Publications

distribution and significance of heterotrophic marine bacteria with antibacterial activity.	bacteria with antibacterial activity were isolated from seawater, sediments, phytoplankton, and zooplankton of suruga, sagami, and tokyo bays and from soft corals and sponges collected from the taiwan coast. of the 726 strains isolated, 37 showed antibacterial activity against either vibrio parahaemolyticus (atcc 17802) or staphylococcus aureus (p209). sediment harbored the lowest number of these forms of bacteria, and those from tokyo bay did not show any activity. attached isolates showed grea ...	1987	3435149
re: prophylaxis of gram-negative and gram-positive infections in rodents with three intravenous immunoglobulins and therapy of experimental polymicrobial burn wound sepsis with pseudomonas immunoglobulin and ciprofloxacin.		1987	3436681
antitumor activity of lipopolysaccharide and radio-detoxified lipopolysaccharide of vibrio parahaemolyticus.	the antitumor activity of lipopolysaccharide (lps) and radio-detoxified lps of vibrio parahaemolyticus was tested against s180 cells in swiss mice. the toxicity of the lps was 200 times less than that of salmonella typhimurium lps. the v. parahaemolyticus lps could be detoxified by exposure to gamma radiation. both lps and the irradiated lps exhibited antitumor activity, though the irradiated lps was less effective than the native lps. these observations indicated that exposure to gamma radiatio ...	1987	3437829
[involvement of the growth hormone in rapid increases of the potassium concentration of the serum].	as a rule, the infusion of arginine hydrochloride is followed by an increase in serum potassium. the investigation is concerned with the short-term effect of growth hormone, which can be stimulated by arginine (0.6 g/kg body weight), on potassium concentrations in rats and patients with cerebral death. the administration of human somatotropin causes a significant rise in potassium concentrations in patients with cerebral death (0.4 iu/kg body weight) and in rats (4.0 iu/kg body weight). in compa ...	1987	3439594
destructive chronic borrelia meningoencephalitis in a child untreated for 15 years.	a case of borrelia spirochetosis with recurring episodes of progressively destructive chronic meningoencephalitis, untreated for 15 years, is presented. the patient, now 23 years old, represents an example of the natural course of the disease and may serve as a reminder of the diagnosis in retrospective cases as well as prospectively for acute and chronic states of serous meningitis/meningoencephalitis both in children and adults.	1987	3441752
[results of examining the population in a region with high morbidity for oral mucosal cancer: precancerous changes].	interviewing and examination of a population characterized by a high level of morbidity of oral cavity cancer, supposedly induced by the use of nas, established a high incidence of leukoplakia and preleukoplakia at this site which are identified as precancerous lesions. the highest relative risk of oral cavity leukoplakia was found in persons who both chew nas and smoke tobacco (12.4). relative risk of leukoplakia is also elevated in those who either chew nas (5.6) or smoke cigarettes (7.7). thi ...	1987	2950660
[vibrio parahaemolyticus infectious disease caused by kanagawa phenomenon-negative o3:k6 originated from maldives].		1987	3127502
on the diagnostic significance of cft in respiratory-syncytial virus infection in children over one year old.		1987	3133290
lack of enzyme polymorphism in trypanosoma rangeli stocks from sylvatic and domiciliary transmission cycles in colombia.	although trypanosoma rangeli is biologically and morphologically distinct from trypanosoma cruzi, these two hemoflagellates are epidemiologically linked. we report the results of enzyme electrophoretic studies of t. rangeli stocks isolated from sylvatic and domiciliary rhodnius prolixus, and infected humans inhabiting foci in which t. cruzi was sympatrically transmitted. t. rangeli stocks displayed electrophoretically detectable polymorphism for only a single enzyme, isocitrate dehydrogenase (ic ...	1987	3812885
the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr.	a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo.	1987	2435577
relationship of myocardial metabolism and coronary flow: dependence on extracellular calcium.	the hypothesis that extracellular [ca2+] ([ca2+]e) influences the transmission of vasoregulatory information from the heart to coronary smooth muscle was examined. the isolated, retrogradely perfused rat heart was used to evaluate vascular reactivity to transient infusion of either 0.88 mm amytal (amobarbital), hypoxia (95% n2-5% co2), or 12 microm adenosine in the presence and absence of 2.5 microm verapamil. amytal alone enhanced coronary flow by 78% and lactate efflux by 10-fold, whereas it d ...	1987	2446512
definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides.	experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ...	1987	2446556
properties of the membrane-bound 5'-nucleotidase and utilization of extracellular atp in vibrio parahaemolyticus.	vibrio parahaemolyticus utilized atp, adp or amp as the sole source of carbon. about three times higher activity of membrane-bound 5'-nucleotidase was observed in cells grown in the presence of these nucleotides than in their absence: and therefore the enzyme seems to be inducible. since the 5'-nucleotidase activity could be measured with whole cells, the active site of this enzyme appears to be outwardly oriented. both mg2+ and cl- were required for activity. among the divalent cations tested, ...	1987	2835422
[an epidemic of diarrhea caused by vibrio parahaemolyticus occurring in abidjan in 1985].	the authors report the epidemiological results of an epidemic about 84 choleriform syndrome in abidjan (ivory coast), june 1985.	1987	2836102
reaction of tryptophanyl-trna synthetase from beef pancreas with periodate-oxidized atp.	tryptophanyl-trna synthetase from beef pancreas reacts with periodate-oxidized atp according to biphasic kinetics. a rapid phase involves two groups of the protein, presumably lysine side-chains. the slow phase corresponds to the reaction of a larger number of groups. the time-course of the partial losses of the atp-ppi isotopic exchange and of the aminoacylation activities of the enzyme follow the labelling of the two fast-reacting groups. however, the ability of the enzyme to form a bis(trypto ...	1987	2820730
formation of mboii vectors and cassettes using asymmetric mboii linkers.	class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ...	1987	2820843
formation of mboii vectors and cassettes using asymmetric mboii linkers.	class-iis restriction endonucleases such as mboii cleave dna at a specified distance away from their recognition sequences. this feature was exploited to cleave dna at previously inaccessible locations by preparing special asymmetric linker/adapters containing the mboii recognition sequence. these could be joined to dna fragments and subsequently cleaved by mboii. attachment of a 3' phosphate to one of the two different oligodeoxynucleotides comprising the asymmetric duplex prevented ligation at ...	1987	2820843
type beta transforming growth factor affects adrenocortical cell-differentiated functions.	type beta transforming growth factor (tgf-beta) had no detectable effect on mitogenic activities of bovine adrenocortical cells in culture. however, the presence of tgf-beta (1 ng/ml) in the medium resulted in a striking alteration of adrenocortical cell steroidogenic activities, maximally expressed after 18-20 h of treatment. tgf-treated cells exhibited a basal as well as an adrenocorticotropin-stimulated cortisol production inhibition by an average 50-60%, while camp accumulation in response t ...	1987	2820972
type beta transforming growth factor affects adrenocortical cell-differentiated functions.	type beta transforming growth factor (tgf-beta) had no detectable effect on mitogenic activities of bovine adrenocortical cells in culture. however, the presence of tgf-beta (1 ng/ml) in the medium resulted in a striking alteration of adrenocortical cell steroidogenic activities, maximally expressed after 18-20 h of treatment. tgf-treated cells exhibited a basal as well as an adrenocorticotropin-stimulated cortisol production inhibition by an average 50-60%, while camp accumulation in response t ...	1987	2820972
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.	we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...	1987	2823940
experimental transmission and pathogenesis of immunodeficiency syndrome in cats.	we describe the identification, experimental transmission, and pathogenesis of a naturally occurring powerfully immunosuppressive isolate of feline leukemia virus (designated here as felv-faids) which induces fatal acquired immunodeficiency syndrome (aids) in 100% (25 of 25) of persistently viremic experimentally infected specific pathogen-free (spf) cats after predictable survival periods ranging from less than 3 months (acute immunodeficiency syndrome) to greater than one year (chronic immunod ...	1987	2823940
genetic and physical characterization of proba genes of the marine bacterium vibrio parahaemolyticus.	intracellular proline pools have been implicated in the halotolerance of many organisms. to examine this relationship in a moderately halotolerant marine bacterium, vibrio parahaemolyticus, proline biosynthesis genes were cloned in various plasmids. some genetic and structural properties of those genes were examined. subcloning showed that about 3.1 kilobases of v. parahaemolyticus dna could complement proa and prob but not proc mutations of escherichia coli. the same fragment would also complem ...	1987	2829719
properties of adenosine triphosphate-hydrolyzing enzymes in membrane vesicles of vibrio parahaemolyticus.		1987	2830036
the balans chair and its semi-kneeling position: an ergonomic comparison with the conventional sitting position.	recently, the balans chair has been introduced with claims that, because of its semi-kneeling position, individuals will experience decreased low-back pain (lbp) as well as improvement in circulation. this study investigated the validity of these claims. twenty healthy subjects were randomly assigned to one of two groups. group 1 subjects sat in the balans chair for a 30-minute study period and then sat in a conventional office chair for an additional 30-minute period. group 2 subjects were stud ...	1987	2954222
regulatory role of fcr+ and fcr- monocyte subsets in mycobacterium leprae-induced lymphoproliferative response in vitro.	we investigated nine rhesus monkeys (macaca mulatta) inoculated with mycobacterium leprae and three normal human contacts. peripheral blood monocytes were separated into fc receptor positive (fcr+) and fc receptor negative (fcr-) fractions, and their regulatory role in the lymphoproliferative response in vitro to m. leprae was studied. fcr- monocytes had strong antigen presentation activity and produced no suppressor effect while fcr+ monocytes had weak antigen presentation activity and produced ...	1987	2957129
regulatory role of fcr+ and fcr- monocyte subsets in mycobacterium leprae-induced lymphoproliferative response in vitro.	we investigated nine rhesus monkeys (macaca mulatta) inoculated with mycobacterium leprae and three normal human contacts. peripheral blood monocytes were separated into fc receptor positive (fcr+) and fc receptor negative (fcr-) fractions, and their regulatory role in the lymphoproliferative response in vitro to m. leprae was studied. fcr- monocytes had strong antigen presentation activity and produced no suppressor effect while fcr+ monocytes had weak antigen presentation activity and produced ...	1987	2957129
mechanism of action of somatostatin in the hippocampal slice.		1987	2888131
structural and functional modifications of peripheral large arteries in hypertensive patients.		1987	2889808
gastroenteritis due to kanagawa negative vibrio parahaemolyticus.		1987	2880146
1,4-dithiothreitol-induced changes in histamine h1-agonist efficacy and affinity in the longitudinal smooth muscle of guinea-pig ileum.	the effect of 1,4-dithiothreitol (dtt) on histamine h1-receptor agonist affinity and efficacy has been investigated in longitudinal muscle strips of guinea-pig ileum. exposure of ileal smooth muscle to dtt significantly increased the maximal responses to the partial agonists skf71473 and de-2pea, indicative of an increase in agonist efficacy. this effect was paralleled by a small decrease in ec50 values. in contrast, dtt produced a parallel displacement of the concentration-response curve to the ...	1987	2880626
evidence for neoplastic cell differentiation in mediastinal t lymphoblastic lymphoma.	immunostaining of frozen sections from a mediastinal t lymphoblastic lymphoma (t-ll) revealed the existence of two neoplastic cell populations characterized by different degrees of maturation. several large nodules of 3a1+/t11+/t9+ t6-/t4-/t8-/t3- lymphoid cells, resembling normal early thymocytes, were surrounded by 3a1+/t11+/t9+/t6+/t4+/t8+/t3- cells resembling normal cortical thymocytes. the junctional area between early and cortical lymphocytes was occupied by numerous leu-m3+/pam-1+/dr+ ret ...	1987	3103325
characterization of impermeability variants of pseudomonas aeruginosa isolated during unsuccessful therapy of experimental endocarditis.	we characterized five amikacin-resistant variants of pseudomonas aeruginosa isolated from aortic valve vegetations during unsuccessful therapy of experimental endocarditis. these organisms were cross resistant to other aminoglycosides. no aminoglycoside-modifying enzymes were produced by these strains. however, all five variants demonstrated significant defects in permeability and intracellular uptake of [3h]amikacin when compared with the amikacin-susceptible parental strain (0 to 26% of that o ...	1987	3105442
characterization of impermeability variants of pseudomonas aeruginosa isolated during unsuccessful therapy of experimental endocarditis.	we characterized five amikacin-resistant variants of pseudomonas aeruginosa isolated from aortic valve vegetations during unsuccessful therapy of experimental endocarditis. these organisms were cross resistant to other aminoglycosides. no aminoglycoside-modifying enzymes were produced by these strains. however, all five variants demonstrated significant defects in permeability and intracellular uptake of [3h]amikacin when compared with the amikacin-susceptible parental strain (0 to 26% of that o ...	1987	3105442
salt tolerance of lactose-grown vibrio parahaemolyticus carrying escherichia coli lac genes.	lac- strains of vibrio parahaemolyticus were converted to lac+ on receiving a hybrid plasmid containing the lactose utilization genes of escherichia coli k-12. a v. parahaemolyticus strain containing this hybrid plasmid exhibited optimal growth rates on glucose and other carbon sources in the presence of 0.2 to 0.4 m nacl. growth of the same strain on lactose was inhibited at similar concentrations of nacl. the altered growth rate responses in lactose medium appeared to be attributable to effect ...	1987	3105458
[antimicrobial susceptibility of vibrio parahaemolyticus and vibrio alginolyticus isolated from human feces and foods].		1987	3106535
biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes.	platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ...	1987	3108266
differences between saccharomyces cerevisiae and bacillus subtilis in secretion of human lysozyme.	saccharomyces cerevisiae secreted human lysozyme in the medium as an active form when the signal peptides of chicken lysozyme and a chicken lysozyme-aspergillus awamori glucoamylase hybrid were used, whereas it did not synthesize any human lysozyme protein by using the signal peptide of a. awamori glucoamylase. the secreted lysozyme was easily purified and crystallized. on the other hand, bacillus subtilis secreted an inactive human lysozyme, which seemed to have incorrect disulfide bonds, with ...	1987	3109419
stimulation by hyphopichia burtonii and bacillus amyloliquefaciens of aflatoxin production by aspergillus flavus in irradiated maize and rice grains.	aspergillus flavus was grown on maize and rice extract agars and on irradiated viable cracked maize and rice grains, either in pure culture or in dual culture with wild strains of either hyphopichia burtonii or bacillus amyloliquefaciens. aflatoxin production by a. flavus and its growth and interactions with the other microorganisms were studied at three water activities (aw) (0.98, 0.95, and 0.90) and two temperatures (25 and 16 degrees c). both h. burtonii and b. amyloliquefaciens markedly sti ...	1987	3111368
[drug-resistance and transferable r plasmids in vibrio cholerae o-1 and non o-1, v. fluvialis and v. parahaemolyticus recently isolated from human sources].		1987	3112272
distribution of indicator bacteria and vibrio parahaemolyticus in sewage-polluted intertidal sediments.	the impact of a sewage point source on the bacterial densities in an intertidal mud flat in boston harbor, mass., was investigated. the area, savin hill cove, acts as a receiving basin for a combined storm and sewage outlet (cso). preliminary examination of sediments and overlying water at high tide demonstrated that fecal coliforms were present in sediments at abundances 2 to 4 orders of magnitude higher than in the overlying water column. the following bacterial counts were determined from sed ...	1987	3116932
thromboxane a2 biosynthesis in human disease.	thromboxane a2 (txa2), the predominant cyclooxygenase product of human platelets, is a potent vasoconstrictor and platelet agonist. although its biological properties are readily appreciable in vitro, it has been difficult to define its biological importance in vivo. to a large extent this reflected the problems associated with efforts to monitor biosynthesis of this eicosanoid and the lack of selective pharmacological probes that prevented the synthesis of txa2 or antagonized its biological act ...	1987	3100340
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).	the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...	1987	3030193
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).	the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...	1987	3030193
pharmacological modification of pulmonary vascular injury: possible role of camp.	experiments were designed to test the hypothesis that drugs which increase adenosine 3',5'-cyclic monophosphate (camp) in the lung would prevent the pulmonary hypertension and the increase in vascular permeability caused by the infusion of the oxidant lipid peroxide, tert-butyl hydroperoxide (t-bu-ooh), in isolated rabbit lungs perfused with krebs-henseleit buffer. pretreatment with indomethacin or verapamil was also studied, since these drugs block the increase in pulmonary arterial pressure ca ...	1987	3031002
role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats.	permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ...	1987	3032567
calmodulin in porcine malignant hyperpyrexia.	the anaesthetic complication malignant hyperpyrexia (mh) is due to an elevation of the myoplasmic ca2+ concentration. examination of calmodulin isolated from mh susceptible swine suggests that the disorder in calcium regulation in mh is not due to an abnormality in calmodulin.	1987	3032704
a novel mechanism for utilization of extracellular amp in vibrio parahaemolyticus.	vibrio parahaemolyticus could grow with amp, adp or atp as the sole source of carbon. in the presence of cl-, a membrane-bound cl(-)-dependent 5'-nucleotidase seemed to hydrolyze the nucleotides extracellularly, and then the cells took up the resulting adenosine. in the absence of cl-, although no significant dephosphorylation of the nucleotides occurred, the cells could still grow with amp, but not with adp or atp. moreover, in the presence of cl-, zn2+ inhibited the 5'-nucleotidase, and inhibi ...	1987	3034248
active lambda and kappa antibody gene rearrangement in abelson murine leukemia virus-transformed pre-b cell lines.	the two abelson murine leukemia virus (a-mulv)-transformed cell lines, bm18-4 and abc-1, undergo immunoglobulin l-chain gene recombination during passage in tissue culture. bm18-4 cells are capable of kappa gene recombination, whereas abc-1 cells are capable of both kappa and lambda gene recombination. the expression of h chains is apparently not necessary for continuing l chain gene recombination in either of these cells, although h-chain expression may have been involved in the initiation of l ...	1987	3035056
phosphate regulation of gene expression in vibrio parahaemolyticus.	the synthesis of a major outer membrane protein, ompp, in vibrio parahaemolyticus was induced by growth in media deficient in phosphate. the gene, ompp, encoding this protein was cloned. synthesis of ompp in escherichia coli was regulated by the availability of phosphate, and this control required the function of pho regulatory genes of e. coli. analysis of gene fusion strains constructed by mutagenesis with transposon mini-mulux revealed that ompp was transcriptionally regulated in v. parahaemo ...	1987	3038839
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.	the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...	1987	3038946
valanimycin acts on dna in bacterial cells.		1987	3294774
colocalization of microtubule-associated protein 1a and microtubule-associated protein 2 on neuronal microtubules in situ revealed with double-label immunoelectron microscopy.	microtubule-associated protein 1a (map1a) and microtubule-associated protein 2 (map2) were shown to be colocalized on the same microtubules (mts) within neuronal cytoskeletons by double-label immunoelectron microscopy. to investigate the electron microscopic disposition of map1a and map2 and their relationship to mts in vivo, and to determine whether there are different subsets of mts which specifically bind either map1 or map2, we employed a double-label immunogold procedure on rat cerebella us ...	1987	3294858
x-linked gene expression and x-chromosome inactivation: marsupials, mouse, and man compared.	the existence of paternal x inactivation in australian and american marsupial species suggests that this feature of x-chromosome dosage compensation is not a recent adaptation, but probably predates the evolutionary separation of the australian and american marsupial lineages. although it is theoretically possible that the marsupial system is one of random x inactivation with p greater than 0.99 and q less than 0.01 and dependent on parental source, no instance of random x inactivation (p = q or ...	1987	3298156
comparison between proteinases of human seminal plasma and of sperm origin.	a comparison of the alkaline proteinase activity of human seminal plasma, the seminal non-gamete cellular material and spermatozoa was made by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (gelatin-sds-page) zymography. several major (molecular weights = greater than 56,000) and minor (35,000 to 44,000) bands of proteinase activity were seen in the seminal plasma samples from nonvasectomized and vasectomized, healthy donors. similar activity profiles were observed in the nong ...	1987	3305451
foodborne diseases in india.		1987	3308696
effect of acute renal failure on insulin disposition in the isolated perfused rat kidney.	the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ...	1987	3318499
[the effect of an alpha-glucosidase inhibitor on fatty liver in obesity].		1987	3320414
ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis.	ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ...	1987	3326399
ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis.	ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ...	1987	3326399
[cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes].		1987	3329243
bleomycin increases superoxide production in the most active alveolar macrophage subpopulation.	pig alveolar macrophages were separated into three subpopulations by density centrifugation. the most dense subpopulation of macrophages had the greatest rate of superoxide anion production of 4.4 +/- 2.6 nmol/1 x 10(6) cells/min. bleomycin had its greatest effect on this subpopulation by increasing the rate by nearly 100% in cells stimulated with phorbol myristate acetate or phorbol dibutyrate. bleomycin increased the maximum rate of superoxide production without changing the apparent km of 0.0 ...	1987	2447024
chinese hamster ovary cell lysosomes rapidly exchange contents.	we have used cell fusion to address the question of whether macromolecules are rapidly exchanged between lysosomes. donor cell lysosomes were labeled by the long-term internalization of the fluid-phase pinocytic markers, invertase (sucrase), lucifer yellow, fitc-conjugated dextran, or texas red-conjugated dextran. recipient cells contained lysosomes swollen by long-term internalization of dilute sucrose or marked by an overnight fitc-dextran uptake. cells were incubated for 1 or 2 h in marker-fr ...	1987	2447096
stimulation of macrophages by lipopolysaccharide of vibrio parahaemolyticus.	the effect of lipopolysaccharide (lps) from vibrio parahaemolyticus on the biochemical and phagocytic activities of murine peritoneal macrophages was determined. intraperitoneal treatment with different doses (0.5-25 micrograms) of v. parahaemolyticus lps markedly increased the cellular rna content as well as lysosomal enzyme activities of peritoneal macrophages. the treatment also stimulated the phagocytic activities of macrophages. these observations suggest that v. parahaemolyticus lps causes ...	1987	2449599
[catabolite repression of extracellular amylase synthesis in vibrio parahaemolyticus].		1987	2452258
delayed onset of secondary hypertrophic osteoarthropathy.		1987	204179
team building in a psychiatric hospital.		1987	203685
[albrecht graefe (on the 150th anniversary of his birth)].		1988	358049
platelet adhesiveness in cerebrovascular diseases.		1988	266564
preretirement health planning.		1988	259398
monoclonal antibodies to o4 antigen of vibrio parahaemolyticus.	four monoclonal antibodies were prepared against o4 antigen of vibrio parahaemolyticus. all the antibodies were shown to be specific for o4 antigen by agglutination with heat-killed o-cells of the organism and precipitation with lps preparations. the inhibition experiments of the precipitations with various sugars and oligosaccharides suggested that the combining sites of these hybridoma antibodies were directed to an antigenic determinant structure containing----3 and----6 linked d-glucose, d-g ...	1988	2453784
comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus.	hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ...	1988	2466545
magnetic resonance imaging of ischemic bowel in rabbit model.	acute mesenteric ischemic bowel disease is a common yet complex disorder with high morbidity and mortality rates predominantly caused by delayed diagnosis. we examined the role of magnetic resonance imaging (mri) in the early detection of small bowel ischemia using the rabbit model. surgical ligation of the appropriate arterial vascular supply to the ileum of 10 rabbits produced the ischemic compromise. the animals were imaged at different time intervals after the arterial occlusion. multislice, ...	1988	3343117
complete separation of nine equine oestrogens by high-performance liquid chromatography.		1988	3346348
estrogen- and antiestrogen-induced ornithine decarboxylase activity and uterine growth in the rat.	the estrogen antagonists tamoxifen and monohydroxytamoxifen are also classified as partial estrogen agonists. in infantile rats, estradiol induced a single peak of uterine odc activity at 6h following injection regardless of the extent of induction by various estradiol doses. by contrast, the timing of the odc activity peak induced by tamoxifen and monohydroxytamoxifen was highly dependent upon the dosing conditions and was delayed to 18 h at lower tamoxifen doses. in immature rats, tamoxifen an ...	1988	3347055
[a patient with acute secretory diarrhea caused by an autochthonous infection with vibrio parahaemolyticus].		1988	3347287
a theoretical framework for evaluation and treatment of the victims of child sexual assault by a nonfamily member.	thirty-seven families whose children were victims of sexual abuse by a nonfamily member were evaluated and treated during a nine-month period. the average age of the children was 5.36 years. the youngest children were boys, and all the adolescents were girls. a theoretical framework adapted from ferreira's and byng-hall's work was developed to assess and treat child victims of sexual molestation and their families. the format consists of three concepts. first, the trauma from sexual abuse, being ...	1988	3360096
purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus.	a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ...	1988	3360785
high expression of functional adenovirus dna polymerase and precursor terminal protein using recombinant vaccinia virus.	initiation of adenovirus (ad) dna replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (ptp) and dna polymerase (pol) as well as two nuclear dna-binding proteins from uninfected hela cells are required. biochemical studies on the ptp and dna polymerase proteins separately have been hampered due to their low abundance and their presence as a ptp-pol complex in ad infected cells. we have constructed a genomic sequence containing the large open reading fra ...	1988	3362670
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.	the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...	1988	3366515
time course of adaptation to hypoxia in newborn rats.	in newborn rats after a few minutes of hypoxia, ventilation is similar to the normoxic value. nevertheless, after a few days in hypoxia, newborn rats have a sustained hyperventilation. in this study we examined the time course of the newborn rat's adaptation to hypoxia. measurements of body size, hematocrit, lung and heart mass, and breathing pattern have been performed on newborn rats exposed to hypoxia (10% o2) for different time intervals from 4 to 60 h (hypoxic, h), and on same-age rats grow ...	1988	3370546
occurrence of vibrionaceae in natural and cultivated oyster populations in the pacific northwest.	studies were done to assess the role of oysters from the pacific northwest as a potential source of vibrionaceae. oysters collected from natural and cultivated populations on the british columbia coast were opened using sterile instruments. the gills and oyster meat were each removed, and the meat was cut in half. the gills and the cut surface of the oyster meat were each cultured by inoculating them directly to the surface of agar plating media for the isolation of vibrionaceae. overall, 120 oy ...	1988	3370928
vibrio parahaemolyticus (kanagawa-negative) wound infection in a hospital dietary employee.	vibrio parahaemolyticus is an estuarine bacterium that is a major cause of gastroenteritis worldwide. occasionally, this organism has also been shown to be the causative agent of wound infections. in almost all cases of gastrointestinal disease only strains that produce hemolysin ("kanagawa-positive") are implicated. we have isolated a kanagawa-negative strain of v. parahaemolyticus from serous wound drainage of the infected foot of a hospital dietary employee. to our knowledge, this is only the ...	1988	3370930
effects of lignocaine and bupivacaine on regional myocardial function and coronary blood flow in anaesthetized dogs.	empirical i.v. doses of lignocaine or bupivacaine of equal local anaesthetic potency were administered to halothane-anaesthetized dogs. both local anaesthetics caused the expected depression of global haemodynamic function. regional myocardial systolic shortening was depressed similarly by both agents. regional myocardial dysfunction, seen as post-systolic shortening, occurred to a similar extent with both lignocaine and bupivacaine. coronary blood flow and coronary perfusion pressure were signi ...	1988	3377951
protein kinase c in the developing rat liver, heart and brain.	ontogenic changes of protein kinase c in the rat liver, heart and cerebrum were examined from 17-day gestation until adult. cerebral protein kinase c activity was 19 times less at 17-day fetal as compared to 15-day postnatum or older rats. the enzyme activity in the heart was generally higher in the neonatal period than the adult, but was not correlated with the previously reported alpha 1-adrenergic and cholinergic receptor ontogeny. likewise in the liver, the enzyme activity was not correlated ...	1988	3383720
risk assessment for acquiring meningitis tuberculosis among children not vaccinated with bcg: a case-control study.	this case-control study was conducted to assess the risk, among children aged 0-12 years, of developing meningitis tuberculosis (mt) associated with a lack of intradermal bcg vaccination. cases (45) of mt admitted for treatment at the fundacao benjamin guimaraes hospital (belo horizonte, mg, brazil), from 1975 to 1981, were matched for age at hospitalization, date of hospitalization and nutritional status, with two types of controls--patients with acute diarrhoea (ad) and patients with acute non ...	1988	3384537
postoperative wound infection associated with vibrio parahaemolyticus in a patient without exposure to seawater.	this report describes a case of wound infection associated with vibrio parahaemolyticus. the patient had ingested steamed crabs 7 days before admission for surgical treatment of intestinal obstruction due to colon carcinoma. the vibrio sp. was isolated from postoperative wound drainage as well as from stool. recovery was uneventful.	1988	3384932
progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment.	sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ...	1988	3386246
australian fraud inquiry under way.		1988	3386761
dissociation of hcg-receptor complex and detection of hcg receptors in human chorionic tissues.	we developed a radioreceptor assay (rra) system for investigating occupied hcg receptors by using a rat testis membrane fraction. treatment with dithiothreitol, neuraminidase, and low ph (3.3) buffer partially dissociated hcg from its receptor with dissociation rates of 42.8%, 35.2%, and 68.7%, respectively. specific receptor rebinding was unaffected and showed competitive inhibition of [125i]hcg rebinding by unlabeled hcg. scatchard plot analysis yielded a rebinding km of 1.798-3.496/pm. high c ...	1988	3392440
biological activities of lipid a from vibrio parahaemolyticus: stimulation of murine peritoneal macrophages.	the toxicity and macrophage stimulating property of vibrio parahaemolyticus lipid a was studied. the ld50 dose of lipid a in galactosamine-sensitized mice was found to be 0.6 micrograms when injected intraperitoneally. administration of lipid a resulted in stimulation of peritoneal macrophages as evident by increase in their cellular rna contents and lysosomal enzyme activities. the treatment also caused enhancement in the phagocytic activity of macrophages.	1988	3393096
attachment of a vibrio parahaemolyticus strain to rabbit brush border membranes.	the attachment of a clinical isolate of vibrio parahaemolyticus to brush border membranes isolated from rabbits was studied, using transmission electron microscopy. the bacterial cells adhered to the brush borders, apparently with a small space between the cell wall and the surface of the microvilli. no extracellular structures were seen to be involved in the process of adhesion.	1988	3394451
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase].		1988	3395180
flagellar dynamometer controls swarmer cell differentiation of v. parahaemolyticus.	swarmer cell genes, laf, are induced when v. parahaemolyticus is grown on the surface of solidified media, embedded in solidified media, suspended in viscous media, or agglutinated with antibody in liquid media. these conditions have in common the constraint of the movement of the polar flagellum. to test the hypothesis that the polar flagellum functions as a sensor necessary for control of swarmer cell formation, we have constructed a variety of mutations in genes encoding components of the pol ...	1988	3396074
[allergen analysis of mugwort pollen (artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition].	it could be identified 43 antigens of mugwort pollen (artemisia vulgaris), 31 migrating anodically and 12 cathodically, by means of crossed immunoelectrophoresis (cie). eleven antigens bound human ige-antibodies in crossed radioimmunoelectrophoresis (crie). two major allergens could be found, allergens 23 and 40.- the allergenic activity of mugwort pollen extract was examined by means of rast-inhibition. specificity of this reaction could be determined by a control trial with grass pollen extrac ...	1988	3400558
influence of macrogol bases on the bioavailability of bacampicillin after rectal administration in rabbits.		1988	3416367
experimental contact sensitization with 3,4,5-trichloropyridazine.	the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ...	1988	3416588
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study.	carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ...	1988	3418727
structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands.	continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ...	1988	3165262
characterization of muscarinic receptor subtypes on human peripheral lung.	to better define the role of muscarinic receptors in lung responses and airway diseases, we characterized the binding of the m1-specific antagonist, [3h]pirenzepine (pz), and the nonspecific (m1- and m2-) antagonist, [3h]quinuclidinyl benzilate (qnb), to human peripheral lung tissue. data obtained from 15 different lung specimens showed that the radioligands bound to single high-affinity sites with dissociation constant (kd) values ranging from 1 to 9 nm for [3h]pz and 0.03 to 0.46 nm for [3h]qn ...	1988	3170410