Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| detection of externally induced impairments in single bacterial cells by laser microbe mass analysis. | applying the laser microbe mass analyzer method (lamma), mass spectra (fingerprints) were taken from single bacterial cells not treated or treated with high temperature, x-irradiation, isonicotinic acid hydrazide (inh), or diaminodiphenylsulfone (dds). spectra of treated cells ("m. lufu," m. tuberculosis h37ra, e. coli) differ from those of controls in that the k+/na+ ratio was smaller and in that the intensities of peaks with m/e greater than 100 were lower. from the results with m. leprae the ... | 1982 | 6804403 |
| increase in prevalence of leprosy caused by dapsone-resistant mycobacterium leprae. | 1982 | 6798411 | |
| dna isolated from mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical dna-dna reassociation. | dna derived from mycobacterium leprae (grown in armadillos) was isolated, purified, and analyzed spectrophotometrically. the genome size and the guanine-plus-cytosine content of m. leprae were 1.3 x 10(9) and 55.8%, respectively. among selected strains of mycobacterial, nocardial, and corynebacterial species, corynebacterium sp. 2628 lb, isolated from a human leprosy patient, showed the highest dna homology with m. leprae. of the dnas derived from mycobacteria, those of m. tuberculosis and m. sc ... | 1982 | 6801025 |
| applications of flow cytometry on bacteria: cell cycle kinetics, drug effects, and quantitation of antibody binding. | 1982 | 7035105 | |
| thymus-dependent lymphocytes in leprosy. ii. effect of chemotherapy on t-lymphocyte subpopulations. | the basis of the immunological unresponsiveness seen in leprosy patients is unknown. untreated lepromatous leprosy patients display an unspecific cellular anergy which disappears with treatment, leaving an anergy specific for mycobacterium leprae. these patients suffer from a complication, erythema nodosum leprosum, characterized by a recurrent eruption of tender skin nodules disappearing in 2 to 3 days. these nodules show a histological picture reminiscent of an arthus reaction. erythema nodosu ... | 1982 | 7040440 |
| leprosy. | 1982 | 7040711 | |
| demonstration of mycobacterial antigens in leprosy tissues. | biopsies from 69 patients with leprosy were stained to demonstrate mycobacterial antigens using immunoperoxidase methods. the same biopsies were cut and stained using fite-faraco, triff and hematoxylin-eosin for classifying the patients and to demonstrate mycobacteria. since m. leprae and bcg show extensive antigenic cross reactions, anti-bcg antibodies were used as primary antisera to demonstrate cross-reacting antigens of m. leprae. cross-reacting mycobacterial antigens were, thus, found in al ... | 1982 | 7042609 |
| bacterial growth kinetics of "m. lufu" in the presence and absence of various drugs alone and in combination. a model for the development of combined chemotherapy against m. leprae? | bacterial growth kinetic studies were performed in a series of potential inhibitors of m. leprae using "m. lufu" as a model strain. reasons why "m. lufu" is considered to be a better model than m. tuberculosis are presented. the inhibitory power of the single drugs has been quantified, the activity constants are calculated, and the synergistic, additive, or antagonistic behavior of the combinations is evaluated. it is demonstrated that a combination consisting of dapsone (dds), prothionamide (pt ... | 1982 | 7042611 |
| ultrastructural features of the multiplication of human and murine leprosy bacilli in macrophages of nude mice. | ultrastructural features of the growth of m. leprae and m. lepraemurium in nude mouse macrophages were studied by ultrathin sectioning and freeze-etching. in nude mouse macrophages, m. leprae produced spherical droplets (foamy structures) similar to those in human lepra cells. on the other hand, m. lepraemurium produced typical crystalline material in nude mouse macrophages, which is quite the same as that observed in the c3h strain mouse, spherical droplets in the form of foamy structures seem ... | 1982 | 7042613 |
| ultrastructural characteristics of mycobacterium bovis bcg and mycobacterium leprae. | freeze-fracture studies (ff) and ultrathin sectioning (uts) have been performed on mycobacterium bovis (bcg) and mycobacterium leprae (ml). ff of bcg revealed the presence of two populations of cells: one group having four fracture faces and the other showing only two; the latter showed a preferential cleavage along a plane across the cell wall (cw) having a smooth appearance devoid of particles, on both protoplasmic (pf) and exoplasmic (ef) fracture faces. on the other hand, ff of the plasma me ... | 1982 | 7042614 |
| adenosine triphosphate content of mycobacterium leprae: effect of purification procedures. | various procedures to decontaminate and purify m leprae free of host tissue material resulted in total retention of their intracellular atp and also infectiousness. the atp content of one million m. leprae cells, isolated from either livers, spleens, or lymph nodes of infected armadillos, or a nude mouse foot pad or a human biopsy specimen, was in the range of 1.17 to 1.40 picograms. suspensions could be decontaminated with 4% naoh and all non-bacterial atp could be eliminated by the combined ac ... | 1982 | 7042615 |
| statistical analysis of results obtained by two methods for testing drug activity against mycobacterium leprae. | 1982 | 7042616 | |
| preliminary studies of the metabolic activity of purified suspensions of mycobacterium leprae. | mycobacterium leprae isolated from armadillo tissue incorporated radioactivity from d-(14c)glucose and (14c)protein hydrolysate. in the presence of glucose, the rate of incorporation of (14c)protein hydrolysate was increased. uptake of glucose was inhibited by 2-deoxy-d-glucose and sodium azide; that of the amino acids was inhibited by puromycin and chloramphenicol and, weakly, by cycloheximide. | 1982 | 7042905 |
| immunological implications of necrotic, cellular and vascular changes in leprous neuritis: light and electron microscopy. | 1982 | 7043135 | |
| rapid, radiolabeled macrophage culture method for detection of dapsone-resistant mycobacterium leprae. | mycobacterium leprae cells extracted from the skin biopsies of 14 bacilliferous lepromatous patients were maintained in human-murine macrophage cultures for 3 weeks in the presence of [3h]thymidine and dds (4,4'-diaminodiphenyl sulfone). all cultures except one containing freshly extracted viable bacilli showed significant incorporation of [3h]thymidine as compared to control cultures containing heat-killed bacilli of the corresponding strain. six susceptible strains of m. leprae obtained from u ... | 1982 | 7044299 |
| "pro-eu-karyote" graft acceptance: a mechanism for intracellular parasitism - a new hypothesis for pathogenesis of leprosy. | 1982 | 7045602 | |
| the origin, morphology, and function of epithelioid cells. | epithelioid cells are cells of the mononuclear phagocyte system found in certain granulomas mainly associated with intense immunological activity. these cells show little phagocytic activity. in certain experimental granulomas such as those produced in guinea pigs sensitive to zirconium, and at sites of intense inflammatory reaction in man, they may contain varying amounts of rough endoplasmic reticulum ("secretory" epithelioid cells). in other situations such as tuberculoid leprosy and in some ... | 1982 | 7047374 |
| humoral immunity in leprosy: immunoglobulin g and m antibody responses to mycobacterium leprae in relation to various disease patterns. | a solid-phase radioimmunoassay, applying whole mycobacterium leprae as antigen and radiolabeled protein a from staphylococcus aureus as antibody-detecting reagent, was used for the determination of specific immunoglobulin g (igg) and igm antibody responses in leprosy patients. high igg anti-m. leprae antibody levels were found in lepromatous leprosy patients, whereas the antibody response in tuberculoid leprosy patients varied from negative, i.e., comparable with responses measured in normal ind ... | 1982 | 7047400 |
| the clinical diagnosis of dapsone resistant leprosy. | for the early recognition of dapsone resistance, it is essential to regularly examine patients at risk for suspicious new lesions, backed by regular clinical treatment records including smear results. the earlier the recognition and starting on alternative treatment, the better is the response. however, it is not advisable to change treatment without attempting at least clinical confirmation, and endorsing the chart so that the problem is not missed on subsequent occasions. | 1982 | 7047897 |
| histologic profile in apparently normal skin of leprosy patients. | 1982 | 7047898 | |
| effect of desoxy fructo serotonin (dfs) on the multiplication of m. leprae in mouse foot-pad. | 1982 | 7047899 | |
| a comparison of the skin scrape and skin biopsy methods in mouse foot-pad experiments with m. leprae. | 1982 | 7047900 | |
| effects of propionibacterium acnes treatment on the course of mycobacterium leprae infection in mice. | studies were carried out to determine the effects of treatment with killed suspensions of propionibacterium acnes (formerly designated corynebacterium parvum) on the course of mycobacterium leprae infection in mice. systemic (intravenous or intraperitoneal) treatment with p. acnes failed to significantly alter the growth of m. leprae in the mouse footpad. in contrast, injections of p. acnes directly into the infected footpad markedly inhibited the growth of the leprosy bacilli regardless of whet ... | 1982 | 7049949 |
| n-acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase in mycobacterium leprae. | n-acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase activities were detected in cell-free extracts of mycobacterium leprae (from armadillo liver). extracts of bacteria which had been treated with 7-diazonaphthalene-1,3-disulphonic acid to inactivate surface enzymes retained 30-45% of the activity of the glycosidases and 15% of the activity of the acid phosphatase. when intact bacteria were treated with 1 m-naoh, the corresponding activity in the extracts was 4--9% for the glyc ... | 1982 | 7050296 |
| hansen's disease in the united states. | this article offers a general overview of hansen's disease (leprosy) and examines in some detail the spatial characteristics and demographic profiles of known sufferers. over the years the pejorative labels attached to the disease and the societal and psychological attitudes which produce and accompany them have stigmatized leprosy sufferers. unfortunately this stigma has often pre-empted rational understanding and treatment. contrary to popular belief, leprosy, caused by the bacteriological age ... | 1982 | 7051335 |
| reduction of potassium tellurite and atp content in mycobacterium leprae. | the purpose of this investigation was to examine those properties of mycobacterium leprae which might be useful in estimating the heterogeneity of the bacterial populations harvested from the tissues of experimentally infected armadillos. the following technical procedures were applied: fluorescent microscopy on smears stained by an auramine o-ethidium bromide dual procedure, fine structure observation of ultrathin sections, reduction of potassium tellurite as observed under the electron microsc ... | 1982 | 7051925 |
| metabolism of carbon sources by mycobacterium leprae: a preliminary report. | glucose was established in mycobacterium leprae by glycolysis and the hexose monophosphate pathway (30 %)-pentose phosphate pathway. glycerol was also catabolised to co2 at a similar rate to glucose. key in cell-free extracts as enzymes for m. leprae. | 1982 | 7051926 |
| fatty acid and polar lipid analysis as tools in the identification of mycobacterium leprae and some related slow-growing mycobacterial species. | some species of slow-growing mycobacteria, including mycobacterium leprae (1 strain), m. lepraemurium (2 strains), m. paratuberculosis (12 strains) and a group of 12 m. avium-like strains (isolates from wild animals) were examined by gas chromatography (gc) for cellular fatty acids and by thin-layer chromatography (tlc) for polar lipids. all the gc patterns, including that of m. leprae, contained high levels of tuberculostearic-, stearic-, octadecenoic- and palmitic acid. tetradecanoic-, pentade ... | 1982 | 7051927 |
| the mycolic acids of mycobacterium leprae harvested from experimentally infected nine-banded armadillos. | mycolic acid methyl esters were prepared from defatted cells of armadillo-derived mycobacterium leprae and analysed by thin-layer and high-performance liquid chromatography, proton magnetic resonance spectrometry and mass spectroscopy. the first type of mycolic acid characterized was an "alpha-mycolate" having two cis-cyclopropane rings, a 78-carbon main component and an overall size-range of 72 to 83 carbons. ketomycolates, with an 83-carbon main component, were the only other type of mycolate ... | 1982 | 7051928 |
| [comparative study of the lipids of mycobacterium leprae and other strains of mycobacteria]. | 1982 | 7051929 | |
| the who leprosy programme. | 1982 | 7051930 | |
| mycobacterium leprae and other mycobacteria difficult to culture. | 1982 | 7051931 | |
| mycobacterial lipids in infected tissue samples. | mycobacteria synthesize characteristic lipids which can readily be distinguished from those synthesized by the host cells which they infect during pathogenesis. this fact can be exploited to provide information about mycobacteria growing in infected tissue samples: (a) qualitative analysis of lipid extracts from lepromatous leprosy skin biopsies reveals the presence of several mycobacterial lipids including an m. leprae-specific glycolipid, phthiocerol dimycocerosate and mycolic acids; (b) quant ... | 1982 | 7051932 |
| ultrastructural alterations of mycobacterium leprae in skin biopsies of untreated and treated lepromatous patients. | 1. -- mycobacterium leprae cells under a process of progressive disaggregation are present in the skin of both treated and untreated patients. 2. -- the ultrastructural alterations observed during the degenerative process seem to be qualitatively similar in treated and untreated patients. 3. -- the proportion of altered m. leprae cells increases during the treatment, mainly with rifampicin and rifampicin + clofazimine + diaminodiphenyl sulfone. 4. -- the cell wall of m. leprae is the last bacter ... | 1982 | 7051933 |
| adsorption of mycobacteriophages on mycobacterium leprae: taxonomic significance. | the following bacteriophages were shown to adsorb on mycobacterium leprae: bk1, clark, sedge, baits, watson and d29. bacteriophages that did not adsorb on the leprosy bacilli were bg1, legendre, marshall, panetti, leo and wiseman. the taxonomic significance of these findings and some prospective consequences of these investigations are discussed. | 1982 | 7051934 |
| anomalous isoenzymes of lactate dehydrogenase in sera of patients with leprosy. | in an earlier study (saoji et al, 1980) we looked for anomalous (additional) isoenzymes in homogenates of 17 active and 25 of regressing lepromatous leprosy, three of erythema nodosum leprosum, eight of bt, 19 of tt and one of indeterminate leprosy. anomalous bands were found in 17 cases. they correlated with large numbers of viable organisms and were thought to originate from mycobacterium leprae. this communication describes the serum ldh isoenzymes of the same 78 cases. six of 17 cases with a ... | 1982 | 7098443 |
| bacteriological studies of armadillo livers infected with mycobacterium leprae. | 1982 | 7181543 | |
| reversion of the downhill course of active lepromatous leprosy by repeated transfusions of fresh blood, donated by healthy but lepromin positive patients. | earlier studies of immunologic reconstitution therapy of active lepromatous leprosy patients by repeated infusions of viable allogeneic blood lymphocytes indicated a beneficial effect. in order to avoid cell separation and its associated risks, we attempted to transfer immunity passively into seven seriously ill lepromatous patients by repeated transfusions of fresh blood donated by healthy, but tuberculin and lepromin positive, subjects. the results showed clinical improvement in some cases wit ... | 1982 | 7041343 |
| [pathogenic mycobacteria]. | it is well established that mycobacteria other than tubercle bacilli and m. leprae are important human pathogens. at least 15 species comprise all the presently known mycobacterial pathogens of man. eleven species are potential pathogens because disease production often dependent on special factors of the human host. the comments include some characterization of the mycobacteria, especially of the potential pathogenic species. a summary of important nontuberculous mycobacterial diseases of man i ... | 1982 | 7041441 |
| immunoglobulin class specific antibodies to m. leprae in leprosy patients, including the indeterminate group and healthy contacts as a step in the development of methods for sero-diagnosis of leprosy. | iga, igm and igg anti-m. leprae antibody activity was quantitated by solid phase radioimmunoassay in groups of untreated leprosy patients throughout the spectrum, in lepromatous leprosy patients treated for more than 10 years, in an indeterminate leprosy group, and in a non-leprosy control group. iga, igm and igg anti-m. leprae antibody activity increased from the group of healthy individuals exposed to m. leprae but without clinical signs of leprosy to tuberculoid (bt and bt/tt) and further to ... | 1982 | 7042137 |
| feline leprosy: a review of forty-four cases from western canada. | forty-four files of cats diagnosed as having feline leprosy were reviewed. all except one were from along the pacific coast in the province of british columbia. the majority of cats were between one and three years of age and there was an increase in the number of diagnosis during the winter months.two types of granulomatous responses similar to the tuberculoid and lepromatous phases of human leprosy were recognized. in spite of the overall similarities in the cellular response to human leprosy ... | 1982 | 17422188 |
| trials of culturing of mycobacterium leprae using media enriched with thyroid hormones. | 1983 | 6400163 | |
| amyloidosis and the serum amyloid a protein response to muramyl dipeptide analogs and different mycobacterial species. | serum amyloid a protein (saa) elevation accompanies induction of secondary amyloidosis in mice given mycobacterium butyricum in freund adjuvant. the synthesis of saa by cultured hepatocytes is induced by a macrophage-derived mediator, which has been identified as interleukin 1. in these studies, saa synthesis has been used as an index of macrophage activation to examine the in vivo response of mice to challenge with seven different mycobacteria and with synthetic analogs of the immunoadjuvant n- ... | 1983 | 6601620 |
| t-cell conditioned media reverse t-cell unresponsiveness in lepromatous leprosy. | in some subjects the infective agent of leprosy, mycobacterium leprae, causes disseminated (lepromatous) disease. such subjects have a major role in the transmission of the disease and show deficient t-cell responses both in vivo and in vitro to m. leprae, but not to other antigens. numerous studies have recently shown that t cells with functional capabilities after initial triggering with antigen can be maintained in a state of continuous proliferation in vitro when cultured in medium containin ... | 1983 | 6602296 |
| lymphocyte subpopulations in mice infected with mycobacterium leprae. | t and b cells were quantitated from the spleen of m. leprae infected mice and correlated with bacillary count in the foot pad. lymphocyte transformation with pha and m. leprae (armadillo) antigens was also studied during different months of infection. t cell counts dropped gradually but significantly throughout the course of infection. b cells had a concomitant rise up to 6 months and then registered a fall as compared to the initial control figures. transformation of lymphocytes with pha fell s ... | 1983 | 6603563 |
| monocyte-derived soluble suppressor factor(s) in patients with lepromatous leprosy. | peripheral blood monocytes from polar lepromatous leprosy (ll) patients were unable to support mycobacterium leprae-induced in vitro lymphoproliferation of hla-d-matched t cells from tuberculoid leprosy subjects, whereas those from responder individuals were able to do so. monocyte-rich adherent cells from untreated ll patients released de novo soluble factors which inhibited antigen-induced lymphoproliferation to a greater extent and mitogenic responses to a lesser extent. suppressive activity ... | 1983 | 6605932 |
| leprosy in sub-human primates: potential risk for transfer of mycobacterium leprae to humans. | 1983 | 6668987 | |
| in vitro cultivation of mycobacterium x from mycobacterium leprae-infected tissues in acetone-dimethylsulfoxide-tetradecane medium. | several strains of mycobacteria were cultivable from mycobacterium leprae-infected human and armadillo tissues in a liquid medium containing three dimethyl analogs: dimethylketone, dimethylsulfoxide, and tetradecane [ch3 . (ch2)12 . ch3]. the medium contained kh2po4, 7.0 g; na2hpo4, 1.0 g; (nh4)2so4, 2.0 g; mgso4, 0.1 g; iron ammonium citrate, 0.1 g; dmso, 10 ml; and acetone, 150 ml in distilled water ad one liter. tetradecane 0.1 ml was added aseptically to each tube, containing 10 ml of the st ... | 1983 | 6683261 |
| ige in leprosy; effect of a mycobacterium leprae-bcg vaccine. | since some previous studies have reported elevated total serum ige levels in leprosy, that may be associated with the existence of a state of generalized t cell anergy, we undertook a carefully controlled study of this immunoglobulin in such patients before and after treatment with a mycobacterium leprae-bcg vaccine. we found, firstly, that lepromatous leprosy patients suffering active disease had only a moderate elevation of ige levels that was not statistically significant when compared to app ... | 1983 | 6684643 |
| survival of mycobacterium leprae in mice administered several antibiotics per os. | 1983 | 6684648 | |
| foam--the result of an interaction between unactivated macrophages and dead mycobacterium leprae? | 1983 | 6684649 | |
| infection of the congenitally athymic rat with mycobacterium leprae. | the susceptibility of congenitally athymic rats to mycobacterium leprae infection has been investigated. following inoculation of small numbers of m. leprae (5 x 10(3] into the foot pad, the organisms replicated and attained a maximum of 2.6 x 10(8) per foot pad at 294 days; there was limited dissemination to the tail. in similarly inoculated neonatally thymectomized lewis rats (ntlrs) a ceiling of 2 x 10(7) organisms was reached. when a larger inoculum (10(7] was given, the number of bacilli in ... | 1983 | 6685695 |
| variable lepromin response to mycobacterium leprae in resistant armadillos. | eight armadillos resistant to the infection of mycobacterium leprae were lepromin tested. the tissue response was tuberculoid in 5, borderline in 2, and lepromatous in 1, thus showing a wide variation. it is seen that although cell-mediated immunity as evidenced by a tuberculoid granulomatous response to killed m. leprae is associated with resistance to the disease, there may be other yet unknown factors which protect armadillos from the infection. lepromin responses were recognized histological ... | 1983 | 6685696 |
| dds-resistant infection among leprosy patients in the population of gudiyatham taluk, south india. part 3. prevalence, incidence, risk factors, and interpretation of mouse foot pad test results. | at the schieffelin leprosy research and training centre, karigiri, india, a study of the population of gudiyatham taluk revealed that the prevalence of dapsone (dds)-resistant infection among lepromatous (ll) and borderline lepromatous (bl) leprosy patients treated for a minimum of three years was 3.3% (33 per 1000), with an average annual incidence of 0.28% per year. dds-resistant infection was diagnosed when review of skin smear readings showed a continuing increase in the number of mycobacter ... | 1983 | 6685699 |
| the significance of dapsone (dds)-resistant mycobacterium leprae in untreated patients. | in a stable rural population of south india, 18 consecutive untreated persons newly discovered to have leprosy with a bacterial index (bi) greater than or equal to 2+ were tested for mycobacterium leprae resistant to dapsone (dds) by the mouse foot pad test. of 12 successful tests, five detected resistant m. leprae. known contact with a treated patient in the ten years preceding the diagnosis of leprosy was not found to increase the risk of dds-resistant m. leprae occurring in an untreated, newl ... | 1983 | 6685700 |
| response to dapsone (dds) monotherapy in leprosy patients of gudiyatham taluk, south india: comparison between the 1960s and the 1970s. | at the schieffelin leprosy research and training centre, karagiri, india, 148 lepromatous (ll) and borderline lepromatous (bl) leprosy patients registered for treatment in the years 1971 to 1973 were found to respond as well to dapsone (dds) monotherapy as 391 ll and bl patients registered in 1964 to 1966, as indicated by clearance of mycobacterium leprae from skin smears during the initial seven years of therapy in each patient. apparently, the efficacy of dds monotherapy has not been progressi ... | 1983 | 6685701 |
| [change in cell-mediated immunity to mycobacterium leprae and mycobacterium marinum in hansen's disease patients]. | the cell mediated immunity (cmi) to protein purified derivates of mycobacterium leprae, mycobacterium tuberculosis, mycobacterium avium and mycobacterium marinum was studied. leukocyte migration inhibition (lmi) and delayed hypersensitivity skin reactions to these antigens were examined in 44 hanseniasis patients (20 quiescent virchowians, 13 reactional virchowians and 11 tuberculoid patients) and 15 healthy subjects. an impairment in lmi and delayed hypersensitivity tests to m. leprae and m. ma ... | 1983 | 6432692 |
| [immunosuppression mechanisms in leprosy]. | 1983 | 6371439 | |
| taxonomy of mycobacterial strains isolated from the tissues of leprosy patients. | thirty-six slowly growing mycobacteria isolated from the tissues of leprosy patients were studied using 40 characteristics as well as susceptibility to 27 distinct mycobacteriophages. the composition in mycolic acids of selected strains was also studied. according to the data, the strains formed 5 clusters. some of the clusters were possibly as yet undescribed species; however, comparison of the data with the known properties of mycobacterium leprae leads to the conclusion that none of the strai ... | 1983 | 6372573 |
| [new trends in the treatment of leprosy]. | 1983 | 6374636 | |
| [structure of the lipid constituents of mycobacterium leprae. comparison with various species of mycobacteria]. | 1983 | 6377796 | |
| [action of desoxyfructo-serotonin on mycobacterium leprae. results of inoculation of human biopsy specimens in the mouse after a year of treatment]. | m. leprae, extracted from cutaneous biopsies of 4 out of 7 patients treated with dfs during one year at doses of 1000 to 1500 mg/day were injected in mouse foot pads. bacterial multiplication was observed in each case. the purely bacteriostatic activity of dfs for m. leprae, previously observed in mice, is thus confirmed. the eventual place of dfs in the therapy of leprosy is discussed. | 1983 | 6377799 |
| [indirect immunofluorescence for detecting antibodies against m. leprae. application to a group of patients and persons living with them]. | 1983 | 6379769 | |
| [the mitsuda reaction and its antigen, good subjects for a bibliography]. | some references of the history of mitsuda reaction and its antigen, as well as for the results and significance of this unique reaction are presented. in spite of the great advances of modern immunology. mitsuda reaction is still the best method for the diagnosis of the forms of hanseniasis and for the prognosis of the disease in patients and in healthy persons. | 1983 | 6381300 |
| [in vitro behavior of macrophages of healthy subjects and hansen patients against mycobacterium leprae]. | the in vitro phagocytic capacity of human macrophages facing m. leprae was analysed in a selected sample of healthy and hanseniasis patients (virchowian and tuberculoid) individuals. families where at least one of the spouses presented hansen's disease were selected and 176 white people were studied. the analysis of the macrophages' behavior was performed by reading the slides in the 5th, 10th, 15th, 20th and 30th days after inoculation of m. leprae. the classification is discussed. the results ... | 1983 | 6381301 |
| [familial analysis of the in vitro behavior of human macrophages against mycobacterium leprae]. | the lysogenic capacity of human macrophages facing m. leprae in vitro may be dependent on an important genetic component. although the family aggregation of the trait is demonstrated, this is a necessary but not sufficient condition to prove genetic influence. the data do not fit some simple genetic models (autosomal dominant or incompletely dominant gene; dominant or recessive sex-linked gene). the results obtained are consistent with the hypothesis that the macrophages' lysogenic capacity is m ... | 1983 | 6381302 |
| mouse foot pad technique for evaluation of drug resistance of m. leprae and other laboratory techniques to be used in leprosy control programmes. | there is no need for mouse foot pad facilities for drug sensitivity testing to start leprosy control programmes, since the size of the problem is known. there will however be a need for some mouse foot pad work in later stages, to document relapse cases and test relapse strains for drug sensitivity. arrangements should be made to ship such specimens to existing laboratories. there is more need however for facilities for monitoring drug intake. finally, where nonexistant, there may be incidentall ... | 1983 | 6401137 |
| the bacteriology of mycobacterium leprae. | 1983 | 6405519 | |
| serological activity of a characteristic phenolic glycolipid from mycobacterium leprae in sera from patients with leprosy and tuberculosis. | serological activity against a purified phenolic glycolipid from mycobacterium leprae, which may be obtained in large amounts from m. leprae infected armadillo liver, was investigated using immunodiffusion and an enzyme linked immunosorbent assay (elisa). generally a good correlation was obtained between these techniques, but the elisa was more sensitive and convenient. relatively high igg and igm anti-glycolipid antibody levels were found in lepromatous leprosy patients. the antibody titres to ... | 1983 | 6407793 |
| isolation of a characteristic phthiocerol dimycocerosate from mycobacterium leprae. | a characteristic mycobacterial wax, phthiocerol dimycocerosate, has been isolated from liver of armadillos experimentally infected with mycobacterium leprae. the structure of this wax is generally similar to that produced by mycobacterium tuberculosis, but the homologous phthiocerol and the mycocerosic acid components from m. leprae are significantly different from those of m. tuberculosis. | 1983 | 6409988 |
| demonstration of mycobacterial antigens in nerve biopsies from leprosy patients using peroxidase-antiperoxidase immunoenzyme technique. | peripheral nerve biopsies from patients with leprosy were stained with anti-mycobacterium bovis (bcg) in a peroxidase-antiperoxidase (pap) system to demonstrate intraneural mycobacterial antigens. most m. leprae antigens have been shown to cross-react with bcg. of the 30 biopsies from borderline tuberculoid (bt) patients 18 had acid-fast bacilli while 26 of them had demonstrable mycobacterial antigens in their nerves. all borderline lepromatous (bl) and lepromatous leprosy (ll) nerve biopsies ha ... | 1983 | 6416726 |
| defective gamma interferon production in leprosy. reversal with antigen and interleukin 2. | antigen and mitogen-induced gamma interferon (gamma-ifn) production was studied in peripheral blood mononuclear cells from 34 leprosy patients. 17 of 18 lepromatous leprosy and borderline lepromatous patients (ll and bl) failed to release gamma-ifn in response to specific antigen (mycobacterium leprae) and displayed reduced responses to mitogen (concanavalin a) stimulation. in contrast, cells from six tuberculoid and borderline tuberculoid patients (tt and bt) produced considerable levels of gam ... | 1983 | 6417263 |
| utilization of indole compounds by cryptococcus neoformans to produce a melanin-like pigment. | several indoles served as substrates for the phenoloxidase of cryptococcus neoformans and resulted in the production of a melanin-like pigment. in general, a higher percentage of c. neoformans var. neoformans (a and d serotypes) isolates could produce pigment from indoles than could those of var. gattii (b and c serotypes). only compounds with a hydroxyl or an amino group on the phenyl ring produced pigment; methoxy, nitro, methyl, and fluorine substituents on the phenyl ring were inactive, as w ... | 1983 | 6418759 |
| fernandez and mitsuda reactivity in guinea pigs sensitized with heat-killed mycobacterium leprae: persistence and specificity of skin reactivity to soluble and particulate antigens. | guinea pigs were sensitized with 500 micrograms dry weight of heat-killed mycobacterium leprae, m. tuberculosis h37rv, m. vaccae, or m. nonchromogenicum suspended in saline. significant fernandez (peak swelling at 48 hr) and mitsuda (peaking at 21 days) reactions were observed when all four groups of animals were skin tested with 10 micrograms of the homologous whole-cell antigen (wca) preparations one month after sensitization. some of the guinea pigs were given a booster injection of the homol ... | 1983 | 6423556 |
| ecological considerations on treatment of leprosy. | 1983 | 6381451 | |
| [growth inhibition of mycobacterium lepraemurium in a cell-free medium by adding a suspension of m. leprae]. | 1983 | 6381452 | |
| [susceptibility of rhino mouse to mycobacterium leprae]. | 1983 | 6381457 | |
| mechanism of immunosuppression in leprosy: presence of suppressor factor(s) from macrophages of lepromatous patients. | human peripheral blood mononuclear cell proliferation induced by mycobacterium leprae could be inhibited by the suppressor factor in the lysate of the macrophages of lepromatous leprosy patients. macrophages from normal subjects and tuberculoid patients did not show production of a suppressor factor. inhibition occurred only when the factor was present in the initial stages of lymphocyte culture. the factor is heat stable and nondialyzable. proliferation induced by some mycobacteria and concanav ... | 1983 | 6221997 |
| immunology of human leprosy--current status. | 1983 | 6224992 | |
| reactions in leprosy. | 1983 | 6224993 | |
| mechanisms of t-cell unresponsiveness in leprosy. | we analysed the mechanisms of t-cell unresponsiveness to mycobacterium leprae antigens and to unrelated antigens or t-cell mitogens in human leprosy and in an experimental model of murine infection by m. lepraemurium (mlm). in human leprosy, monoclonal antibodies okt3, okt4 and okt8 were used to enumerate t-cell subpopulations within peripheral blood. increased percentages of okt8+ cytotoxic/suppressor cells were observed in untreated, non-reactional lepromatous patients. conversely, lepromatous ... | 1983 | 6226239 |
| early events in the host-parasite relationship and immune response in clinical leprosy: its possible importance for leprosy control. | 1983 | 6228348 | |
| suppressor cell activity and phenotypes in the blood or tissues of patients with leprosy. | suppressor cell activity has been demonstrated in the peripheral blood of patients with leprosy. cells bearing the suppressor/cytotoxic phenotype have been enumerated in both peripheral blood and tissues, and microanatomical differences in tissue distribution have been observed. this first generation of studies has been characterized by considerable disagreement, a not unusual circumstance in the study of leprosy. in the case of blood suppressor cell activity, there appears to be no doubt as to ... | 1983 | 6228349 |
| immunological aspects of human leprosy. | 1983 | 6230487 | |
| the growth of mycobacterium leprae in nude mice. | 1983 | 6230496 | |
| exochelin-mediated iron uptake into mycobacterium leprae. | iron chelated to the exochelins from mycobacterium neoaurum was taken up by a suspension of m. leprae, prepared from the liver of an infected armadillo, over 15 hr. no uptake occurred when the iron was chelated with exochelins from m. bovis bcg or m. smegmatis or to a single exochelin from m. vaccae. uptake appeared to be by facilitated diffusion since it was not inhibited by either hgcl2, nan3, or 2,4-dinitrophenol. this was similar to the mode of uptake of ferriexochelin into m. neoaurum itsel ... | 1983 | 6231257 |
| investigations related to the development of a leprosy vaccine. | 1983 | 6231259 | |
| comparison of the cytoplasmic antigens of leprosy-derived corynebacteria and some mycobacteria. | the immunological relationship between leprosy-derived corynebacteria (ldc) and reference mycobacteria was analysed by crossed immunoelectrophoresis with intermediate gel. for this purpose, three reference systems (ldc15/anti-ldc15, ldc18/anti-ldc8, and ldc8/anti-ldc8) were developed. they showed 15 to 20 distinct antigenic components in ldc cytoplasm. extensive cross-reactivity was observed among different ldc isolates, affecting 3 to 17 components. moreover, several components were shown to cr ... | 1983 | 6191381 |
| definition of species specific and cross-reactive antigenic determinants of mycobacterium leprae using monoclonal antibodies. | four soluble antigens of mycobacterium leprae have been identified using 12 murine monoclonal antibodies. their specificity, taxonomic distribution and molecular nature were analysed by radioimmunoassays and by immunoblotting from polyacrylamide electrophoresis gels. a protein antigen my1 (12k) reacted with one antibody (ml06) without demonstrable cross-reactivity for any of the other 20 tested species of mycobacteria. another four antibodies which identified antigen my2 revealed only a marginal ... | 1983 | 6191891 |
| serological specificity of phenolic glycolipid i from mycobacterium leprae and use in serodiagnosis of leprosy. | the serological activities of the specific phenolic glycolipid i from mycobacterium leprae, its dissected parts, and related glycolipids from other mycobacteria were examined by enzyme-linked immunosorbent assay against hyperimmune anti-m. leprae rabbit antiserum and sera from patients with leprosy and other mycobacterial diseases. high anti-phenolic glycolipid i immunoglobulin m antibodies were found in 23 of 24 (96%) of lepromatous leprosy patients on short term chemotherapy and in 8 of 13 tub ... | 1983 | 6193065 |
| bacteriology of mycobacterium leprae. | 1983 | 6193386 | |
| the degradation of mycobacterium leprae by a comparison of its staining properties. | the disintegration of mycobacterium leprae is revealed by a study of its acid-fast component, its non-acid-fast cell walls using methenamine silver, and its bcg-positive cytoplasmic component. solid bacilli stain by the three stain techniques used to identify these products, but the bcg component is demonstrated only with difficulty and appears granular. degradation of m. leprae is fairly rapid in bt, bb, and bl, and clearance of bacillary products occurs almost simultaneously because of the des ... | 1983 | 6194126 |
| characterization and functional studies of the murine t-lymphocyte response to mycobacterium leprae antigen. | mice were immunized with mycobacterium leprae in incomplete freund's adjuvant, and sensitized lymphocytes were obtained from draining lymph nodes. the lymphocytes thus obtained proliferated specifically in vitro in the presence of m. leprae antigen, and this response was shown to be both t-cell and macrophage dependent. t-cell blasts generated in vitro in response to m. leprae antigen were grown in the presence of interleukin-2 (il-2). the proliferative response of these blasts to m. leprae anti ... | 1983 | 6194557 |
| effect of pyridine extraction on the acid fastness of mycobacteria. | the effect of pyridine extraction on mycobacterial acid-fastness has been studied using m. leprae and some cultivable mycobacteria. the results revealed that not only m. leprae but some other mycobacteria, notably, m. vaccae and m. phlei also lose their acid-fastness when extracted with fresh pyridine for 2 hours at room temperature. the implication of these findings is discussed. | 1983 | 6195453 |
| the chemotherapy of leprosy. | 1983 | 6195695 | |
| growth of mycobacterium leprae in a redox system. | mycobacteria recovered from human lepromatous nodules and presumably m. leprae, have been grown in a medium that ensured a minimal oxygen tension at initiation of growth, and an increasing availability of oxygen as bacillary growth increased requiring marginal increments in oxygen tension. this physicochemical environment was achieved by the addition of strong biological reductants in the medium, and a combination of partial vacuum and alkaline pyrogallol in the culture vessel. in addition, n-te ... | 1983 | 6197589 |
| [stain behavior of mycobacterium leprae. a historical review]. | 1983 | 6197746 | |
| serodiagnosis of infection with mycobacterium leprae. | 1983 | 6200450 | |
| a serological test for leprosy based on competitive inhibition of monoclonal antibody binding to the my2a determinant of mycobacterium leprae. | a novel serological assay for leprosy has been devised on the basis of serum inhibition of binding of 125i-labelled ml04 monoclonal antibody to mycobacterium leprae sonicate-coated microtitre plates. antibodies were detected in 93% of lepromatous leprosy patients, whereas controls from the endemic area, including leprosy contacts and patients with tuberculosis, were serologically negative. the specificity and efficacy of the test may offer an advantage over previously used techniques. | 1983 | 6198787 |
| suppression of the antibody response to sulfolipids in leprosy patients by 4,4'-diaminodiphenyl sulfone (dds). | antibodies to sulfolipids were demonstrated in patients suffering from lepromatous leprosy. the antibody titre was found to decrease gradually on treatment with dds. this effect was maximum for patients undergoing treatment for more than 1 year. | 1983 | 6370132 |