Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| altered hypothalamic-pituitary regulation of thyrotropin in male rats during aging. | 1979 | 108086 | |
| acyl-coenzyme-a synthetase i from candida lipolytica. purification, properties and immunochemical studies. | acyl-coenzyme-a synthetase i from candida lipolytica has been purified to homogeneity as evidenced by polyacrylamide gel electrophoresis in the presence and absence of dodecylsulfate as well as by ouchterlony double-diffusion analysis. the purification procedure involves resolution of cellular particles with triton x-100 and chromatography on phosphocellulose, blue-sepharose and sephadex g-100. the purified enzyme exhibits a specific activity of 20--24 u/mg protein at 25 degree c, which is about ... | 1979 | 108099 |
| exclusion of vha and vhy loci expression on individual b cells from normal and vh allotype-suppressed rabbits. | the distribution of two heavy chain subgroups, vha and vhy, on rabbit peripheral blood lymphocytes was examined by double membrane immunofluorescence. fluroescent anti-a1 and anti-y33 were found to react with separate b cell populations; no doubly stained cells were observed. further evidence for the independent expression of genes controlling the vha and vhy subgroups were obtained by neonatal suppression of a 2 or y33 in a2y33/a3y- heterozygous rabbits. suppression of vha did not affect the ex ... | 1979 | 108111 |
| isolation, homogeneity, and properties of core particle from pyocin r1. | by a mild alkaline treatment of pyocin r1, the core particle was released from the contracted sheath. after sucrose density-gradient centrifugation, core-rich fractions were treated with anti-sheath serum and by a second density-gradient centrifugation, purified core particles were isolated. homogeneity of the preparation was confirmed by observation under the electron microscope, immuno-precipitation reaction, and sucrose density-gradient centrifugation. the core particle exhibited a sedimentat ... | 1979 | 106048 |
| low trimethoprim susceptibility of anaerobic bacteria due to insensitive dihydrofolate reductases. | all the 28 bacteroides fragilis strains investigated were susceptible to sulfamethoxazole (minimal inhibitory concentration < 16 mug/ml) and resistant to trimethoprim (tmp; minimal inhibitory concentration > 4 mug/ml). synergism between sulfamethoxazole and tmp was present in all strains at a ratio of 1:1. the few clostridia investigated proved more resistant to both compounds. dihydrofolate reductases from b. fragilis, c. perfringens, and some other anaerobic species were isolated. inhibition p ... | 1979 | 218496 |
| mechanism of aminoglycoside antibiotic resistance in anaerobic bacteria: clostridium perfringens and bacteroides fragilis. | cell-free amino acid incorporation using ribosomes from strains of either clostridium perfringens or bacteroides fragilis was shown to be susceptible to inhibition by streptomycin and gentamicin. ribosomes bound dihydrostreptomycin as effectively as ribosomes from escherichia coli. no inactivation of streptomycin or gentamicin was detected by cell extracts of either anaerobic bacterial species. b. fragilis, grown without added hemin, menadione, and fumarate, and c. perfringens did not show any t ... | 1979 | 218500 |
| presence of viral particles in the salivary gland of calomys musculinus infected with junin virus by a natural route. | calomys musculinus, a wild cricetid rodent, is one of the main reservoirs of junin virus. six of these animals were infected by being placed in close contact with animals that had been experimentally infected with the virus. they were sacrificed at 10, 15 and 20 months after contact, and their salivary glands were studied by ultrastructural, immunohistochemical and virological methods. two animals developed chronic viremia and low titers of complement-fixing antibodies. these animals were the on ... | 1979 | 218895 |
| recognition of viral antigens in 6/94 virus-induced t-cell-mediated cytotoxicity. | distinct events in the virus-stimulated t-cell-mediated cytotoxicity (v-cmc) have been investigated: 1.) the induction of v-cmc is possible by immunizing mice with infectious as well as uv-inactivated virus (parainfluenza type 1 strain 6/94), or with virus-infected cells either compatible or imcompatible with the recipient. 2). recognition of viral antigens by the effector cells occurs independently of the h2 environment: fractionation of effector cells on columns loaded with virus-infected cell ... | 1979 | 219322 |
| inconsistency of collagenase activity for isolation of islet cells for transplantation. | 1979 | 219568 | |
| characterization of a murine myeloma (mopc-315) c-type virus by nucleic acid hybridization. | 1979 | 219600 | |
| intestinal strangulation in germfree and monocontaminated dogs. | earlier studies in the germfree rat demonstrated that the common intestinal bacteria vary greatly in their ability to cause death after intestinal strangulation. some of these experiments were repeated in adult, germfree and monocontaminated beagles. neither short closed-loop hemorrhagic nor long closed-loop ischemic strangulation killed the germfree dog. either procedure rapidly killed the dog with a conventional bacterial flora or a dog monocontaminated with clostridium perfringens. the dogs m ... | 1979 | 219816 |
| experimental bacteroides fragilis abscess in the rat liver. | liver abscess was induced in rats by injecting a culture of b. fragilis ss. fragilis (approx. 5x10(6) cells) into devascularized liver tissue. mixed infection with b. fragilis and aerobes occurred in all animals subjected to repeated laparotomies for bacteriological sampling (4, 7, 14 and 28 days after inoculation). in animals sampled only at 8 or at 42 days after injection, b. fragilis was isolated in pure culture from the majority of animals. the size of abscesses containing b. fragilis in pur ... | 1979 | 288164 |
| the humoral antibody response to bacteroides fragilis infections in humans. | the humoral antibody response to bacteroides fragilis infections in humans, with particular reference to ss. fragilis, was studied using an enzyme immunosorbent assay (eia). phenol-water extracted polysaccharide fractions (ps) from b. fragilis ss. fragilis, ss. ovatus, ss. distasonis and ss. vulgatus were used as antigens. antibody titer determinations were done on sera collected from 57 patients where b. fragilis had been cultured and from 50 controls. in patients with septicemia caused by b. f ... | 1979 | 288165 |
| anaerobic bacteria in maxillary sinusitis. | the anaerobic bacterial flora of purulent maxillary sinusitis was investigated in 30 patients and the different anaerobic species identified and tested with respect to antibiotic susceptibility. most strains were identified as anaerobic cocci. non-sporeforming gram-positive rods and gram-negative rods other than bacteroides fragilis were also isolated. the majority of the strains were susceptible to benzylpenicillin, cefoxitin, clindamycin, doxycycline, metronidazole and chloramphenicol. | 1979 | 288166 |
| alterations of host defenses paralleling cholesterol-induced atherogenesis. i. interactions of prolonged experimental hypercholesterolemia and infections. | cholesterol may interfere with normal repair processes in the arterial wall. it is also possible that this same action occurs during inflammation and repair throughout the body. this hypothesis was tested by giving intravenous injections of bacteroides fragilis, a bacterium that is innocuous for normal rabbits even when multiple large doses are given. almost all cholesterol-fed rabbits developed significant pleuropulmonary infections with complications that included vasculitis, thrombosis, extra ... | 1979 | 290725 |
| effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice. | the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ... | 1979 | 292809 |
| effects of herbs and drugs during pregnancy and lactation. | prior to considering the effects of herbs and drugs during pregnancy and lactation, attention is directed to the relevance of herbal medicines to human patients. when a drug is taken orally, its absorption, biotransformation, and excretion will affect its blood level. it then makes its way to the capillaries surrounding the alveoli of the breast, and the drug must cross the endothelium of the capillaries into the alveolar cells of the breast and then be excreted into the lumen with the milk. ... | 1979 | 262485 |
| experimental studies on the biological properties of bordetella pertussis. | 1979 | 262507 | |
| recent trends in salmonella and shigella in new york city and at kings county hospital. | 1979 | 283866 | |
| fractionation of glucans synthesized by streptococcus mutans in vitro. | 1979 | 283884 | |
| collagen reduces glycosaminoglycan degradation by cultured mammary epithelial cells: possible mechanism for basal lamina formation. | collagenous substrates are reported to promote the accumulation of extracellular matrix materials by epithelia in culture. glycosaminoglycan (gag) metabolism is compared in secondary cultures of mouse mammary epithelial cells maintained on plastic or type i collagen gel substrates. the incorporation of 35so42- into gag during brief labeling indicates no difference between substrates in the rate of gag synthesis. during prolonged labeling, however, accumulation of [35s]gag in cultures on colllage ... | 1979 | 284399 |
| [comparison of the antibacterial activity of ticarcillin with other antibacterial agents (author's transl)]. | antibacterial activity of ticarcillin was determined in comparison with that of sulbenicillin, amoxicillin, cefuroxime, clindamycin and metronidazole against anaerobic bacteria which have been isolated from various clinical materials in this hospital. growth of more than 90% of gram-negative anaerobic rod bacteria was inhibited by ticarcillin at its concentration of 100 micrograms/ml. strains resistant to ticarcillin showed cross resistance against both sulbenicillin and amoxicillin. antibacteri ... | 1979 | 258675 |
| ornidazole and anaerobic bacteria: in vitro sensitivity and effects on wound infections after appendectomy. | the sensitivities of 68 clinical isolates of bacteroides fragilis, 18 of clostridium perfringens, and 11 of other clostridium species were tested against ornidazole alone and in combination with ampicillin and gentamicin. a concentration of 3.1 microgram of ornidazole/ml inhibited 98% of the strains of b. fragilis, with greater sensitivity when ampicillin and gentamicin were also present. a concentration of 6.2 microgram of ornidazole/ml inhibited 16 of 18 strains of c. perfringens and all 11 st ... | 1979 | 220342 |
| metronidazole bioassay with increased sensitivity. | the bioassay of metronidazole using clostridium butyricum incorporated in agar plates detected concentrations higher than 1.0 microgram/ml. gentamicin in the sample did not affect the growth of the target organism or the inhibition by metronidazole. penicillin in the sample could be eliminated by the incorporation of penicillinase in the agar or by using as the target organism a surface inoculum of the penicillin-resistant bacteroides fragilis. increasing duration and temperature of aerobic pred ... | 1979 | 220515 |
| concomitant t- and tk-activation associated with acquired-b antigens. | three patients with acquired-b antigens are reported whose red blood cells also manifested t- and tk-activation. the results of tests using purified arachis hypogaea and bandeiraea simplicifolia (bs ii) lectins, and red blood cells either t, tk or both t and tk-activated by bacterial enzymes in vitro, suggest that the acquired-b phenomenon, t-activation and tk-activation result from the action of different bacterial enzymes. | 1979 | 222015 |
| subacute cadmium intoxication in jewelry workers: an evaluation of diagnostic procedures. | an outbreak of cadmium intoxication in a jewelry factory provided an opportunity for evaluating the usefulness of diagnostic procedures used to evaluate human cadmium toxicity. blood cadmium levels in workers exposed to cadmium were higher (.93 micrograms/100ml vs .38 micrograms/100 ml) than in unexposed workers. a dose-response relationship was noted between blood cadmium level and symptom prevalence in four symptoms (dyspnea, chest pain, dysuria, and dizziness). segmental hair analysis reveale ... | 1979 | 222221 |
| investigation of the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease. | the immune response to aerobic and anaerobic intestinal bacteria in a patient with crohn's disease with an intestinal fistula was investigated with various serological techniques. two aerobic bacterial species, e. dispar and p. mirabilis, and four strict anaerobic bacterial species, b. fragilis ss. fragilis, f. varium and two different strains of c. perfringens, were isolated from fistula secretion of the patient. these strains were used as antigens for tube agglutination, passive hemagglutinati ... | 1979 | 223235 |
| reciprocal responses to tilt of neurones within both fore- and hindlimb regions of deiters' nucleus. | in decerebrate cats with cerebellum intact the frequency response of 102 neurons located within the lateral vestibular nucleus (lvn) to sinusoidal stimulation of vestibular receptors was analyzed. positional sensitive units, showing a reciprocal pattern of response to lateral tilting, characterized by an excitation during ipsilateral and a depression during contralateral tilt, were equally found in the rostroventral (forelimb) and dorsocaudal (hindlimb) divisions of the lvn. no unit was found to ... | 1979 | 228242 |
| activity of semisynthetic penicillins and synergism with mecillinam against bacteroides species. | the minimal inhibitory concentrations (mic) of six penicillins (ampicillin, carbenicillin, ticarcillin, piperacillin, mezlocillin, and bay k 4999) against 29 clinical isolates of bacteriodes spp. (including bacteroides fragilis, bacteroides thetaiotaomicron, and bacteroides vulgatus) were determined by an agar dilution method. bay k 4999 was most active, followed in descending order by ampicillin, piperacillin, mezlocillin, ticarcillin, and carbenicillin. mecillinam, a 6 beta-amidino-penicillani ... | 1979 | 228593 |
| interspecific heterokaryons between oocytes and blastomeres of the mouse and the bank vole. | interspecific hybrid cells were produced using sendai-virus-mediated fusion of interphase blastomeres from 2-cell embryos with ovulated oocytes of the mouse and bank vole. under the influence of the cytoplasm of oocytes (arrested in metaphase ii) premature chromosome condensation occurs in the nuclei of blastomeres, thus implying that the cytoplasmic factor which induces chromosome condensation is not species specific, at least not between these two species. | 1979 | 229193 |
| anchorage independence: analysis of factors affecting the growth and colony formation of wild-type and dl 54/59 mutant sv40-transformed lines. | 1979 | 229626 | |
| experimental animal models for anaerobic infections. | an experimental animal model that stimulates the mixed aerobic-anaerobic microflora of intraabdominal sepsis was used to study antimicrobial efficacy in vivo. treatment of infected rats with chloramphenicol resulted in only a modest reduction in the percentage of animals surviving infection with abscesses at necropsy. this unanticipated observation led to further exploration of the predominant anaerobes associated with the experimental infection. in vitro cultures of bacteroides fragilis, suscep ... | 1979 | 232936 |
| characterization of a polyvalent conjugate of bacterroides fragilis by fluorescent antibody staining. | a polyvalent conjugate (fluorescein-isothiocyanate-labeled antibody reagent) prepared for bacteroides fragilis contained conjugates prepared against three strains of b. fragilis. this conjugate stained 93% of the 111 b. fragilis test strains; the other 7% of b. fragilis stained at certain times but not others. the conjugate was specific for b. fragilis, 91 strains of anaerobes other than b. fragilis and aerobes failed to give a positive fluorescence. the advantage of this conjugate is that it pr ... | 1979 | 369356 |
| intravenous metronidazole therapy for bacteroides fragilis meningitis. | a 69-year-old man developed meningitis due to bacteroides fragilis and streptococcus mg-intermedius, which progressed during chloramphenicol and nafcillin therapy to the extent that he seemed near death, with frank pus covering the spinal cord at surgery. treatment with intravenous metronidazole and penicillin g was curative. after multiple trauma, a 20-year-old man developed meningitis due to escherichia coli and b fragilis. he failed to respond to chloramphenicol alone, but responded to combin ... | 1979 | 373128 |
| the anti-endotoxin activity of taurolin in experimental animals. | 1979 | 374333 | |
| protein and ribonucleic acid syntheses in heat-damaged and heat-killed escherichia coli. | protein and ribonucleic acid (rna) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. immediately after heating, cells were incubated in growth media containing either [14c]leucine or [3h]uracil. the labeled cells were subsequently treated with penicillin. viable cells were shown to lyse, and the intact nonviable cells were collected by centrifugation. the results showed that after heating, protein and rna synthesis were rein ... | 1979 | 374390 |
| incorporation of n-acetylglucosamine into the core region of the cell wall lipopolysaccharide of escherichia coli. | 1979 | 374407 | |
| fluorescent vi antibody test in the screening of typhoid carriers. | the comparative efficacies of direct bacterial agglutination and immunofluorescent antibody for the estimation of serum vi antibody were determined in the detection of typhoid carriers. sera from all 12 typhoid carriers gave significant titers of 1/10 or more in the direct bacterial agglutination test; however, 26 of 119 (21.8%) sera from culture-negative individuals were also falsely positive. in the fluorescent vi antibody test, 11 of 12 typhoid carriers showed significant serum antibody level ... | 1979 | 377949 |
| clinical and experimental evaluation of cefoxitin therapy. | 30 patients were treated with i.v. cefoxitin (4-8 g/day), of which 20 had documented infections which included endocarditis (5), lung abscess (4), empyema (4), liver and subhepatic abscess (3), osteomyelitis (3), and pancreatic abscess (1). 14 patients had infections caused by anaerobic bacteria and 5 had endocarditis due to aerobic organisms. all but 2 patients with osteomyelitis of the mandible were cured. adverse reactions were noted in 7 patients, mostly due to drug fever and leukocytosis; o ... | 1979 | 378578 |
| anaerobic infection of the liver and biliary tract in experimental common duct occlusion. | the pathogenicity of single anaerobic, single aerobic, and mixed anaerobic and aerobic bacterial inocula was studied in 3 groups of 12 rabbits with biliary tract obstruction. b. fragilis, ss. fragilis and e. coli serotype 01:k1:h7 were used. the investigation was made 7 days after bacterial inoculation and included bacteriological studies of the liver and bile as well as studies of liver function and morphology of the liver and biliary tract. inoculation of 10(5)--10(6) e. coli did not cause bil ... | 1979 | 379988 |
| acute appendicitis and bacteroides fragilis. | bacteria belonging to the bacteroides fragilis group (b. fragilis, b. ovatus, b. vulgatus, b. distasonis, b. thetaiotaomicron and b. uniformis) were cultured in quantities of less than or equal to 10(5) c.f.u./ml from the excised appendix from 30 patients. twenty-two patients (group i) had an acute purulent appendicitis (three with perforation), four were classified as slightly inflamed cases (group 2) and four had a normal appendix (group 3). the b. fragilis group of bacteria dominated among th ... | 1979 | 379991 |
| [experimental application of calcium phosphate granulate for the substitution of conventional bone transplants (author's transl)]. | autologous spongiosa, a calcium phosphate ceramic and kiel bone chips were implanted in the tibiae of dogs and compared with respect to tissue compatibility and osteogenetic effect. after the ceramic implants and the autologous spongiosa had been left in the tibial fat marrow for six weeks, bone tissue and bone marrow had formed to the same extent around both materials. their stimulating effect on osteogenesis was comparable. in contrast to the ceramic material, together with which they had been ... | 1979 | 380203 |
| the william allan memorial award address: on phosphate transport and genetic screening. "understanding backward--living forward" in human genetics. | 1979 | 380328 | |
| cefamandole therapy in anaerobic infections. | thirty-one adult patients with infections due to anaerobic bacteria were treated with cefamandole. bacteroides fragilis group (17) and bacteroides melaninogenicus (13) were the most frequent anaerobes isolated. duration of therapy varied from 2 to 49 days. results were judged satisfactory in 26 cases, and unsatisfactory in 1 case. four cases could not be evaluated. adverse reactions occurred in 16 patients and included positive direct coombs' test without hemolysis, transient liver function abno ... | 1979 | 380458 |
| clindamycin in treatment of aspiration pneumonia in children. | twenty-eight patients with anaerobic pleuropulmonary infections were treated with clindamycin alone or clindamycin with gentamicin. sixteen of the patients presented with pneumonitis, nine with necrotizing pneumonia, and three with lung abscesses. the average length of treatment was 13.8 days, and the duration of temperature after initiation of therapy was 3.1 days. the predominant isolates were anaerobic gram-positive cocci (23 isolates), bacteroides melaninogenicus (14), bacteroides fragilis ( ... | 1979 | 380459 |
| phenylalanyl-trna synthetase induced conformational change of escherichia coli trna phe. | 1979 | 383142 | |
| an inducible proline transport system in candida albicans. | 1. when candida albicans cells were preincubated with proline or grown in the presence of proline as the sole nitrogen source they exhibited a rapid increase in the influx of proline (the inducible transport system). 2. the induction appeared to be specific for proline and also demonstrated in other candida species. 3. both the inducible and constitutive proline uptake systems exhibited similar characteristic features. 4. the nature of the inducer for proline uptake in c. albicans appeared to be ... | 1979 | 383150 |
| cyclacillin: in vitro activity against aerobic and anaerobic bacteria. | cyclacillin at 50 microgram/ml inhibited streptococci (100%), shigella (72%), and bacteroides (b) fragilis ss fragilis (84%). at 100 microgram/ml it inhibited proteus (75%), b. fragilis (86%), and e. coli (50%). cyclacillin was more effective than ampicillin against beta-lactamase-producing h. influenzae. the activity of cyclacillin was found to be more enhanced when tested in nutrient agar and mueller-hinton agar than in brain heart infusion agar. | 1979 | 383419 |
| use of an immunoperoxidase method for identification of bacteroides fragilis. | an indirect immunoperoxidase (ip) slide test was evaluated for the laboratory identification of bacteroides fragilis. antigen-antibody complexes were detected with goat anti-rabbit immunoglobulin g-peroxidase conjugate with 3-amino-9-ethyl-carbazole as the peroxidase substrate. ninety-one percent of 44 b. fragilis strains tested were ip positive (3+ to 4+ reactions) with greater than or equal to 1:160 dilutions of rabbit antiserum produced against whole cells of b. fragilis atcc 23745. the antis ... | 1979 | 385616 |
| practical technique for quantitating anaerobic bacteria in tissue specimens. | quantitative bacteriology has considerable influence on the care and management of surgical wounds. heretofore, these techniques have been limited to measurements of aerobic bacteria. a technique is reported herein which permits quantitation of obligately anaerobic bacteria in tissue specimens. this technique is easily reproduced in any clinical laboratory and eliminates the need for expensive anaerobic chambers. | 1979 | 385618 |
| identification and separation of thy-1 positive mouse spleen cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. | the expression of the thy-1 antigen on mouse spleen cells responsible for nk activity and adcc was investigated by using a monoclonal igm anti-thy-1.2 antibody. both c-mediated cytotoxicity and the fluorescence-activated cell sorter were used to fractionate cells. the effector cells were found to be heterogeneous in their expression of thy-1. effector cells from nude balb/c mice were predominantly thy-1 positive; some of the nk cells in cba spleens appeared to be thy-1 positive, but at least one ... | 1979 | 387876 |
| human cell membrane components dominant in t cell lineage: identification and characterization of human tl-like antigens. | cell membrane components that contain beta 2-microglobulin were purified from cells of a human t cell-type leukemia cell line, hpb-all. they contained membrane components that have the same molecular size and the same subunit structure as hla(a,b,c) antigens but are separable from the typical beta 2-microglobulin-containing cell membrane components, i.e., the hla (a,b,c) antigens, by xenoantibody reagents. a sensitive radioimmunoassay was constructed for detection of the t cell membrane componen ... | 1979 | 387877 |
| anaerobes in biliary tract infection. | 1979 | 391170 | |
| clinical characteristics of anaerobic bactibilia. | during a two-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic bile cultures were performed in 371 patients and 253 of these were positive. anaerobes were isolated from 100 patients, although only twice in pure culture. only aerobes grew from cultures from 153 patients. one hundred cases of biliary tract infections involving anaerobes and an equal number involving aerobes only were reviewed in order to determine their clinical characterist ... | 1979 | 391171 |
| a modified tube method for the cultivation and enumeration of anaerobic bacteria. | a new type of tube (the lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. the lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. anaerobiosis is achieved through deoxygenation of the deep cylinder of agar during sterilization, a minimum of head space, and use of a reducing agent to absorb oxygen introduced during the inoculation procedure. for several species of clostridiu ... | 1979 | 396020 |
| detection of specific igg antibody in sera from patients infected with bacteroides fragilis by enzyme-linked immunosorbent assay. | during a period of 13 months, 28 serious infections caused by bacteroides were seen in 27 patients. sixteen patients yielded bacteroides fragilis; sera from 13 (81%) of these 16 had increased levels of igg specific for b. fragilis lipopolysaccharide (lps) antigens by enzyme-linked immunosorbent assay (elisa). sera from 20 normal controls did not have increased specific igg. sera from 22 of 23 patients with bacteremia caused by other gram-negative rods also failed to yield increased levels of spe ... | 1979 | 396340 |
| bacteriophages of bacteroides. | sixty-eight bacteriophages specific for nine species (dna homology groups) of bacteroides were isolated from sewage. four distinct morphological types were isolated, three of which had not previously been described. attempts to use these phages to transduce bacteroides fragilis were unsuccessful. a total of 91 phage-susceptible strains of bacteroides were used with these phages in a study of the feasibility of developing a scheme for identification of bacteroides species. blind trials were perfo ... | 1979 | 398578 |
| cefoxitin and cephamycins: microbiological studies. | the cephamycins are a family of beta-lactam antibiotics that are produced by actinomycetes and are structurally similar to the cephalosporins. they are characterized by the presence of a 7-alpha-methoxyl group, which confers unusually high resistance to beta-lactamases. cefoxitin, the first semisynthetic cephamycin, is resistant to almost all beta-lactamases. cefoxitin retains the 3-carbamoyl group of cephamycin c and thus has excellent metabolic stability. cefoxitin is bactericidal and almost d ... | 1979 | 400941 |
| treatment of anaerobic infections with metronidazole. | metronidazole (flagl), a compound widely used in man with minimal side-effects, has been used in the treatment of anaerobic infections caused by bacteroides fragilis and other bacteroides species. seven patients were treated and all were restored to full health. four of them did not respond to lincomycin or clindamycin which so far have been the drugs of choice against anaerobic infections. the reasons for the good results during metronidazole therapy such as the good penetration through the blo ... | 1979 | 419371 |
| studies of the antitumor action of 5-fluorouracil (fu). | 1979 | 420946 | |
| comparison of the membrane composition of spiroplasma citri and the corn stunt spiroplasma. | components of membranes isolated from spiroplasma citri and corn stunt spiroplasma grown at 28 degrees c were analyzed. on a protein basis, lipid phosphorus was lower and cholesterol was higher in s. citri. only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenine dinucleotide diaphorase, and adenosine triphosphatase. | 1979 | 422510 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 15-1979. | 1979 | 423921 | |
| [bacteroides fragilis meningitis and bacteremia. report of a case with otogenic focus and a brief review]. | 1979 | 425199 | |
| factors contributing to resistance to beta-lactam antibiotics in bacteroides fragilis. | fifteen strains of bacteroides fragilis, five highly resistant, five moderately resistant, and five susceptible to benzylpenicillin and cephaloridine, were tested for beta-lactamase production. in the highly resistant and the moderately resistant groups of strains, a correlation between formation of beta-lactamase and minimal inhibitory concentrations was demonstrated. in the presence of the beta-lactamase inhibitors clavulanic acid and cp-45,899 at concentrations of 1 mug/ml, the susceptibility ... | 1979 | 426517 |
| hr 756, a new cephalosporin active against gram-positive and gram-negative aerobic and anaerobic bacteria. | the in vitro activity of hr 756, 7-[2-(2-amino-4-thiazolyl)-2-(z)-(methoximino)acetamido] cephalosporanic acid, was investigated against 659 isolates. hr 756 inhibited neisseria and haemophilus species at concentrations similar to those needed with ampicillin. it inhibited beta-lactamase-producing n. gonorrhoeae and h. influenzae. hr 756 was the most active compound tested against members of the enterobacteriaceae, inhibiting most isolates of escherichia coli, klebsiella pneumoniae, proteus mira ... | 1979 | 426518 |
| n,n-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. | human colon carcinoma cell lines established in this laboratory were treated in vitro with n,n-dimethylformamide. this polar solvent caused morphological changes in the cells as well as alterations in their growth properties. untreated cells had cloning efficiencies of up to 77% in soft agar; treatment with n,n-dimethylformamide resulted in a complete loss of clonogenicity in semisolid medium. growth in the presence of the polar solvent also effected a marked reduction in the tumorigenicity of t ... | 1979 | 427742 |
| incidence and pathological features of spontaneous tumors in athymic nude mice. | systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ... | 1979 | 427772 |
| lipopolysaccharides from pseudomonas maltophilia: structural studies of the side-chain polysaccharide from strain n.c.t.c. 10257. | a lipopolysaccharide has been isolated from pseudomonas maltophilia n.c.t.c. 10257. monosaccharide components identified were l-rhamnose, 3-o-methyl-l-xylose, l-xylose, d-glucose, d-mannose, d-galacturonic acid, 2-amino-2-deoxy-galactose, 2-amino-2-deoxyglucose, and a 3-deoxy-2-octulosonic acid. heptose was absent. in this and other respects, the lipopolysaccharide resembles the corresponding products from xanthomonas species. mild hydrolysis of the lipopolysaccharide with acid, followed by chro ... | 1979 | 427832 |
| occupational chronic exposure to organic solvents. vii. metabolism of toluene in man. | 1979 | 429070 | |
| histochemical and atomic absorption demonstration of trace metal mobilization in the central nervous system and liver of the rat. | histochemical and atomic absorption spectrophotometric analysis of trace metal mobilization caused by the action of ethanol in the central nervous system (cns) and liver of the rat is described. histochemically it has been shown that in all neurons examined (motoneurons, pyramidal and purkinje cells) the trace metals (mainly zn2+ and cu2+) are mobilized. most of the stained materials disappear from the perikaryon of the purkinje cells, while in both the motoneurons and the pyramidal cells the tr ... | 1979 | 429206 |
| the link proteins as specific components of cartilage proteoglycan aggregates in vivo. associative extraction of proteoglycan aggregate from swarm rat chondrosarcoma. | 1979 | 429293 | |
| activation of membrane-bound glucosylceramide: beta-glucosidase in fibroblasts cultured from normal and glucosylceramidotic human skin. | 1979 | 429368 | |
| specific cytosol receptors for 1,25-dihydroxyvitamin d3 in human intestine. | human intestine is shown to contain a specific, high affinity cytosol receptor or binding protein for 1,25-(oh)2-vitamin d3 [1,25 (oh)2d3]. this receptor is a protein which sediments at 3.5s on sucrose gradients containing 0.3 m kcl. the receptor binds 1,25 (oh)2d3 with a kd of approximately 2 x 10(-10) m at 4 degrees c. competition binding studies using structural analogs of 1,25 (oh)2d3 indicate that the relative specificity of the receptor is 1,25 (oh)2d3 greater than 1,24r,25(oh)3d3 greater ... | 1979 | 429514 |
| decreased heat-labile opsonic activity and complement levels associated with evidence of c3 breakdown products in infected pleural effusions. | heat-labile opsonic activity was measured simultaneously in serum and pleural fluid of patients with transudates, infectious exudates (with positive or negative bacterial culture) and neoplastic exudates, using two different complement-dependent phagocytic tests: the killing of staphylococcus aureus wood 46 variant strain (k50 opsonic titers) and the assessment of ingestion rate of endotoxin-coated paraffin particles (oil red 0 uptake test). k50 opsonic titers were lower in culture-positive pleu ... | 1979 | 429556 |
| simulated hyperglycemic hyperosmolar syndrome. impaired insulin and epinephrine effects upon lipolysis in the isolated rat fat cell. | these investigations were designed to evaluate the effect of excess glucose and sodium chloride on lipolysis in the isolated adipocyte under normal and modelled pathological conditions simulating the hyperglycemic hyperosmolar syndrome. isolated rat fat cells were incubated in the presence of various combinations of sodium chloride, glucose, epinephrine, and insulin. lipolysis was measured as glycerol and free fatty acid release, and total medium osmolarity as milliosmoles per liter by freezing ... | 1979 | 429561 |
| preservation of binocularity after monocular deprivation in the striate cortex of kittens treated with 6-hydroxydopamine. | the results of single unit recordings from area 17 of monocularly deprived kittens were compared with similar ones from littermates who had been monocularly lid-sutured for the same period of time, but who had in addition been given intraventricular injections of 6-hydroxydopamine (6-ohda) to deplete brain catecholamines. this visual cortices of all catecholamine-depleted kittens showed high proportions of binocular neurons, in contrast to the control group, a majority of whose visual cortical n ... | 1979 | 429612 |
| behavioral and electrophysiological effects of unilateral optic tract section in ordinary and siamese cats. | in ordinary cats, section of one optic tract produced a complete contralateral hemianopsia in both eyes. single-unit recordings showed a normal representation of the contralateral nasal retina and ipsilateral temporal retina in the sc on the side of the intact optic tract. in addition, in the rostral portion of this sc there was a representation of a small portion of the contralateral temporal retina. this portion was apposed to the vertical meridian and its width was at most 6 degrees. in the a ... | 1979 | 429613 |
| evidence of csf enhancement in the spinal subarachnoid space after intravenous contrast medium administration: is intravenous computer assisted myelography possible? | following intravenous injection of iodinated contrast medium, peripheral enhancement of the area inside the spinal canal, is frequently observed by computed tomography. this may be due, at least in part, to enhancement of the cerebrospinal fluid in the spinal subarachnoid space. our preliminary observations in human patients and in animal experiments are reported. the goal of intravenous computer assisted myelography is worth pursuing. | 1979 | 429634 |
| metabolism of atrazine by the soluble fraction (105000g) from chicken liver homogenates. | 1979 | 429685 | |
| effect of vegetable juices and milk on alkylating activity of n-methyl-n-nitrosourea. | 1979 | 429698 | |
| [a biomechanical study on the fracture treatment--intravital measurement of the strain on an intramedullary nail in the healing process of the femoral fracture in goats (author's transl)]. | there have been many biomechanical studies throughout the world on the fracture treatment. however, only a few were performed under intravital condition. the purpose of the present study is to measure the successive strength changes of the callus after a fracture, in vivo in an animal. method: specifically designed küntscher nails were made to meet the anterior convexity and the size of the medullary canal of the femora of goats. three self-temperature-compensating semi-conductor strain-gauges w ... | 1979 | 429823 |
| effects of dialyzable leukocyte extracts with transfer factor activity on leukocyte migration in vitro. 1. antigen-dependent inhibition and antigen-independent inhibition and enhancement of migration. | the effects of dle containing tfd activity from immune human donors on pbl, obtained from individuals nonresponsive to either ppd or cocci antigen, were evaluated in vitro by the agarose lml technique. several different preparations of dle were employed to evaluate the specificity and reproducibility of the effects: (1) from donors skin test positive to ppd but negative to cocci, (2) from donors skin test negative to ppd but positive to cocci, (3) from donors skin test positive to both antigens, ... | 1979 | 429876 |
| amino acid content of rat renal cortex and the response to in vitro incubation. | the concentration of aspartic acid, threonine, serine, glycine, and alanine is significantly higher in newborn rat renal cortex than in the adult tissue, while phenylalanine and histidine are higher in the adult. when adult cortical slices are placed in bicarbonate buffer at room temperature for 20 min there is a 30-60% decrease in the levels of all amino acids except for lysine, which is slightly higher, and methionine and serine, which do not change. under the same conditions, newborn cortical ... | 1979 | 434215 |
| metronidazole therapy of anaerobic bacteremia, meningitis, and brain abscess. | four patients with bacteroides fragilis bacteremia, one patient with a brain abscess due to bacteroides species, fusobacterium naviforme, and peptostreptococcus species, and an infant with bacteroides species ventriculitis and meningitis were treated with metronidazole. in all cases the anaerobic pathogens were eradicated. five of the six patients recovered. one patient with leukemia in whom b fragilis bacteremia was eradicated by metronidazole treatment subsequently died of pseudomonas aerugino ... | 1979 | 434970 |
| antimicrobial activity of antituberculosis agents against anaerobic bacteria. | anaerobic infections may coexist with tuberculosis, and can be mistaken for one another. the effect of therapy with antituberculosis chemotherapeutic agents against anaerobic bacteria (with the exception of rifampin) is unknown. we therefore examined the in vitro efficacy of certain commonly used antituberculosis agents (rifampin, isoniazid, and ethambutol) against 370 strains of anaerobic bacteria, including 86 isolates of bacteroides fragilis. rifampin at a concentration of 2 microgram/ml inhi ... | 1979 | 436485 |
| growth yields and fermentation balance of bacteroides fragilis cultured in glucose-enriched medium. | bacteroides fragilis is an obligate anaerobic bacterium classified with the gram-negative, non-sporeforming bacilli and is the bacteroides species most frequently isolated from human infections. in the present study, experiments were designed to investigate growth characteristics of b. fragilis in a complex medium. in a minimal defined medium, which was employed for comparison purposes, b. fragilis grew with a generation time of 2 h. growth of the organism in glucose-enriched medium used in the ... | 1979 | 438119 |
| superoxide dismutase and o2 lethality in bacteroides fragilis. | exposure of midlog bacteroides fragils (vpi 2393) to 2% o2-98% n2 caused a three- to fivefold increase in superoxide dismutase specific activity within the cells. the increase in specific activity was completed within 90 min after exposure to oxygen and was dependent upon protein synthesis. cells containing the higher superoxide dismutase level were more resistant to the effects of 5 atm of oxygen tension than were cells containing the lower level of superoxide dismutase but were equally resista ... | 1979 | 438129 |
| plasmid-mediated, transferable resistance to clindamycin and erythromycin in bacteroides fragilis. | a clindamycin- and erythromycin-resistant strain of bacteroides fragilis, tmp10, that had been isolated from a blood culture transferred its drug resistance in a millipore mating procedure to another strain of b. fragilis, tm2000, and to a strain of bacteroides thetaiotaomicron. donor strain tmp10 contained numerous species of extrachromosomal dna ranging from approximately 2 x 10(6) to 20 x 10(6) daltons in molecular weight, as revealed by agarose gel electrophoresis. clindamycin- and erythromy ... | 1979 | 438530 |
| [bacteriological and clinical studies on metronidazole in bacteroides fragilis infections (author's transl)]. | the antimicrobial activity of 24 bacteroides fragilis, 13 bacteroides thetaiotaomicron and 5 bacteroides vulgatus, all of which were recent clinical isolates, was determined by the agar dilution technique. these three species had similar susceptibility patterns, but the b. thetaiotaomicron strains were generally less susceptible than the b. fragilis strains. clindamycin was very active against 81% of the bacteroides strains at 3.1 mcg/ml of less while 21% of the bacteroides strains had mics of 1 ... | 1979 | 439390 |
| encapsulated strains of bacteroides fragilis in clinical specimens. | studies were made of the presence of capsules in 294 strains of the bacteroides fragilis group of bacteria isolated from clinical infections and in 30 strains from the fecal flora using the indian ink and ruthenium red staining methods. a total of 77% of the b. fragilis strains were encapsulated; higher figures were observed in isolates from blood and wound infections. of the b. ovatus, b. distasonis, b. vulgatus, b. uniformis, and b. thetaiotaomicron isolates from clinical infections, 17% were ... | 1979 | 440201 |
| aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study. | aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ... | 1979 | 440845 |
| control of experimental breast cancer by antioestrogenic therapies [proceedings]. | 1979 | 444379 | |
| liposome-incorporated corticosteroids: i. the interaction of liposomal cortisol palmitate with inflammatory synovial membrane. | cortisol palmitate-containing liposomes were stable in rheumatoid synovial fluid at 37 degrees. two hours after the intra-articular injection of such liposomes into experimentally arthritic rabbits relatively high proportions of the steroid and lecithin components were recovered from the synovialis. the level of cortisol in the tissue at this time was inversely related to the chronicity of the inflammation. it was found also that the degree and duration of the anti-inflammatory activity of the l ... | 1979 | 444417 |
| anaerobic bacteremia following suprapubic bladder aspiration. | 1979 | 448482 | |
| erythromycin for anaerobic pleuropulmonary and soft-tissue infections. | erythromycin has been reported to be active in vitro against most anaerobic bacteria. we found it effective in treating 14 of 17 patients with mild to moderately severe anaerobic or mixed aerobic/anaerobic pleuropulmonary of soft-tissue infections when adjunctive measures (eg, drainage, débridement, and the use of additional antibiotics to treat important aerobic pathogens) were employed. erythromycin offers a reasonable therapeutic alternative to penicillin in the treatment of a penicillin-alle ... | 1979 | 448959 |
| common regulatory mechanism of expression and conjugative ability of a tetracycline resistance plasmid in bacteroides fragilis. | 1979 | 450069 | |
| transferable lincosamide-macrolide resistance in bacteroides. | 1979 | 451051 | |
| susceptibility of isolates of bacteroides to the bactericidal activity of normal human serum. | seventy-one strains of species from the bacteroides fragilis group, including 46 isolates of b. fragilis, were tested for susceptibility to the bactericidal effect of serum from healthy subjects. twenty-seven (38%) of the isolates were killed by serum. isolates from feces were significantly more sensitive to serum than were isolates from patients with clinical infections. killing of bacteria required heat-labile serum components and was an exponential function of serum concentration. among the v ... | 1979 | 458196 |
| cefoxitin: single-agent treatment of mixed aerobic-anaerobic pelvic infections. | cefoxitin (mefoxin), a new semisynthetic cephamycin antibiotic, resistant to degradation by beta-lactamase enzymes produced by bacteria. in vitro, cefoxitin is active against virtually all clinically important gram-negative facultative bacteria other than pseudomonas and enterobacter spp., gram-positive aerobic bacteria other than the enterococcus, and clinically important anaerobic organisms, including bacteroides fragilis. this broad antibacterial spectrum suggested that cefoxitin might be an ... | 1979 | 460753 |
| microbiology of superficial and deep tissues in infected diabetic gangrene. | in the current study, it is shown that, in the lesions of diabetic gangrene, there are usually mixed flora of two or more organisms. the organisms isolated from the presenting lesion may only partially reflect the organisms found in the deep tissues. of the 58 cultures from the deep tissues, 11 of the isolates were anaerobes, but those anaerobes were never isolated as a pure culture. bacterial synergism probably plays a role in infected diabetic gangrene. maximizing the reduction of systemic inf ... | 1979 | 462355 |