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cerebral infarct due to meningovascular neurobrucellosis: a case report.brucellosis is a common and multisystemic zoonotic infectious disease. central nervous system involvement is rarely seen in brucellosis, with an incidence of 0.5-25%. the aim of this report is to underline the importance of brucellosis, which is an endemic infection in our country, during the diagnostic evaluation of stroke.201019914117
hematologic manifestations of brucellosis in children: 5 years experience of an anatolian center.brucellosis continues to be an important cause of fever in underdeveloped countries and in the rural areas of developed world. it is a multisystemic disease, associated with a wide variety of symptoms. a wide variety of symptoms, including hematologic abnormalities, such as anemia, thrombocytopenia, pancytopenia, dissemine intravascular coagulation, and leucopenia could be seen. the aim of the study is to review the hematologic findings of brucellosis in childhood.201020147850
the changing etiology of fever of intermediate duration.fever of intermediate duration (fid) is a common condition. currently, its most frequent causes are not well defined.201020153558
application of variable number of tandem repeats typing to describe familial outbreaks of brucellosis in argentina.consumption of inadequately pasteurized dairy products is the most common means of transmission of brucellosis. this report describes two foodborne outbreaks that occurred in families infected after consumption of fresh home-made cheese bought in different argentine provinces. high resolution variable number of tandem repeats (vntr)-based analysis revealed two well-defined groups comprising essentially identical profiles and corresponding to the two different outbreaks. similar clinical findings ...201020167681
atp-binding cassette systems of brucella.brucellosis is a prevalent zoonotic disease and is endemic in the middle east, south america, and other areas of the world. in this study, complete inventories of putative functional abc systems of five brucella species have been compiled and compared. abc systems of brucella melitensis 16m, brucella abortus 9-941, brucella canis rm6/66, brucella suis 1330, and brucella ovis 63/290 were identified and aligned. high numbers of abc systems, particularly nutrient importers, were found in all brucel ...201020169092
the development of oral fluid-based diagnostics and applications in veterinary medicine.the purpose of this review was to discuss the history of the development and implementation of oral fluid diagnostics for infectious diseases of humans and domestic animals. the use of oral fluid for the assessment of health and diagnosis of disease in humans and animals has a surprisingly long history. as early as 1909, pollaci and ceraulo reported sensitive and specific agglutination of 'micrococcus melitensis' (brucella melitensis) by oral fluid from patients diagnosed with malta fever. a 198 ...201020202287
spatial distribution and risk factors of brucellosis in iberian wild ungulates.the role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. the aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several iberian wild ungulates.201020205703
travel-related cerebro-meningeal infections: the 8-year experience of a french infectious diseases unit.the etiological spectrum of cerebro-meningeal infections (cmi) in travelers has never been specifically analyzed.201020074095
cloning and expression of the immunoreactive brucella melitensis 28 kda outer-membrane protein (omp28) encoding gene and evaluation of the potential of omp28 for clinical diagnosis of brucellosis.brucellosis is a disease caused by gram-negative, facultative, intracellular bacteria belonging to the genus brucella. it is an emerging zoonosis, and an economically important infection of humans and livestock with a worldwide distribution. human infection is known to occur through consumption of infected raw milk, milk products and undercooked or raw meat. serodiagnosis of brucellosis is carried out by detection of antibodies generated against lps or whole-cell bacterial extracts by elisa or a ...201020075115
brucella melitensis infection in total knee arthroplasty: a case report.we report a case of a 63-year-old female patient who underwent a total knee arthroplasty in which the knee later became infected with brucella melitensis. diagnosis was made by positive culture of a sinus tract discharge. radiological views of the knee did not show signs of implant loosening. the patient was successfully treated with rifampicin and doxycycline without surgery.201020119673
recombinant omp28 antigen-based indirect elisa for serodiagnosis of bovine brucellosis.brucellosis is a zoonosis of both public health and economic importance in many developing countries including india. early detection and segregation of the infected animals are important in order to control the disease. serodiagnostic tests for brucellosis is mainly based on detection of antibodies developed against lipopolysaccharide (lps) component of cell. in this study we evaluated a protein antigen, 28 kda outer membrane protein (omp28), of brucella melitensis as an alternative to lps. rec ...201020025961
the type iv secretion system affects the expression of omp25/omp31 and the outer membrane properties of brucella melitensis.the type iv secretion system (t4ss) contributes to brucella intracellular survival through its effector proteins. comparative proteomic analysis showed that intracellular survival proteins are expressed differentially in a virb mutant. interestingly, several outer membrane proteins (omps) are also differentially expressed, implying that t4ss might affect the om properties of brucella. to further evaluate the impact of t4ss on om, in the present study, the om proteomes were isolated and compared. ...201020030728
protection of balb/c mice against brucella melitensis 16 m infection induced by vaccination with live escherchia coli expression brucella p39 protein.the periplasmic binding protein (p39) antigen of brucella melitensis 16 m was previously identified as th1 dominant antigens. in this study, the potential for this antigen to function as vaccine against b. melitensis 16 m infection in balb/c mice has been analyzed, and the humoral and cellular immune responses induced have been also characterized. mice were injected intraperitoneally with live escherichia coli alone or with that which express brucella p39, two times at 4 weeks intervals. the liv ...201020036752
protection of mice against brucella abortus 544 challenge by vaccination with recombinant omp28 adjuvanted with cpg oligonucleotides.brucella abortus, a gram negative, facultative intracellular pathogen causes brucellosis in many animal species and humans. although live, attenuated vaccines are available against this infection, they suffer from certain limitations. therefore, the development of an effective subunit vaccine against brucellosis is an area of intense research. the outer membrane proteins (omps) of brucella species have been extensively studied for its immunogenicity and protective ability. we have investigated t ...201020013309
evaluation of azithromycin, trovafloxacin and grepafloxacin as prophylaxis against experimental murine brucella melitensis infection.the prophylactic potential of the azalide azithromycin as well as the fluoroquinolones trovafloxacin and grepafloxacin was assessed for the control of infection with brucella melitensis in an experimental mouse model, determined by reduction in splenic bacterial burden. trovafloxacin showed limited protective efficacy when administered 2h following a low-dose b. melitensis challenge, whereas grepafloxacin was ineffective. in comparison, azithromycin provided significant control of infection both ...201020022474
brucellosis: a re-emerging zoonosis.brucellosis, especially caused by brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. the bacterial pathogen is classified by the cdc as a category (b) pathogen that has potential for development as a bio-weapon. brucella spp. are considered as the most common laboratory-acquired pathogens. the geographical distribution of brucellosis is constantly changing with new foci emerging or re-emerging. the disease occurs ...201019604656
brucellosis presenting as myelofibrosis: first case report.we describe the case of a 29-year-old woman who presented with pancytopenia and myelofibrosis. brucella melitensis was identified in her blood. the patient recovered completely with doxycycline and rifampin. a repeat bone marrow biopsy showed hypercellularity without myelofibrosis. bone marrow findings in cases of pancytopenia due to brucellosis reveal normocellularity, hypercellularity, hemophagocytosis, or granuloma. to our knowledge this is the first report of brucellosis causing myelofibrosi ...201019501533
genomic tandem repeat analysis proves laboratory-acquired brucellosis in veterinary (camel) diagnostic laboratory in the united arab emirates.we report a case of a 64-year-old veterinarian working in a state camel veterinary laboratory who was diagnosed with and treated for acute brucellosis with complicating epididymo-orchitis. genomic tandem repeat analysis (mlva-16) revealed identical brucella strains in patient cultures and from different dromedary milk samples positive for brucella melitensis, thereby confirming the diagnosis of a laboratory acquired infection. the case illustrates the high (airborne) infectivity of brucellosis i ...201019925579
evaluating the virulence of a brucella melitensis hemagglutinin gene in the caprine model.with the completion of the genomic sequence of brucella melitensis 16m, a putative hemagglutinin gene was identified which is present in 16m and absent in brucella abortus. the possibility of this hemagglutinin being a potential virulence factor was evaluated via gene replacement in b. melitensis yielding 16mδe and expression in trans in b. abortus 2308-qae. utilizing the caprine brucellosis model, colonization and pathogenesis studies were performed to evaluate these strains. b. melitensis 16m ...201020362205
attenuation of defined brucella melitensis wboa mutants.rough brucella mutants have been sought as vaccine candidates because they do not induce seroconversion. in this study, two defined nonreverting rough mutants were derived from virulent brucella melitensis strain 16m: a wboa deletion mutant designated wrr51 and a wboa purek dual deletion mutant designated wrrp1. strain wrrp1 exhibited reduced survival in human monocyte-derived macrophages (hmdms) compared with parent strain wrr51 or with δpurek strain wr201. strain wrrp1 persisted for 1 week or ...201020362622
risk factors for in-hospital mortality in infective endocarditis: five years' experience at a tertiary care hospital in turkey.despite recent improvements in diagnostic and therapeutic interventions, infective endocarditis (ie) is still associated with high in-hospital mortality rates. the study aim was to determine the clinical, laboratory and echocardiographic features of ie, and to evaluate the risk factors for in-hospital mortality.201020369506
global analysis of quorum sensing targets in the intracellular pathogen brucella melitensis 16 m.many pathogenic bacteria use a regulatory process termed quorum sensing (qs) to produce and detect small diffusible molecules to synchronize gene expression within a population. in gram-negative bacteria, the detection of, and response to, these molecules depends on transcriptional regulators belonging to the luxr family. such a system has been discovered in the intracellular pathogen brucella melitensis, a gram-negative bacterium responsible for brucellosis, a worldwide zoonosis that remains a ...201020387905
a 43-year-old woman with abdominal pain and fever. 201020440575
large scale immune profiling of infected humans and goats reveals differential recognition of brucella melitensis antigens.brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-lps antibodies. to better understand the universe of antibody responses that develop after b. melitensis infection, a protein microarray was fabricated containing 1,406 predicted b. melitensis proteins. the array was probed with sera from experimentally infected goats and naturally i ...201020454614
biochemical and functional analysis of tir domain containing protein from brucella melitensis.toll/interleukin-1 like receptors are evolutionarily conserved proteins in eukaryotes that play crucial role in pathogen recognition and innate immune responses. brucella are facultative intracellular bacterial pathogens causing brucellosis in animal and human hosts. brucella behave as a stealthy pathogen by evading the immune recognition or suppressing the tlr signaling cascades. brucella encode a tir domain containing protein, tcpb, which suppresses nf-kappab activation as well as pro-inflamma ...201020471373
brucella melitensis 16m produces a mannan and other extracellular matrix components typical of a biofilm.mutations in the brucella melitensis quorum-sensing (qs) system are involved in the formation of clumps containing an exopolysaccharide. here, we show that the overexpression of a gene called aiid in b. melitensis gives rise to a similar clumping phenotype. the aiid enzyme degrades ahl molecules and leads therefore to a qs-deficient strain. we demonstrated the presence of exopolysaccharide and dna, two classical components of extracellular matrices, in clumps produced by this strain. we also obs ...201020497223
brucella melitensis vjbr and c12-hsl regulons: contributions of the n-dodecanoyl homoserine lactone signaling molecule and luxr homologue vjbr to gene expression.quorum sensing is a communication system that regulates gene expression in response to population density and often regulates virulence determinants. deletion of the luxr homologue vjbr highly attenuates intracellular survival of brucella melitensis and has been interpreted to be an indication of a role for qs in brucella infection. confirmation for such a role was suggested, but not confirmed, by the demonstrated in vitro synthesis of an auto-inducer (ai) by brucella cultures. in an effort to f ...201020529360
epidemiological and phylogenetic analysis of spanish human brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpob typing.the severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. multiple-locus variable-number repeat analysis (mlva-16), hypervariable octameric oligonucleotide fingerprinting (hoof-print), and the differences in the single nucleotide polymorphisms (snps) (codons 1249 and 1309) of the dna-dependent rna polymerase beta subunit (rpob) were used to type a human brucella melitensis population (108 strain ...201020554816
evaluation of in vitro activities of tigecycline and various antibiotics against brucella spp.brucellosis is a zoonosis with a worldwide distribution and remains a significant public health problem mainly in the developing world. in this study we evaluated the in vitro activities and synergistic effects of antibiotic combinations against blood culture isolates of brucella spp. in vitro susceptibilities of 76 blood culture isolates of brucella melitensis and one blood culture isolate of brucella abortus to doxycycline, streptomycin, gentamicin, trimethoprim-sulfamethoxazole, moxifloxacin, ...201020568531
differences in the outer membrane-related properties of the six classical brucella species.outer membrane-related properties (such as auto-agglutination and susceptibility to various compounds) of strains representative of the six classical species of the genus brucella were assessed. the differences identified could not be fully explained based on the smooth or rough phenotype of the strain. smooth strains of the closely related species brucella melitensis and b. abortus exhibited different susceptibility patterns and the rough, virulent b. ovis and b. canis strains were equally or m ...201020576453
[spinal infections: typical and atypical imaging features].while the imaging features of established spondylodiscitis are well known, other presentations may be misleading. the purpose of this article is to review the typical imaging features of spondylodiscitis as well as some more atypical and unusual imaging features (early spondylodiscitis, sceptic spondylitis, septic arthritis of facet joints, primary epidural abscess, unusual germs, neonate).201020814395
acute acalculus cholecystitis and hepatitis caused by brucella melitensis.acute cholecystitis is a very rare presentation of brucellosis. a case of acalculous cholecystitis caused by brucella melitensis is reported with a review of previously reported cases.201020818096
mlva typing and antibiotic susceptibility of brucella human isolates from liaoning, china.we have characterized clinical isolates of brucella melitensis from liaoning, china, using multiple-locus variable-number repeat analysis of 16 loci (mlva-16) and evaluated the antimicrobial susceptibilities in vitro. all 31 isolates shared the panel 1 genotype 42 profile suggesting that they belong to the 'east mediterranean' group. in vitro antibiotic susceptibility was determined by the broth microdilution testing. all isolates were sensitive to tested antibiotics (rifampin, doxycycline, cipr ...201020832094
functional characterization of the incomplete phosphotransferase system (pts) of the intracellular pathogen brucella melitensis.in many bacteria, the phosphotransferase system (pts) is a key player in the regulation of the assimilation of alternative carbon sources notably through catabolic repression. the intracellular pathogens brucella spp. possess four pts proteins (eintr, npr, eiiantr and an eiia of the mannose family) but no pts permease suggesting that this pts might serve only regulatory functions.201020844759
coincidence of acute brucella hepatitis and dengue fever or serologic cross-reactivity?hepatic involvement in brucellosis is not uncommon since 10-20% of patient infected with brucella species can have abnormal liver function tests. the usual presentation of brucella hepatitis is in the form of chronic granulomatous hepatitis with mild to moderate elevation of liver enzymes, while acute hepatitis is rare. we report a young patient who presented with acute brucella-induced hepatitis and co-infection with dengue hemorrhagic virus resulting in severe elevation of liver enzymes and ab ...201020871199
selective amplification of brucella melitensis mrna from a mixed host-pathogen total rna.abstract:201020920187
identification of the essential brucella melitensis porin omp2b as a suppressor of bax-induced cell death in yeast in a genome-wide screening.inhibition of apoptosis is one of the mechanisms selected by numerous intracellular pathogenic bacteria to control their host cell. brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche.201020949000
genomic island 2 is an unstable genetic element contributing to brucella lipopolysaccharide spontaneous smooth-to-rough dissociation.brucella is a gram-negative bacterium that causes a worldwide-distributed zoonosis. the genus includes smooth (s) and rough (r) species that differ in the presence or absence, respectively, of the o-polysaccharide of lipopolysaccharide. in s brucellae, the o-polysaccharide is a critical diagnostic antigen and a virulence determinant. however, s brucellae spontaneously dissociate into r forms, a problem in antigen and s vaccine production. spontaneous r mutants of brucella abortus, brucella melit ...201020952568
the effect of tigecycline and ertapenem against clinical isolates of brucella melitensis detected by e-test on different media.in this study, in vitro activity of tigecycline (tig) and ertapenem (ert) against clinical isolates of brucella melitensis and the effect of different media on in vitro test results were investigated. the in vitro effects of tig and ert to 38 b. melitensis isolates were comparatively investigated in brucella agar and 5% sheep blood agar. mic value of ert was 0.032 μg/ml in 23 of 38 and 20 of 38 isolates on blood and brucella agar, respectively. minimum inhibitory concentration values of tig were ...201020966572
brucellosis infection presenting with cholestasis.brucellosis is a relatively common disease in the mediterranean area and may present with prolonged fever without focus, however it remains an important diagnostic challenge to most pediatricians. we report the case of a 10-year-old male patient who presented with fever without a focus of 10-day duration, hepatomegaly, ascites, a small elevation in transaminases and acute-phase reactants indicating cholestasis, leukopenia, and thrombocytopenia. imaging tests showed many small, rounded, hypodense ...201020579915
brucellosis in a patient with end-stage liver disease undergoing liver transplantation: successful treatment with tigecycline. 201020589653
in vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant brucella melitensis.brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. as an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. the aim of this study was to investigate the usage of native plants for brucellosis treatment. for this purpose, the anti-brucella activities of ethanolic and methanolic extracts o ...201020593515
disseminated intravascular coagulation in a case of brucellosis.brucellosis is a multisystem disease with a broad spectrum of clinical manifestations; hematological abnormalities ranging from fulminant as of disseminated intravascular coagulation (dic) to anaemia, leucopoenia, thrombocytopenia, and clotting disorders. in this report, we present dic in a case of brucellosis because of rare presentation. a 3-year-old boy was admitted with the complaints of continuous fever, vomiting, abdominal pain, and bruise on leg. he looked pale and his physical examinatio ...201020682596
diagnostic challenges of human brucellosis in hong kong: a case series in two regional hospitals.a retrospective analysis of six patients diagnosed with brucellosis in two regional hospitals was carried out. the epidemiological, clinical, and laboratory features were studied. all patients had exposure history. three patients presented with musculoskeletal symptoms, while three had predominantly genitourinary symptoms. one patient did not have fever at presentation. all patients were diagnosed by positive blood culture of brucella melitensis, and the diagnosis was not suspected for all excep ...201020683074
a rare case of seronegative neurobrucellosis.neurobrucellosis is one of the complications of brucellosis. we report a rare case of a 17-year-old girl with seronegative neurobrucellosis and depression and diplopia. results of agglutination tests for brucella both in serum and csf were negative. diagnosis was made only by positive culture of brucella mellitensis with inoculation of the patient's cerebrospinal fluid in a bactec 9050 system. the patient was successfully treated using ceftriaxone, doxycycline and rifampicin therapy for six mont ...201020697164
neurobrucellosis associated with syndrome of inappropriate antidiuretic hormone with resultant diabetes insipidus and hypothyroidism.neurological involvement of the central nervous system in brucellosis is uncommon. we describe a rare case of meningoencephalitis due to brucella melitensis infection, associated with the syndrome of inappropriate antidiuretic hormone secretion and leading to diabetes insipidus and hypothyroidism. neurobrucellosis, although rare, should be considered in cases of neurological disease of unknown etiology.201020702671
epidemiological characteristics of brucellosis in sheep and goats in bulgaria: 2005-2008.(full text is available at http://www.manu.edu.mk/prilozi). aim: to analyse and present the epidemiological and epizootological situation of brucellosis in humans, sheep and goats caused by brucella melitensis. methods: the veterinary health services analyzsd epidemiological reports, as well as other official documents, in relation to the epidemiology and the measures undertaken for control of brucellosis in bulgaria, 2005-08. results: in bulgaria animal infections caused by brucella melitensis ...201020703183
diagnostic validation of ovine and caprine brucellosis using serum- and milk-elisa.(full text is available at http://www.manu.edu.mk/prilozi). aim: to introduce and validate the new method of diagnosing ovine and caprine brucellosis in a rapid, accurate and inexpensive manner by using i-elisa (serum/milk) technique. methods: serum and milk samples from brucella rb and cft negative (n = 881) and positive (n = 755) animals were used. standardization of tests was through the bommeli elisa-besw (brucella bang) standard and our institute's (mkd) working standards (positive serum an ...201020703186
evaluation of the fluorescence polarization assay (fpa) for diagnosis of brucella melitensis infection of goats in argentina.(full text is available at http://www.manu.edu.mk/prilozi). aim: determine the optimal cut-off and the use of fluorescence polarization assay (fpa) to improve the detection of brucellosis in individual goats in argentina. methods: sera from 96 goats from a flock with abortion due to b. melitensis biovar 1 were used to assess the efficacy of the fpa to detect brucellosis in goats. fpa results were compared with those of the buffered antigen plate agglutination test (bpat) confirmed by seroaggluti ...201020703188
control and eradication strategies for brucella melitensis infection in sheep and goats.(full text is available at http://www.manu.edu.mk/prilozi). aim: to describe the various strategies for the control and eradication of b. melitensis infection in sheep and goats. methods: the advantages and drawbacks of these strategies are discussed on the basis of the author's personal experience and a revision of the relevant literature. results: vaccination programmes in various combinations can be applied either to decrease the prevalence of infection in the animal population or, when combi ...201020703189
brucellosis as priority public health challenge in south eastern european countries. 201020718078
serological, bacteriological, and molecular diagnosis of brucellosis in domestic animals in croatia.to present the surveillance data on brucella melitensis, b. suis, and b. ovis infection in cattle, sheep, goats, and swine in croatia obtained in 2008 by serological, bacteriological, and molecular methods for diagnostics of brucellosis in domestic animals.201020718085
immunization of mice with recombinant s-adenosyl-l-homocysteine hydrolase protein confers protection against brucella melitensis infection.due to drawbacks of live attenuated vaccines, much attention has been focused on screening brucella-protective antigens as subunit vaccine candidates. here, an immunoproteomic assay was used to identify the immunogenic soluble proteins of brucella melitensis 16m. in the present study, 27 unique immunogenic proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem ms (lc-ms/ms). from this set, the gene encoding one immunodominant protein ...201021166726
ovine and caprine brucellosis (brucella melitensis) in aborted animals in jordanian sheep and goat flocks.two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from september 2009 to april 2010 from the mafraq region of jordan. sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. one hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all brucella species in the collected samples which were obtained from 86 aborted animals (86/188 = ...201021052561
immunization of mice with gamma-irradiated brucella neotomae and its recombinant strains induces protection against virulent b. abortus, b. melitensis, and b. suis challenge.human brucellosis, a zoonotic disease of major public health concern in several developing countries, is primarily caused by brucella abortus, brucella melitensis, and brucella suis. no brucellosis vaccine is available for human use. the aim of this study was to determine if brucella neotomae, a bacterium not known to cause disease in any host, can be used for developing brucellosis vaccines. b. neotomae and its recombinant strains overexpressing superoxide dismutase and a 26 kda periplasmic pro ...201021109033
novel bioactive hydrophobic gentamicin carriers for the treatment of intracellular bacterial infections.gentamicin (gen) is an aminoglycoside antibiotic with a potent antibacterial activity against a wide variety of bacteria. however, its poor cellular penetration limits its use in the treatment of infections caused by intracellular pathogens. one potential strategy to overcome this problem is the use of particulate carriers that can target the intracellular sites of infection. in this study gen was ion-paired with the anionic aot surfactant to obtain a hydrophobic complex (gen-aot) that was formu ...201021115143
brucellosis reactivation after 28 years. 201021122256
morphological analysis of the sheathed flagellum of brucella melitensis.abstract:201021143933
identification of brucella by maldi-tof mass spectrometry. fast and reliable identification from agar plates and blood cultures.maldi-tof mass spectrometry (ms) is a reliable method for bacteria identification. some databases used for this purpose lack reference profiles for brucella species, which is still an important pathogen in wide areas around the world. we report the creation of profiles for maldi-tof biotyper 2.0 database (bruker daltonics, germany) and their usefulness for identifying brucellae from culture plates and blood cultures.201021151913
microangiopathic anemia of acute brucellosis - is it a true ttp?thrombotic thrombocytopenic purpura (ttp) is a severe disease, potentially fatal, if not diagnosed and treated promptly. ttp is clinically characterized by the pentad of thrombocytopenia, coombs-negative hemolytic anemia, fever, renal abnormalities and neurological disturbances. advances in recent years have delineated the molecular mechanisms of acquired and hereditary ttp.many infectious organisms have been reported to be associated with ttp, especially mycoplasma, but few cases of brucella in ...201021415981
splenic abscess due to brucella melitensis - a rare pediatric complication.splenic abscess due to brucella species is an extremely rare complication especially in acute illness. here we report a case of splenic abscess caused by brucella melitensis biotype 1 in a child with acute infection who was successfully treated with only antibiotics.201021346907
chronic, progressive back pain, fever and a noticeable paravertebral mass. 201121363970
imported brucellosis in denmark: molecular identification and multiple-locus variable number tandem repeat analysis (mlva) genotyping of the bacteria.a polymerase chain reaction was used to identify brucella species isolated from humans in denmark. consecutive analysis of referred bacteria and re-examination of historical isolates identified all as brucella melitensis. multiple-locus variable number tandem repeat analysis (mlva) placed the isolates in the previously defined 'east mediterranean' b. melitensis group.201121375425
brucellosis in pregnancy.this study was undertaken in order to evaluate the effect of brucellosis in pregnancy. the serum agglutination test, coombs and/or blood culture systems were used in the diagnosis of brucellosis. from july 2003 to september 2010, the clinical and delivery patterns of 40 healthy pregnant women were compared with 39 pregnant women who had brucellosis. there were no birth defects, anomalies or mortalities. we observed that brucellosis in pregnancy increases the incidence of preterm delivery (p = 0. ...201121378061
an analysis of children with brucellosis associated with pancytopenia.brucellosis produces a variety of nonspecific hematologic abnormalities. hematologic complications of mild anemia and leukopenia have been frequently associated with acute brucellosis, but pancytopenia is less frequently seen. in this study, records of children with brucellosis aged under or equal to 16 years, admitted to yuzuncu yil university hospital between 2004 and 2010, were analyzed retrospectively. over this time period, 187 patients with brucellosis were diagnosed. twenty-five (13.3%) o ...201121381870
association between brucella melitensis dna and brucella sp. antibodies. 201121411601
peculiarities of the regulation of the brucella flagellum. 201121415118
mlva genotyping of chinese human brucella melitensis biovar 1, 2 and 3 isolates.since 1950, brucella melitensis has been the predominant strain associated with human brucellosis in china. in this study we investigated the genotypic characteristics of b. melitensis isolates from china using a multiple-locus variable-number tandem-repeat analysis (mlva) and evaluated the utility of mlva with regards to epidemiological trace-back investigation.201122108057
[comparison of conventional methods and real-time multiplex polymerase chain reaction for identification and typing of brucella isolates of human origin].brucellosis which is a worldwide zoonotic disease, still constitutes a major public health problem in rural areas of turkey. the aim of the present study was to evaluate the species and biovar distribution of 187 presumptive brucella strains isolated from patients inhabiting at the provinces in eastern, south eastern and mediterranean regions over a 7-years period (from 2001 to 2007) and to compare multiplex real-time-polymerase chain reaction (m-rt-pcr) and conventional biotyping for the differ ...201121935772
brucella melitensis infection within warthin tumor of the parotid gland.brucellosis is a zoonotic systemic infectious disease, and multiorgan involvement is commonly seen, but involvement of the neck is a rare presentation of brucellosis. granulomatous infections of the parotid gland are extremely rare. warthin tumor is a well-known benign neoplasm of the salivary glands. in this report, we describe a warthin tumor associated with brucella melitensis in the same parotid gland.201121959459
limitations of the standard agglutination test for detecting patients with brucella melitensis bacteremia.abstract the sensitivity of the standard agglutination test (sat) for detecting brucellosis was determined in 264 israeli patients from whom a positive blood culture for brucella melitensis and serology were obtained within±1 week. a sat titer ≥1:160 had a diagnostic sensitivity of 91.7%, whereas raising the cutoff to ≥1:320, as recommended to decrease false-positive rates in endemic areas, reduced the sensitivity to 82.6%. physicians working in regions endemic for brucellosis should be aware ...201121919731
The Brucella TIR-like protein TcpB interacts with the death domain of MyD88.The pathogen Brucella melitensis secretes a Toll/interleukin-1 receptor (TIR) domain containing protein that abrogates host innate immune responses. In this study, we have characterized the biochemical interactions of Brucella TIR-like protein TcpB with host innate immune adaptor proteins. Using protein-fragment complementation assays based on Gaussia luciferase and green fluorescent protein, we find that TcpB interacts directly with MyD88 and that this interaction is significantly stronger than ...201122155231
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.Brucella melitensis is the etiological agent responsible for brucellosis. Present in the B. melitensis genome is a 116-residue protein related to arsenate reductases (Bm-YffB; BR0369). Arsenate reductases (ArsC) convert arsenate ion (H(2)AsO(4)(-)), a compound that is toxic to bacteria, to arsenite ion (AsO(2)(-)), a product that may be efficiently exported out of the cell. Consequently, Bm-YffB is a potential drug target because if arsenate reduction is the protein's major biological function t ...201121904062
modulation of microtubule dynamics by a tir domain protein from the intracellular pathogen, brucella melitensis.tir domain containing proteins play a crucial role in innate immunity in eukaryotes. brucella is a highly infectious intracellular bacterium that encodes a tir domain protein (tcpb) to subvert host innate immune responses to establish a beneficial niche for pathogenesis. tcpb inhibits nf-+¦b activation and pro-inflammatory cytokine secretions mediated by toll like receptor (tlr) 2 & 4. here, we demonstrate that tcpb modulates microtubule dynamics by acting as a stabilization factor. tcpb increas ...201121692747
isolation rates of brucella melitensis in an endemic area and implications for laboratory safety.a retrospective study was conducted to assess the potential threat posed by processing blood cultures to clinical microbiology laboratory personnel working in an area endemic for brucella melitensis in southern israel. the computerized laboratory records for the 2002-2009 period were reviewed, and the proportion of aerobic vials from which brucella organisms were isolated out of the total number of positive aerobic blood culture vials was determined. during the 8-year period, b. melitensis was i ...201121725863
epidemiological survey of rifampicin resistance in clinic isolates of brucella melitensis obtained from all regions of turkey.the aim of the present study was to assess the antimicrobial susceptibility of brucella melitensis isolates to rifampicin (rif) depending on time and regional differences. a total of 94 human brucella isolates collected in an 8-year period from the beginning of 2002 to the end of 2009 throughout turkey were investigated. the isolates were identified at species and biovar levels by conventional methods, and minimum inhibitory concentrations (mic) of rif was determined by using the e test method. ...201121826587
protective efficacy and safety of brucella melitensis 16m{delta}mucr against intraperitoneal and aerosol challenge in balb/c mice.brucellosis is a zoonosis of nearly worldwide distribution. vaccination against this pathogen is an important control strategy to prevent the disease. currently licensed vaccine strains used in animals are unacceptable for human use due to undesirable side effects and modest protection. substantial progress has been made during the past 10 years toward the development of improved vaccines for brucellosis. in part, this has been achieved by the identification and characterization of live attenuat ...201121708998
brucella melitensis infection following military duty in iraq.brucellosis is a common zoonotic disease worldwide; however, few cases are reported in the us. brucella melitensis infections are primarily acquired via consumption of high-risk foods or travel to endemic areas. we describe a case of b. melitensis infection in a tennessee soldier following deployment in iraq. initial symptoms included knee and back pain. culture of an aspirate of the left sacroiliac joint yielded b. melitensis. genetic analysis indicated that this isolate came from the middle ea ...201121824352
extended safety and efficacy studies of the attenuated brucella vaccine candidates 16m{delta}vjbr and s19{delta}vjbr in the immunocompromised irf-1-/- mouse model.the global distribution of brucellosis and high incidence in certain areas of the world warrants the development of a safer and efficacious vaccine. for the past ten years, we have focused our attention on the development of a safer, but still highly protective, live-attenuated vaccine for human and animal use. we have demonstrated the safety and protective efficacy of the vaccine candidates 16mδvjbr and s19δvjbr against homologous and heterologous challenge in multiple immunocompetent animal mo ...201122169089
structure of fructose bisphosphate aldolase from bartonella henselae bound to fructose 1,6-bisphosphate.fructose bisphosphate aldolase (fbpa) enzymes have been found in a broad range of eukaryotic and prokaryotic organisms. fbpa catalyses the cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. the ssgcid has reported several fbpa structures from pathogenic sources, including the bacterium brucella melitensis and the protozoan babesia bovis. bioinformatic analysis of the bartonella henselae genome revealed an fbpa homolog. the b. henselae fbpa enzym ...201121904049
Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine.In order to screen immunogenic candidate antigens for the development of a brucellosis subunit vaccine, an immunoproteomic assay was used to identify immunogenic proteins from Brucella melitensis 16 M soluble proteins. In this study, a total of 56 immunodominant proteins were identified from the two-dimensional electrophoresis immunoblot profiles by liquid chromatography tandem mass spectrometry (LC-MS/MS). Two proteins of interest, riboflavin synthase alpha chain (RS-a) and Loraine synthase (LS ...201121943783
BrabA.11339.a: anomalous diffraction and ligand binding guide towards the elucidation of the function of a 'putative ß-lactamase-like protein' from Brucella melitensis.The crystal structure of a ß-lactamase-like protein from Brucella melitensis was initially solved by SAD phasing from an in-house data set collected on a crystal soaked with iodide. A high-resolution data set was collected at a synchroton at the Se edge wavelength, which also provided an independent source of phasing using a small anomalous signal from metal ions in the active site. Comparisons of anomalous peak heights at various wavelengths allowed the identification of the active-site metal i ...201121904058
systems biology approach predicts antibody signature associated with brucella melitensis infection in humans.a complete understanding of the factors that determine selection of antigens recognized by the humoral immune response following infectious agent challenge is lacking. here we illustrate a systems biology approach to identify the antibody signature associated with brucella melitensis (bm) infection in humans and predict proteomic features of serodiagnostic antigens. by taking advantage of a full proteome microarray expressing previously cloned 1406 and newly cloned 1640 bm genes, we were able to ...201121863892
Brucella melitensis Survival During Manufacture of Ripened Goat Cheese at Two Temperatures.Abstract The aim of the current work was to assess the influence of two temperatures, 4°C and 24°C, on pH and water activity and their association with Brucella melitensis survival during the traditional manufacture of ripened goat cheese. Raw milk from a brucellosis-free goat herd was used for the manufacture of ripened cheese. The cheese was inoculated with 5×10(9) of the B. melitensis 16M strain during the tempering stage. The cheeses were matured for 5, 20, and 50 days at both temperatures ...201122129442
roles of genomic island 3 (gi-3) bab1_0278 and bab1_0263 open reading frames (orfs) in the virulence of brucella abortus in balb/c mice.the genomic island 3 (gi-3) shared by brucella melitensis and brucella abortus contains 29 genes encoding mostly unknown proteins. within this island, the open reading frames (orfs) bab1_0278 and bab1_0263 are present, bab1_0278 encodes a hypothetical protein of 64 amino acids sharing a domain with the gcra superfamily, whereas the amino acid sequence of bab1_0263 showed 42% identity with an iron regulated lsr2 protein. we obtained one deletion mutant for each one of these orfs present within th ...201122005180
lymphocyte activation test for diagnosis of seronegative brucellosis in humans.background: seronegative brucellosis is occasionally encountered in clinical practice especially in localized disease where diagnosis is reached mainly through positive blood culture. cellular immune responses are pivotal for protection against intracellular bacteria such as brucella. this study was performed to evaluate the expression of activation markers on peripheral blood mononuclear cells in response to in vitro stimulation by whole-cell suspension of brucella melitensis for the diagnosis ...201122234109
[spondylodiscitis: which etiology?].spondylodiskitis is a rare condition in adults but with high index of morbidity and mortality due to difficult diagnosis. the insidious evolution and the unspecificity of symptoms interfere with prompt treatment, being the diagnosis based primarily on a high index of suspicion. there are two major groups of spondylodiskitis: pyogenic, being staphylococcus aureus the most frequent etiological pathogen, and granulomatous, caused mainly by mycobacterium tuberculosis and brucella melitensis, both en ...201122713202
the first international standard anti-brucella melitensis serum.the world organisation for animal health (oie) requested an international standard anti-brucella melitensis serum (isabms) to standardise diagnostic tests and reagents for sheep and goats. the agreed criteria were the highest dilution (in negative serum) of the standard which must give a positive result and the lowest dilution (in negative serum) which must simultaneously give a negative result. the two dilutions for each assay were, respectively: indirect enzyme-linked immunosorbent assay (ieli ...201122435193
characterization of outer membrane vesicles from brucella melitensis and protection induced in mice.the outer membrane vesicles (omvs) from smooth b. melitensis 16 m and a derived rough mutant, vtrm1 strain, were purified and characterized with respect to protein content and induction of immune responses in mice. proteomic analysis showed 29 proteins present in omvs from b. melitensis 16 m; some of them are well-known brucella immunogens such as sod, groes, omp31, omp25, omp19, bp26, and omp16. omvs from a rough vtrm1 induced significantly higher expression of il-12, tnfα, and ifnγ genes in bo ...201122242036
deep-sequencing analysis of the mouse transcriptome response to infection with brucella melitensis strains of differing virulence.brucella melitensis is an important zoonotic pathogen that causes brucellosis, a disease that affects sheep, cattle and occasionally humans. b. melitensis strain m5-90, a live attenuated vaccine cultured from b. melitensis strain m28, has been used as an effective tool in the control of brucellosis in goats and sheep in china. however, the molecular changes leading to attenuated virulence and pathogenicity in b. melitensis remain poorly understood. in this study we employed the illumina genome a ...201122216095
brucellosis presenting as septic shock.brucellosis generally presents with fever, malaise, weight loss and bone pain with either an abrupt or insidious onset. a 76-year-old man presented in april 2010 with fever of 103°f, severe tachycardia, tachypnoea and a blood pressure of 80/50 mm hg requiring fluids and vasopressor support with norepinephrine. the patient had brucellosis in 1956 which was treated for 'many weeks' with tetracycline and streptomycin. he has had no recurrences since that time. he denies recent travel outside the us ...201122701076
brucella arthritis of the knee, 1 year after revision of anterior cruciate ligament reconstruction.brucellosis is a zoonotic infection with a broad spectrum of clinical manifestations. the authors report the first case in the literature of septic arthritis of the knee 1 year after revision of anterior cruciate ligament reconstruction. brucella melitensis biotype 3 was found in both synovial fluid and blood cultures. the patient was treated initially with arthroscopic debridement. after the diagnosis was confirmed, a second arthroscopic lavage and metal work removal was applied leaving the gra ...201122700607
extrapulmonary tuberculosis: importance of differential diagnosis.patients with tuberculosis (tb) typically present with pulmonary tb, but extrapulmonary tb is also an important clinical problem. in particular, osteoarticular tb has a great morbidity and its diagnosis gold-standard includes culture and histopathology of the infected area. the present case study describes a 53-year-old female portuguese farmer who had initial clinical and imaging features suggestive of tuberculous osteomyelitis. however, she denied any tb contacts and the tuberculin skin-test a ...201122679255
[pet-ct scan in a case of suspicion of metastatic dissemination due to the presence of multiple pulmonary nodules]. 201121440334
expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of brucella melitensis m5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats.antibodies produced in animals vaccinated using live attenuated vaccines against brucella spp. are indistinguishable using current conventional serological tests from those produced in infected animals. one potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy. corresponding methods of detection for antibodies raised by the marker vaccine should also be developed. a specific frag ...201121446957
antimicrobial susceptibilities of brucella isolates from various clinical specimens.brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. in the study we claimed to identify brucella species from clinical samples of patients with active brucellosis from van region of eastern anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-brucella agents and a possible new alternative tigecycline.201121448305
brucellosis presenting as piriformis myositis: a case report. 201121450058
isolation of brucella melitensis from a rb51-vaccinated seronegative goat.the purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis and vaccinated with rb51 located in mexico. the serological diagnosis of brucellosis in 33 animals was performed. the study included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and vaginal exudate were subjected to bacteriological study. all animals were negative for serological diagnosis, and isolation of ...201121455694
bilateral pulmonary nodules due to brucellosis. 201121470756
complete genome sequences of brucella melitensis strains m28 and m5-90, with different virulence backgrounds.brucella melitensis is a gram-negative coccobacillus bacteria belonging to the alphaproteobacteria subclass. it is an important zoonotic pathogen that causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. the b. melitensis strain m5-90, a live attenuated vaccine cultured from the b. melitensis virulent strain m28, has been an effective tool to control brucellosis in goats and sheep in china. here we report the complete genome sequences of b. melitensis m28 and m5-90, strai ...201121478357
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