Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cost-effectiveness of using an extensively hydrolyzed casein formula plus the probiotic lactobacillus rhamnosus gg compared to an extensively hydrolyzed formula alone or an amino acid formula as first-line dietary management for cow's milk allergy in the us. | the aim was to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula (ehcf) plus the probiotic lactobacillus rhamnosus gg (ehcf + lgg; nutramigen lgg) compared to an ehcf alone (nutramigen) and an amino acid formula (aaf; neocate) as first-line dietary management for cow's milk allergy (cma) in the us. | 2015 | 25767400 |
| a possible link between early probiotic intervention and the risk of neuropsychiatric disorders later in childhood: a randomized trial. | recent experimental evidence suggests that gut microbiota may alter function within the nervous system providing new insight on the mechanism of neuropsychiatric disorders. | 2015 | 25760553 |
| inhibition of mir122a by lactobacillus rhamnosus gg culture supernatant increases intestinal occludin expression and protects mice from alcoholic liver disease. | alcoholic liver disease (ald) has a high morbidity and mortality. chronic alcohol consumption causes disruption of intestinal microflora homeostasis, intestinal tight junction barrier dysfunction, increased endotoxemia, and eventually liver steatosis/steatohepatitis. probiotic lactobacillus rhamnosus gg (lgg) and the bacteria-free lgg culture supernatant (lggs) have been shown to promote intestinal epithelial integrity and protect intestinal barrier function in ald. however, little is known abou ... | 2015 | 25746479 |
| non-viable immunobiotic lactobacillus rhamnosus crl1505 and its peptidoglycan improve systemic and respiratory innate immune response during recovery of immunocompromised-malnourished mice. | the effect of non-viable lactobacillus rhamnosus crl1505 and its cell wall and peptidoglycan on respiratory immunity in malnourished mice was studied. weaned mice were malnourished with a protein-free diet for 21d and received bcd during 7d (bcd) or bcd with nasal non-viable l. rhamnosus crl1505 (bcd+uv) or its cell wall (bcd+cw) or peptidoglycan (bcd+pg) supplementation during last 2d of the treatment. malnourished mice without treatment (mnc) and well-nourished mice (wnc) were used as controls ... | 2015 | 25744605 |
| probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052) reduce the expression of toll-like receptor 4 in mice with alcoholic liver disease. | the role of lipopolysaccharide (lps) and toll-like receptor 4 (tlr 4) in the pathogenesis of alcoholic liver disease (ald) has been widely established. we evaluated the biological effects of probiotics (lactobacillus rhamnosus r0011 and acidophilus r0052), krg (korea red ginseng), and urushiol (rhus verniciflua stokes) on ald, including their effects on normal and high-fat diet in mice. | 2015 | 25692549 |
| nonpharmacologic treatment of functional abdominal pain disorders: a systematic review. | various nonpharmacologic treatments are available for pediatric abdominal pain-related functional gastrointestinal disorders (ap-fgids). data on efficacy and safety are scarce. the goal of this study was to summarize the evidence regarding nonpharmacologic interventions for pediatric ap-fgids: lifestyle interventions, dietary interventions, behavioral interventions, prebiotics and probiotics, and alternative medicine. | 2015 | 25667239 |
| [irritable bowel syndrome; gut microbiota and probiotic therapy]. | irritable bowel syndrome (ibs) is characterized by symptoms of abdominal pain and altered bowel habits. this common disorder is managed by varying clinical styles as no dominant therapeutic strategy has emerged. the pathophysiology of ibs remains unknown, but several lines of evidence link this disorder with the gut microbiota. although controversy exists, gut microbiota is likely contributing to symptoms of ibs, at least in some patients, through an altered fermentation process, an impaired int ... | 2015 | 25659060 |
| [probiotics for the treating acute diarrhea and preventing antibiotic-associated diarrhea in children]. | probiotics are helpful in the treatment of acute diarrhea. several systematic reviews show that the use of probiotics shortens the duration of diarrhea in one day and reduces by 59% the risk of diarrhea lasting longer than 4 days. hospital stay is 24 hours shorter in children treated with probiotics. the benefitial effect of probiotics is species-specific; lactobacillus rhamnosus gg and saccharomyces boulardii are the two species with proven efficacy in the treatment of acute diarrhea. the use o ... | 2015 | 25659056 |
| fermented red ginseng extract inhibits cancer cell proliferation and viability. | red ginseng (panax ginseng c.a. meyer) is the most widely recognized medicinal herb due to its remedial effects in various disorders, such as cancers, diabetes, and heart problems. in this study, we investigated the anticancer effect of fermented red ginseng extract (f-rge; provided by jeonju biomaterials institute, jeonju, south korea) in a parallel comparison with the effect of nonfermented red ginseng extract (nf-rge; control) on several cancer cell lines--mcf-7 breast cancer cells, hepg2 hep ... | 2015 | 25658580 |
| influence of orally administered lactobacillus gg on respiratory immune response in a murine model of diet-induced obesity. | mice with diet-induced obesity were fed with lactobacillus rhamnosus gg (lgg) suspended in saline or saline alone (control mice). pulmonary mrna expression of ifn-γ; ifn-α receptor 1; cd247 antigen; killer cell lectin-like receptor subfamily k, member 1; tnf-α; il-12 receptor β1 and il-2 receptor β, and the proportion of lactobacillales in feces were significantly greater in the lgg group than in the control mice (p < 0.05 and p < 0.01, respectively). these results suggest that lgg alters the re ... | 2015 | 25643737 |
| modulatory activity of lactobacillus rhamnosus oll2838 in a mouse model of intestinal immunopathology. | gut microbiota and probiotic strains play an important role in oral tolerance by modulating regulatory and effector cell components of the immune system. we have previously described the ability of lactobacilli to influence both the innate and adaptive immunity to wheat gluten, a food antigen, in mouse. in this study, we further explored the immunomodulatory mechanisms elicited in this model by testing three specific probiotic strains, namely l. rhamnosus oll2838, b. infantis atcc15697 and s. th ... | 2015 | 25623030 |
| enhanced ampk phosphorylation contributes to the beneficial effects of lactobacillus rhamnosus gg supernatant on chronic-alcohol-induced fatty liver disease. | we have previously demonstrated that lactobacillus rhamnosus gg culture supernatant (lggs) prevents acute-alcohol-exposure-induced hepatic steatosis and injury. the protective effects of lggs were attributed to the improved intestinal barrier function leading to decreased endotoxemia. the purpose of this study was to determine whether lggs was effective in protecting against chronic-alcohol-induced hepatic steatosis and injury and to evaluate the underlying mechanisms of lggs on hepatic lipid me ... | 2015 | 25622859 |
| administration of a probiotic with peanut oral immunotherapy: a randomized trial. | coadministration of a bacterial adjuvant with oral immunotherapy (oit) has been suggested as a potential treatment for food allergy. | 2015 | 25592987 |
| hepatic and fecal metabolomic analysis of the effects of lactobacillus rhamnosus gg on alcoholic fatty liver disease in mice. | the interactions among the gut, liver, and immune system play an important role in liver disease. probiotics have been used for the treatment and prevention of many pathological conditions, including liver diseases. comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (gc×gc-tof ms) was used herein, in conjunction with chemometric data analysis, to identify metabolites significantly affected by probiotics in mice fed with or without alcohol. the metabolomics analysis ... | 2015 | 25592873 |
| lactobacillus rhamnosus gg and its spac pilus adhesin modulate inflammatory responsiveness and tlr-related gene expression in the fetal human gut. | bacterial contact in utero modulates fetal and neonatal immune responses. maternal probiotic supplementation reduces the risk of immune-mediated disease in the infant. we investigated the immunomodulatory properties of live lactobacillus rhamnosus gg and its spac pilus adhesin in human fetal intestinal models. | 2015 | 25580735 |
| piliation of lactobacillus rhamnosus gg promotes adhesion, phagocytosis, and cytokine modulation in macrophages. | recently, spacba-encoded pili on the cell surface of lactobacillus rhamnosus gg were identified to be key molecules for binding to human intestinal mucus and caco-2 intestinal epithelial cells. here, we investigated the role of the spacba pilus of l. rhamnosus gg in the interaction with macrophages in vitro by comparing the wild type with surface mutants. our results show that spacba pili play a significant role in the capacity for adhesion to macrophages and also promote bacterial uptake by the ... | 2015 | 25576613 |
| lactobacillus rhamnosus gg dosage affects the adjuvanticity and protection against rotavirus diarrhea in gnotobiotic pigs. | the use of immunostimulatory strains of probiotics as adjuvants has been increasingly recognized as a promising approach in enhancing vaccine immunogenicity; however, dose effects of probiotic adjuvants are not well defined. in the present study, we examined dose effects of a commonly used probiotic strain, lactobacillus rhamnosus gg (lgg), on immunomodulation with 2 different dosages. | 2015 | 25564808 |
| microencapsulation of alginate-immobilized bagasse with lactobacillus rhamnosus nrrl 442: enhancement of survivability and thermotolerance. | the aim of this research was to enhance the survivability of lactobacillus rhamnosus nrrl 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (sb) and sodium alginate (naa), respectively. the microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and naa:sb ratio of 1:0, 1:1 and 1:1.5. this beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation ... | 2015 | 25563958 |
| production of lactic acid from the mixture of softwood pre-hydrolysate and paper mill sludge by simultaneous saccharification and fermentation. | paper mill sludge is a solid waste material composed of pulp residues and ash generated from pulping and paper making process. the carbohydrate portion of the sludges from kraft/recycle paper mill has chemical and physical characteristics similar to those of commercial wood pulp. because of its high carbohydrate content and well-dispersed structure, the sludge can be biologically converted to value-added products without pretreatment. in bioconversion of solid feedstock such as paper mill sludge ... | 2015 | 25561054 |
| [susceptibility of lactobacillus rhamnosus strains isolated from probiotic products to antimicrobial agents]. | probiotics generally recognized as safe (gras), when given in relevant dose, are able to induce strain-specific beneficial effects for health of humans or animals. | 2015 | 27019914 |
| uncovering surface-exposed antigens of lactobacillus rhamnosus by cell shaving proteomics and two-dimensional immunoblotting. | the present study reports the identification and comparison of all expressed cell-surface exposed proteins from the well-known probiotic l. rhamnosus gg and a related dairy strain, lc705. to obtain this information, the cell-surface bound proteins were released from intact cells by trypsin shaving under hypertonic conditions with and without dtt. liquid chromatography tandem mass spectrometry (lc-ms/ms) analyses of the purified peptides identified a total of 102 and 198 individual proteins from ... | 2015 | 25531588 |
| physicochemical characterization of exopolysaccharides produced by lactobacillus rhamnosus on various carbon sources. | the impact of five carbohydrate sources (glucose, maltose, galactose, sucrose, and lactose) on the chemical composition, structure, morphology, and physicochemical properties, as well as, viscosity of exopolysaccharides (epss) produced by lactobacillus rhamnosus e/n was investigated. glc-ms analysis and 2dnmr spectroscopy showed that the epss had the same primary structure independently of the carbon source used in the growth medium. the following eps composition was elucidated: four rhamnose, t ... | 2015 | 25498664 |
| characterization of the antifungal activity of lactobacillus harbinensis k.v9.3.1np and lactobacillus rhamnosus k.c8.3.1i in yogurt. | few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. consumer's demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. in this study, using a 2(5-1) factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contaminati ... | 2015 | 25481057 |
| the use of flow cytometry to accurately ascertain total and viable counts of lactobacillus rhamnosus in chocolate. | the goals of this study were to evaluate the precision and accuracy of flow cytometry (fc) methodologies in the evaluation of populations of probiotic bacteria (lactobacillus rhamnosus r0011) in two commercial dried forms, and ascertain the challenges in enumerating them in a chocolate matrix. fc analyses of total (fc(t)) and viable (fc(v)) counts in liquid or dried cultures were almost two times more precise (reproducible) than traditional direct microscopic counts (dcm) or colony forming units ... | 2015 | 25475282 |
| effects of hot-water extracts from ganoderma lucidum residues and solid-state fermentation residues on prebiotic and immune-stimulatory activities in vitro and the powdered residues used as broiler feed additives in vivo. | large amounts of ganoderma lucidum (gl) commercial products are provided in the worldwide market such as powders, tea bags, or capsules as dietary supplements which contained triterpenoids and/or polysaccharides. therefore, it was estimated that several thousand tons of gl residues (glr) are produced and discarded. for recycling uses, the aim of this study was to evaluate the benefits of two hot-water extracts from glr (hwp_glr) and solid-state fermentation glr inoculated with gl mycelia (hwp_gl ... | 2015 | 28510826 |
| capability of exopolysaccharide-producing lactobacillus paraplantarum bgcg11 and its non-producing isogenic strain nb1, to counteract the effect of enteropathogens upon the epithelial cell line ht29-mtx. | the putative protective role of the exopolysaccharide (eps)-producing lactobacillus paraplantarum bgcg11, and its non-eps-producing isogenic strain nb1, was tested upon ht29-mtx monolayers challenged with seven opportunistic pathogens. the probiotic strain lactobacillus rhamnosus lmg18243 (gg) was used as a reference bacterium. tested lactobacilli were able to efficiently reduce the attachment to ht29-mtx of most pathogens. lb. paraplantarum nb1 and lb. rhamnosus gg were more efficient reducing ... | 2015 | 28411984 |
| targeting virulence: salmochelin modification tunes the antibacterial activity spectrum of β-lactams for pathogen-selective killing of escherichia coli. | new antibiotics are required to treat bacterial infections and counteract the emergence of antibiotic resistance. pathogen-specific antibiotics have several advantages over broad-spectrum drugs, which include minimal perturbation to the commensal microbiota. we present a strategy for targeting antibiotics to bacterial pathogens that utilises the salmochelin-mediated iron uptake machinery of gram-negative escherichia coli. salmochelins are c-glucosylated derivatives of the siderophore enterobacti ... | 2015 | 28717471 |
| influence of manufacturing processes on cell surface properties of probiotic strain lactobacillus rhamnosus lcr35®. | the influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. in the present study, we comparatively assessed the cell wall characteristics of the probiotic strain lactobacillus rhamnosus lcr35® together with three of its commercial formulations with intestinal applications. putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of l. rhamnosus lcr35®. a tot ... | 2015 | 25280746 |
| adhesion properties of lactobacillus rhamnosus mucus-binding factor to mucin and extracellular matrix proteins. | we previously described potential probiotic lactobacillus rhamnosus strains, isolated from fermented mare milk produced in sumbawa island, indonesia, which showed high adhesion to porcine colonic mucin (pcm) and extracellular matrix (ecm) proteins. recently, mucus-binding factor (mbf) was found in the gg strain of l. rhamnosus as a mucin-binding protein. in this study, we assessed the ability of recombinant mbf protein from the fsmm22 strain, one of the isolates of l. rhamnosus from fermented su ... | 2015 | 25351253 |
| lactobacillus rhamnosus gg reverses insulin resistance but does not block its onset in diet-induced obese mice. | recently, lactobacillus rhamnosus gg (lgg) was shown to exert insulin-sensitizing and adiposity-reducing effects in high-fat (hf) diet-fed mice. in the present study, we observed that the effects were correlated with the extent of dysbiosis induced by hf diet feeding before lgg administration. lgg-treated mice were protected from hf diet-induced adiposity and/ or insulin resistance when lgg was treated after, not along with, hf diet feeding. results indicate that, under hf dietary condition, sup ... | 2015 | 25433553 |
| brain and liver fatty acid composition changes upon consumption of lactobacillus rhamnosus la68. | recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. there are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. this study was designed to test the effects of a particular lactobacillus strain, lactobacillus rhamnosus la68 on fatty acid composition of the liver and the brain of c57bl/6 mice in the absence of an underlying pat ... | 2015 | 25431181 |
| efficacy of corn silage inoculants on the fermentation quality under farm conditions and their influence on aspergillus parasitucus, a. flavus and a. fumigatus determined by q-pcr. | laboratory-scale silos were prepared to evaluate the efficacy of two different lactic acid bacteria (lab) on the fermentation quality and mycobiota of corn silage. their influence on aspergillus species' variability by using the q-pcr technique was studied. silage inoculated with lactobacillus rhamnosus rc007 or l. plantarum rc009 were compared with uninoculated silage. silos were opened after 1, 7, 45, 90 and 120 days after ensiling. at the end of the ensiling period, silos were left open for 7 ... | 2015 | 25421370 |
| effect of lactobacillus rhamnosus hsryfm 1301 on the gut microbiota and lipid metabolism in rats fed a high-fat diet. | accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. to investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed using real-time pcr before and after administration of lactobacillus rhamnosus hsryfm 1301 and its fermented milk for 28 days. the findings showed that the lactobacillus spp., bifi ... | 2015 | 25418480 |
| probiotics: an update. | triggered by the growing knowledge on the link between the intestinal microbiome and human health, the interest in probiotics is ever increasing. the authors aimed to review the recent literature on probiotics, from definitions to clinical benefits, with emphasis on children. | 2015 | 25458874 |
| molecular characteristics of an exopolysaccharide from lactobacillus rhamnosus kf5 in solution. | the molecular weight and chain conformation of an exopolysaccharide (eps), s2, from lactobacillus rhamnosus kf5 were determined by size-exclusion chromatography combined with multi-angle laser light scattering (sec-malls), dynamic light scattering (dls), viscometry and atomic force microscopy (afm). the weight-average molecular weight (mw), intrinsic viscosity [ƞ], radii of gyration (r(g)) and hydrodynamic radii (r(h)) were 7.5 × 10(5) da, 2.331 dl/g, 44.3 nm and 29.4 nm, respectively. the confo ... | 2015 | 25453290 |
| lactobacillus rhamnosus gg lysate increases re-epithelialization of keratinocyte scratch assays by promoting migration. | a limited number of studies have investigated the potential of probiotics to promote wound healing in the digestive tract. the aim of the current investigation was to determine whether probiotic bacteria or their extracts could be beneficial in cutaneous wound healing. a keratinocyte monolayer scratch assay was used to assess re-epithelialization; which comprises keratinocyte proliferation and migration. primary human keratinocyte monolayers were scratched then exposed to lysates of lactobacillu ... | 2015 | 26537246 |
| effect of the probiotic lactobacillus rhamnosus lb21 on the cariogenicity of streptococcus mutans ua159 in a dual-species biofilm model. | despite promising results using probiotics, evidence of the preventive effect on enamel demineralization is insufficient and the cariogenic potential of probiotics is still controversial. probiotics could affect biofilm formation and interfere with adherence, growth or coaggregation with streptococcus mutans in biofilms. however, most of the studies have been conducted using planktonic bacteria. hence, the aim of the study was to assess the effect of probiotic bacteria on the cariogenicity of s. ... | 2015 | 26451810 |
| probiotics for prevention and treatment of diarrhea. | probiotics are increasingly used for prevention and treatment of diarrhea more in children than in adults. given the broad spectrum of diarrhea, this review focuses on the main etiologies: acute gastroenteritis, antibiotic-associated diarrhea (aad), and necrotizing enterocolitis (nec). for each, we reviewed randomized controlled trials, meta-analyses, and guidelines. for acute gastroenteritis we found 12 guidelines: 5 recommended probiotics and 7 did not. however, the guidelines containing posit ... | 2015 | 26447963 |
| lactobacillus rhamnosus cncmi-4317 modulates fiaf/angptl4 in intestinal epithelial cells and circulating level in mice. | identification of new targets for metabolic diseases treatment or prevention is required. in this context, fiaf/angptl4 appears as a crucial regulator of energy homeostasis. lactobacilli are often considered to display beneficial effect for their hosts, acting on different regulatory pathways. the aim of the present work was to study the effect of several lactobacilli strains on fiaf gene expression in human intestinal epithelial cells (iecs) and on mice tissues to decipher the underlying mechan ... | 2015 | 26439630 |
| live and heat-killed lactobacillus rhamnosus atcc 7469 may induce modulatory cytokines profiles on macrophages raw 264.7. | this study aimed to evaluate the capacity of lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (tnf-α, il-1β, il-4, il-6, il-10, and il-12) by mouse macrophages (raw 264.7). three microorganism preparations were used: live l. rhamnosus (llr) suspension, heat-killed l. rhamnosus (hklr) suspension, and the supernatant of a heat-killed l. rhamnosus (shklr) suspension, which were cultured with macrophages (37°c, 5% co2) for 2 h and 30 min. after that, cells were cultur ... | 2015 | 26649329 |
| relative cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic lactobacillus rhamnosus gg in managing infants with cow's milk allergy in spain. | to estimate the cost-effectiveness of using an extensively hydrolyzed casein formula containing the probiotic lactobacillus rhamnosus gg (ehcf + lgg; nutramigen lgg) as a first-line management for cow's milk allergy compared with ehcf alone, and amino acid formulae in spain, from the perspective of the spanish national health service (sns). | 2015 | 26648744 |
| efficient magnesium lactate production with in situ product removal by crystallization. | in this paper, attempts were made to develop an in situ product removal process for magnesium lactate production based on crystallization. the crystallization was conducted at 42°c without seed crystal addition. the product concentration, productivity and yield of fermentation coupled with in situ product removal (ispr) reached 143 g l(-1), 2.41 g l(-1)h(-1) and 94.3%. in four cycles of crystallization, the average reuse rate of fermentation medium and removal rate of product reached 64.0% and 7 ... | 2015 | 26433791 |
| short-chain fatty acids produced by synbiotic mixtures in skim milk differentially regulate proliferation and cytokine production in peripheral blood mononuclear cells. | short chain fatty acids (scfas) are major products of prebiotic fermentation and confer human health benefits such as immune-regulation. in this study, reconstituted skim milk supplemented with prebiotics (rsmp) including inulin, hi-maize or β-glucan was fermented by probiotic strains of lactobacillus spp. and bifidobacteria spp. after 24 h of fermentation, probiotics growth and scfas production were investigated and the produced scfas were extracted. inulin and lactobacillus rhamnosus gg atcc 5 ... | 2015 | 26398897 |
| feeding probiotic lactobacillus rhamnosus (mtcc 5897) fermented milk to suckling mothers alleviates ovalbumin-induced allergic sensitisation in mice offspring. | the neonatal period is often polarised to t helper (th2) response at the time of birth, predisposing offspring to allergic disorders. passive immunity through the mother's milk is critical for immune system development of newborns. probiotics have been proposed to harmonise th1/th2 imbalance in allergic conditions in adults. in the present study, the anti-allergic effects of feeding probiotic lactobacillus rhamnosus-fermented milk (pfm) either to dams during the suckling period or to their offsp ... | 2015 | 26330132 |
| influence of milk fermented with lactobacillus rhamnosus ncdc 17 alone and in combination with herbal ingredients on diet induced adiposity and related gene expression in c57bl/6j mice. | obesity has become a major health problem in developed countries and is rapidly catching up in the developing world due to changes in their life style. dietary incorporation of functional foods, including probiotic fermented milk and herbal ingredients, is being tried to ameliorate metabolic disorders. in the present study, the effect of dietary supplementation of a probiotic (lactobacillus rhamnosus ncdc 17) fermented milk alone or either of the herbal preparations (aloe vera/gymnema sylvestre ... | 2015 | 26327356 |
| effect of live and inactivated lactobacillus rhamnosus gg on experimentally induced rhinovirus colds: randomised, double blind, placebo-controlled pilot trial. | the aim of this work was to investigate the usability of an experimental rhinovirus model in probiotic trials aiming to assess effectiveness in viral infections, and to provide preliminary data of live and inactivated probiotic lactobacillus rhamnosus gg for larger-scale trials utilising the model. 59 subjects were randomised to receive 100 ml of fruit juice supplemented with 10(9) cfu of live or heat-inactivated (by spray-drying) l. rhamnosus gg or control juice daily for six weeks. after three ... | 2015 | 26322544 |
| antigenotoxic and antimutagenic activities of probiotic lactobacillus rhamnosus vc against n-methyl-n'-nitro-n-nitrosoguanidine. | the present study provides experimental evidence of in vivo reduction of genotoxic and mutagenic activities of potent carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng) by the strain lactobacillus rhamnosus vc. in vitro studies revealed that coincubation of mnng with viable cells of l. rhamnosus vc resulted in the detoxification of the parent compound accompanied with reduction in genotoxicity (69%) and mutagenicity (61%) as evaluated by sos-chromotest and ames test, respectively. oral feedi ... | 2015 | 26312410 |
| randomized controlled trial to determine the impact of probiotic administration on colonization with multidrug-resistant organisms in critically ill patients. | this was a randomized controlled pilot study of lactobacillus rhamnosus gg versus standard of care to prevent gastrointestinal multidrug-resistant organism colonization in intensive care unit patients. among 70 subjects, there were no significant differences in acquisition or loss of any multidrug-resistant organisms (p>.05) and no probiotic-associated adverse events. | 2015 | 26308946 |
| [lactobacillus rhamnosus gg inhibits cronobacter-induced meningitis in neonatal rats]. | to investigate the inhibitory effect of lactobacillus rhamnosus gg ( lgg) against cronobacter-induced meningitis in neonatal rats. | 2015 | 26277500 |
| effect of the probiotic lactobacillus rhamnosus on the expression of genes involved in european eel spermatogenesis. | positive effects of probiotics on fish reproduction have been reported in several species. in the present study, 40 male european eels were weekly treated with recombinant hcg for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) cfu/ml) of probiotic lactobacillus rhamnosus imc 501 (sinbyotec, italy). the probiotics were daily added to the water from the sixth week of the hcg treatment. males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of pr ... | 2015 | 26271165 |
| symbiotic maple saps minimize disruption of the mice intestinal microbiota after oral antibiotic administration. | this study was undertaken to evaluate the in vivo impact of new symbiotic products based on liquid maple sap or its concentrate. sap and concentrate, with or without inulin (2%), were inoculated with bifidobacterium lactis bb12 and lactobacillus rhamnosus gg valio at initial counts of 2-4 × 10(8) cfu ml(-1). the experiments started with intra-gastric administration of antibiotic (kanamycin 40 mg in 0.1 cc) (to induce microbiota disturbance and/or diarrhea) to 3-to-5-week-old c57bl/6 female mice ... | 2015 | 26218660 |
| impacts of ph-mediated eps structure on probiotic bacterial pili-whey proteins interactions. | probiotic bacteria are routinely incorporated into dairy foods because of the health benefits they can provide when consumed. in this work, the marked ph-dependence of the pili/eps organization at the outer surface of lactobacillus rhamnosus gg is characterized in detail by single cell force microscopy and cell electrophoretic mobility measurements analyzed according to formalisms for nanomechanical contact and soft particle electrokinetics, respectively. at ph 6.8, lgg pili are easily accessibl ... | 2015 | 26209966 |
| characterization of an antibacterial compound, 2-hydroxyl indole-3-propanamide, produced by lactic acid bacteria isolated from fermented batter. | lactic acid bacteria are known to produce numerous antimicrobial compounds that are active against various pathogens. here, we have purified and characterized a novel low-molecular-weight (lmw) antimicrobial compound produced by lactobacillus and pediococcus isolated from fermented idly and uttapam batter. the lmw compound was extracted from cell-free supernatant using ice-cold acetone, purified by gel permeation and hydrophobic interaction chromatography. it exhibited antimicrobial activity aga ... | 2015 | 26201479 |
| wheat straw degradation and production of alternative substrates for nitrogenase of rhodobacter sphaeroides. | cellulose is a major component of plant biomass and could be applied in the production of biofuels, especially bioethanol. an alternative approach is production of a clean fuel - hydrogen from cellulosic biomass. in this paper an innovatory model of cellulosic waste degradation has been proposed to verify the possibility of utilization of cellulose derivatives by purple non-sulfur bacteria. the concept is based on a two-step process of wheat straw conversion by bacteria in order to obtain an org ... | 2015 | 26192769 |
| pilins in gram-positive bacteria: a structural perspective. | pilins or fimbrilins are a class of proteins found in bacterial surface pilus, a hair-like surface appendage. both the gram-negative and -positive bacteria produce pilins to assemble pili on their cell-surface for different purposes including adherence, twitching motility, conjugation, immunomodulation, biofilm formation, and electron transfer. immunogenic properties of the pilins make them attractive vaccine candidates. the polymerized pilins play a key role in the initiation of host adhesion, ... | 2015 | 26178080 |
| inhibitory effect of lactobacillus salivarius on streptococcus mutans biofilm formation. | dental caries arises from an imbalance of metabolic activities in dental biofilms developed primarily by streptococcus mutans. this study was conducted to isolate potential oral probiotics with antagonistic activities against s. mutans biofilm formation from lactobacillus salivarius, frequently found in human saliva. we analysed 64 l. salivarius strains and found that two, k35 and k43, significantly inhibited s. mutans biofilm formation with inhibitory activities more pronounced than those of la ... | 2015 | 24961744 |
| endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection. | biotinylation is widely used in dna, rna and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. during the streptavidin-based detection of glycoproteins in lactobacillus rhamnosus gg with biotinylated lectin probes, a strong positive band of approximately 125 kda was observed, present in different cellular fractions. this potential glycoprotein reacted heavily with concanavalin a (cona), a lectin that specifically binds glucose and mannos ... | 2015 | 25211245 |
| perinatal outcomes of prenatal probiotic and prebiotic administration: an integrative review. | the purpose of this integrative review was to identify, critique, and synthesize the maternal and neonatal evidence on the prenatal use of probiotics and prebiotics to inform perinatal health professionals. a comprehensive literature search resulted in 37 studies of prenatal probiotics and 1 on antepartal prebiotics published from 1990 through 2011 that reported maternal, fetal, and/or neonatal outcomes. the methodologic quality of the studies reviewed was high, although investigators used diffe ... | 2015 | 24164813 |
| the mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria. | reconstituted skim milk (rsm) is a reputed protective carrier for improving the survival ratio of lactic acid bacteria (lab) after spray drying; however the underlying mechanisms of the prominent protection remains unclear. in this study, the inactivation histories of two lab strains during droplet drying with four carriers were experimentally determined, and the effects of droplet drying parameters on lab inactivation were investigated. for the first time, the possible contribution of each rsm ... | 2015 | 28455028 |
| probiotics for the prevention of pediatric antibiotic-associated diarrhea. | antibiotics are frequently prescribed in children. they alter the microbial balance within the gastrointestinal tract, commonly resulting in antibiotic-associated diarrhea (aad). probiotics may prevent aad via restoration of the gut microflora. | 2015 | 26695080 |
| probiotic attributes, antioxidant, anti-inflammatory and neuromodulatory effects of enterococcus faecium cfr 3003: in vitro and in vivo evidence. | accumulating evidence suggests that probiotic bacteria play a vital role in modulating various aspects integral to the health and well-being of humans. in the present study, probiotic attributes and the antioxidant, anti-inflammatory and neuromodulatory potential of enterococcus faecium cfr 3003 were investigated by employing suitable model systems. e. faecium exhibited robust resistance to gastrointestinal stress conditions as it could withstand acid stress at ph 1.5, 2 and 3. the bacterium als ... | 2015 | 26450608 |
| potentially probiotic and bioprotective lactic acid bacteria starter cultures antagonise the listeria monocytogenes adhesion to ht29 colonocyte-like cells. | the capability of five lactic acid bacteria (lab) to counteract the adhesion of listeria monocytogenes to the epithelial intestinal cell line ht29 was studied. the highest adhesion ability to ht29 was achieved by the intestinal strain lactobacillus rhamnosus ctc1679, followed by the meat-derived strains lactobacillus sakei ctc494 and enterococcus faecium ctc8005. surprisingly, the meat strains showed significantly better adhesion to ht29 than two faecal isolates of lactobacillus casei and even s ... | 2015 | 25488261 |
| an in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (mta) against five oral bacteria. | the purpose of this study was to evaluate the antibacterial ability of three mta (mta-angelus, endocem mta, and proroot mta) against five typical oral bacteria (streptococcus mutans, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus paracasei, and porphyromonas gingivalis). | 2015 | 26263538 |
| probiotic features of lactobacillus strains isolated from ragusano and pecorino siciliano cheeses. | in the present study 177 lactobacillus spp. strains, isolated from ragusano and pecorino siciliano cheeses, were in vitro screened for probiotic traits, and their characteristics were compared to those of lactobacillus rhamnosus gg, commercial strain. based on acidic and bile salt resistance, thirteen lactobacillus strains were selected. the multiplex-pcr application revealed that nine strains belonged to l. rhamnosus species and four to lactobacillus paracasei species. all selected strains were ... | 2015 | 25998823 |
| biodiversity and γ-aminobutyric acid production by lactic acid bacteria isolated from traditional alpine raw cow's milk cheeses. | "nostrano-cheeses" are traditional alpine cheeses made from raw cow's milk in trentino-alto adige, italy. this study identified lactic acid bacteria (lab) developing during maturation of "nostrano-cheeses" and evaluated their potential to produce γ-aminobutyric acid (gaba), an immunologically active compound and neurotransmitter. cheese samples were collected on six cheese-making days, in three dairy factories located in different areas of trentino and at different stages of cheese ripening (24 ... | 2015 | 25802859 |
| the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening. | the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ... | 2015 | 25475270 |
| deciphering meta-analytic results: a mini-review of probiotics for the prevention of paediatric antibiotic-associated diarrhoea and clostridium difficile infections. | meta-analyses are used to evaluate pooled effects of a wide variety of investigational agents, but the interpretation of the results into clinical practices may be difficult. this mini-review offers a three-step process to enable healthcare providers to decipher pooled meta-analysis estimates into results that are useful for therapeutic decisions. as an example of how meta-analyses should be interpreted, a recent meta-analysis of probiotics for the prevention of paediatric antibiotic-associated ... | 2015 | 24889895 |
| high protective efficacy of rice bran against human rotavirus diarrhea via enhancing probiotic growth, gut barrier function, and innate immunity. | previously, we showed that rice bran (rb) was able to reduce human rotavirus (hrv) diarrhea in gnotobiotic pigs. here, we investigated its effect on the growth of diarrhea-reducing probiotic lactobacillus rhamnosus gg (lgg) and escherichia coli nissle (ecn), and the resulting effects on hrv diarrhea, gut epithelial health, permeability and innate immune responses during virulent hrv challenge. on 3, 5, and 7 days of age pigs were inoculated with 2 × 10(4) colony-forming-units lgg+ecn to initiate ... | 2015 | 26459937 |
| op-3 the protective role of lactobacillus rhamnosus gg-derived factors against lps-induced damage of human colonic smooth muscle cells. | impaired gut barrier function has been reported in some functional gastrointestinal (gi) disorders.evidences suggest that gut microbiota affects gi motility in particular lactobacillus species elicits anti-inflammatory activity and exerts protective effects on damage induced by pathogen gram negative-derived lipopolysaccharide(lps).lps produced an oxidative imbalance in human colonic smooth muscle cells (smc) that persists after lps-washout and contributes to smc morphofunctional alterations. | 2015 | 26439544 |
| in vitro inhibition of 4-nitroquinoline-1-oxide genotoxicity by probiotic lactobacillus rhamnosus imc501. | inhibition of 4-nitroquinoline-1-oxide (4-nqo) genotoxicity by a probiotic strain of lactobacillus rhamnosus (imc501) was assessed by the prokaryotic short-term bioassay soschromotest, using escherichia coli pq37 as the target organism. results showed the ability of strain imc501 to rapidly and markedly counteract, in vitro, the dna damage originated by the considered genotoxin. the inhibition was associated with a spectroscopic hypsochromic shift of the original 4-nqo profile and progressive ab ... | 2015 | 26059518 |
| a selected lactobacillus rhamnosus strain promotes egfr-independent akt activation in an enterotoxigenic escherichia coli k88-infected ipec-j2 cell model. | enterotoxigenic escherichia coli (etec) are important intestinal pathogens that cause diarrhea in humans and animals. although probiotic bacteria may protect against etec-induced enteric infections, the underlying mechanisms are unknown. in this study, porcine intestinal epithelial j2 cells (ipec-j2) were pre-incubated with and without lactobacillus rhamnosus atcc 7469 and then exposed to f4+ etec. increases in tlr4 and nod2 mrna expression were observed at 3 h after f4+ etec challenge, but thes ... | 2015 | 25915861 |
| a tyrosine phosphorylation switch controls the interaction between the transmembrane modulator protein wzd and the tyrosine kinase wze of lactobacillus rhamnosus. | one proposed mechanism for assembly of secreted heteropolysaccharides by many gram positive bacteria relies on the coordinated action of a polymerization complex through reversible phosphorylation events. the role of the tyrosine protein kinase transmembrane modulator is, however, not well understood. | 2015 | 25885688 |
| effects of lactobacillus johnsonii and lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells. | heat shock proteins (hsps) are a set of highly conserved proteins that can serve as intestinal gate keepers in gut homeostasis. here, effects of a probiotic, lactobacillus rhamnosus gg (lgg), and two novel porcine isolates, lactobacillus johnsonii strain p47-hy and lactobacillus reuteri strain p43-huv, on cytoprotective hsp expression and gut barrier function, were investigated in a porcine ipec-j2 intestinal epithelial cell line model. the ipec-j2 cells polarized on a permeable filter exhibited ... | 2015 | 25847917 |
| characterization of lactobacillus isolated from dairy samples for probiotic properties. | in the present study twelve lactobacillus isolates (lbs 1-lbs 12) were characterized for probiotic properties. out of the twelve, eight isolates (lbs 1-6, 8 and 11) were bile resistant (survival > 50% at 0.3% bile salt w/v) and five isolates (lbs 1, 2, 5, 6 and 11) were found acid ph value resistant (survival > 50% at ph 3). all twelve isolates inhibited the growth of staphylococcus aureus whereas isolate lbs 2 also inhibited the growth of escherichia coli and salmonella typhimurium. antibiotic ... | 2015 | 25771244 |
| purification, crystallization and preliminary x-ray diffraction analysis of spad, a backbone-pilin subunit encoded by the fimbrial spafed operon in lactobacillus rhamnosus gg. | spad is the predicted backbone-pilin subunit of the spafed pilus, whose loci are encoded by the fimbrial spafed operon in lactobacillus rhamnosus gg, a gram-positive gut-adapted commensal strain with perceived probiotic benefits. in this study, soluble recombinant spad protein was overproduced in escherichia coli and then purified by ni2+-chelating affinity and gel-filtration chromatography. after limited proteolysis with α-chymotrypsin, good-quality crystals of spad were obtained which diffract ... | 2015 | 25615979 |
| in vitro characterization of the digestive stress response and immunomodulatory properties of microorganisms isolated from smear-ripened cheese. | thirty-six microorganisms (twenty-one bacteria, twelve yeasts and three fungi) were isolated from surface-ripened cheeses and subjected to in vitro digestive stress. the approach mimicked gastric and/or duodenal digestion. lactobacillus rhamnosus gg, escherichia coli nissle 1917 and saccharomyces boulardii were used as reference strains. we studied the microorganisms grown separately in culture medium and then included (or not) in a rennet gel. the microorganisms' immunomodulatory abilities were ... | 2015 | 25589362 |
| use of lactobacillus casei rhamnosus to prevent cholangitis in biliary atresia after kasai operation. | recurrent cholangitis may aggravate cholestatic liver cirrhosis in biliary atresia (ba) after the kasai operation. this pilot study aimed to investigate whether lactobacillus casei rhamnosus has the prophylactic efficacy for recurrent cholangitis in comparison with the conventional neomycin prophylaxis. | 2015 | 25534776 |
| gut commensal microvesicles reproduce parent bacterial signals to host immune and enteric nervous systems. | ingestion of a commensal bacteria, lactobacillus rhamnosus jb-1, has potent immunoregulatory effects, and changes nerve-dependent colon migrating motor complexes (mmcs), enteric nerve function, and behavior. how these alterations occur is unknown. jb-1 microvesicles (mvs) are enriched for heat shock protein components such as chaperonin 60 heat-shock protein isolated from escherichia coli (groel) and reproduce regulatory and neuronal effects in vitro and in vivo. ingested labeled mvs were detect ... | 2015 | 25392266 |
| lactobacillus rhamnosus gr-1 ameliorates escherichia coli-induced inflammation and cell damage via attenuation of asc-independent nlrp3 inflammasome activation. | escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. we explored the effects of the probiotic lactobacillus rhamnosus gr-1 on ameliorating e. coli-induced inflammation and cell damage in primary bovine mammary epithelial cells (bmecs). increased toll-like receptor 4 (tlr4), nod1, and nod2 mrna expression was observed following e. coli challenge, but this increase was attenuated by l. rhamnosus gr-1 pretreatment. immunofl ... | 2015 | 26655757 |
| antilisterial bacteriocin from lactobacillus rhamnosus cjnu 0519 presenting a narrow antimicrobial spectrum. | a lactic acid bacterium presenting antimicrobial activity against a lactobacillus acidophilus strain used for eradication of acid inhibition was isolated from a natural cheese. the 16s rrna gene sequence of the isolate best matched with a strain of l. rhamnosus and was designated l. rhamnosus cjnu 0519. the antimicrobial activity of the partially purified bacteriocin of cjnu 0519 was abolished when treated with a protease, indicating the protein nature of the bacteriocin. the partially purified ... | 2015 | 26761811 |
| lactic acid bacteria protect human intestinal epithelial cells from staphylococcus aureus and pseudomonas aeruginosa infections. | staphylococcus aureus and pseudomonas aeruginosa are opportunistic pathogens that cause nosocomial and food-borne infections. they promote intestinal diseases. gastrointestinal colonization by s. aureus and p. aeruginosa has rarely been researched. these organisms spread to extra gastrointestinal niches, resulting in increasingly progressive infections. lactic acid bacteria are gram-positive bacteria that produce lactic acid as the major end-product of carbohydrate fermentation. these bacteria i ... | 2015 | 26681052 |
| assessment of the in vitro antimicrobial activity of lactobacillus species for identifying new potential antibiotics. | the bacteriocin-mediated antimicrobial properties of lactobacillus spp. have been widely studied, leading to the use of these micro-organisms in the food industry as preservative agents against foodborne pathogens. in an era in which antibiotic resistance is becoming a public health issue, the antimicrobial activity of lactobacillus spp. could be used for the discovery of new potential antibiotics. thus, it is essential to have an accurate method of screening the production of antimicrobial agen ... | 2015 | 26163158 |
| can probiotic yogurt prevent diarrhoea in children on antibiotics? a double-blind, randomised, placebo-controlled study. | to estimate the efficacy of a probiotic yogurt compared to a pasteurised yogurt for the prevention of antibiotic-associated diarrhoea in children. | 2015 | 25588782 |
| lactobacillus rhamnosus cncm i-3690 and the commensal bacterium faecalibacterium prausnitzii a2-165 exhibit similar protective effects to induced barrier hyper-permeability in mice. | impaired gut barrier function has been reported in a wide range of diseases and syndromes and in some functional gastrointestinal disorders. in addition, there is increasing evidence that suggests the gut microbiota tightly regulates gut barrier function and recent studies demonstrate that probiotic bacteria can enhance barrier integrity. here, we aimed to investigate the effects of lactobacillus rhamnosus cncm i-3690 on intestinal barrier function. in vitro results using a caco-2 monolayer cell ... | 2015 | 25517879 |
| systematic review with meta-analysis: lactobacillus rhamnosus gg in the prevention of antibiotic-associated diarrhoea in children and adults. | the effects of probiotics are strain specific. the clinical effects of each strain need to be evaluated separately. | 2015 | 26365389 |
| the use of probiotics in pediatric gastroenterology: a review of the literature and recommendations by latin-american experts. | the stability and composition of intestinal flora plays a vital role in human wellbeing throughout life from as early as birth. over the past 50 years, several studies have been conducted to evaluate the effect of probiotic administration in pediatric gastroenterology. this document aims to provide a recommendation score on probiotic utilization in pediatric gastroenterology, together with a review of current knowledge concerning its benefits, tolerability, and safety. | 2015 | 25799959 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| probiotics reduce necrotizing enterocolitis severity in hiv-exposed premature infants. | to assess the effect of probiotics on the incidence of necrotizing enterocolitis (nec) in premature infants born to human immunodeficiency virus (hiv)-positive and hiv-negative women. | 2015 | 25724213 |
| [lactobacillus rhamnosus may change the virulence of candida albicans]. | to investigate the influence of lactobacillus rhamnosus in the expression of virulence factors of candida albicans in vitro. | 2015 | 26313880 |
| probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates. | this study investigates the antagonistic effects of the probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 against vulvovaginal candidiasis (vvc)-causing candida glabrata. | 2015 | 25688886 |
| inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions. | clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ... | 2015 | 25380800 |
| in vitro modulation of probiotic bacteria on the biofilm of candida glabrata. | a conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. in this st ... | 2015 | 26028405 |
| bugs and guts: practical applications of probiotics for gastrointestinal disorders in children. | probiotics are foods or products that contain live microorganisms that benefit the host when administered. in this clinical review, we evaluate the literature associated with using probiotics in common pediatric gastrointestinal disorders, focusing specifically on antibiotic-associated diarrhea, acute gastroenteritis, clostridium difficile infection (cdi), colic, inflammatory bowel disease, and functional gastrointestinal diseases. meta-analysis of several randomized controlled trials have confi ... | 2015 | 26538058 |
| probiotics: prevention of severe pneumonia and endotracheal colonization trial-prospect: protocol for a feasibility randomized pilot trial. | probiotics are defined as live microorganisms that may confer health benefits when ingested. meta-analysis of probiotic trials suggests a 25 % lower ventilator-associated pneumonia (vap) and 18 % lower infection rates overall when administered to patients in the intensive care unit (icu). however, prior trials are small, largely single center, and at high risk of bias. before a large rigorous trial is launched, testing whether probiotics confer benefit, harm, or have no impact, a pilot trial is ... | 2015 | 27965798 |
| lactobacillus species: taxonomic complexity and controversial susceptibilities. | the genus lactobacillus is a taxonomically complex and is composed of over 170 species that cannot be easily differentiated phenotypically and often require molecular identification. although they are part of the normal human gastrointestinal and vaginal flora, they can also be occasional human pathogens. they are extensively used in a variety of commercial products including probiotics. their antimicrobial susceptibilities are poorly defined in part because of their taxonomic complexity and are ... | 2015 | 25922408 |
| a decade of experience in primary prevention of clostridium difficile infection at a community hospital using the probiotic combination lactobacillus acidophilus cl1285, lactobacillus casei lbc80r, and lactobacillus rhamnosus clr2 (bio-k+). | in august 2003, the 284-bed community hospital pierre-le gardeur (plgh) in quebec experienced a major outbreak associated with the clostridium difficile nap1/027/bi strain. augmented standard preventive measures (spms) were not able to control this outbreak. it was decided in february 2004 to give to every adult inpatient on antibiotics, without any exclusion, a probiotic (bio-k+: lactobacillus acidophilus cl1285, lactobacillus casei lbc80r, and lactobacillus rhamnosus clr2) within 12 hours of t ... | 2015 | 25922400 |
| lactobacillus acidophilus cl1285, lactobacillus casei lbc80r, and lactobacillus rhamnosus clr2 (bio-k+): characterization, manufacture, mechanisms of action, and quality control of a specific probiotic combination for primary prevention of clostridium difficile infection. | a specific probiotic formulation composed of lactobacillus acidophilus cl1285, lactobacillus casei lbc80r, and lactobacillus rhamnosus clr2 (bio-k+) has been marketed in north america since 1996. the strains and the commercial products have been evaluated for safety, identity, gastrointestinal survival, and stability throughout shelf life. the capacity of both the fermented beverages and the capsules to reduce incidences of antibiotic-associated diarrhea and clostridium difficile infection (cdi) ... | 2015 | 25922399 |
| the effect of a multi-strain probiotic on the resistance toward escherichia coli challenge in a randomized, placebo-controlled, double-blind intervention study. | several probiotic strains have been shown to enhance human resistance to infectious disease. it is speculated that these strains may impose this effect by excretion of anti-microbial components, by competing with pathogens for intestinal nutrients and/or mucosal adhesion sites or modulating the immune system. | 2015 | 25369827 |
| microbiological characteristics of the probiotic strains b. longum bb536 and l. rhamnosus hn001 used in combination. | the aim of this study was to investigate the antagonistic activity between the probiotic strains bifidobacterium longum (b. longum) bb536 and lactobacillus rhamnosus (l. rhamnosus) hn001 (zircombi, alfa wassermann s.p.a., italy) and to evaluate for the strains tested alone and in combination the resistance in simulated gastrointestinal conditions, the ability to adhere to epithelial intestinal cells and their competition for adhesion. | 2015 | 26657925 |
| antagonistic characteristics against food-borne pathogenic bacteria of lactic acid bacteria and bifidobacteria isolated from feces of healthy thai infants. | food-borne pathogens are among the most significant problems in maintaining the health of people. many probiotics have been widely reported to alleviate and protect against gastrointestinal infections through antibacterial secretion. however, the majority of them cannot always play antagonistic roles under gut conditions. probiotic bacteria of human origin must possess other protective mechanisms to survive, out-compete intestinal flora and to successfully establish in their new host at a signif ... | 2015 | 26301060 |