Publications

miltefosine is fungicidal to paracoccidioides spp. yeast cells but subinhibitory concentrations induce melanisation.	paracoccidioidomycosis (pcm) is a systemic mycosis caused by the dimorphic fungi paracoccidioides spp. the duration of antifungal treatment ranges from months to years and relapses may nevertheless occur despite protracted therapy. thus, there remains an urgent need for new therapeutic options. miltefosine (mlt), an analogue of alkylphospholipids, has antifungal activity against species of yeast and filamentous fungi. the aim of this study was to evaluate the antifungal effects of mlt on the yea ...	2017	28279786
standardization and validation of dot-elisa assay for paracoccidioides brasiliensis antibody detection.	paracoccidioidomycosis (pcm) is a neglected systemic mycosis caused by a dimorphic fungus of the paracoccidioides genus. the standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. however, in some situations, access to the site of injury prevents the collection of biological material. a variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a ...	2017	28239394
effects of two 6-quinolinyl chalcones on the integrity of plasma membrane of paracoccidioides brasiliensis.	paracoccidioidomycosis is the most prevalent systemic mycosis in latin america, yet few therapeutic options exist. our aim was to search for new compounds with high efficacy, low toxicity, shorter treatment time and affordable cost. we studied two synthetic 6-quinolinyl chalcones, 3b and 3e, to determine their effects on vero cells, antifungal activity, survival curve, interaction with other drugs and phenotypic effects against several isolates of paracoccidioides spp. in this study, we verified ...	2017	28074055
phosphosite-specific regulation of the oxidative-stress response of paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis.	paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in latin america. this fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. therefore, the survival of p. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. for several years, reactive oxygen species (ros) ...	2017	27590702
linear epitopes of paracoccidioides brasiliensis and other fungal agents of human systemic mycoses as vaccine candidates.	dimorphic fungi are agents of systemic mycoses associated with significant morbidity and frequent lethality in the americas. among the pathogenic species are paracoccidioides brasiliensis and paracoccidioides lutzii, which predominate in south america; histoplasma capsulatum, coccidioides posadasii, and coccidioides immitis, and the sporothrix spp. complex are other important pathogens. associated with dimorphic fungi other important infections are caused by yeast such as candida spp. and crypto ...	2017	28344577
use of fluorescent oligonucleotide probes for differentiation between paracoccidioides brasiliensis and paracoccidioides lutzii in yeast and mycelial phase.	fluorescence in situ hybridisation (fish) associated with tyramide signal amplification (tsa) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture.	2017	28177048