Publications

current assays for hhv-8 seropositivity unreliable.		1998	9752829
dendritic cells derived from bone marrow and cd34+ selected blood progenitor cells of myeloma patients, cultured in serum-free media, do not contain the kaposi sarcoma herpesvirus genome.	the aim of our study was to test if dendritic cells contain the kshv genome. cd34+ peripheral blood progenitor cells (pbpc) and bone marrow mononuclear cells were cultured in x-vivo 15 medium supplemented with gm-csf and tnf-alpha in gas-permeable containers. dendritic cells were identified morphologically and immunophenotypically. the kshv genome was not identified in any of the cases using a nested primer pcr approach. serological analysis corroborated the molecular findings: no antibodies for ...	1998	9753067
squamous cell carcinomas after allogeneic bone marrow transplantation for aplastic anemia: further evidence of a multistep process.	secondary solid tumors are rare events occurring in patients who underwent allogeneic marrow transplantation for aplastic anemia and fanconi's anemia. human herpes virus 8 (hhv8), epstein-barr virus (ebv), and human papillomaviruses (hpv) sequences have been found in squamous cell carcinoma (scc) occurring in organ transplant recipients. the tumor suppressor gene p53 has been strongly linked to the occurrence of scc in the nonimmunocompromised population.	1998	9753353
suppression of hhv-8 viremia by foscarnet in an hiv-infected patient with kaposi's sarcoma and hhv-8 associated hemophagocytic syndrome.	human herpes virus 8 (hhv-8) seems to be involved in the pathogenesis of kaposi s sarcoma. in vitro, antiviral drugs with activity against herpes viruses also can suppress hhv-8, however, little is known about the antiviral activity against hhv-8 in vivo. in this report we describe the effects of foscarnet on hhv-8 viremia in an hiv-infected patient with disseminated kaposi s sarcoma and a presumably hhv-8 associated hemophagocytic syndrome. hhv-8 dna could be detected in this patient by pcr in ...	1998	9753702
no evidence of hhv-8 infection in patients with pemphigus vulgaris/foliaceus.		1998	9762040
molecular and serological examination of the relationship of human herpesvirus 8 to multiple myeloma: orf 26 sequences in bone marrow stroma are not restricted to myeloma patients and other regions of the genome are not detected.	human herpesvirus 8 (hhv-8) genomic sequences were recently detected by polymerase chain reaction (pcr) and in situ hybridization in bone marrow stromal cells grown from multiple myeloma (mm) patients, but not in cells from control subjects (rettig et al, science 276:1851, 1997). we sought to confirm these observations in our own group of mm patients (n = 30). dna was extracted from adherent stromal cells grown under varying conditions and assayed for hhv-8 sequence using pcr to amplify the orf ...	1998	9763550
dermatological findings correlated with cd4 lymphocyte counts in a prospective 3 year study of 1161 patients with human immunodeficiency virus disease predominantly acquired through intravenous drug abuse.	several prospective studies on dermatological findings in human immunodeficiency virus (hiv) type 1 infected patients have been published, mostly in populations in which the predominant risk factor for hiv infection is homosexuality. we attempted to identify cutaneous diseases associated with hiv-1 infection and to assess disease progression in a cohort of spanish patients in whom the predominant cause of hiv infection was intravenous drug abuse. we prospectively examined 1161 hiv-1-positive pat ...	1998	9764146
complete regression of aids-related kaposi's sarcoma-associated human herpesvirus-8 during therapy with indinavir.		1998	9764794
lymphoma cell lines: in vitro models for the study of hhv-8+ primary effusion lymphomas (body cavity-based lymphomas).	primary effusion lymphoma (pel; also known as body cavity-based lymphoma) is recognized as a new and unique lymphoma entity occurring predominantly, but not exclusively in human immunodeficiency virus (hiv)-seropositive patients with acquired immunodeficiency syndrome (aids). pel grows exclusively in body cavities as serous lymphomatous effusion without evidence of mass disease or dissemination. their most unique feature is infection with the newly discovered human herpesvirus-8 (hhv-8; also kno ...	1998	9766492
purification of kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) and analyses of the structural proteins.	the kaposis's sarcoma-associated herpesvirus (kshv) infected bcbl-1 cell line, adapted to and grown in medium containing 10% horse serum, was induced to lytic replication with 12-o-tetradecanoylphorbol-13-acetate (tpa) for virus production. supernatants from induced cells were filtered through a 0.45-microm filter and virions were concentrated by polyethylene glycol extraction and high speed centrifugation. the virus was purified by a glycerol gradient zonal centrifugation step followed by isopy ...	1998	9766893
progressive hhv-8-positive classic kaposi's sarcoma: rapid response to interferon alpha-2a but persistence of hhv-8 dna sequences in lesional skin.	the pathogenesis of kaposi's sarcoma (ks) is often attributed to an infectious agent. in particular, the human herpesvirus 8 (hhv-8) was currently shown to be closely related to all known ks types, including hiv-associated ks, european classic ks, african endemic ks and iatrogenic ks. we report here on an hiv-negative, german patient of neither jewish nor mediterranean descent with disseminated classic ks showing unusual rapid progression into the tumour stage. after systemic administration of i ...	1998	9767303
prevalence of human herpesvirus 8 antibodies in human immunodeficiency virus type 1-infected homosexual men in slovakia.		1998	9770082
bk virus as a potential co-factor in human cancer.	bk virus (bkv) is a member of the polyomavirus family that persistently infects 75-80% of the human population. bkv encodes a large t antigen which is responsible for the transforming functions of the virus. recent studies have shown an association of bkv dna with a variety of human tumours including pancreatic islet, brain, urinary tract and kaposi's sarcoma. despite the detection of bkv dna in several human tumours, there is no clear evidence for a causative role in tumour formation. we have s ...	1998	9776229
activation of human herpesvirus 8 (hhv-8) thymidine kinase (tk) tataa-less promoter by hhv-8 orf50 gene product is sp1 dependent.	human herpesvirus 8 (hhv-8) is a newly discovered virus closely associated with kaposi's sarcoma and primary effusion lymphomas. when they occur in patients with aids, these b-cell lymphomas frequently harbor another human herpesvirus, epstein-barr virus (ebv). to determine the molecular mechanisms of the regulation of early gene expression by the immediate-early gene products of hhv-8 and to assess possible molecular interactions between hhv-8 and ebv, we studied the regulation of the hhv-8 thy ...	1998	9778032
epidemic kaposi's sarcoma.	kaposi's sarcoma (ks) is the most common malignancy associated with human immunodeficiency virus-1 (hiv-1) infection and can result in significant morbidity. the clinical course of ks is quite variable, although for the majority of patients, ks is ultimately a progressive disease requiring systemic therapy. for early indolent ks, local therapies may be appropriate and may provide significant palliation. for patients with more advanced or rapidly progressive disease, systemic therapy is the treat ...	1998	9778686
human herpesvirus 8 and its role in the genesis of kaposi's sarcoma.		1998	9779358
comparison of the prevalence of antibodies to human herpesvirus 8 (kaposi's sarcoma-associated herpesvirus) in brazil and colorado.	the prevalence of human herpesvirus 8 (hhv-8; kaposi's sarcoma [ks] herpesvirus) infection was determined by ifa in 297 persons living in brazil and colorado. the prevalence of antibody to hhv-8 in human immunodeficiency virus (hiv) type 1-seropositive gay men with and without ks was similar in brazil and colorado. in brazil, the prevalence of hhv-8 antibody was significantly greater in hiv-1-seronegative gay men than in hiv-1-seronegative male intravenous drug users. hhv-8-seropositive brazilia ...	1998	9780272
study of human herpesvirus 8 (hhv-8) variants from kaposi's sarcoma in france: is hhv-8 subtype a responsible for more agressive tumors?		1998	9780286
kaposi's sarcoma-associated herpesvirus in kikuchi's disease.	kikuchi's disease is a fairly common self-limited disorder among orientals that usually involves the cervical lymph nodes of young individuals and occurs predominantly in females. frequently, the disease is associated with fever or flu-like symptoms, an elevated erythrocyte sedimentation rate (esr), neutropenia, and lymphocytosis with atypical lymphocytes in the peripheral blood, suggesting a viral origin. however, no infectious agent has been identified. the presence of kaposi sarcoma-associate ...	1998	9781647
infection in organ-transplant recipients.		1998	9786754
absence of a correlation between kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) and multiple myeloma.		1998	9787190
human herpesvirus-8 and relatives of patients with plasmocytic diseases.		1998	9787198
the chemokine kidnapping receptor of hhv8.		1998	9788957
chemokines activate kaposi's sarcoma-associated herpesvirus g protein-coupled receptor in mammalian cells in culture.	kaposi's sarcoma-associated herpesvirus (kshv)/human herpesvirus 8, a virus that appears to be involved in the pathogenesis of kaposi's sarcoma and primary effusion lymphomas, encodes a g protein-coupled receptor (kshv-gpcr) that exhibits constitutive signaling. in this report, we show that two chemokines, interleukin 8 (il-8) and growth-related protein-alpha, activate kshv-gpcr over constitutive levels. moreover, as with human receptors, the integrity of the elr motif of these chemokines is req ...	1998	9788958
[painless, red-violet skin lesions during immunosuppression].	a patient with painless, bluish-red skin lesions under immunosuppressive treatment for kidney transplantation is presented. the clinical and histological diagnosis was kaposi's sarcoma. this is a rare but serious complication in the immunocompromised patient. human herpes virus 8 (hhv 8) was recently found in the lesions from patients with kaposi's sarcoma. this supports the important role of immunosuppression in the development of this tumor. therapy consists of reduction or stopping of the imm ...	1998	9789478
[epidemiology of malignant non-hodgkin's lymphoma: current data].		1998	9789520
[castleman's disease of the abdomen].	plasmocytic variants of castleman's disease are uncommon. we report a new case of abdominal location with a rapidly fatal outcome. another particularity of that case was the negativity of kaposi's sarcoma associated herpesvirus, a virus recently implicated in human immunodeficiency virus associated castleman's disease.	1998	9791566
trauma-induced kaposi's sarcoma of the hallux. an unusual case.	kaposi's sarcoma is the most common malignant lesion in patients who test seropositive for the human immunodeficiency virus. although many cases of this tumor have been described in the literature, only a few cases have been related to koebner's phenomenon following trauma. biopsy of lesions remains the standard method of diagnosis, but the numerous treatment options available today require treatment to be determined on a case-by-case basis. the authors present an unusual case of trauma-induced, ...	1998	9791955
patterns of cytokine expression in aids-related non-hodgkin's lymphoma.	the pathogenesis of aids-related non-hodgkin's lymphomas (aids-nhl) involves accumulation of genetic lesions, stimulation and selection by antigen, as well as infection by viruses. deregulation of cytokine loops has also been proposed to contribute to aids-nhl development, although data are available only for a limited number of cytokines. in this study we have utilized a panel of aids-nhl cell lines to investigate in detail the pattern of tumour expression and production of a wide spectrum of c ...	1998	9792301
purified tat induces inflammatory response genes in kaposi's sarcoma cells.	kaposi's sarcoma (ks) is a neoplasm strongly associated with hiv-1 infection and marked by leukocytic infiltration. the infiltrating leukocytes are a possible source of inflammatory cytokines, human herpesvirus 8 (hhv8) and the hiv-1 transactivator protein tat. this study examines whether tat directly induces expression of cellular adhesion molecules and cytokines in ks cells and whether this induction differs in kinetics and magnitude from induction by tumour necrosis factor (tnf) alpha.	1998	9792375
increasing kaposi's sarcoma-associated herpesvirus seroprevalence with age in a highly kaposi's sarcoma endemic region, zambia in 1985.	kaposi's sarcoma (ks)-associated herpesvirus (kshv) is a newly discovered virus found in all forms of ks. in the united states, kshv infection appears to be most common amongst individuals at high-risk for ks. preliminary data from africa suggest that kshv infection may be much more common in the general population.	1998	9792393
oral manifestations of human immunodeficiency virus-infected patients in taiwan.	to understand the characteristic clinical features of human immunodeficiency virus (hiv)-related oral lesions and determine the prevalence of various oral lesions in hiv-infected patients in taiwan, we conducted a cross-sectional study of 207 hiv-infected patients at the taipei municipal institute for venereal disease control. overall, 108 (52.2%) patients had at least one oral lesion. the most common oral manifestation of hiv infection among these 207 patients was oral hairy leukoplakia (ohl, 2 ...	1998	9795527
antiviral activity of tumor-suppressor pathways: clues from molecular piracy by kshv.	a common feature of many tumor viruses is that they possess genes that produce specific proteins to inhibit major cellular tumor-suppressor pathways. despite intensive studies, the reasons why these diverse and unrelated viruses have independently evolved oncogenes remains obscure. kaposi-sarcoma-associated herpesvirus (kshv or hhv8) has pirated a number of recognizable cellular genes that are key to cell survival and proliferation. in this review, we provide an overview of the known activities ...	1998	9594662
pyothorax-associated lymphoma: relationship with epstein-barr virus, human herpes virus-8 and body cavity-based high grade lymphomas.	pyothorax-associated lymphoma (pal) is a newly-described entity developing several decades after artificial pneumothorax treatment for pulmonary or pleural tuberculosis. it is known to be associated with epstein-barr virus (ebv) with constant expression of the two latent membrane proteins: latent membrane protein (lmp)-1 and ebv-associated nuclear antigen (ebna)-2. we are reporting three new cases of pal. all of the tumours were of b-cell lineage and classified as large-cell diffuse lymphomas ac ...	1998	9596137
multiple myeloma and hhv8 infection.		1998	9596692
detection of human herpesvirus-8 dna in bone marrow biopsies from patients with multiple myeloma and waldenström's macroglobulinemia.		1998	9596693
abatement of cutaneous kaposi's sarcoma associated with cidofovir treatment.		1998	9597261
[kaposi sarcoma-associated herpesvirus infection of dendritic cells in bone marrow in patients with myelomatosis].		1998	9599525
herpes virus-like dna (hhv-8) in immunosuppressive therapy-related, hiv-related and classical kaposi's sarcoma in norwegian patients.	the recently discovered human herpes virus 8 (kaposi's sarcoma-associated herpes virus) has been implicated in the pathogenesis of kaposi's sarcoma. using polymerase chain reaction we detected dna sequences of this herpes virus in 11 of 14 biopsy specimens from kaposi's sarcoma in norwegian patients, including the immunosuppressive therapy-related type (3 of 3), the hiv-related type (4 of 5), and the classical type (4 of 6). the results support the hypothesis of a role for human herpes virus 8 i ...	1998	9602228
transient angiolymphoid hyperplasia and kaposi's sarcoma after primary infection with human herpesvirus 8 in a patient with human immunodeficiency virus infection.		1998	9603796
lack of serologic association between human herpesvirus-8 infection and multiple myeloma and monoclonal gammopathies of undetermined significance.		1998	9605649
distinguishing the states of latency and reactivation is important for human herpesvirus 8.		1998	9606337
high prevalence of antibodies to human herpesvirus 8 in relatives of patients with classic kaposi's sarcoma from sardinia.	a survey for antibodies to a recombinant small viral capsid antigen (svca) of human herpesvirus type 8 (hhv-8) was conducted in sardinia, one of the world's highest incidence areas for classic kaposi's sarcoma (ks). prevalence of antibodies to hhv-8 svca was greatest in patients with ks (95%), followed by family members (39%) and a sardinian control population age- and sex-matched to the relatives (11%). within families, prevalence of antibodies was about equal among spouses, children, and sibli ...	1998	9607855
[alpha interferons--new therapeutic modalities].	interferons are naturally occurring substances. in fact, interferons are intercellular signalling proteins produced by cells in response to various biological and synthetic stimuli. three major classes of interferons have been identified: interferons alpha, beta and gamma. interferons originate from natural sources and are products of recombinant technology. two forms of recombinant alpha-interferons, 2a and 2b, are available. alpha-interferons are secreted and synthetised by leucocytes and lymp ...	1998	9611956
human herpesvirus 8 in kaposi's sarcoma and kaposi's sarcoma-mimicking vascular tumors.	kaposi's sarcoma (ks) had been a rare and unusual vascular tumor until a recent epidemic of a disseminated and fulminant form of ks in aids patients. infectious agents have been suspected of causing ks, and recently partial genomic dna sequences of human herpesvirus 8 (hhv8) have been identified in aids-associated ks lesions. since then, genomic dna sequences of hhv8 have been isolated in other forms of ks. although the partial genomic dna sequence of hhv8 was reported to be, if rare, identified ...	1998	9539320
primary effusion lymphoma containing human herpesvirus 8 dna in two aids patients with kaposi's sarcoma.	primary effusion lymphomas (pels) containing kaposi's sarcoma-associated herpesvirus (human herpesvirus 8/hhv-8) dna sequences represent a distinct but heterogeneous group of rare non-hodgkin's lymphomas of null-cell phenotype/b-cell origin. we aimed to describe the clinicopathologic features of two human immunodeficiency virus (hiv)-related pels occurring in homosexual men with kaposi's sarcoma (ks).	1998	9542317
infection of primary dermal microvascular endothelial cells by kaposi's sarcoma-associated herpesvirus.	to develop an in vitro model for infection of primary human cells with kaposi's sarcoma (ks) herpesvirus (kshv).	1998	9543444
seroprevalence of human herpesvirus-8 in healthy subjects and patients with aids-associated and classical kaposi's sarcoma in france.		1998	9543459
high seroprevalence of human herpesvirus-8 in pregnant women and prostitutes from cameroon.		1998	9543461
effects of a urinary factor from women in early pregnancy on hiv-1, siv and associated disease.	the effects of clinical grade crude preparations of human chorionic gonadotropin (hcg) on kaposi's sarcoma, hiv, siv and hematopoiesis were examined in vitro and in vivo. in contrast to previous studies, we report that the antiviral activity of hcg associated factors is not due to the native hcg heterodimer, including its purified subunits or its major degradation product, the beta-core. using gel permeation chromatography of the clinical grade hcg and urine concentrates from pregnant women, we ...	1998	9546788
deregulation of cell growth by the k1 gene of kaposi's sarcoma-associated herpesvirus.	at a position equivalent to the gene encoding the saimiri transforming protein (stp) of herpesvirus saimiri (hvs), kaposi's sarcoma-associated herpesvirus (kshv) contains a distinct open reading frame called k1. although kshv and hvs are related members of the rhadinovirus subgroup of gamma herpesviruses, k1 and stp exhibit no similarity in amino acid sequence or in structural organization. since stp is required for the oncogenic potential of hvs, we investigated the functional consequence of k1 ...	1998	9546789
[new herpesviruses hhv 6, 7 and 8 and their clinical significance].		1998	9553303
consistent polymerase chain reaction-single-strand conformation polymorphism pattern of human herpesvirus-8 in the course of classical kaposi's sarcoma assumes its clonal origin.	there is emerging evidence that kaposi's sarcoma-associated herpesvirus (kshv or hhv-8) has a central role in the pathogenesis of kaposi's sarcoma (ks). the occurrence of hhv-8 in classical ks biopsies is reported irrespective of its clinical stage (patch, plaque, nodular). hhv-8 was detected in 25 of 28 formalin-fixed paraffin-embedded classical ks samples by nested polymerase chain reaction. in addition, in six patients multiple tumors were available (n = 21). single-strand conformation polymo ...	1998	9557296
the pathogenesis of kaposi's sarcoma.		1998	9795583
aids-related malignancies.		1998	9800109
kaposi's sarcoma herpesvirus as a new paradigm for virus-induced oncogenesis.	seroepidemiologic studies show that kaposi's sarcoma herpesvirus (kshv) is more prevalent in those populations at higher risk to develop kaposi's sarcoma (ks). anti-kshv antibody titer is also higher in those who develop ks. kshv is a sexually transmitted disease among homosexual men, but other routes of transmission appear to be more important in africa and mediterranean europe. kshv encodes numerous cellular homologues that might be involved in ks and lymphoma pathogenesis. the characterizatio ...	1998	9800110
emerging treatments for epidemic (aids-related) kaposi's sarcoma.	kaposi's sarcoma (ks) is an opportunistic tumor that develops with increased frequency (100,000-fold) after hiv infection. ks causes significant morbidity from mucocutaneous involvement and mortality from complications of visceral sites of disease such as the lungs, gastrointestinal tract, and the liver. progressive unraveling of the ks pathogenesis has lead to the development of novel therapeutic approaches. newest therapies are first evaluated in patients with limited tumor burden. these inclu ...	1998	9800111
transmission of human herpesvirus 8 infection from renal-transplant donors to recipients.	human herpesvirus 8 (hhv-8) has been detected in all forms of kaposi's sarcoma, including transplantation-associated kaposi's sarcoma. to investigate the possibility of transmission of hhv-8 through allografts, we measured the seroprevalence of hhv-8 before and after renal transplantation.	1998	9801396
human herpesvirus 8--let the transplantation physician beware.		1998	9801403
no evidence of human herpesvirus 8 infection in patients with paraneoplastic pemphigus, pemphigus vulgaris, or pemphigus foliaceus.	paraneoplastic pemphigus has been associated with both malignancies and multicentric castleman's disease; the latter is a rare angiolymphoproliferative disorder that has also been linked with human herpesvirus 8 (hhv8) infection. other diseases definitively associated with hhv8 include kaposi's sarcoma and primary effusion lymphoma. in a search for additional hhv8-associated diseases, patients with paraneoplastic pemphigus, as well as patients with pemphigus vulgaris and pemphigus foliaceus, wer ...	1998	9804338
topical and intralesional cidofovir: a review of pharmacology and therapeutic effects.	cidofovir is a potent nucleoside analog antiviral drug approved for the treatment of cytomegalovirus (cmv) retinitis in patients with aids. it is currently available only for intravenous infusion. several small studies and case reports describe the successful use of cidofovir applied either topically or intralesionally in several virally induced cutaneous diseases.	1998	9810890
immunoglobulin vh gene mutational analysis suggests that primary effusion lymphomas derive from different stages of b cell maturation.	primary effusion lymphoma (pel) is a recently described distinct subtype of non-hodgkin's lymphoma associated with infection by the kaposi's sarcoma-associated herpesvirus, also called human herpesvirus-8. most cases of pel are also associated with the epstein-barr virus (ebv). in order to better characterize the cellular origin of pel, we investigated the immunoglobulin (ig) heavy chain variable region (vh,) genes expressed by tumor cells of the bc-1 and bc-3 cell lines derived from pels and fi ...	1998	9811353
distinct biology of kaposi's sarcoma-associated herpesvirus from primary lesions and body cavity lymphomas.	the dna sequence for kaposi's sarcoma-associated herpesvirus was originally detected in kaposi's sarcoma biopsy specimens. since its discovery, it has been possible to detect virus in cell lines established from aids-associated body cavity-based b-cell lymphoma and to propagate virus from primary kaposi's sarcoma lesions in a human renal embryonic cell line, 293. in this study, we analyzed the infectivity of kaposi's sarcoma-associated herpesvirus produced from these two sources. viral isolates ...	1998	9811747
kaposi's sarcoma in a patient with erythroblastopenia and thymoma: reactivation after topical corticosteroids.	we report a 69-year-old female with erythroblastopenia and thymoma who developed lesions of kaposi's sarcoma (ks) after thymectomy, 2 months after the initiation of therapy with methylprednisolone. control of mucocutaneous ks lesions was obtained with radiotherapy, interferon alfa-2b and withdrawal of systemic immunosuppressive therapy. erosive oral lichen planus appeared later, and after therapy with topical corticosteroids a new lesion of ks developed that regressed after withdrawal of topical ...	1998	9812034
new advances in multiple myeloma.	recent studies have led to new insights into the pathogenesis of multiple myeloma, including appreciation of the potential role of human herpesvirus-8 infection and further characterization of some of the cytogenetic abnormalities present in this disease. new strategies to optimize the use of allogeneic and autologous stem cell transplantation include the development of various immunotherapeutic techniques to enhance the antitumor effect, and, in the case of allogeneic transplantation, to reduce ...	1998	9814656
detection of high nerve growth factor serum levels in aids-related and -unrelated kaposi's sarcoma patients.	nerve growth factor (ngf) is an autocrine survival factor for memory b lymphocytes. as functional b-cell deregulation is present during hiv infection, ngf serum levels were measured in hiv-infected patients and compared with the patients' clinical features.	1998	9814871
detection of human herpesvirus 8 dna sequences in tissues and bodily fluids.	human herpesvirus 8 (hhv-8) has been proposed as a sexually transmitted etiologic agent of kaposi's sarcoma (ks). in this study, by use of a sensitive polymerase chain reaction assay, hhv-8 dna was detected in the skin lesions (92%), normal skin (23%), peripheral blood mononuclear cells (pbmc) (46%), plasma (7%), saliva (37%), and semen (12%) but not stool samples from ks patients. the average number of hhv-8 copies per microgram of positive target dna was 64, 000, 9000, 40, 33,000, and 300 for ...	1998	9815212
seroprevalence of human herpesvirus 8 among zambian women of childbearing age without kaposi's sarcoma (ks) and mother-child pairs with ks.	the seroprevalence of human herpesvirus 8 (hhv-8) among a group of zambian women of reproductive age and among mother-child pairs in which either one of them has kaposi's sarcoma (ks) was determined. a cross-sectional group of 378 pregnant women was randomly recruited into the study, and 183 (48.4%) had hhv-8 antibodies. among the human immunodeficiency virus (hiv)-1-infected women, 51.1% were hhv-8-seropositive, whereas of hiv-1-negative women, 47.3% were hhv-8-seropositive. in addition, 21 wom ...	1998	9815235
serologic and molecular evidence of human herpesvirus 8 activation in renal transplant recipients.	this study was designed to determine whether there is serologic or molecular evidence of human herpesvirus 8 (hhv-8) activation in renal transplant patients, an immunocompromised population at risk for development of kaposi's sarcoma. indirect immunofluorescence for detection of hhv-8 serum antibody and southern blot polymerase chain reaction (pcr) for detection of viral dna in whole blood were used. seroprevalence and geometric mean titer (gmt) were significantly increased in the transplant gro ...	1998	9815236
aids oncology.	new diagnostic approaches to primary central nervous system lymphoma have been developed that may make brain biopsy unnecessary in many instances. reduced-dose chemotherapy for non-hodgkin's lymphoma has been shown to be advantageous in a randomized controlled trial. new agents for the treatment of kaposi's sarcoma are available. adoptive cellular immunotherapy has been shown to be effective in the treatment and prevention of epstein-barr virus-associated b-cell lymphomas in bone marrow transpla ...	1998	17033362
kaposi sarcoma-associated herpesvirus/human herpesvirus 8, cytokines, growth factors and hiv in pathogenesis of kaposi's sarcoma.	epidemiological studies have strengthened the case for kaposi sarcoma-associated herpesvirus/human herpesvirus 8 being the long-sought kaposi sarcoma agent, but have also pointed to a role for other co-factors. like other tumour viruses, kaposi sarcoma-associated herpesvirus/human herpesvirus 8 establishes a latent (persistent) infection in kaposi sarcoma-spindle (tumorous) cells, but can also undergo lytic replication in these and other cell types. several latent and lytic viral genes may play ...	1998	17033372
dermatological infections in the immunocompromised host.	the data from the most recent studies concerning infection with human herpes virus 8 strongly support a pathogenic role for this virus in the tumoral hyperplasia which is a feature of kaposi's sarcoma. the results from the first studies of the sensitivity of human herpes virus 8 to antiviral drugs were also published last year. this review also concerns bacterial and mycobacterial infections showing unusual presentation, important trials in the prevention of infection with varicella zoster virus ...	1998	17033375
the epidemiology and pathogenesis of infections caused by the high numbered human herpesviruses in children: hhv-6, hhv-7 and hhv-8.	human herpesvirus 6 may be transmitted from saliva to infants, but other routes of transmission, such as organ and bone marrow transplantation, have also been reported. intrauterine infection has also been suggested. although the clinical symptoms during the primary infection in children are mild, human herpesvirus 6 may have neurotropic properties and be involved in the pathogenesis of febrile seizures in infants. the clinical symptoms caused by human herpesvirus 7 are not yet clear, but report ...	1999	17035788
infections in solid organ transplant recipients.	a disturbing trend in evolution for sometime has culminated in the fact that resistant gram-positive bacteria including enterococci and staphylococci have emerged as the leading pathogens at many transplant centers. recently published reports have highlighted the formidable challenge such antimicrobial-resistant microorganisms now pose in transplant recipients. studies published within the past year have documented the clinical relevance of human herpesvirus-6 after transplantation, as well as t ...	1999	17035801
revealing data on infectiousness of hiv.		1999	12492047
human herpesvirus 8: seroepidemiology among women and detection in the genital tract of seropositive women.	an indirect ifa to detect antibodies against latent nuclear antigens of human herpesvirus 8 (hhv-8) was used to determine the prevalence of hhv-8 antibodies in 169 women attending a sexually transmitted diseases clinic and a human immunodeficiency virus (hiv) clinic at a london hospital. nested polymerase chain reaction was used to detect hhv-8 dna in 93 blood samples and 89 cervical brush scrapes (cbs). another 96 cbs from women attending a colposcopy clinic were also analyzed. the overall sero ...	1999	9841845
increased human herpesvirus 8 seroprevalence in young homosexual men who have multiple sex contacts with different partners.	the objective of this study was to evaluate the behavioral risks that are associated with human herpesvirus 8 (hhv-8) infection in a cohort of young homosexual men. seventy-nine subjects (ages 22-33 years) who completed a questionnaire about their sexual and drug use behavior over the preceding year were recruited from the san francisco young men's health study. plasma samples were tested for anti-hhv-8 antibodies using an indirect ifa. thirty-eight subjects (48.1%) were infected with hhv-8. hhv ...	1999	9841846
appearance of human herpesvirus 8 on electron microscopy.		1999	9882218
identification and characterization of kaposi's sarcoma-associated herpesvirus k8.1 virion glycoprotein.	kaposi's sarcoma-associated herpesvirus (kshv) has been consistently identified in kaposi's sarcomas (ks), body cavity-based lymphomas (bcbl), and some forms of castleman's disease. previous serological tests with ks patient sera have detected lytic-cycle polypeptides from kshv-infected bcbl cells. we have found that these polypeptides are predominantly encoded by the k8.1 open reading frame, which is present in the same genomic position as virion envelope glycoproteins of other gammaherpesvirus ...	1999	9882339
characterization and cell cycle regulation of the major kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) latent genes and their promoter.	retinoblastoma tumor suppressor protein (prb) inhibition by tumor virus oncoproteins has been attributed to the need for these viruses to promote lytic viral nucleic acid synthesis by unscheduled entry into the s phase of the cell cycle. kaposi's sarcoma-associated herpesvirus (kshv or hhv8) encodes a functional cyclin (vcyc) which is expressed during latency and can direct phosphorylation of prb. we mapped the two major latent transcripts encoding vcyc, latent transcript 1 (lt1) and lt2, by cdn ...	1999	9882349
dynamics study on the anti-human immunodeficiency virus chemokine viral macrophage-inflammatory protein-ii (vmip-ii) reveals a fully monomeric protein.	encoded by kaposi's sarcoma-associated herpesvirus, viral macrophage-inflammatory protein-ii (vmip-ii) is unique among cc chemokines in that it has been shown to bind to the cxc chemokine receptor cxcr4 as well as to a variety of cc chemokine receptors. this unique binding ability allows vmip-ii to block infection by a wide range of human immunodeficiency virus type i (hiv-1) strains, but the structural and dynamic basis for this broad range of binding is not known. 15n t1, t2 and 15n[-hn] nucle ...	1999	9890927
genetic characterization of hhv-8/kshv-positive primary effusion lymphoma reveals frequent mutations of bcl6: implications for disease pathogenesis and histogenesis.	human herpesvirus-8/kaposi sarcoma-associated herpesvirus-positive primary effusion lymphoma (pel) is a recently identified b-cell non-hodgkin lymphoma category characterized by liquid growth in the serous body cavities. apart from viral infection, no genetic alteration is known to be associated with pel and no recurrent cytogenetic abnormality has been identified in these lymphomas. yet the consistent monoclonality of pel indicates that the disease is not solely a virus-driven proliferation. he ...	1999	9892104
antiretroviral therapy with protease inhibitors in human immunodeficiency virus type 1- and human herpesvirus 8-coinfected patients.	human herpesvirus 8 (hhv-8) is believed to play a role in the pathogenesis of kaposi's sarcoma (ks) and possibly in other proliferative disorders often associated with human immunodeficiency virus type 1 (hiv-1) infection. recent case reports have indicated resolution of ks and clearance of hhv-8 dna from peripheral blood mononuclear cells (pbmc) in hiv-1-infected subjects following highly effective antiretroviral therapy, including hiv-1 protease inhibitors (pi), suggesting a possible activity ...	1999	9892398
seroprevalence of human herpesvirus 8 in human immunodeficiency virus 1-positive and human immunodeficiency virus 1-negative populations in japan.	to determine the seroprevalence of human herpesvirus 8 (hhv8) among human immunodeficiency virus 1 (hiv-1)-positive (hiv-1+) and hiv-1-negative (hiv-1-) populations in japan, 276 hiv-1+ patients and 1,000 hiv-1- blood donors were enrolled in this study. antibodies against hhv8 latency-associated nuclear antigen (lana) were examined through indirect immunofluorescent assay by using a b-cell line that was infected latently with hhv8 (body cavity-based lymphoma 1). an hhv8- and epstein-barr virus-n ...	1999	9892401
agonists and inverse agonists for the herpesvirus 8-encoded constitutively active seven-transmembrane oncogene product, orf-74.	a number of cxc chemokines competed with similar, nanomolar affinity against 125i-interleukin-8 (il-8) binding to orf-74, a constitutively active seven-transmembrane receptor encoded by human herpesvirus 8. however, in competition against 125i-labeled growth-related oncogene (gro)-alpha, the orf-74 receptor was highly selective for gro peptides, with il-8 being 10,000-fold less potent. the constitutive stimulating activity of orf-74 on phosphatidylinositol turnover was not influenced by, for exa ...	1999	9873037
the new herpesviruses: emerging pathogens of dermatological interest.	to discuss the current knowledge of 3 recently discovered human herpesviruses (hhv-6, hhv-7, and hhv-8), and to provide a dermatological point of view.	1999	9923785
transfusion of the hiv-seropositive patient: immunomodulation, viral reactivation, and limiting exposure to ebv (hhv-4), cmv (hhv-5), and hhv-6, 7, and 8.		1999	9924760
human herpesvirus 8 dna load in leukocytes of human immunodeficiency virus-infected subjects: correlation with the presence of kaposi's sarcoma and response to anticytomegalovirus therapy.	specific human herpesvirus 8 (hhv-8) dna sequences were found in leukocytes of 12 of 29 (41.4%) aids subjects with kaposi's sarcoma (ks), whereas they were found in 4 of 43 (9.3%) aids subjects without ks (p = 0.003), although the peak hhv-8 dna load in pcr-positive subjects with ks (mean, 425 copies per 0.2 microgram of dna) did not significantly differ from the one found in pcr-positive patients without ks (mean, 218 copies). the use of intravenous ganciclovir or foscarnet therapy to treat cyt ...	1999	9925538
degradation of p27(kip) cdk inhibitor triggered by kaposi's sarcoma virus cyclin-cdk6 complex.	the kaposi's sarcoma-associated human herpesvirus 8 (kshv/hhv8) encodes a protein similar to cellular cyclins. this cyclin is most closely related to cellular d-type cyclins, but biochemically it behaves atypically in various respects. complexes formed between the viral cyclin and the cyclin-dependent kinase subunit, cdk6, can phosphorylate a wider range of substrates and are resistant to cdk inhibitory proteins. we show here that the kshv-cyclin-cdk6 complex phosphorylates p27(kip) on a c-termi ...	1999	9927424
modulation of p27(kip1) levels by the cyclin encoded by kaposi's sarcoma-associated herpesvirus.	dna tumour viruses have evolved a number of mechanisms by which they deregulate normal cellular growth control. we have recently described the properties of a cyclin encoded by human herpesvirus 8 (also known as kaposi's sarcoma-associated herpesvirus) which is able to resist the actions of p16(ink4a), p21(cip1) and p27(kip1) cdk inhibitors. here we investigate the mechanism involved in the subversion of a g1 blockade imposed by overexpression of p27(kip1). we demonstrate that binding of k cycli ...	1999	9927425
antineoplastic urinary protein inhibits kaposi's sarcoma and angiogenesis in vitro and in vivo.	kaposi's sarcoma (ks) is the most common tumor in human immunodeficiency virus infection and acquired immune deficiency syndrome. recent clinical trials with human chorionic gonadotropin (hcg) prepared from early pregnancy urine have shown encouraging results in the resolution of ks lesions. a urinary protein with antitumor activity, anup (antineoplastic urinary protein), a dimer of 32 kd, has previously been shown to inhibit the growth of various tumor cell lines in vivo. it was thus studied fo ...	1999	9920854
[human herpesvirus 8 (hhv8). ii. pathogenic role and sensitivity to antiviral drugs].	human herpesvirus 8 (hhv8) has been found to be associated with three different diseases observed in aids patients: kaposi's sarcoma, primary effusion lymphoma, which is a rare type of non-hodgkin lymphomas affecting the body cavities, and multicentric castleman's disease. the role of this new herpesvirus and other lymphoid proliferations, like angioimmunoblastic lymphadenopathy or multiple myeloma, is much debatable. to date, there are several evidences for a direct role of this virus in the oc ...	1999	9920963
detection and sequence diversity of kaposi's sarcoma-associated herpesvirus (kshv)/human herpesvirus 8 (hhv-8) dna.	detection of kaposi's sarcoma (ks)-associated herpesvirus (kshv)/human herpesvirus (hhv-8) has been reported frequently in patients with ks associated with acquired immunodeficiency syndrome (aids). we examined the presence of the kshv sequence in 8 hiv-positive patients comprising 5 with ks, 2 with syphilis, 1 with prurigo, and 2 hiv-negative patients with angiosarcoma. using the polymerase chain reaction, we observed amplification of a dna fragment of the expected size in 4 patients with ks. s ...	1999	9920979
ifn-gamma induces endothelial cells to proliferate and to invade the extracellular matrix in response to the hiv-1 tat protein: implications for aids-kaposi's sarcoma pathogenesis.	previous studies indicated that the tat protein of hiv functions as a progression factor in kaposi's sarcoma (ks), an angioproliferative disease common and aggressive in hiv-1-infected individuals (aids-ks). in particular, tat that is released by infected cells stimulates the growth and invasion of spindle cells of endothelial origin derived from ks lesions (ks cells). other work suggested that inflammatory cytokines may act as initiating factors in ks since they induce normal endothelial cells ...	1999	9916748
big but weak: how many pathogenic genes does human herpesvirus-8 need to cause kaposi's sarcoma? (review).	human herpesvirus-8 (hhv-8) is implicated in the pathogenesis of kaposi's sarcoma (ks). in the last two years several hhv-8 gene products were heralded to 'cause ks' without any evidence that the respective gene is expressed in the tumor cells of ks lesions. in this framework it is of interest that almost 10% of the genes encoded by hhv-8 have been suggested to promote ks development due to either mitogenic, anti-apoptotic, chemoattractive, angiogenic or transforming activities. the rapidly grow ...	1999	9917504
usefulness of molecular detection of human herpesvirus-8 in the diagnosis of kaposi sarcoma by fine-needle aspiration.	kaposi sarcoma (ks), a multifocal angioproliferative disorder, occurs most commonly in patients with aids, in whom it remains an important cause of morbidity and mortality. ks is often in the differential diagnosis in hiv-infected patients undergoing fine-needle aspiration (fna). fna diagnosis of ks is usually made by morphologic observation of scant tissue fragments composed of bland spindle cells and crush artifact. human herpesvirus-8 (hhv-8) has been identified by polymerase chain reaction ( ...	1999	9894458
update on sarcoidosis.	the local inflammatory response in sarcoidosis appears to be a th1-mediated process. evidence exists that the systemic immune response, however, shows a th2 predominance. mycobacteria species, particularly cell wall-deficient forms, continue to be candidates for an infectious cause of the disease. evidence for a possible role for human herpesvirus 8 has also been submitted. the relationship between sarcoidosis and infectious pathogens remains open to investigation. both sjögren's syndrome and co ...	1999	9894636
human herpesvirus-8 infection in hemodialysis patients from northern italy.		1999	9893149
hydrocortisone activation of human herpesvirus 8 viral dna replication and gene expression in vitro.	patients undergoing chronic steroid therapy for organ transplantation are at increased risk for development of human herpes virus 8(hhv-8)-associated kaposi's sarcoma (ks). it has also been reported that following steroid withdrawal, ks lesions often undergo partial or complete regression.	1999	10096517
kaposi's sarcoma-associated herpesvirus: a sexually transmissible infection?	we examined sexual behavior as a risk factor for kaposi's sarcoma-associated herpesvirus (kshv) infection and examined the relation between kshv seropositivity and development of ks in cross-sectional and cohort studies of 130 homosexual men diagnosed with aids in sydney, australia during the period from 1991 to 1993. kshv serology was measured using antibody tests to latency-associated nuclear antigen (lana) and lytically expressed open reading frame (orf) 65.2. in the cross-sectional analysis, ...	1999	10096584