Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| prevalence of genital mycoplasmas in asymptomatic male partners of women diagnosed as having chlamydial infections. | we examined 209 asymptomatic male partners of women diagnosed as having chlamydial infections for the prevalence of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum in their first-voided urine (fvu) by nucleic acid amplification tests. quantification of leukocytes in fvu was performed by automated urine particle analyzers. two (1.0%) men were positive for n. gonorrhoeae, and 92 (44.0%) were positive for c. trac ... | 2014 | 24486047 |
| prevalence of sexually transmitted infections among healthy korean women: implications of multiplex pcr pathogen detection on antibiotic therapy. | in this study, we investigated the prevalence of sexually transmitted infections (stis) using multiplex real-time pcr assay in healthy korean women. we also evaluated the risk factors of stis, and compared the various factors between the sti-positive and the sti-negative groups. a total of 799 endocervical swab samples from healthy korean women who visited our hospital for general medical check-ups during january 2012 to october 2012 were included. eight stis including human papillomavirus (hpv) ... | 2014 | 24462432 |
| prevalence of ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis infections, and semen quality in infertile and fertile men in china. | to study the prevalence of ureaplasma urealyticum (uu), mycoplasma hominis (mh), and chlamydia trachomatis (ct) infections among infertile and fertile men and to study the effects of these infections on semen quality. | 2014 | 24411218 |
| [antimycoplasmic activity of fermentation broth of trichoderma harzianum rifai f-180, an organism producing l-lysine-α-oxidase, an antitumor and antiviral enzyme]. | a concentrate of the fermentation broth of trichoderma harzianum rifai f-180, an organism producing l-lysine-α-oxidase, an antitumor and antiviral enzyme, with the activity in the fermentation broth of 0.54-0.56 u/mi was recovered. the effect of the concentrate on the mycoplasmas growth was investigated for the first time. two representatives of mycoplasmafaceae, i.e. mycoplasma hominis and mycoplasma fermentans and one representative of aholeplasmataceae. i. e. aholeplasma laidlawii were used. ... | 2014 | 26448986 |
| detection of genital mycoplasmal infections among infertile females by multiplex pcr. | women reproductive system is a suitable environment for growth of various pathogen and nonpathogenic microorganisms. mycoplasmataceae is a family of bacteria which cause oligosymptomatic genital infections. the complications caused by these bacteria may lead to infertility in women. the aim of this study was detection of genital mycoplasma hominis, ureaplasmaurealyticum and mycoplasma genitalium among infertile females who referred to the infertility clinics. | 2014 | 25926957 |
| isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. | mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. | 2014 | 25469132 |
| ureaplasmas and mycoplasmas in vaginal samples from prepubertal girls and the reasons for gynecological consultation. | the aim of the study was to evaluate vaginal colonization with ureaplasmaurealyticum (uu) and mycoplasma hominis (mh) in prepubertal girls and reason for gynecological consultation. | 2014 | 24268557 |
| microbial load of umbilical cord blood ureaplasma species and mycoplasma hominis in preterm prelabor rupture of membranes. | to evaluate ureaplasma species and m. hominis dna in the umbilical cord blood and its correlation with its microbial load in the amniotic fluid, as a measure of microbial burden in fetal inflammatory response and neonatal outcome in pregnancies complicated by preterm prelabor rupture of membranes (pprom). | 2014 | 24450299 |
| antimicrobial susceptibility of ureaplasma urealyticum and mycoplasma hominis. | 2014 | 24366778 | |
| a case report of mycoplasma hominis brain abscess identified by maldi-tof mass spectrometry. | we report the case of a 43-year-old man with a mycoplasma hominis brain abscess occurring after a cranial trauma, which was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the presence of colonies on classic blood agar plates and the use of maldi-tof ms, a valuable diagnostic tool that identified m. hominis due to its presence in the vitek ms database, allowed the rapid diagnosis of this infection. | 2014 | 25449252 |
| performance of four commercial kits for laboratory diagnosis of urogenital mollicute infection. | we have empirical evidence for the unsatisfactory performance of the most accessible commercial kits for mollicute culture. we aimed to evaluate the performance of the 4 commercial kits available for diagnosis of urogenital mollicute infections, compared with culture media (cmmc) produced in our research laboratory. we demonstrated that the newprov kit had a sensitivity of 17% for ureaplasma sp. and 33% for mycoplasma hominis. the laborclin kit presented a sensitivity of 25% for ureaplasma sp., ... | 2014 | 25188579 |
| [measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods]. | establishment of ratios that would allow to execute recalculation of mycoplasma concentration from cfu/ml and/or ccu/ml into units obtained during pcr analysis--geq/ml. | 2014 | 25051693 |
| epidemiological investigation and antimicrobial susceptibility analysis of ureaplasma species and mycoplasma hominis in outpatients with genital manifestations. | the aim of this study was to assess the prevalence and drug resistance of ureaplasma species and mycoplasma hominis in outpatients with genital manifestation from 2005 to 2013 in hangzhou, china. | 2014 | 24982440 |
| molecular mechanism of fluoroquinolones resistance in mycoplasma hominis clinical isolates. | to evaluate the molecular mechanism of fluoroquinolones resistance in mycoplasma hominis (mh) clinical strains isolated from urogenital specimens. 15 mh clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (qrdrs) of dna gyrase (gyra and gyrb) and topoisomerase iv (parc and pare) in comparison with the reference strain pg21, which is susceptible to fluoroquinolones antibiotics. 15 mh ... | 2014 | 24948939 |
| a potential pathogenic factor from mycoplasma hominis is a tlr2-dependent, macrophage-activating, p50-related adhesin. | mycoplasma hominis has been implicated in many inflammatory conditions of the human urogenital tract in particular amniotic infections that lead to fetal and neonatal disease and pre-term labor. the mechanisms responsible are poorly defined. | 2014 | 24938999 |
| intra-abdominal mycoplasma hominis infection in a liver transplant recipient: a case report. | 2014 | 24858617 | |
| postoperative mycoplasma hominis infections after neurosurgical intervention. | mycoplasma hominis is a rare cause of infection after neurosurgical procedures. the mycoplasma genus contains the smallest bacteria discovered to date. mycoplasma are atypical bacteria that lack a cell wall, a feature that complicates both diagnosis and treatment. the gram stain and some types of culture media fail to identify these organisms, and typical broad-spectrum antibiotic regimens are ineffective because they act on cell wall metabolism. mycoplasma hominis commonly colonizes the genitou ... | 2014 | 24856879 |
| treatment of genital mycoplasma in colonized pregnant women in late pregnancy is associated with a lower rate of premature labour and neonatal complications. | mycoplasma hominis and ureaplasma spp. may colonize the human genital tract and have been associated with adverse pregnancy outcomes such as preterm labour and preterm premature rupture of membranes. however, as these bacteria can reside in the normal vaginal flora, there are controversies regarding their true role during pregnancy and so the need to treat these organisms. we therefore conducted a retrospective analysis to evaluate the treatment of genital mycoplasma in 5377 pregnant patients sh ... | 2014 | 24849820 |
| colonization of mycoplasma hominis and ureaplasma urealyticum in pregnant women and their transmission to offspring. | mycoplasma hominis and ureaplasma urealyticum are important opportunistic pathogens that cause urogenital infections and accelerated newborn delivery in pregnant women. moreover genital mycoplasmas have been implicated in different neonatal diseases such as pneumonia, sepsis and meningitis. this study was conducted to find out the prevalence and transmission rate of these two organisms in pregnant women and their neonates. | 2014 | 25802703 |
| isolation and molecular identification of mycoplasma hominis in infertile female and male reproductive system. | infection of urogenital system with mycoplasma potentially affect reproductive system and increases infants mortalities. therefore, detection of these organisms is an important issue that should be considered and appropriate diagnostic methods should be used to identify these microorganisms. in the female reproductive system, infection can affect different parts of the cervix, endometrium, and fallopian tube. the extent of this infection in different diseases and its pathogenesis might be relate ... | 2014 | 25738116 |
| genome-wide analysis of mycoplasma hominis for the identification of putative therapeutic targets. | ever increasing propensity of antibiotic resistance among pathogenic bacteria raises the demand for the development of novel therapeutic agents to control this grave problem. advances in the field of bioinformatics, genomics, and proteomics have greatly facilitated the discovery of alternative drugs by swift identification of new drug targets. in the present study, we employed comparative genomics and metabolic pathway analysis with an aim of identifying therapeutic targets in mycoplasma hominis ... | 2014 | 25574133 |
| [the comparison of tests for qualitative evaluation of ureaplasma parvum, mycoplasma hominis: "mycoplasma duo", "ureaplasma microtest", "mycoplasma microtest" and "amplisens-florocenosis-mycoplasma-fl"]. | the genital mycoplasma is an opportunistic bacteria and its detection is to be implemented in qualitative format. the study was organized to compare reagents kits "mycoplasma duo", "ureaplasma microtest", "mycoplasma microtest" and "amplisens-florocenosis-mycoplasma-fl". the study resulted in high indicators of diagnostic sensitivity and diagnostic specificity for all kits. at that, the lowest indicators were registered under application of "mycoplasma duo" kit. the study reveled correlation of ... | 2014 | 25552055 |
| the resistance analysis of ureaplasma urealyticum and mycoplasma hominis in female reproductive tract specimens. | the aim of this study was to analyze the drug resistance of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) in female reproductive track from 2007 to 2011 in hangzhou. antibiotics sensitivity test in mycoplasma, which was isolated in clinics from 2007 to 2011 were analyzed retrospectively. the detection of mycoplasma during 2007-2011 was 20,146 (54.37 %), of which the single infection rate of uu was 42.08 %, of mh 1.26 %, and of uu+mh was 11.02 %. the drug resistance rate of uu was incre ... | 2014 | 23749559 |
| bacteria and endotoxin in meconium-stained amniotic fluid at term: could intra-amniotic infection cause meconium passage? | meconium-stained amniotic fluid (msaf) is a common occurrence among women in spontaneous labor at term, and has been associated with adverse outcomes in both mother and neonate. msaf is a risk factor for microbial invasion of the amniotic cavity (miac) and preterm birth among women with preterm labor and intact membranes. we now report the frequency of miac and the presence of bacterial endotoxin in the amniotic fluid of patients with msaf at term. | 2014 | 24028637 |
| co-infection with vaginal ureaplasma urealyticum and mycoplasma hominis increases adverse pregnancy outcomes in patients with preterm labor or preterm premature rupture of membranes. | the purpose of this study was to determine the prevalence of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) in patients with preterm labor or preterm premature rupture of membranes (pprom) and to determine the effect of these organisms on pregnancy outcomes based on the density of colonization. | 2014 | 23796000 |
| in vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against mycoplasma hominis clinical isolates. | the aim of this study was to evaluate the antimicrobial effects of five natural substances against 50 clinical isolates of mycoplasma hominis. | 2014 | 23128812 |
| [unusual forms of persistence of mycoplasma hominis in organism of infected humans]. | study previously unknown forms of persistence of mycoplasma hominis in host organism. | 2015 | 22937717 |
| a new case of mycoplasma hominis mediastinitis and sternal osteitis after cardiac surgery. | we report a case of nosocomial mediastinitis and sternal osteitis due to m. hominis after open-heart surgery in an immuno-competent patient. this infection has been diagnosed by incubating the culture media for an extended period of time, and sequencing 16s rdna directly from the clinical samples. | 2015 | 25532483 |
| an 8-month history of meningitis in an extremely low birth weight infant? - long-lasting infection with ureaplasma parvum. | ureaplasma spp. have been implicated in the pathogenesis of both preterm labor and neonatal morbidity including pneumonia and sepsis and the development of chronic lung disease of prematurity. data on ureaplasma meningitis are limited and partly controversially discussed. | 2015 | 25525814 |
| autoinfection as a cause of postpartum subdural empyema due to mycoplasma hominis. | mycoplasma hominis is a commensal of the genitourinary tract, which is infrequently associated with urogenital infections. extra-urogenital infections due to m. hominis are rare. here, we report an unusual case of m. hominis subdural empyema in a woman occurring shortly after delivery. the patient presented with symptoms suggestive of bacterial meningitis. spinal imaging revealed a subdural empyema that required neurosurgical intervention. cultures from intraoperatively obtained biopsies identif ... | 2015 | 25491170 |
| mycoplasma hominis ssp. associated endocarditis with myocardial necrosis in an alpaca (vicugna pacos) in manitoba in 2011. | severe endocarditis with myonecrosis, moderate to severe pleural and pericardial effusions, and mild ascites were found on necropsy in 3 alpacas. mycoplasma hominis ssp. was detected on polymerase chain reaction (pcr) of fresh affected endocardial tissue in 1 alpaca. | 2015 | 25694661 |
| identification of a gene in mycoplasma hominis associated with preterm birth and microbial burden in intraamniotic infection. | microbial invasion of the amniotic cavity is associated with spontaneous preterm labor and adverse pregnancy outcome, and mycoplasma hominis often is present. however, the pathogenic process by which m hominis invades the amniotic cavity and gestational tissues, often resulting in chorioamnionitis and preterm birth, remains unknown. we hypothesized that strains of m hominis vary genetically with regards to their potential to invade and colonize the amniotic cavity and placenta. | 2015 | 25637842 |
| epidemiology of mycoplasma acquisition in male hiv-1 infected patients: a multistage cross-sectional survey in jiangsu, china. | mycoplasma infections are most frequently associated with disease in the urogenital or respiratory tracts and, in most cases, mycoplasmas infect the host persistently. in hiv-infected individuals the prevalence and role of genital mycoplasmas has not been well studied. to investigate the six species of mycoplasma and the risk factors for infection in jiangsu province, first-void urine and venous blood samples were collected and epidemiological questionnaires were administered after informed cons ... | 2015 | 25792346 |
| in vitro antimicrobial susceptibility of mycoplasma hominis genital isolates. | 2015 | 25791859 | |
| culture-negative endocarditis: mycoplasma hominis infection. | 2015 | 26481285 | |
| [persistence of mycoplasma during urolithiasis]. | study the frequency of detection of mycoplasma and ureaplasma in clinical material from urolithiasis patients. | 2015 | 26470428 |
| antibiotic susceptibility and sequence type distribution of ureaplasma species isolated from genital samples in switzerland. | antibiotic resistance in ureaplasma urealyticum/ureaplasma parvum and mycoplasma hominis is an issue of increasing importance. however, data regarding the susceptibility and, more importantly, the clonality of these organisms are limited. we analyzed 140 genital samples obtained in bern, switzerland, in 2014. identification and antimicrobial susceptibility tests were performed by using the mycoplasma ist 2 kit and sequencing of 16s rrna genes. mics for ciprofloxacin and azithromycin were obtaine ... | 2015 | 26195516 |
| an excision-competent and exogenous mosaic transposon harbors the tetm gene in multiple mycoplasma hominis lineages. | 2015 | 26195506 | |
| [microbial communities on kidney stones]. | the clinical material obtained surgically in patients with kidney stone disease (ksd) was tested for content of the stone microflora using pcr and standard microbiological methods. it was demonstrated that about 50% of stones in patients with ksd were infected with various infection agents as observed using standard microbiological and molecular genetic methods. the percentage of detection of the mycoplasma hominis using cultural method is lower than the percentage detected using pcr, which is d ... | 2015 | 26182663 |
| high rates of double-stranded rna viruses and mycoplasma hominis in trichomonas vaginalis clinical isolates in south brazil. | trichomonas vaginalis is the etiological agent of trichomoniasis, the most common non-viral sexually transmitted disease (std) in world, with 276.4 million new cases each year. t. vaginalis can be naturally infected with mycoplasma hominis and trichomonasvirus species. this study aimed to evaluate the prevalence of t. vaginalis infected with four distinct t. vaginalis viruses (tvvs) and m. hominis among isolates from patients in porto alegre city, south brazil. an additional goal of this study w ... | 2015 | 26160539 |
| complete genome sequence of mycoplasma hominis strain sprott (atcc 33131), isolated from a patient with nongonococcal urethritis. | presented here is the complete and annotated genome sequence of mycoplasma hominis sprott (atcc 33131). the chromosome comprises 695,214 bp, which is approximately 30 kb larger than the syntenic genome of m. hominis pg21(t). tetracycline resistance of strain sprott is most probably conferred by the tetm determinant, harbored on a mosaic transposon-like structure. | 2015 | 26159538 |
| mycoplasma hominis, a rare but true cause of infective endocarditis. | mycoplasma spp. are rarely recognized agents of infective endocarditis. we report a case of mycoplasma hominis prosthetic valve endocarditis diagnosed by 16s ribosomal dna (rdna) pcr and culture of valves in a 74-year-old man. we reviewed the literature and found only 8 other cases reported. | 2015 | 26135868 |
| vacuum-assisted closure (vac)-instill(®) with continuous irrigation for the treatment of mycoplasma hominis mediastinitis. | a 56-year-old patient who underwent ascending aorta replacement postoperatively developed mediastinitis with atypical mycoplasma hominis. we present the first successful treatment of m. hominis mediastinitis after cardiac surgery with vacuum-assisted closure (vac)-instill(®) therapy combined with dilute antiseptic irrigation for bacterial eradication. | 2015 | 24684727 |
| antimicrobial susceptibility patterns of ureaplasma urealyticum and mycoplasma hominis isolated from pregnant women. | mycoplasma hominis and ureaplasma urealyticum bring with them an increased risk of pregnancy complications, such as premature membrane rupture, vaginitis and preterm birth. | 2015 | 26756001 |
| prevalence of ureaplasma urealyticum and mycoplasma hominis in high vaginal swab samples of infertile females. | mycoplasma hominis and ureaplasma urealyticum are important causative agents of vaginitis, cervicitis, postpartum sepsis, reproductive infections and infertility in both males and females. | 2015 | 26756000 |
| development and clinical application of an invaderplus® assay for the detection of genital mycoplasmas. | we developed a pcr-based assay involving invader® technology for detection of the genital mycoplasmas of mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum. we compared its performance with that of a pcr-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (fvu) specimens from men with non-gonococcal urethritis. the tests targeting each of the genital mycoplasmas were specific for the res ... | 2015 | 25892209 |
| mycoplasma and ureaplasma infection and male infertility: a systematic review and meta-analysis. | the relationship between mycoplasma and ureaplasma infection and male infertility has been studied widely; however, results remain controversial. this meta-analysis investigated the association between genital ureaplasmas (ureaplasma urealyticum, ureaplasma parvum) and mycoplasmas (mycoplasma hominis, mycoplasma genitalium), and risk of male infertility. differences in prevalence of ureaplasma and mycoplasma infection between china and the rest of the world were also compared. study data were co ... | 2015 | 26311339 |
| vaginal microbiome and metabolome highlight specific signatures of bacterial vaginosis. | in this study, we sought to find novel bacterial and metabolic hallmarks for bacterial vaginosis (bv). we studied the vaginal microbiome and metabolome of vaginal fluids from bv-affected patients (n = 43) and healthy controls (n = 37) by means of an integrated approach based on quantitative polymerase chain reaction (qpcr) and proton nuclear magnetic resonance ((1)h-nmr). the correlations between the clinical condition and vaginal bacterial communities were investigated by principal component an ... | 2015 | 26385347 |
| simultaneous detection of mycoplasma pneumoniae, mycoplasma hominis and mycoplasma arthritidis in synovial fluid of patients with rheumatoid arthritis by multiplex pcr. | it has been recognized that infectious agents, such as different bacteria and viruses, may play a role in the developing of rheumatoid arthritis (ra). recently, the mycoplasma species has been implicated in the pathogenesis of ra. | 2015 | 26058928 |
| analysis of the complete mycoplasma hominis lbd-4 genome sequence reveals strain-variable prophage insertion and distinctive repeat-containing surface protein arrangements. | the complete genome sequence of mycoplasma hominis lbd-4 has been determined and the gene content ascribed. the 715,165-bp chromosome contains 620 genes, including 14 carried by a strain-variable prophage genome related to mycoplasma fermentans mfv-1 and mycoplasma arthritidis mav-1. comparative analysis with the genome of m. hominis pg21(t) reveals distinctive arrangements of repeat-containing surface proteins. | 2015 | 25720686 |
| effect of microorganisms on etiology of hematospermia. | hematospermia is the presence of blood in the ejaculate. its etiology is congenital, inflammatory, infectious, obstructive, tumoral, vascular, traumatic, iatrogenic or related to systemic disease. it is a quite irritating and life-quality-disturbing condition for men. it is significant to demonstrate infectious-based hematospermia due to the fact that its treatment is easier than for other reasons. | 2015 | 25847903 |
| severe pelvic abscess formation following caesarean section. | we report a case of a 24-year-old woman with severe pelvic abscess formation 2 weeks after secondary caesarean section. the isolated pathogens were a mixture of gardnerella vaginalis, mycoplasma hominis and ureaplasma urealyticum. after initial resistance to systemic antibiotic treatment, she underwent radiologically-guided drainage of the abscesses, whereon she had a continuous recovery. | 2015 | 25911355 |
| ethnic variations in cervical cytokine concentrations and vaginal flora during pregnancy. | pregnancy-mediated changes in immunity may influence risk of hiv-1 acquisition. this risk appears greatest among non-caucasian women. | 2015 | 25041358 |
| clinical and anamnestic characteristics of development of antisperm immunity in infertile men. | the aim of the research was to establish connection between the roles of infertility duration, patient age, various extragenital and genital pathologies in asa pathogenesis. the work is based on retrospective analysis of 496 outpatient cards, which covered detailed anamnestic data and results of past complex clinico-laboratory tests. criteria for inclusion in the study was infertility during marriage for at least 18 month, high concentration of asa-s in booth blood and sperm. it was found that t ... | 2015 | 26355309 |
| distribution of human papillomavirus (hpv) genotypes and bacterial vaginosis presence in cervical samples from paraguayan indigenous. | to determine the frequency of human papillomavirus (hpv) types and to assess bacterial vaginosis (bv) possible associations with cervical infections in indigenous paraguayan women of the department of presidente hayes. | 2015 | 26283550 |
| [dynamics of changes in the numerical density of placental macrophages during urogenital infection in early pregnancy]. | the changes in the numerical density of macrophages of maternal (basal decidua) and fetal (kashchenko-hofbauer cells) origin were studied in the placenta of women with opportunistic (ureaplasma urealyticum and mycoplasma hominis) and pathogenic (chlamydia trachomatis) urogenital microflora. histological study of placenta was performed and cd68-immunoreactive cells were detected immunohistochemically in the basal decidua and in the chorionic villi obtained during artificial abortions for non-medi ... | 2015 | 26234041 |
| intraamniotic inflammation in women with preterm prelabor rupture of membranes. | to characterize subgroups of preterm prelabor rupture of membranes (pprom) and short-term neonatal outcomes based on the presence and absence of intraamniotic inflammation (iai) and/or microbial invasion of the amniotic cavity (miac). | 2015 | 26208287 |
| detection of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis in infertile bulgarian men with multiplex real-time polymerase chain reaction. | the effect of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis over the sperm quality is still unclear. the aim of this study was to determine their prevalence in infertile bulgarian men. a total of 281 men were examined by applying mrt-pcr. the registered prevalence was as follows: c. trachomatis - 13.9%, u. urealyticum - 19.2%, m. hominis - 9.9%. co-infection was established in eight swabs. this first in bulgaria to study for detection of chlamydia and mycoplasmas in infert ... | 2015 | 26010780 |
| analytical performance of multiplex real-time pcr for six sexually transmitted pathogens. | most organisms that cause sexual transmitted diseases (stds) are fastidious pathogens that are difficult to detect with conventional microbiological methods and the proportions of multiple infections were noted up to 39.3% among the sti-positive subjects. however, only a few multiplex pcr and multiplex real-time pcr tests that can screen more than six microorganisms that cause stds have been assessed. | 2015 | 26732001 |
| advances in the understanding and treatment of male urethritis. | neisseria gonorrhoeae and chlamydia trachomatis are well-documented urethral pathogens, and the literature supporting mycoplasma genitalium as an etiology of urethritis is growing. trichomonas vaginalis and viral pathogens (herpes simplex virus types 1 and 2 and adenovirus) can cause urethritis, particularly in specific subpopulations. new data are emerging regarding the potential role of bacterial vaginosis-associated bacteria in urethritis, although results are inconsistent regarding the patho ... | 2015 | 26602615 |
| performance evaluation of a dna chip assay in the identification of major genitourinary pathogens. | to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections. | 2015 | 25533217 |
| performance evaluation of two microbial transport media designed for preservation and transport of chlamydiae, mycoplasma and ureaplasma. | the ability of a non-propagating transport device (test device) to maintain the viability of clinically relevant bacteria was compared with a similar commercial device (predicate device) to establish performance equivalence. test bacteria, namely chlamydia trachomatis, chlamydia pneumoniae, mycoplasma hominis, mycoplasma pneumoniae and ureaplasma urealyticum, were inoculated into the test [puritan medical products universal transport system (unitranz-rt(tm))] and predicate (bd universal viral tr ... | 2015 | 25713205 |
| high atopobium vaginae and gardnerella vaginalis vaginal loads are associated with preterm birth. | bacterial vaginosis is a risk factor for preterm birth. the various conventional methods for its diagnosis are laborious and not easily reproducible. molecular quantification methods have been reported recently, but the specific risk factors they might identify remain unclear. | 2015 | 25452591 |
| in vitro antibacterial activity of azd0914 against human mycoplasmas and ureaplasmas. | in this study, susceptibilities were determined for azd0914, a spiropyrimidinetrione dna gyrase inhibitor, azithromycin, doxycycline, and levofloxacin against mycoplasma and ureaplasma species. the activity of azd0914 was comparable to that of levofloxacin and doxycycline against mycoplasma genitalium and mycoplasma pneumoniae. the azd0914 mic90 against mycoplasma hominis was 8-fold greater than that for levofloxacin. the azd0914 mic90 against ureaplasma species was 4-fold less than that for azi ... | 2015 | 25824220 |
| in vitro evaluation of the comprehensive antimicrobial and antioxidant properties of curtisia dentata (burm.f) c.a. sm: toxicological effect on the human embryonic kidney (hek293) and human hepatocellular carcinoma (hepg2) cell lines. | curtisia dentata is used in african traditional medicine to treat variety of infections. c. dentata leaves were collected from buffelskloof nature reserve, south africa. the ethanol, chloroform, ethyl acetate and acetone extracts were evaluated for antimicrobial activity using micro dilution assay against escherichia coli, pseudomonas aeruginosa, mycobacterium smegmatis, mycoplasma hominis, candida albicans and some clinical isolates of moraxella catarrhalis, proteus mirabilis and staphylococcus ... | 2015 | 27065768 |
| [ribonucleolytic activity of mycoplasmas]. | mycoplasmas are incapable of de novo synthesis of nucleotides and must therefore secrete nucleases in order to replenish the pool of nucleic acid precursors. the nucleolytic activity of mycoplasmas is an important factor in their pathogenicity. bacterial ribonucleases (rnases) may produce a broad spectrum of biological effects, including antiviral and antitumor activity. mycoplasma rnases are therefore of interest. in the present work, capacity of acholeplasma laidlawii and mycoplasma hominis fo ... | 2015 | 25844442 |
| sensitive detection of thirteen bacterial vaginosis-associated agents using multiplex polymerase chain reaction. | bacterial vaginosis (bv) is characterized by a polymicrobial proliferation of anaerobic bacteria and depletion of lactobacilli, which are components of natural vaginal microbiota. currently, there are limited conventional methods for bv diagnosis, and these methods are time-consuming, expensive, and rarely allow for the detection of more than one agent simultaneously. therefore, we conceived and validated a multiplex polymerase chain reaction (m-pcr) assay for the simultaneous screening of thirt ... | 2015 | 26078959 |
| prevalence of mycoplasma genitalium and mycoplasma hominis in urogenital tract of brazilian women. | the role of mycoplasma hominis and m. genitalium in urogenital tract infections remains unknown. furthermore these mollicutes present a complex relationship with the host immune response. the role of inflammatory cytokines in infections also makes them good candidates to investigate bacterial vaginosis and mycoplasma genital infections. therefore, the aim of this study was to detect the above-mentioned mollicutes by quantitative polymerase chain reaction (qpcr) methodologies in vaginal swabs and ... | 2015 | 25886914 |
| polymerase chain reaction as a diagnostic tool for six sexually transmitted infections - preliminary results. | sexually transmitted infections are a very frequent and under-diagnosed cause of illness worldwide. a high number of detection methods and a large range of specimens in which sexually transmitted infections can be determined are available at the moment. polymerase chain reaction performed on first void urine offers the advantage of being non-invasive, self-collectable and has high sensitivity and specificity. we looked to determine the frequency of chlamydia trachomatis, neisseria gonorrhoeae, t ... | 2015 | 26528045 |
| dna microarray characterization of pathogens associated with sexually transmitted diseases. | this study established a multiplex pcr-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (stds)-associated pathogens including neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma, herpes simplex virus (hsv) types 1 and 2, and human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, 35, 39, 54 and 58. the target genes are 16s rrna gene for n. gonorrhoeae, m. genitalium, m. hominism, and ureaplasma, the majo ... | 2015 | 26208181 |
| antibiotic resistance in prevalent bacterial and protozoan sexually transmitted infections. | the emergence of multi-drug resistant sexually transmitted infections (stis) is causing a treatment crisis across the globe. while cephalosporin-resistant gonorrhea is one of the most pressing issues, extensively antibiotic resistant chlamydia trachomatis and mycoplasma hominis are also becoming commonplace. experts have suggested that the failure of current treatment regimens are "largely inevitable" and have called for entirely new classes of antimicrobial agents. with the exception of several ... | 2015 | 26392647 |
| evaluation of the broad-range pcr/esi-ms technology in blood specimens for the molecular diagnosis of bloodstream infections. | rapid identification of the etiological agent in bloodstream infections is of vital importance for the early administration of the most appropriate antibiotic therapy. molecular methods may offer an advantage to current culture-based microbiological diagnosis. the goal of this study was to evaluate the performance of iridica, a platform based on universal genetic amplification followed by mass spectrometry (pcr/esi-ms) for the molecular diagnosis of sepsis-related pathogens directly from the pat ... | 2015 | 26474394 |
| the novel aminomethylcycline omadacycline has high specificity for the primary tetracycline-binding site on the bacterial ribosome. | omadacycline is an aminomethylcycline antibiotic with potent activity against many gram-positive and gram-negative pathogens, including strains carrying the major efflux and ribosome protection resistance determinants. this makes it a promising candidate for therapy of severe infectious diseases. omadacycline inhibits bacterial protein biosynthesis and competes with tetracycline for binding to the ribosome. its interactions with the 70s ribosome were, therefore, analyzed in great detail and comp ... | 2016 | 27669321 |
| prevalence of trichomonas vaginalis in women visiting 2 obstetrics and gynecology clinics in daegu, south korea. | this study explored epidemiological trends in trichomoniasis in daegu, south korea. wet mount microscopy, pcr, and multiplex pcr were used to test for trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in daegu. of the 621 women tested, microscopy detected t. vaginalis in 4 (0.6%) patients, pcr detected t. vaginalis in 19 (3.0%) patients, and multiplex pcr detected t. vaginalis in 12 (1.9%) patients. testing via pcr demonstrated high sensitivity and high neg ... | 2016 | 26951983 |
| bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. | bacterial vaginosis (bv) is a common vaginal disorder in women of reproductive age. since the initial work of leopoldo in 1953 and gardner and dukes in 1955, researchers have not been able to identify the causative etiologic agent of bv. there is increasing evidence, however, that bv occurs when lactobacillus spp., the predominant species in healthy vaginal flora, are replaced by anaerobic bacteria, such as gardenella vaginalis, mobiluncus curtisii, m. mulieris, other anaerobic bacteria and/or m ... | 2016 | 26877884 |
| screening for abnormal vaginal microflora by self-assessed vaginal ph does not enable detection of sexually transmitted infections in ugandan women. | is self-assessed vaginal ph measurement to detect abnormal vaginal bacterial microflora (avf) an adequate prescreening method for detection of genital sexually transmitted infections (stis)? | 2016 | 27112831 |
| [molecular detection of sexually transmitted agents in a symptomatic group of men and its relationship with sexual behavior]. | sexually transmitted infections (stis) affect sexual and reproductive health of millions of men. pathogens such as human papillomavirus (hpv), herpes simplex virus type 1 and 2 (hsv-1 y hsv-2), chlamydia trachomatis,mycoplasmagenitalium,mycoplasma hominis and ureaplasma urealyticum are associated with stis. | 2016 | 28112332 |
| prevalence and distribution of chlamydia trachomatis genovars in indian infertile patients: a pilot study. | to determine the prevalence and distribution of chlamydia trachomatis genovars in patients with infertility by pcr-rflp and ompa gene sequencing. prevalence of other etiological agents (viz., ureaplasma spp. and mycoplasma hominis) were also assessed. endocervical swabs were collected from 477 women and urine was collected from 151 men attending the infertility clinic. the samples were screened for c. trachomatis by cryptic plasmid, ompa gene and nested ompa gene pcr. genotyping was performed by ... | 2016 | 27859690 |
| urethral inflammatory response to ureaplasma is significantly lower than to mycoplasma genitalium and chlamydia trachomatis. | a non-syndromic approach to treatment of people with non-gonococcal urethritis (ngu) requires identification of pathogens and understanding of the role of those pathogens in causing disease. the most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of mycoplasmataceae, in particular ureaplasma urealyticum and ureaplasma parvum to better understand the role of these ureaplasma species in ngu, we have performed a prospective analysis of ... | 2016 | 27558163 |
| gardnerella vaginalis and mollicute detection in rectal swabs from men who have sex with men. | the numbers of rectal sexually transmitted infections are on the rise especially among men who have sex with men. males from men who have sex with men population are encouraged to send a rectal swab to the laboratory for sexually transmitted infection screening at their visit to the genitourinary medicine clinic. in healthy asymptomatic males, the range of pathogens tested is limited therefore other pathogens may be left untreated allowing infections to persist among sexual partners. molecular t ... | 2016 | 27542698 |
| prevalence and antimicrobial resistance of mycoplasmas and chlamydiae in patients with genital tract infections in shanghai, china. | the infections of mycoplasmas and chlamydiae are still severe in patients with genital tract diseases and antimicrobial resistance for these organisms has been changing in recent years. in this study, we reported the prevalence status of ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis in 965 patients with genital tract infection in shanghai from january 2011 to december 2014 and analyzed the antimicrobial resistance of u. urealyticum and m. hominis to 12 kinds of antimicrobi ... | 2016 | 27324895 |
| ureaplasma parvum genotype, combined vaginal colonisation with candida albicans, and spontaneous preterm birth in an australian cohort of pregnant women. | detection of ureaplasma, mycoplasma and candida spp. in the vagina during pregnancy has previously been associated with preterm birth (ptb). however, the prevalence of these microorganisms and the associated obstetric risks (likely to be population-specific) have not been determined in australian women; furthermore, in the case of ureaplasma spp., very few studies have attempted characterisation at the species level and none have examined genotype/serovar status to further refine risk assessment ... | 2016 | 27756249 |
| a prospective observational study of vulvovagintis in pregnant women in argentina, with special reference to candidiasis. | to evaluate the frequency of yeast, bacteria or protozoa in pregnant women and to correlate the possible associations of these microorganisms and their relationships with vulvovaginitis (vv) and cervicitis. vaginal specimens were collected and prepared for smears in microscope slides for the evaluation of yeast, trichomonas vaginalis and bacteria. samples were cultured in specific culture medium. cervical specimens were used to investigate the presence of neisseria gonorrhoeae, chlamydia trachom ... | 2016 | 26931504 |
| cervicitis aetiology and case definition: a study in australian women attending sexually transmitted infection clinics. | studies examining cervicitis aetiology and prevalence lack comparability due to varying criteria for cervicitis. we aimed to outline cervicitis associations and suggest a best case definition. | 2016 | 26586777 |
| neonatal outcomes in subgroups of women with preterm prelabor rupture of membranes before 34 weeks. | to evaluate the influence of microbial invasion of the amniotic cavity (miac) and histological chorioamnionitis (hca) on short-term neonatal outcome in women with preterm prelabor rupture of membranes before 34 weeks of gestation. | 2016 | 26507934 |
| usefulness of a novel multiplex real-time pcr assay for the diagnosis of sexually-transmitted infections. | sexually transmitted infections (sti) are currently on the increase worldwide. new molecular tools have been developed in the past few years in order to improve their diagnosis. an evaluation was carried out using a new commercially available real-time pcr assay, anyplex™ ii sti-7 (seegene, seoul, korea), which detects seven major pathogens in a single reaction - chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum ... | 2016 | 26706392 |
| detection of hpv and co-infecting pathogens in healthy italian women by multiplex real-time pcr. | several pathogens can be transmitted sexually and are an important cause of morbidity among sexually active women. the aim of the study was to detect the presence of human papillomavirus (hpv), chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), trichomonas vaginalis (tv), mycoplasma hominis (mh), mycoplasma genitalium (mg), ureaplasma urealyticum (uu), and ureaplasma parvum (up) in a group of 309 healthy women enrolled at the san camillo - forlanini hospital of rome by using two multiplex r ... | 2016 | 27031891 |
| the first report: an analysis of bacterial flora of the first voided urine specimens of patients with male urethritis using the 16s ribosomal rna gene-based clone library method. | to analyse the bacterial flora of urine from patients with male urethritis using the clone library method. | 2016 | 27013259 |
| maternal serum c-reactive protein in women with preterm prelabor rupture of membranes. | this study evaluated maternal c-reactive protein (crp) as a predictor of microbial invasion of the amniotic cavity (miac) and histological chorioamnionitis (hca) in women with preterm prelabor rupture of the membranes (pprom) before and after 32 weeks of gestation. | 2016 | 26942752 |
| prevalence of chlamydia trachomatis, ureaplasma spp., mycoplasma genitalium and mycoplasma hominis among outpatients in central greece: absence of tetracycline resistance gene tet(m) over a 4-year period study. | a total of 301 men and women attending local urologists and gynaecologists in the state of thessaly, central greece, were tested for chlamydia trachomatis, ureaplasma spp., mycoplasma genitalium and mycoplasma hominis dna. investigation of the tet(m) gene, which confers tetracycline resistance in these genera, was also performed. low incidence of c. trachomatis and mycoplasma spp. as well as high prevalence of ureaplasma spp., especially among women, were found. the tet(m) gene was absent in all ... | 2016 | 26862428 |
| mycoplasma hominis and gardnerella vaginalis display a significant synergistic relationship in bacterial vaginosis. | gardnerella vaginalis plays an important role in bacterial vaginosis (bv,) while the role of genital mollicutes is less obvious. the diagnosis of bv by use of the current gram stain nugent score is also suboptimal for defining the role of mollicutes that lack a cell wall. since bacterial load and diversity is an important prerequisite for bv, real-time quantitative polymerase chain reaction (qpcr) assays enable these to be assessed. the purpose of this study was to define the role of genital mol ... | 2016 | 26796553 |
| the common vaginal commensal bacterium ureaplasma parvum is associated with chorioamnionitis in extreme preterm labor. | to assess the association of vaginal commensal and low-grade pathogenic bacteria including ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, group b streptococcus (gbs), and gardnerella vaginalis, in women who delivered preterm at less than 37-week gestation in the presence or absence of inflammation of the chorioamnionitic membranes. | 2016 | 26795404 |
| combinations and loads of bacteria affect the cytokine production by fetal membranes: an in vitro study. | the polybacterial invasion and inflammation of the amniotic cavity is a common scenario in ptb, and then, we analyzed the cytokine production by human fetal membranes to better understand the host response to polybacterial infections. | 2016 | 27870156 |
| prevalence and antibiotic susceptibility of mycoplasma hominis and ureaplasma urealyticum in genital samples collected over 6 years at a serbian university hospital. | mycoplasma hominis and ureaplasma urealyticum are implicated in a wide array of infectious diseases in adults and children. since some species have innate or acquired resistance to certain types of antibiotics, antibiotic susceptibility testing of mycoplasma isolated from the urogenital tract assumes increasing importance. | 2016 | 26728808 |
| correction for gagneux-brunon et al., mycoplasma hominis, a rare but true cause of infective endocarditis. | 2016 | 26719585 | |
| a disseminated mycoplasma hominis infection in a patient with an underlying defect in humoral immunity. | non-urogenital mycoplasma hominis infections are rare, but may cause life-threatening complications. we describe a case of disseminated m. hominis infection with extensive abscess formation in an immunocompromised patient with iatrogenic hypogammaglobulinemia under rituximab treatment. | 2016 | 26546371 |
| ureaplasma urealyticum and mycoplasma hominis infections and semen quality in 19,098 infertile men in china. | this study aimed to determine the incidence of ureaplasma urealyticum and mycoplasma hominis infections in infertile and fertile men and to investigate their effects on the semen quality. the study also aimed to analyze the drug susceptibility of uu and mh to provide guidance for reasonable antibiotic use. | 2016 | 26537883 |
| mycoplasma hominis induces mediastinitis after a tonsillar abscess. | mycoplasma hominis is commonly involved in genitourinary tract infections. we report a 59-year-old man who developed a m. hominis-associated mediastinitis following acute tonsillar infection. | 2016 | 27957362 |
| genomic determination of minimum multi-locus sequence typing schemas to represent the genomic phylogeny of mycoplasma hominis. | mycoplasma hominis is an opportunistic human pathogen, associated with clinically diverse disease. currently, there is no standardised method for typing m. hominis, which would aid in understanding pathogen epidemiology and transmission. due to availability and costs of whole genome sequencing and the challenges in obtaining adequate m. hominis dna, the use of whole genome sequence analysis to provide clinical guidance is unpractical for this bacterial species as well as other fastidious organis ... | 2016 | 27881098 |
| prevalence of mycoplasma hominis and ureaplasma urealyticum in women undergoing an initial infertility evaluation. | mycoplasma hominis and ureaplasma urealyticum are potentially pathogenic bacterial species that are frequently isolated from the urogenital tract of women. these pathogens could be responsible for various genitourinary diseases and have been associated with adverse pregnancy outcomes and female fertility problems. the aim of this study was to analyse the presence of m. hominis and u. urealyticum in the cervical canal of uterus of women with and without fertility problems. | 2016 | 28078900 |