Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| alkane hydroxylase homologues in gram-positive strains. | we isolated gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (pcr) with degenerate alkane hydroxylase primers and southern blots that most rhodococcus isolates contain three to five quite divergent homologues of the pseudomonas putida gpo1 alkb gene. two mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkb homologues in the remaining strains. | 2002 | 12460275 |
| rhodococcus equi pneumonia in a mare in turkey. | 2002 | 12463539 | |
| triacylglycerols in prokaryotic microorganisms. | triacylglycerols (tag) are fatty acid triesters of glycerol; there are diverse types of tag with different properties depending on their fatty acid composition. the occurrence of tag as reserve compounds is widespread among eukaryotic organisms such as yeast, fungi, plants and animals, whereas occurrence of tag in bacteria has only rarely been described. however, accumulation of tag seems to be widespread among bacteria belonging to the actinomycetes group, such as species of mycobacterium, stre ... | 2002 | 12466875 |
| continuous desulfurization of dibenzothiophene with rhodococcusrhodochrous igts8 (atcc 53968). | desulfurization of a model fuel system consisting of hexadecane and dibenzothiophene (dbt) by rhodococcus rhodochrous igts8 was demonstrated in a 2-l continuous stirred tank reactor (cstr). the reactor was operated in a semicontinuous and continuous mode with and without recycling of the model fuel. a constant volumetric desulfurization activity a(t), (in mg hbp l(-1) h(-1)) was maintained in the reactor with a feeding strategy of fresh cell suspension based on a first-order decay of the biocata ... | 2002 | 12467453 |
| biocatalytic asymmetric hydrogen transfer. | 2002 | 12491297 | |
| [study on the reclassification of two deposited strains as4.1186 and as4.1187 of nocardia]. | polyphasic evidence for the reclassification of nocardia nostocoides as4.1186 and nocardia salmonicolor var. aurantiaca as4.1187 indicated that strain as4.1186 was closely related to saccharothrix texasensis and strain as4.1187 was very closely related to rhodococcus ruber. binary 16s rdna sequence for the pair as4.1186 and saccharothrix texasensis nrrl b-16134t, and the pair as4.1187 and rhodococcus ruber dsm 43338t exhibited 99.3% and 99.5% similarity, respectively. corresponding dna-dna reass ... | 2002 | 12557341 |
| photodegradation of rdx in aqueous solution: a mechanistic probe for biodegradation with rhodococcus sp. | recently we demonstrated that rhodococcus sp. strain dn22 degraded hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) (1) aerobically via initial denitration followed by ring cleavage. using ul 14c-[rdx] and ring labeled 15n-[rdx] approximately 30% of the energetic chemical mineralized (one c atom) and 64% converted to a dead end product that was tentatively identified as 4-nitro-2,4-diaza-butanal (ohchnch2nhno2). to have further insight into the role of initial denitration on rdx decomposition, we p ... | 2002 | 12523428 |
| clostridium difficile infections in animals with special reference to the horse. a review. | in human medicine, clostridium (c.) difficile is since many years a well-known cause of nosocomial diarrhea induced by antibiotic treatment. in horses, c. difficile was recently suggested as a possible enteric pathogen. the bacterium is associated with acute colitis in mature horses following treatment with antibiotics. c. difficile, and/or its cytotoxin, is also associated with acute colitis in mares when their foals are being treated with erythromycin and rifampicin for rhodococcus equi pneumo ... | 2002 | 12540137 |
| antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients. | the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ... | 2002 | 12458127 |
| rhodococcus globerulus keratitis after laser in situ keratomileusis. | a healthy 20-year-old woman with myopia had uneventful bilateral laser in situ keratomileusis after which the uncorrected visual acuity was 20/20 in the right eye and 20/30 in the left eye. fifteen days later, a stromal paraxial lesion was found in the right eye with a corresponding loss of visual acuity, pain, and photophobia. the flap was lifted and the infiltrate scraped for smears. cultures showed that rhodococcus globerulus was the infectious agent. intensive topical antibiotic treatment wa ... | 2002 | 12498868 |
| rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. | a polyphasic study was undertaken to establish the taxonomic position of a soil isolate that had provisionally been assigned to the genus rhodococcus. the organism showed a combination of phenotypic properties typical of rhodococci and formed a distinct phyletic line within the rhodococcus erythropolis 165 rdna subclade. the organism was readily distinguished from representatives of validly described species classified in this subclade on the basis of dna-dna relatedness and phenotypic data. con ... | 2002 | 12508878 |
| investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland. | wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ... | 2002 | 12512252 |
| pharmacokinetics of azithromycin in foals after i.v. and oral dose and disposition into phagocytes. | the properties of azithromycin suggest that it may be an alternative to erythromycin for treatment of rhodococcus equi pneumonia in foals. to investigate this possibility, the disposition of azithromycin in plasma, polymorphonuclear leukocytes (pmn), and alveolar cells was examined after a single administration in foals. azithromycin suspension was administered orally (p.o.) at a dose of 10 mg/kg to five healthy 2-3-month-old foals. two weeks later, azithromycin for injection was administered by ... | 2002 | 12000529 |
| reductive deamination as a new step in the anaerobic microbial degradation of halogenated anilines. | in this paper we report the isolation and characterization of an anaerobic enrichment culture as well as of a rhodococcus sp. strain 2 capable of degrading 3,4-dihaloanilines under nitrate reducing conditions. using mass spectrometry several of the intermediates formed in the process of 3,4-dichloroaniline conversion were identified. most interesting is the observation of reductive deamination and the formation of 1,2-dichlorobenzene as one of the intermediates. using 19f nmr and fluorinated 3,4 ... | 2002 | 12007823 |
| survival and naphthalene-degrading activity of rhodococcus sp. strain 1bn in soil microcosms. | the survival and activity of rhodococcus sp. strain 1bn, inoculated into naphthalene-contaminated sandy-loam soil microcosms, were studied using classical and molecular methods. | 2002 | 12010546 |
| phages and other mobile virulence elements in gram-positive pathogens. | 2002 | 12012871 | |
| biodegradation of phenol in synthetic and industrial wastewater by rhodococcus erythropolis upv-1 immobilized in an air-stirred reactor with clarifier. | phenol biodegradation by suspended and immobilized cells of rhodococcus erythropolis upv-1 was studied in discontinuous and continuous mode under optimum culture conditions. phenol-acclimated cells were adsorbed on diatomaceous earth, where they grew actively forming a biofilm of short filaments. immobilization protected cells against phenol and resulted in a remarkable enhancement of their respiratory activity and a shorter lag phase preceding active phenol degradation. under optimum operation ... | 2002 | 12021809 |
| water transformation in the media of biofilters controlled by rhodococcus fascians in treating an ethyl acetate-contaminated airstream. | biofilters do not provide much water for bacteria to grow. to use them efficiently and properly, it is essential to understand the kinetics of water transformation and to control moisture levels. this study aims to clarify whether the metabolism of microorganisms will improve the water-holding capacity of media or will intensify drying. this experiment was conducted in duplicate, that is, both with and without bacterial inoculation. both the constant water content mode and the declining water co ... | 2002 | 12022691 |
| [antagonistic interactions between stress factors during the growth of microorganisms under conditions simulating the parameters of their natural ecotopes]. | two stress factors, hypoxia (microaerobic conditions) and a high salt concentration, if applied simultaneously to aerobic microorganisms, display an antagonistic mode of interaction. as a result, the nacl level that is usually optimal for moderate halophiles (5-6%) becomes optimal for the growth of weak halophiles (rhodococcus erythropolis and shewanella sp. cn32); the halotolerant yeast yarrowia lypolytica acquires halophilic properties (with a growth optimum at a nacl concentration of 10%), an ... | 2002 | 12024812 |
| [the role of low-molecular-weight nitrogen compounds in the osmotolerance of rhodococcus erythropolis and arthrobacter globiformis]. | investigations showed that rhodococcus erythropolis e-15 and arthrobacter globiformis 2f cells respond to osmotic shock by increasing the synthesis of free amino acids, primarily glutamic acid (80% of the intracellular free amino acid pool). the osmoprotective role of glutamic acid follows from its beneficial effect on the growth of bacteria in high-salinity media. it was found that the addition of this amino acid to the growth medium at a concentration of 2 mm shortened the lag phase and increa ... | 2002 | 12024813 |
| virulence genes of the phytopathogen rhodococcus fascians show specific spatial and temporal expression patterns during plant infection. | the phytopathogenic bacterium rhodococcus fascians provokes shoot meristem formation and malformations on aerial plant parts, mainly at the axils. the interaction is accompanied by bacterial colonization of the plant surface and tissues. upon infection, the two bacterial loci required for full virulence, fas and att, were expressed only at the sites of symptom development, although their expression profiles differed both spatially and temporally. the att locus was expressed principally in bacter ... | 2002 | 12026179 |
| hydrophobicity development, alkane oxidation, and crude-oil emulsification in a rhodococcus species. | the relationship between the phenomena alkane oxidation, extreme hydrophobicity of the cell surface, and crude-oil emulsification in rhodococcus sp. strain 094 was investigated. compounds that induce the emulsifying ability simultaneously induced the cytochrome p450-containing alkane oxidizing system and the transition from low to high cell-surface hydrophobicity. exposed to inducers of crude-oil emulsification, the cells developed a strong hydrophobic character during exponential growth, which ... | 2002 | 12030701 |
| discrimination and taxonomy of geographically diverse strains of nitrile-metabolizing actinomycetes using chemometric and molecular sequencing techniques. | mycolic acid-containing actinomycetes capable of metabolizing nitriles were recovered from deep-sea sediments and terrestrial soils by enrichment culture on acetonitrile, benzonitrile, succinonitrile or bromoxynil. a total of 43 nitrile-degrading strains were isolated and, together with previously recovered nitrile-degrading rhodococci, were identified by a polyphasic taxonomic approach, which included mycolic acid profiles, pyrolysis mass spectrometry (pyms), genomic fingerprinting based on seq ... | 2002 | 12030852 |
| cavitary lung lesions due to coinfection of rhodococcus equi and mycobacterium kansasii in a patient with acquired immunodeficiency syndrome. | 2002 | 12034423 | |
| 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive rhodococcus opacus 1cp: crystallization and preliminary crystallographic analysis. | 4-chlorocatechol 1,2-dioxygenase (4-clc1,2do) from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, an enzyme involved in the aerobic biodegradation of chloroaromatic compounds, has been crystallized. 4-clc1,2do, which specifically catalyzes the intradiol cleavage of 4-substituted catechols, which are intermediates in the degradation of a variety of aromatic pollutants, to the corresponding maleylacetates, has recently been purified to homogeneity. the enzyme is an homodimer co ... | 2002 | 12037322 |
| degradation of phenol by rhodococcus erythropolis upv-1 immobilized on biolite in a packed-bed reactor. | a strain of rhodococcus erythropolis has been isolated and identified by 16s rrna sequencing. cells acclimated to phenol can be adsorbed on the external surface of beads of the ceramic support biolite where they grow forming a network of large filaments. exponentially-growing cells were adsorbed faster than their stationary-phase counterparts. immobilization resulted in a remarkable enhancement of the respiratory activity of cells and a shorter lag phase preceding the active phenol degradation. ... | 2002 | 12052678 |
| transfer of tsukamurella wratislaviensis goodfellow et a. 1995 to the genus rhodococcus as rhodococcus wratislaviensis comb. nov.. | a polyphasic study was undertaken to clarify the taxonomic position of the type strain (n805t) of tsukamurella wratislaviensis. this organism showed a combination of phenotypic properties, notably chemotaxonomic markers, consistent with its classification in the genus rhodococcus. comparative 16s rdna sequencing studies indicated that strain 805t falls into the rhodococcus erythropolis subclade, where it forms a monophyletic group with the type strains of rhodococcus opacus and rhodococcus perco ... | 2002 | 12054234 |
| substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains i and ii. | hoxn, a high-affinity, nickel-specific permease of ralstonia eutropha h16, and nhlf, a nickel/cobalt permease of rhodococcus rhodochrous j1, are structurally related members of the nickel/cobalt transporter (nicot) family. these transporters have an eight-helix structure and are characterized by highly conserved segments with polar or charged amino acid residues in transmembrane domains (tmds) ii, iii, v, and vi. two histidine residues in a ni2+ binding motif, the signature sequence of nicots, i ... | 2002 | 12057951 |
| rhodococcus equi brain abscess in an immunocompetent patient. | rhodococcus equi brain abscesses usually occur in immunocompromised patients with prolonged and refractory pulmonary infections. herein we report a case of r. equi brain abscess in a 67-y-old man without immunodepression. our patient recovered after neurosurgical resection and prolonged antimicrobial therapy with vancomycin and trimethoprim-sulfamethoxazole. | 2002 | 12064695 |
| h(2)o(2), which causes macrophage-related stress, triggers induction of expression of virulence-associated plasmid determinants in rhodococcus equi. | the response of the intracellular pathogen rhodococcus equi to h(2)o(2) treatment, a situation potentially encountered after the oxidative burst of alveolar macrophages, was analyzed. compared to other bacteria, including deinococcus radiodurans, r. equi showed exceptionally high resistance to this stress. a proteomic approach showed that four polypeptides present in the wild-type strain (85f) are missing in the plasmid-cured strain 85f(p-), and by using a dna macroarray, we identified two plasm ... | 2002 | 12065520 |
| population analysis of a binary bacterial culture by multi-parametric flow cytometry. | to study the degradation of a xenobiotic that requires a mixed culture it is essential to monitor the proportions and to control the population dynamics of the component strains. for these purposes fluorochromising techniques and multi-parametric flow cytometry were used to follow rhodococcus erythropolis k2-3 and ochrobactrum anthropi k2-14, both of which are needed to degrade 4-(2,4-dichlorophenoxy)butyric acid (2,4-db). although the two strains can grow in constant proportions in mixed cultur ... | 2002 | 12067522 |
| a novel lipoarabinomannan from the equine pathogen rhodococcus equi. structure and effect on macrophage cytokine production. | rhodococcus equi is a major cause of foal morbidity and mortality. we have investigated the presence of lipoglycan in this organism as closely related bacteria, notably mycobacterium tuberculosis, produce lipoarabinomannans (lam) that may play multiple roles as virulence determinants. the lipoglycan was structurally characterized by gas chromatography-mass spectrometry following permethylation, capillary electrophoresis after chemical degradation, and (1)h and (31)p and two-dimensional heteronuc ... | 2002 | 12072437 |
| cometabolic ring fission of dibenzofuran by gram-negative and gram-positive biphenyl-utilizing bacteria. | thirty-five strains of soil bacteria were grown with biphenyl (bp) and tested for their capacity to cooxidize dibenzofuran (dbf). during metabolism of dbf, the culture medium of 17 strains changed from colorless to orange, indicating a meta-cleavage pathway of dbf degradation. the ring cleavage product of these isolates was shown to be 2-hydroxy-4-(3'-oxo-3' h-benzofuran-2'-yliden)but-2-enoic acid (hobb). the strain sbug 271, studied in detail and identified as rhodococcus erythropolis, degraded ... | 2002 | 12073133 |
| selection of microbial consortia for treating metal-working fluids. | the aim of this research was to determine the effectiveness of a strategy for constructing microbial consortia for treating chemically mixed industrial effluent, based on a more thorough understanding of communities within waste metal-working fluids (mwfs). complementary phenotypic and genotypic methods revealed that the microbial communities in spent mwfs had low diversity and were very similar in species composition in samples originating from different locations and uses. of 65 bacterial isol ... | 2002 | 12080423 |
| family shuffling of a targeted bpha region to engineer biphenyl dioxygenase. | in this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (bpdos). instead of shuffling the whole gene, we have targeted a fragment of bpha that is critical for enzyme specificity. we also describe a new protocol to screen for more potent bpdos that is based on the detection of catechol metabolites from chlorobiphenyls. several bpha variants with extended potency to degrade polychlorinated biph ... | 2002 | 12081948 |
| identification of a new class of cytochrome p450 from a rhodococcus sp. | a degenerate set of pcr primers were used to clone a gene encoding a cytochrome p450 (the p450rhf gene) from rhodococcus sp. strain ncimb 9784 which is of unique primary structural organization. surprisingly, analysis of the translation product revealed that the p450 is fused to a reductase domain at the c terminus which displays sequence conservation for dioxygenase reductase proteins. the reductase partner comprises flavin mononucleotide- and nadh-binding motifs and a [2fe2s] ferredoxin-like c ... | 2002 | 12081961 |
| monocyclic aromatic hydrocarbon degradation by rhodococcus sp. strain dk17. | rhodococcus sp. strain dk17 was isolated from soil and analyzed for the ability to grow on o-xylene as the sole carbon and energy source. although dk17 cannot grow on m- and p-xylene, it is capable of growth on benzene, phenol, toluene, ethylbenzene, isopropylbenzene, and other alkylbenzene isomers. one uv-generated mutant strain, dk176, simultaneously lost the ability to grow on o-xylene, ethylbenzene, isopropylbenzene, toluene, and benzene, although it could still grow on phenol. the mutant st ... | 2002 | 12089003 |
| cloning and heterologous expression of an enantioselective amidase from rhodococcus erythropolis strain mp50. | the gene for an enantioselective amidase was cloned from rhodococcus erythropolis mp50, which utilizes various aromatic nitriles via a nitrile hydratase/amidase system as nitrogen sources. the gene encoded a protein of 525 amino acids which corresponded to a protein with a molecular mass of 55.5 kda. the deduced complete amino acid sequence showed homology to other enantioselective amidases from different bacterial genera. the nucleotide sequence approximately 2.5 kb upstream and downstream of t ... | 2002 | 12089004 |
| biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene. | using a combined strategy of random mutagenesis of haloalkane dehalogenase and genetic engineering of a chloropropanol-utilizing bacterium, we constructed an organism that is capable of growth on 1,2,3-trichloropropane (tcp). this highly toxic and recalcitrant compound is a waste product generated from the manufacture of the industrial chemical epichlorohydrin. attempts to select and enrich bacterial cultures that can degrade tcp from environmental samples have repeatedly been unsuccessful, proh ... | 2002 | 12089046 |
| increased pulmonary activation of nuclear factor-kappab (nf-kappab) in foals inoculated with rhodococcus equi is associated with increased expression of inflammatory cytokines. | previous studies revealed that foals inoculated with virulent rhodococcus equi had significantly higher pulmonary levels of interleukin-1beta, interleukin-12 p40, interferon-gamma, and tumor necrosis factor-alpha mrna compared to foals inoculated with an avirulent plasmid-cured derivative. the purpose of this study was to determine if the increases in cytokine expression were associated with increased pulmonary activation of nuclear factor-kappab (nf-kappab). electrophoretic mobility shift assay ... | 2002 | 12102205 |
| [rhodococcus equi infection: a rare cause of cutaneous vasculitis]. | 2002 | 12108184 | |
| alkanotrophic rhodococcus ruber as a biosurfactant producer. | in this report we examined the structure and properties of surface-active lipids of rhodococcus ruber. most historical interest has been in the glycolipids of rhodococcus erythropolis, which have been extensively characterised. r. erythropolis has been of interest due to its great metabolic diversity. only recently has the metabolic potential of r. ruber begun to be explored. one major difference in the two species is that most r. ruber strains are able to oxidise the gaseous alkanes propane and ... | 2002 | 12111164 |
| biodesulfurization of benzothiophene and dibenzothiophene by a newly isolated rhodococcus strain. | rhodococcus sp. kt462, which can grow on either benzothiophene (bt) or dibenzothiophene (dbt) as the sole source of sulfur, was newly isolated and characterized. gc and gc-ms analyses revealed that strain kt462 has the same bt desulfurization pathway as that reported for paenibacillus sp. a11-2 and sinorhizobium sp. kt55. the desulfurized product of dbt produced by this strain, as well as other dbt-desulfurizing bacteria such as r. erythropolis ka2-5-1 and r. erythropolis igts8, was 2-hydroxybip ... | 2002 | 12111165 |
| a two-step, one-pot enzymatic synthesis of cephalexin from d-phenylglycine nitrile. | a cascade of two enzymatic transformations is employed in a one-pot synthesis of cephalexin. the nitrile hydratase (from r. rhodochrous mawe)-catalyzed hydration of d-phenylglycine nitrile to the corresponding amide was combined with the penicillin g acylase (penicillin amidohydrolase, e.c. 3.5.1.11)-catalyzed acylation of 7-adca with the in situ-formed amide to afford a two-step, one-pot synthesis of cephalexin. d-phenylglycine nitrile appeared to have a remarkable selective inhibitory effect o ... | 2002 | 12115424 |
| site-directed mutagenesis for cysteine residues of cobalt-containing nitrile hydratase. | three cysteine residues, which are completely conserved among alpha-subunits in all nitrile hydratases, are thought to be the ligands of a metal ion in the catalytic center of this enzyme. these cysteine residues (i.e. alpha c102, alpha c105 and alpha c107) in the high-molecular-mass nitrile hydratase (h-nhase) of rhodococcus rhodochrous j1 were replaced with alanine by site-directed mutagenesis using the r. rhodochrous atcc12674 host-vector system, and the resultant transformants were investiga ... | 2002 | 12121763 |
| rhodococcus equi pulmonary infection in a pancreas-alone transplant recipient: consequence of intense immunosuppression. | we report the case of a pancreas-alone transplant recipient who developed rhodococcus equi pneumonia after receiving multiple courses of antilymphocyte therapy for the treatment of recurrent acute pancreas allograft rejection. we also review and discuss the diagnosis, clinical course, and treatment of 18 cases of r. equi infection reported in solid organ transplant recipients. the lung is the most common primary site of infection, but r. equi infection is difficult to diagnose because of the ple ... | 2002 | 12123426 |
| rhodococcus equi infection in transplant recipients: a case of mistaken identity and review of the literature. | the incidence of rhodococcus equi infection in solid-organ transplant recipients continues to rise throughout the world. unfortunately, this opportunistic pathogen is still underestimated and potentially disregarded by physicians and microbiology laboratories due to its morphology on gram staining. pulmonary involvement is the most common finding in the immunocompromised host. we report a case of a 63-year-old heart-transplant recipient who presented with increasing fatigue and nonproductive cou ... | 2002 | 12123427 |
| rhodococcus equi: pulmonary cavitation lesion in patient infected with hiv cured by levofloxacin and rifampicin. | 2002 | 12131231 | |
| use of both 16s rrna and engineered functional genes with real-time pcr to quantify an engineered, pcb-degrading rhodococcus in soil. | a real-time pcr (rtm-pcr) assay using fluorescently labeled oligonucleotides (taqman probes) was used to detect and quantify the recombinant rhodococcus sp. strain rha1(fcb) in soil. one primer and probe set targeted a hypervariable region of the 16s rrna gene unique to strain rha1(fcb) and its phylogenetic relatives, and the other set targeted the recombinant 4-chlorobenzoate (4-cba) degradation operon (fcb) and was strain-specific. the method had a 6-log dynamic range of detection (10(2)-10(7) ... | 2002 | 12133610 |
| identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with acquired immune deficiency syndrome and prevalence of virulent r. equi in soil collected from domestic animal farms in chiang mai, thailand. | the prevalence of virulent rhodococcus equi in soil collected from 17 domestic animal farms (from 12 cattle, 1 pig, and 4 horse farms) and in 6 clinical specimens from patients with acquired immune deficiency syndrome (aids) in chiang mai, thailand, was investigated. the isolates were tested for the presence of 15-17-kda antigens (vapa) and a 20-kda antigen (vapb) by immunoblotting and for the presence of virulence plasmid dna. rhodococcus equi was isolated from most soil samples (68 of 80) obta ... | 2002 | 12135268 |
| [the efficient accumulation of cesium ions by rhodococcus cells]. | bacteria of the genus rhodococcus were found to be able to accumulate cesium by means of active transport and nonspecific sorption on the cell surface structures. the maximum removal (up to 97%) of cesium from a medium with ammonium acetate was observed at 28 degrees c, ph 7.8-8.6, and an equimolar content (0.2 mm) of potassium and cesium ions in the medium. the most active cesium-accumulating rhodococcus sp. strains can be used for purification of industrial wastewaters contaminated with radion ... | 2002 | 12138767 |
| a novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (dszb), from a dibenzothiophene-desulfurizing bacterium rhodococcus erythropolis ka2-5-1: gene overexpression and enzyme characterization. | dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, is microbially desulfurized to 2-hydroxybiphenyl (2-hbp), and the gene operon dszabc was required for dbt desulfurization. the final step in the microbial dbt desulfurization is the conversion of 2'-hydroxybiphenyl-2-sulfinate (hbpsi) to 2-hbp catalyzed by dszb. in this study, dszb of a dbt-desulfurizing bacterium rhodococcus erythropolis ka2-5-1 was overproduced in escherichia coli by coexpression with chaperonin genes, gr ... | 2002 | 12147352 |
| plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by terrabacter sp. strain yk3. | the genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of pcr primers designed by using conserved sequences of the dioxygenase alpha subunit genes. one sequence of alpha subunit genes was commonly amplified from four dibenzofuran-utilizing actinomycetes: terrabacter sp. strains yk1 and yk3, rhodococcus sp. strain yk2, and microbacterium sp. strain yk18. a 5.2-kb psti fragment encoding the alpha and beta subunits of the terminal diox ... | 2002 | 12147464 |
| biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by rhodococcus sp. strain wu-k2r. | naphtho[2,1-b]thiophene (nth) is an asymmetric structural isomer of dibenzothiophene (dbt), and in addition to dbt derivatives, nth derivatives can also be detected in diesel oil following hydrodesulfurization treatment. rhodococcus sp. strain wu-k2r was newly isolated from soil for its ability to grow in a medium with nth as the sole source of sulfur, and growing cells of wu-k2r degraded 0.27 mm nth within 7 days. wu-k2r could also grow in the medium with nth sulfone, benzothiophene (bth), 3-me ... | 2002 | 12147483 |
| [study on production of acrylamide by microbial method (ii)--enzyme catalytic kinetics and de-active dynamics of nitrile hydratase]. | the hydration reaction by microbial method is the crisis of the procedure of acrylamide production from acrylonitrile. this research studied the enzyme catalytic kinetics and de-active kinetics of nitrile hydratase in the type of free cell. firstly, the effects of the concentration of cells, the temperature, ph value, the concentration of acrylonitrile and the concentration of acrylamide on the activity of nitrile hydratase was investigated. the result is that the temperature and the concentrati ... | 2002 | 12148289 |
| detection of rhodococcus equi by polymerase chain reaction using species-specific nonproprietary primers. | species-specific primers for the polymerase chain reaction (pcr) for the detection of rhodococcus equi were developed. these primers were based on unique dna fragments produced from r. equi reference strains and field isolates. following random amplification of polymorphic dna from r. equi and r. rhodochrous with a set of 40 arbitrary 10-base pair (bp) primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of r. equi chromosomal dna. this pcr product was limi ... | 2002 | 12152820 |
| tolerance of bacteria to organic solvents. | organic-solvent-tolerant bacteria are a relatively novel group of extremophilic microorganisms. they overcome the toxic and destructive effects of organic solvents due to the presence of various adaptive mechanisms. extensive studies done on the toluene tolerance of certain pseudomonas strains have led to an understanding of the mechanisms of organic solvent tolerance involving novel adaptations such as the toluene efflux pumps, cis-trans isomerisation of membrane fatty acids, rapid membrane rep ... | 2002 | 12160316 |
| engineering an indene bioconversion process for the production of cis-aminoindanol: a model system for the production of chiral synthons. | cis-aminoindanol, a key chiral precursor to the hiv protease inhibitor crixivan, can be derived from indene oxidation products of (2r) stereochemistry. a number of different microorganisms, notably strains of the genera pseudomonas and rhodococcus, have been isolated that catalyze the oxygenation of indene to indandiol with greater stereospecificity than is achievable through traditional chemical synthesis. the yield and ultimate optical purity of indandiol produced in such biocatalytic processe ... | 2002 | 12172601 |
| p450(camr), a cytochrome p450 catalysing the stereospecific 6- endo-hydroxylation of (1 r)-(+)-camphor. | rhodococcus sp. ncimb 9784 accumulated 6- endo-hydroxycamphor 3 when grown on (1 r)-(+)-camphor 1 as sole carbon source. the structure of 3 has been unambiguously assigned for the first time using x-ray crystallography. a soluble cytochrome p450 hydroxylase, induced by growth on (1 r)-(+)-camphor and designated p450(camr), has been isolated from the bacterium rhodococcus sp. ncimb 9784. using authentic 6- endo hydroxycamphor as standard, a cell-free system consisting of pure p450(camr) and putid ... | 2002 | 12172608 |
| population dynamics of free-floating and attached bacteria in a styrene-degrading biotrickling filter analyzed by denaturing gradient gel electrophoresis. | population dynamics was studied in a 52-l biotrickling filter (btf) operated for 182 days and used to clean air contaminated with styrene vapors. in the btf, biomass grew either as free-floating (planktonic) or attached (sessile) microorganisms. pcr-amplified 16s rdna fragments from planktonic and sessile cells within the bioreactor were analyzed using denaturing gradient gel electrophoresis (dgge). the results indicated that the complexity of biofilm community was always more pronounced than th ... | 2002 | 12172630 |
| evidence that rdx biodegradation by rhodococcus strain dn22 is plasmid-borne and involves a cytochrome p-450. | to investigate the biodegradation of the explosive compound rdx in rhodococcus strain dn22, a bacterium previously isolated for its ability to grow on rdx as sole nitrogen source. | 2002 | 12174045 |
| the principal determinants for the structure of the substrate-binding pocket are located within a central core of a biphenyl dioxygenase alpha subunit. | protein engineering by segment exchange was used to distinguish between regions of major and minor influence on the structure of the substrate-binding pocket of a biphenyl dioxygenase (bdo). eight chimaeric enzyme systems were generated that each consisted of a hybrid hydroxylase alpha subunit (bpha1) containing segments from burkholderia sp. strain lb400 and rhodococcus globerulus p6, and of a hydroxylase beta subunit (bpha2), a ferredoxin (bpha3) and a ferredoxin reductase (bpha4) from strain ... | 2002 | 12177337 |
| molecular and functional characterization of ksha and kshb, encoding two components of 3-ketosteroid 9alpha-hydroxylase, a class ia monooxygenase, in rhodococcus erythropolis strain sq1. | 9 alpha-hydroxylation of 4-androstene-3,17-dione (ad) and 1,4-androstadiene-3,17-dione (add) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (ksh), a key enzyme in microbial steroid catabolism. very limited knowledge is presently available on the ksh enzyme. here, we report for the first time the identification and molecular characterization of genes encoding ksh activity. the ksha and kshb genes, encoding ksh in rhodococcus erythropolis strain sq1, were cloned by functional complementation of ... | 2002 | 12180920 |
| crystal structures of a meta-cleavage product hydrolase from pseudomonas fluorescens ip01 (cumd) complexed with cleavage products. | 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase (cumd) from pseudomonas fluorescens ip01 hydrolyzes a meta-cleavage product generated in the cumene (isopropylbenzene) degradation pathway. the crystal structures of the inactive s103a mutant of the cumd enzyme complexed with isobutyrate and acetate ions were determined at 1.6 and 2.0 a resolution, respectively. the isobutyrate and acetate ions were located at the same position in the active site, and occupied the site for a part of the hydroly ... | 2002 | 12192074 |
| recombinant rhodococcus sp. strain t09 can desulfurize dbt in the presence of inorganic sulfate. | the putative rhodococcus rrn promoter region was cloned from the benzothiophene desulfurizing rhodococcus sp. strain t09, and the dibenzothiophene desulfurizing gene, dsz, was expressed under the control of the putative rrn promoter in the strain t09 using a rhodococcus-e.coli shuttle vector. strain t09 harboring the expression vector, pnt, could desulfurize dibenzothiophene in the presence of inorganic sulfate, methionine, or cysteine, while the dsz phenotype was completely repressed in recombi ... | 2002 | 12192519 |
| rhamnolipid stimulates uptake of hydrophobic compounds by pseudomonas aeruginosa. | the biodegradation of hexadecane by five biosurfactant-producing bacterial strains (pseudomonas aeruginosa ug2, acinetobacter calcoaceticus rag1, rhodococcus erythropolis dsm 43066, r. erythropolis atcc 19558, and strain bcg112) was determined in the presence and absence of exogenously added biosurfactants. the degradation of hexadecane by p. aeruginosa was stimulated only by the rhamnolipid biosurfactant produced by the same organism. this rhamnolipid did not stimulate the biodegradation of hex ... | 2002 | 12200306 |
| practical and convenient enzymatic synthesis of enantiopure alpha-amino acids and amides. | catalyzed by the nitrile hydratase and the amidease in rhodococcus sp. aj270 cells under very mild conditions, a number of alpha-aryl- and alpha-alkyl-substituted dl-glycine nitriles 1 rapidly underwent a highly enantioselective hydrolysis to afford d-(-)-alpha-amino acid amides 2 and l-(+)-alpha-amino acids 3 in high yields with excellent enantiomeric excesses in most cases. the overall enantioselectivity of the biotransformations of nitriles originated from the combined effects of a high l-ena ... | 2002 | 12201779 |
| fighting foam with phages? | seventeen (17) phages infective for the mycolata were isolated from six samples of activated sludge using 21 prospective hosts from the genera dietzia, gordonia, nocardia, rhodococcus, tsukamurella and mycobacterium. their morphology indicated that they were all members of the viral family siphoviridae, but they varied in the size of the icosahedral head and length of non-contractile tail, suggesting they were different. this was confirmed by host-range studies with 47 strains of mycolata, which ... | 2002 | 12216679 |
| dispelling the "nocardia amarae" myth: a phylogenetic and phenotypic study of mycolic acid-containing actinomycetes isolated from activated sludge foam. | right-angle branched filaments and rods micromanipulated from activated sludge foam and mixed liquor were identified as putatively novel members of the genera gordonia, mycobacterium and rhodococcus using a combination of chemical, molecular and morphological data. pyrolysis mass spectrometric analyses of gordoniae isolated in both the present and a previous study revealed pyro-groups, distinct from validly described gordonia species, which could be equated with those based on morphological prop ... | 2002 | 12216692 |
| role of surgery in rhodococcus equi pulmonary infections. | rhodococcus equi (r. equi) is a soil-dwelling bacterium that is increasingly associated with pulmonary infections in immunocompromised patients. while antibiotic therapy remains the cornerstone of treatment, surgery combined with antibiotics has an important role in select cases. we report two cases of pulmonary r. equi in immunocompromised patients who were treated with antibiotics, followed by surgical resection of the infected tissues, and then long-term antibiotics. both patients had excelle ... | 2002 | 12217735 |
| a new modified ortho cleavage pathway of 3-chlorocatechol degradation by rhodococcus opacus 1cp: genetic and biochemical evidence. | the 4-chloro- and 2,4-dichlorophenol-degrading strain rhodococcus opacus 1cp has previously been shown to acquire, during prolonged adaptation, the ability to mineralize 2-chlorophenol. in addition, homogeneous chlorocatechol 1,2-dioxygenase from 2-chlorophenol-grown biomass has shown relatively high activity towards 3-chlorocatechol. based on sequences of the n terminus and tryptic peptides of this enzyme, degenerate pcr primers were now designed and used for cloning of the respective gene from ... | 2002 | 12218013 |
| characterization of virulence plasmid types in rhodococcus equi isolates from foals, pigs, humans and soil in hungary. | rhodococcus equi isolates (204) obtained from foals (lung abscesses, lymph nodes, nasal discharge, rectal swabs) bred in 15 studs located throughout hungary, isolates from soil samples, lymph nodes of pigs and from lesions of human patients were examined to determine genotypic diversity of virulence-associated plasmids. isolates were tested for the presence of 15-17 kda virulence-associated protein antigen (vapa) and 20k da (vapb) genes by polymerase chain reaction (pcr). plasmid dnas were isola ... | 2002 | 12220812 |
| isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading rhodococcus species. | sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including rhodococcus, microbacterium, and terrabacter genera. heretofore, no dibenzofuran-utilizing strain belonging to the genus microbacterium has been reported. five extradiol dioxygenase genes (dfdb, and edil to 4) of the strain rhodococcus sp. yk2 were cloned and analyzed. the nucleotide sequence of dfdb gene was almost identical to the bphc1 gene of terrabacter sp. dpo360, which wa ... | 2002 | 12224629 |
| flavin reductase coupling with two monooxygenases involved in dibenzothiophene desulfurization: purification and characterization from a non-desulfurizing bacterium, paenibacillus polymyxa a-1. | the dibenzothiophene (dbt) desulfurizing bacterium metabolizes dbt to form 2-hydroxybiphenyl without breaking the carbon skeleton. of the dbt desulfurization enzymes, dszc and dsza catalyze monooxygenation reactions, both requiring flavin reductase. we searched for non-dbt-desulfurizing microorganisms producing a flavin reductase that couples more efficiently with dszc than that produced by the dbt desulfurizing bacterium rhodococcus erythropolis d-1, and found paenibacillus polymyxa a-1 to be a ... | 2002 | 12226720 |
| isolation and characterization of a moderate thermophile, mycobacterium phlei gtis10, capable of dibenzothiophene desulfurization. | an organism, identified as mycobacterium phlei gtis10, was isolated based on its ability to use dibenzothiophene (dbt) as a sole source of sulfur for growth at 30-52 degrees c. similar to other biodesulfurization-competent organisms, m. phlei gtis10 converts dbt to 2-hydroxybiphenyl (2-hbp), as detected by hplc. the specific desulfurization activity of the 50 degrees c m. phlei gtis10 culture was determined to be 1.1+/-0.07 micromol 2-hbp min(-1) (g dry cell)(-1). m. phlei gtis10 can also utiliz ... | 2002 | 12226734 |
| application of multi-parameter flow cytometry using fluorescent probes to study substrate toxicity in the indene bioconversion. | the bioconversion of indene to cis-(1s,2r) indandiol, a potential key intermediate in the synthesis of merck's hiv protease inhibitor, crixivan trade mark, can be achieved using a rhodococcus strain. this study using rhodococcus i24 reports on the application of multiparameter flow cytometry for the measurement of cell physiological properties based on cytoplasmic membrane (cm) integrity and membrane depolarization as indicators of toxic effects of the substrate, indene. quantification of intact ... | 2002 | 12226855 |
| identification of the source of faecal pollution in contaminated rivers. | identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. four independent assays for faecal source discrimination have been developed and implemented in our lab. these assays detect fluorescent whitening agents, faecal sterols, bifidobacterium adolescentis and rhodococcus coprophilus. the combination of these indicators is able to identify human derived faecal pollution in rivers containing inputs from septic tanks, municipal oxidatio ... | 2002 | 12227609 |
| central venous catheter-related bacteremia due to tsukamurella species in the immunocompromised host: a case series and review of the literature. | we report 6 cases of bacteremia due to tsukamurella species, all of which were in immunosuppressed patients with indwelling central venous catheters (cvcs). fewer than 20 cases of serious illness due to these gram-positive bacilli have been reported in the medical literature; these cases have mostly been ascribed to the species tsukamurella paurometabola. tsukamurella species are frequently misidentified as rhodococcus or corynebacterium species. we used high-performance liquid chromatography to ... | 2002 | 12228839 |
| [primary pyomiositis by rhodococcus spp. in an immunocompetent patient]. | 2002 | 12236992 | |
| molecular epidemiology of vapa-positive rhodococcus equi in thoroughbred horses in kagoshima, japan. | the prevalence of virulent r. equi having 15- to 17-kda antigens (vapa) in fecal isolates from 13 thoroughbred foals and their dams on 5 farms in kagoshima, japan, and the plasmid profiles of vapa-positive isolates by restriction fragment digestion patterns were investigated to compare the genotypic variation among virulence plasmids of r. equi isolates from japan. in total, 218 (24.6%) of 886 isolates from the feces of the 13 foals and 13 (12.5%) of 104 isolates from the feces of their dams dem ... | 2002 | 12237518 |
| rhodococcus equi pleuropneumonia in an adult horse. | a 10-year-old warmblood gelding was evaluated for intermittent pyrexia, dullness, weight loss, and progressive respiratory disease. multifocal necrotic pneumonia and pleuritis due to rhodococcus equi infection was diagnosed. case management is discussed, as well as factors that may have led to this rare cause of pleuropneumonia in an adult horse. | 2002 | 12240529 |
| bacterial flora in foals with upper respiratory tract infections in poland. | bacteria isolated from nasal cavity of 80 foals with upper respiratory tract infection, as well as from 20 healthy foals, were examined. within the group of sick animals, from 18 (22.5%) bacteria with recognized pathogenicity were isolated. coagulase-negative staphylococci and acinetobacter sp. were the dominant species identified (100 and 45%, respectively). no bacteria species with recognized pathogenicity were isolated from the group of healthy animals. three cases of death within the group o ... | 2002 | 12241031 |
| cholesterol oxidase and resistance of rhodococcus equi to peroxidative stress in vitro in the presence of cholesterol. | rhodococcus equi is a well-characterized bacterial pathogen which lyses cell membranes with the help of cholesterol oxidase (co). survival in macrophages is warranted by its ability to resist reactive radicals via catalase and superoxide dismutase (sod). therefore, co production in the absence or presence of 0.1 % cholesterol and sensitivity to exogenous hydrogen peroxide (h2o2) and superoxide anion (soa) were tested in seven strains of r. equi in vitro. when r. equi strains were grown on agar p ... | 2002 | 12241035 |
| evaluation of 5 serologic assays to detect rhodococcus equi pneumonia in foals. | to determine the sensitivity and specificity of 5 serologic assays used to diagnose rhodococcus equi pneumonia in foals and to determine whether any of the assays could be used to identify affected foals prior to the onset of clinical signs or to differentiate between affected and unaffected foals when clinical signs first become apparent. | 2002 | 12322921 |
| cloning, sequencing, and characterization of the hexahydro-1,3,5-trinitro-1,3,5-triazine degradation gene cluster from rhodococcus rhodochrous. | hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a high explosive which presents an environmental hazard as a major land and groundwater contaminant. rhodococcus rhodochrous strain 11y was isolated from explosive contaminated land and is capable of degrading rdx when provided as the sole source of nitrogen for growth. products of rdx degradation in resting-cell incubations were analyzed and found to include nitrite, formaldehyde, and formate. no ammonium was excreted into the medium, and no dead ... | 2002 | 12324318 |
| molecular and functional characterization of the kstd2 gene of rhodococcus erythropolis sq1 encoding a second 3-ketosteroid delta(1)-dehydrogenase isoenzyme. | previously, rhodococcus erythropolis sq1 kstd, encoding ketosteroid delta(1)-dehydrogenase (kstd1) was characterized. surprisingly, a kstd gene deletion mutant (strain rg1) grew normally on steroids. uv mutagenesis of strain rg1 allowed isolation of strains (e.g. strain rg1-uv29) unable to perform the delta(1)-dehydrogenation of 4-androstene-3,17-dione (ad) and 9alpha-hydroxy-4-androstene-3,17-dione (9ohad). functional complementation of strain rg1-uv29 with total genomic dna of strain rg1 resul ... | 2002 | 12368462 |
| biochemical characterization and structural analysis of a highly proficient cocaine esterase. | the bacterial cocaine esterase, coce, hydrolyzes cocaine faster than any other reported cocaine esterase. hydrolysis of the cocaine benzoyl ester follows michaelis-menten kinetics with k(cat) = 7.8 s(-1) and k(m) = 640 nm. a similar rate is observed for hydrolysis of cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. the high catalytic proficiency, lack of observable product inhibition, and ability to hydrolyze both cocai ... | 2002 | 12369817 |
| the cbs mutant strain of rhodococcus erythropolis ka2-5-1 expresses high levels of dsz enzymes in the presence of sulfate. | two mutants of the dibenzothiophene-desulfurizing rhodococcus erythropolis ka2-5-1, strains ms51 and ms316, which express a high level of desulfurizing activity in the presence of sulfate, were isolated using the transposome technique. the level of dibenzothiophene-desulfurization by cell-free extracts prepared from mutants ms51 and ms316 grown on sulfate was about five-fold higher than that by cell-free extracts of the wild-type. this result was consistent with results of western-blot analysis ... | 2002 | 12375103 |
| selective transport and accumulation of alkanes by rhodococcus erythropolis s+14he. | selective transport and accumulation of n-alkanes by rhodococcus erythropolis s+14he was studied by growing cells on n-hexadecane, n-octadecane or the branched alkane pristane, and on mixtures of hydrocarbons. ultrastructural analysis by transmission electron microscopy (tem) revealed hydrocarbon inclusion bodies present in cells grown on the three alkanes, but not in cells grown on soluble media or exposed to nonmetabolized 2,2,4,4,6,8,8-heptamethylnonane (hmn). n-hexadecane had the highest rat ... | 2002 | 12378606 |
| purification and characterization of the monooxygenase catalyzing sulfur-atom specific oxidation of dibenzothiophene and benzothiophene from the thermophilic bacterium paenibacillus sp. strain a11-2. | a benzothiophene (bt) and dibenzothiophene (dbt) monooxygenase (tdsc), which catalyzes the oxidation of the sulfur atoms in bt and dbt molecules, was purified from paenibacillus sp. strain a11-2. the molecular mass of the purified enzyme and its subunit were determined to be 200 kda and 43 kda by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, indicating a tetrameric structure. the n-terminal amino acid sequence of the purified tdsc completely matched ... | 2002 | 12382053 |
| [effect of shale kerogen oxidation products on biodegradation of petroleum and petroleum products in soil and water]. | effect of oxidation products of shale kerogen (high-molecular-weight acids with 6-22 carbon atoms) on biodegradation of oil and oil products in soil and water was studied. high-molecular-weight acids (hmwa) not only affected the layer of oil and/or oil products and dispersed it into small particles, but also stimulated growth of rhodococcus erythropolis vkm as-1339d, degraders of oil and oil products. addition of 0.001-0.003% hmwa to a medium to be purified from oil products increased the extent ... | 2002 | 12391752 |
| [biosensor of the reactor type based on rhodococcus erythropolis hl tm-1 cells for determining 2,4-dinitrophenol]. | a model of a reactor-type biosensor based on the rhodococcus erythropolis hl pm-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-dnp). the effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. in case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-dnp detection was 20 microm and the time of single ... | 2002 | 12391763 |
| sulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated rhodococcus sp. strains. | new desulfurizing bacteria able to convert dibenzothiophene into 2-hydroxybiphenyl and sulfate were isolated from contaminated soils collected in mexican refineries. random amplified polymorphic dna analysis showed they were different from previously reported rhodococcus erythropolis desulfurizing strains. according to 16s rrna gene sequencing and fatty acid analyses, these new isolates belonged to the genus rhodococcus. these strains could desulfurize 4,6-dimethyldibenzothiophene which is one o ... | 2002 | 12393216 |
| cerebral infection with rhodococcus equi in a heart transplant recipient. | iatrogenic immunosuppression following organ transplantation leads to an increased number of opportunistic bacterial and viral infections. similar to patients with human immunodeficiency virus (hiv) infection transplant recipients may suffer from rare infectious diseases. herein we report on a patient presenting with a cerebral infection who had previously undergone orthotopic cardiac transplantation. rhodococcus equi was isolated. | 2002 | 12398884 |
| gas exchange during intense exercise in standardbreds with earlier rhodococcus equi pneumonia. | it is not known if pulmonary function and gas exchange during exercise are altered after pyogranulomatous pneumonia caused by rhodococcus equi infection in the foal. the aim was to evaluate whether pulmonary gas exchange during high intensity exercise was altered in mature standardbreds with a history of r. equi pneumonia as foals. in 7 foals, r. equi pneumonia was confirmed and treated. at age 3 years, when these horses were subjected to professional training, an inclined treadmill exercise tes ... | 2002 | 12405730 |
| pac1 and pacap expression, signaling, and effect on the growth of hct8, human colonic tumor cells. | the pituitary adenylate cyclase-activating polypeptide (pacap) type 1 receptor (pac1) is a heptahelical, g protein-coupled receptor that has been shown to be expressed by non-squamous lung cancer and breast cancer cell lines, and to be coupled to the growth of these tumors. we have previously shown that pacap and its receptor, pac1, are expressed in rat colonic tissue. in this study, we used polyclonal antibodies directed against the cooh terminal of pac1, as well as fluorescently labeled pacap, ... | 2002 | 12409223 |
| enantioselective stereoinversion in the kinetic resolution of rac-sec-alkyl sulfate esters by hydrolysis with an alkylsulfatase from rhodococcus ruber dsm 44541 furnishes homochiral products. | 2002 | 12412078 | |
| identification of pulmonary t-lymphocyte and serum antibody isotype responses associated with protection against rhodococcus equi. | rhodococcus equi infects and causes pneumonia in foals between 2 and 4 months of age but does not induce disease in immunocompetent adults, which are immune and remain clinically normal upon challenge. understanding the protective response against r. equi in adult horses is important in the development of vaccine strategies, since those mechanisms likely reflect the protective phenotype that an effective vaccine would generate in the foal. twelve adult horses were challenged with virulent r. equ ... | 2002 | 12414760 |
| versatile persistence pathways for pathogens of animals and plants. | the glyoxylate cycle and the glycine cleavage system are part of conserved metabolic pathways involved in the chronic persistence of microorganisms in animal hosts. in the chromosome of the plant pathogen rhodococcus fascians, the vic locus has been identified as a region containing genes essential for persistence inside induced leafy galls. sequence analysis showed that this 18-kb locus is syntenic with chromosomal regions of mycobacterium species that encompass the 'persistence' loci of these ... | 2002 | 12419605 |
| a novel class of self-sufficient cytochrome p450 monooxygenases in prokaryotes. | the bacillus cytochrome p450 bm3 integrates an entire p450 system in one polypeptide and represents a convenient prokaryotic model for microsomal p450s. this self-sufficient class ii p450 is also present in actinomycetes and fungi. by genome analysis we have identified additional homologues in the pathogenic species bacillus anthracis and bacillus cereus, and in ralstonia metallidurans. this analysis also revealed a novel class of putative self-sufficient p450s, p450 pfor, comprising a class i p ... | 2002 | 12419614 |