Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cellular and humoral immune responses in rats experimentally infected with plasmodium berghei. | 1981 | 7040220 | |
| host defenses in murine malaria: analysis of plasmodial infection-caused defects in macrophage microbicidal capacities. | macrophage-dependent killing of facultative intracellular bacteria was markedly impaired by overt erythrocytic plasmodium yoelii or plasmodium berghei infection of mice. p. yoelii infection was capable of ablating not only the macrophage microbicidal capacity of "normal" animals but also the bactericidal capacities of "activated" macrophages. the uptake by spleen and liver of an intravenous challenge of listeria monocytogenes was not altered by plasmodial infection, but within hours of injection ... | 1981 | 7012000 |
| effect of route of mycobacterium bovis bcg administration on induction of suppression of sporozoite immunity in rodent malaria. | intravenous immunization of mice with 16,000, 60co--gamma-irradiated, attenuated sporozoites produced solid immunity to sporozoite-induced malaria when the mice were challenged 21 days after immunization. in contrast, mice injected by various routes with 10(7) viable units of mycobacterium bovis (bcg) before immunization with irradiated sporozoites were not completely immune to challenge. the extent of reduced protection against viable sporozoites demonstrated with these animals was dependent up ... | 1981 | 7012001 |
| complement does not facilitate plasmodial infections. | the influence of the complement (c) system on plasmodial infections in vivo (plasmodium berghei in rats) and in vitro (plasmodium falciparum) has been determined. in rats c3 depletion by treatment of animals with the c3 inactivator isolated from cobra venom factor results in infection that develops more rapidly, reaches a higher peak of parasitemia and is associated with an increased mortality rate (60%), in contrast to a lower degree of parasitemia and lack of mortality in c3-intact rats. the i ... | 1981 | 7012239 |
| an ultrastructural study of developing stages of exo-erythrocytic plasmodium berghei in rat hepatocytes. | the ultrastructure of immature exo-erythrocytic forms of plasmodium berghei in rat hepatocytes was studied at stages between 25 and 51 h of development. a new method was successfully applied to localize the parasites in a small portion of the liver by temporary ligature of blood vessels to the majority of the liver, and from the spleen and the pancreas. nuclear profiles appeared to be part of a highly lobed nuclear reticulum. peripheral vesiculation and vacuolization of the cytoplasm was increas ... | 1981 | 7012764 |
| plasmodium berghei: glycolytic enzymes of the infected mouse erythrocyte. | 1981 | 7014240 | |
| [some patterns of the in vivo plasmodium berghei entry into erythrocytes as revealed by scanning electron microscopy (author's transl)]. | 1981 | 7011156 | |
| quantification of hyperbaric oxygen-induced toxicity utilizing a malarial system. | this study was undertaken in recognition of the need to develop quantitative systems to evaluate the toxicity associated with hyperbaric oxygen (hbo) exposure. malaria-infected (p. berghei berghei) mice were briefly exposed to 100% oxygen at 3 ata on day 10 of infection. at 25, 48, and 72 h thereafter, the levels of circulating erythrcytes and percent parasitized rbc were monitored and compared to those of infected non-exposed controls. the total erythrocyte counts of the infected hbo-exposed an ... | 1981 | 7011300 |
| permethyl analogue of the pyrrolic antibiotic distamycin a. | the synthesis of an analogue of distamycin a, a pyrrolic oligopeptide possessing antiviral and antibiotic activity, is described in which each of the three pyrrole rings is fully methylated. this structural modification results in pyrrole rings which are extraordinarily electron rich and required the development of a new synthetic approach to these polypyrrolic amides. the key reactions involved development of a general method for the synthesis of 3-aminopyrroles and for formation of an amide bo ... | 1981 | 7009865 |
| synthesis and antimalarial effects of n2-aryl-n4-[(dialkylamino)alkyl]- and n4-aryl-n2-[(dialkylamino)alkyl]-2,4-quinazolinediamines. | a series of n2(and n4)-aryl-n4(and n2)-[(dialkylamino)alkyl]-2,4-quinazolinediamines has been synthesized for antimalarial evaluation. condensation of the appropriate 2,4-dichloroquinazoline (iv) with the requisite n,n-dialkylalkylenediamine afforded a series of 2-chloro-n-[(dialkylamino)alkyl]-4-quinazolinamines (v) which were condensed with the appropriate arylamine to provide the corresponding n2-aryl-n4-[(dialkylamino)alkyl]-2,4-quinazolinediamines (vi). hydrolysis of 2,4-dichloroquinazoline ... | 1981 | 7009867 |
| folate antagonists. 18. synthesis and antimalarial effects of n6-(arylmethyl)-n6-methyl-2,4,6-pteridinetriamines and related n6,n6-disubstituted 2,4,6-pteridinetriamines. | n6-(arylmethyl)-n6-methyl-2,4,6-pteridinetriamines (1-15) and related n6-substituted 2,4,6-pteridinetriamines (16-20) were obtained by the condensation of 6-chloro-2,4-pteridinediamine with n-methylarylmethanamine and other selected secondary amines. the requisite n-methylarylmethanamines (21-32) were prepared by the hydrogenation over pt/c of the corresponding arylcarboxaldehyde in the presence of methanamine. several of the n6-(arylmethyl)-n6-methyl-2,4,6-pteridinetriamines exhibited exception ... | 1981 | 7009868 |
| effect of plasmodium berghei on membranes of murine erythrocytes. | membranes from normal and plasmodium berghei-infected mice were analyzed to determine: 1) if any antigenic changes were present; and 2) the nature of these changes. polyacrylamide gel electrophoresis (page), immunological and biochemical analyses were performed on whole ghosts and glycoprotein fractions extracted from whole ghosts by chloroform-methanol. page profiles and biochemical analyses revealed quantitative, but not qualitative, differences between membrane proteins and glycoproteins of n ... | 1981 | 7020448 |
| host defenses in murine malaria: nonspecific resistance to plasmodium berghei generated in response to mycobacterium bovis infection or corynebacterium parvum stimulation. | infection with mycobacterium bovis (bcg) or injection of killed corynebacterium parvum protected some strain b6d2 f1 (c57bl/6xdba/2) mice but did not protect strain icr or a mice from lethal challenge with plasmodium berghei strain nyu-2. b6d2 mice were not protected against challenges delivered immediately after intravenous injection of these materials, but rather protection developed by day 7 and persisted through at least day 84. infections in protected mice progressed to about 10% parasitemi ... | 1981 | 7021424 |
| endotoxin-induced serum factor kills malarial parasites in vitro. | we investigated the possibility that malarial parasites may be killed by nonspecific soluble mediators, such as those in tumor necrosis serum, that are obtained from mice given macrophage-activating agents like corynebacterium parvum or mycobacterium bovis bcg, followed by endotoxin. such sera killed parasites in vitro after overnight incubation; killing was measured directly by using an in vivo infectivity assay. parasite infectivity was not decreased by incubation in sera from mice given c. pa ... | 1981 | 7021427 |
| autoantibodies to red blood cells in rats infected with plasmodium berghei. | a radio-iodinated protein a (125spa) binding assay was used to identify autoantibodies to red blood cells (rbc's) in sera of rats infected with plasmodium berghei. sera taken from rats at various times after infection were reacted with washed, normal rbc's, then the rbc's were washed and treated with 125spa. the bound radioactivity was taken as a measure of the amount of ig attached to the rbc's membranes. using this test, anti-rbc autoantibodies were detected in rat sera as early as 5 days afte ... | 1981 | 7021787 |
| in vitro cultivation of the exoerythrocytic stage of plasmodium berghei from sporozoites. | when inoculated with sporozoites of plasmodium berghei, the human embryonic lung cell line wi38 supports the complete asexual developmental cycle of the exoerythrocytic stage. cultured parasites were sensitive to primaquine, were shown to resemble parasites in living hosts by immunofluorescent antibody tests, and on subinoculation into mice induced a red blood cell infection, the gametocytes of which produced sporozoites in anopheline mosquitoes. | 1981 | 7022652 |
| babesia rodhaini and plasmodium berghei. a highly active series of chlorophenoxyalkoxy-substituted diamino-dihydrotriazines against experimental infections in mice. | 1981 | 7023398 | |
| chloroquine resistant malaria: association with enhanced plasmodial protease activity. | 1981 | 7023481 | |
| plasmodium berghei: effect of carrageenan on the course of infection of the a/j mouse. | 1981 | 7024158 | |
| [general characteristics of the chemotherapeutic properties of a new experimental antimalarial preparation dabequin]. | 1981 | 7024771 | |
| [hemostatic system disorders in malaria (a review of the literature)]. | 1981 | 7024773 | |
| [search for new antimalarial compounds (a review of patents)]. | 1981 | 7024774 | |
| adoptive transfer of immunity to plasmodium berghei after busulfan and cyclophosphamide treatment of recipient mice. | balb/c mice injected with p. berghei die about 21 days after infection. successful cell transfer in mice was made possible by the pretreatment of the recipient with a combination of busulfan and cyclophosphamide. cell counts showed that drug-treated mice contain 20 times less bone marrow cells than normal mice, and when injected with p. berghei die significantly later than normal controls. the animals were injected with normal (nbm) and immune bone marrow and normal (nsp) and immune spleen (isp) ... | 1981 | 7025493 |
| [complex morphologic modifications observed on the surface of red blood cells parasitized by plasmodium berghei (1)]. | 1981 | 7025979 | |
| blood schizontocidal activity of antibiotics against plasmodium berghei sensitive as well as resistant to chloroquine, pyrimethamine and primaquine. | 1981 | 7026431 | |
| biosynthesis of pb44, the protective antigen of sporozoites of plasmodium berghei. | in a previous paper (2) we identified a protective antigen (pb44) of the surface membrane of sporozoites of plasmodium berghei by means of a monoclonal antibody. immunoprecipitation of extracts of mature salivary gland sporozoites, metabolically labeled with l[35s]methionine using the same monoclonal antibody, revealed three specific polypeptides: *pb44, *pb52, and *pb54. metabolically labeled *pb44 is probably identical to the protective antigen previously identified by surface labeling. both p ... | 1981 | 7026723 |
| chloroquine resistant p. berghei: association with variation in plasmodial protease activity. | 1981 | 7027269 | |
| hemin lyses malaria parasites. | malaria parasites isolated from mouse erythrocytes are lysed by ferriprotoporphyrin ix chloride (hemin) or by a chloroquine-hemin complex in amounts that could be produced by release of less than 0.1 percent of the heme in erythrocytic hemoglobin. this effect of hemin may explain the protection against malaria provided by thalassemia and other conditions causing intracellular denaturation of hemoglobin. the toxicity of the chloroquine-hemin complex may explain the selective antimalarial action o ... | 1981 | 7027441 |
| [oxidative and antioxidative system of the blood in mechanisms of erythrocyte protection and damage upon invasion by plasmodium berghei]. | in the course of experimental infection with pl. berghei, the mice demonstrated an abrupt lowering in the activity of blood enzymes (superoxide dismutase and glutathione peroxidase) and a rise in the concentration of the lipid peroxidation product (malonic dialdehyde) with the growth of parasitemia. the evidence obtained are discussed in the light of the defence and injury blood mechanisms of red cell membranes. | 1981 | 7028174 |
| immunosuppression in murine malaria: a soluble immunosuppressive factor derived from plasmodium berghei-infected blood. | mice infected with plasmodium berghei have a depressed immune response to a variety of antigens. we report the extraction, purification, and characterization of a soluble immunosuppressive substance derived from p. berghei-infected mouse blood. a crude extract, prepared by solubilization of infected erythrocytes in a parr cell disruption bomb, reduced the anti-dnp pfc response of mice injected with the extract 1 day before immunization. purification of the immunosuppressant was accomplished by p ... | 1981 | 7028864 |
| treatment of rodent malaria with levamisole. | 1981 | 7028880 | |
| malarial antibodies and parasitaemias in immunosuppressed mice. | 1981 | 7028884 | |
| atypical reticulocytes in rats with malaria as a possible consequence of the pitting function of the spleen. | 1981 | 7030237 | |
| synthesis of some novel amodiaquine analogues as potential antimalarial and antifilarial compounds. | ten amodiaquine analogues, which are hybridized molecules of amodiaquine and diethylcarbamazine, were designed and synthesized. six analogues, all bearing a basic tertiary amino function at their side chain, were active against plasmodium berghei in mice and inhibited the mobility of adult worms and microfilariae of breinlia booliati in vitro. they were inactive against litomosoides carinii in mastomys natalensis. the most active antimalarial compound, 7-chloro-4-[alpha-[[n-(4-methyl-1-piperazin ... | 1981 | 7031248 |
| studies on the infectivity of plasmodium berghei sporozoites in experimental hosts. | 1981 | 7032427 | |
| sustained-release implants in the chemotherapy of experimental rodent malaria i. a comparison of the effects of some antimalarials in polydimethylsiloxane matrices. | 1981 | 7032430 | |
| sustained-release implants in the chemotherapy of experimental rodent malaria ii. the effects of sulphadiazine, pyrimethamine and cycloguanil in biodegradable polymer matrices. | 1981 | 7032431 | |
| leishmania donovani, plasmodium berghei, trypanosoma rhodesiense: antiprotozoal effects of some amidine types. | 1981 | 7032963 | |
| [synthesis of benzo(g)quinoline derivatives. xv. di[benzo(g)-quinolylpiperazinyl]alkanes possessing antimalarial activity]. | 1981 | 7033752 | |
| [erythrocyte enzymes at different stages of plasmodium berghei schizogony]. | 1981 | 7033753 | |
| dietary suppression of rodent malaria. | 1981 | 7034320 | |
| [morphological incidences of plasmodium berghei preference for reticulocytes (author's transl)]. | 1981 | 7034621 | |
| role of a serum factor in enhancement of in vitro interactions between plasmodium berghei sporozoites and hamster peritoneal macrophages. | interactions between plasmodium berghei sporozoites and hamster peritoneal macrophages were studied. hamster serum was shown to enhance the percentage of sporozoites tha attached to macrophages, thus confirming previous studies by other workers using mouse macrophages and mouse serum. the enhancement factor within hamster serum was concentrated by a fractionation procedure consisting of ammonium sulfate precipitation followed by concanavalin a-sepharose affinity chromatography. this serum fracti ... | 1981 | 7035641 |
| syntheses of 9-acridine- and 2-phenanthridinemethanols as potential antimalarials. | alpha-(1-piperidinylmethyl)-9-acridinemethanol (3), alpha-[(dibutylamino)ethyl]-9-acridanmethanol (4a), and alpha-[(dibutylamino)methyl]-2-phenanthridinemethanol (5) have been made and all are ineffective as antimalarials against plasmodium berghei in mice. 9-acridinyloxirane showed no significant mutagenicity for strains ta 98 or ta 100 of salmonella typhimurium. | 1981 | 7035667 |
| plasmodium berghei: the in vitro immune response. | 1981 | 7007068 | |
| immunity to plasmodium chabaudi adami in the b-cell-deficient mouse. | immunity to malaria has a multicomponent basis which requires the participation of both t- and b-lymphocyte systems. previous studies have suggested that the t-lymphocyte system has an essential role in 're-infection immunity' to malaria, but that b cells and/or their products are necessary for the host to survive acute infection and to clear the blood of parasites during chronic malaria. thus, b-cell-deficient mice and chickens died of fulminant malaria when infected with plasmodium yoelii and ... | 1981 | 6970898 |
| development of new derivatives of primaquine by association with lysosomotropic carriers. | on the basis of the drug-carrier concept of chemotherapy, we entrapped primaquine in liposomes, and linked it to an amino acid (leucine), and to peptides (alanyl-leucine and alanyl-leucyl-alanyl-leucyl) as intermediate steps in the synthesis of covalent primaquine-glycoprotein conjugates that would be selectively recognized by hepatocytes.the therapeutic activity of these compounds was tested in mice infected with sporozoites of plasmodium berghei. causal prophylatic cures were obtained after a ... | 1981 | 6976852 |
| new tissue schizontocidal antimalarial drugs. | over 700 causal prophylactic and radical curative antimalarial drugs have been discovered during the screening of approximately 4000 chemical compounds in rodent and simian malaria models. causal prophylactic activity in the plasmodium berghei-rodent model was demonstrated by 10 distinct groups of chemicals: 1) tetrahydrofolate dehydrogenase inhibitors, 2) naphthoquinones, 3) dihydroacridinediones, 4) tetrahydrofurans, 5) guanylhydrazones, 6) analogues of clopidol, 7) quinoline esters, 8) dibenz ... | 1981 | 6976854 |
| the protective antigen of malarial sporozoites (plasmodium berghei) is a differentiation antigen. | pb44, the protective antigen of rodent malaria sporozoite (plasmodium berghei) covers the entire surface of mature, salivary gland sporozoites. this antigen is undetectable in approximately 50% of the immature, i.e., oocyst sporozoites. on the surface of the remaining oocyst sporozoites, pb44 is found in patches. pb44 is present in early exoerythrocytic liver stages of p. berghei but is present in early exoerythrocytic liver stages of p. berghei but becomes undetectable after 30 hr of intrahepat ... | 1981 | 6785357 |
| glycolytic metabolism in malaria infected red cells. | 1981 | 6457304 | |
| differential involvement of non-specific suppressor t cells in two lethal murine malaria infections. | the suppression of the contact sensitivity of oxazolone in murine malaria is shown to be mediated by non-specific t suppressor cells, but to a different extent in infection caused by two different species of parasite. depletion of t suppressor cells in vivo and/or anti-thy 1.2 treatment in vitro indicated that in mice infected with p. berghei the suppressor effect was largely mediated by t cells. by contrast, in mice infected with a lethal strain of p. yoelii it was only partly due to t cells; b ... | 1981 | 6459199 |
| complement-mediated defect in clearance and sequestration of sensitized, autologous erythrocytes in rodent malaria. | we investigated the ability of malaria-infected and normal mice to clear particulate immune complexes consisting of autologous erythrocytes sensitized with either igg or complement. normal mice rapidly clear autologous erythrocytes optimally sensitized with igg and the liver plays a major role in their sequestration. clearance of optimally sensitized erythrocytes is complement-dependent because cobra venom factor-treated, normal mice failed to clear these cells rapidly, unless they had been pre- ... | 1981 | 7005263 |
| plasmodium berghei: modification of sialic acid on red cells from infected mouse blood. | 1981 | 6257538 | |
| pyrimidine biosynthesis in plasmodium berghei. | 1981 | 6260538 | |
| a simple method for separation of uninfected erythrocytes from those infected with plasmodium berghei and for isolation of artificially released parasites. | rat erythrocytes infected with plasmodium berghei were disrupted by gentle passage of concanavalin a (con a) agglutinated cells through a 100 mesh stainless steel grid. the free parasites were separated from cell debris, unbroken infected cells, and from uninfected rat erythrocytes on a percoll gradient. the parasites remained morphologically intact, metabolically active, and infective to mice. the parasites were observed by light and electron microscopy. the incorporation of 3h-isoleucine and 3 ... | 1981 | 6261470 |
| cyclic amp metabolism in p. berghei infected murine red cells. | 1981 | 6270696 | |
| comparative studies on dihydroorotate dehydrogenase from p. berghei and the mouse reticulocyte. | kinetic parameters on dihydroorotate dehydrogenase (dho-dhase) from the rodent malarial parasite, plasmodium berghei, have been determined. this enzyme, the fourth in de novo pyrimidine biosynthesis, is particulate and is absent in the mature mammalian red cell. the km of the substrate, dihydroorotate, was determined to be 23 microm and the ki values for a number of substrate analogues have been determined. the most potent inhibitor was dihydroazaorotate (ki, 3 microm), 5-azaorotate (ki, 20 micr ... | 1981 | 6271112 |
| inhibition of p. falciparum growth in human erythrocytes by monoclonal antibodies. | malaria is increasing in incidence and prevalence in most tropical areas and is a major problem for both individuals and communities. current malaria research is aimed at developing vaccines and, for this, it may be useful to define plasmodium antigen(s) related to the development of a protective immune response in the host. monoclonal antibodies have recently been shown to interfere with rodent malaria infection (plasmodium berghei) at the sporozoite or merozoite stage. we have now raised monoc ... | 1981 | 6161311 |
| monoclonal antibodies to stage-specific, species-specific, and cross-reactive antigens of the rodent malarial parasite, plasmodium yoelii. | eighteen hybridoma cell lines were used to study species-specific, stage-specific, and serological cross-reactive antigens of the rodent malarial parasite, plasmodium yoelii. specificity and location of plasmodial antigens were determined by indirect fluorescent-antibody analysis. results showed that a minimum of 12 distinct plasmodial antigens could be distinguished by the 18 hybridomas. antigens were found on the surface or within the cytoplasm of the parasite, but not on the surface of erythr ... | 1981 | 6166558 |
| immunodepression of thymus-independent response to dextran in mouse malaria. | the thymus-independent antibody response to the alpha 1-6 epitope of dextran b512 was depressed strongly during acute non-lethal plasmodium yoelii yoelii malaria, but not during low-grade chronic plasmodium berghei infection. in the acute infection, which is self-limiting, the duration of severe immunodepression was short and was seen only in mice immunized at or around the time of peak parasitaemia. mice primed at this time responded normally to challenge 20 days later: thus the primary exposur ... | 1981 | 6167386 |
| protective reaction against malaria infection in mice sensitized with frozen-thawed toxoplasma tachyzoites. | mice sensitized with frozen-thawed toxoplasma antigen emulsified in freund's incomplete adjuvant (fia) showed high resistance against plasmodium berghei infection, but not in mice sensitized with paraformaldehyde-fixed. toxoplasma or saline plus fia. however, mice which survived from malaria infection did not show any protective reaction against the rh strain inoculation. furthermore, an immune interferon activity was detected in the supernatant of the spleen cells collected from the mice sensit ... | 1981 | 6171952 |
| [study of the glycolysis of erythrocytes by the method of automatic titration of lactic acid]. | 1981 | 6172639 | |
| the influence of dietary protein on the development of malaria. | in the course of malarial infection in an animal model, the dietary level of protein proved to be important. synthetic diets were used, identical in every respect other than the type and amount of protein. reducing the protein content of the diet led to a decrease in the level of infection and a protein-free diet almost totally suppressed the disease. these findings were obtained in rats infected with either plasmodium berghei or plasmodium vinckei; when the dietary protein was in the form of ca ... | 1981 | 6185057 |
| the enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths. | the activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in crithidia fasciculata, trypanosoma cruzi, leishmania major, trichomonas vaginalis, eimeria tenella, toxoplasma gondii, plasmodium berghei, fasciola gigantica, schistosoma mansoni, hymenolepis diminuta, nippostrongylus brasiliensis and trichuris muris. the m ... | 1981 | 6111750 |
| rat malarial glomerulonephritis. an experimental model of post-infectious glomerular injury. | this paper describes the immunopathologic findings in acute malaria-associated glomerulonephritis in the rat. young sprague-dawley rats were infected with plasmodium berghei berghei. the subsequent parasitemia and elevation of circulating clq-reactive immune complexes were transient while the appearance of anti-plasmodial antibody in the serum was persistent. sequential examination of renal tissue and urine revealed the following glomerular alterations: (a) granular, predominantly mesangial depo ... | 1981 | 6117979 |
| inhibition of idiotype--anti-idiotype interaction for detection of a parasite antigen: a new immunoassay. | described in this report is an immunoradiometric assay of general applicability that is based on a new principle: the inhibition of the interaction between monoclonal antibodies by an antigen. the advantages of this assay are that it measures concentrations of single epitopes, purified antigen is not required, and the reagents can be obtained in unlimited amounts and are homogeneous. its features are particularly attractive when the antigen has not been purified and is a minor component of a com ... | 1982 | 6122269 |
| retention of plasmodium berghei sporozoites within perfused mouse livers. | a mouse liver perfusion model was adapted to evaluate the efficiency of the liver in retaining plasmodium berghei sporozoites. specific numbers of sporozoites were perfused into each liver via a portal vein cannula. the numbers of sporozoites in the perfusate effluent were counted and the percent sporozoite retention calculated. over 95% of sporozoites suspended in medium with plasma were retained in a normal liver following a single passage. sporozoites were seen in sinusoids of perfused livers ... | 1982 | 6122362 |
| production of monoclonal antibodies by hybridomas sensitized to sporozoites of plasmodium berghei. | hybridoma cell lines, which secreted antibodies directed against either the surface of plasmodium berghei sporozoites or mosquito debris, were produced by fusion of spleen cells of p. berghei sporozoite-immunized mice with p3-x63-ag8 myeloma cells. four cloned antibody-secreting cell lines were successfully established. two of these clones (f9 and g10) were obtained from the fusion of spleen cells from mice that had undergone two immunizations. these clones produced an igm antibody that did not ... | 1982 | 6129292 |
| plasmodium berghei: immunologically active proteins on the sporozoite surface. | 1982 | 6174361 | |
| the use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei. | intraerythrocytic parasites of plasmodium vinckei and plasmodium berghei were separated according to their developmental stages using discontinuous percoll gradients. contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. the stages were unequivocally identified in smears using a newly developed dna-specific staining procedure with mithramycin and fluorescence microscopy. this stain can also be used to detect parasites in human blood of very low parasi ... | 1982 | 6177116 |
| [protective action of specific gamma globulin against malaria caused by plasmodium berghei]. | 1982 | 6179147 | |
| approaches to assessing host resistance. | there is increasing evidence that chronic, subclinical exposure to certain environmental pollutants may upset immune responsiveness and alter susceptibility of animals to infectious agents. environmental chemicals or drugs may affect diverse aspects of the immune system, leading to immunosuppression, immunopotentiation, hypersensitivity or perturbed innate host resistance. a variety of infectious models is available that involves relatively well defined target organs and host defense mechanisms; ... | 1982 | 6277617 |
| mechanism of diethyl-dithiocarbamate induced elevation of parasitemia in plasmodium berghei infection. | 1982 | 6282744 | |
| myristate-induced release of superoxide and hydrogen peroxide from peritoneal macrophages in mice immunized to toxoplasma gondii and plasmodium berghei. | 1982 | 6290731 | |
| synthesis and biological properties of 2'-deoxy-5-vinyluridine and 2'deoxy-5-vinylcytidine. | rapid and efficient syntheses for the preparation of 2'-deoxy-5-vinyluridine and 2'-deoxy-5-vinylcytidine are described starting from nucleoside precursors. contrary to some previous reports, 2-deoxy-5-vinyluridine has be found to be quite stable under normal laboratory conditions but when tested in animals shows neither toxicity nor anti-leukemic (l1210 cells) or anti-parasitic (plasmodium berghei) activity. 2'-deoxy-5-vinylcytidine appears to polymerise readily. it is much less toxic to cell c ... | 1982 | 6292837 |
| interactions between the intestinal flagellates giardia muris and spironucleus muris and the blood parasites babesia microti, plasmodium yoelii and plasmodium berghei in mice. | in mice infected with the intestinal flagellates giardia muris or spironucleus muris, together with the blood parasites babesia microti or plasmodium yoelii, there is a temporary decrease of flagellate cyst output coincident with the peak of the blood parasite infections, followed by a rapid return to normal levels. this decrease in cyst output is correlated with decreased numbers of trophozoites in the small intestine. the effect on s. muris is more marked than that on g. muris. neither blood p ... | 1982 | 6214756 |
| kinetics of immunosuppression of sporozoite-induced immunity by mycobacterium bovis bcg. | the data reported in this study demonstrate that the vaccination of nih/nmri mice with viable mycobacterium bovis bcg organisms induces a state of immunosuppression that renders the recipient animals incapable of a protective immune response to the malaria sporozoite vaccine. the expression of this altered protective immune response is dependent upon the dosage of the two live vaccines, as well as upon the sequence of their administration. data presented here show that the skin test responses (a ... | 1982 | 6215354 |
| chemiluminescence response of peritoneal macrophages to parasitized erythrocytes and lysed erythrocytes from plasmodium berghei-infected mice. | the chemiluminescence response of normal mouse peritoneal macrophages to parasitized erythrocytes isolated from mice 3 weeks after infection with plasmodium berghei was examined. only 4 of 12 animals showed positive responses, whereas 8 showed negative responses. photomicrographs revealed that only in chemiluminescence-positive animals were parasitized erythrocytes attached to or phagocytized by macrophages. when lysed parasitized-erythrocyte cell suspensions were added to the peritoneal macroph ... | 1982 | 6749686 |
| virulent p. berghei malaria: prolonged survival and decreased cerebral pathology in cell-dependent nude mice. | the course of lethal plasmodium berghei infection was examined in nu/+ and t cell-deficient nu/nu balb/c mice. a rapidly fatal neurologic syndrome, including ataxia, hemiparesis, and seizures, was seen in the nu/+ mice early in the infection, whereas this syndrome was absent in the nu/nu mice. the nu/nu mice also developed anemia more slowly, had lower levels of immune complexes and total igg, and had smaller decreases in serum c3 compared with the nu/+ mice. histopathologic examination of the b ... | 1982 | 6749988 |
| fansidar prophylaxis, therapy, and immune responses in rodent malaria (plasmodium berghei). | in order to determine the effect of fansidar on plasmodial infection in mice, outbred, adult, swiss-webster mice were treated with fansidar (20 mg sulfadoxine and 1 mg pyrimethamine/kg body weight) at various intervals before and/or after inoculation with blood stages of plasmodium berghei. drug therapy resulted in cure if it was given before the parasitemia rose to 53%. oral administration of fansidar was more effective in reducing or preventing parasitemia than intramuscular injection. fatal i ... | 1982 | 6750070 |
| antimalarials. 13. 5-alkoxy analogues of 4-methylprimaquine. | a series of nuclear and side-chain analogues of 4-methylprimaquine incorporating an alkoxy group in the 5-position of the quinoline nucleus has been prepared. the compounds were tested for suppressive antimalarial activity against plasmodium berghei in mice and for radical curative antimalarial activity against plasmodium cynomolgi in the rhesus monkey. although the toxicity problems characteristic of the 8-aminoquinolines were not overcome, several of the compounds, surprisingly, were highly ef ... | 1982 | 6750123 |
| the chemotherapy of rodent malaria xxxiii. the activity of chloroquine and related blood schizontocides and of some analogues in drug-induced pigment clumping. | 1982 | 6751248 | |
| plasmodium berghei: uptake and distribution of chloroquine in infected mouse erythrocytes. | 1982 | 6751843 | |
| inhibition by cyclosporin a of rodent malaria in vivo and human malaria in vitro. | the development and course of normally lethal parasitemias in mice inoculated intraperitoneally with erythrocytic stages of plasmodium yoelii or plasmodium berghei were markedly affected by treatment with the antilymphoid drug cyclosporin a (cs-a). when the first of four daily subcutaneous 25-mg/kg doses of cs-a was given at the time of parasite inoculation, patent infections failed to develop. if begun up to 5 days earlier, this same treatment regimen prolonged the prepatent period, attenuated ... | 1982 | 6752020 |
| plasmodium berghei malaria: blockage by immune complexes of macrophage receptors for opsonized plasmodia. | immune complexes produced in vitro by mixing immune serum and soluble plasmodium berghei antigens and immune complexes precipitated from serum of acutely infected rats blocked macrophage receptor sites for opsonized plasmodia. the immune complexes were precipitated from acute-phase serum, using polyethylene glycol, and their composition was determined by using a raji cell immunofluorescence assay. the immune complexes contained immunoglobulin g (igg), igm, and malarial antigens. these results in ... | 1982 | 6752023 |
| differential sensitivity in vivo of lethal and nonlethal malarial parasites to endotoxin-induced serum factor. | sera from mice infected with plasmodium yoelii or plasmodium berghei and given endotoxin contained nonspecific mediators which killed both species of parasite and tumor cells in vitro. the sera resembled tumor necrosis sera obtained from mice given macrophage-activating agents such as propionibacterium acnes (formerly designated corynebacterium acnes) or mycobacterium bovis bcg and then endotoxin. cytotoxicity developed parallel to parasite killing activity and indicated that macrophages were ac ... | 1982 | 6752029 |
| direct infection of hepatocytes by sporozoites of plasmodium berghei. | to identify the unknown liver cell type initially invaded by sporozoites of mammalian malaria, young rats were inoculated intravenously with large numbers of plasmodium berghei sporozoites obtained from infected anopheles stephensi mosquitoes. fine structural studies of liver specimens obtained from the rats within 2 min after inoculation demonstrated the presence of morphologically unaltered sporozoites in the cytoplasm of hepatocytes. many sporozoites were also observed undergoing cytolysis wi ... | 1982 | 6752394 |
| the demonstration of plasmodium berghei sporozoites in rat hepatocytes one hour after inoculation. | 1982 | 6753390 | |
| cellular changes in bone marrow of malaria-infected mice. iii. chemotaxis of granulocytes. | the number of bone marrow cells and their chemotactic activity was studied during malaria infection. two days after infection of balb/c mice with plasmodium berghei, an increase in granulocyte number was observed in the blood. a modified boyden chamber chemotaxis assay was employed to investigate the mechanism of granulocyte accumulation in the blood. bone marrow cells from normal mice, from mice during a primary lethal infection and from immune mice after challenge were compared. the complement ... | 1982 | 6753391 |
| 2-acetylpyridine thiosemicarbazones. 4. complexes with transition metals as antimalarial and antileukemic agents. | reaction of the 2-acetylpyridine thiosemicarbazones, 3-azabicyclo[3.2.2]nonane-3-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (iiia), its selenium analogue (iiib), 1h-hexahydroazepine-1-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (iv), and 1h-octahydroazocine-1-thiocarboxylic acid 2-[1-(2-pyridyl)ethylidene]hydrazide (v) with cu(ii), ni(ii), fe(iii), and mn(ii) salts gave crystalline complexes. relative to the free ligands, these complexes show reduced antimalarial activ ... | 1982 | 6754934 |
| susceptibility of plasmodium berghei in a laboratory population of anopheles atroparvus (diptera: culicidae) after the introduction of plasmodium-refractory genotypes. | 1982 | 6754936 | |
| ferriprotoporphyrin ix binding substances and the mode of action of chloroquine against malaria. | bovine serum albumin and preparations of cell sap from malaria parasites and normal erythrocytes were tested for ability to protect cellular membranes against the toxicity of ferriprotoporphyrin ix (fp) and a chloroquine-fp complex. suspensions of plasmodium berghei (approximately 7 x 10(6) parasites per ml, isolated from saponin-lysed, infected erythrocytes) were used as a test system. toxicity was monitored by measuring changes in turbidity of these suspensions at 700 nm. parasite cell sap (0. ... | 1982 | 6755119 |
| degenerating exo-erythrocytic forms of plasmodium berghei in rat liver: an ultrastructural and cytochemical study. | morphological and cytochemical aspects of the host response to almost mature exo-erythrocytic forms (eef) of plasmodium berghei in rat hepatocytes were studied by electron microscopy. young stages (less than 47 h) never evoked a local reaction. two types of nearly mature eef (47-51 h) could be distinguished, normal (eef type i) and those that were the target of infiltrating cells (eef type ii). the latter were in a stage of early or advanced degeneration and generally exhibited increased electro ... | 1982 | 6755366 |
| adoptive transfer of immunity to plasmodium berghei with spleen cells from drug cured mice. | plasmodium berghei infection gives rise to a fatal fulminating parasitaemia in mice. resistance to the infection can be achieved if the mice are allowed to recover from the blood-induced infection by administration of chloroquine. in cba/caj, one cycle and in balb/c mice, at least, two cycles of infection, alternating with drug-cure were necessary for the establishment of immunity. no parasitaemia was seen following further challenges after the third infection. adoptive transfer of spleen cells ... | 1982 | 6755741 |
| effect of multiple sporozoite challenges on immunity to malaria. | 1982 | 6755744 | |
| androgen suppression of circulating immune complexes and enhanced survival in murine malaria. | 1982 | 6757933 | |
| detection of exoerythrocytic stages of plasmodium berghei in fixed liver tissue and cultured cells by an immunoperoxidase antibody technique. | an immunoperoxidase antibody (ipa) technique is described for the detection of exoerythrocytic (ee) stages of plasmodium berghei in rat liver sections or cultured w138 cells. ee stages were detected in liver sections 12 hours after inoculation of sporozoites, but as early as three hours after the inoculation of w138 cells. by 42 hours in liver sections and 48 hours in w138 cells, ee parasites were easily visible by low power microscopy, and merozoites were clearly seen by 72 hours within ee para ... | 1982 | 6758221 |
| loss of infectivity for swiss mice of a mefloquine-resistant strain of plasmodium berghei. | a strain of plasmodium berghei made resistant to a 244 mg/kg daily dose of mefloquine by interrupted subcurative therapy in successive passages in weanling rats, was maintained for more than six months under drug pressure and was cryopreserved. experiments with this strain have shown its loss of virulence for intact mice. in splenectomized swiss mice infection with this resistant strain is transient. the mefloquine resistance of this line was unstable and the strain became sensitive to mefloquin ... | 1982 | 6758223 |
| [development of pyronaridine-resistance in plasmodium berghei]. | 1982 | 6758485 | |
| [synthesis of some 2, 6, 9-substituted thioguanine and their antimalarial activity]. | 1982 | 6758486 |