Publications

[composition of normal bacterial flora of human skin in relation to the age and sex of examined persons].		1984	6536032
corynebacterium parvum toxicity in patients with limited and advanced malignancy.	the toxicity of intravenously administered corynebacterium parvum was observed in 14 patients with stage ii melanoma and in 14 patients with advanced ovarian carcinoma. those with melanoma were rendered disease-free by surgery prior to treatment. the ovarian cancer patients had failed chemotherapy with alkylating agents and were receiving c. parvum prior to chemotherapy as part of an immunochemotherapy trial. both clinical and laboratory parameters were observed. the mean daily c. parvum dose fo ...	1984	6539697
activation of peritoneal lymphocyte cytotoxicity in patients with ovarian cancer by intraperitoneal treatment with corynebacterium parvum.	following an intensive course of combination chemotherapy, patients with minimal residual ovarian cancer were treated with increasing concentrations of intraperitoneal corynebacterium parvum to assess whether or not natural killer (nk) cells could be activated. immunotherapy was administered every 2 weeks, initially with a dose of 250 micrograms/m2, which was progressively escalated as tolerated. each treatment induced a peritoneal cellular exudate which consisted primarily of neutrophils 48h af ...	1984	6541243
the protective effect of pretreatment with killed corynebacterium parvum against acute babesiosis in calves.	yearling calves were pre-treated intravenously (iv) with a 0.20 mg kg-1 dose of corynebacterium parvum and challenged after 30 days by iv inoculation of 3 x 10(9) babesia bigemina. the relatively low 0.20 mg kg-1 dose of c. parvum enhanced resistance as indicated by lower mean babesia parasitemias and less severe decreases in packed-cell volumes than in non-treated calves, but failed to stimulate a significant (p less than or equal to 0.05) level of protection against b. bigemina challenge.	1984	6541833
induction of chemiluminescence during interaction of tumoricidal effector cell populations and tumor cells is dependent on the presence of mycoplasma.	a variety of host cells, such as activated macrophages, natural killer (nk) cells, and polymorphonuclear leukocytes (pmnl), are cytotoxic for an array of non-antibody-coated tumor cells. because such effector cells appear to use oxygen-dependent mechanisms to effect tumor cell destruction in certain systems, the possibility of an involvement of toxic oxygen species has been considered. to investigate whether interaction of effector cells with neoplastic cells induces the generation of reactive o ...	1984	6607953
comparison of antimetastatic effect against lewis lung carcinoma after intratumoral and intravenous injections of cell-wall skeleton of propionibacterium acnes c7 in c57bl/6 mice.	antimetastatic activity of cell-wall skeleton of propionibacterium acnes c7 (p. acnes-cws) in c57bl/6 mice varied depending on the injection route. the kinetics of antimetastatic effect against lewis lung carcinoma (3ll) revealed that the sooner intratumoral (it) injection of p. acnes-cws was carried out, the better the result. however, intravenous (iv) injection of p. acnes-cws produced the best result if p. acnes-cws was injected at about the time when metastases began to develop in the lungs. ...	1984	6609859
the enhancement of tumor cell susceptibility to macrophage binding and cytolysis by p-aminobenzoic acid-n-xyloside sodium salt (k-247).	the enhancement of tumor cell susceptibility to macrophage binding and cytolysis by the pretreatment of tumor cells by p-aminobenzoic acid-n-xyloside sodium salt (k-247) was investigated in the c3h/he mouse-syngeneic tumor system. binding and cytolytic activities of corynebacterium parvum-activated macrophages were significantly enhanced when target mm-102 and mh-134 cells were pretreated with k-247 at doses of 200 or 400 micrograms/ml, while thioglycollate-elicited macrophages showed much lower ...	1984	6609891
models of adoptive t-cell-mediated regression of established tumors.		1984	6610533
regulation of mopc 104e by t cells and growth factors induced by c. parvum stimulation.	corynebacterium parvum, known for its ability to retard the growth of experimental neoplasms, was examined for its effect on the growth of murine plasmacytoma mopc 104e in vivo and in vitro. immunostimulation with c. parvum resulted in the accelerated growth of mopc 104e in experimental animals, as measured by the tumor igm production. the acceleration of plasmacytoma growth was seen in all cases where c. parvum was given before or at the time of tumor transplantation. increased proliferation of ...	1984	6610653
studies on natural killer cell activity and the influence of corynebacterium parvum on murine t-cell leukemogenesis induced by butylnitrosourea.	butylnitrosourea (bnu) was used to induce thymic lymphomas in bdf1 mice. during and after the 12-week bnu exposure the spontaneous nk cell activity against yac-1 cells and that arising 4 days after stimulation with corynebacterium parvum (cp) were measured, as were the mitogen responses of splenic t and b cells. in addition to bnu, groups of mice received multiple injections of the interferon inducer cp during or after the bnu exposure period. the results show a slight impairment of the nk cell ...	1984	6610680
abortive ectromelia virus infection in peritoneal macrophages activated by corynebacterium parvum.	we have previously demonstrated that peritoneal macrophages (m phi s) from c3h mice were resistant to in vitro infection by ectromelia virus, following activation by intraperitoneal injection of the immunomodulator corynebacterium parvum. in contrast, resident and mineral oil-elicited m phi s were fully susceptible to virus infection. this report analyzes the infectious cycle of ectromelia virus in c parvum-activated and mineral oil-elicited m phi s and demonstrates that an abortive infection oc ...	1984	6584516
macrophages in resistance to rickettsial infections: protection against lethal rickettsia tsutsugamushi infections by treatment of mice with macrophage-activating agents.	peritoneal macrophages of balb/c and c3h/hen mice activated in vivo by intraperitoneal inoculation of viable mycobacterium bovis strain bcg or the nonliving macrophage-activating agent propionibacterium acnes (corynebacterium parvum), were resistant to infection with rickettsia tsutsugamushi, and they killed bacteria that did gain entry into the intracellular environment of these cells. this macrophage resistance to infection and intracellular destruction of rickettsiae was dependent upon develo ...	1984	6584528
comparison of corynebacterium parvum with freund's complete adjuvant as a potentiator of the immune response to beta-human chorionic gonadotropin linked to tetanus toxoid.	corynebacterium parvum was compared with freund's complete adjuvant (fca) for potentiation of the rabbit immune response to beta-human chorionic gonadotropin linked to tetanus toxoid (beta-hcg-tt). with each adjuvant, antibodies to hcg were detected using passive hemagglutination. higher antibody titers were produced by fca-treated animals. the ability of antisera to beta-hcg-tt to neutralize the biological action of native hcg was determined by the rat uterine weight assay. anti-beta-hcg-tt ser ...	1984	6588767
experimental otitis media in guinea pigs. serum antibody response to bacteroides fragilis, propionibacterium acnes and peptostreptococcus anaerobius upon challenge.	the production of antibodies in serum was studied after inoculation of bacteroides fragilis, propionibacterium acnes or peptostreptococcus anaerobius into the middle ear of guinea pigs. inoculation of b. fragilis resulted in igg and igm serum antibodies at an early stage of the infection, while iga antibodies were seldom detected and at a later stage. in contrast, inoculation of p. acnes or p. anaerobius induced no serum antibody response. of these three species b. fragilis induced the most inte ...	1984	6598260
effect of vaccine prepared from corynebacterium parvum on the growth of gardner lymphosarcoma in c3h strain mice.	the present paper discusses the effect of a vaccine prepared with formol-killed corynebacterium parvum (alias propionibacterium acnes) on the growth of solid gardner lymphosarcoma transplanted on c3h (h-2k) mice. the vaccine was injected at various time intervals prior to, simultaneously with, or following transplantation of the tumor to intact mice or mice immunized with 60co-irradiated gardner tumor cells. the effectivity was evaluated in terms of numbers of mice surviving without tumor 60-day ...	1984	6549499
treatment of c3h mice bearing solid gardner lymphosarcoma with vaccine prepared from corynebacterium parvum combined with methotrexate and alexan.	c3h mice after transplantation of solid gardner lymphosarcoma were injected with methotrexate (mtx) or alexan. the prolonged survival time of mice treated with antimetabolites was in some cases evidently reduced after an additional injection of corynebacterium parvum (c.p.). this reduction did not depend on the sequence of corynebacterial vaccine or mtx injections. significantly better effect of combined treatment with mtx injection and c.p. was found only once in mice preimmunized with the tumo ...	1984	6549500
augmentation of antitumor resistance by a strain of unicellular green algae, chlorella vulgaris.	growth of meth-a tumor in cdf1 mice was inhibited significantly by injection of a hot water extract of a strain of chlorella vulgaris (ce) into the tumor or into the subcutaneous tissue near the tumor. the augmentation of resistance by ce may require the participation of t cells and macrophages, since it was abolished or reduced in athymic nude mice or mice treated with carrageenan, a macrophage blocker. mice treated with ce exhibited antigen-specific augmented resistance against rechallenge wit ...	1984	6565519
muramyl dipeptide is a powerful potentiator of the antitumor action of various tumor-necrotizing agents.	the previously observed potentiation of necrosis and regression of solid immunogenic meth a sarcoma transplants in mice after iv administration of endotoxin by addition of muramyl dipeptide (mdp) in saline was investigated further by varying time and route of administration of both agents. equal potentiation was observed when mdp was administered 4 h before or after endotoxin, but administration 48 h or 24 h before or 24 h after endotoxin had no effect. simultaneous administration of both agents ...	1984	6566601
acne vulgaris. the past. the present and the future.		1985	2939675
imbalanced accumulation of ribosomal rna in macrophages activated in vivo or in vitro to a cytolytic stage.	previous studies have shown that peritoneal murine macrophages activated in vivo and in vitro to a tumoricidal stage have a depressed rate of rna synthesis. in attempting to clarify the differences in rna metabolism between noncytotoxic and tumoricidal macrophages, we have studied the relative accumulation of various species of rna in macrophages activated in vivo and in vitro with the use of agarose gel electrophoresis. macrophages activated in vitro to a cytotoxic stage with supernatants conta ...	1985	3965571
modulation of hepatocyte protein synthesis during co-cultivation with macrophage-rich peritoneal cells in vitro.	the etiology of hepatic failure associated with the multiple-system organ failure syndrome is poorly understood. because of indirect evidence suggesting that macrophages or kupffer's cells may play a role in this phenomenon, macrophage-rich peritoneal cells were co-cultured with isolated rat hepatocytes. following co-culture, the rate of hepatocyte protein synthesis, quantitated by counts per minute of tritiated leucine incorporated into protein, was significantly diminished. this modulation of ...	1985	3977588
enhanced thermal response of a rat sarcoma by corynebacterium parvum.	corynebacterium parvum was investigated in the response of rat mc7 sarcoma to local waterbath hyperthermia. heat treatment of 1-1.5 cm3 foot tumors at 43 degrees c for 2 h resulted in complete regression of 71% of the tumors. the mc7 cure was reduced to 31% when the tumors were heated at 43 degrees c for 1.5 h. c. parvum (700 micrograms, i.v.) when given 1-3 days before tumor heating at 43 degrees c for 1.5 h increased the host phagocytic activity, and the tumor regression from 31% to 65% (p les ...	1985	3978610
treatment of malignant pleural effusions with intrapleural corynebacterium parvum or tetracycline.	thirty two patients with malignant pleural effusion were randomly allocated to treatment with intrapleural corynebacterium parvum or tetracycline hydrochloride in an attempt to prevent symptomatic recurrence of pleural fluid. success in preventing recurrence of fluid at one month, using up to 2 doses of each drug, was 14 of 16 cases for corynebacterium parvum, 5 of 9 for tetracycline given via an intercostal needle, and 6 of 7 for tetracycline given through an intercostal tube. these difference ...	1985	3979476
prevention of tumor metastasis after surgical removal of primary tumor by using in vitro activated macrophages.	in vitro activation of peritoneal macrophages by propionibacterium acnes and its immunotherapeutic potential for inhibiting metastasis was investigated. c3h/hen mouse adherent peritoneal exudate cells as a source of macrophages were treated in vitro with p. acnes. these p. acnes-activated macrophages were tested for tumoricidal activity in vitro and antimetastatic activity in vivo. in vitro cytotoxicity tests revealed that they had potent cytotoxicity. surgical resection of x5563 tumors in synge ...	1985	3981817
photodestruction of propionibacterium acnes porphyrins.	the fluorescence spectra of colonies of propionibacterium acnes were studied under various experimental conditions. the spectra contained peaks at 580 nm and 620 nm. these bands were due to two different components; the 580 nm component was likely to be a metalloporphyrin, and there are indications that the 620 nm component could be a coproporphyrin. the 580 nm fluorescence was destroyed by the combined action of light and oxygen (no destruction under strict anaerobic conditions). a dark period ...	1985	3993179
corynebacterium parvum augments antibody production in splenectomized mice and mice with sham operations.	the antibody response to a variety of antigens, including pneumococcal polysaccharides, is diminished in splenectomized (splx) mice. we investigated the capacity for the biological response modifier corynebacterium parvum to augment antibody production in splx and sham-splx mice inoculated with pneumococcal polysaccharides and tetanus toxoid. as expected, antibody response to tetanus toxoid was similar in both splx mice and sham-splx mice. c. parvum augmented anti-tetanus toxoid antibody in both ...	1985	3997248
production of tumour necrosis factor in listeria monocytogenes-infected animals.	both mice and rabbits injected intravenously with viable listeria monocytogenes and challenged with bacterial lipopolysaccharide release tumour necrosis factor (tnf) into the blood. optimal conditions for production of murine tnf using listeria were established. the cell-kill efficacy of listeria-tnf and of corynebacterium parvum-tnf are comparable. also, the two sera have similar spectra of activity; a wide variety of murine and human tumour cell lines are vulnerable while others are not affect ...	1985	3997317
the quantity and function of pulmonary alveolar macrophages after splenectomy and corynebacterium parvum.	we have previously shown that corynebacterium parvum (c. parvum), a nonspecific immunomodulator, partially protects splenectomized and nonsplenectomized mice when challenged with aerosolized pneumococci. we report here the effects of both splenectomy and c. parvum on the phagocytic function of the lavageable pulmonary alveolar macrophage (pam). groups of young adult male sprague dawley rats underwent splenectomy or sham operation 3 weeks before injection of c. parvum 1.5 mg ip (or saline) per an ...	1985	3999160
role of lipid a of endotoxin in the production of tumour necrosis factor.	bacterial lipopolysaccharide (lps) induces the release of tumour necrosis factor (tnf) into serum of mice previously infected with listeria monocytogenes or immunized with formalin-killed corynebacterium parvum. this release is greatly reduced by neutralisation of lipid a of lps with the antibiotic polymyxin b sulfate. the effect is dose-dependent. base-hydrolysed lps, which is devoid of lipid a, cannot induce tnf release. crude lipid a still retains its ability to induce tnf release but is sign ...	1985	4000132
can technetium-labelled millimicrospheres be used to measure kupffer-cell function? an experimental study.	it has been suggested that sodium pertechnetate 99mtc millimicrospheres can be used to measure kupffer-cell function. we studied animals and humans to show whether the clearance and catabolism of 99mtc-labelled millimicrospheres can be used as a measure of kupffer-cell function. comparison with albumin 125i-microaggregates clearance of human serum albumin failed to demonstrate that they can be used for this purpose. we suggest that their blood clearance is mainly an expression of liver blood flo ...	1985	4006979
ganglioside alterations in stimulated murine macrophages.	a two-dimensional thin-layer chromatographic technique has been used to separate and display gangliosides from murine peritoneal macrophages in different functional states. resident macrophages have a relatively simple ganglioside pattern with about 15 resorcinol-positive spots. gangliosides from resident cells contained mostly (90%) n-glycolylneuraminic acid. thioglycolate-elicited and corynebacterium parvum-activated macrophages have much more complex patterns with about 40 resorcinol-positive ...	1985	4016115
[studies on hepatocellular injury in experimentally induced acute hepatic failure--i. possible involvement of the activated adherent cells].		1985	4021165
synergistic effects of combination sequential immunotherapies in a murine ovarian cancer model.	the antitumor effects of corynebacterium parvum in a murine ovarian teratocarcinoma model depend upon a sequential activation of neutrophils and macrophages within the peritoneal cavity. we studied the sequential administration of biological response modifiers that independently activate each phase of the response. tumor-challenged mice treated by i.p. injection of a pyridine-extracted fraction of cell-free propionibacterium acnes (pa-pe, 1400 micrograms) demonstrated prolonged survival in less ...	1985	4028011
rejection of murine ovarian cancer following treatment with regional immunotherapy: correlations with a neutrophil-mediated activation of cytostatic macrophages.	rejection of the murine ovarian teratocarcinoma (mot) in c3heb/fej mice, following intraperitoneal (ip) treatment with corynebacterium parvum (c. parvum), is abrogated by injections of silica. we, therefore, investigated whether c. parvum-elicited macrophages affect mot targets in vitro. tumor-cytostatic, but not cytolytic, macrophages were detected in normal and tumor-challenged mice treated with c. parvum. the dose responsiveness and kinetics of macrophage activation strongly correlated with t ...	1985	4028167
purification and partial amino acid sequence of rabbit tumor necrosis factor.	good production of tumor necrosis factor (tnf) in the rabbit was obtained using propionibacterium acnes iid 912 as a priming agent and subsequent administration of lipopolysaccharide. the physicochemical characteristics of rabbit tnf were very similar to those of murine tnf. the molecular weight of rabbit tnf was 39,000 as estimated by gel filtration, and 18,000 by sds-page. the isoelectric point was determined as ph 4.0 by isoelectric focusing. rabbit tnf was stable within the ph range of 5.5 t ...	1985	4030139
repression of lymphatic metastasis by a second implant of the same tumor.	previous work in the rat d-12 fibrosarcoma model has shown that the incidence of macroscopic metastasis, located primarily in regional lymph nodes, is markedly increased after surgical removal of a primary subcutaneous tumor implant, in all probability by facilitating the outgrowth of already established micrometastases. the present work shows that a second implant of live d-12 tumor cells into a remote site effectively represses the outgrowth of macroscopic metastases. for the manifestation of ...	1985	4030246
structure of acidic polysaccharide from cell wall of propionibacterium acnes strain c7.	the structure of polysaccharide prepared by lysozyme digestion from the cell wall of propionibacterium acnes strain c7 was examined. the polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminomannuronic acid in a molar ratio of 1:1:0.3:1:2. by smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideoxymannuronic acid [man(nac)2a] by ...	1985	4030744
experimental photoallergy to systemic drugs.	we have induced photoallergy in mice to systemically administered drugs, specifically sulfanilamide and chlorpromazine. mice were photosensitized to systemic sulfanilamide or chlorpromazine by i.p. administration of drug followed by uvb and uva irradiation of shaved flank skin, on two consecutive days. control mice received i.p. drug with no irradiation. in some experiments cyclophosphamide pretreatment, or intradermal corynebacterium parvum (propionibacterium acnes), was administered as an immu ...	1985	4031536
[proliferative response of murine lymphocytes caused by the activity of amphiphilic molecules from propionibacterium acnes].	splenic lymphocytes from mice treated with propionibacterium acnes cells as well as with their cell walls were found to be variably active on the lymphoproliferative responsiveness. furthermore, the effect of these bacterial agents on the ex vivo con a response of the lymphocytes showed a certain stimulation that was higher with oral treatments. in the same conditions the influence of these agents on the lps lymphocytes stimulation was almost without any statistical significance. in vitro blasto ...	1985	4035056
further experience of corynebacterium parvum in malignant pleural effusion.	the outcome of drug induced pleurodesis has been evaluated in a non-randomised retrospective study of 67 patients with recurrent malignant pleural effusions treated during 1976-83. fourteen died within 30 days of treatment. of the remaining 53 patients, 26 studied during 1976-80 were treated with intrapleural mustine hydrochloride, while 27 studied during 1978-83 were treated with intrapleural corynebacterium parvum. there was no significant difference between the groups in age, sex, or tumour t ...	1985	4035618
disappearance and reappearance of resident macrophages: importance in c. parvum-induced tumoricidal activity.	we have investigated the role of resident macrophages in the early tumoricidal response to c. parvum. the bacteria were labeled with fitc and resident cells were labeled in situ with blue fluorescent covaspheres to enable subsequent monitoring of cellular changes by flow cytometry. macrophages disappeared within 5 hr of administration of bacteria. at 24 hr, fibrinous adhesions containing double labeled macrophages were observed at numerous sites on the peritoneum. macrophages associated with lar ...	1985	4038473
[alveolar lymphocyte proliferation in sarcoidosis patients induced by propionibacterium acnes].		1985	4046241
[treatment of neoplastic pleural effusion by intrapleural administration of corynebacterium parvum vaccine].		1985	4047949
the serologic response of patients with stage ii melanoma to allogeneic melanoma cell vaccines.	seventeen patients with stage ii malignant melanoma were treated with vaccines prepared from three allogeneic melanoma cell lines in an attempt to induce a humoral immune response against melanoma cell surface antigens. the patients were free of detectable melanoma at the time of vaccination. vaccines were prepared from three melanoma cell lines that expressed highly restricted melanocyte differentiation antigens. one of these cell lines also expressed an antigen found only on this particular li ...	1985	4052966
[studies on the hepatocellular injury in an experimentally induced acute hepatic failure. ii. changes in spleen cell and peritoneal macrophage functions].		1985	4057657
an immunological follow-up of chronic adnexitis.		1985	4061184
the effect of hemopoietic microenvironment on splenic suppressor macrophages in congenitally anemic mice of genotype sl/sld.	mechanisms underlying mononuclear phagocyte specialization are being probed by studying suppressor macrophages (m phi) as a reference population in mouse models with impaired blood monocyte formation. splenic suppressor m phi, defined by pge-mediated inhibition of con a-induced t lymphocyte proliferation are induced by the i.p. administration of corynebacterium parvum (cp). mice severely depleted of bone marrow and blood monocytes by treatment with 89sr fail to show this suppressor m phi respons ...	1985	4067308
mouse neoplasia and immunity: effects of radiation, hyperthermia, 2-deoxy-d-glucose, and corynebacterium parvum.	radiation (xrt), hyperthermia, 2-deoxy-d-glucose (2dg), and corynebacterium parvum were given in various combinations to balb/c mice injected with herpes virus type 2-transformed (h238) cells. addition of heat significantly increased the antitumor effects of xrt, and the combination of xrt + 2dg + heat resulted in the highest incidence of complete tumor regression. enhanced activity of phytohemagglutinin-responsive t lymphocytes and natural killer cells capable of killing yac-1 tumor cells was n ...	1985	4069554
purification and properties of a proline iminopeptidase from propionibacterium acnes.	proline iminopeptidase was extracted from the cells of a strain of propionibacterium acnes and purified. the molecular weight was estimated to be about 120,000 by sds-polyacrylamide gel electrophoresis. the enzyme showed the highest activity at 50 degrees c-55 degrees c and its optimum ph was found at 7.5-8.0. the enzyme activity was inhibited by p-chloromercuribenzoate, indicating that this peptidase is a sh-enzyme. especially prolyl-glycyl-glycine but also prolyl-proline bonds were hydrolyzed ...	1985	4082822
biological activities of chemically synthesized partial structure analogues of lipid a.	analogues of the nonreducing sugar part of lipid a were chemically synthesized and tested for biological activities such as limulus amebocyte lysate gelation, interferon- and tumor necrosis factor-induction, lethal toxicity in galactosamine-sensitized mice, and pyrogenicity. a 4-o-monophosphorylglucosamine derivative possessing 2-n-3-tetradecanoyl-oxytetradecanoyl and 3-o-tetradecanoyl groups (gla-27) exhibited all activities tested except for pyrogenicity. alteration of the acyl substituents or ...	1985	4086478
a toxin released from variant strains of propionibacterium acnes isolated from patients with kawasaki disease.		1985	4087873
nine-year microflora study of an isolator-maintained immunodeficient child.	a male child, maintained in a controlled environment, was monitored each month for bacteria, yeasts, and filamentous fungi recovered from the mouth, nasal passages, feces, and nine body surface sites. three natural microbial categories became apparent. incident microorganisms were recovered from within the isolator but did not establish permanent residence. of the 53 incident types isolated, 20 were filamentous fungi and 4 were yeasts. some genera, such as fusobacterium, lactobacillus, neisseria ...	1985	4091564
on the role of endogenous interferon in natural and induced antiviral resistance.		1985	2417549
suppression and abrogation of suppression of induced nonspecific cytotoxicity against tumor cells.	fresh spleen cells obtained from normal mice of various strains including athymic mice and nk-less beige mice were found to suppress in a h-2-nonrestricted manner nonspecific cytotoxic activity of in vitro-cultured normal mouse spleen cells or that of nonadherent peritoneal exudate cells from mice treated with propionibacterium acnes. natural killer-insensitive mm2 ascites tumor cells were used as the target of assay of the cytotoxicity. the suppressive activity of fresh spleen cells was not aff ...	1985	2935654
polyacrylamide gel electrophoresis (page) of whole-cell proteins of cutaneous propionibacterium species.	polyacrylamide gel electrophoresis (page) was applied to the study of whole-cell proteins of cutaneous propionibacteria in an attempt to characterise possible protein patterns that may be typical for strains isolated from acne skin. isolates were obtained from the faces of 33 individuals aged 7-16 years. some of these subjects had apparently normal healthy skin, whereas others had acne vulgaris of varying severity. twenty-five facial isolates of propionibacterium acnes and eight of p. granulosum ...	1985	3155801
blepharitis associated with acne rosacea and seborrheic dermatitis.		1985	3156100
induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-d-glucan and other immunomodulators in murine cells.	the cytotoxic activity of polymorphonuclear leukocytes (pmn) against tumor cells induced in vitro by antitumor immunomodulators was examined by a 51cr release cytotoxicity assay. among 28 immunomodulators and other agents thus far tested, only beta-1,3-glucan from alcaligenes faecalis var. myxogenes ifo 13140, bacillus calmette-guérin, propionibacterium acnes, zymosan a, and nocardia cell wall skeleton were found to cause induction. the cytotoxic activity of pmn with the beta-1,3-glucan was very ...	1985	3156669
[effects on the lymphreticular system and inhibition of liver microsomaal cytochrome p-450 in mice administered priopionibacterium acnes (corynebacterium parvum) bacterin].		1985	3157461
acne fulminans with osteolytic lesions.	multifocal lytic bone lesions were found in a patient with a severe form of acne. the clinical presentation was suggestive of "acne fulminans." propionibacterium acnes was isolated from a subculture of bone tissue. the association of acne fulminans and osteolytic lesions is rare and the pathophysiology is unknown. however, the use of corticosteroids for systemic effect combined with local measures seems to give excellent treatment results.	1985	3158287
antibody titers to propionibacterium acnes cell wall carbohydrate in nodulocystic acne patients.	in order to determine which structures in propionibacterium acnes are most antigenic to severe acne patients, we studied the specificity of anti-p. acnes antibodies in serum from 15 nodulocystic acne patients and 5 normals. complement fixation titers to p. acnes cell wall fractions were determined using guinea pig serum as a complement source. the mean titers of patients and normals to whole cells were 39.6 and 3 (p less than 0.1); to crude cell wall, 138 and 8 (p less than 0.01); and to protein ...	1985	3158714
isolation of an acidic polysaccharide antigen from propionibacterium acnes.	an acidic polysaccharide antigen is released from propionibacterium acnes i and ii during growth. the molecular weight of the antigen was heterogeneous and when fractionated on a sepharose cl-6b column, the antigen was detected at kav values of between 0.1 and 1.0. the pi of the antigen was below 3.5. rabbit antiserum raised against purified acidic-polysaccharide agglutinated p. acnes showing that the polysaccharide was a surface antigen. human antibodies towards whole p. acnes-ii organisms were ...	1985	3160311
inhibition of bacterial translocation from the gastrointestinal tract by nonspecific immunostimulation.		1985	3161085
cytotoxic activity of propionibacterium acnes and other skin organisms.	culture supernatants from four species of skin micro-organisms were tested against vero (monkey kidney cells) and skin fibroblasts for cytotoxic activity. cytotoxic activity was produced by the three species of propionibacterium tested (p. acnes, p. avidum and p. granulosum), and this activity was highest when cultures were grown in the presence of glucose. in contrast, staphylococcus epidermidis was devoid of cytotoxic activity whether grown in the presence of glucose or not. the agent responsi ...	1985	3161535
acne-like chronic inflammatory activity of propionibacterium acnes preparations in an animal model: correlation with ability to stimulate the reticuloendothelial system.	the ability of strains and fractions of killed propionibacteria suspensions to produce chronic rat ear inflammation after intradermal injection of 70-micrograms aliquots was highly correlated with production of splenomegaly in the mouse after i.p. injection of 1.4 mg propionibacterium acnes strains cn 6134, vpi 0009, atcc 11828, and ucla sc and n1 produced a 2- to 3-fold increase in rat ear thickness and a 5- to 7-fold increase in mouse spleen weight 15 days post injection. in contrast p. granul ...	1985	3161957
[a case of infectious propionibacterium acnes endocarditis].		1985	3830541
immunogenicity of corynebacterium pseudotuberculosis and the effect of adjuvants in mice.	intraperitoneal inoculation of cf1 mice with 100 micrograms, 200 micrograms, or 300 micrograms whole cell (wc) or 250 micrograms, 500 micrograms, or 1000 micrograms cell wall (cw) of corynebacterium pseudotuberculosis induced varying degrees of protection after intravenous challenge of immunity with 7.2 x 10(4) cfu of c. pseudotuberculosis. generally, the degree of protection increased with the dose of wc or cw. however, intraperitoneal inoculation of mice with 100 micrograms, 200 micrograms, or ...	1985	3837788
[antitumoral activity of various doses of corynebacterium parvum as a function of the age of mice].	the anti-tumor activity of corynebacterium parvum against two different tumours, a mammary carcinoma grafted in c3h mice and a lymphosarcoma grafted in xvii mice, was lower in young and old mice, than in adult ones. in young mice (25 days) of both lines, low doses of c. parvum were more efficient than higher doses. in 12-month old xvii mice a higher survival rate was obtained by increasing the dose of c. parvum administered, whereas in old c3h mice, this phenomenon was not observed. sublethal do ...	1985	3840664
a sustained increase of microsomal heme oxygenase activity following treatment of rats with bacillus calmette-guerin and corynebacterium parvum: its possible relation to the decrease of cytochrome p-450 content.	the alterations of various enzymes responsible for drug metabolism and heme metabolism were examined in the liver of female rats treated with bacillus calmette-guerin (bcg) and corynebacterium parvum (cp). hepatic drug metabolizing enzyme activities and microsomal cytochrome p-450 and b5 content were significantly decreased for up to 15 and 10 d by a single i.v. administration of bcg and cp, respectively. in contrast, microsomal heme oxygenase activity was markedly increased after bcg and cp tre ...	1985	3841363
development of hyporesponsiveness of natural killer cells to augmentation of activity after multiple treatments with biological response modifiers.	four biological response modifiers (brms), mve-2 (maleic anhydride divinyl ether), corynebacterium parvum (c. parvum), polyiclc (polyinosinic:polycytidylic acid stabilized with poly-l-lysine), and mouse alpha beta-interferon (alpha beta-ifn), were tested to assess whether repeated treatments would repeatedly induce or sustain augmented levels of natural killer (nk) cell activity and/or macrophage (m0)-mediated inhibition of tumor cell growth. in contrast to a significant increase in splenic nk a ...	1985	3845849
evaluation of in vivo and in vitro effectivity of immune defense against a spontaneously arising, nonlymphoid rat tumor. i. analysis of natural immune defense.	possible mechanisms of natural defense against a nonimmunogenic, nonlymphoid rat tumor were evaluated in vitro and examined for effectivity in vivo, using bsp6s, the subcutaneously grown transplantation line of a spontaneously arising fibrosarcoma in the bdx rat strain, which is highly susceptible to natural killer (nk) cells and macrophages (mø). the role of nonspecific immune defense in vivo was demonstrated by eliminating nk cells by irradiation and mø by silica treatment. especially after de ...	1985	3847288
tumour necrosis factor and the lysosomal enzymes of macrophages or macrophage-like cell line.	the relationship between tumour necrosis factor (tnf) and macrophages or macrophage-like cell line, especially the lysosomal enzymes was investigated. the serum lysosomal enzymes and ldh activities were increased in proportion to the tnf production even in different strains of mice. lysosomal enzymes and tnf activity were released into the supernatant of the culture medium of macrophage-enriched peritoneal exudate cells (pec) or spleen cells derived from propionibacterium acnes-primed mice after ...	1985	3851696
natural killer inhibitory substance produced by the peritoneal cells of patients with ovarian cancer.	peritoneal cells obtained from 8 patients with minimal residual ovarian cancer produced a substance during in vitro culture that markedly inhibited the expression of natural killer (nk) cell-mediated lysis. its molecular weight was less than 2,000, the same size as the nk-inhibiting substance (nk-is), a similar nk-suppressive molecule produced by the peritoneal cells of rats. human nk-is suppressed the expression of antibody-dependent cell cytotoxicity as well as nk lysis, but it had no effect o ...	1985	3856049
stimulation of proliferation in mixed cultures of mouse tumor cells and nonimmune peritoneal cells. i. occurrence of stimulation and cyclical variation in tumor cell activity.	mixtures of mouse tumor cells and resident peritoneal cells (pc) from nonimmune mice frequently incorporated [methyl-3h]thymidine [( 3h]dthd) in culture to a far greater extent than did either cell population alone. with pc-to-tumor cell ratios of 10:1, stimulation indices of up to 21 were recorded. syngeneic, semisyngeneic, and allogeneic mixtures were active. pc elicited by thioglycollate or proteose-peptone could participate in the reaction. pc from mice injected with corynebacterium parvum a ...	1985	3856066
immunomodulation of nk and adcc by corynebacterium parvum in acute myeloid leukaemia patients.	natural killer (nk)- and antibody dependent cellular cytotoxicity assays were performed using cryopreserved effector cells in aml patients receiving i.c. and s.c. injections of corynebacterium parvum. a dose related increase in nk could be demonstrated with peaks in nk at day 1 with full cp dosage and at day 14 with 50% doses. this increase was attributable to the cp vaccine since normal donors receiving tetanus toxoid or pneumococcal polysaccharide and aml patients randomized not to receive cp ...	1985	3857405
immunologic control of a retrovirus-associated murine adenocarcinoma. vi. augmentation of antibody-dependent killing following quantitative and qualitative changes in host peritoneal cells.	attempts were made to augment the antibody-dependent killing of the ascitic ad755a tumor in vivo to protect c57bl/6j mice against the outgrowth of larger tumor burdens. the lethal dose for this tumor is less than 100 cells, and antibodies contained in a hyperimmune antitumor serum (his) were found to suppress the outgrowth of a maximum of about 5 x 10(5) cells. thioglycollate injected ip increased the number of peritoneal macrophages, potential effectors for antibody-dependent cell-mediated cyto ...	1985	3862902
immunologic control of a retrovirus-associated murine adenocarcinoma. viii. corynebacterium parvum-activated natural killer cells as potent antibody-dependent cell-mediated cytotoxicity effectors.	the antibody-dependent lytic activity of corynebacterium parvum-induced peritoneal exudate cells was examined in vitro by utilizing ad755a tumor targets and a homologous anti-ad755a hyperimmune serum. maximum antibody-dependent cell-mediated cytolysis (adcc) of tumor targets was achieved within 4 hours of incubation. adcc activity was found primarily in the plastic nonadherent cell population and was greatly enriched following removal of phagocytic cells by carbonyl iron. phenotypically, the cel ...	1985	3862904
reduction of serum interleukin-1-like activity after treatment with dexamethasone.	the ability of steroids to modulate the appearance of interleukin-1(il-1) in vivo was evaluated in a model of endotoxin shock. high levels of il-1 were found in serum from a/j mice which were sensitized with p. acnes and challenged with bacterial lipopolysaccharide (lps). the factor appeared in the serum 2-4 hours after lps challenge and was dependent on the period of p. acnes sensitization and the dose of lps. treating the mice with dexamethasone prior to lps challenge resulted in significantly ...	1985	3871468
adoptive immunotherapy of newly induced murine sarcomas.	two newly induced methylcholanthrene sarcomas of c57bl/6 mouse origin were selected for studies of the adoptive immunotherapy of established tumors. the mca 105 and mca 106 tumors, used during their first five transplant generations, possessed weakly immunogenic tumor-associated transplantation antigens as revealed by failure to elicit immunity to reject 10(6) tumor cells by tumor growth and excision. specific immunity to reject a 10(6) tumor cell challenge could be elicited in less than 50% of ...	1985	3872168
the effect of treatment with corynebacterium parvum on the development and growth of experimental hematogenic metastases of schwannoma in the rat.	single i.v. administration of corynebacterium parvum 5 days before i.v. injection of 10(6) tissue cultured syngeneic schwannoma cells in lewis rats resulted in extension of survival time (p less than 0.05). there was a significant decrease in metastatic tumor incidence for lung, heart, and kidney and decreased lung tumor growth with approximately 50% of the lung tumor burden of untreated controls (p less than 0.05). rats treated similarly with c. parvum 10 days after tumor cell injection showed ...	1985	3876679
[functional roles of macrophages in the immunological response to propionibacterium acnes].		1985	3879588
in vitro generation of procoagulant activity by corynebacterium parvum-stimulated mononuclear leukocytes.	thromboembolic complications are known in cancer patients after i.v. administration of corynebacterium parvum. we examined the ability of this organism to induce production of procoagulant activity by human blood mononuclear leukocytes in vitro. after 4 hours incubation corynebacterium parvum was an effective stimulant for mononuclear leukocytes, behaving in the same way as the typical gram-negative bacterium escherichia coli, whereas mononuclear cells incubated with staphylococcus aureus were n ...	1985	3880801
increased sensitivity of corynebacterium parvum-treated mice to toxic effects of indomethacin and lipopolysaccharide.	female balb/c and c3h/hej mice develop increased sensitivity to the toxic effects of indomethacin after injection of nonviable corynebacterium parvum. the increased sensitivity developed within 4 days of intraperitoneal injection of the organisms and started to resolve 14 days after injection. the development of increased sensitivity was dependent on the quantity of organisms injected and the concentration of indomethacin utilized. the effect was not observed when c. parvum-treated animals were ...	1985	3881348
limited reliability of the "negative" two-dimensional echocardiogram in the evaluation of infectious vegetative endocarditis: diagnostic and surgical implications.	three patients with vegetative endocarditis involving a native valve, a mechanical prosthesis and a bioprosthetic valve respectively are presented. each underwent emergency surgical exploration and prosthetic valve replacement based on clinical evidence of vegetative endocarditis after an initial delay following a report of a negative two-dimensional echocardiographic study. these cases emphasize the fact that within the clinical setting in which vegetative endocarditis is strongly suspected a n ...	1985	3881454
adjuvant corynebacterium parvum immunotherapy for squamous cell epitheliomas of the oral cavity, pharynx, and larynx.	patients with primary squamous cell epitheliomas of the oral cavity, pharynx, and larynx were stratified according to stage and site and randomized to receive either intratumoral immunotherapy with corynebacterium parvum followed 2 weeks later by surgery and postoperative c parvum for 2 years or surgery alone. there were 209 patients entered into the trial and 176 were fully evaluable. all prognostic variables were similar between the two groups. there was no difference in disease-free survival ...	1985	3881630
relationship of hypoglycemia to tumor necrosis factor production and antitumor activity: role of glucose, insulin, and macrophages.	the role of hypoglycemia in tumor necrosis factor (tnf) production was examined. tnf was produced from sera of animals presensitized with reticuloendothelial system stimulants after lipopolysaccharide (lps) challenge. blood glucose was strongly reduced during tnf production. glucose administration to presensitized mice (before lps challenge) caused inhibition of tnf production. exogenous insulin injection inhibited tnf production in a dose-related manner. peritoneal exudate cells (pec) from prop ...	1985	3889459
macrophage functions in biozzi mice.	the faster degradation of antigen by macrophages in biozzi low (l) responder mice, compared to biozzi high (h) responder mice, is thought to be responsible for their lower antibody response. we have measured four functions associated with macrophages to see whether macrophages from l mice were generally more active than those from h mice. peritoneal macrophages obtained from normal mice were compared with those from groups of mice given mycobacterium bovis bcg or propionibacterium acnes. cells f ...	1985	3894222
analysis of protective mechanisms against infection by serratia marcescens.	the present paper is concerned with an experimental study of effects of some agents on parameters pertinent to host resistance to infection of serratia marcescens (s. marcescens) which was isolated from a patient. the results obtained are the following: in the control mice injected intravenously with s. marcescens, most of the bacteria were trapped in the liver, spleen and lung, the so-called reticuloendothelial system (res), and the number of bacteria decreased gradually with time. in the kidne ...	1985	3894671
production of tumor necrosis factor in germfree mice.		1985	3895238
quantitative measurements of humoral immune response in mice to a fanft induced bladder tumor.	using unfixed mouse bladder tumor cells (mbt) as target cells and a modified avidin-biotin-complex (abc) method made it possible to detect a humoral immune response in c3h mice with growing mbt tumors. the rise of the serum levels is significant (p less than 0.005) when compared to control animals and correlates with the tumor size. mice with recurrences after surgical removal of the primary tumor had significantly (p less than 0.05) higher serum values than animals without recurrence. purulent ...	1985	3895707
adjuvant therapy of cutaneous malignant melanoma: a critical review.	the emergence of revised definitions for the high-risk patient with cutaneous malignant melanoma prompts us to re-examine the current status of adjuvant therapy in this disease. we wish to address the question, "once a cutaneous melanoma is surgically removed and the patient is currently free of disease but at high risk for metastases, what can be done to prevent recurrence"?	1985	3897817
effects of dilution rate on biomass and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture.	propionibacterium acnes, p. avidum and p. granulosum were grown in continuous culture at a range of dilution rates on a semi-synthetic medium. dilution rates were chosen to allow the bacteria to grow at the same relative growth rates as compared to their respective mumax values. the steady-state levels and production rates of biomass and extracellular enzymes were determined. the lipase and hyaluronate lyase of p. granulosum and the proteolytic activity of p. acnes and p. avidum were growth link ...	1985	3900281
protective effect of lactobacillus casei on pseudomonas aeruginosa infection in mice.	the protective effect of heat-killed lactobacillus casei yit9018 (lc 9018) against pseudomonas aeruginosa infection in mice was compared with that of corynebacterium parvum. survival of mice after intraperitoneal (i.p.) infection with p. aeruginosa was augmented in mice that had been pretreated i.p. with lc 9018 5 days previously. similar treatment of mice with c. parvum, however, was not effective at all. moreover, mice became more susceptible to infection with p. aeruginosa after such treatmen ...	1985	3921464
t cell involvement in production of tumor necrosis factor: reconstitution experiments with nude mice.	in order to investigate the role of t cells in the production of tumor necrosis factor (tnf), a reconstitution experiment was performed with nude mice (balb/c, nu/nu). the results obtained were as follows: 1) the cytotoxic activity of tumor necrosis serum (tns) from balb/c, nu/nu mice treated with propionibacterium acnes-lps was 1/22 of that from balb/c, nu/+ mice. 2) tnf activity increased 14 times in reconstituted nude mice as compared to balb/c, nu/nu mice. 3) the production of the cytotoxic ...	1985	3924713
drug influences on rat hepatic macrophage enzyme production and release in vitro.	drug influences on hepatic macrophage enzyme release have been investigated using a rat model of macrophage recruitment and activation. n-acetyl-glucosaminidase (nag), a lysosomal enzyme, and plasminogen activator (pa), a cytosolic enzyme, have been measured in both cell lysates and supernatants after 24 h in culture. 6-mercaptopurine (6-mp) and azathioprine significantly decreased (p less than 0.03) the enhanced production of nag by recruited macrophages following stimulation in vitro (total na ...	1985	3926509
[effect of c. parvum-activated macrophages on tumor cells: a microcinematographic study].		1985	3928111
decreased production of interferon alpha and interferon gamma in leucocyte cultures of schizophrenic patients.	in a first approach to measure the activity of the interferon system in schizophrenic patients, leucocyte cultures of schizophrenic patients and normal control individuals were set up using a whole blood assay. in this system both lymphoproliferation and the induction of interferon was tested. the lymphoproliferation (lp) test was performed with one bacterial recall antigen (ppd) and four different mitogens (phytohemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm) and a novel mit ...	1985	3929565
cytotoxic factor production by kupffer cells elicited with lactobacillus casei and corynebacterium parvum.	the ability of kupffer cells, spleen macrophages, pulmonary macrophages, and peritoneal macrophages (pm) to produce cytotoxic factor (ctf) was investigated in vitro. the production of ctf by kupffer cells elicited with corynebacterium parvum (cp) or lactobacillus casei yit9018 (lc9018) was higher than that of spleen, pulmonary macrophages, or pm. in addition, oxygen radical (or) production by kupffer cells or pm was measured. the production of or by kupffer cells or pm was significantly augmente ...	1985	3930066
the effect of combination treatment with alpha-difluoromethylornithine and corynebacterium parvum on b16 melanoma growth and tumoricidal effector cell generation in vivo.	the objective of the present investigation was to establish whether a known lymphoreticular-stimulating agent corynebacterium parvum would augment the established antitumor activity of alpha-difluoromethylornithine in vivo. furthermore, since c. parvum is known to boost cell mediated cytotoxicity, the effect of dfmo (dl-alpha-difluoromethylornithine x hcl x h2o) treatment was evaluated on macrophage and natural killer (nk) cell tumoricidal activity. dfmo administered alone, 1% or 2% in drinking ...	1985	3933819
[experimental studies on the amplification of anti-tumor effects using bacterial dna-treated cancer cells, immunoactivators and interferon].		1985	3934297
evaluation of the sterility and stability of insulin from multidose vials used for prolonged periods.	diabetic patients frequently use their insulin vials for prolonged periods, even though antibacterial preservatives used in multidose insulin vials are not required to be effective beyond 28 days. for this reason, we evaluated the antibacterial activity present in multidose insulin vials for up to 50 days. multidose lente insulin vials were inoculated with s. aureus and p. aeruginosa. vials incubated at room temperature (21 degrees c) were sterile by 48 hours, whereas when they were incubated at ...	1985	3935593