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cotton (gossypium hirsutum l.).cotton continues to be a crop of great economic importance in many developing and some developed countries. cotton plants expressing the bt gene to deter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., china, india, and the usa. considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers several target traits that can be improved through genet ...201525416245
ghdrin1, a novel drought-induced gene of upland cotton (gossypium hirsutum l.) confers abiotic and biotic stress tolerance in transgenic tobacco.a novel stress tolerance cdna fragment encoding ghdrin1 protein was identified and its regulation was studied in cotton boll tissues and seedlings subjected to various biotic and abiotic stresses. phylogenetic and conserved domain prediction indicated that ghdrin1 was annotated with a hypothetical protein of unknown function. subcellular localization showed that ghdrin1 is localized in the chloroplasts. the promoter sequence was isolated and subjected to in silico study. various cis-acting eleme ...201525413882
a r2r3-myb transcription factor that is specifically expressed in cotton (gossypium hirsutum) fibers affects secondary cell wall biosynthesis and deposition in transgenic arabidopsis.secondary cell wall (scw) is an important industrial raw material for pulping, papermaking, construction, lumbering, textiles and potentially for biofuel production. the process of scw thickening of cotton fibers lays down the cellulose that will constitute the bulk (up to 96%) of the fiber at maturity. in this study, a gene encoding a myb-domain protein was identified in cotton (gossypium hirsutum) and designated as ghmybl1. quantitative real-time polymerase chain reaction (rt-pcr) analysis rev ...201525534543
silicon (si) alleviates cotton (gossypium hirsutum l.) from zinc (zn) toxicity stress by limiting zn uptake and oxidative damage.silicon (si) is as an important fertilizer element, which has been found effective in enhancing plant tolerance to variety of biotic and a-biotic stresses. this study investigates the si potential to alleviate zinc (zn) toxicity stress in cotton (gossypium hirsutum l.). cotton plants were grown in hydroponics and exposed to different zn concentration, 0, 25, and 50 μm, alone and/or in combination with 1 mm si. incremental zn concentration in growth media instigated the cellular oxidative damage ...201525516248
a comparative meta-analysis of qtl between intraspecific gossypium hirsutum and interspecific g. hirsutum × g. barbadense populations.based on 1075 and 1059 qtl from intraspecific upland and interspecific upland × pima populations, respectively, the identification of qtl clusters and hotspots provides a useful resource for cotton breeding. mapping of quantitative trait loci (qtl) is a pre-requisite of marker-assisted selection for crop yield and quality. recent meta-analysis of qtl in tetraploid cotton (gossypium spp.) has identified regions of the genome with high concentrations of qtl for various traits called clusters and s ...201525501533
the complexity of posttranscriptional small rna regulatory networks revealed by in silico analysis of gossypium arboreum l. leaf, flower and boll small regulatory rnas.micrornas (mirnas) and secondary small interfering rnas (principally phased sirnas or trans-acting sirnas) are two distinct subfamilies of small rnas (srnas) that are emerging as key regulators of posttranscriptional gene expression in plants. both mirnas and secondary-sirnas (sec-sirnas) are processed from longer rna precursors by dicer-like proteins (dcls). gossypium arboreum l., also known as tree cotton or asian cotton, is a diploid, possibly ancestral relative of tetraploid gossypium hirsut ...201526070200
a new synthetic amphiploid (aaddaa) between gossypium hirsutum and g. arboreum lays the foundation for transferring resistances to verticillium and drought.gossypium arboreum, a cultivated cotton species (2n = 26, aa) native to asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. however, it is quite difficult to transfer favorable traits into upland cotton through conventional methods due to the cross-incompatibility of g. hirsutum (2n = 52, aadd) and g. arboreum. here, we improved an embryo rescue technique to overcome the ...201526061996
itraq-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.).male sterility is a common phenomenon in flowering plants, and it has been successfully developed in several crops by taking advantage of heterosis. cotton (gossypium hirsutum l.) is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to use ccri9106 in cotton hybrid breeding, it is of great importance t ...201526047712
genetic fine mapping and candidate gene analysis of the gossypium hirsutum ligon lintless-1 (li1) mutant on chromosome 22(d).ligon lintless-1 (li1) is a gossypium hirsutum mutant that is controlled by a dominant gene that arrests the development of cotton fiber after anthesis. two f2 mapping populations were developed from mutant (li1 × h7124) f1 plants in 2012 and 2013; each was composed of 142 and 1024 plants, respectively. using these populations, li1 was mapped to a 0.3-cm region in which nine single-strand conformation polymorphism markers co-segregated with the li1 locus. in the published g. raimondii genome, th ...201526037218
mapping-by-sequencing of ligon-lintless-1 (li 1 ) reveals a cluster of neighboring genes with correlated expression in developing fibers of upland cotton (gossypium hirsutum l.).mapping-by-sequencing and snp marker analysis were used to fine map the ligon-lintless-1 ( li 1 ) short fiber mutation in tetraploid cotton to a 255-kb region that contains 16 annotated proteins. the ligon-lintless-1 (li 1 ) mutant of cotton (gossypium hirsutum l.) has been studied as a model for cotton fiber development since its identification in 1929; however, the causative mutation has not been identified yet. here we report the fine genetic mapping of the mutation to a 255-kb region that co ...201526021293
inheritance and qtl mapping of leaf nutrient concentration in a cotton inter-specific derived ril population.developing and deploying cotton cultivars with high nutrient uptake, use efficiency and tolerance to nutrient related soil stresses is desirable to assist sustainable soil management. genetic variation, heritability, selection response and quantitative trait loci (qtls) were investigated for five macronutrients (p, k, ca, mg, s) and five micronutrients (fe, mn, b, zn, and cu) in a recombinant inbred line (ril) population from an inter-specific cross between gossypium hirsutum cv. guazuncho 2, an ...201526020945
cloning and expression analysis of hsp70 gene from mangrove plant kandelia obovata under cold stress.heat shock protein 70 (hsp70), the primary member of the hsps that play various stress-protective roles in plants. in this study, a hsp70 gene of kandelia obovata (kohsp70) was cloned by rapid amplification of cdna ends (race). the full-length of kohsp70 was 2255 bp, consisting of a 5'-terminal untranslated region (utr) of 118 bp, a 3'-terminal utr of 178 bp, and an open reading frame (orf) of 1959 bp. the orf (kohsp70) was predicted to encode a polypeptide of 652 amino acids with a theoretical ...201525980488
molecular cloning and expression analysis of ghlof genes in upland cotton (gossypium hirsutum l.).shoot branching, i.e., the timing and position of shoot growth, determines to a large extend the pattern of plant architecture, and is the result of the integration of a plant's genetic background and environmental cues. many genes that are involved in the formation and outgrowth of axillary buds have been cloned, but the exact mechanism is still unclear. branching pattern is an important agronomic trait in many crops, including cotton. in the present study, we cloned four genes from cotton, and ...201525966231
development of gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the g. anomalum and g. hirsutum genomes.we reported the first development of gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. to continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as gossypium anomalum to cultivated allotetraploid cotton species. however, interspecific reproductive barriers limit gene transfer between species. in a previ ...201525957115
genetic mapping and comparative expression analysis of transcription factors in cotton.transcription factors (tfs) play an important role in the regulation of plant growth and development. the study of the structure and function of tfs represents a research frontier in plant molecular biology. the findings of these studies will provide significant information regarding genetic improvement traits in crops. currently, a large number of tfs have been cloned, and their function has been verified. however, relatively few studies that genetically map tfs in cotton are available. to gene ...201525946129
development of a 63k snp array for cotton and high-density mapping of intraspecific and interspecific populations of gossypium spp.high-throughput genotyping arrays provide a standardized resource for plant breeding communities that are useful for a breadth of applications including high-density genetic mapping, genome-wide association studies (gwas), genomic selection (gs), complex trait dissection, and studying patterns of genomic diversity among cultivars and wild accessions. we have developed the cottonsnp63k, an illumina infinium array containing assays for 45,104 putative intraspecific single nucleotide polymorphism ( ...201525908569
molecular mapping of restriction-site associated dna markers in allotetraploid upland cotton.upland cotton (gossypium hirsutum l., 2n = 52, aadd) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (snp) markers is difficult. the recent emergence of genome complexity reduction technologies based on the next-generation sequencing (ngs) platform has greatly expedited snp discovery in crops with highly repetitive and complex genomes. here we applied restriction-site associated dna (rad) sequencing technology for de novo snp discovery in allotetraploid cotton. we ...201525894395
sequencing of allotetraploid cotton (gossypium hirsutum l. acc. tm-1) provides a resource for fiber improvement.upland cotton is a model for polyploid crop domestication and transgenic improvement. here we sequenced the allotetraploid gossypium hirsutum l. acc. tm-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (bac)-end sequences and genotype-by-sequencing genetic maps. we assembled and annotated 32,032 a-subgenome genes and 34,402 d-subgenome genes. structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the a subgenome than i ...201525893781
genome sequence of cultivated upland cotton (gossypium hirsutum tm-1) provides insights into genome evolution.gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (atdt) genome. here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold bac-to-bac sequences and a high-resolution genetic map. in our assembly 88.5% of the 2,173-mb scaffolds, which cover 89.6%∼96.7% of the atdt genome, are anchored and oriented to 26 pseudochromosomes. comparison of this g. hirsutum atdt genome with the already sequenced diploid gossypium arboreum (aa) and gos ...201525893780
evaluating pilose, a cultigen of gossypium hirsutum, as a source of resistance to cotton fleahopper (hemiptera: miridae).cotton fleahopper (pseudatomoscelis seriatus reuter) (hemiptera: miridae) is a piercing-sucking insect that has emerged as a major pest of cotton (gossypium hirsutum l.) in texas. cotton fleahoppers feed on floral buds, commonly referred to as squares, causing damage and abscission, and subsequent yield loss. previous studies indicate that plant resistance to cotton fleahopper is present in upland cotton, but the mechanism of resistance remains undetermined. in this study, pilose, a cultigen of ...201526470352
in silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers.diversity of colors in flowers and fruits is largely due to anthocyanin pigments. the flavonoid/anthocyanin pathway has been most extensively studied. dihydroflavonol 4-reductase (dfr) is a vital enzyme of the flavonoid pathway which displays major impact on the formation of anthocyanins, flavan 3-ols and flavonols. the substrate specificity of the dfr was found to play a crucial role in determination of type of anthocyanidins. altering the flavonoid/anthocyanin pathway through genetic engineeri ...201526442064
photosynthetic characteristics of the subtending leaf of cotton boll at different fruiting branch nodes and their relationships with lint yield and fiber quality.to investigate photosynthetic characteristics of the subtending leaf at the 2-3rd and 10-11th fruiting branch (fbn, fb2-3, and fb10-11), and their relationship with cotton yield and quality, field experiments were conducted using two cotton cultivars, kemian 1 and sumian 15. the results showed that with fbn increasing, chlorophyll (chl) components, pn and non-photochemical quenching (npq) in the subtending leaf significantly declined, while soluble sugar, amino acid and their ratio (c ss/c aa) a ...201526442060
identification of early salt stress responsive proteins in seedling roots of upland cotton (gossypium hirsutum l.) employing itraq-based proteomic technique.soil salinity is a major abiotic stress that limits plant growth and agricultural productivity. upland cotton (gossypium hirsutum l.) is highly tolerant to salinity; however, large-scale proteomic data of cotton in response to salt stress are still scant. here, an isobaric tag for relative and absolute quantitation (itraq)-based proteomic technique was employed to identify the early differentially expressed proteins (deps) from salt-treated cotton roots. one hundred and twenty-eight deps were id ...201526442045
cadmium (cd) localization in tissues of cotton (gossypium hirsutum l.), and its phytoremediation potential for cd-contaminated soils.phytoremediation using economically valuable, large biomass, non-edible plants is a promising method for metal-contaminated soils. this study investigated cotton's tolerance for cd and remediation potential through analyzing cd bioaccumulation and localization in plant organs under different soil cd levels. results showed cotton presents good tolerance when soil cd concentration ≤20.26 mg kg(-1). cotton had good cd accumulation ability under low soil cd levels (<1.26 mg kg(-1)), with a tf value ...201526419249
herbicide-resistant cotton (gossypium hirsutum) plants: an alternative way of manual weed removal.cotton yield has been badly affected by different insects and weed competition. in past application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. the control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. to utilise the transgenic approach, cotton plants exp ...201526383095
overexpression of a cotton (gossypium hirsutum) wrky gene, ghwrky34, in arabidopsis enhances salt-tolerance of the transgenic plants.soil salinity is one of the most serious threats in world agriculture, and often influences cotton growth and development, resulting in a significant loss in cotton crop yield. wrky transcription factors are involved in plant response to high salinity stress, but little is known about the role of wrky transcription factors in cotton so far. in this study, a member (ghwrky34) of cotton wrky family was functionally characterized. this protein containing a wrky domain and a zinc-finger motif belong ...201526332661
comparisons of lygus lineolaris (hemiptera: miridae) populations from two distinct geographical regions of mississippi.the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), is a major pest of cotton (gossypium hirsutum l.) in the state of mississippi. economic data indicate that l. lineolaris is a more serious pest of cotton in the delta region of mississippi than in the hills region; however, little data exist comparing the two populations. two experiments were undertaken to compare l. lineolaris from these two geographically distinct regions. in the first experiment, colonies of ...201526313997
influence of crop management and environmental factors on wolf spider assemblages (araneae: lycosidae) in an australian cotton cropping system.wolf spiders (lycosidae) are the most abundant ground-hunting spiders in the australian cotton (gossypium hirsutum l.) agroecosystems. these spiders have potential in controlling pest bollworms, helicoverpa spp. (lepidoptera: noctuidae) in minimum-tilled fields. a study was carried out during a wet growing season (2011-2012) in narrabri, new south wales, australia, to determine how different crop rotations and tillage affect wolf spider assemblages in cotton fields. spider abundance and species ...201526308820
a high-density ssr genetic map constructed from a f2 population of gossypium hirsutum and gossypium darwinii.the cultivated allotetraploid species gossypium hirsutum, accounts for 90% of the world cotton production, has narrow genetic basis that's why its yield, quality or stress resistance breeding is stagnant. it is therefore, essential to explore desirable genes from gossypium darwinii which has enviable traits such as high fiber fineness, drought tolerance, fusarium and verticillium resistance. we used g. darwinii as primary plant materials in this study not only to enrich the genetic diversity of ...201526275937
high resolution consensus mapping of quantitative trait loci for fiber strength, length and micronaire on chromosome 25 of the upland cotton (gossypium hirsutum l.).cotton (gossypium hirsutum l.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. technological developments in the textile industry and improvements in human living standards have increased the requirement for supplies and better quality cotton. upland cotton 0-153 is an elite cultivar harboring strong fiber strength genes. to conduct quantitative trait locus (qtl) mapping for fiber quality in 0-153, we developed a population of 19 ...201526262992
transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress.salinity stress is one of the most devastating abiotic stresses in crop plants. as a moderately salt-tolerant crop, upland cotton (gossypium hirsutum l.) is a major cash crop in saline areas and a suitable model for salt stress tolerance research. in this study, we compared the transcriptome changes between the salt-tolerant upland cotton cultivar zhong 07 and salt-sensitive cultivar zhong g5 in response to nacl treatments. transcriptional regulation, signal transduction and secondary metabolism ...201526259172
construction of bac contig maps of homoeologous chromosomes a12 and d12 of gossypium hirsutum l. acc. tm-1.the gossypium hirsutum homoeologous chromosome 12 encodes important genes that contribute to fiber fuzz, lethality, gland development and male sterility. in this study a physical map of the cotton tm-1 chromosome 12 was constructed. a number of large-insert cotton genome libraries are available, and genome-wide physical mapping using large insert segments combined with bacterial cloning is a thriving area of genome research. however, sequencing of the cotton genome is difficult due to sequence r ...201526221184
the mitochondrial malate dehydrogenase 1 gene ghmmdh1 is involved in plant and root growth under phosphorus deficiency conditions in cotton.cotton, an important commercial crop, is cultivated for its natural fibers, and requires an adequate supply of soil nutrients, including phosphorus, for its growth. soil phosporus exists primarily in insoluble forms. we isolated a mitochondrial malate dehydrogenase (mdh) gene, designated as ghmmdh1, from gossypium hirsutum l. to assess its effect in enhancing p availability and absorption. an enzyme kinetic assay showed that the recombinant ghmmdh1 possesses the capacity to catalyze the intercon ...201526179843
a bil population derived from g. hirsutum and g. barbadense provides a resource for cotton genetics and breeding.to provide a resource for cotton genetics and breeding, an interspecific hybridization between gossypium hirsutum cv. emian22 and g. barbadense acc. 3-79 was made. a population of 54 bils (backcross inbred lines, bc1f8) was developed with the aim of transferring g. barbadense genes into g. hirsutum in order to genetically analyze these genes' function in a g. hirsutum background and create new germplasms for breeding. preliminary investigation of the morphological traits showed that the bils had ...201526517274
characterization and development of chloroplast microsatellite markers for gossypium hirsutum, and cross-species amplification in other gossypium species.cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. sixty chloroplast microsatellites were identified and characterized from the complete sequence of the gossypium hirsutum chloroplast genome using a bioinformatic approach. twenty chloroplast microsatellite loci were polymorphic in the 66 gossypium germplasm accessions. a total of 85 alleles were detected, with allele numbers varying from 2-7 per locus. po ...201526505340
leafy cotyledon1-casein kinase i-tcp15-phytochrome interacting factor4 network regulates somatic embryogenesis by regulating auxin homeostasis.somatic embryogenesis (se) is an efficient tool for the propagation of plant species and also, a useful model for studying the regulatory networks in embryo development. however, the regulatory networks underlying the transition from nonembryogenic callus to somatic embryos during se remain poorly understood. here, we describe an upland cotton (gossypium hirsutum) casein kinase i gene, ghcki, which is a unique key regulatory factor that strongly affects se. overexpressing ghcki halted the format ...201526491146
overexpression of ghwrky27a reduces tolerance to drought stress and resistance to rhizoctonia solani infection in transgenic nicotiana benthamiana.wrky proteins constitute transcriptional regulators involved in various biological processes, especially in coping with diverse biotic and abiotic stresses. however, in contrast to other well-characterized wrky groups, the functions of group iii wrky transcription factors are poorly understood in the economically important crop cotton (gossypium hirsutum). in this study, a group iii wrky gene from cotton, ghwrky27a, was isolated and characterized. our data indicated that ghwrky27a localized to t ...201526483697
dynamic transcriptome analysis and volatile profiling of gossypium hirsutum in response to the cotton bollworm helicoverpa armigera.in response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. however, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (cbw; helicoverpa armigera) larv ...201526148847
promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton flowering locus t (ft)-like gene ghft1.flowering locus t (ft) encodes a mobile signal protein, recognized as major component of florigen, which has a central position in regulating flowering, and also plays important roles in various physiological aspects. a mode is recently emerging for the balance of indeterminate and determinate growth, which is controlled by the ratio of ft-like and terminal flower 1 (tfl1)-like gene activities, and has a strong influence on the floral transition and plant architecture. orthologs of ghft1 was pre ...201526136765
predawn respiration rates during flowering are highly predictive of yield response in gossypium hirsutum when yield variability is water-induced.respiratory carbon evolution by leaves under abiotic stress is implicated as a major limitation to crop productivity; however, respiration rates of fully expanded leaves are positively associated with plant growth rates. given the substantial sensitivity of plant growth to drought, it was hypothesized that predawn respiration rates (rpd) would be (1) more sensitive to drought than photosynthetic processes and (2) highly predictive of water-induced yield variability in gossypium hirsutum. two stu ...201526125121
bac-end sequence-based snp mining in allotetraploid cotton (gossypium) utilizing resequencing data, phylogenetic inferences, and perspectives for genetic mapping.a bacterial artificial chromosome library and bac-end sequences for cultivated cotton (gossypium hirsutum l.) have recently been developed. this report presents genome-wide single nucleotide polymorphism (snp) mining utilizing resequencing data with bac-end sequences as a reference by alignment of 12 g. hirsutum l. lines, one g. barbadense l. line, and one g. longicalyx hutch and lee line. a total of 132,262 intraspecific snps have been developed for g. hirsutum, whereas 223,138 and 470,631 inte ...201525858960
cottonseed protein, oil, and mineral status in near-isogenic gossypium hirsutum cotton lines expressing fuzzy/linted and fuzzless/linted seed phenotypes under field conditions.cotton is an important crop in the world and is a major source of oil for human consumption and cotton meal for livestock. cottonseed nutrition (seed composition: protein, oil, and minerals) determines the quality of seeds. therefore, maintaining optimum levels of cottonseed nutrition is critical. physiological and genetic mechanisms controlling the levels of these constituents in cottonseed are still largely unknown. our previous research conducted under greenhouse conditions showed that seed a ...201525852704
genome-wide analysis of the family 1 glycosyltransferases in cotton.family 1 gt, designated as ugt, is the largest and most functionally important multigene family in the plant kingdom. in this study, we carried out a genome-wide identification, analysis, and comparison of 142, 146, and 196 putative ugts from gossypium raimondii, gossypium arboreum, and gossypium hirsutum, respectively. all members present the 44 amino-acid conserved consensus sequence termed the plant secondary product glycosyltransferase motif. according to the phylogenetic relationship among ...201525851236
global analysis of the gossypium hirsutum l. transcriptome during leaf senescence by rna-seq.leaf senescence is an important developmental programmed degeneration process that dramatically affects crop quality and yield. the regulation of senescence is highly complex. although senescence regulatory genes have been well characterized in model species such as arabidopsis and rice, there is little information on the control of this process in cotton. here, the senescence process in cotton (gossypium hirsutum l.) leaves was investigated over a time course including young leaf, mature leaf a ...201525849479
rna-seq analysis of short fiber mutants ligon-lintless-1 (li 1 ) and - 2 (li 2 ) revealed important role of aquaporins in cotton (gossypium hirsutum l.) fiber elongation.cotton fiber length is a key determinant of fiber quality for the textile industry. understanding the molecular basis of fiber elongation would provide a means for improvement of fiber length. ligon lintless-1 (li 1 ) and ligon lintless-2 (li 2 ) are monogenic and dominant mutations, that result in an extreme reduction in the length of lint fiber to approximately 6 mm on mature seeds. in a near-isogenic state with wild type (wt) cotton these two short fiber mutants provide an excellent model sys ...201525848981
genetic diversity, population structure and marker trait associations for seed quality traits in cotton (gossypium hirsutum).cottonseed contains 16% seed oil and 23% seed protein by weight. high levels of palmitic acid provides a degree of stability to the oil, while the presence of bound gossypol in proteins considerably changes their properties, including their biological value. this study uses genetic principles to identify genomic regions associated with seed oil, protein and fibre content in upland cotton cultivars. cotton association mapping panel representing the us germplasm were genotyped using amplified frag ...201525846880
down-regulation of the cotton endo-1,4-β-glucanase gene kor1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour.towards the aim of examining the potential function of korrigan (kor), a highly conserved membrane-bound endoglucanase, in reproductive development, here transgenic evidence is provided that a cotton (gossypium hirsutum) endoglucanase, ghkor1, plays significant roles in endosperm and embryo development. rna interference (rnai)- and co-suppression-mediated down-regulation of ghkor1 resulted in smaller filial tissue and reduced seed weight, which were characterized by disrupted endosperm cellulari ...201525805716
distribution and evolution of cotton fiber development genes in the fibreless gossypium raimondii genome.cotton fiber represents the largest single cell in plants and they serve as models to study cell development. this study investigated the distribution and evolution of fiber unigenes anchored to recombination hotspots between tetraploid cotton (gossypium hirsutum) at and dt subgenomes, and within a parental diploid cotton (gossypium raimondii) d genome. comparative analysis of at vs d and dt vs d showed that 1) the d genome provides many fiber genes after its merger with another parental diploid ...201525796538
construction of a high-density genetic map and lint percentage and cottonseed nutrient trait qtl identification in upland cotton (gossypium hirsutum l.).upland cotton plays a critical role not only in the textile industry, but also in the production of important secondary metabolites, such as oil and proteins. construction of a high-density linkage map and identifying yield and seed trait quantitative trail loci (qtl) are prerequisites for molecular marker-assisted selective breeding projects. here, we update a high-density upland cotton genetic map from recombinant inbred lines. a total of 25,313 ssr primer pairs were screened for polymorphism ...201525796191
rnai construct of a cytochrome p450 gene cyp82d109 blocks an early step in the biosynthesis of hemigossypolone and gossypol in transgenic cotton plants.naturally occurring terpenoid aldehydes from cotton, such as hemigossypol, gossypol, hemigossypolone, and the heliocides, are important components of disease and herbivory resistance in cotton. these terpenoids are predominantly found in the glands. differential screening identified a cytochrome p450 cdna clone (cyp82d109) from a gossypium hirsutum cultivar that hybridized to mrna from glanded cotton but not glandless cotton. both the d genome cotton gossypium raimondii and a genome cotton gossy ...201525794893
complete genome sequences of two new virus isolates associated with cotton blue disease resistance breaking in brazil.since 2006, brazilian cotton (gossypium hirsutum) crops planted with cultivars that are resistant to cotton blue disease have developed a new disease termed "atypical" cotton blue disease or atypical vein mosaic disease. here, we describe the complete genomes of two virus isolates associated with this disease. the new virus isolates, called clrdv-acr3 and clrdv-ima2, were found to have a high degree of nucleotide and amino acid sequence similarity to previously described isolates of cotton leafr ...201525772571
cotton qtldb: a cotton qtl database for qtl analysis, visualization, and comparison between gossypium hirsutum and g. hirsutum × g. barbadense populations.a specialized database currently containing more than 2200 qtl is established, which allows graphic presentation, visualization and submission of qtl. in cotton quantitative trait loci (qtl), studies are focused on intraspecific gossypium hirsutum and interspecific g. hirsutum × g. barbadense populations. these two populations are commercially important for the textile industry and are evaluated for fiber quality, yield, seed quality, resistance, physiological, and morphological trait qtl. with ...201525758743
construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (gossypium hirsutum l.).the first high-density linkage map was constructed to identify quantitative trait loci (qtls) for somatic embryogenesis (se) in cotton ( gossypium hirsutum l.) using leaf petioles as explants. cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (se) in cotton (gossypium hirsutum l.). to construct a more saturated linkage map and further identify quantitative trait loci (qtls) for se ...201525758337
gene-expression novelty in allopolyploid cotton: a proteomic perspective.allopolyploidization is accompanied by changes in gene expression that are thought to contribute to phenotypic diversification. here we describe global changes in the single-celled cotton fiber proteome of two natural allopolyploid species (gossypium hirsutum and g. barbadense) and living models of their diploid parents using two different proteomic approaches. in total, 1323 two-dimensional gel electrophoresis spots and 1652 identified proteins by isobaric tags for relative and absolute quantit ...201525735302
targeted lipidomics studies reveal that linolenic acid promotes cotton fiber elongation by activating phosphatidylinositol and phosphatidylinositol monophosphate biosynthesis.the membrane lipids from fast-elongating wild-type cotton (gossypium hirsutum) fibers at 10 days post-anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. fiber cells contained significantly higher amounts of phosphatidylinositol (pi) than both ovule samples with pi 34:3 being the most predominant species. the genes encoding fatty acid desaturases (δ(15)ghfad), pi synthase (pis) and ...201525731673
overexpression of cotton rav1 gene in arabidopsis confers transgenic plants high salinity and drought sensitivity.rav (related to abi3/vp1) protein containing an ap2 domain in the n-terminal region and a b3 domain in the c-terminal region, which belongs to ap2 transcription factor family, is unique in higher plants. in this study, a gene (ghrav1) encoding a rav protein of 357 amino acids was identified in cotton (gossypium hirsutum). transient expression analysis of the egfp:ghrav1 fusion genes in tobacco (nicotiana tabacum) epidermal cells revealed that ghrav1 protein was localized in the cell nucleus. qua ...201525710493
comparative proteomic and biochemical analyses reveal different molecular events occurring in the process of fiber initiation between wild-type allotetraploid cotton and its fuzzless-lintless mutant.to explore lint fiber initiation-related proteins in allotetraploid cotton (gossypium hirsutum l.), a comparative proteomic analysis was performed between wild-type cotton (xu-142) and its fuzzless-lintless mutant (xu-142-fl) at five developmental time points for lint fiber initiation from -3 to +3 days post-anthesis (dpa). using two-dimensional gel electrophoresis (2-de) combined with mass spectrometry (ms) analyses, 91 differentially accumulated protein (dap) species that are related to fiber ...201525700002
anther response to high-temperature stress during development and pollen thermotolerance heterosis as revealed by pollen tube growth and in vitro pollen vigor analysis in upland cotton.pollen tube growth in styles was strongly inhibited by temperature above 35 °c, and the yield of cotton decreased because of the adverse effect of high temperatures during square development. high-temperature stress during flowering influences the square development of upland cotton (gossypium hirsutum l.) and cotton yield. although it is well known that square development is sensitive to high temperature, high-temperature sensitive stages of square development and the effects of high temperatur ...201525672505
enrichment of an intraspecific genetic map of upland cotton by developing markers using parental rad sequencing.rad sequencing was performed using dh962 and jimian5 as upland cotton mapping parents. sequencing data for dh962 and jimian5 were assembled into the genome sequences of ≈55.27 and ≈57.06 mb, respectively. analysing genome sequences of the two parents, 1,323 ssr, 3,838 insertion/deletion (indel), and 9,366 single-nucleotide polymorphism (snp) primer pairs were developed. all of the ssrs, 121 indels, 441 snps, and other 6,747 primer pairs were screened in the two parents, and a total of 535 new po ...201525656006
measurements of transpiration isotopologues and leaf water to assess enrichment models in cotton.the two-pool and péclet effect models represent two theories describing mechanistic controls underlying leaf water oxygen isotope composition at the whole-leaf level (δ(18) ol ). to test these models, we used a laser spectrometer coupled to a gas-exchange cuvette to make online measurements of δ(18) o of transpiration (δ(18) otrans ) and transpiration rate (e) in 61 cotton (gossypium hirsutum) leaves. δ(18) otrans measurements permitted direct calculation of δ(18) o at the sites of evaporation ( ...201525643590
lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton.the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations.201524777962
dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (gossypium hirsutum).the soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (rflp), terminal restriction length polymorphism (t-rflp), and cloning-sequencing. the cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. phylogenetic and diversity analysis of sequences obtained from the f ...201524677076
tillage, cropping systems, and nitrogen fertilizer source effects on soil carbon sequestration and fractions.quantification of soil carbon (c) cycling as influenced by management practices is needed for c sequestration and soil quality improvement. we evaluated the 10-yr effects of tillage, cropping system, and n source on crop residue and soil c fractions at 0- to 20-cm depth in decatur silt loam (clayey, kaolinitic, thermic, typic paleudults) in northern alabama, usa. treatments were incomplete factorial combinations of three tillage practices (no-till [nt], mulch till [mt], and conventional till [ct ...201518453410
microrna evolution and expression analysis in polyploidized cotton genome.cotton (gossypium hirsutum l.), the most important fibre plant in the world, is a tetraploid species, originating from the reunion of two ancestral cotton species ~1-2 million years ago. it has been reported that a great number of genes were quickly erased or preferentially remained after whole-genome duplication, ultimately leading to morphogenesis evolution. however, micrornas (mirnas), a new class of gene regulators, have not been well studied in polyploidization. here, we systematically inve ...201525561162
physiological performance and differential expression profiling of genes associated with drought tolerance in contrasting varieties of two gossypium species.cotton is mostly cultivated under rain-fed conditions in india, thus faces frequent drought conditions during its life cycle. drought being a major stress factor responsible for yield penalty, there has always been a high priority to generate knowledge on adaptation and tolerance of cotton. in the present study, four cotton varieties, jkc-770 and kc-2 (gossypium hirsutum), and jkc-717 and rahs-187(gossypium herbaceum), were imposed to drought. under drought condition, differential changes in phy ...201525149149
the cotton wrky gene ghwrky41 positively regulates salt and drought stress tolerance in transgenic nicotiana benthamiana.wrky transcription factors constitute a very large family of proteins in plants and participate in modulating plant biological processes, such as growth, development and stress responses. however, the exact roles of wrky proteins are unclear, particularly in non-model plants. in this study, gossypium hirsutum wrky41 (ghwrky41) was isolated and transformed into nicotiana benthamiana. our results showed that overexpression of ghwrky41 enhanced the drought and salt stress tolerance of transgenic ni ...201526562293
analysis of upland cotton (gossypium hirsutum) response to verticillium dahliae inoculation by transcriptome sequencing.verticillium wilt is one of the main diseases in cotton (gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. at present, the molecular mechanism that confers resistance to this disease is unclear. transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. in this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with verticillium da ...201526535625
microrna and mrna expression profiling analysis revealed the regulation of plant height in gossypium hirsutum.dwarf cottons are more resistant to damage from wind and rain and associated with stable, increased yields, and also desirable source for breeding the machine harvest varieties. in an effort to uncover the transcripts and mirna networks involved in plant height, the transcriptome and small rna sequencing were performed based on dwarf mutant ari1327 (a1), tall-culm mutant ari3697 (a3) and wild type ari971 (a9) in gossypium hirsutum.201526517985
partial dominance, overdominance, epistasis and qtl by environment interactions contribute to heterosis in two upland cotton hybrids.based on two recombinant inbred line (ril) populations, two corresponding backcross (bc) populations were constructed to elucidate the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). the yield, and yield components, of these populations were evaluated in three environments. at the single-locus level, 78 and 66 quantitative trait loci (qtl) were detected using composite interval mapping in ril and bc populations, respectively, and 29 qtl were identified based on mid-parental ...201526715091
atrav1 and atrav2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering.there is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. to better understand drought stress signaling and adaptation in cotton (gossypium hirsutum) fiber development, we expressed the arabidopsis transcription factors related_to_aba-insensitive3/viviparous1/(rav1) and atrav2, which encode apetala2-basic3 domain proteins shown to repress transcription of flowering_locus_t (ft) and to promote stomatal opening cell-autonomously. in three years o ...201526706061
polyamine and its metabolite h2o2 play a key role in the conversion of embryogenic callus into somatic embryos in upland cotton (gossypium hirsutum l.).the objective of this study was to increase understanding about the mechanism by which polyamines (pas) promote the conversion of embryogenic calli (ec) into somatic embryos in cotton (gossypium hirsutum l.). we measured the levels of endogenous pas and h2o2, quantified the expression levels of genes involved in the pas pathway at various stages of cotton somatic embryogenesis (se), and investigated the effects of exogenous pas and h2o2 on differentiation and development of ec. putrescine (put), ...201526697030
delineating the glycoproteome of elongating cotton fiber cells.the data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and n-linked glycosylation site identification (kumar et al., 2013) [1]. utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. in this context we have reanalyzed our glycoproteome dataset with the gossypium arbor ...201526693171
microarray-based large scale detection of single feature polymorphism in gossypium hirsutum l.microarrays offer an opportunity to explore the functional sequence polymorphism among different cultivars of many crop plants. the affymetrix microarray expression data of five genotypes of gossypium hirsutum l. at six different fibre developmental stages was used to identify single feature polymorphisms (sfps). the background corrected and quantile-normalized log2 intensity values of all probes of triplicate data of each cotton variety were subjected to sfps call by using sam procedure in r la ...201526690522
partial dominance, overdominance and epistasis as the genetic basis of heterosis in upland cotton (gossypium hirsutum l.).determination of genetic basis of heterosis may promote hybrid production in upland cotton (gossypium hirsutum l.). this study was designed to explore the genetic mechanism of heterosis for yield and yield components in f2: 3 and f2: 4 populations derived from a hybrid 'xinza no. 1'. replicated yield field trials of the progenies were conducted in 2008 and 2009. phenotypic data analyses indicated overdominance in f1 for yield and yield components. additive and dominance effects at single-locus l ...201526618635
data for proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.).cotton is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to study the male sterile mechanisms of ccri9106, histological and itraq-facilitated proteomic analyses of anthers were performed. this data article contains data related to the research article titled itraq-facilitated proteomic profiling of ...201526958592
stable transformation and expression of ghexpa8 fiber expansin gene to improve fiber length and micronaire value in cotton.cotton fiber is multigenic trait controlled by number of genes. previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. in the present study, the gossypium hirsutum ghexpa8 fiber expansin gene was introduced into local cotton variety niab 846 by using an agrobacterium-mediated gene transformation. the neomycin phosphotransferase (nptii) gene was used as a selection marker for scre ...201526583018
a and d genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant.chromosomal dispositions were analyzed on the metaphase plate of tetraploid cotton (aadd). at metaphase, the two subgenomes, a and d, were separated in a radial pattern in which the small d subgenome chromosomes tended to concentrate at the center and the large a subgenome chromosomes were scattered about the periphery on the metaphase plate. although the ordered chromosome arrangement was disturbed in an artificial hexaploid (aaddgg), the separation pattern could be recovered after the majority ...201526568399
constructing a high-density linkage map for gossypium hirsutum × gossypium barbadense and identifying qtls for lint percentage.to introgress the good fiber quality and yield from gossypium barbadense into a commercial upland cotton variety, a high-density simple sequence repeat (ssr) genetic linkage map was developed from a bc1 f1 population of gossypium hirsutum × gossypium barbadense. the map comprised 2,292 loci and covered 5115.16 centimorgan (cm) of the cotton ad genome, with an average marker interval of 2.23 cm. of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on ...201525263268
variovorax gossypii sp. nov., isolated from gossypium hirsutum.a beige-pigmented bacterial strain (jm-310t), isolated from the healthy internal root tissue of 4-week-old cotton (gossypium hirsutum, cultivar 'des-119') in tallassee (macon county), alabama, usa, was studied taxonomically. the isolate produced small rod-shaped cells, which showed a gram-negative staining behaviour. a comparison of the 16s rrna gene sequence of the isolate revealed 99.2, 98.8, 98.7, 98.7, 98.1 and 97.6 % similarity to the 16s rrna gene sequences of the type strains of variovora ...201526341669
a novel nap member ghnap is involved in leaf senescence in gossypium hirsutum.premature leaf senescence has a negative influence on the yield and quality of cotton, and several genes have been found to regulate leaf senescence. howeer, many underlying transcription factors are yet to be identified. in this study, a nap-like transcription factor (ghnap) was isolated from gossypium hirsutum. ghnap has the typical nac structure and a conserved novel subdomain in its divergent transcription activation region (tar). ghnap was demonstrated to be a nuclear protein, and it showed ...201525991739
analyzing serial cdna libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of upland cotton (gossypium hirsutum l.).by comparing series full-length cdna libraries stressed and control, the dynamic process of salt stress response in upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. the upland cotton is the most important fiber plant with highly salt tolerance. however, the molecular mechanism underlying salt tolerance in domesticated cotton was unclear. here, seven full-length cdna libraries were constructed for seedling roots of upland cotton 'zhong g 5' ...201525700980
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. hirsutum.in this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation.an itraq-based proteomics of ovules from the upland cotton species gossypium hirsutum and its fuzzless-lintless mutant was performed, and finally 2729 proteins that preferentially accumulated at anthesis in wild-type ovules were identified. we confirmed that the gene expression levels of 2005 among these proteins also increased by performing an rna sequencing transcriptomics. expression of proteins involved in carboxylic acid metabolism, small-molecule metabolic processes, hormone regulation, an ...201525449837
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
transcriptome analysis of cotton (gossypium hirsutum l.) genotypes that are susceptible, resistant, and hypersensitive to reniform nematode (rotylenchulus reniformis).reniform nematode is a semi-endoparasitic nematode species causing significant yield loss in numerous crops, including cotton (gossypium hirsutum l.). an rna-sequencing analysis was conducted to measure transcript abundance in reniform nematode susceptible (dp90 & sg747), resistant (barbren-713), and hypersensitive (lonren-1) genotypes of cotton (gossypium hirsutum l.) with and without reniform nematode infestation. over 90 million trimmed high quality reads were assembled into 84,711 and 80, 35 ...201526571375
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems.aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ...201526241862
molecular cloning and expression analysis of five ghraxs in upland cotton (gossypium hirsutum l.).the formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. several transcription factor-encoding genes, including cuc3, rax, las, lof1, and rox, have been cloned by screening for axillary meristem mutants in arabidopsis thaliana. these genes will facilitate our understanding of the mechanisms underlying axillary meristem development. in this study, we report the cloning of five genes from cotton (goss ...201526505359
kinesin-4 functions in vesicular transport on cortical microtubules and regulates cell wall mechanics during cell elongation in plants.in plants, anisotropic cell expansion depends on cortical microtubules that serve as tracks along which macromolecules and vesicles are transported by the motor kinesins of unknown identities. we used cotton (gossypium hirsutum) fibers that underwent robust elongation to discover kinesins that are involved in cell elongation and found gh kinesin-4a expressed abundantly. the motor was detected by immunofluorescence on vesicle-like structures that were associated with cortical microtubules. in ara ...201525600279
temperature responses of mesophyll conductance differ greatly between species.the temperature responses of mesophyll conductance (gm ) were investigated for nine species using carbon isotope techniques combining tunable diode laser spectroscopy and gas exchange measurements. species included the evergreen trees eucalyptus pauciflora and quercus engelmannii; the tropical evergreen tree lophostemon confertus; as well as the herbaceous species nicotiana tabacum, oryza sativa, triticum aestivum, gossypium hirsutum, glycine max and arabidopsis thaliana. responses varied from a ...201525224884
interference between redroot pigweed (amaranthus retroflexus l.) and cotton (gossypium hirsutum l.): growth analysis.redroot pigweed is one of the injurious agricultural weeds on a worldwide basis. understanding of its interference impact in crop field will provide useful information for weed control programs. the effects of redroot pigweed on cotton at densities of 0, 0.125, 0.25, 0.5, 1, 2, 4, and 8 plants m(-1) of row were evaluated in field experiments conducted in 2013 and 2014 at institute of cotton research, caas in china. redroot pigweed remained taller and thicker than cotton and heavily shaded cotton ...201526057386
host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore.insect herbivores may undergo genetic divergence on their host plants through host-associated differentiation (had). much of what we know about had involves insect species with narrow host ranges (i.e., specialists) that spend part or all their life cycle inside their hosts, and/or reproduce asexually (e.g., parthenogenetic insects), all of which are thought to facilitate had. however, sexually reproducing polyphagous insects can also exhibit had. few sexually reproducing insects have been teste ...201526257868
relationship between piercing-sucking insect control and internal lint and seed rot in southeastern cotton (gossypium hirsutum l.).in 1999, crop consultants scouting for stink bugs (hemiptera spp.) in south carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% in certain fields. the disease has subsequently been reported in fields throughout the southeastern cotton belt. externally, diseased bolls appeared undamaged; internally, green fruit contain pink to dark brown, damp, deformed lint, and necrotic seeds. in greenhouse experiments, we demonstrated transmission of the ...201526470293
overexpression of mic-3 indicates a direct role for the mic gene family in mediating upland cotton (gossypium hirsutum) resistance to root-knot nematode (meloidogyne incognita).transgene-based analysis of the mic-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. major quantitative trait loci have been mapped to upland cotton (gossypium hirsutum l.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (rkn; meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this rkn-resistant phenot ...201525376794
functional characterization of an anthocyanidin reductase gene from the fibers of upland cotton (gossypium hirsutum).metabolic profiling, gene cloning, enzymatic analysis, ectopic expression, and gene silencing experiments demonstrate that the anthocyanidin reductase (anr) pathway is involved in the biosynthesis of proanthocyanidins in upland cotton. proanthocyanidins (pas) are oligomeric or polymeric flavan-3-ols, however, the biosynthetic pathway of pas in cotton remains to be elucidated. here, we report on an anthocyanidin reductase (anr) gene from cotton fibers and the anr pathway of pas. phytochemical ana ...201525575669
sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens.genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ...201526483808
analyses of fusarium wilt race 3 resistance in upland cotton (gossypium hirsutum l.).fusarium wilt [fusarium oxysporum f.sp. vasinfectum (fov) atk. sny & hans] represents a serious threat to cotton (gossypium spp.) production. for the last few decades, the fov pathogen has become a significant problem in uzbekistan causing severe wilt disease and yield losses of g. hirsutum l. cultivars. we present the first genetic analyses of fov race 3 resistance on uzbek cotton germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. the fie ...201525896369
tillage impact on herbicide loss by surface runoff and lateral subsurface flow.there is worldwide interest in conservation tillage practices because they can reduce surface runoff, and agrichemical and sediment losses from farm fields. since these practices typically increase infiltration, their use may increase subsurface transport of water-soluble contaminants. thus, to assess long-term environmental benefits of conservation tillage data may be needed that quantify both surface and subsurface contaminant fluxes. this study focused on the herbicide fluometuron (n,n-dimeth ...201526057540
spatial and temporal dynamics of stink bugs in southeastern farmscapes.a 3-yr study (2009-2011) was conducted to examine the spatial and temporal dynamics of stink bugs in three commercial farmscapes. study locations were replicated in south carolina and georgia, in an agriculturally diverse region known as the southeastern coastal plain. crops included wheat, triticum aestivum (l.), corn, zea mays (l.), soybean, glycine max (l.), cotton, gossypium hirsutum (l.), and peanut, arachis hypogaea (l.). farmscapes were sampled weekly using whole-plant examinations for co ...201525843577
determining the major bt refuge crops for cotton bollworm in north china.evaluation of the effectiveness of refuge strategies involved in cotton bollworm bt resistance management would be aided by technologies that allow monitoring and quantification of key factors that affect the process under field conditions. we hypothesized that characterization of stable carbon and nitrogen isotopes in adult bollworm, helicoverpa armigera (hübner) (lepidoptera: noctuidae) moths may aid in determining the larval host that they developed upon. we found moths reared from larvae fed ...201525196072
molecular cloning of a dehydration-responsive protein gene (mrd22) from mulberry, and determination of abiotic stress patterns of mrd22 gene expression.a full-length cdna sequence coding for dehydration-responsive protein gene of mulberry tree, which we designated was mrd22 (genbank accession number: jq804833) was cloned based on mulberry expressed sequence tags (ests). mrd22 is 1503 bp long, contains a 334 bp 5'-utr (untranslated region) and a 563 bp 3'-utr, encodes 201 amino acids with a predicted molecular weight of 54.28 kda and an isoelectric point of 9.35. phylogenetic analysis based on mrd22 sequences from different species showed that m ...201525895340
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