Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| comparative study of serum bactericidal activity of cefotaxime alone or in combination with tobramycin. | the objectives of this study were to investigate the bactericidal activity in serum of cefotaxime alone or in combination with tobramycin against clinical strains and to determine the influence of tobramycin on the pharmacokinetics of cefotaxime. the peak bactericidal activity in serum of cefotaxime alone against klebsiella oxytoca, enterobacter aerogenes, serratia marcescens, pseudomonas cepacia, and listeria monocytogenes varied between 1:4 and 1:256. bactericidal activity could still be detec ... | 1986 | 3087282 |
| in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics. | a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ... | 1986 | 3088346 |
| growth of salmonellas in different enrichment media. | the usefulness of selenite-f (s-f), tetrathionate (mkt) and rappaport-10 (r-10) broths as enrichment media to support growth of salmonellas either alone or in the presence of other competing organisms was studied. their ability to support the growth of stressed salmonellas from water was also investigated. it was observed that r-10 was more inhibitory to competing organisms than mkt and s-f. it strongly inhibited the growth of pseudomonas aeruginosa, citrobacter freundii and proteus vulgaris tho ... | 1986 | 3093439 |
| microcalorimetric studies on the effects of media and environmental conditions on the growth of bacteria. | a study has been made of the effects of media and oxygen tension on the power-time (p-t) traces exhibited by cells of klebsiella aerogenes, staphylococcus aureus and pseudomonas aeruginosa. the p-t traces were more complex in shape when the cells were grown in rich nutrient medium than when grown in chemically-defined glucose-limited medium. there was less waste heat and more biomass was produced during growth in nutrient broth. the oxygen tension had a profound effect on the shape of the p-t tr ... | 1986 | 3097461 |
| expression and purification of glutamine synthetase cloned from bacteroides fragilis. | a glutamine synthetase (gs) gene, glna, from bacteroides fragilis was cloned on a recombinant plasmid pjs139 which enabled escherichia coli glna deletion mutants to utilize (nh4)2so4 as a sole source of nitrogen. dna homology was not detected between the b. fragilis glna gene and the e. coli glna gene. the cloned b fragilis glna gene was expressed from its own promoter and was subject to nitrogen repression in e. coli, but it was not able to activate histidase activity in an e. coli glna ntrb nt ... | 1986 | 2887626 |
| isolation and characterization of the m.eaei modification methylase. | the modification enzyme (m.eaei) corresponding to the restriction endonuclease eaei was partially purified from enterobacter aerogenes pw201. the m.eaei enzyme methylates the innermost cytosine residue in each strand of the family of related sequences that constitute the eaei recognition site to give: 5'-y-g-g-5mc-c-r-3' where 5mc is 5-methylcytosine. m.eaei protects these sites against cleavage by haeiii, and protects overlapping 5'-c-c-g-g-3' sites against cleavage by both hpaii and mspi. | 1986 | 3467722 |
| antimicrobial activity of imipenem against 1386 clinical isolates. | 1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ... | 1986 | 3468319 |
| the influence of the o and k antigens of klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents. | the surface hydrophobicity of klebsiella aerogenes is influenced by the presence of capsular (k) and lipopolysaccharide (o) antigens. loss of both k and o antigens (k-o-), but not the k antigen alone (k-o+), increased surface hydrophobicity and susceptibility to phagocytosis. unheated serum (i.e., containing complement) increased the surface hydrophobicity and phagocytosis of the k-o+ and k-o- strains, but not of the k+o+ encapsulated parent strain. despite the altered susceptibility to phagocyt ... | 1986 | 2419562 |
| cloning, expression, and purification of glutamine synthetase from clostridium acetobutylicum. | a glutamine synthetase (gs) gene, glna, from the gram-positive obligate anaerobe clostridium acetobutylicum was cloned on recombinant plasmid phz200 and enabled escherichia coli glna deletion mutants to utilize (nh(4))(2)so(4) as a sole source of nitrogen. the cloned c. acetobutylicum gene was expressed from a regulatory region contained within the cloned dna fragment. glna expression was subject to nitrogen regulation in e. coli. this cloned glna dna did not enable an e. coli glna ntrb ntrc del ... | 1986 | 16347143 |
| bacterial growth rates and competition affect nodulation and root colonization by rhizobium meliloti. | the addition of streptomycin to nonsterile soil suppressed the numbers of bacterial cells in the rhizosphere of alfalfa (medicago sativa l.) for several days, resulted in the enhanced growth of a streptomycin-resistant strain of rhizobium meliloti, and increased the numbers of nodules on the alfalfa roots. a bacterial mixture inoculated into sterile soil inhibited the colonization of alfalfa roots by r. meliloti, caused a diminution in the number of nodules, and reduced plant growth. enterobacte ... | 1986 | 16347173 |
| comparison of bacterial lipopolysaccharides by high-performance liquid chromatography. | a comparison of lipid-free polysaccharides from gram-negative bacteria was rapidly accomplished by using high-performance liquid chromatography of underivatized hydrolysates. examination of a number of such products revealed that, contrary to earlier reports, xanthomonas campestris lipopolysaccharide contained heptose, together with rhamnose and galactose, but not mannose. the polymers from the methanotrophs "methylomonas albus" and "methylosinus trichosporium" contained heptose and glucose, and ... | 1986 | 16347189 |
| enzymatic production of l-serine with a feedback control system for formaldehyde addition. | serine hydroxymethyltransferase (shmt) in the form of crude extract from a recombinant strain of klebsiella aerogenes was used for the production of l-serine from glycine and formaldehyde (hcho). a stirred tank bio-reactor with a continuous feed of hcho (37%) was employed. since the performance of the serine bioreactor was heavily dependent on how hcho was fed, an automatic feedback control system was developed for hcho delivery utilizing the phenomenon of formol titration. this control procedur ... | 1986 | 18553868 |
| enzymatic production of l-serine. | serine hydroxymethyltransferase (shmt) in the form of crude extract form a recombinant strain of klebsiella aerogenes was used to study the production of l-serine from glycine and formaldehyde (hcho). shmt activity linearly increased with temperature (30-50 degrees c). addition of exogenous cofactors, tetrahydrofolic acid and pyridoxal-phosphate, significantly increased shmt activity. the ph optimum of the shmt catalyzed l-serine synthesis step was between 8.0 and 8.5. the k(m) for glycine was 1 ... | 1986 | 18555404 |
| single-cell protein from methanol with enterobacter aerogenes. | an identifiedenterobacter aerogenesutilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. results indicated that the highest yield and conversion were obtained at 0.5% methanol. the addition of seawater as a source of trace elements has an adverse effect. however, the addition of urea as source of nitrogen enhanced the growth of e. aerogenes. heat shock at 60 degrees c for 1 min followed by incubation at 50 ... | 1987 | 18576425 |
| analysis of cell division gene ftsz (sulb) from gram-negative and gram-positive bacteria. | the ftsz (sulb) gene of escherichia coli codes for a 40,000-dalton protein that carries out a key step in the cell division pathway. the presence of an ftsz gene protein in other bacterial species was examined by a combination of southern blot and western blot analyses. southern blot analysis of genomic restriction digests revealed that many bacteria, including species from six members of the family enterobacteriaceae and from pseudomonas aeruginosa and agrobacterium tumefaciens, contained seque ... | 1987 | 2432055 |
| use of tannic acid for the ultrastructural visualization of periplasm in gram-negative bacteria. | tannic acid mordanting reveals the periplasm, the area between the outer membrane and the inner membrane of gram-negative bacteria, rhizobium sp., escherichia coli and enterobacter aerogenes, as an electron-dense layer continuous with the inner leaflet of the outer membrane. the method involves 18 hr of tannic acid treatment after fixation in aldehyde prior to osmium tetroxide postfixation, followed by conventional electron microscopy. | 1987 | 2440153 |
| comparison of difloxacin, enoxacin, and cefoperazone for treatment of experimental enterobacter aerogenes endocarditis. | this study compared difloxacin administered orally, enoxacin administered orally, and cefoperazone administered intramuscularly for the treatment of experimental enterobacter aerogenes endocarditis. difloxacin significantly reduced bacterial counts of vegetations, as compared with enoxacin and cefoperazone. enoxacin and cefoperazone did not differ significantly. this study demonstrated that difloxacin was significantly more effective than enoxacin and cefoperazone for the treatment of e. aerogen ... | 1987 | 3472489 |
| [clinical laboratory approach for estimating effective administrative dose of ceftizoxime. observation from mic and ceftizoxime disc susceptibility test]. | in vitro activities of ceftizoxime (czx) against 328 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. czx was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis, and proteus vulgaris with mic values below 0.20 microgram/ml. it was also active against serratia marcescens and enterobacter aerogenes with mic85 of 3.13 micrograms/ml. czx was less active against staphylococcus aureus and staphylococcus epidermidis, sh ... | 1987 | 3474430 |
| pqq-linked enzymes in enteric bacteria. | klebsiella aerogenes possesses a pqq-linked glucose dehydrogenase. other members of the enterobacteriaceae, such as escherichia coli, salmonella typhimurium or serratia marcescens are seemingly unable to synthesize pqq, but are able to synthesize the glucose dehydrogenase apoenzyme. the physiological significance of this enzyme is discussed. | 1987 | 2856384 |
| common evolutionary origin of the ilvgmeda attenuation locus and trna(1leu) in escherichia coli. | published sequences of transcripts from ilvgmeda leader regions of several enteric bacteria were compared with published sequences of the trnas from escherichia coli. the analyses revealed homology between the ilvgmeda leader peptide-coding region and trna(1leu) in e. coli, salmonella typhimurium, and klebsiella aerogenes, whereas homology was not present in serratia marcescens and edwardsiella tarda. | 1987 | 3294812 |
| evolution of the enterobacterial sula gene: a component of the sos system encoding an inhibitor of cell division. | the lexa-regulated sula (sfia) gene of escherichia coli encodes an unstable protein which inhibits cell division. by determining the nucleotide sequences of the corresponding genes from the related bacteria salmonella typhimurium, enterobacter aerogenes and serratia marcescens it was found that the regulatory region and the lexa binding site (sos box) have been better conserved during evolution than the coding sequence. the n terminus of the sula protein [amino acid (aa) residues 1-30] has diver ... | 1987 | 3297925 |
| incidence of staphylococcus aureus, coliforms and antibiotic-resistant strains of escherichia coli in rural water supplies in port harcourt. | the bacteriological quality of some rural water supplies in port harcourt was monitored over a 3 month period. the supplies were unsatisfactory as judged by standard plate counts (10(3)/ml) and the presence of presumptive and faecal coliforms and staphylococcus aureus. the recovery of potentially pathogenic bacteria (e.g. pseudomonas aeruginosa) further substantiated the existence of health hazards. the most frequently isolated coliforms were escherichia coli, enterobacter aerogenes and klebsiel ... | 1987 | 3298188 |
| biosynthesis of o-succinylbenzoic acid. i: cell free synthesis of o-succinylbenzoic acid from isochorismic acid in enzyme preparations from vitamin k producing bacteria. | escherichia coli k12 and a mutant of e. coli (viz., an 154) as well as aerobacter aerogenes 62-1 (i.e., klebsiella pneumoniae) were used as sources of the enzyme catalyzing the formation of o-succinylbenzoic acid (osb) from isochorismic acid and alpha-ketoglutaric acid in the presence of thiamine pyrophosphate. the product of the reaction (osb) was identified by hplc before and after derivatization to the methylester, dilactone, and coenzyme a ester. osb synthase and alpha-ketoglutarate dehydrog ... | 1987 | 3300552 |
| electron microscopical investigation of citrate lyase single molecules. | electron micrographs of citrate lyase from rhodopseudomonas gelatinosa and klebsiella aerogenes reveal two characteristic molecular forms. the "basket" form and the "star" form were subjected to two-dimensional image reconstruction using a technique involving averaging of superposed single molecular images after rotational correlation. a three-dimensional image reconstruction shows that the images of these forms can be interconverted by rotation and that they therefore represent different views ... | 1987 | 3304340 |
| the k1 beta-lactamase of klebsiella pneumoniae. | beta-lactamase k1 was purified from klebsiella pneumoniae sc10436. it is very similar to the enzyme produced by klebsiella aerogenes 1082e and described by emanuel, gagnon & waley [biochem. j. (1986) 234, 343-347]. an active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. a cysteine residue was found just before the active-site serine residue. this result could explain the properties of the enzyme after modification by thiol-blocking reagents. the s ... | 1987 | 3307765 |
| isolation from klebsiella and characterization of two rcs genes that activate colanic acid capsular biosynthesis in escherichia coli. | two genes, designated rcsa (regulation of capsule synthesis) and rcsb, that had been cloned from the chromosome of klebsiella aerogenes (k. pneumoniae) capsular serotype k21 were capable of activating expression of colanic acid capsular polysaccharide in escherichia coli k12. the klebsiella rcsa gene encoded a polypeptide of 23 kda that was required for the induction of a mucoid phenotype at less than or equal to 30 degrees c but not at greater than or equal to 37 c. the klebsiella rcsb locus en ... | 1987 | 3309150 |
| evaluation of cellulose acetate/nitrate filters for the study of stallion sperm motility. | stallion semen was diluted in a hepes-supplemented buffer (cm) (10(6) spermatozoa/ml) and placed in the upper well of a sykes-moore chemotaxis chamber. chambers were incubated in a humidified atmosphere (5% co2 in air) at 37 degrees c for 1 and 2 h and spermatozoa were allowed to swim through filters with a mean pore size of 3,5 or 8 micron. spermatozoa entered filters of all three pore sizes. distance travelled was greater for each increase in pore size (p less than 0.01) but did not differ (p ... | 1987 | 3316643 |
| the role of capsular polysaccharide k21b of klebsiella and of the structurally related colanic-acid polysaccharide of escherichia coli in resistance to phagocytosis and serum killing. | the behaviour of strains of klebsiella aerogenes of capsular serotype k21 and strains of escherichia coli producing a structurally related polysaccharide (colanic acid) was analysed by phagocytic and serum-killing assays. the cell-surface characteristics of these strains and of non-capsulate strains derived from them were also investigated by partitioning experiments in aqueous two-polymer phase systems. the possession of k21-type capsule by k. aerogenes or colanic-acid polysaccharide by e. coli ... | 1987 | 3320374 |
| [clinical laboratory approach for estimating the effective administrative dose of latamoxef. significance of a 4-category system interpretation of the latamoxef disc susceptibility test]. | in vitro activities of latamoxef (lmox) against 249 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. lmox was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis (except 2 out of 30 strains) and proteus vulgaris with mic values below 3.13 micrograms/ml. it was also active against enterobacter aerogenes with mic80 of 1.56 micrograms/ml. lmox was less active against serratia marcescens, inhibiting about 43% of strai ... | 1987 | 3327966 |
| ultrasound-guided brain abscess aspiration in neonates. | four cases of brain abscess in neonates are described, diagnosed by ultrasonography and ct. all abscesses were confirmed surgically. one patient was operated on 5 weeks after diagnosis because of initial parental refusal. the etiology in all cases was meningitis superimposed on an hypoxic-ischemic insult. two cases had a single abscess while the other two had multiple lesions. all cases were operated on with intraoperative ultrasound examination through the fontanelle. the case with delayed aspi ... | 1987 | 3329960 |
| entire nucleotide sequence of the pullulanase gene of klebsiella aerogenes w70. | we determined the entire nucleotide sequence of the klebsiella aerogenes w70 pullulanase gene (pula) contained on a 4.2-kilobase-pair fragment of plasmid ppb174. the amino acid composition deduced from an open reading frame of 3,288 base pairs agreed closely with that determined for the intracellular pullalanase. the precursor enzyme consisted of 1,096 amino acid residues and contained a hydrophobic n-terminal signal peptide and the consensus sequence for the bacterial prelipoprotein signal pept ... | 1987 | 3155373 |
| direct evidence for the participation of pyruvate in n-hydroxylation of lysine. | the contribution of pyruvate to the formation of n6-acetyl-n6-hydroxylysine by a cell-free system of aerobacter aerogenes 62-1 involved in the production of the dihydroxamate siderophore, aerobactin, has been assessed by a study of the influence of its analogs as well as of inhibitors of thiamine pyrophosphate-dependent decarboxylation reactions. these studies have provided unequivocal evidence for pyruvate functioning not only as a source of reducing equivalents in the initial step of n-hydroxy ... | 1987 | 3100331 |
| deletions in the tetracycline resistance determinant reduce the thermosensitivity of a trfa(ts) derivative of plasmid rp1 in pseudomonas aeruginosa. | a derivative of the broad-host-range plasmid rp1, pme301, was temperature-sensitive (ts) at 43 degrees c for maintenance in pseudomonas aeruginosa, p. mendocina, klebsiella aerogenes and escherichia coli. in e. coli, the ts defect of pme301 could be complemented in trans by the cloned trfa gene, which is known to be essential for rp1 replication in e. coli and p. aeruginosa. because pme301 expressed a ts phenotype in p. mendocina and k. aerogenes, we assume that the trfa function is also vital i ... | 1987 | 3111504 |
| purification and properties of pyridoxal-5'-phosphate-dependent histidine decarboxylases from klebsiella planticola and enterobacter aerogenes. | histidine decarboxylases from klebsiella planticola and enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from morganella morganii. all three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at ph 6.5, decarboxylated only histidine among the amino acids derived from protein, and were tetramers or dimers of identical subunits. amino-terminal sequences of the three enzymes showed up to 81% homology through residue 33, bu ... | 1987 | 3114230 |
| enumeration of pathogenic bacteria from sewage sludge in kuwait. | domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ... | 1987 | 3121985 |
| an outbreak of serious klebsiella infections related to food blenders. | an investigation, including environmental sampling, was undertaken after four leukaemic patients on the same hospital ward developed serious infections with klebsiella aerogenes, capsular type k14. the source of this organism, common to all four patients, was found to be a food blender used for preparing milk-based drinks on the ward. | 1987 | 2883228 |
| in vitro antimicrobial activity of diethyldithiocarbamate and dimethyldithiocarbamate against methicillin-resistant staphylococcus. | staphylococcus aureus has appeared which is highly resistant to both methicillin and aminoglycosides. current therapy involves long-term intravenous therapy of vancomycin. since vancomycin is currently the only drug used to treat these patients, there is a need to develop additional antimicrobial therapy. the in vitro antimicrobial effect of the metal chelator, diethyldithiocarbamate (ddtc) and its structural analog dimethyldithiocarbamate (dmtc) were investigated. both ddtc and dmtc were effect ... | 1987 | 3037985 |
| protocatechuate is not metabolized via catechol in enterobacter aerogenes. | protocatechuate is generally metabolized in bacteria by direct oxygenative cleavage to produce beta-carboxymuconate. an exception to this pattern has been suggested by reports that protocatechuate might be metabolized by nonoxidative decarboxylation to catechol in enterobacter aerogenes. in the present investigation, analysis of mutant strains indicated that this proposed pathway did not make a significant contribution to protocatechuate metabolism in e. aerogenes because mutations blocking cate ... | 1987 | 3680179 |
| five-year survey of changing patterns of susceptibility of bacterial uropathogens to trimethoprim-sulfamethoxazole and other antimicrobial agents. | we analyzed the antibiotic susceptibility of 5,348 urinary isolates of escherichia coli, "klebsiella aerogenes," and proteus mirabilis grown in three laboratories from 1980 to 1985. a continuous rise in resistance to trimethoprim-sulfamethoxazole was observed; 63% of the strains from inpatients in 1984 and 51% of those from outpatients in 1985 were resistant to this drug. isolates from outpatients in 1985 were mostly susceptible to nitrofurantoin (mean susceptibility, 92%) and to oral cephalospo ... | 1987 | 3551824 |
| antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (bw a509u). | the thymidine analog 3'-azido-3'-deoxythymidine (bw a509u; azidothymidine [azt]) had potent bactericidal activity against many members of the family enterobacteriaceae, including strains of escherichia coli, salmonella typhimurium, klebsiella pneumoniae, shigella flexneri, and enterobacter aerogenes. azt also had bactericidal activity against vibrio cholerae and the fish pathogen vibrio anguillarum. azt had no activity against pseudomonas aeruginosa, gram-positive bacteria, anaerobic bacteria, m ... | 1987 | 3551832 |
| purification and characterization of the nickel-containing multicomponent urease from klebsiella aerogenes. | klebsiella aerogenes urease was purified 1,070-fold with a 25% yield by a simple procedure involving deae-sepharose, phenyl-sepharose, mono q, and superose 6 chromatographies. the enzyme preparation was comprised of three polypeptides with estimated mr = 72,000, 11,000, and 9,000 in a alpha 2 beta 4 gamma 4 quaternary structure. the three components remained associated during native gel electrophoresis, mono q chromatography, and superose 6 chromatography despite the presence of thiols, glycols, ... | 1987 | 3553184 |
| treatment of chronic bacterial prostatitis with ciprofloxacin. results of a one-year follow-up study. | in a one-year follow-up study, short-term therapy with ciprofloxacin proved to be beneficial in the treatment of chronic bacterial prostatitis, particularly in cases of escherichia coli prostatitis. ciprofloxacin therapy of e. coli prostatitis was a complete success in five of 12 patients, a probable success in two, and in one patient the outcome cannot be judged. ciprofloxacin treatment failed in three men, and therapy was discontinued in one patient because of side effects. enterobacter aeroge ... | 1987 | 3555048 |
| oral ciprofloxacin in the treatment of elderly patients with complicated urinary tract infections due to trimethoprim/sulfamethoxazole-resistant bacteria. | the effectiveness and safety of ciprofloxacin, a new quinolone antibiotic, were prospectively evaluated in the treatment of patients with complicated urinary tract infections caused by gram-negative organisms resistant to trimethoprim/sulfamethoxazole. twenty-five elderly (mean age, 70.4 years) patients (24 men and one woman) were enrolled. initial pathogens included pseudomonas aeruginosa (15 isolates), escherichia coli (five isolates), enterobacter aerogenes (one isolate), citrobacter freundii ... | 1987 | 3555051 |
| detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates. | after electrophoresis, active pullulanase bands in acrylamide gels have been detected by overlaying and then incubating the gel on a replica gel containing 2.5% pullulan-reactive red conjugate and 2.1% agar. the enzyme activity is revealed as a clear band against a red background on the replica gel. the sensitivity of the replica plate is such that 0.0012 unit of klebsiella aerogenes pullulanase can be detected easily. this procedure is effective in enzyme screening to distinguish pullulanase fr ... | 1987 | 3555152 |
| biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms. | the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ... | 1987 | 3566774 |
| growth of paramecium tetraurelia in bacterized, monoxenic cultures. | wild type and mutant paramecium tetraurelia were grown in monoxenic cultures by first growing enterobacter aerogenes on a defined medium and then adding the paramecium to the stationary phase bacterial culture. the bacterial growth was proportional to the concentration of the carbon source (citrate), and the paramecium growth was dependent upon both the bacterial density and the starting density of paramecium. the behavior, electrophysiological properties, ciliary lipid composition, and growth c ... | 1987 | 3585813 |
| in vitro activity of ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin. | 805 clinical isolates were investigated for their in vitro sensitivity against ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin in a serial dilution test on solid medium. ro 19-5247 had the strongest activity of all drugs tested against streptococci (except streptococcus faecalis) and was as active as cefaclor and cefalexin against most strains of staphylococcus aureus. ro 19-5247 was the only oral cephalosporin active against bordetella pertussis. it was on average 160 times mo ... | 1987 | 3596808 |
| patients' endogenous flora as the source of "nosocomial" enterobacter in cardiac surgery. | we prospectively studied enterobacter colonization in cardiac surgery patients receiving cefazolin prophylaxis. fifty-eight (67%) of 87 patients became colonized, 28 by the time of admission to a cardiac surgery intensive care unit. enterobacter cloacae was four times more prevalent than enterobacter aerogenes. we found increased enterobacter colonization, after prophylaxis, in 45% of surgery patients. none of 25 control patients, who underwent coronary angioplasty and received no antibiotic pro ... | 1987 | 3598234 |
| the in-vitro activity of cefadroxil, and the interpretation of disc-susceptibility testing. | minimum inhibitory concentrations (mics) of cefadroxil were determined for 749 defined clinically-significant bacteria isolated in a london teaching hospital and for 63 strains from an international collection of gram-negative bacilli. assuming a breakpoint of 16 mg/l, for the hospital isolates 81.8% of gram-negative bacilli and 83.4% of gram-positive cocci were sensitive. no significant difference between in-patient, out-patient or community-acquired isolates was found. ninety-five and a half p ... | 1987 | 3610900 |
| isolation of vibrio fluvialis, and unusual pathogen in acute suppurative cholangitis. | we report a case of acute suppurative cholangitis, from which were isolated enterobacter aerogenes and vibrio fluvialis. there have been no previous reports of the isolation of v. fluvialis in this disease. it is usually found in seawater and various seafoods and may cause acute diarrhea, but this is the first report in which it has been associated with acute suppurative cholangitis. | 1987 | 3631041 |
| part of respiratory nitrate reductase of klebsiella aerogenes is intimately associated with the peptidoglycan. | lysozyme digestion and sonication of sodium dodecyl sulfate (sds)-purified klebsiella aerogenes murein sacculi resulted in the quantitative release of both subunits of nitrate reductase, as well as a number of other cytoplasmic membrane polypeptides (5.2%, by weight, of the total membrane proteins). similar results were obtained after lysozyme digestion of sds-prepared peptidoglycan fragments, which excluded the phenomenon of simple trapping of the polypeptides by the surrounding peptidoglycan m ... | 1987 | 3542973 |
| formation of 6-o-alpha-maltosylcyclomalto-oligosaccharides by transfer action of three debranching enzymes. | o-maltosylcyclomaltohexaoses (g2-cg6) were formed in yields of 24.3 and 23.2 mmol from 40 mmol of alpha-maltosyl fluoride (alpha-g2f) and 90 mmol of cyclomaltohexaose (cg6) by the transfer action of pullulanase from aerobacter aerogenes (a-pullulanase) and isoamylase from pseudomonas amyloderamosa, respectively. these yields were three times that given by pullulanase from bacillus acidopullulyticus (b-pullulanase). the yields of o-maltosylcyclomalto-oligosaccharides were changed according to the ... | 1987 | 3427582 |
| absence of a role for lytic microorganisms in the decline of bacteria andsaccharomyces introduced into soil. | the populations ofklebsieila pneumoniae, escherichia coli, enterobacter aerogenes, andpseudomonas sp. fell following their addition to soil, but species lysing these gram-negative bacteria were not detected. the numbers ofstaphylococcus aureus andmicrococcus flavus fell by more than four orders of magnitude and ofsaccharomyces cerevisiae by more than two orders after their addition to soil. organisms lysing these gram-positive bacteria were present in soil, but their numbers did not increase as ... | 1987 | 24202607 |
| [use of imipenem-cilastatin in neonatal septicemias caused by gram-negative bacilli multiresistant to beta-lactam antibiotics]. | seven neonates with septicemia due to gram negative bacteria resistant to beta-lactam received imipenem-cilastatin therapy. bacteria isolated were enterobacter cloacae [3], enterobacter aerogenes [1], klebsiella pneumoniae [1], serratia marcescens [1], pseudomonas fluorescens [1]. the mics of imipenem were lower 1 microgram/ml. in 3 children septicemia occurred during previous antimicrobial chemotherapy. 3 iv 60 mg/kg doses of imipenem with amikacin (15 mg kg/d) were administered every day. for ... | 1988 | 3043351 |
| a bacterial factor induces changes in cysteine proteinase forms in the cellular slime mould dictyostelium discoideum. | the electrophoretic pattern of cysteine proteinases in axenically grown myxamoebae of dictyostelium discoideum can be altered by the addition of either gram-negative (klebsiella aerogenes, escherichia coli) or gram-positive (micrococcus lysodeikticus, bacillus subtilis) bacteria to the culture. no changes occurred, however, if either yeast or latex beads were used in place of bacteria. the changes involved the simultaneous loss of proteinases characteristic of the axenic cells (the a-forms) and ... | 1988 | 3052431 |
| divergence of the aerobactin iron uptake systems encoded by plasmids pcolv-k30 in escherichia coli k-12 and psmn1 in aerobacter aerogenes 62-1. | although the aerobactin-mediated iron uptake system has been characterized genetically in escherichia coli, the siderophore aerobactin was chemically characterized after purification from culture supernatants of aerobacter aerogenes 62-1, a member of the klebsielleae. we have cloned and mapped the genes encoding the aerobactin system genes of a. aerogenes 62-1 and begun characterization of the relevant proteins and enzymatic activities of this plasmid-mediated aerobactin system. published chemic ... | 1988 | 3053646 |
| evolution of aminobenzoate synthases: nucleotide sequences of salmonella typhimurium and klebsiella aerogenes pabb. | p-aminobenzoate synthase (ps) and anthranilate synthase (as) are structurally related enzymes that catalyze similar reactions and produce similar products, para- and ortho-aminobenzoate (anthranilate). each enzyme is composed of two non-identical subunits: a glutamine amidotransferase subunit (coii) and a subunit that produces an aminobenzoate product (coi). nucleotide sequence comparisons of the escherichia coli genes encoding each of the subunits suggest a common evolutionary origin for both s ... | 1988 | 3057324 |
| effect of subinhibitory concentration of gentamicin on adherence of klebsiella aerogenes to uroepithelial cells in vitro. | 1988 | 3058587 | |
| comparison of broth enrichment and direct plating for the isolation of campylobacter jejuni from dogs. | two techniques for the isolation of campylobacter jejuni from feces, direct plating and thioglycolate broth enrichment, were compared. a total of 272 rectal swab cultures were performed on 156 laboratory dogs. campylobacter blood agar plates and campylobacter thioglycolate broth were inoculated immediately upon sampling of the dogs. after incubation at 4 degrees c for 12 to 16 h, material from the campylobacter thioglycolate medium was inoculated onto campylobacter blood agar plates. a total of ... | 1988 | 3069857 |
| [a specific property of bacteria in the genus klebsiella--a color reaction with 5-aminosalicylic acid in the nutrient medium]. | for the first time bacteria of the genus klebsiella have been found to possess a specific property, characteristic of this genus only, i.e. the capacity of giving color reaction with 5-aminosalicylic acid. this reaction can be observed in all klebsiella species under study: k. pneumoniae (94.4 +/- 2.3%), k. ozaenae (93.3 +/- 4.5%), k. rhinoscleromatis (100%), k. oxytoca (88.0 +/- 4.9%), k. mobilis (92-5 +/- 4.3% of the strains). in all other bacteria under study (40 species, 22 genera and 7 fami ... | 1988 | 3072806 |
| [biochemical typing of enterobacter isolated from several clinical materials]. | a total of 74 enterobacter species have been isolated from the patients applying to the department of microbiology, university of ankara, studying several biochemical test systems their strains have been found. 37 of these enterobacter species have been found to be enterobacter cloacae, 10 enterobacter agglomerans, 13 enterobacter aerogenes, 3 enterobacter hafniae (hafnia alvei), 1 enterobacter sakazakii. 2 of the strains couldn't be classified. in conclusion most of the strains were found to be ... | 1988 | 3078792 |
| bacterial adhesion to intravenous cannulae: influence of implantation in the rabbit and of enzyme treatments. | comparison was made of the adhesion of staphylococcus epidermidis, staphylococcus aureus, escherichia coli, klebsiella aerogenes and pseudomonas aeruginosa to six types of intravascular cannula material. adhesion to materials removed from rabbit tissues did not differ significantly between types of material or between bacterial species. in contrast, major differences were found when unimplanted materials were examined; the overall rank order of adhesiveness of bacteria to unimplanted materials ( ... | 1988 | 3123262 |
| [pseudomonas aeruginosa and surgical intensive care units]. | the prevalence of pseudomonas aeruginosa in intensive care unit (icu) is 19 per cent although its incidence in blood culture is only 3.5 per cent and remains the same even though the total prevalence of this bacterium increases. a review of the clinical results shows that this germ causes severe complications in only 3 per cent of patients. a study by epidemiological markers reveals the presence of 16 different iats serotypes in the icu, this distribution of the serotypes being similar to this o ... | 1988 | 3132871 |
| in vitro evaluation of e1040, a new cephalosporin with potent antipseudomonal activity. | e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent antipseudomonal activity. the compound was four- to eightfold more active than ceftazidime and cefsulodin against pseudomonas aeruginosa (mic of e1040 for 90% of strains tested [mic90], 3.13 micrograms/ml). e1040 also showed a potent activity against other glucose-nonfermentative rods, including acinetobacter species. the activities of e1040 against most species of the family enterobacteriaceae were roughly co ... | 1988 | 3134847 |
| synthesis and structure-activity relationships in the cefpirome series. i. 7-[2-(2-aminothiazol-4-yl)-2-(z)-oxyiminoacetamido]-3-[(su bstituted-1-pyridinio)methyl]ceph-3-em-4-carboxylate s. | 7-[2-(2-aminothiazol-4-yl)-2-(z)-oxyiminoacetamido]-3-[(s ubs tituted-1-pyridinio)methyl]ceph-3-em-4-carboxylates ii are a group of beta-lactam antibiotics with extraordinary high antibacterial activity. the promising member of this group, cefpirome (hr 810, ii-1) is a candidate for clinical use. synthetic pathways to ii starting from cefotaxime derivatives i or 7-aminocephalosporanic acid (7-aca) are described. a preferred method for the conversion of i to ii or 7-aca to precursors iii respecti ... | 1988 | 3142844 |
| regulation of the galactose-inducible lac operon and the histidine utilization operons in pts mutants of klebsiella aerogenes. | galactose appears to be the physiological inducer of the chromosomal lac operon in klebsiella aerogenes. both lactose and galactose are poor inducers in strains having a functional galactose catabolism (gal) operon, but both are excellent inducers in gal mutants. thus the slow growth of k. aerogenes on lactose reflects the rapid degradation of the inducer. several pts mutations were characterized and shown to affect both inducer exclusion and permanent catabolite repression. the beta-galactosida ... | 1988 | 3142852 |
| in vitro activity of florphenicol. | florphenicol was active at a lower concentration than chloramphenicol against over half of 234 recent clinical bacterial isolates. the majority (98%) of the isolates were inhibited by florphenicol at a concentration of 8 mg/l or less. florphenicol was particularly effective against chloramphenicol resistant strains of haemophilus influenzae. klebsiella aerogenes and bacteroides spp. florphenicol was bacteristatic for salmonellae and escherichia coli but bactericidal for haemophilus influenzae. f ... | 1988 | 3143587 |
| [susceptibility of clinically isolated strains to aztreonam]. | in vitro antibacterial activities of aztreonam (azt) and cephems against clinically isolated 334 strains were investigated. the results obtained in the study are summarized as follows: 1. azt showed excellent antibacterial activities against clinically isolated 334 strains. 2. azt showed potent activities against escherichia coli, klebsiella pneumoniae, proteus spp., enterobacter aerogenes and citrobacter freundii. 3. antibacterial activities of azt were superior against enterobacter cloacae, se ... | 1988 | 3145344 |
| [susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates]. | susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ... | 1988 | 3163383 |
| rna polymerase as a repressor of transcription in the hut(p) region of mutant klebsiella aerogenes histidine utilization operons. | mutants of klebsiella aerogenes able to express the hutuh operon in the absence of positive effectors were isolated and characterized. these mutations improve the hutuh promoter (puh) by changing the -10 region to match the consensus sequence more closely. these mutations also affect another, oppositely oriented promoter in this region, pc. although the mutations lie far outside pc, they cause pc to be inactive, apparently because binding of rna polymerase to the puh promoter blocks the overlapp ... | 1988 | 3170491 |
| resistance studies with ofloxacin. | the selection of ofloxacin-resistant mutants from susceptible wild-type bacterial populations was investigated by three methods. resistant mutants selected from populations of escherichia coli (nctc 10418) and staphylococcus aureus (nctc 6571) by single-step passage at either four or eight times the mic occurred at a frequency of less than 1 x 10(-10). ofloxacin-resistant mutants of pseudomonas aeruginosa (nctc 10662), selected at four times the mic, occurred with a 100-fold greater frequency at ... | 1988 | 3182460 |
| detection of carbon-phosphorus lyase activity in cell free extracts of enterobacter aerogenes. | the bacterium enterobacter aerogenes could grow on a medium containing alkylphosphonic acid as a phosphorus source. the extracts prepared from the cells grown on phosphonoacetic acid as a sole source of phosphorus showed an activity of carbon-phosphorus lyase and hydrolyzed methyl-phosphonic acid, phosphonoacetic acid and phenylphosphonic acid with a liberation of inorganic phosphates. | 1988 | 3196331 |
| [clinical laboratory approach for estimating effective administrative dose of cefuzonam. evaluation of disc susceptibility test and its interpretation system]. | in vitro activities of cefuzonam (czon) against 273 clinical isolates were studied through the evaluation of mic's and the results of disc susceptibility test. the mic's were determined using the agar dilution method at an inoculum level of 10(6)cfu/ml. the mic80's of czon against streptococcus pneumoniae, escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, haemophilus influenzae and citrobacter spp. were less than 0.20 microgram/ml. the mic80's against serratia marcesc ... | 1988 | 3204654 |
| role of protozoa in microbial acclimation for mineralization of organic chemicals in sewage. | the role of protozoa in affecting the length of the acclimation period for the mineralization of organic compounds in sewage was investigated. the acclimation periods for the mineralization of 2 ng of p-nitrophenol, 100 ng of 2,4-dichlorophenoxyacetic acid, and 100 ng of 2,4-dichlorophenol per milliliter were markedly shortened or eliminated when the indigenous protozoa were inhibited with cycloheximide and nystatin. the extent of mineralization of the test compounds decreased when the protozoa ... | 1988 | 3208203 |
| [clinical laboratory approach for estimating effective administrative dose of sulbactam/cefoperazone]. | sulbactam/cefoperazone (sbt/cpz) is a preparation containing cpz and sbt, an inhibitor of beta-lactamases, at the ratio 1:1. the reliability of the sbt/cpz disc susceptibility test in estimating approximate values of mics and the utilization of the test in the evaluation of proper administrative doses were studied using 365 strains of clinical isolates. the antimicrobial activity of sbt/cpz was stronger than that of cpz alone. this increase in the antimicrobial activity due to the addition of sb ... | 1988 | 3210296 |
| [antibacterial activities of monobactams against fresh clinical isolates]. | antibacterial activities of monobactam antibiotics (carumonam (crmn) and aztreonam (azt] against gram-negative bacilli isolated from inpatients in the latter half of 1987 were investigated using penicillin (pc: piperacillin (pipc], cephems (ceps: ceftazidime (caz), cefotaxime (ctx), latamoxef (lmox), cefsulodin (cfs], carbapenem (imipenem (ipm] and pyridonecarboxylic acids (norfloxacin (nflx) and ofloxacin (oflx] as reference antibiotics. a total of 400 strains of 13 species, i.e. escherichia co ... | 1988 | 3210297 |
| [clinical laboratory approach in estimating the effective dosage of cefamandole]. | reliability of the cefamandole (cmd) disc susceptibility test in estimating approximate values of mics was studied using various clinical isolates totaling 246 strains with showa discs (8 mm diameter containing 30 micrograms of cmd). clinical significance of a 4 category system for the interpretation of the cmd disc tests, which is normally used in japan, was also evaluated to determine whether this system would be suitable or not for the evaluation of a proper dose of administration. the result ... | 1988 | 3221428 |
| [microbiological characteristics and detection of capsular forms of bacteria of the intestinal group in confectionery produced at the candy-chocolate factories]. | five types of confectionery and its semifinished products were investigated for contamination with klebsiella, mesophilic aerobic and elective anaerobic, coliform bacteria, e. coli, etc. after a long-term storage. e. coli and st. aureus were not detected after inoculation on 1 g of the product; mold fungi were identified only in singular samples, their level did not exceed 20 cfu/g; the level of mesophilic aerobic and elective anaerobic bacteria varied from several hundreds to 3000-5500 cfu/g; c ... | 1988 | 3232341 |
| in vitro susceptibility of bacteria to a ticarcillin-clavulanic acid combination. | in vitro testing of bacterial susceptibility to a combination of ticarcillin and clavulanic acid was done, using 406 aerobic gram-positive and gram-negative isolates (considered to be pathogens) cultured from equine and small animal specimens. a microdilution broth technique of susceptibility testing was performed, using trays with wells containing a range of doubling concentrations of dehydrated ticarcillin (range, 0.50 to 128 micrograms/ml) with fixed concentration of clavulanic acid (4 microg ... | 1988 | 3239838 |
| activity of cefixime (fk 027) for resistant gram-negative bacilli. | cefixime is an orally absorbable cephalosporin with an extended spectrum of in vitro activity for gram-negative bacteria especially members of the enterobacteriaceae. gram-negative rod isolates collected over a three month period were tested against cefixime. greater than 99% of escherichia coli isolates was susceptible to cefixime including those resistant to ampicillin. similarly isolates of klebsiella, proteus and providencia were also exquisitely susceptible to cefixime as were 91% of entero ... | 1988 | 3243091 |
| antibacterial activities of cefpodoxime, cefixime, and ceftriaxone. | cefpodoxime, cefixime, and ceftriaxone inhibited branhamella catarrhalis at less than or equal to 1 microgram/ml, beta-hemolytic streptococci at less than or equal to 0.25 microgram/ml, neisseria meningitidis at less than or equal to 0.06 microgram/ml, and haemophilus influenzae (other than beta-lactamase-negative, ampicillin-resistant isolates) at less than or equal to 0.12 microgram/ml. the mics for 50% of isolates of the family enterobacteriaceae other than citrobacter freundii, enterobacter ... | 1988 | 3245701 |
| clinical efficacy of carumonam. | carumonam is a new n-sulfo-beta-lactam antibiotic active against aerobic gram-negative bacteria. an open study was carried out to evaluate the efficacy, safety and tolerance of carumonam with either 1 g t.i.d. (group a) or 2 g t.i.d. (group b) in bacterial septicaemia or severe sepsis. a total of 24 patients (14 men and 10 women) were included in the study, their ages ranged from 48-87 years (mean age 59). eighteen patients were treated for bacteraemia, three for bronchopneumonia, two for urinar ... | 1988 | 3246211 |
| in vitro activity of an oral iminomethoxy aminothiazolyl cephalosporin, r-3746. | the in vitro activity of r-3746, an iminomethoxy aminothiazolyl cephalosporin with a ch2och3 moiety at position 3, was compared with those of other antibiotics. r-3746 inhibited the majority of hemolytic streptococci (groups a, b, c, f, and g) and streptococcus pneumoniae at less than 0.06 micrograms/ml, which was comparable to the activity of amoxicillin, 2- to 8-fold more active than cefixime, and 16- to 64-fold more active than cefaclor and cephalexin. ninety percent of beta-lactamase-produci ... | 1988 | 3260766 |
| eari, a restriction endonuclease from enterobacter aerogenes which recognizes 5'ctcttc3'. | 1988 | 3263622 | |
| nucleotide sequence and expression of the enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast. | the nucleotide sequence of a 1.4-kilobase dna fragment containing the alpha-acetolactate decarboxylase gene of enterobacter aerogenes was determined. the sequence contains an entire protein-coding region of 780 nucleotides which encodes an alpha-acetolactate decarboxylase of 260 amino acids. the dna sequence coding for alpha-acetolactate decarboxylase was placed under the control of the alcohol dehydrogenase i promoter of the yeast saccharomyces cerevisiae in a plasmid capable of autonomous repl ... | 1988 | 3278689 |
| tigemonam, an oral monobactam. | tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ... | 1988 | 3279906 |
| the artificial heart: infection-related morbidity and its effect on transplantation. | between october, 1985, and september, 1987, a total of 195 patients received cardiac allografts and 15 candidates required mechanical support with the jarvik-7 total artificial heart. seven of the 15 died within 60 days of total artificial heart implant. there have been no late deaths, and survivors are unrestricted. six of 7 deaths were related to infection (mediastinitis, 5; pneumonia and sepsis, 1), and the remaining 1 was due to failure of the transplanted heart. respiratory tract infection ... | 1988 | 3281615 |
| analysis by the reductive-cleavage method of linkage positions in a polysaccharide containing 4-linked d-glucopyranosyluronic residues. | the fate of 4-linked d-glucopyranosyluronic residues under reductive-cleavage conditions was investigated by using the klebsiella aerogenes type 54 strain a3 capsular polysaccharide. treatment of the fully methylated polysaccharide with triethylsilane and trimethylsilyl trifluoromethanesulfonate in dichloromethane, followed by in situ acetylation, yielded 1,5-anhydro-2,3,4,6-tetra-o-methyl-d-glucitol, 3,4-di-o-acetyl-1,5-anhydro-2,6-di-o-methyl-d-glucitol, and 3-o-acetyl-1,5-anhydro-2,4-di-o-met ... | 1988 | 3288343 |
| interactions between antibacterial agents of the quinolone group and nitrofurantoin. | plate diffusion tests showed that nitrofurantoin antagonized the activity of nalidixic acid, norfloxacin, ciprofloxacin and enoxacin against many gram-negative bacilli, including all proteus mirabilis and klebsiella aerogenes strains tested. no interaction was exhibited when nitrofurantoin and the newer quinolones were tested against pseudomonas aeruginosa, staphylococci or streptococci. antagonism was not reliably demonstrated in chessboard titrations, especially with the newer quinolones. cont ... | 1988 | 3356622 |
| cloning and nucleotide sequence of the isoamylase gene from pseudomonas amyloderamosa sb-15. | the gene (iam) coding for isoamylase (glycogen 6-glucanohydrolase) of pseudomonas amyloderamosa sb-15 was cloned. its nucleotide sequence contained an open reading frame of 2313 nucleotides (771 amino acids) encoding a precursor of secreted isoamylase. the precursor contained a signal peptide of 26 amino acid residues at its amino terminus and three regions homologous with those conserved in alpha-amylases (1,4-alpha-d-glucan 4-glucanohydrolase) of species ranging from prokaryotes to eukaryotes. ... | 1988 | 3379068 |
| risk factors for development of sepsis in a hospital outbreak of enterobacter aerogenes. | 1988 | 3408015 | |
| a laboratory model for testing agents for hygienic hand disinfection: handwashing and chlorhexidine for the removal of klebsiella. | a model for the laboratory assessment of hygienic hand disinfection is described which closely reproduces handwashing in a ward setting. the model uses a clinically relevant test organism and takes into account the natural antibacterial action of the skin. it also provides a means of measuring any residual antibacterial action after hand disinfection. using an epidemic multiply-resistant strain of klebsiella aerogenes k21, 0.5% chlorhexidine in iso-propyl alcohol yielded sterile post-disinfectio ... | 1988 | 2904456 |
| effect of macromolecular composition of microorganisms on the thermodynamic description of their growth. | most models describing bacterial growth, including the original mosaic non-equilibrium thermodynamic (mnet) description, do not take into account that the macromolecular composition of the cells varies with growth rate. the mnet description of bacterial growth is extended to account for such a variation in macromolecular composition of the cells in order to make the mnet description more generally applicable. klebsiella aerogenes nctc 418 was cultured in a chemostat under glucose- or ammonia-lim ... | 1988 | 2461738 |
| lipid a precursor from pseudomonas aeruginosa is completely acylated prior to addition of 3-deoxy-d-manno-octulosonate. | inhibition of lipopolysaccharide (lps) synthesis in pseudomonas aeruginosa at the stage of incorporation of 3-deoxy-d-manno-octulosonate (kdo) caused accumulation of a lipid a precursor which contained all of the fatty acids present on the lipid a of mature lps. the enzyme ctp:cmp-3-deoxy-d-manno-octulosonate cytidylyltransferase (cmp-kdo synthetase) from p. aeruginosa is inhibited by the kdo analog alpha-c-[1,5-anhydro-8-amino-2,7,8-trideoxy-d-manno-octopyranosyl] carboxylate (i), and i is effe ... | 1988 | 2833499 |
| bidirectional promoter in the hut(p) region of the histidine utilization (hut) operons from klebsiella aerogenes. | the hut(p) region (i.e., the region responsible for regulation of hutuh expression) of the klebsiella aerogenes histidine utilization (hut) operons contains a bidirectional promoter. one transcript from this promoter encodes the hutuh operon; the role of the oppositely directed transcript is unknown, although it appears to be involved in regulating hutuh expression (a.j. nieuwkoop, s.a. boylan, and r.a. bender, j. bacteriol. 159:934-939, 1984). a 247-base-pair (bp) fragment containing hut(p) car ... | 1988 | 2834335 |
| contact alters camp metabolism in aggregation-competent dictyostelium amoebae. | camp and cell-cell contact are involved in the coordination of differentiation and morphogenesis in dictyostelium discoideum. the experiments described in this paper establish a relationship between camp and cell-cell contact. contact between enterobacter aerogenes and aggregation-competent dictyostelium amoebae and contact between dictyostelium amoebae themselves results in the transient secretion of camp and an alteration in the amount of camp secreted in response to subsequent stimulation by ... | 1988 | 2854020 |
| plasmid-mediated conjugative transfer of klebsiella sp. rcs genesable to induce colanic acid capsular polysaccharide biosynthesis in escherichia coli. | regulation of capsular biosynthesis (rcs) genes, encoding the ability to induce the production of a colanic acid polysaccharide capsule, were transferred to escherichia coli by conjugation with klebsiella pneumoniae (aerogenes) of capsular serotype k36. transfer was mediated by a 58.4-mda conjugative plasmid of incompatibility group incm, which carried a copy of tn7 (specifying resistance to trimethoprim and streptomycin) together with determinants for several further resistances. this plasmid d ... | 1988 | 2856173 |
| studies on lysine:n6-hydroxylation by cell-free systems of aerobacter aerogenes 62-1. | electron microscopic examination has revealed the vesicular nature of the membrane component, of the cell-free system of aerobacter aerogenes 62-1, which catalyses lysine: n6-hydroxylation. regardless of the orientation of the vesicles, n-hydroxylation process is still stimulated by pyruvate. both pyruvate oxidation and lysine: n6-hydroxylation were inhibited by protonophores and gramicidin s. | 1989 | 2493814 |
| evolution of chemotactic-signal transducers in enteric bacteria. | the methyl-accepting chemotactic-signal transducers of the enteric bacteria are transmembrane proteins that consist of a periplasmic receptor domain and a cytoplasmic signaling domain. to study their evolution, transducer genes from enterobacter aerogenes and klebsiella pneumoniae were compared with transducer genes from escherichia coli and salmonella typhimurium. there are at least two functional transducer genes in the nonmotile species k. pneumoniae, one of which complements the defect in se ... | 1989 | 2496104 |
| bacterial colonization of intravenous catheter materials in vitro and in vivo. | four different intravenous catheter materials, brands teflon, silastic, vialon, and tecoflex, were evaluated in vitro for bacterial adherence after 2 and 24 hours' incubation in trypticase soy broth and after 2 hours' incubation in nutrient-free phosphate buffer (ph 7.2). the organisms used were pseudomonas aeruginosa, enterobacter aerogenes, escherichia coli, and staphylococcus epidermidis. the significant differences in in vitro adherence of the different bacterial species to the various cathe ... | 1989 | 2500724 |