Publications

entamoeba histolytica and entamoeba invadens: effects of temperature and oxygen tension on growth and survival.		1980	6245905
[amebic liver abscess : value of extemporaneous search for ameba].		1980	6246317
hepatic amoebiasis in immunodepressed mice.	the effects of immunodepression on intestinal amoebic lesions produced by entamoeba histolytica were studied in inbred mice of the c3h/mg strain. immunodepression was induced in two ways by giving (i) cyclophosphamide and (ii) anti-mouse lymphocyte serum. on immunodepression with the latter, although there was no difference in the degree of macroscopic intestinal damage, all five surviving immunodepressed mice showed hepatic lesions. it is noted that these had a striking histological resemblance ...	1980	6247788
respiratory metabolism of giardia lamblia.		1980	6248623
lectin activity in entamoeba histolytica trophozoites.	a lectin (carbohydrate-binding protein) has been found in extracts of a number of axenically grown trophozoites of entamoeba histolytica strains. the strains grown in tyi-s-33 medium (diamond et al., trans. r. soc. trop. med. hyg. 72: 431-432, 1978) were hk-9, 200:nih, and hm-1:imss. strain hu-1:musc (hsc) was grown monoxenically in the same medium. the amoebic lectin agglutinated glutaraldehyde-fixed erythrocytes. this activity was ph dependent and heat and oxidation sensitive, and was destroye ...	1980	6249757
cytopathogenic mechanisms of entamoeba histolytica.	cinemicrography of entamoeba histolytica destruction of chinese hamster ovary (cho) cells shows that ameba cytopathogenicity consists of separate components: a contact-dependent cytolethal effect, and phagocytosis. cells not in contact with amebae remain intact. quantitation of ameba destruction of cho cells by applying the one-hit hypothesis confirms that the cytoethal effect of amebae is contact dependent. studies with 111indium oxine-labeled cells provide further evidence of extracellular kil ...	1980	6249882
plasma membrane of entamoeba histolytica.	axenically propagated entamoeba histolytica (hk9:nih strain) were employed as starting material for the isoation of plasma membrane by a novel procedure. in the absence of known enzymatic markers, the externally disposed polypeptides of intact amoebae were iodinated and the incorporated label used to monitor membrane separation and recovery. 12 major plasma membrane polypeptides (12 x 10(3)-200 x 10(3) mol wt) were labeled and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresi ...	1980	6249883
attachment and short-term maintenance of motility and viability of entamoeba histolytica in a defined medium.	organic requirements for attachment to glass, elongation, and motility of entamoeba histolytica, have been determined. the trophozoite, which has been grown axenically only in highly complex media with reduced oxygen tensions, remains rounded and detached when placed in a tris-hcl buffered solution containing nacl, kcl, mgcli, and cacl2. a maintenance medium in which the amebae could attach to glass, elongate, and remain motile and viable for 12 to 24 h was devised with the addition of cysteine, ...	1980	6249921
cell-mediated immune response in amebiasis.	studies on cell-mediated immune response (cmir) to amebia antigen in amebic liver abscess showed conflicting results. the leucocyte migration test was employed in nine cases of amebic liver abscess and cmir to amebic antigen was demonstrated in four cases. the role of cmir in the pathogenesis of amebic liver abscess remains unknown but its presence is confirmed.	1980	6250227
hepatic and thoracic amaebiasis.	sonographic and radiographic fidings were reviewed in 27 patients with hepatic amebiasis. sonography usually demonstrated nonspecific, peripheral, hypoechoic lesions. the only diagnostic sonographic appearance was a combination of a hypoechoic lesion and diaphragmatic disruption, which was found in four patients. about 50% of the patients had accompanying radiographic abnormalities that were nondiagnostic. these included elvation of the right hemidiaphragm, basilar pulmonary infiltrates, and ple ...	1980	6250386
application of thin layer immunoassay (tia) for demonstration of antibodies against entamoeba histolytica.	thin layer immunoassay (tia) was used to demonstrate antibodies against entamoeba histolytica in sera from patients and blood donors. the tia results agreed well with those obtained by the indirect hemagglutination (iha) and immunodiffusion (id) techniques. it is suggested that because of its technical simplicity and low cost tia may be used alternatively or in addition to the iha and id techniques for screening patient sera for antibodies to e. histolytica. however, the new technique must first ...	1980	6250415
activity of quinfamide against natural infections of entamoeba criceti in hamsters: a new potent agent for intestinal amoebiasis.	a novel tetrahydroquinolinyl ester, quinfamide, administered orally in multiple doses for 3 days had an ed50 of 0.25 mg/kg/day (total dose 0.75 mg/kg) for eradicating entamoeba criceti in hamsters in several tests. it was significantly more active by direct comparison than 3 commercially available amoebicides and at least as active as 2 other esters of the parent compound, 1-(dichloroacety)-1,2,3,4-tetrahydro-6-quinolinol. after administration of a single dose, ed50 calculations for quinfamide a ...	1980	6252530
cell mediated immune responses in experimental amebiasis.	cell migration was inhibited when entamoeba histolytica antigen-sensitized peritoneal exudate cells were cultured in the presence of specific antigen. the inhibition of cell migration was caused due to interaction of antigen with sensitized lymphocytes. this migration inhibition was well correlated with cell mediated immune responses, but not with circulating antibody levels. peritoneal cells obtained from animals immunized with 70.5 microgram of antigen gave a better response in capillary tube ...	1980	6252860
sequential histopathology of cavitary liver abscess. formation induced by axenically grown entamoeba histolytica.	multiple hamster liver passage of entamoeba histolytica trophozoites with intervening recovery into axenic culture caused increased virulence as measured by increase in the size of the lesion produced. lesions produced by amebae that had not been liver-passaged did not persist; however, multiply liver-passaged substrains produced large, fluid-filled abscesses one month to six weeks after inoculation. six days after inoculation, lesions consisted of multiple granulomas, lymphocytes, and e histoly ...	1980	6252861
entamoeba histolytica: cytopathogenicity, including serum effects on contact-dependent and toxin-induced lysis of hamster kidney cell monolayers.		1980	6253314
occurrence of hepatic amoebiasis in rats inoculated intracaecally with a virulent strain of entamoeba histolytica.		1980	6254213
immunogenicity of axenic entamoeba histolytica antigen and its fractions.	amoebic antigen prepared from axenic entamoeba histolytica (nih:200 strain) grown at 37 degrees c in tps i medium was chromatographed on a sephadex g-200 column. three fractions (f-i, f-ii, f-ii) were obtained, the molecular weights of which ranged from 650,000 to 1,450. fractions were concentrated by lyophilization and antisera against each fraction and whole antigen were raised in rabbits. partially purified fractions were used in various serological tests and their reactivities were compared ...	1980	6254215
amoebic antigen in immunodiagnosis and prognosis of amoebic liver abscess.	the detection of amoebic antigen by counterimmunoelectrophoresis is very useful and important in the immunodiagnosis of invasive hepatic amoebiasis. the antigen was demonstrated in 115 (92%) of 125 patients with amoebic liver abscess. all the 19 cases which showed entamoeba histolytica in the 'pus' were positive for the antigen and 96 of 106 samples negative for the parasite by smear and culture were also positive for the antigen. in none of the controls was a falsepositive reaction obtained. 12 ...	1980	6254216
experimental amoebic infection in guinea-pigs immunized with low grade amoebic infection.	guinea-pigs were immunzied with a low grade amoebic infection, either intracaecally or intramesenterically. the immunized animals were challenged with a virulent strain of entamoeba histolytica. some of the animals, immunized via the intracaecal route were challenged repeatedly. 10 to 30% of immunized animals developed caecal lesions compared with 92.8% of non-immunized animals. the percentage of protection in animals challenged repeatedly was greater than in those challenged once. neither immun ...	1980	6254217
comparison of iha test for amoebiasis on serum and filter paper specimens.		1980	6254220
protection against amebic liver abscess in hamsters by means of immunization with amebic antigen and some of its fractions.	immunization of hamsters by intramuscular injections of an amebic extract and of two of its fractions, emulsified with complete freund's adjuvant, resulted in protection against the development of amebic liver abscess, following intrahepatic inoculation of axenic trophozoites of entamoeba histolytica such that fraction i, fraction ii, and the whole antigen conferred protection to 18 out of 18, 4 out of 18, and 11 out of 18 hamsters, respectively. splenomegaly was found to accompany the developme ...	1980	6254382
surface coagglutination with formalinized, stained protein a staphylococci in the immunologic study of three pathogenic amebae.	the indirect immunofluorescence and, to a lesser extent, the immunoperoxidase methods have become the principal means of conducting immunological studies upon amebae. these procedures require the conjugation of fluorescein or enzyme with antiglobulin reagents and fixation of the amebae. the present report describes a somewhat similar methodology wherein formalinized, stained protein a staphylococci combined with specific antibody have been found to coagglutinate upon a portion of the amebic surf ...	1980	6254383
mitogenicity of entamoeba histolytica extracts for murine lymphocytes.	aqueous extracts or aqueous extracts of delipidated entamoeba histolytica (e.h.e.) contain a mitogenic principle for murine lymphocytes. as detected by [3h]-thymidine incorporation and blast transformation, e.h.e. acted predominantly on t cells of splenic origin, but not on thymocytes or bone marrow cells. furthermore, e.h.e. induced proliferation of a subset of non-t-cells which is present in the spleen of athymic nude mice, adhered to nylon wool, but could not be activated to produce antibody. ...	1980	6254869
a comparative study of entamoeba histolytica (nih :200, hk9, etc.), "e. histolytica-like" and other morphologically identical amoebae using isoenzyme electrophoresis.	cultures of old, well documented strains of entamoeba histolytica (nih:200, hk9 etc.) have been compared with "e. histolytica-like" strains and, in turn, with e. moshkovskii, e. invadens and e. chattoni. they have been grouped according to their isoenzyme patterns. the "e. histolytica-like" organisms give patterns similar to e. moshkovskii and both of these are different from e. histolytica groups i to iv. e. invadens and e. chattoni have easily distinguished characteristic isoenzyme bands. "e. ...	1980	6255635
protective effects of crude and chromatographic fractions of axenic entamoeba histolytica in guinea-pigs.	axenic amoebic antigen was chromatographed on sephadex g-200. two peaks divided into three fractions were obtained which were concentrated by lyophilization. guinea-pigs were given a series of subcutaneous injections of crude entamoeba histolytica antigen and its chromatographic fractions mixed with freund's complete adjuvant. after three immunizing doses, the animals were challenged with intracaecal injection of a virulent isolate of amoebae (strain no. h-25) and were killed seven days later. t ...	1980	6255636
niacin requirement for growth of axenic entamoeba histolytica.	niacin (nicotinic acid) was found to be essential for the cultivation of axenic entamoeba histolytica. this vitamin requirement was also satisfied by nicotinamide. panmede liver digest, a source of vitamins in the axenic medium was replaced with a dialyzed hot water extract of homogenized whole liver, supplemented with various growth factors. cultured medium made with the liver extract and not supplemented with niacin failed to support continued multiplication of e. histolytica, but did support ...	1980	6255809
an immunofluorescence test to detect serum antibodies to giardia lamblia.	we used an indirect immunofluorescence test with giardia lamblia trophozoites as antigen to detect anti-g. lamblia antibodies in serum. seventy-one patients and control subjects were studied in a blinded protocol. titers in 29 of 30 patients with symptomatic giardiasis (1:16 to 1:1024) did not overlap titers in 19 healthy control subjects (1:2 to 1:4); titers in 15 patients with hookworm, entamoeba histolytica, or intestinal bacterial overgrowth were 1:16 or less absorption of giardiasis patient ...	1980	6255845
[do hepatotropic strains of entamoeba histolytica exist?].	liver amoebic abscess often occur without any colitic symptoms nor any parasite detection in the colon. the localisation heterogeneity and the experimental data, associated with clinical expression suggest the existence, inside the species e. histolytica, of various strains: non pathogenic one, invasive for the intestine mucous membrane ones and invasive for liver tissue ones.	1980	6257412
studies on the examination of imported laboratory monkey, macaca fascicularis for e. histolytica and other intestinal parasites.	infection rate of imported monkeys, under this study, with intestinal protozoa is higher than the infection rate with intestinal nematodes. mostly encountered protozoa were e. coli, e. nana, e. histolytica and i. butschlii. formalin-ether concentration method yielded higher % positive for e. histolytica than the culture method using tanabe-chiba medium, but the results obtained from these 2 methods correlate perfectly well with each other. efficacy of thiabendazole against intestinal nematodes s ...	1980	6257621
incorporation of glucose carbon into ribonucleotides of axenic entamoeba histolytica.	axenic entamoeba histolytica grown with (ul-14c)glucose in tp-s-1 medium were capable of biosynthesizing ribose from labeled glucose. rna isolated by phenol extraction was hydrolyzed to the ribonucleotide level by alkaline hydrolysis. the hydrolysate, chromatographed on ion exchange resins, yielded amp, gmp, and ump, but not cmp containing labeled glucose carbon. the present nucleotide composition of the isolated amebal rna was, respectively, as follows, cmp, 0.20; gmp, 0.22; amp, 0.30; ump, 0.2 ...	1980	6257878
purification and properties of nadph:flavin oxidoreductase from entamoeba histolytica.	amebal nadph:flavin oxidoreductase was purified to apparent homogeneity. molecular weights of 40 000 and 38 000 were estimated by gel filtration and by sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, indicating that the enzyme is composed of a single polypeptide chain. the enzyme does not contain firmly bound flavin. it exhibited 20-fold selectivity for nadph over nadh. with the former donor it reduced riboflavin, galactoflavin, fmn, or fad. aerobically the reducing equi ...	1980	6258069
dynamics of the interaction between entamoeba histolytica and components of the immune response.	trophozoites of entamoeba histolytica redistributed specific antibodies reacting to antigens on their surface. the presence of a second layer of anti-antibodies enhanced the redistribution of anti-amoeba antibodies but was not a prerequisite for this process. microfluorometric measurements of the fluorescence intensity in different areas of the cell surface provided objective evidence for the antibody-induced redistribution. in order to distinguish surface-bound and internalized anti-amoeba anti ...	1980	6258222
protozoal infection in human beings in india. a critical review of the literature.		1980	6258497
a lectin activity in entamoeba histolytica trophozoites.	a lectin (carbohydrate binding protein) has been found in extracts of a number of axenically grown trophozoites of entamoeba histolytica strains. the strains grown in diamond's tyi-s-33 media were hk-9, 200:nih and hm-1: imss. strain hu-1: musc (hsc) was grown monoxenically in the same medium. the amoeba lectin agglutinates glutaraldehyde-fixed red blood cells. this activity is ph dependent, heat and oxidation sensitive and is destroyed by proteolysis upon auto-incubation. the relative agglutina ...	1980	6258503
serum proteins in the plasma membrane of entamoeba histolytica.	the ability of axenic entamoeba histolytica, strain 200:nih, to adsorb serum proteins was studied. amebae were grown in diamond's tps-1 medium. serological reactivity was observed between the plasma membrane of e. histolytica and rabbit anti-bovine or anti-human serum when the amebae were grown, respectively, with bovine or human serum. antisera were obtained from rabbits immunized with culture medium, amebal homogenates, and various amebal subcellular fractions: soluble phase, microsomal fracti ...	1980	6258504
studies on the cytopathogenicity of entamoeba histolytica.	we have explored the interaction of virulent and avirulent e. histolytica (hm1 and 303 respectively) with human polymorphonuclear leukocytes (pmns) and chinese hamster ovary (cho) cells. pmns exhibit chemotaxis to both hml and 303. on contact with hml, pmns degranulate and die (trypan blue uptake); incubation at a ratio of 100 pmns per ameba for 40 minutes revealed 70 per cent pmn death with 84 per cent hml viability. 303s are often ingested by pmns on contact; after incubation as above, only 1 ...	1980	6258505
further studies on a cytotoxin/enterotoxin from entamoeba histolytica.	more virulent strains of entamoeba histolytica contain more cytotoxic activity per trophozoite than do less virulent strains. clarified sonicates of the more virulent strains are more cytotoxic than those of less virulent strains. the cytotoxins of all these strains are inhibited by specific immune igg raised in rabbits or produced by natural human infection. non-immune igg's had no antitoxic activity. serum fractions of non-immune serum containing alpha globulins (alpha 2 macroglobulin and alph ...	1980	6258506
serum-inhibited toxicity of entamoeba histolytica.	the cytopathogenic effect (cpd) of soluble antigens prepared from various strains of entamoeba histolytica, differing in virulence, was assayed on monolayers of baby hamster kidney cells growing in microtiter plates. serum-inhibited toxicity could be detected in all these strains and in e. invadens, but not in the huff strain and in an acanthamoeba species. gel chromatography demonstrated a peak activity of the toxin in a fraction with m.w. between 35,000 and 45,000. other experiments learned th ...	1980	6258507
detection of antibodies against lectin-like "toxin" of entamoeba histolytica in sera of patients with invasive amebiasis.		1980	6258508
[effect of entamoeba histolytica on the chemotaxis of human monocytes].		1980	6258509
[virulence and surface properties of various axenic strains of entamoeba histolytica].		1980	6258510
[specific proteins and culture media proteins found in trophozoites of e. histolytica obtained by axenic cultivation].		1980	6258511
sequential histopathology of cavitary liver abscess formation induced by axenically grown entamoeba histolytica.	animal models of amebic hepatic abscess should parallel the human disease. experimental amebic hepatic abscess in the hamster results in granulomatous lesions during the early period (six days) following inoculation. hamster inoculated intrahepatically with axenically grown, non-liver passaged strains of entamoeba histolytica revealed no evidence of lesions when sacrificed at later times. however, hamsters similarly inoculated with multiply liver passaged substrains were found to have large flui ...	1980	6258512
[development of lesions produced in hamsters by inoculation of entamoeba histolytica].		1980	6258513
[histochemistry of the hepatic lesion produced in hamsters by inoculation with e. histolytica from axenic cultures].		1980	6258514
[participation of t lymphocytes in the anti-entamoeba histolytica immune response in hamsters].		1980	6258515
[decreased activity of the mononuclear phagocytic system in hamsters infected with entamoeba histolytica].		1980	6258516
loss of susceptibility to antibody and complement mediated lysis in e. histolytica.		1980	6258517
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. i. introduction].		1980	6258518
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of e. histolytica. ii. isolation and characteristics of lysosomal antigen].		1980	6258519
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. iii. counterimmunoelectrophoresis and indirect hemagglutination reactions in monkey serum].		1980	6258520
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. iv. cytopathogenic effect of monkey serum on trophozoites of entamoeba histolytica].		1980	6258521
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. v. intradermal reactions with histolyticin in monkeys].		1980	6258522
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. vi. intrahepatic inoculation of virulent e. histolytica in monkeys].		1980	6258523
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. vii. comments and conclusions].		1980	6258524
[diagnostic and therapeutic evaluation of invasive intestinal amebiasis in children].		1980	6258525
isolation of the plasma membrane of entamoeba histolytica.		1980	6258526
axenic cultivation of entamoeba histolytica: progress and problems.	eighteen years of technological progress in the field of axenic cultivation of entamoeba histolytica is reviewed. during this period cumbersome diphasic media gave way to easily prepared clear liquid media. yields of amebae were increased a thousand fold, and doubling time reduced from 24 to less than 8 hours. attention is directed to three problems, any one of whose solution will extend the potencial of the axenic cultures. they are 1) to develop a defined medium, 2) to reduce the interval betw ...	1980	6258527
dynamic changes on the surface of entamoeba induced by antibodies.	antibody and concanavalin a bound to the cell surface of entamoeba induced segregation of these complexes towards the uroid, followed by an evagination of the membrane portion that contained the components redistributed. this vesiculated cap was released into the medium. con a polarized all con a-binding glycoproteins from the surface of the ameba while with antibodies only some of the surface antigens were movilized towards the cap area. this phenomenon was temperature dependent as shown in e. ...	1980	6258528
role of iron-sulfur proteins in the electron transport system of entamoeba histolytica.		1980	6258529
purine nucleotide synthesis in entamoeba histolytica: a preliminary study.	entamoeba histolytica, axenically grown, were unable to incorporate 14c-glycine into extractable purines, even after 16 hours incubation in the presence of this compound. similarly, amoebas did not incorporate 14c-sodium formate into purines. such data indicate a total absence of a de novo purine biosynthetic pathway in this amoeba. when amoebas were incubated in the presence of 3h-adenine and 3h-adenosine for one hour, considerable radioactivity was associated with amp, adp and atp; no apprecia ...	1980	6258530
[transfer ribonucleic acids of entamoeba histolytica. i. chromatographic analysis of the trna isoacceptor species of trophozoites].		1980	6258531
physico-chemical factors affecting the behaviour and activity of entamoeba histolytica.	activity of individual trophozoites of several strains of axenic entamoeba histolytica under different environmental conditions was measured by means of a newly devised method. activity was found to be different between strains and corresponded to the degree of their pathogenicity. at low ph values amoebae moved further due to a straightening of their paths. a similar phenomenon was found in 1:50 cilution of pig bile. bile was also found to induce an increase in the number of monopodial, "limax- ...	1980	6258532
comparison of conventional and immunofluorescent techniques for the detection of entamoeba histolytica in rectal biopsies.	two comparative studies of six staining techniques in detection of entamoeba histolytica in rectal biopsies were performed. the techniques evaluated were direct and indirect fluorescent antibody technique, and four conventional stains: pas, h & e, trichrome, and ptah). on ethanol-fixed tissue, the direct fluorescent antibody technique was the most sensitive, detecting amoebas in 84% of rectal biopsies from 19 aspirate-positive dysentery patients. pas staining was specific, almost as sensitive, a ...	1980	6153164
entamoeba histolytica "toxin": fetuin neutralizable and lectin-like.	extracts of entamoeba histolytica produce rounding and agglutination of mammalian cells in tissue culture. this effect is inhibited or reversed by serum, by the serum glycoprotein, fetuin, or by n-acetyl-d-galactosamine. concanavalin a produces a similar cytotoxic effect that is blocked or reversed by alpha-methyl mannoside. these properties suggest that the amebal "toxin" has a lectin-like activity.	1980	6153255
parasite-reactive serum ige antibodies in african populations. relation to intestinal parasite load.	serum ige levels and ige antibodies against four intestinal parasites, and the presence and abundance of parasites in stool samples were investigated in 161 rwanda natives. most of the ige turn out to be helminth-reacting antibodies. the stimulation and production of these antibodies are much different from those of anti-amoeba antibodies in their relation to the specific intestinal parasite load; helminth infestations seem to play a major role in the development of anti-amoeba antibodies as wel ...	1980	6154649
antigenic variations among strains of entamoeba histolytica.		1980	6156662
purification of the antigen specific for amoebiasis.	two methods used for the purification of specific antigen for amoebiasis were affinity chromatography and agarose gel electrophoresis. the igg fraction of the serum from a patient with amoebic liver abscess showing a specific band in the iep test was coupled to cnbr-activated sepharose 4b. after passage through the immunoadsorbent column of the crude or partially purified extract of entamoeba histolytica, followed by acid dissociation, specific antigen was obtained. this antigen gave only one ba ...	1980	6160623
[staining of cysts of entamoeba invadens, entamoeba histolytica and entamoeba coli with wheat germ agglutinin labelled with colloidal gold].	cysts of entamoeba invadens obtained under axenic conditions were stained with wheat germ agglutinin labelled with colloidal gold. microscopic observation of encysting cultures revealed that upon staining, cysts acquired a red coloration, while trophozoites remained unstained. e. histolytica and e. coli cysts obtained from asymptomatic patients were also stained red by this technique. electronmicroscopic examination of stained cells showed a layer of colloidal gold granules attached to the surfa ...	1980	6162428
concepts of function of peripheral non-chromatin and endosome in entamoeba histolytica.	the distribution of rna (to a lesser extent dna) in the nucleus of e. histolytica appeared to be the opposite of what one found in other eukaryotic cells (originally stated by pan and geiman in 1955). autoradiographs of amebae labelled with 3h-thymidine revealed primarily randomly distributed dna-3h (probably euchromatin) in interphase cells. this dna was so low in amount in individual cells that it usually did not stain with the feulgen procedure. a small amount of dna-3h was also detected by a ...	1980	6162429
intestinal parasites in homosexual men.	a study on the prevalence of intestinal parasites in a group of homosexual men, attending a sexually-transmitted diseases clinic in glasgow, was undertaken. of 118 men examined over an eight-month period, four, one of whom had symptoms of dysentery, were found to be infected with entamoeba histolytica. cysts of iodamoeba buetschlii were also found in the stool of one of these men. a further two patients had giardiasis, and 11 men had enterobiasis. the importance of an awareness of these conditio ...	1980	6259724
specific anti-amoebic immunoglobulins and the cellulose acetate precipitin test in entamoeba histolytica infection.	using the indirect fluorescent antibody test and sera from 10 proved cases of invasive amoebiasis, the effects of absorption of igg on observed titres of amoeba-specific igm and iga were investigated. in addition, results of cellulose acetate precipitin tests were compared with anti-amoebic antibody levels. antiamoebic igm was found at titres of 1:14 to 1:112 after igg absorption in four cases. anti-amoebic iga was detected in sera from four cases, but the maximum titre was 1:28. there was no re ...	1980	6259778
the epidemiology of entamoeba histolytica in mexico city. a pilot survey i.	stocks of intestinal amoebae isolated from hospital patients in mexico city and grown in monoxenic culture were compared among themselves and with those already described (sargeaunt & williams, 1979), using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm), l-malate:nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk). new isoenzyme groups (sargeaunt & williams, 1979) of all the amoebae, including entamoeba histolyti ...	1980	6259779
electrophoretic studies of the hexokinase of entamoeba histolytica groups i to iv.		1980	6259781
study of the trophozoite surface of entamoeba histolytica with high resolution cytochemical studies and scanning electron microscopy.	a study of positive and negative electrical surface charges of entamoeba histolytica trophozoites axenically cultured was carried out by means of incubation with colloidal iron solutions at ph 4.0 and 1.8, respectively. the samples were examined under the transmission electron microscope. human erythrocytes were studied using the same experimental conditions as controls. in these cells the surface negative charges were homogeneously distributed on the surface, whereas the positive charges were o ...	1980	6260049
[an evaluation of the therapeutic action of tinidazole suppositories in intestinal infections by protozoa in children (author's transl)].		1980	6260126
attachment of entamoeba histolytica to glass in a defined maintenance medium: specific requirement for cysteine and ascorbic acid.	cysteine and ascorbic acid were previously shown to be required by entamoeba histolytica trophozoites for attachment to glass, elongation, and ameboid movement as well as for short-term (12-24 h) survival in a balanced salt solution containing bovine serum albumin and a vitamin solution (maintenance medium 1). if the only function of cysteine and ascorbate was to decrease the redox potential, other reducing agents should be effective. however, the requirement for cysteine in the presence of asco ...	1980	6260930
[chronic infiltration of the cecum and appendix in entamoeba histolytica infection].		1980	6261462
[cervico-uterine amebiasis. characteristics of 15 cases and review of the literature].		1980	6270758
[effects of prostaglandins (pge1) on the development of amebas i experimentally infected rats].		1980	6267999
[experimental amebiasis: effect of pge1 on rats infested with entamoeba histolytica].	the authors have studied the effects of pge1 on rats (wistar strain) infected with entamoeba histolytica, and inoculated with pge1 either in massive dosage and killed after 3-5 and 7 days, or with daily minimum quantity of pge1 and killed on days 7 and 15 after inoculation. the results show an inhibition effect of pge1 on development and survival of amoebae in the coecum of rats inoculated with the two modalities described above. the fractionated dosage gives better results and less side effects ...	1980	6268000
amebae resembling entamoeba gingivalis in the genital tracts of iud users.	a total of 2,252 vaginopancervical (fast) smears were studied as a continuation of an ongoing program to further investigate the occurrence of amebae in the genital tracts of women using intrauterine devices (iuds). of the 947 iud users, 1% harbored trophic amebae. no amebae, however, were found in any of the 1,164 smears from non-iud wearers. in all instances the amebae were associated with actinomyces. both organisms disappeared promptly after removal of the iud. in preparations stained accord ...	1980	6933802
amoebic liver abscess: clinical presentation and diagnosis.	the clinical profiles of 16 patients with amoebic liver abscesses referred to royal perth hospital over a ten-year period were assessed. the results indicate that amoebic abscess should be suspected in asian migrants and farmers, or patients who have travelled in endemic areas, who present with fever, anorexia, weight loss and abdominal pain. localized right hypochondriac tenderness, hepatomegaly and chest signs may be exhibited. the presence of anaemia, leucocytosis and marked elevation of the ...	1980	6934768
a seroepidemiological study of amebiasis in surinam by the enzyme-linked immunosorbent assay (elisa).	sera from nine populations in surinam, south america, were screened for antibodies against entamoeba histolytica by the enzyme-linked immunosorbent assay (elisa) technique and a precipitin test (gel diffusion or counterimmunoelectrophoresis). in two small surveys in 1974 and 1978, a high rate of clinically significant elisa titers was found in a rather isolated group of amerindians living near the brazilian border, predominately in the youngest age groups. amerindians living near the coast showe ...	1980	6992607
an ecological interdependence of diet and disease? a study of infection in one tribe consuming two different diets.	the nature and incidence of infections were studied in two groups of turkana living in the same area but eating different diets; one consumed milk only and the other a combination of fish and milk. the only apparent and significant nutritional difference between the two groups was mild iron deficiency in the milk drinkers. episodes of fever, symptomatic infection with malaria and brucellosis, molluscum contagiosum and common warts, episodes of diarrhea, and serological evidence of infection with ...	1980	7355850
antiamebic amidines and sulfonamides of 5- and 6-amino-2,3-bis(4-alkyl-1-piperazinyl)quinoxalines.	a series of amidines and sulfonamides of 5- and 6-amino-2,3-bis(4-alkyl-1-piperazinyl)quinoxalines was synthesized and tested against cecal and hepatic forms of entamoeba histolytica infections in rats and hamsters, respectively. four compounds (5, 6, 8, and 9) were found to have acceptable activity against infections in both species but were too toxic to be considered for use in man.	1980	7359534
extraintestinal amebiasis in infancy: report of three patients and epidemiologic investigations of their families.	three infants (aged 6 weeks, 7 weeks, and 10 months) had severe entamoeba histolytica infections characterized by colitis, hepatic abscesses, and peritonitis. the two younger children died after fulminant illnesses while the third recovered. diagnosis was delayed in all three children by a low index of suspicion and negative stool examinations for parasites. epidemiologic investigations of the infants' families revealed a high prevalence of amebic infections and elevated antibody titers to e his ...	1980	7367088
cytopathogenicity of entamoeba histolytica: hemolytic activity of trophozoite homogenates.	in in vitro assays the hemolytic activity of total homogenates of trophozoites of seven strains of amebae were tested for specificity and potency against the erythrocytes of eight mammalian species. the species of entamoeba included in the tests were e. histolytica, e. invadens, e. moshkovskii, and the laredo type of e. histolytica. the hemolytic activity was found to be dose-dependent and strain-specific, with e. histolytica being generally the species having the highest hemolytic potency.	1980	7369439
amoebic abscess in the cirrhotic liver.	though amoebic liver abscess and liver cirrhosis occur very commonly in hospital practice in the tropics, they have not to the knowledge of the present authors hitherto been reported to occur simultaneously in the same patient. the patient described here, who had clear-cut clinical and histological features of chronic liver cirrhosis with portal hypertension and ascites, presented somewhat acutely with liver pain and an amoebic liver abscess that contained 'chocolate sauce' on needle aspiration. ...	1980	7380340
shigellosis in the marshall islands: epidemiologic aspects of an outbreak.	an outbreak of diarrheal illness caused by shigella flexneri 1b and associated with 11 deaths occurred in the marshall islands during june and july 1977. a house-to-house survey on majuro indicated an attack rate of 6.2%. neither the survey nor a case-control study uncovered a common source of exposure on majuro, and it is believed that transmission was mainly person-to-person. socioeconomic factors, type of water supply, distance to municipal water supply, and type of sanitary facility could no ...	1980	7386724
the salutary effect of milk on amoebiasis and its reversal by iron.	observations among milk-drinking african nomads showed an unusual freedom from infection with entamoeba histolytica compared with similar nomads taking a mixed diet. a controlled study among maasai pastoralists showed that the administration of iron to correct their dietary iron deficiency sharply increased their susceptibility to amoebiasis. examination of the milk of their zebu cattle showed that it not only had a concentration of iron below the minimum necessary for the growth of e histolytic ...	1980	7388537
[frequency and significance of parasitic infections in refugees from vietnam (author's transl)].	in the course of the medical care which is provided for the refugees from southeast asia 100 persons were tested for intestinal parasites (helminths and protozoa). in addition serum samples were obtained from 72 of these and tested in several tests (cft, fat, iha and sft) using antigens of entamoeba histolytica, leishmania donovani, toxoplasma gondii, schistosoma spp., fasciola hepatica, echinococcus spp., trichinella spiralis and litomosoides carinii. in 49 persons infections with one, two or ...	1980	7395245
lysosomal enzymes in axenically grown entamoeba histolytica nih-200 passaged through cholesterol.		1980	7399589
intestinal parasitic infections in homosexual men: prevalence, symptoms and factors in transmission.	in a controlled study 67.5% of 200 homosexual men but only 16% of 100 heterosexual men were found to be infected with intestinal parasites. entamoeba histolytica was isolated from 27% of the homosexual and 1% of the heterosexual men, and giardia lamblia was isolated from 13% of the homosexual and 3% of the heterosexual men. the presence of symptoms could not be correlated with infection except when the infection was caused by more than one organism, including g. lamblia. symptoms were much more ...	1980	7437971
human parasitoses in bali, indonesia.	a survey of intestinal and blood parasites of man was conducted on the island of bali, indonesia in 1974. a total of 270 stool specimens from three villages were examined. both ascaris lumbricoides and trichuris trichiura were found in more than 90% of those examined, and hookworm eggs in 59%. other intestinal helminth and protozoan parasites found were: enterobius vermicularis, entamoeba histolytica, entamoeba coli, endolimax nana, and iodamoeba bütschlii. taeniid proglottids, hymenolepis dimin ...	1980	7444572
human intestinal parasites in karakuak, west flores, indonesia and the effect of treatment with mebendazole and pyrantel pamoate.	a survey for intestinal parasites and mass-treatment with a combination of mebendazole and pyrantel pamoate were conducted in karakuak, west flores in 1977. a total of 198 stool specimens from 104 males and 94 females ranging in age from less than 1 to 70 years were examined and 72% harbored one or more intestinal parasites. ascaris lumbricoides (43%) and entamoeba histolytica (21%) were the most common, followed by entamoeba coli (19%), hookworm (18%), iodamoeba bütschlii (8%), giardia lamblia ...	1980	7444573
entamoeba histolytica infection in male homosexuals.	amoebic infection in two male homosexuals is described. the possibility that this infection was acquired through homosexual practices and the implications to clinical and diagnostic services is discussed.	1980	7461471
[cellular behavior of axenic amebas cultured in the presence of different carbohydrates].	carbohydrates are an essential requirement of the culture medium in many cells. axenic cultures of amebas require a concentration of 0.5 to 1.0 per cent glucose. the purpose of this work is to demonstrate possible differences on the carbohydrates requirements between pathogenic and nonpathogenic amebas strains. the experimental work was done in two phases: a) observation of the cultures growing at different concentrations of glucose in tp-s-1 medium (0, 0.25, 0.50, 1.0 and 2.0 g/100 ml), and b) ...	1980	7469636
effect of complement depletion on the induction of amebic liver abscess in the hamster.	complement depletion in the hamster by cobra venom factor (cof) gives a higher frequency and severity of lesions after intrahepatic inoculation of entamoeba histolytica. the cof-treatment does not alter the humoral immune response to amebae, indicating that its effect on the amebic liver abscess is not due to suppression of the immune response but rather to its effect on complement levels at the time of the protozoal infection.	1980	7469638
[sequential study of the invasion of the colonic mucosa by entamoeba histolytica].		1980	7469640