in vivo molecular imaging of cholinergic signaling in the context of inflammation has not previously been investigated. | 2017 | 27785538 |
| pharmacokinetics and pharmacodynamics of dsta4637a: a novel thiomab™ antibody antibiotic conjugate against staphylococcus aureus in mice. | dsta4637a, a novel thiomab™ antibody antibiotic conjugate (tac) against staphylococcus aureus (s. aureus), is currently being investigated as a potential therapy against s. aureus infections. structurally, tac is composed of an anti-s. aureus antibody linked to a potent antibiotic, dmdna31. the goal of the current study was to characterize the pharmacokinetics (pk) of tac in mice, assess the effect of s. aureus infection on its pk, and evaluate its pharmacodynamics (pd) by measuring the bacteria ... | 2017 | 27653831 |
| signs of stigma and poor mental health among carriers of mrsa. | many countries have implemented guidelines to prevent transmission of meticillin-resistant staphylococcus aureus (mrsa). important contextual factors of stigma can be identified in the context of mrsa. over the past decade, concerns have been raised over a possible stigmatizing effect of these actions. | 2017 | 27789041 |
| differences in prevalence of community-associated mrsa and mssa among u.s. and non-u.s. born populations in six new york community health centers. | staphylococcus aureus is the most common cause of skin and soft tissue infections (sstis) in the community in the united states of america. community health centers (chc) serve as primary care providers for thousands of immigrants in new york. | 2017 | 27773780 |
| hypoxic regulation of neutrophil function and consequences for staphylococcus aureus infection. | staphylococcal infection and neutrophilic inflammation can act in concert to establish a profoundly hypoxic environment. in this review we summarise how neutrophils and staphylococcus aureus are adapted to function under hypoxic conditions, with a particular focus on the impaired ability of hypoxic neutrophils to effect staphylococcus aureus killing. | 2017 | 27789256 |
| application of doe approach in the development of mini-capsules, based on biopolymers and manuka honey polar fraction, as powder formulation for the treatment of skin ulcers. | the aim of the present work was the development of a powder formulation for the delivery of manuka honey (mh) bioactive components in the treatment of chronic skin ulcers. in particular pectin (pec)/chitosan glutamate (cs)/hyaluronic acid (ha) mini-capsules were obtained by inverse ionotropic gelation in presence of calcium chloride and subsequently freeze-dried. optimization of unloaded (blank) formulation was performed using doe approach. in a screening phase, the following three factors were ... | 2017 | 27789370 |
| epidemiology and microbiological characterization of clinical isolates of staphylococcus aureus in a single healthcare region of the uk, 2015. | we investigated the epidemiology and characterization of isolates of staphylococcus aureus within the yorkshire and humber (yh) region in the uk. in july 2015, each laboratory within yh (n = 14) was assigned two consecutive days during which all clinical isolates of s. aureus were collected. isolates were tested for antibiotic susceptibilities and the presence of genes encoding methicillin resistance (meca and mecc), panton-valentine leukocidin (pvl) (luks-pv), and efflux-mediated chlorhexidine ... | 2017 | 27790966 |
| turkey meat as source of cc9/cc398 methicillin-resistant staphylococcus aureus in humans? | 2017 | 27794020 | |
| longitudinal investigation of nasopharyngeal methicillin-resistant staphylococcus aureus colonization in early infancy: the patch birth cohort study. | the study aimed to determine the long-term staphylococcus aureus colonization patterns and strain relatedness, and the association between maternal and infant colonization in infancy. | 2017 | 27793735 |
| antibacterial profiling of abietane-type diterpenoids. | abietic and dehydroabietic acid are interesting diterpenes with a highly diverse repertoire of associated bioactivities. they have, among others, shown antibacterial and antifungal activity, potentially valuable in the struggle against the increasing antimicrobial resistance and imminent antibiotic shortage. in this paper, we describe the synthesis of a set of 9 abietic and dehydroabietic acid derivatives containing amino acid side chains and their in vitro antimicrobial profiling against a pane ... | 2017 | 27793449 |
| structure and dynamics study of translation initiation factor 1 from staphylococcus aureus suggests its rna binding mode. | translation initiation, the rate-limiting step in the protein synthesis, is tightly regulated. as one of the translation initiation factors, translation initiation factor 1 (if1) plays crucial roles not only in translation but also in many cellular processes that are important for genomic stability, such as the activity of rna chaperones. here, we characterize the rna interactions and dynamics of if1 from staphylococcus aureus mu50 (if1sa) by nmr spectroscopy. first, the nmr-derived solution str ... | 2017 | 27784646 |
| molecular bases determining daptomycin resistance-mediated resensitization to β-lactams (seesaw effect) in methicillin-resistant staphylococcus aureus. | antimicrobial resistance is recognized as one of the principal threats to public health worldwide, yet the problem is increasing. infections caused by methicillin-resistant staphylococcus aureus (mrsa) strains are among the most difficult to treat in clinical settings due to the resistance of mrsa to nearly all available antibiotics. the cyclic anionic lipopeptide antibiotic daptomycin (dap) is the clinical mainstay of anti-mrsa therapy. the decreased susceptibility to dap (dap resistance [dap(r ... | 2017 | 27795377 |
| safety, tolerability, and pharmacokinetics of medi4893, an investigational, extended-half-life, anti-staphylococcus aureus alpha-toxin human monoclonal antibody, in healthy adults. | medi4893 is an investigational immunoglobulin g1(κ) monoclonal antibody that specifically binds to and neutralizes alpha-toxin, a key staphylococcus aureus virulence factor. a triple-amino-acid substitution, m252y/s254t/t256e, was engineered into the medi4893 fc region to extend its serum half-life. a phase 1, double-blind, dose escalation study was designed to evaluate the safety, tolerability, pharmacokinetics, anti-alpha-toxin-neutralizing activity, and antidrug antibody (ada) response of med ... | 2017 | 27795368 |
| essd, a nuclease effector of the staphylococcus aureus ess pathway. | specialized secretion systems of bacteria evolved for selective advantage, either killing microbial competitors or implementing effector functions during parasitism. earlier work characterized the esat-6 secretion system (ess) of staphylococcus aureus and demonstrated its contribution to persistent staphylococcal infection of vertebrate hosts. here, we identify a novel secreted effector of the ess pathway, essd, that functions as a nuclease and cleaves dna but not rna. essi, a protein of the duf ... | 2017 | 27795323 |
| esse promotes staphylococcus aureus ess-dependent protein secretion to modify host immune responses during infection. | staphylococcus aureus, an invasive pathogen of humans and animals, requires a specialized ess pathway to secrete proteins (esxa, esxb, esxc, and esxd) during infection. expression of ess genes is required for s. aureus establishment of persistent abscess lesions following bloodstream infection; however, the mechanisms whereby effectors of the ess pathway implement their virulence strategies were heretofore not known. here, we show that esse forms a complex with other members of the ess secretion ... | 2017 | 27795322 |
| an intracellular peptidyl-prolyl cis/trans isomerase is required for folding and activity of the staphylococcus aureus secreted virulence factor nuclease. | staphylococcus aureus is an important human pathogen that relies on a large repertoire of secreted and cell wall-associated proteins for pathogenesis. consequently, the ability of the organism to cause disease is absolutely dependent on its ability to synthesize and successfully secrete these proteins. in this study, we investigate the role of peptidyl-prolyl cis/trans isomerases (ppiases) on the activity of the s. aureus secreted virulence factor nuclease (nuc). we identify a staphylococcal cyc ... | 2017 | 27795319 |
| etest® versus broth microdilution for ceftaroline mic determination with staphylococcus aureus: results from premium, a european multicentre study. | to compare the concordance of ceftaroline mic values by reference broth microdilution (bmd) and etest (biomérieux, france) for mssa and mrsa isolates obtained from premium (d372sl00001), a european multicentre study. | 2017 | 27798220 |
| mrsa infections among patients in the emergency department: a european multicentre study. | mrsa is a therapeutic concern worldwide, and a major agent of community-acquired skin and soft tissue infections (ca-sstis). while the us epidemiology of mrsa in ca-sstis is well described and reports the high prevalence of the usa300 clone, data on the european situation are lacking. | 2017 | 27798212 |
| simple synthesis of endophenazine g and other phenazines and their evaluation as anti-methicillin-resistant staphylococcus aureus agents. | community-associated methicillin resistant staphylococcus aureus (ca-mrsa) has become a severe health concern because of its treatment difficulties. herein, we report the synthesis and biological evaluation of two phenazine natural products and a series of phenazines that show promising activities against mrsa with mic values in the low micromolar range. basic studies revealed that these compounds are bacteriostatic agents. the most active compound also displayed promising ic50 values against ha ... | 2017 | 27721155 |
| covalent immobilization of enoxacin onto titanium implant surfaces for inhibiting multiple bacterial species infection and in vivo methicillin-resistant staphylococcus aureus infection prophylaxis. | infection is one of the most important causes of titanium implant failure in vivo a developing prophylactic method involves the immobilization of antibiotics, especially vancomycin, onto the surface of the titanium implant. however, these methods have a limited effect in curbing multiple bacterial infections due to antibiotic specificity. in the current study, enoxacin was covalently bound to an amine-functionalized ti surface by use of a polyethylene glycol (peg) spacer, and the bactericidal ef ... | 2017 | 27799220 |
| time interval between infective endocarditis first symptoms and diagnosis: relationship to infective endocarditis characteristics, microorganisms and prognosis. | to analyze the characteristics and outcome of infective endocarditis (ie) according to the time interval between ie first symptoms and diagnosis. | 2017 | 27607562 |
| differences in humoral immune response between patients with or without nasal carriage of staphylococcus aureus. | the humoral immune response against 43 staphylococcal antigens was compared among hospitalized patients where none of them had any staphylococcal infection on the day of admission with or without nasal staphylococcus aureus carriage. fifty-nine carriers and 59 matched non-carriers were studied. the carriers harbored s. aureus of 35 different spa types, including three t037/st239 methicillin-resistant s. aureus (mrsa) (5.1%). among the 118 patients, 31 acquired s. aureus during hospitalization. i ... | 2017 | 27815779 |
| quantitation of wall teichoic acid in staphylococcus aureus by direct measurement of monomeric units using lc-ms/ms. | the emergence of methicillin-resistant staphylococcus aureus (mrsa) has created an urgent need for new therapeutic agents capable of combating this threat. we have previously reported on the discovery of novel inhibitors targeting enzymes involved in the biosynthesis of wall teichoic acid (wta) and demonstrated that these agents can restore β-lactam efficacy against mrsa. in those previous reports pathway engagement of inhibitors was demonstrated by reduction in wta levels measured by polyacryla ... | 2017 | 27815077 |
| adhesion force of staphylococcus aureus on various biomaterial surfaces. | staphylococcus comprises of more than half of all pathogens in orthopedic implant infections and they can cause major bone infection which can result in destruction of joint and bone. in the current study, adhesion force of bacteria on the surface of various biomaterial surfaces is measured using atomic force microscope (afm). staphylococcus aureus was immobilized on an afm tipless cantilever as a force probe to measure the adhesion force between bacteria and biomaterials (viz. ultra-high molecu ... | 2017 | 27814559 |
| scaffolds for oriented and close-packed immobilization of immunoglobulins. | immunosensing is a widely used technique that detects the interactions between antibodies and antigens such as biochemical markers, pathogens, allergens, and tumor-associated antigens. since target antigens are often of high molecular mass and their binding affinities are sometimes weak, the spatial arrangement of immunoglobulin gs (iggs) on immunosensing probes should be optimized by presenting them in as close-packed a manner as possible and reducing steric hindrance around the antigen-binding ... | 2017 | 27818052 |
| evaluation of pharmacokinetic/pharmacodynamic indices of topical ophthalmic gatifloxacin against staphylococcus aureus utilising an in vitro aqueous humour pharmacokinetic model. | 2017 | 27817974 | |
| improvement of hand hygiene quality and compliance using bioburden measurement and online feedback in germany. | to improve compliance with hand hygiene, a novel method with inclusion of an online reporting system was developed, comprising measurement of total hand bioburden, anonymous online feedback, and onsite training. the intervention significantly improved both compliance and quality of hand hygiene and reduced staphylococcus aureus incidence. infect control hosp epidemiol 2016;1-4. | 2017 | 27817762 |
| ca-mrsa decolonization strategies: do they reduce recurrence rate? | community-associated methicillin-resistant staphylococcus aureus commonly presents as a skin and soft tissue infection. recurrence of the infection is common even after incision and drainage of the affected area. | 2017 | 27820584 |
| predicting maintenance doses of vancomycin for hospitalized patients undergoing hemodialysis. | methicillin-resistant staphylococcus aureus is a leading cause of death in patients undergoing hemodialysis. however, controversy exists about the optimal dose of vancomycin that will yield the recommended pre-hemodialysis serum concentration of 15-20 mg/l. | 2017 | 27826151 |
| sustained nitric oxide-releasing nanoparticles interfere with methicillin-resistant staphylococcus aureus adhesion and biofilm formation in a rat central venous catheter model. | staphylococcus aureus is frequently isolated in the setting of infections of indwelling medical devices, which are mediated by the microbe's ability to form biofilms on a variety of surfaces. biofilm-embedded bacteria are more resistant to antimicrobial agents than their planktonic counterparts and often cause chronic infections and sepsis, particularly in patients with prolonged hospitalizations. in this study, we demonstrate that sustained nitric oxide-releasing nanoparticles (no-np) interfere ... | 2017 | 27821454 |
| β-lactamase inhibitors enhance the synergy between β-lactam antibiotics and daptomycin against methicillin-resistant staphylococcus aureus. | the evidence for using combination therapy for the treatment of serious methicillin-resistant staphylococcus aureus (mrsa) infections is growing. in this study, we investigated the synergistic effect of daptomycin (dap) combined with piperacillin-tazobactam and ampicillin-sulbactam against mrsa in time-kill experiments. six of eight strains demonstrated synergy between dap and the β-lactam-β-lactamase inhibitor (bli) combination. in 5/8 strains, the synergy occurred only in the presence of the b ... | 2017 | 27821441 |
| bacterial replication rate modulation in combination with antimicrobial therapy: turning the microbe against itself. | a major clinical challenge for treating infectious diseases is the duration of antimicrobial therapy required to eradicate the pathogen. we hypothesized that modulation of the bacterial replication rate in the context of an antimicrobial exposure is coupled with the rate and extent of bactericidal effects. herein we describe results from in vitro infection model (one compartment, 24-h model; hollow fiber, 10-day model) studies designed to probe the relationship between the bacterial replication ... | 2017 | 27821440 |
| community- and healthcare-associated methicillin-resistant staphylococcus aureus strains: an investigation into household transmission, risk factors, and environmental contamination. | objective to measure transmission frequencies and risk factors for household acquisition of community-associated and healthcare-associated (ha-) methicillin-resistant staphylococcus aureus (mrsa). design prospective cohort study from october 4, 2008, through december 3, 2012. setting seven acute care hospitals in or near toronto, canada. participants total of 99 mrsa-colonized or mrsa-infected case patients and 183 household contacts. methods baseline interviews were conducted, and surveillance ... | 2017 | 27821194 |
| safety and efficacy of daptomycin in outpatient parenteral antimicrobial therapy: a prospective and multicenter cohort study (daptodom trial). | daptomycin is an optimal choice for outpatient parenteral antibiotic therapy (opat) because of its safety, once-daily administration and its activity against gram-positive bacteria. although daptomycin is increasingly being used in opat, limited information about its safety in this scenario is available. | 2017 | 27820968 |
| subdural empyema in the setting of multimodal intracranial monitoring. | multimodal intracranial monitoring is becoming an increasingly common tool in the management of patients with traumatic brain injury. although numerous reports detailing the benefits of such advanced monitoring exist in the literature, there is minimal discussion of the possible complications that may arise in this patient population. | 2017 | 27826090 |
| predominance of sccmec types iv and v among biofilm producing device-associated staphylococcus aureus strains isolated from tertiary care hospitals in mysuru, india. | device associated infections caused by staphylococcus aureus in hospitalised patients is a serious healthcare problem. the present study was designed to determine the prevalence of biofilm-producing mrsa in device-associated infections. | 2017 | 27825734 |
| novel hamamelitannin analogues for the treatment of biofilm related mrsa infections-a scaffold hopping approach. | antimicrobial research is increasingly being focused on the problem of resistance and biofilm formation. hamamelitannin (ham) was recently identified as an antimicrobial potentiator for conventional antibiotics towards staphylococcus aureus. this paper describes the synthesis and biological evaluation of novel hamamelitannin analogues with alternative central scaffolds. via a ligand-based approach, several interesting compounds with improved synthetic accessibility were identified as potentiator ... | 2017 | 27823882 |
| decreased hospital costs and surgical site infection incidence with a universal decolonization protocol in primary total joint arthroplasty. | staphylococcus aureus colonization has been identified as a key modifiable risk factor in the reduction of surgical site infections (ssi) related to elective total joint arthroplasty (tja). we investigated the incidence of ssis and cost-effectiveness of a universal decolonization protocol without screening consisting of nasal mupirocin and chlorhexidine before elective tja compared to a program in which all subjects were screened for s aureus and selectively treated if positive. | 2017 | 27823845 |
| staphylococcus aureus resistance to topical antimicrobials in atopic dermatitis. | topical antimicrobial drugs are indicated for limited superficial pyodermitis treatment, although they are largely used as self-prescribed medication for a variety of inflammatory dermatoses, including atopic dermatitis. monitoring bacterial susceptibility to these drugs is difficult, given the paucity of laboratory standardization. | 2017 | 27828633 |
| cylop-1: membrane-active peptide with cell-penetrating and antimicrobial properties. | cylop-1 is a cysteine-rich cell-penetrating peptide derived from nuclear localization sequence of snake toxin, crotamine. the peptide has shown cytoplasmic uptake in mammalian cells at lower concentrations. in the present study, the cell-penetrating and antimicrobial activity of the peptide has been studied by employing mammalian cells, plant cells as well as bacterial and fungal pathogens. the study shows that the peptide acts as an effective cpp and a cargo-delivery vector for not only mammali ... | 2017 | 27836642 |
| increased biofilm formation ability and accelerated transport of staphylococcus aureus along a catheter during reciprocal movements. | staphylococcus spp. is a major cause of device-related infections. however, the mechanisms of deep-tissue infection by staphylococci from the skin surface remain unclear. we performed in vitro experiments to determine how staphylococci are transferred from the surface to the deeper layers of agar along the catheter for different strains of staphylococcus aureus with respect to bacterial concentrations, catheter movements, and biofilm formation. we found that when 5-mm reciprocal movements of the ... | 2017 | 27836632 |
| inhibition of the multidrug efflux pump lmrs from staphylococcus aureus by cumin spice cuminum cyminum. | staphylococcus aureus is a serious causative agent of infectious disease. multidrug-resistant strains like methicillin-resistant s. aureus compromise treatment efficacy, causing significant morbidity and mortality. active efflux represents a major antimicrobial resistance mechanism. the proton-driven multidrug efflux pump, lmrs, actively exports structurally distinct antimicrobials. to circumvent resistance and restore clinical efficacy of antibiotics, efflux pump inhibitors are necessary, and n ... | 2017 | 27830269 |
| genetic lineages of methicillin-resistant staphylococcus aureus acquired during admission to an intensive care unit of a general hospital. | the objectives of this study were to determine the frequency of methicillin-resistant staphylococcus aureus (mrsa) colonization or infection while on admission to the intensive care unit (icu), and examine the genetic backgrounds of the mrsa isolates to establish transmission among the patients. | 2017 | 27829243 |
| antibiotic susceptibility of wound isolates in plastic surgery patients at a tertiary care centre. | wound infection increases the hospital stay and adversely affects the recovery of patients. culture and sensitivity of wound isolates help in proper diagnosis and management of these patients. | 2017 | 27833282 |
| linezolid versus vancomycin for nosocomial pneumonia due to methicillin-resistant staphylococcus aureus in the elderly: a retrospective cohort analysis: effectiveness of linezolid in the elderly. | several reports have compared the efficacy of linezolid (lzd) in methicillin-resistant staphylococcus aureus (mrsa) infections with that of vancomycin (vcm); however, these two antibiotics for the treatment of nosocomial mrsa pneumonia in elderly patients has not been well evaluated. the purpose of this study is to evaluate the efficacy and safety of lzd compared with vcm for the treatment of elderly patients with nosocomial mrsa pneumonia in a retrospective chart review of a cohort. | 2017 | 27832976 |
| periprosthetic infection following primary hip and knee arthroplasty: the impact of limiting the postoperative surveillance period. | background hip and knee arthroplasty infections are associated with considerable healthcare costs. the merits of reducing the postoperative surveillance period from 1 year to 90 days have been debated. objectives to report the first pan-canadian hip and knee periprosthetic joint infection (pji) rates and to describe the implications of a shorter (90-day) postoperative surveillance period. methods prospective surveillance for infection following hip and knee arthroplasty was conducted by hospital ... | 2017 | 27834161 |
| ifn-τ plays an anti-inflammatory role in staphylococcus aureus-induced endometritis in mice through the suppression of nf-κb pathway and mmp9 expression. | interferon-tau (ifn-τ) is a type i interferon and considered as a pregnancy recognition signal in ruminants. our previous reports have confirmed that ifn-τ has a potential anti-inflammatory effect in macrophage. however, the anti-inflammatory effect of ifn-τ on endometritis has never been reported. thus, the aim of this study was to investigate the effects of ifn-τ in a mouse model of staphylococcus aureus-induced endometritis. the histopathological and myeloperoxidase activity results showed th ... | 2017 | 27845845 |
| electrospun mupirocin loaded polyurethane fiber mats for anti-infection burn wound dressing application. | wound care treatment is a serious issue faced by the medical staffs due to its variety and complexity. wound dressings are typically used to manage the various types of wounds. in this study, polyurethane (pu) fibers containing mupirocin (mu), a commonly used antibiotic in wound care, were fabricated via electrospinning technique. the aim of this study was to develop biomedical electrospun fiber scaffolds for preventing wound infections with good compatibility and to demonstrate their applicatio ... | 2017 | 27848275 |
| pyomyositis caused by streptococcus pneumoniae after allogeneic hematopoietic stem cell transplantation. | pyomyositis is classified into two main types: tropical and non-tropical. non-tropical pyomyositis occurs among various immunocompromised patients, and staphylococcus aureus has been reported as the most common pathogen. pyomyositis caused by streptococcus pneumoniae is uncommon, and has not been previously reported after allogeneic hematopoietic stem cell transplantation (allo-hsct). here, we report a unique case with pyomyositis caused by s. pneumoniae in the bilateral erector spinae muscles 3 ... | 2017 | 27847295 |
| sinonasal methicillin-resistant staphylococcus aureus: updates on treatment. | over the past two decades, the management of methicillin-resistant staphylococcus aureus (mrsa) in chronic rhinosinusitis has posed significant challenges. this document reviews current management techniques and novel treatment modalities for sinonasal mrsa infections. | 2017 | 27841769 |
| critical potential of early cardiac surgery for infective endocarditis with cardio-embolic strokes. | early cardiac surgery may have a trade-off between stabilized hemodynamics with controlled infection and a risk of peri-operative death in patients with infective endocarditis (ie) complicated with cardio-embolic strokes. | 2017 | 27839820 |
| impact of chlorhexidine bathing on methicillin-resistant staphylococcus aureus incidence in an endemic chronic care setting: a randomized controlled trial. | we postulated that bathing with 2% chlorhexidine-impregnated antiseptic washcloths could reduce methicillin-resistant staphylococcus aureus (mrsa) incidence among chronic care patients compared with nonantiseptic bathing. a total of 122 patients on 3 hospital units were enrolled in a 12-month, cluster-randomized, open-label, controlled trial, with 8 patients becoming mrsa positive. the 2% chlorhexidine-impregnated antiseptic washcloths reduced incidence by 71% (0.1 vs 0.44 cases per 1,000 patien ... | 2017 | 27839752 |
| the synthesis and antistaphylococcal activity of dehydroabietic acid derivatives: modifications at c12 and c7. | a series of 7-n-acylaminoethyl/propyloxime derivatives of dehydroabietic acid were synthesized and investigated for their antibacterial activity against staphylococcus aureus newman strain and multidrug-resistant strains (nrs-1, nrs-70, nrs-100, nrs-108 and nrs-271). most of the target compounds having trifluoromethyl phenyl/benzyl, halogen-substituted thiophenyl, benzothiophenyl or pyrrolyl moiety exhibited potent in vitro antibacterial activity. among which, compounds 4m, 4x and 7j showed high ... | 2017 | 27837995 |
| short communication: outbreak of methicillin-resistant staphylococcus aureus (mrsa)-associated mastitis in a closed dairy herd. | cows are probably the main source of contamination of raw milk with staphylococcus aureus. mammary glands with subclinical mastitis can shed large numbers of staph. aureus in milk. because of the risk of this pathogen to human health as well as animal health, the aim of this paper was to describe an outbreak of mastitis caused by methicillin-resistant staph. aureus (mrsa), oxacillin-susceptible meca-positive staph. aureus (os-mrsa), and methicillin-susceptible staph. aureus (mssa) on a dairy far ... | 2017 | 27837983 |
| antimicrobial activity of novel c2-substituted 1,4-dihydropyridine analogues. | the antimicrobial activity of 3-methyl-5-isopropyl (or ethyl) 6-methyl-4-nitrophenyl-1,4-dihydropyridine-3,5-dicarboxylate derivatives was evaluated. prokaryotes (bacteria) appeared to be more sensitive to their antimicrobial activity than were eukaryotes (filamentous fungi). the best antibacterial activity was shown by derivative 33, which was able to inhibit the growth of mycobacterium smegmatis (mic33 = 9 μg.ml(-1)), staphylococcus aureus (mic33 = 25 μg.ml(-1)), and escherichia coli (mic33 = ... | 2017 | 24959401 |
| antibacterial and antispasmodic activities of a dichloromethane fraction of an ethanol extract of stem bark of piliostigma reticulatum. | this study presents the antispasmodic and antibacterial properties of an ethanol extract and fractions the of stem bark of piliostigma reticulatum. | 2017 | 25883517 |
| antimicrobial resistance of bacterial isolates from respiratory secretions of ventilated patients in a multi-specialty hospital. | ventilator-associated pneumonia (vap) is a common nosocomial infection occurring in intensive care unit (icu) settings. vap occurs due to interplay of three factors - impaired host defense, access of large numbers of pathogenic bacteria to the lower respiratory tract and the virulence of the organism. knowledge of colonizing microbial flora and their antibiogram in ventilated patients is of great importance in timely institution of empirical therapy, so that mortality and morbidity due to vap ca ... | 2017 | 26229758 |
| observations on the current bacteriological profile of chronic suppurative otitis media in south eastern nigeria. | chronic suppurative otitis media (csom) is a disease well-known for its recurrence and persistence despite treatment. the situation is compounded by the increasing resistance to antimicrobial agents by bacteria these days. | 2017 | 25861532 |
| evaluation of the antibacterial activity of the althaea officinalis l. leaf extract and its wound healing potency in the rat model of excision wound creation. | wound is defined simply as the disruption of the biochemical, cellular, and anatomic continuity of a tissue. plants and their extracts known as phytomedicine have immense potential for the management and treatment of wounds. | 2017 | 25949951 |
| synthesis of norbornane bisether antibiotics via silver-mediated alkylation. | a small series of norbornane bisether diguanidines have been synthesized and evaluated as antibacterial agents. the key transformation-bisalkylation of norbornane diol 6-was not successful using williamson methodology but has been accomplished using ag2o mediated alkylation. further functionalization to incorporate two guanidinium groups gave rise to a series of structurally rigid cationic amphiphiles; several of which (16d, 16g and 16h) exhibited antibiotic activity. for example, compound 16d w ... | 2017 | 26251697 |
| bacteriological profile of neonatal septicemia in a tertiary care hospital from western india. | neonatal septicemia is an important cause of morbidity and mortality. the present study was undertaken to determine the bacteriological profile and antimicrobial susceptibility pattern of prevalent pathogens isolated from the blood of septicemic neonates from neonatal intensive care unit (nicu). a total of 180 blood samples of septicemic neonates were studied bacteriologically. antimicrobial susceptibility testing was done by the kirby bauer disc diffusion method in accordance to clinical labora ... | 2017 | 26069427 |
| bloodstream infections among children with juvenile idiopathic arthritis: a prospective study from the onset of disease. | to describe the incidence and nature of bloodstream infections (bsi) among children with juvenile idiopathic arthritis (jia) followed-up prospectively from disease onset. | 2017 | 25436839 |
| community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients. | mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ett) biofilms. for the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, etts, and non-directed bronchial lavages (nbls) in mechanically ventilated patients to determine similarities in respective microbial commun ... | 2017 | 28259058 |
| methicillin-resistant staphylococcus aureus as a cause of lemierre's syndrome. | lemierre's syndrome (ls) or jugular vein suppurative thrombophlebitis is well described in literature. the organisms most often responsible are fusobacterium necrophorum or anaerobic flora. we present a case of ls with an atypical microbiologic cause, methicillin-resistant staphylococcus aureus. we also present retrospective review of all ls cases from our institution and identified 2 additional children with ls caused by methicillin-resistant s. aureus. | 2017 | 27977559 |
| benzimidazole-based fabi inhibitors: a promising novel scaffold for anti-staphylococcal drug development. | the enoyl-acp reductase (fabi) enzyme is a well validated target for anti-staphylococcal drug discovery and development. with the goal of finding alternate therapeutics for drug-resistant strains of staphylococcus aureus, such as methicillin-resistant s. aureus (mrsa), our previously published series of benzimidazole-based inhibitors of the fabi enzyme from francisella tularensis (ftfabi) have been evaluated against fabi from s. aureus (safabi). we report here the preliminary structure-activity ... | 2017 | 27756129 |
| prevention of influenza virus induced bacterial superinfection by standardized echinacea purpurea, via regulation of surface receptor expression in human bronchial epithelial cells. | viral infections may predispose the airways to secondary bacterial infections that can lead to unfavorable progression of principally self-limiting illnesses. such complicated respiratory infections include pneumonia, bronchitis, sinusitis, acute otitis media, and sepsis, which cause high morbidity and lethality. some of the pathogenic consequences of viral infections, like the expression of bacterial adhesion receptors and the disturbance of physical barrier integrity due to inflammation, may c ... | 2017 | 28279802 |
| a case of influenza a (h3n2) complicated by community-acquired pneumonia and death in a young healthy adult during the 2013-2014 season. | with multiple available vaccines and antivirals, seasonal influenza a is typically a self-limited acutely debilitating illness in young healthy adults. here, we illustrate unexpected morbidity and mortality in a relatively young and healthy patient seen at a large tertiary care academic medical center for seasonal influenza a (h3n2) complicated by community-acquired pneumonia, hypoxic respiratory failure, septic shock, and death. | 2017 | 28229066 |
| concordance between upper and lower airway microbiota in infants with cystic fibrosis. | nasopharyngeal and oropharyngeal samples are commonly used to direct therapy for lower respiratory tract infections in non-expectorating infants with cystic fibrosis (cf).we aimed to investigate the concordance between the bacterial community compositions of 25 sets of nasopharyngeal, oropharyngeal and bronchoalveolar lavage (bal) samples from 17 infants with cf aged ∼5 months (n=13) and ∼12 months (n=12) using conventional culturing and 16s-rrna sequencing.clustering analyses demonstrated that ... | 2017 | 28356374 |
| diagnostic value of bronchoalveolar lavage in children with nonresponding community-acquired pneumonia. | community-acquired pneumonia (cap) is a common cause of morbidity and mortality in hospitalized children. in cap, causative agents are seldom identified using noninvasive diagnostic procedures. for those children not responding to empiric antibiotic therapy, it is vital to identify the causative pathogens for further management. | 2017 | 28351556 |
| retrospective report of antimicrobial susceptibility observed in bacterial pathogens isolated from ocular samples at mount sinai hospital, 2010 to 2015. | antimicrobial resistance has emerged as a major threat to global public health. thus, the surveillance of changes in antimicrobial resistance in local and global settings is a paramount necessity. while many studies have tracked antimicrobial resistance, only a small percentage surveyed ocular isolates. the purpose of this study was to report the in vitro susceptibility of bacterial pathogens isolated from ocular samples in new york, ny from 2010 to 2015. | 2017 | 28344783 |
| discharge and infection in retinoblastoma post-enucleation sockets. | to investigate the causes and treatment options for socket discharge and infection in patients enucleated for retinoblastoma (rb). | 2017 | 28280296 |
| design, synthesis and structure-bactericidal activity relationships of novel 9-oxime ketolides and reductive epimers of acylides. | erythromycin was long viewed as a bacteriostatic agent. the erythromycin derivatives, 9-oxime ketolides have a species-specific bactericidal profile. among them, the 3'-allyl version of the 9-oxime ketolide 1 (ar=3-quinolyl; 17a) is bactericidal against streptococcus pneumoniae and streptococcus pyogenes. in contrast, the 2-fluoro analogs of 1, 13a (ar=6-quinolyl), 13b (ar=3-quinolyl) and 24a (ar=4-isoquinolyl), show bactericidal activities against s. pneumoniae, staphylococcus aureus and moraxe ... | 2017 | 28256375 |
| simultaneous detection of nine key bacterial respiratory pathogens using luminex xtag(®) technology. | early diagnosis and treatment are crucial to the outcome of lower respiratory tract infections (lrtis). in this study, we developed an assay combining multiplex pcr and luminex technology (mplt) for the detection of nine important respiratory bacterial pathogens, which frequently cause lrtis. these were streptococcus pneumoniae, moraxella catarrhalis, staphylococcus aureus, streptococcus pyogenes, haemophilus influenzae, mycoplasma pneumoniae, legionella spp., pseudomonas aeruginosa, and klebsie ... | 2017 | 28241513 |
| evaluation of antibacterial potential and toxicity of plant volatile compounds using new broth microdilution volatilization method and modified mtt assay. | with aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. in addition, eva (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (mtt) assay for cytoto ... | 2017 | 28223069 |
| epidemiology of otitis media with spontaneous perforation of the tympanic membrane in young children and association with bacterial nasopharyngeal carriage, recurrences and pneumococcal vaccination in catalonia, spain - the prospective hermes study. | the epidemiology of otitis media with spontaneous perforation of the tympanic membrane and associated nasopharyngeal carriage of bacterial otopathogens was analysed in a county in catalonia (spain) with pneumococcal conjugate vaccines (pcvs) not included in the immunization programme at study time. a prospective, multicentre study was performed in 10 primary care centres and 2 hospitals (june 2011-june 2014), including all otherwise healthy children ≥2 months ≤8 years with otitis media presentin ... | 2017 | 28146590 |
| prospective evaluation of the aetiology of acute otitis media with spontaneous tympanic membrane perforation. | to evaluate the aetiological role of the main bacterial pathogens associated with acute otitis media (aom) in children with aom and spontaneous tympanic membrane perforation (stmp). | 2017 | 28110050 |
| nasopharyngeal carriage of respiratory pathogens in warao amerindians: significant relationship with stunting. | to assess risk factors for nasopharyngeal carriage of potential pathogens in geographically isolated warao amerindians in venezuela. | 2017 | 28072501 |
| multiplex pcr reveals that viruses are more frequent than bacteria in children with cystic fibrosis. | cystic fibrosis is a degenerative disease characterized by progressive epithelial secretory gland dysfunction associated with repeated respiratory infections. bacterial infections are very frequent in children with cystic fibrosis, but because rapid methods: for screening for the wide variety of potentially involved viruses were unavailable until recently, the frequency of viral presence is unknown. multiplex pcr enables screening for many viruses involved in respiratory infections. | 2017 | 27886635 |
| staphylococcus aureus carriage at admission predicts early-onset pneumonia after burn trauma. | early-onset pneumonia (eop) is frequent after burn trauma, increasing morbidity in the critical resuscitation phase, which may preclude early aggressive management of burn wounds. currently, however, preemptive treatment is not recommended. the aim of this study was to identify predictive factors for eop that may justify early empirical antibiotic treatment. data for all burn patients requiring ≥4 h mechanical ventilation (mv) who were admitted between january 2001 and october 2012 were extracte ... | 2017 | 27815777 |
| low prevalence of kingella kingae carriage in children aged 6-48 months in sydney, australia. | a prospective observational study was conducted to estimate the prevalence of oropharyngeal carriage of kingella kingae in healthy australian pre-school children. | 2017 | 27669685 |
| hemodialysis catheter heat transfer for biofilm prevention and treatment. | central line-associated bloodstream infections (clabsis) are not easily treated, and many catheters (e.g., hemodialysis catheters) are not easily replaced. biofilms (the source of infection) on catheter surfaces are notoriously difficult to eradicate. we have recently demonstrated that modest elevations of temperature lead to increased staphylococcal susceptibility to vancomycin and significantly soften the biofilm matrix. in this study, using a combination of microbiological, computational, and ... | 2017 | 26501916 |
| preoperative evaluation of micro-organisms in non-operated cleft in soft palate: impact on use of antibiotics. | to identify the pathogenic micro-organisms that had colonised preoperatively in clefts in the soft palate and oro-nasopharynx, we retrospectively studied the preoperative microbiological profiles of 200 infants who had had primary repair of all types of cleft in the soft palate. data from a private practice that specialises in the repair of facial clefts were extracted randomly from patients' files. we analysed the results of the culture of preoperative swabs taken from clefts in the soft palate ... | 2017 | 27745784 |
| how often do clinically diagnosed catheter-associated urinary tract infections in nursing homes meet standardized criteria? | to determine the relationship between clinically diagnosed catheter-associated urinary tract infection (cauti) and standardized criteria and to assess microorganism-level differences in symptom burden in a cohort of catheterized nursing home (nh) residents. | 2017 | 27858954 |
| antibody-free detection of infectious bacteria using quantum dots-based barcode assay. | staphylococcus aureus, methicillin-resistant staphylococcus aureus and klebsiella pneumoniae are the most representative bacteria causing infectious diseases. due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. therefore, a sensitive determination of these pathogens is crucial for effective treatment. the aim of this study was to design an effective method for multiplex detection of staphylococcus aureus, methicillin-resistant staphy ... | 2017 | 27894780 |
| virulence and antimicrobial resistance of common urinary bacteria from asymptomatic students of niger delta university, amassoma, bayelsa state, nigeria. | asymptomatic bacteriuria frequently occurs among all ages with the possibility of developing into urinary tract infections, and the antimicrobial resistance patterns of the etiologic organisms are essential for appropriate therapy. thus, we investigated the virulence and antimicrobial resistance patterns of common urinary bacteria in asymptomatic students of niger delta university, amassoma, bayelsa state, nigeria in a cross-sectional study. | 2017 | 26957865 |
| participation of necroptosis in the host response to acute bacterial pneumonia. | common pulmonary pathogens, such as streptococcus pneumoniae and staphylococcus aureus, as well as the host-adapted pathogens responsible for health care-associated pneumonias, such as the carbapenem-resistant klebsiella pneumoniae and serratia marcecsens, are able to activate cell death through the ripk1/ripk3/mlkl cascade that causes necroptosis. necroptosis can influence the pathogenesis of pneumonia through several mechanisms. activation of this pathway can result in the loss of specific typ ... | 2017 | 28125817 |
| green production of microalgae-based silver chloride nanoparticles with antimicrobial activity against pathogenic bacteria. | silver nanoparticles are powerful antimicrobial agents. here, the synthesis of silver chloride nanoparticles (agcl-nps) was consistently evidenced from a commercially valuable microalgae species, chlorella vulgaris. incubation of c. vulgaris conditioned medium with agno3 resulted in a medium color change to yellow/brown (with uv-vis absorbance at 415nm), indicative of silver nanoparticle formation. energy-dispersive x-ray spectroscopy (eds) of purified nanoparticles confirmed the presence of bot ... | 2017 | 28010768 |