Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| serum factors and v-src control two complementary mitogenic pathways in quail neuroretinal cells in culture. | quail neuroretinal cells (qnr cells) from 7-day-old embryos do not proliferate even in the presence of 8% fetal calf serum. after infection by the rous sarcoma virus (rsv) they proliferate actively and exhibit a transformed phenotype; this effect is mediated by the oncoprotein pp60v-src. secondary cultures infected by the thermosensitive strain tsny68 of rsv are blocked in g0 either by thermal inactivation of pp60v-src at 41.5 degrees c or by serum deprivation at the permissive temperature (36.5 ... | 1993 | 8437841 |
| bovine respiratory syncytial virus nucleocapsid protein expressed in insect cells specifically interacts with the phosphoprotein and the m2 protein. | the coding region of the gene for the nucleocapsid (n) protein of bovine respiratory syncytial virus has been inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using transfer vector pvl 1393. infection of spodoptera frugiperda cells with recombinant virus resulted in the synthesis of high levels of n protein. this protein was indistinguishable from the authentic bovine respiratory syncytial virus (brsv) n protein by sds-gel electrophoresis and immunoprecipitat ... | 1993 | 8438581 |
| a human respiratory syncytial virus (rsv) primate model of enhanced pulmonary pathology induced with a formalin-inactivated rsv vaccine but not a recombinant fg subunit vaccine. | human respiratory syncytial virus (rsv) is the leading cause of severe bronchiolitis and pneumonia in infants. rsv vaccine development has been stifled for the past 23 years because infants vaccinated with formalin-inactivated (fi) rsv have experienced exacerbated disease upon rsv infection. this exacerbated disease phenomenon is poorly understood, in part because of the lack of a primate model that exhibits a similar exacerbated disease state. vaccination of african green monkeys with either fi ... | 1993 | 8440926 |
| antigenic and nucleic acid analysis of nosocomial isolates of respiratory syncytial virus. | monoclonal antibodies and ribonuclease protection were used to analyze antigenic and genomic diversity among 42 isolates of group a respiratory syncytial virus (rsv) from studies of nosocomial rsv carried out at the university of rochester during the 1974-1975 and 1975-1976 rsv seasons. three distinct subgroups or lineages and a total of 12 viral variants were present. against this background of diversity, an outbreak was recognized that included 13 indistinguishable isolates occurring during a ... | 1993 | 8440927 |
| aetiology of community-acquired pneumonia in children treated in hospital. | viral and bacterial antigen and antibody assays were prospectively applied to study the microbial aetiology of community-acquired pneumonia in 195 hospitalised children during a surveillance period of 12 months. a viral infection alone was indicated in 37 (19%), a bacterial infection alone in 30 (15%) and a mixed viral-bacterial infection in 32 (16%) patients. thus, 46% of the 69 patients with viral infection and 52% of the 62 patients with bacterial infection had a mixed viral and bacterial aet ... | 1993 | 8444202 |
| risk factors for mechanical ventilation in respiratory syncytial virus infection. | in a prospective study, risk factors for mechanical ventilation were identified in 102 patients with respiratory syncytial virus (rsv) infection admitted to the sophia children's hospital, rotterdam. age, weight on admission and prematurity were associated with the need for mechanical ventilation. using multivariate analysis, only low weight was an independent risk factor for mechanical ventilation. for infants weighing less than 5 kg the relative risk for mechanical ventilation was 4.4 (95% con ... | 1993 | 8444219 |
| expression of the g glycoprotein gene of human respiratory syncytial virus in salmonella typhimurium. | the attachment protein, g, of human respiratory syncytial virus (rsv) is an m(r) 84k to 90k species which has a high content of n-linked and o-linked carbohydrates. the unglycosylated form of this protein was expressed by inserting a full-length cdna copy of the mrna from the a2 strain of rsv into a prokaryotic expression vector under the control of the lambda pl promoter. salmonella typhimurium cells transformed with the g-containing plasmid synthesized a protein of m(r) 40,000 that specificall ... | 1993 | 8445368 |
| more on rsv and ribavirin. | the description of teratogenic potential in rodents, rabbits, and hamsters from environmental exposure to ribavirin has raised concerns among caregivers. elimination of ribavirin use to avoid caregiver exposure is neither scientifically nor ethically justified because of its proven efficacy in the treatment of the potentially life-threatening disease of respiratory syncytial virus (rsv) in infants and young children. | 1993 | 8446486 |
| ribavirin effect on pulmonary function in young infants with respiratory syncytial virus bronchiolitis. | to assess the effect of ribavirin on pulmonary function in infants with respiratory syncytial virus bronchiolitis, we performed a randomized (nonmatched), double blinded, placebo-controlled study of 19 infants with rsv bronchiolitis. infants with underlying respiratory, cardiac or immunologic disease were excluded. patients were given ribavirin (10) or placebo (9) via an aerosol generator for 18 hours/day for 3 days. pulmonary function (dynamic compliance, total lung resistance) was calculated u ... | 1993 | 8451098 |
| characterization of the in vitro system for the synthesis of mrna from human respiratory syncytial virus. | an in vitro transcription system for human respiratory syncytial virus (rsv) is described. purified viral nucleocapsid (rnp) isolated from virus-infected cells was shown to support transcription of all 10 genes encoded by the virus as determined by northern blot hybridization. the mrnas synthesized were polyadenylated and comigrated with the corresponding mrnas synthesized in virus-infected cells when analyzed in agarose-urea gel electrophoresis. the in vitro-synthesized mrnas are functional as ... | 1993 | 8460489 |
| gene delivery to spinal motor neurons. | this study demonstrates the direct delivery of plasmid gene constructs into spinal motor neurons utilizing retrograde axoplasmic transport. the plasmid vectors contained the lac z gene under the control of both the rous sarcoma virus (rsv) and simian virus (sv)40 promoters. beta-galactosidase expression was observed in alpha and gamma motor neurons by histochemical staining following direct injection into the sciatic nerve or gastrocnemius muscle. the presence of lacz gene constructs was confirm ... | 1993 | 8461993 |
| evaluation of abbott testpack rsv for the diagnosis of respiratory syncytial virus infections. | abbott testpack rsv, a 20-minute enzyme immunoassay, is available for the rapid diagnosis of respiratory syncytial virus (rsv) infections. we have compared testpack with a "gold standard" method of virus isolation in traditional tube cultures and shell vials to determine the sensitivity and specificity of this rapid method. respiratory specimens were collected prospectively from 402 children and assayed by the rapid antigen detection method and isolation in culture. virus was isolated by inocula ... | 1993 | 8467621 |
| nucleotide sequence and possible ambisense coding strategy of rice stripe virus rna segment 2. | the complete nucleotide sequence (3514 nucleotides) of rna segment 2 of rice stripe virus (rsv), the prototype member of tenuivirus group, was determined. in the virus-sense rna an open reading frame (orf) is present which encodes a 199 amino acid protein of m(r) 22,762. another long orf encoding an 834 amino acid protein with m(r) 94,047 (94k) exists in the virus-complementary rna. between these two orfs, there is a long non-coding intergenic region of 299 nucleotides. the sequence suggests tha ... | 1993 | 8468559 |
| ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages. | this study was performed to evaluate the effect of ozone (o3) exposure at 1 ppm for 2 hr on the susceptibility/resistance of adult human alveolar macrophages (am) to infection with respiratory syncytial virus (rsv) in vitro and on rsv-induced cytokine production by the am. am were first exposed to o3 or to filtered air and then infected with rsv at multiplicities of infection (m.o.i.) of 0.1, 1.0, and 10. the percentage rsv-infected am and the amount of infectious virus released by the cells wer ... | 1993 | 8472647 |
| effect of respiratory syncytial virus infection on binding of neisseria meningitidis and haemophilus influenzae type b to a human epithelial cell line (hep-2). | it has been suggested that individuals might be more readily colonized with bacteria that cause meningitis through enhanced binding of the bacteria to virus-infected epithelial cells. as respiratory syncytial virus (rsv) affects infants and children in the age group also susceptible to bacterial meningitis, we tested the hypothesis that infection of hep-2 cells by rsv might enhance binding of neisseria meningitidis or haemophilus influenzae type b (hib). attachment of fluorescein-labelled bacter ... | 1993 | 8472778 |
| the rhino-probe nasal curette for detecting respiratory syncytial virus in children. | during two outbreaks of respiratory syncytial virus (rsv) infection, 68 children with acute respiratory illnesses were cultured for rsv using a rhino-probe (rp) nasal curette and either a nasopharyngeal (np) swab or a nasal wash (nw). in the first outbreak isolations of rsv by the rp nasal curette and np swab methods were compared. rsv was cultured from 25 of 42 (60%) subjects using the rp nasal curette and from 20 of 42 (48%) subjects using the np swab. in the second outbreak the rp nasal curet ... | 1993 | 8483627 |
| cytologic diagnosis of respiratory syncytial virus infection in a bronchoalveolar lavage specimen from a bone marrow transplant recipient. | cytologic examination of a bronchoalveolar lavage specimen from a 6-year-old bone marrow transplant recipient revealed pulmonary infiltrates and occasional cells containing discrete pink cytoplasmic inclusions on a may-grunwald-giemsa stain. direct immunofluorescence stains of cytospins prepared from the same specimen were positive for respiratory syncytial virus (rsv). electron microscopy revealed occasional epithelial cells with cytoplasmic inclusions composed of filamentous virions. the patie ... | 1993 | 8493953 |
| direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. | striated muscle is the only tissue found to be capable of taking up and expressing reporter genes that are transferred in the form of plasmid dna. thus, direct gene transfer is a potential method of gene therapy for the primary inherited myopathies. however, results to date have had insufficient and too variable expression to consider using direct gene transfer in human trials. we have determined that much of the variability of expression is due to nonuniform distribution of substances injected ... | 1993 | 8494924 |
| performance of the kallestad pathfinder enzyme immunoassay in the diagnosis of respiratory syncytial virus infections. | the kallestad pathfinder enzyme immunoassay (eia) for the rapid detection of respiratory syncytial virus (rsv) antigen was compared with virus culture and direct fluorescent antibody (dfa) to determine the reliability of the eia. during two consecutive winter respiratory seasons, 270 nasopharyngeal wash specimens were tested. rsv was detected in culture by the presence of cytopathic effect and/or an indirect immunofluorescence assay. the sensitivity of the pathfinder eia in comparison with isola ... | 1993 | 8495589 |
| respiratory syncytial virus-specific immunoglobulins in preterm infants. | incomplete transfer of maternal antibodies specific to respiratory syncytial virus (rsv) has been suggested as an explanation for the increased risk of rsv infections in preterm infants. antibodies directed against the two major rsv envelope glycoproteins, f and g, are protective in vitro and in vivo. our study was conducted to measure igg, igg1, igg2, and igg3 antibody titers against the rsv f and g glycoproteins in cord sera from infants born at different gestational ages. titers of neutralizi ... | 1993 | 8496762 |
| identifying bovine respiratory syncytial virus by reverse transcription-polymerase chain reaction and oligonucleotide hybridizations. | an assay to identify tissue culture cells infected with bovine respiratory syncytial virus (brsv) that utilizes reverse transcription (rt), the polymerase chain reaction (pcr), and a synthetic oligonucleotide hybridization probe has been developed. the rt-pcr assay uses a brsv-specific negative-sense oligonucleotide primer to synthesize cdna from a brsv fusion protein mrna template and another brsv-specific oligonucleotide primer (positive sense) upstream from the negative-sense primer for pcr a ... | 1993 | 8501223 |
| comparison of the vidas rsv assay and the abbott testpack rsv with direct immunofluorescence for detection of respiratory syncytial virus in nasopharyngeal aspirates. | the sensitivity and accuracy of the vidas rsv assay in testing fresh specimens were 82.7 and 87.1%, respectively, whereas specimens previously frozen at -70 degrees c gave a sensitivity of 96.2% and an accuracy of 95.4%. the sensitivity and accuracy of abbott testpack rsv were 92.6 and 91.3% for fresh specimens and 86.8 and 88.1% for frozen specimens. the advantages and drawbacks of the two assays are discussed. | 1993 | 8501239 |
| enzyme immunoassay for respiratory syncytial virus: rapid detection in nasopharyngeal secretions and evaluation of isolates representing different rsv subgroups. | the presence of respiratory syncytial virus (rsv) was investigated by immunofluorescent antibody (ifa) technique and by an enzyme immunoassay (eia) in 169 samples of nasopharyngeal secretions of infants and children with acute respiratory infections. of 31 samples positive by eia, 25 were positive by ifa. in 24 samples from a retrospective study, rsv positive by ifa and/or tissue culture isolation (tci), 22 were also positive by eia. the eia was also evaluated with 111 rsv isolates in hep2 cell ... | 1993 | 8505698 |
| mode of inhibition of respiratory syncytial virus by a plant flavonoid, sp-303. | a natural-product polyphenolic polymer of molecular weight 2,100 daltons designated sp-303, was found to have antiviral activity against respiratory syncytial virus (rsv) in the 2-10 microm range in plaque reduction assays and cytopathic-effect-inhibition assays. the material was also virucidal. the 50% effective concentration (ec50) for virucidal activity was 28 microm. experiments were done to determine the mode(s) of rsv inhibition by sp-303. interferon was not induced. sp-303 did not inhibit ... | 1993 | 8508691 |
| ambisense coding strategy of the rice stripe virus genome: in vitro translation studies. | rice stripe virus (rsv), the type species of the tenuivirus group, contains four rna segments as its genome. sequence analyses of the three smaller segments indicated that all of them have ambisense coding strategies. to examine the ambisense nature of the genomic rnas, we synthesized in vitro the rnas carrying the putative open reading frames (orfs) by transcribing cdna clones for rna segments 2, 3 and 4 in both directions using t7 rna polymerase and translated each rna in vitro using two syste ... | 1993 | 8509762 |
| viral pathogens of acute lower respiratory infections in pre-school nigerian children and clinical implications of multiple microbial identifications. | in a prospective study of acute lower respiratory infections (alri), at the university college hospital, uch, ibadan, 35 viral pathogens were identified by immunofluorescence (if) techniques from 24 (68.6%) respiratory specimens from 35 hospitalised pre-school children. the respiratory diagnoses comprised croup, bronchiolitis, pneumonia and pleural effusion. the viral identifications comprised 14 (40.0%) of parainfluenza virus type 3, 10 (28.6%) of respiratory syncytial virus (rsv), 5 (14.3%) of ... | 1993 | 8512876 |
| factors enhancing adherence of toxigenic staphylococcus aureus to epithelial cells and their possible role in sudden infant death syndrome. | toxigenic strains of staphylococcus aureus have been suggested to play a role in sudden infant death syndrome (sids). in this study we examined two factors that might enhance binding of toxigenic staphylococci to epithelial cells of infants in the age range in which cot deaths are prevalent: expression of the lewis(a) antigen and infection with respiratory syncytial virus (rsv). by flow cytometry we demonstrated that binding of three toxigenic strains of s. aureus to cells from nonsecretors was ... | 1993 | 8519316 |
| vaccination with a heterologous respiratory syncytial virus chimeric fg glycoprotein demonstrates significant subgroup cross-reactivity. | a subunit vaccine candidate, termed fg, is a chimeric glycoprotein composed of the extracellular domains of the fusion (f) glycoprotein and the attachment (g) glycoproteins of a subgroup a respiratory syncytial virus (rsv). two subgroups, a and b, of rsv differ primarily within the g glycoprotein. therefore, it has been suggested that a subunit vaccine composed of the g glycoprotein would need to contain the g glycoproteins from both rsv subgroups. we have engineered a second chimeric glycoprote ... | 1993 | 8212825 |
| controlled trial to evaluate protection of high-risk infants against respiratory syncytial virus disease by using standard intravenous immune globulin. | we performed a randomized, controlled trial of intravenous immune globulin (respiratory syncytial virus [rsv] neutralizing [nt] antibody titer of 1:950 in 5% solution) to evaluate protection against rsv-induced disease over two respiratory virus seasons. forty-nine children (mean age at enrollment, 4.5 months) with severe congenital heart disease or bronchopulmonary dysplasia were randomized as follows. twenty-four patients were followed as controls and received no immune globulin. twenty-five p ... | 1993 | 8215279 |
| modulation of pp60v-src and pp60c-src expression in rous sarcoma virus-transformed hamster fibroblasts transfected with activated n-ras. | three phenotypically different hamster cell lines transformed with rous sarcoma virus (rsv) were transfected with plasmid dna containing an activated n-ras oncogene, and nine clones expressing various levels of p21n-ras were characterized. we examined the effects of p21n-ras on expression and kinase activity of resident src proteins by using a variety of assays that allowed us to discriminate between viral and cellular src proteins. in eight clones with a 10- to 20-fold increase in p21n-ras leve ... | 1993 | 8216735 |
| [outbreak of respiratory syncytial virus at a neonatal intensive care unit in mexico city]. | rsv can be spread in hospital wards, being thus responsible for increased morbidity and mortality among infected patients. we describe an rsv outbreak in a neonatal intensive care unit (nicu). methods. as soon as the first rsv cases were detected in the nicu, every patient, as well as the personnel taking care of them, were tested; nasal washes for viral isolation, and nasopharyngeal swabs for viral antigen detection by immunofluorescence, were collected. results. twelve patients were hospitaliz ... | 1993 | 8216868 |
| presence of respiratory syncytial virus genomic sequences in middle ear fluid and its relationship to expression of cytokines and cell adhesion molecules. | the presence of respiratory syncytial virus (rsv) and several cytokines and cell adhesion molecules in middle ear effusions and mucosal tissues was evaluated using polymerase chain reaction. rsv genomic sequences were detected in 23 (52.7%) of 44 middle ear effusions tested. the sequences were detectable at an even higher rate (82.4%) in effusions of children in whom infectious virus was detected in the nasopharynx. all samples with the rsv genome contained the mrna for interleukin-1 beta and -6 ... | 1993 | 8228363 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus-infected mice with respiratory syncytial virus-specific immune serum. | the effects of passive respiratory syncytial virus (rsv) immune serum given as preinoculation prophylaxis, postinoculation prophylaxis, and as therapy on d 5 after inoculation were evaluated in an illness model of rsv infection in balb/c mice. pre- and postinoculation prophylaxis reduced rsv replication in lung after primary rsv infection and prevented illness. day 5 treatment did not affect peak titer of rsv in lung but resulted in more rapid recovery from illness than seen in untreated mice. p ... | 1993 | 8233720 |
| respiratory syncytial virus-induced cytokine production by a human bronchial epithelial cell line. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory infection in infants and young children, but the pathogenesis of rsv-induced inflammation is not well defined. we hypothesized that in vitro infection of a human bronchial epithelial cell line (beas) would induce production of proinflammatory cytokines. beas cells were infected with rsv, and cells and supernatants were assayed for cytokine mrna and protein changes at several time points after infection. cytokine mrna ... | 1993 | 8238534 |
| [experimental analysis of cytotoxicity mediated by activated macrophages against glioma cells in mice]. | macrophage (m phi) has a very important role in host natural defence and tumor cell killing. activated m phi with tumor cytotoxicity can be induced by various lymphokines, such as interferon (ifn)-gamma, tumor necrosis factor (tnf)-alpha, interleukin (il)-2, il-4, granulocyte macrophage-colony stimulating factor (gm-csf), and macrophage-colony stimulating factor (m-csf), but also by various bacterial products, such as lipopolysaccharide (lps), bacillus calmette guérin (bcg), corynebacterium parv ... | 1993 | 8239865 |
| the serum unresponsive rous sarcoma virus promoter sustains a high serum response factor-dependent transcription in vitro. | carg boxes are cis-regulatory elements which are represented both in serum responsive and unresponsive promoters. here we show that the rsv long terminal repeat contains two carg boxes, which were efficiently recognised by purified serum response factor, although they remained unresponsive to serum in transient transfection assays. however, rsv carg boxes were as efficient as c-fos serum response element in mediating a serum response factor-dependent transcription in vitro. thus, the fact that a ... | 1993 | 8240335 |
| purification and characterization of a lectin-like substance from silkworm faeces. | to obtain biologically active substances that are useful in the study of cell structures and functions, we examined several biological activities of an extract of silkworm faeces with hot buffer. we detected a lectin-like substance in the extract and purified it. when the extract of silkworm faeces was added to a culture of quail myoblasts transformed with temperature-sensitive rous sarcoma virus (qm-rsv cells), the plated cells which were cultured at 35.5 degrees c (the permissive temperature f ... | 1993 | 8242795 |
| respiratory syncytial virus lung infection in infants: immunoregulatory role of infected alveolar macrophages. | thirteen previously healthy children with acute onset of severe lower respiratory tract signs and symptoms underwent bronchoscopy and bronchoalveolar lavage (bal) for diagnostic purposes. bal samples were assessed for viral, bacterial, mycobacterial, and fungal cultures. cytospin preparations of bal cells were assessed for expression of respiratory syncytial virus (rsv), hla-dr, interleukin-1 beta, and tumor necrosis factor proteins. purified alveolar macrophages from 2 rsv-infected children wer ... | 1993 | 8245538 |
| mucosal immunization with a subunit respiratory syncytial virus vaccine in mice. | the protective efficacy of mucosal immunization with purified respiratory syncytial virus (rsv) fusion protein (f) using cholera toxin (ct) as an adjuvant was assessed in mice. intranasal vaccination with f+ct induced f-specific serum igg and nasal iga and resulted in 75% protection from nasal virus replication compared with control mice. intranasal ct+f with simultaneous parenteral f provided complete protection equivalent to intranasal live virus immunization. | 1993 | 8249433 |
| dynamics of bovine respiratory syncytial virus infections: a longitudinal epidemiological study in dairy herds. | to study the epidemiology of respiratory syncytial virus (rsv) infections during the year, the incidences of primary infections and reinfections were monitored by titrating antibodies to bovine rsv (brsv) in cattle above 2 months of age in 6 dairy herds in the netherlands. from august 1990 until september 1991, 884 cattle were sampled at one-month intervals. a total of 155 cattle, most under two years of age, had a primary antibody response. antibody rises were found in 259 cattle of all ages. t ... | 1993 | 8257292 |
| update: respiratory syncytial virus activity--united states, 1993. | respiratory syncytial virus (rsv), a common cause of community-wide outbreaks of acute respiratory disease, is associated with an estimated 90,000 hospitalizations and 4500 deaths from lower respiratory tract disease in both infants and young children in the united states. outbreaks usually occur from late fall or early winter through spring. since 1989, rsv activity in the united states has been monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, l ... | 1993 | 8259109 |
| detection of alpha-interferon in nasopharyngeal secretions and sera in children infected with respiratory syncytial virus. | we investigated the relationship between respiratory syncytial virus (rsv) antigen and interferon (ifn) in nasopharyngeal secretions (nps) and sera obtained from 252 patients infected with rsv. a total of 146 (57.9%) of 252 patients had ifn in nps with a mean titer of 28 units/ml and ifn was detected in 164 (71.6%) of 229 patients in the acute stage sera with a mean titer of 28 units/ml. ifn activities were neutralized with antiserum to ifn-alpha. rsv antigen in nps decreased on day 5 and later ... | 1993 | 8265283 |
| structural difference in the fusion protein among strains of bovine respiratory syncytial virus. | the polypeptides of different strains of bovine respiratory syncytial virus (rsv) were compared. altered electrophoretic migrations were observed in the g, f, p, m and 22 kda polypeptides. the molecular weight of the f2 fragment in human rsv (long strain) and bovine rsv (a51908 and md-x strains) was approximately 20 kda whereas it was approximately 15.5 kda in caprine rsv and bovine rsv (fs-1 and vc-464 strains). the size difference of the f2 subunit was due to difference in the extent of glycos ... | 1993 | 8273280 |
| adenoviral vector as a gene delivery system into cultured rat neuronal and glial cells. | previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (stratford-perricaudet et al., hum. gene ther., 1, 241-256, 1990; quantin et al., proc. natl. acad. sci. usa, 89, 2581-2584, 1992; jaffe et al., nature genetics, 1, 372-378, 1992). we have used a defective recombinant adenovirus, ad.rsv beta gal, containing the escherichia coli beta-ga ... | 1993 | 8275231 |
| analysis of the ovine respiratory syncytial virus (rsv) g glycoprotein gene defines a subgroup of ungulate rsv. | respiratory syncytial virus (rsv) has been isolated from sheep suffering from respiratory tract disease. since the greatest differences between bovine rsv and human rsv are found on the attachment g protein, we have determined the nucleotide and deduced amino acid sequences of the g gene of ovine rsv. the latter contained 838 nucleotides and had a major open reading frame encoding a protein of 263 residues, and shared 73% nucleotide sequence identity with that of bovine rsv. the deduced amino ac ... | 1993 | 8277288 |
| clinical severity of respiratory syncytial virus group a and b infection in sydney, australia. | respiratory syncytial virus (rsv) has two major antigenic groups, a and b. there is disagreement as to whether or not there is a difference in the clinical severity of disease caused by the two rsv groups. this 3-year prospective study of infants and children with rsv-positive bronchiolitis examines the relative virulence of rsv groups a and b and assesses the role that breast-feeding may have in modifying the clinical severity of infection. clinical severity was graded i (ventilated, severe), i ... | 1993 | 8284117 |
| production of acute bronchiolitis in guinea-pigs by human respiratory syncytial virus. | respiratory syncytial virus (rsv) is the most important cause of acute bronchiolitis in young children, and is implicated in the pathogenesis of paediatric asthma. the present studies were designed to develop a model of acute rsv bronchiolitis in young guinea-pigs, that could be used to study the mechanisms of the acute bronchiolitis and its sequelae. anaesthetized, one month old guinea-pigs received either 4 x 10(3) plaque forming units of long strain human rsv or uninfected cell culture medium ... | 1993 | 8287949 |
| enhanced c-jun activity alters responsiveness to medroxyprogesterone acetate in ishikawa human endometrial carcinoma cells. | the involvement of altered c-jun activity in medroxyprogesterone acetate (mpa)-induced growth responses in human endometrial carcinoma cells is examined in this paper. under conditions of mpa-induced growth inhibition, c-jun mrna and protein levels are decreased in ishikawa cells. this decrease is accompanied by an overall decrease in endogenous ap-1 activity in these cells. only a transient decrease in c-jun mrna level without any effect on endogenous ap-1 activity is seen in hec-50 human endom ... | 1993 | 8145769 |
| induction of interferon in human leukocyte cultures by natural pathogenic respiratory viruses. | some common viruses responsible for respiratory disease have been reported to be poor inducers of interferon (ifn). therefore, we have studied the induction of ifn in cultures of human leukocytes exposed under standardized conditions to various concentrations of adenovirus type 7a, coronavirus 229e, an influenza type a virus (h3n2), a rhinovirus, and respiratory syncytial virus (rsv). all five viruses induced substantial amounts of ifn at a multiplicity of infection of one infectious unit per ce ... | 1993 | 8151137 |
| reliable confirmation of antibodies to bovine respiratory syncytial virus (brsv) by enzyme-linked immunosorbent assay using brsv nucleocapsid protein expressed in insect cells. | the nucleocapsid (n) protein of bovine respiratory syncytial virus (brsv) in the baculovirus expression system was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (elisa) for the detection of respiratory syncytial virus (rsv) antibodies. the recombinant n protein was purified from infected-cell extracts by sucrose gradient centrifugation and used in the elisa for the detection of antibodies to various rsv strains. the elisa was compared with the virus neutralization (vn) ... | 1993 | 8308106 |
| immunoprophylaxis and immunotherapy: role in the prevention and treatment of repiratory syncytial virus. | it is now apparent that neutralizing antibody may play an important role in ameliorating rsv lower repiratory tract illness. at the present time immunoprophylaxis and immunotherapy with polyclonal antibodies show the most promise in the prevention and treatment of rsv illness. several questions remained to be answered. these include the practical application of igiv in prevention and treatment of rsv illness. intravenous lines are often difficult to place and maintain. with the further developme ... | 1993 | 18611528 |
| genereviews | glycogen storage disease type ii (gsd ii), or pompe disease, is classified by age of onset, organ involvement, severity, and rate of progression. classic infantile-onset pompe disease may be apparent in utero but more often presents in the first month of life with hypotonia, generalized muscle weakness, cardiomegaly and hypertrophic cardiomyopathy, feeding difficulties, failure to thrive, respiratory distress, and hearing loss. without treatment by enzyme replacement therapy (ert), classic infan ... | 1993 | 20301438 |
| [acute parotitis in children previously vaccinated against mumps]. | children who had previously received morbilli-(mumps) rubella (mmr) vaccine developed parotid swelling which was diagnosed as acute parotitis 7 days to 2 years following inoculation. blood samples from each of the patients were tested for the following virological parameters: mumps-virus, parainfluenza-viruses (piv) type 1., 2., 3., respiratory syncytial virus (rsv), epstein barr virus capsid antigen (ebvca) igm, iga, igg immunofluorescent test (ift) and ebvca igm, igg elisa (human); epstein bar ... | 1994 | 8309684 |
| adenovirus-mediated transfer of a human dystrophin gene to skeletal muscle of mdx mouse. | due to their quiescent nature and spatial complexity, many target tissues for gene therapy will require novel strategies. an alternative to ex vivo gene transfer, providing many technical advantages and possibly allowing sufficient transfer of the therapeutic gene, is direct in vivo delivery of the vehicle. for a favorable outcome, this procedure is dependent on a high-titer vector, fully competent before post-mitotic cells. in view of the restrictions with the use of retroviruses, we investigat ... | 1994 | 8542399 |
| respiratory syncytial virus group-specific antibody response in nasopharyngeal secretions from infants and children after primary infection. | respiratory syncytial virus (rsv) group-specific immunoglobulin a (iga) and igg enzyme-linked immunosorbent assay antibody and neutralizing antibody responses were determined for nasopharyngeal secretions (nps) from 27 infants and children (6 to 18 months of age) undergoing primary infection with rsv group a or b strain. iga and igg antibody responses against rsv envelope glycoproteins (fusion [f] and large [g] glycoprotein) in nps were also analyzed. most subjects examined developed moderate le ... | 1994 | 8556486 |
| antibody-dependent enhancement of respiratory syncytial virus infection by sera from young infants. | respiratory syncytial virus (rsv) convalescent-phase sera and control sera from both infants ( < 6 months) and older individuals (1.5 to 90 years) were assayed for rsv-specific antibodies by neutralization, in vitro enhancing activity, and immunoprecipitation. enhancement of rsv infection in u937 cells was demonstrated with convalescent-phase sera and was shown to be dependent on fc receptors by blocking with human immunoglobulin g (p < 0.01). convalescent-phase sera from infants enhanced infect ... | 1994 | 8556519 |
| isolation and characterization of a chimpanzee monoclonal antibody to the g glycoprotein of human respiratory syncytial virus. | respiratory syncytial virus (rsv) is the most common cause of serious lower respiratory tract disease in infants and young children. in this study a hybridoma line secreting a chimpanzee monoclonal antibody that neutralizes rsv was isolated. two chimpanzees were immunized with recombinant vaccinia viruses that express the rsv f or g surface glycoprotein and 1 month later were infected intranasally with the wild-type rsv strain a2. peripheral blood lymphocytes obtained from the animals were trans ... | 1994 | 8556524 |
| [thirty seven cases of respiratory syncytial virus infection hospitalised and 7 severe cases with apneic attacks]. | we studied 37 cases of respiratory syncytial virus infection hospitalized during the period january 1, 1989 and march 31, 1993. 37 patients were 0-4 years old on admission. fever, positive crp and elevation of bsg were frequent in patients aged 8 months-4 years old. patient more than 8 months of age didn't need supplementation of oxygen. of the 37 patients, we had one case who had hyponatremia and six cases who were admitted with severe apneic attacks required incubation. of the six cases, one p ... | 1994 | 8151149 |
| mutational analysis of the substrate binding pockets of the rous sarcoma virus and human immunodeficiency virus-1 proteases. | mutations, designed by analysis of the crystal structures of rous sarcoma virus (rsv) and human immunodeficiency virus type 1 (hiv-1) protease (pr), were introduced into the substrate binding pocket of rsv pr. the mutations substituted nonconserved residues of rsv pr, located within 10 a of the substrate, for those in structurally equivalent positions of hiv-1 pr. changes in the activity of purified mutants were detected in vitro by following cleavage of synthetic peptides representing wild-type ... | 1994 | 8157644 |
| [etiology of epidemic parotitis]. | blood samples of 204 acute parotitis patients in a fifteen month period (1991-1992) were systematically examined for igm, iga, igg antibodies of mumps and parainfluenza-virus 1, 2, 3 (piv) by immunofluorescent test (ift) and, in special cases several other virological examinations have been done. the etiological role of mumps-virus, parainfluenza-virus 1, 2, 3, one of the other viruses was confirmed in 76.0%, 1.5%, 6.3%, 1.5%, 4.9% of the cases, respectively. the etiology remained unknown in 9.8 ... | 1994 | 8290235 |
| cloning of rous sarcoma virus enhancer factor genes. i. evidence that rsv-ef-i is related to y-box (inverted ccaat) binding proteins and binds to multiple motifs in the rsv enhancer. | a cdna clone was isolated from a chicken embryo cdna library employing a pcr-generated radiolabeled probe specific for the u3 region of the rous sarcoma virus ltr. the cdna encodes a protein of 345 aa and is homologous to the y-box (inverted ccaat) binding proteins. the amino acid sequence of the rsv-ef-i shows 75% identity with rat ef1 but the nh2-terminal 60-aa residues share little homology. at the carboxyl terminus an additional 28-aa sequence, rich in basic residues, probably encoded by an ... | 1994 | 8291233 |
| attenuation of ribosomal protein s6 phosphatase activity in chicken embryo fibroblasts transformed by rous sarcoma virus. | in chicken embryo fibroblasts, phosphorylation of the 40s ribosomal protein s6 increases during g1 but returns to basal level by mitosis. in contrast, in rous sarcoma virus (rsv)-transformed fibroblasts, s6 remains highly phosphorylated throughout mitosis. this study investigated the mechanism by which rsv alters the pattern of s6 phosphorylation. pulse-chase experiments demonstrate that phosphate turnover in s6 is rapid in normal cells and in cells infected with an rsv transformation-defective ... | 1994 | 8264587 |
| suspension culture of hela cells in protein-free medium: sensitivity to human pathogenic viruses. | human adherent hela-pf cells grown for 5 years in a protein-free 1:1 nutrient mixture of dulbecco's modified mem and ham's f12 (dmem-f12) were established in suspension culture. the cells grew in protein-free dmem-f12 (using magnetically stirred flasks) as a monodisperse suspension with a population doubling time of 28 h. the cells were infected with poliovirus types 2 and 3, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), respiratory syncytial virus (rsv), echovirus 6 and adenoviruses 3 a ... | 1994 | 8586536 |
| gene therapy for brain tumors: regression of experimental gliomas by adenovirus-mediated gene transfer in vivo. | the therapeutic efficacy of adenovirus-mediated herpes simplex virus thymidine kinase (hsv-tk) gene transduction of rat c6 glioma cells followed by ganciclovir (gcv) administration was studied in tumors generated in the brains of nude mice. c6 glioma cells were efficiently transduced in vitro by a replicative-defective recombinant adenovirus carrying the hsv-tk gene (adv/rsv-tk) that rendered them sensitive to gcv in a dose-dependent manner. tumors were generated by stereotaxic intracerebral inj ... | 1994 | 8159705 |
| alteration of in vitro dna synthesis in the alpha globin locus of chick embryo fibroblasts due to in vivo activity of rous sarcoma virus pp60src. | globin gene transcription is activated in chick embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv). to determine whether this activation is correlated with a change in the replication of the alpha d globin locus we used a nuclear runoff replication assay. density labeling of nuclei showed that replication of the alpha d globin gene in normal and rsv-transformed cef (rsv-cef) is in the transcriptional direction. however, in rsv-cef the signal from a specific restriction fragment bet ... | 1994 | 8165153 |
| the pulmonary immune response of balb/c mice vaccinated with the fusion protein of respiratory syncytial virus. | we have investigated the efficacy of vaccination with the purified fusion (f) protein of respiratory syncytial virus (rsv) on aluminium hydroxide adjuvant in balb/c mice. the purpose of the study was to define the role of the local pulmonary mononuclear cell (pmc) infiltrate in the clearance of virus from the lower respiratory tract. balb/c mice immunized with f protein were able to inhibit the replication of virus in the lungs as early as 4 days after intranasal challenge. in contrast, unimmuni ... | 1994 | 8165859 |
| economic impact of viral respiratory disease in children. | the single most important respiratory pathogen in infancy and early childhood is respiratory syncytial virus (rsv). approximately 40% of primary rsv infections in children result in lower respiratory tract disease. approximately 1% of rsv-infected children require hospitalization. especially in high-risk children, primary rsv infection results in significant morbidity and, sometimes, death. this high-risk group includes children with bronchopulmonary dysplasia, children with congenital heart dis ... | 1994 | 8169753 |
| treatment and prevention options for respiratory syncytial virus infections. | although the therapeutic antiviral agents ribavirin and amantadine ameliorate illness caused by influenza a and respiratory syncytial virus (rsv) in children, these agents are used infrequently because they are not cost-effective. research currently is directed toward defining the high-risk groups for which these antiviral drugs should be used. treatment of severe respiratory infection with specific immune globulin, either alone or in combination with antiviral drugs, is another therapeutic appr ... | 1994 | 8169754 |
| role of antibody and use of respiratory syncytial virus (rsv) immune globulin to prevent severe rsv disease in high-risk children. | infants with cardiac disease or infants born prematurely with or without bronchopulmonary dysplasia are at increased risk of severe respiratory syncytial virus (rsv) disease. a recently developed rsv immune globulin (rsvig) was studied in a 3-year multicenter trial to determine safety and efficacy in the prevention of severe rsv disease in children who are at high risk of severe rsv illness. two hundred forty-nine children were studied; 102 had bronchopulmonary dysplasia, 87 had congenital heart ... | 1994 | 8169755 |
| salivary anti-rsv iga antibodies and respiratory infections during the first year of life in atopic and non-atopic infants. | salivary siga antibodies against rs virus were studied in 105 children during the first year of life. the infants were divided into groups according to their risk of atopy. at birth 13 neonates showed measurable amounts of siga to rs virus. in another 26 children specific antibodies were detected but in concentrations too low for quantitative analysis. during the first year of life this increased to 29 antibody-positive samples with measurable amounts of antibody and 39 with concentrations too l ... | 1994 | 8173639 |
| preparation and characterization of a monoclonal antibody that inhibits myoblast fusion of avian skeletal myoblasts. | to investigate the mechanism of myoblast fusion using quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells), we prepared monoclonal antibodies against a cell surface antigen involved in myogenic differentiation. for this, a balb/c mouse was immunized with the membrane fraction of qm-rsv cells, and hybridomas producing monoclonal antibodies were raised by fusion of spleen cells from the immunized mouse with myeloma cells. by analysis of the hybridoma ... | 1994 | 8174634 |
| synthesis and antiviral activities of 8-alkynyl-, 8-alkenyl-, and 8-alkyl-2'-deoxyadenosine analogues. | palladium-catalyzed cross-coupling of 8-bromo-2'-deoxyadenosine with terminal alkynes in the presence of copper(i) iodide in dimethylformamide resulted in a series of 8-(1-alkyn-1-yl)-2'-deoxyadenosines. hydrogenation of alkynyl derivatives over 10% pd/c under atmospheric pressure gave 8-n-alkyl analogues in nearly quantitative yields. on partial saturation of heptynyl, pentynyl, and propynyl derivatives over lindlar catalyst, the corresponding cis-olefins were obtained along with minor amounts ... | 1994 | 8176708 |
| analysis of respiratory syncytial virus f, g, and sh proteins in cell fusion. | recombinant expression of the human respiratory syncytial virus (rsv) fusion (f) glycoprotein, receptor-binding glycoprotein (g), and small hydrophobic (sh) protein was performed to determine the role(s) of these proteins in syncytia formation. these studies used a vaccinia virus expressing the bacteriophage (t7) rna polymerase gene and plasmid vectors containing the rsv genes under the control of a t7 promoter. within the context of this expression system, expression of any individual rsv gene, ... | 1994 | 8178462 |
| temporal and spatial expression of lipospermine-compacted genes transferred into chick embryos in vivo. | we have optimized a lipospermine-based transfection method for introducing genes into intact vertebrate embryos in vivo. the method employs small amounts of the cationic lipid transfectam (dogs), in a concentrated (40 mm) ethanolic solution, to compact and to transfer exogenous genes into chick embryos during the early stages of development (< 36 h of incubation). plasmid vectors containing the reporter gene luciferase were used to follow the time course of expression. luciferase activity was de ... | 1994 | 8185924 |
| evaluation of a rapid diagnostic test for respiratory syncytial virus (rsv): potential for bedside diagnosis. | rapid detection of respiratory syncytial virus (rsv) infection can assist clinicians in decisions regarding antiviral therapy with ribavirin as well as instituting infection control measures. the abbott testpack rsv is a rapid rsv detection immunoassay that can be performed on respiratory secretions in 20 to 30 minutes without special laboratory equipment. the purpose of this study was to evaluate housestaff performance of the testpack rsv at bedside as compared with laboratory testing of aliquo ... | 1994 | 8190574 |
| in vitro and in vivo inhibition of ortho- and paramyxovirus infections by a new class of sulfonic acid polymers interacting with virus-cell binding and/or fusion. | a series of sulfonic acid polymers were shown to be potent and selective inhibitors of respiratory syncytial virus (rsv) and influenza a virus. the compounds inhibit the replication of rsv and influenza a virus in hela and mdck cells, at concentrations of 0.16 and 4.0 micrograms/ml, respectively, and are nontoxic to growing cells at concentrations of > 100 micrograms/ml. the mode of antiviral action of the sulfonic acid polymers can be ascribed to inhibition of virus-cell fusion (for influenza a ... | 1994 | 8192454 |
| comparison of antibody concentrations and protective activity of respiratory syncytial virus immune globulin and conventional immune globulin. | relative to conventional immune globulins (ig, 13 lots), igs prepared from donors with high activity by microneutralization assay to respiratory syncytial virus (rsvig, 8 lots) had significantly higher neutralizing antibodies to 6 rsv strains (mean enrichment, 5.2-fold; range, 2.6- to 10.0-fold). in contrast, igg antibody concentrations to whole rsv, fusion protein, or glycoproteins of a and b strains were similar in rsvig and ig. treatment of cotton rats with rsvig at 0.5 g/kg 24 h before rsv c ... | 1994 | 8195619 |
| ribavirin administration via scavenger vacuum systems in the treatment of respiratory syncytial virus (rsv). | 1994 | 8201541 | |
| respiratory syncytial virus infection in children. | molecular epidemiologic studies have shown that during respiratory syncytial virus (rsv) epidemics several subgroups of viruses cocirculate. on the other hand, very similar strains can be found from widely separated countries at the same time. nosocomial outbreaks make no exception because multiple sources of virus have also been found within one outbreak. management of rsv infections has thus far varied largely but based on the recent reports some conclusions can be drawn. beta-sympathomimetics ... | 1994 | 8205168 |
| persistence of respiratory syncytial virus genome and protein after acute bronchiolitis in guinea pigs. | children with acute respiratory syncytial virus (rsv) bronchiolitis often develop sequelae of recurrent wheezing and asthma. to determine whether rsv persists within the lung after resolution of acute bronchiolitis, we examined the lungs of guinea pigs 60 days after intranasal inoculation with either human rsv (n = 10) or uninfected cell culture supernatant (n = 11). evidence of viral persistence within the lung was determined by viral culture to test for replicating virus, immunohistochemistry ... | 1994 | 8205887 |
| linear antigenic and immunogenic regions of the respiratory syncytial virus p protein. | three linear antigenic regions on the p protein from human respiratory syncytial virus (rsv) subgroup a (strain a2) were represented by peptides that reacted with monoclonal antibodies and with sera from humans with recent or previous rsv infection. the determinants were localized within three hydrophilic regions of the p protein: pro91 to asp110, ser161 to lys180 and glu221 to phe241. the role of individual amino acids in the epitopes defined by monoclonal antibodies was determined. two monoclo ... | 1994 | 8207401 |
| the role of rsv neutralizing antibodies in the treatment and prevention of respiratory syncytial virus infection in high-risk children. | studies assessing the use of rsv immune globulin in the treatment of rsv illness are not yet completed. however, a large multicenter trial demonstrated that prophylaxis with rsv immune globulin was safe and efficacious in prevention of serious rsv disease in high-risk infants. refinements in the practical application of rsvig are needed, as intravenous lines are difficult to place and maintain in these fragile infants. with the development of concentrated polyclonal and/or effective monoclonal a ... | 1994 | 8141588 |
| two family outbreaks of chlamydia pneumoniae infection. | during autumn 1992, we observed two unrelated family outbreaks of chlamydia pneumoniae infection. family a consisted of grandmother (aged 77 yrs), father (aged 41 yrs), mother (aged 38 yrs), daughter (aged 10 yrs), and two sons (aged 6 yrs and 3 months, respectively). the grandmother and daughter suffered from pneumonia, father from pharyngitis and bronchitis and the older son from mild bronchitis. no symptoms were recorded in the mother and younger son. symptomatic subjects showed a fourfold in ... | 1994 | 8143807 |
| [virus infections--disease manifestations and laboratory diagnosis. part 6: mumps virus--respiratory syncytial virus (rsv)]. | 1994 | 8144123 | |
| [epidemiological study on respiratory syncytial virus lower respiratory tract infections in northern hokkaido, japan]. | we carried out an epidemiological study on lower respiratory tract infections, concerning the respiratory syncytial virus (rsv) infection, in northern hokkaido, japan, during the 2-year-study period from april 1991 to march 1993. there were 467 hospitalized patients with lower respiratory tract infections. we examined the presence of rsv antigen in their nasopharyngeal specimens of 456 out of 467 patients (97.6%) by enzyme immunoassay. the diagnosis of mycoplasma pneumoniae infection was done by ... | 1994 | 8138666 |
| rous sarcoma virus integrase protein: mapping functions for catalysis and substrate binding. | rous sarcoma virus (rsv), like all retroviruses, encodes an integrase protein that is responsible for covalently joining the reverse-transcribed viral dna to host dna. we have probed the organization of functions within rsv integrase by constructing mutant derivatives and assaying their activities in vitro. we find that deletion derivatives lacking the amino-terminal 53 amino acids, which contain the conserved h-x(3-7)-h-x(23-32)-c-x(2)-c (hhcc) zn(2+)-binding motif, are greatly impaired in thei ... | 1994 | 8139006 |
| interactions between marek's disease virus encoded or induced factors and the rous sarcoma virus long terminal repeat promoter. | marek's disease virus transactivates promoters of avian leukemia and sarcoma viruses. in this study, a series of rsv-ltr promoter deletion mutants were used to map sites within the ltr important for mdv-mediated transactivation. mdv-responsive elements within rsv-ltr promoters were localized to a 28-bp segment (nucleotides -109 to -137) which contains a ggtgg pentanucleotide repeat element (pre). nuclear extract proteins from uninfected cells bound to the rsv pre in a sequence-specific manner. e ... | 1994 | 8116231 |
| lower respiratory viral infections in immunocompetent children. | viral lower respiratory disease causes a heavy burden on our society. better understanding of the epidemiology of these viruses combined with new rapid diagnostic techniques will provide more rapid and more reliable diagnosis of these agents in the future. two agents not commonly thought of as causes of lri in children (rhinoviruses, coronaviruses) should now be added to an already long list. effective drugs exist for prophylaxis against influenza virus type a and therapy for influenza virus typ ... | 1994 | 8123226 |
| respiratory syncytial virus subgroup b dominance during one winter season between 1987 and 1992 in vancouver, canada. | a subgroup analysis of 613 specimens submitted to the british columbia's children's hospital from 1987 to 1992 revealed that subgroups a and b of respiratory syncytial virus (rsv) were both circulating in our community, with some predominance for subgroup a during the period from october 1987 to september 1988 (the 1987-88 season) (64%), 1990-91 (60%), and 1991-92 (62%). during 1989-90 subgroup a represented the majority of isolates (80%). subgroup b predominated during only one season, 1988-89 ... | 1994 | 8126189 |
| alteration of in vivo dna synthesis in the alpha globin locus of chick embryo fibroblasts due to in vivo activity of rous sarcoma virus pp60src. | globin gene transcription is activated in chick embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv). to determine whether this activation is correlated with a change in the replication of the alpha d globin locus we used a nuclear runoff replication assay. density labeling of nuclei showed that replication of the alpha d globin gene in normal and rsv-transformed cef (rsv-cef) is in the transcriptional direction. however, in rsv-cef the signal from a specific restriction fragment bet ... | 1994 | 8127691 |
| recombinant human respiratory syncytial virus (rsv) monoclonal antibody fab is effective therapeutically when introduced directly into the lungs of rsv-infected mice. | previously, recombinant human respiratory syncytial virus (rsv) monoclonal antibody fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. two such fabs, which exhibited high binding affinity for rsv f glycoprotein (a major protective antigen), were evaluated for therapeutic efficacy in infected mice just before or at the time of peak virus replication in the lungs. fab 19, which neutralized rsv infectivity with high efficiency in ... | 1994 | 8108420 |
| nucleotide and predicted amino acid sequence analysis of the ovine respiratory syncytial virus non-structural 1c and 1b genes and the small hydrophobic protein gene. | respiratory syncytial virus (rsv) infects humans and cattle causing serious respiratory tract disease in both. the genome of the human and bovine rsv (hrsv and brsv) codes for two non-structural and eight structural proteins. rsv has also been isolated from naturally infected sheep, but the genome of the ovine rsv (orsv) has not been characterized and nor has the virus host range been identified. here we report on the cloning and sequencing of the two non-structural 1c and 1b genes and of the sm ... | 1994 | 8113762 |
| effect of maternal antibody on iga antibody response in nasopharyngeal secretion in infants and children during primary respiratory syncytial virus infection. | the iga antibody response to respiratory syncytial virus (rsv) was determined in nasopharyngeal secretions (nps) of 22 infants and children infected with rsv group a strains, employing an elisa. the antibody activity observed during the convalescent phase against whole virus, fusion glycoprotein (f) and large glycoprotein (g) was examined in young infants (under 6 months) and compared with that of older individuals (6 to 16 months). both groups showed similar degrees of iga antibody activity to ... | 1994 | 8046418 |
| antigenic diversity of respiratory syncytial virus subgroup b strains circulating during a community outbreak of infection. | the epidemiological characteristics and relationship between respiratory syncytial virus (rsv) subgroup and virulence during an outbreak of rsv infection occurring in southeast texas in the winter season 1991/92 are described. fifty-two infants and children were diagnosed with rsv infection by rapid viral antigen detection and/or viral isolation. subgrouping of the isolates was carried out using 11-monoclonal anti-bodies. ten isolates were found to be subgroup b, and 8 isolates were subgroup a. ... | 1994 | 8046428 |
| effects of the gag region on genome stability: avian retroviral vectors that contain sequences from the bryan strain of rous sarcoma virus. | we have previously described replication-competent schmidt ruppin-a rous sarcoma virus (rsv)-based retroviral vectors that can be used to deliver and express genes in avian cells. we have continued to modify the prototype vectors to develop a more versatile and efficient system. substitution of the polymerase (pol) region from the bryan high-titer rsv (bh-rsv) for the sr-a rsv pol region of these retroviral vectors causes these viruses to replicate more efficiently. we cloned the gag regions fro ... | 1994 | 8053145 |
| epithelial interleukin-11. regulation by cytokines, respiratory syncytial virus, and retinoic acid. | interleukin-11 (il-11) is a pleiotropic cytokine with effects that overlap with il-6. to determine if il-11 is produced by epithelial cells, we determined whether human alveolar a549 cells and airway 9hte cells produce il-11. we also determined whether retinoic acid (ra) altered this il-11 production. unstimulated cells produced low levels of il-11, while il-1, transforming growth factor (tgf-beta 1), and respiratory syncytial virus (rsv) stimulated il-11 protein production and mrna accumulation ... | 1994 | 8071352 |
| synthesis and antiviral activity evaluation of some aminoadamantane derivatives. | the synthesis of some spiro[cyclopropane-1,2'-adamantan]-2-amines and methanamines and some spiro[pyrrolidine-2,2'-adamantanes] is described. the title compounds were evaluated against a wide range of viruses (influenza a, influenza b, parainfluenza 3, rsv, hsv-1, tk- hsv-1, hsv-2, vaccinia, vesicular stomatitis, polio 1, coxsackie b4, sindbis, semliki forest, reo 1, hiv-1, and hiv-2), and some of them (compounds 6b, 6c, 9a, 16a, 16b, and 17) inhibited the cytopathicity of influenza a virus at a ... | 1994 | 8071937 |
| passive protection against respiratory syncytial virus disease in infants: the role of maternal antibody. | respiratory syncytial virus (rsv) is responsible for serious respiratory disease in young infants. more than 75% of the 678 children hospitalized for rsv at baylor-affiliated hospitals in houston, tx, between october, 1992, and march, 1993, were 5 months of age or younger. the importance of maternal antibody in the immunity against rsv disease has been debated. more recent epidemiologic studies have demonstrated protection against rsv in babies born to mothers with high levels of neutralizing rs ... | 1994 | 8072835 |
| role of antibody and the use of respiratory syncytial virus immunoglobulin in the prevention of respiratory syncytial virus disease in preterm infants with and without bronchopulmonary dysplasia. | respiratory syncytial virus (rsv) is the only viral respiratory pathogen that produces an annual epidemic of respiratory illness. infants with cardiac disease or infants born prematurely with or without bronchopulmonary dysplasia are at increased risk of severe rsv disease. a recently developed rsv immunoglobulin (rsvig) was studied to determine safety and efficacy in prevention of severe rsv disease in such children who are high risk for severe rsv illness. results from this prospective, blinde ... | 1994 | 8072836 |
| response of six major histocompatibility (b) complex recombinant haplotypes to rous sarcomas. | six b complex recombinants, br1 (f24-g23), br2 (f2-g23), br3 (f2-g23), br4 (f2-g23), br5 (f21-g19), and br6 (f21-g23), from the fourth backcross generation to highly inbred line ucd 003 (b17b17) were studied for their response to rous sarcomas. eight hatches were produced from heterozygous (brnb17) parents. chicks were wingweb inoculated with 50 pock-forming units of rous sarcoma virus (rsv) at 6 wk of age. a tumor profile index (tpi), based on degree of tumor regression, was evaluated by analys ... | 1994 | 8072926 |