Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pjp4. | plasmid pjp4 is an 80-kilobase, incp1, broad-host-range conjugative plasmid of alcaligenes eutrophus encoding resistance to mercuric chloride and phenyl mercury acetate and degradation of 2,4-dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid, and 3-chlorobenzoate. by the use of cloning, transposon mutagenesis, and restriction endonuclease analysis, a biophysical and genetic map of pjp4 was generated. | 1985 | 3968031 |
| occurrence and antimicrobial susceptibility of gram-negative nonfermentative bacilli in cystic fibrosis patients. | isolation of nonfermentative gram-negative bacilli (other than pseudomonas aeruginosa) from respiratory tract cultures of cystic fibrosis (cf) patients has increased in recent years. species recovered include pseudomonas cepacia, p. maltophilia, p. fluorescens/putida, p. alcaligenes, p. pseudoalcaligenes, p. stutzeri, acinetobacter spp., achromobacter xylosoxidans, flavobacterium spp., and cdc groups ive and ve. although colonization with most of these organisms is sporadic, p. cepacia (and to a ... | 1985 | 3979021 |
| [biochemical quality assessment of broiler production using a hydrogen bacteria biomass in the diet]. | the authors studied the biochemical composition of the meat of broilers, eggs, liver and muscles of laying hens of 3 generations on a 5, 10, 25, 50 and 100% (broilers), 10 and 20% (laying hens) replacement of the animal protein quota in the diet by protein obtained from the hydrogen bacteria alcaligenes eutrophus l-1. no deterioration of the quality of the produce was found from the standpoint of the main biochemical parameters. | 1985 | 3984290 |
| antibiotic aerosolization: the effect on experimentally induced alcaligenes rhinotracheitis in turkeys. | oxytetracycline hydrochloride was delivered by aerosol twice daily for 3 days to uninfected turkeys and to turkeys experimentally inoculated with alcaligenes faecalis. the clinical, microbiological, and histological changes in the upper respiratory tracts were studied. no lesions were observed in the tracheas of uninoculated poults exposed to the aerosol. in experimentally infected poults, clinical signs included ocular and nasal discharges and open-mouthed breathing. histologic lesions included ... | 1985 | 3985873 |
| respiratory disease (rhinotracheitis) in turkeys in brittany, france, 1981-1982. i. field observations and serology. | during the summer of 1981, a respiratory disease epidemic occurred in turkeys in brittany, france. since this initial epizootic, which lasted through fall, epizootic waves similar to the initial one have occurred at approximately 6-month intervals, with smaller peaks at 2-month intervals. the epidemiology, clinical signs, and postmortem findings were highly suggestive of an epizootic of chlamydiosis. serological tests for chlamydia, paramyxoviruses, avian influenza, adenovirus 127, mycoplasma, a ... | 1985 | 3985881 |
| alcaligenes faecalis infection in turkeys: effects on serum corticosterone and serum chemistry. | the serum levels of corticosterone, total calcium, inorganic phosphorus, protein, sodium, and potassium were determined for turkey poults from 1 to 42 days of age. corticosterone levels were higher at days 10, 14, 21, and 28 in alcaligenes faecalis-infected turkeys than in uninfected poults. the corticosterone responses in this study indicate that a. faecalis infection stimulates adrenal cortical function even in the very young poult, which ordinarily does not show adrenal cortical responsivenes ... | 1985 | 3985885 |
| aspartate beta-decarboxylase from alcaligenes faecalis: carbon-13 kinetic isotope effect and deuterium exchange experiments. | we have measured the 13c kinetic isotope effect at ph 4.0, 5.0, 6.0, and 6.5 and in d2o at pd 5.0 and the rate of d-h exchange of the alpha and beta protons of aspartic acid in d2o at pd 5.0 for the reaction catalyzed by the enzyme aspartate beta-decarboxylase from alcaligenes faecalis. the 13c kinetic isotope effect, with a value of 1.0099 +/- 0.0002 at ph 5.0, is less than the intrinsic isotope effect for the decarboxylation step, indicating that the decarboxylation step is not entirely rate l ... | 1985 | 4005219 |
| molecular and immunological comparison of membrane-bound, h2-oxidizing hydrogenases of bradyrhizobium japonicum, alcaligenes eutrophus, alcaligenes latus, and azotobacter vinelandii. | the membrane-bound hydrogenases of bradyrhizobium japonicum, alcaligenes eutrophus, alcaligenes latus, and azotobacter vinelandii were purified extensively and compared. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of each hydrogenase revealed two prominent protein bands, one near 60 kilodaltons and the other near 30 kilodaltons. the migration distances during nondenaturing polyacrylamide gel electrophoresis were similar for all except a. vinelandii hydrogenase, which migrated furth ... | 1985 | 4008438 |
| [gram-negative nonfermenting bacteria in respiratory tract infections]. | 1985 | 4010380 | |
| [nature of factors stimulating cobalamin production by achromobacter cobalamini]. | the object of this work was to study the nature of factors contained in molasses and maize extract and stimulating cobalaminogenesis in achromobacter cobalamini. the activity of substrate fractions was analyzed to show that the stimulating substance was precipitated on the cation exchanger and eluted from it with hcl. the factor was found to be an organic nitrogen base readily soluble in water and ethanol but insoluble in ether, chloroform and methanol. it was stable upon heating in concentrated ... | 1985 | 4010546 |
| single and twinned crystals of ribulose-1,5-bisphosphate carboxylase-oxygenase from alcaligenes eutrophus. | ribulose-1,5-bisphosphate carboxylase-oxygenase (l8s8) from alcaligenes eutrophus has been crystallized by equilibrium vapor diffusion techniques with ammonium sulfate as precipitant. crystals thus obtained either as the ternary complex with co2 and mg2+ or as the quaternary complex with co2, mg2+, and 2-carboxyarabinitol 1,5-bisphosphate, a transition state analogue, diffract at least to 2.8-a resolution. both are essentially isomorphous to each other, having orthorhombic space group c222(1) wi ... | 1985 | 4030770 |
| [amino acid composition of white poultry meat contaminated with psychrophilic microorganisms]. | studied was the effect of psychrophilic organisms of the acinetobacter, aeromonas, alcaligenes, and flavobacter genera on the amino acid composition of white poultry meat stored at 0 degrees to 4 degrees c in the course of 48 hours, and at 18 degrees c in the course of 6 to 12 months. the quantitative determination of individual amino acids was carried out by means of an automatic amino acid analyzer, hitachi type, after the method of speckman, stein, and moore. no changes were established in th ... | 1985 | 4035999 |
| neonatal meningitis caused by achromobacter xylosoxidans. | the clinical and bacteriological findings in a case of neonatal meningitis caused by achromobacter xylosoxidans are presented. this appears to be only the second report of meningitis caused by this species. | 1985 | 4044804 |
| [microflora in swine slurry as a parameter in determining the efficiency of deodorizing treatment. i. continuous and discontinuous aeration treatment]. | liquid manure from the pig, fresh and stored, was treated by continuous and discontinuous aeration. the purpose was to achieve a biologically stabilized and deodorized organic material. the samples which were aerated intermittently had good decomposition rates for nitrogen compounds and even so good deodorization effects. in spite of a minimum of dissolved oxygen of appr. 0.2 mg/l, good deodorization could be achieved by intermittent aeration for 23 days. simultaneous processes of nitrification ... | 1985 | 4050182 |
| the influence of milk and milk components on the attachment of bacteria to farm dairy equipment surfaces. | glass, rubber and stainless steel surfaces were exposed to various types of bacteria in the presence of milk and a number of milk components under both static and agitated incubation conditions. numbers of bacteria attaching were enumerated by epifluorescence microscopy. results were affected by the different bacterial types, the nature of the attachment surface and the substances in which the bacteria were suspended with a moraxella-like species, stainless steel and lactose and non-casein prote ... | 1985 | 4066551 |
| the role of s-adenosylhomocysteine in the biological utilization of s-adenosylmethionine. | 1985 | 4070312 | |
| further characterization of the agent causing coryza in turkeys. | a total of 128 isolates of alcaligenes faecalis, from the respiratory tract of turkeys and chickens, were identified and divided into two types designated type i and type ii. type i isolates were pathogenic in poults, hemagglutinated guinea pig red blood cells (rbcs), and did not grow on minimal essential medium (mem) agar, and most did not grow in 6.5% nacl broth. type ii isolates were nonpathogenic and nonhemagglutinating and grew on mem agar, and most grew in 6.5% nacl broth. hemagglutination ... | 1985 | 4074238 |
| immune response of poults following intranasal inoculation with artvax vaccine and a formalin-inactivated bordetella avium bacterin. | poults 3 weeks and older developed temporary tracheal resistance to intranasal challenge following inoculation of either artvax vaccine or formalin-inactivated bordetella avium bacterin by the intranasal and eyedrop routes. resistance usually persisted for 3-4 weeks after b. avium challenge. however, with constant exposure to infected controls, the vaccinated birds eventually developed tracheal infection. day-old poults did not respond to either the artvax or the bacterin and were completely sus ... | 1985 | 4074243 |
| holding effects on coliform enumeration in drinking water samples. | standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ... | 1985 | 4083877 |
| effects of freezing on purified nitrite reductase from a denitrifier, alcaligenes sp. ncib 11015. | the effects of freezing on alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, ec 1.7.2.1] dissolved in sodium phosphate (ph 7.2) were investigated. the nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. the final freezing temperature influenced the enzyme activation, maximal activation being observed at around -20 degrees c. all the enzymatic activities that the nitrite reductase is k ... | 1985 | 4086480 |
| the primary structures of pseudomonas am1 amicyanin and pseudoazurin. two new sequence classes of blue copper proteins. | the amino acid sequences of two blue copper proteins from the pink facultative methylotroph pseudomonas am1 (n.c.i.b. 9133) were determined. they each consist of a single polypeptide chain and bind one copper atom. amicyanin contains 99 and pseudoazurin 123 residues. copper-binding sites, consisting of the side chains of two histidine, one cysteine and one methionine residues, can be recognized in each protein by analogy with azurin and plastocyanin, but the spacings of the ligand residues are d ... | 1985 | 4091802 |
| difference spectroscopy with respiratory membranes of an h2-oxidizing microorganism layered between two gas-permeable, plastic foils: a procedure that allows measurements in the ultraviolet range. | a method for recording redox difference spectra of respiratory membranes which minimizes light scattering and results in increased sensitivity and spectral range when compared to the traditional technique of recording spectra of respiratory particles in suspension is described. membranes were sandwiched between two gas-permeable, plastic foils, placed in a sealed cuvette, and gassed with h2 as reductant or o2 as oxidant. the membranes of the h2-oxidizing microorganism (alcaligenes eutrophus h16) ... | 1985 | 4096385 |
| transposon mutagenesis and cloning analysis of the pathways for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in alcaligenes eutrophus jmp134(pjp4). | plasmid pjp4 permits its host bacterium, strain jmp134, to degrade and utilize as sole sources of carbon and energy 3-chlorobenzoate and 2,4-dichlorophenoxyacetic acid (r. h. don and j. m. pemberton, j. bacteriol. 145:681-686, 1981). mutagenesis of pjp4 by transposons tn5 and tn1771 enabled localization of five genes for enzymes involved in these catabolic pathways. four of the genes, tfdb, tfdc, tfdd, and tfde, encoded 2,4-dichlorophenol hydroxylase, dichlorocatechol 1,2-dioxygenase, chloromuco ... | 1985 | 2981813 |
| a comparative study of the resonance raman spectra of bacterial cytochromes. | resonance raman spectra were obtained for two newly isolated bacterial cytochromes, alcaligenes faecalis (atcc 8750) c554 and alcaligenes faecalis c556. their spectra were compared with those of mammalian cytochrome c and two other bacterial cytochromes, paracoccus denitrificans c550 and pseudomonas aeruginosa c551. the positions of the raman bands indicated that, while al. c554 and al. c556 were c-type cytochromes with two thioether linkages, several common features found in their raman spectra ... | 1985 | 2982314 |
| a small mobilizable incp group plasmid vector packageable into bacteriophage lambda capsids in vitro. | a mobilizable cosmid derivative of an incp group plasmid was constructed by cloning the orit region of rk2, a wide host-range plasmid, and the minimal dna sequence of bacteriophage lambda required for efficient packaging in vitro. this cosmid is 13 kb in size and has unique restriction sites for ecori, xhoi, hindiii, and sali. the xhoi and hindiii sites are within the kanamycin-resistance gene and the sali site is in the tetracycline-resistance gene. this plasmid was mobilizable from an escheric ... | 1985 | 2986189 |
| hydrogen peroxide as a tumoricidal mediator of murine polymorphonuclear leukocytes induced by a linear beta-1,3-d-glucan and some other immunomodulators. | polymorphonuclear leukocytes (pmn) of mice can destroy tumor cells effectively in vitro in the presence of antitumor polysaccharide, linear beta-1, 3-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 (tak), and some other immunomodulators. in the present study, we investigated the mechanism of the tumoricidal activity of pmn induced by these immunomodulators and especially tak. the tak-induced pmn cytotoxicity was concluded to involve hydrogen peroxide from the following results: (a) t ... | 1985 | 2990672 |
| plasmid-mediated mineralization of 4-chlorobiphenyl. | strains of alcaligenes and acinetobacter spp. were isolated from a mixed culture already proven to be proficient at complete mineralization of monohalogenated biphenyls. these strains were shown to harbor a 35 x 10(6)-dalton plasmid mediating a complete pathway for 4-chlorobiphenyl (4cb) oxidation. subsequent plasmid curing of these bacteria resulted in the abolishment of the 4cb mineralization phenotype and loss of even early 4cb metabolism by acinetobacter spp. reestablishment of the alcaligen ... | 1985 | 2993249 |
| identification of heme axial ligands of cytochrome c' from alcaligenes sp. n.c.i.b. 11015. | the spectral properties of both ferric and ferrous cytochromes c' from alcaligenes sp. n.c.i.b. 11015 are reported. the epr spectra at 77 k and the electronic, resonance raman, cd and mcd spectra at room temperature have been compared with those of the other cytochromes c' and various hemoproteins. in the ferrous form, all the spectral results at physiological ph strongly indicated that the heme iron(ii) is in a high-spin state. in the ferric form, the epr and electronic absorption spectra were ... | 1985 | 2996606 |
| chromosomal and plasmid locations for phosphoribulokinase genes in alcaligenes eutrophus. | genes coding for phosphoribulokinase (prk), a key enzyme of the calvin cycle, were localized in the genome of the chemoautotroph alcaligenes eutrophus. the nh2-terminal sequence of the prk subunit was determined. with a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed prk genes to be located on both the chromosome and the megaplasmid phg1 of a. eutrophus h16. | 1985 | 2997141 |
| isolation of novel microbial 3 alpha-, 3 beta-, and 17 beta-hydroxysteroid dehydrogenases. purification, characterization, and analytical applications of a 17 beta-hydroxysteroid dehydrogenase from an alcaligenes sp. | by selecting for growth on testosterone or estradiol-17 beta as the only source of organic carbon, we have isolated a number of soil microorganisms which contain highly active and novel, inducible, nad-linked 3 alpha-, 3 beta-, and 17 beta-hydroxysteroid dehydrogenases. such enzymes are suitable for the microanalysis of steroids and of steroid-transforming enzymes, as well as for performing stereoselective oxidations and reductions of steroids. of particular interest among these organisms is a n ... | 1985 | 2997177 |
| taurine-glutamate transaminase. | 1985 | 3003495 | |
| induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-d-glucan and other immunomodulators in murine cells. | the cytotoxic activity of polymorphonuclear leukocytes (pmn) against tumor cells induced in vitro by antitumor immunomodulators was examined by a 51cr release cytotoxicity assay. among 28 immunomodulators and other agents thus far tested, only beta-1,3-glucan from alcaligenes faecalis var. myxogenes ifo 13140, bacillus calmette-guérin, propionibacterium acnes, zymosan a, and nocardia cell wall skeleton were found to cause induction. the cytotoxic activity of pmn with the beta-1,3-glucan was very ... | 1985 | 3156669 |
| comparative in-vitro activity of ciprofloxacin against non-fermenters. | the in-vitro activity of ciprofloxacin, a quinolone-carboxylic acid derivative, was compared with those of carbenicillin, azlocillin, cefsulodin, ceftazidime, tobramycin and amikacin against 187 non-fermenters. only one of the 131 strains of pseudomonas spp. was not inhibited by 1 mg/l of ciprofloxacin, while these isolates appeared highly resistant to carbenicillin, azlocillin and cefsulodin. ciprofloxacin was also the best agent against flavobacterium, alcaligenes faecalis and acinetobacter ca ... | 1985 | 3159710 |
| efficacy of a commercial turkey coryza vaccine (art-vax) in turkey poults. | four laboratory experiments were designed to study the efficacy of the only available commercial vaccine for turkey coryza, art-vax. poults were vaccinated either once or twice at different ages and challenged with pathogenic alcaligenes faecalis. in another study, commercial turkeys vaccinated at 1 and 12 days of age on a commercial farm were brought to the laboratory for challenge with pathogenic a. faecalis. both the laboratory- and field-vaccinated poults were given the manufacturer's recomm ... | 1985 | 3833219 |
| antibiotic susceptibilities of achromobacter xylosoxidans. | 1985 | 3834609 | |
| isolation of bordetella avium from poultry. | four australian isolates of gram-negative nonfermentative bacteria obtained from poultry were compared with reference strains of bordetella avium, bordetella bronchiseptica and alcaligenes faecalis. the australian isolates were identified as bordetella avium. a routine procedure for the identification of this recently recognised poultry pathogen is described. | 1985 | 3834901 |
| genes specifying degradation of 3-chlorobenzoic acid in plasmids pac27 and pjp4. | all of the structural genes for 3-chlorobenzoate degradation are clustered in a 4.2-kilobase (kb) region of plasmid pac25 (or pac27) in pseudomonas putida. an approximate 10-kb dna segment containing three structural genes for chlorocatechol metabolism present on plasmid pjp4 in alcaligenes eutrophus shows homology with the above 4.2-kb region of pac27. in spite of the detectable sequence homology in the structural genes present on both plasmids, the regulation of their expression seems quite di ... | 1985 | 3856842 |
| purification and properties of inducible penicillin beta-lactamase isolated from alcaligenes faecalis. | an inducible penicillin beta-lactamase was purified from a strain of alcaligenes faecalis resistant to beta-lactam antibiotics. the purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis, and its molecular weight was 29,000 based on sodium dodecyl sulfate-acrylamide gel electrophoresis. its isoelectric point was 5.9. the enzyme more rapidly hydrolyzed penicillins, such as penicillin g, ampicillin, carbenicillin, piperacillin, and cloxacillin, than it hydroly ... | 1985 | 3873902 |
| purification and properties of a beta-lactamase from alcaligenes dentrificans subsp. xylosoxydans. | a penicillin beta-lactamase was purified from a strain of alcaligenes dentrificans subsp. xylosoxydans resistant to beta-lactam antibiotics. the purified enzyme preparation gave a single protein band on polyacrylamide gel electrophoresis, and its molecular weight was 18,000 from sodium dodecylsulphate-acrylamide gel electrophoresis and gel filtration. its isoelectric point was 9.8, the optimal ph was 8.5 and the optimal temperature was 35 degrees c. the enzyme hydrolyzed penicillin g and ampicil ... | 1985 | 3877040 |
| identification of gram-negative bacilli using the autobac idx. | the autobac idx is a new system for the rapid identification of clinically significant members of the enterobacteriaceae and aeromonas, acinetobacter, alcaligenes, flavobacterium, moraxella, and pseudomonas species. the use of 18 differentially inhibitory compounds such as dyes and antibiotics along with a computerized algorithm based on a multivariate analysis provides the basis for the identification of 30 different groups of gram-negative bacilli. required preliminary tests include observatio ... | 1985 | 3881212 |
| alcaligenes eutrophus ch34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals. | alcaligenes eutrophus strain ch34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with a. eutrophus strain h16 the ability to grow lithoautotrophically on molecular hydrogen, to form a cytoplasmic nad-reducing and a membrane-bound hydrogenase, and most metabolic attributes; however, it does not grow on fructose. strain ch34 contains two plasmids, pmol28 (163 kilobases) specifying nickel, mercury, and cobalt resistance and pmol30 (238 kilobases) specifying z ... | 1985 | 3884593 |
| denitrification by alcaligenes eutrophus is plasmid dependent. | curing of the hydrogenase-specifying megaplasmid phg indigenous to strains of the facultative lithoautotrophic bacterium alcaligenes eutrophus was correlated with a loss of denitrifying ability (nitd). the retransfer of plasmid phg1 reconstituted the nitd phenotype. plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic conditions. | 1985 | 3886640 |
| the activity of ceftazidime compared with those of aztreonam, newer cephalosporins and sch 29482 against nonfermentative gram-negative bacilli. | the activity of ceftazidime was compared with those of aztreonam, newer cephalosporins and sch 29482 against nonfermentative gram-negative bacilli. ceftazidime was consistently more active (mic less than or equal to 8 micrograms) against the nonfermenters. only flavobacterium odoratum, f. spp., pseudomonas alcaligenes, p. maltophilia and p. stutzeri demonstrated substantial resistance (mic90 greater than or equal to 64 micrograms) to ceftazidime. sch 29482 and ceftriaxone also exhibited good act ... | 1985 | 3888543 |
| evaluation of api 20 ne in routine diagnostics of nonfermenting gram-negative rod-shaped bacteria. | 292 strains of non-fermenting gram-negative rod-shaped bacteria were determined by standard methods and tested in a new commercial microidentification-system, the api 20 ne (api biomérieux). a total of 282 (= 96,6%) strains were identified conformably; 130 (= 46,1%) after 24 h and 77 (= 27,3%) within 48 h. in 75 (= 26,6%) cases different conventional additional tests for determination to species level were necessary. the reasons for divergent results of 10 strains are discussed. the new microide ... | 1985 | 3890425 |
| an alcaligenes faecalis isolate from turkeys: pathogenicity in selected avian and mammalian species. | an alcaligenes faecalis isolate of known pathogenicity for turkeys was examined for adherence and cytotoxicity in tracheal organ cultures of turkeys, chickens, japanese quail, guinea pigs, hamsters, and mice, and for colonization and pathogenicity in these 6 species. adherence and colonization were detected by fluorescent antibody staining. infected and noninfected tracheal rings were examined by phase-contrast microscopy for cytotoxicity (ciliostasis, blebing of the cell membrane, and sloughing ... | 1985 | 3890634 |
| [dissociative model of the thermoinactivation of nad-dependent hydrogenase]. | 1985 | 3893960 | |
| mathematical model for determining the effects of intracytoplasmic inclusions on volume and density of microorganisms. | procaryotic microorganisms accumulate several polymers in the form of intracellular inclusions as a strategy to increase survival in a changing environment. such inclusions avoid osmotic pressure increases by tightly packaging certain macromolecules into the inclusion. in the present paper, a model describing changes in volume and density of the microbial cell as a function of the weight of the macromolecule forming the inclusion is derived from simple theoretical principles. the model is then t ... | 1985 | 3902798 |
| rhizobium japonicum hydrogenase: purification to homogeneity from soybean nodules, and molecular characterization. | rhizobium japonicum hydrogenase was purified to homogeneity from soybean root nodules by four column chromatography steps after solubilization from membranes by treatment with a nonionic detergent. the specific activity was from 40 to 65 mumol h2 oxidized min-1 mg protein-1 and was increased 450-fold relative to that in bacteroids. the yield of activity was from 7 to 12%. the molecular weight of the native enzyme was 104,000 as determined by sucrose density gradient centrifugation. electrophores ... | 1985 | 3919648 |
| anti-pseudomonal activity of hr 810. | the anti-pseudomonal activity of hr 810, a new 2-aminothiazolyl cephalosporin, was compared to that of carbenicillin, azlocillin and cefsulodin against 187 non-fermenters. hr 810 was the best agent against pseudomonas aeruginosa, pseudomonas putida, pseudomonas fluorescens and acinetobacter calcoaceticus with an mic50 less than or equal to 4 mg/l and an mic90 less than or equal to 16 mg/l. it was as effective as azlocillin against pseudomonas stutzeri and pseudomonas mendocina, with an mic50 les ... | 1985 | 3922898 |
| [tetracycline resistance controlled by plasmids of pseudomonas aeruginosa with a wide spectrum of bacterial hosts]. | pbs222 and pbs223 plasmids with a wide spectrum of bacterial hosts detected in strains of p. aeruginosa control resistance of the cells of p. aeruginosa ml4262 (pao) and e. coli c600 to 300 and 200 micrograms/ml of oxytetracycline respectively increasing 15 and 20 times their resistance to the antibiotics. the constant of the antibiotic elimination from the cells of p. aeruginosa ml4262 containing pbs223 plasmid is 3.5 times higher than the entry constant, while the constants of the antibiotic e ... | 1985 | 3923917 |
| characterization of aquatic bacteria and cloning of genes specifying partial degradation of 2,4-dichlorophenoxyacetic acid. | water samples from rivers, streams, ponds, and activated sewage were tested for the presence of bacteria which utilize 2,4-dichlorophenoxyacetic acid (2,4-d) as a sole source of carbon. seventy percent of the attempted enrichments yielded pure cultures of 2,4-d-metabolizing bacteria. all but 1 of the 30 isolates were gram-negative rods, all but 2 were motile, and all were nonfermentative and oxidase and catalase positive. nine isolates had dna guanine-plus-cytosine values of 61.1 to 65 mol%. one ... | 1985 | 3923930 |
| thymidine salvage in pseudomonas stutzeri and pseudomonas aeruginosa provided by heterologous expression of escherichia coli thymidine kinase gene. | unlike enteric bacteria, pseudomonas spp. generally lack thymidine phosphorylase and thymidine kinase activities, thus preventing their utilization of exogenous thymine or thymidine and precluding specific radioactive labeling of their dna in vivo. to overcome this limitation, a dna fragment encoding thymidine kinase (ec 2.7.1.21) from escherichia coli was cloned into pkt230, a small, broad-host-range plasmid derived from plasmid rsf1010. from transformed e. coli colonies, the recombinant plasmi ... | 1985 | 3924894 |
| nitric oxide-dependent proton translocation in various denitrifiers. | respiration of no resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. paracoccus denitrificans, rhodopseudomonas sphaeroides subsp. denitrificans, "achromobacter cycloclastes," and rhizobium japonicum gave, respectively, h+/no ratios of 3.65, 4.96, 1.94, and 1.12. antimycin a completely inhibited no-dependent proton translocation in p. denitrificans and severely restricted translocation in the r. sphaeroides strain. proton uptake ... | 1985 | 3928599 |
| [three cases of septicemia caused by glucose non-fermentative gram-negative rods]. | 1985 | 3932537 | |
| formadicins, new monocyclic beta-lactam antibiotics of bacterial origin. i. taxonomy, fermentation and biological activities. | a gram-negative bacterium produces new monocyclic beta-lactam antibiotics with a formylamino substituent, named formadicins a, b, c and d. the producing bacterium was taxonomically characterized and designated as flexibacter alginoliquefaciens sp. nov. yk-49. formadicins have narrow antibacterial spectra. they are highly active against some species of pseudomonas, proteus and alcaligenes. of the four, formadicin c shows the most potent antibacterial activity. several amino acids such as glycine, ... | 1985 | 3934120 |
| a simple kinetic test to distinguish exo-glucosidase and beta-glucosidase activities. | unusual kinetic behaviour was observed in assaying spectrophotometrically for exo-glucanase activity in a beta-glucosidase isolated from a. faecalis using p-nitrophenyl beta-cellobioside as substrate. at high substrate concentrations no phenol was released whereas at low concentrations a rapid release of phenol was detected and this increased in rate with extent of hydrolysis. these results are consistent with a model involving tight binding of the substrate to the enzyme and an initial exo-gluc ... | 1985 | 3936498 |
| alpha-aminoadipate. | 1985 | 3937020 | |
| degradation of poly(3-hydroxybutyrate) by poly(3-hydroxybutyrate) depolymerase from alcaligenes faecalis t1. | the extracellular poly(3-hydroxybutyrate) depolymerase purified from alcaligenes faecalis t1 has two disulfide bonds, one of which appears to be necessary for the full enzyme activity. this depolymerase hydrolyzed not only hydrophobic poly(3-hydroxybutyrate) but also water-soluble trimer and larger oligomers of d-(-)-3-hydroxybutyrate, regardless of their solubilities in water. kinetic analyses with oligomers of various sizes indicated that the substrate cleaving site of the enzyme consisted of ... | 1986 | 3942778 |
| mechanism of alpha-amino-epsilon-caprolactam racemase reaction. | alpha-amino-epsilon-caprolactam racemase catalyzes the exchange of the alpha-hydrogen of the substrate with deuterium during racemization in deuterium oxide. the rate of the hydrogen exchange measured by 1h nmr is lower than that of racemization in deuterium oxide for both the enantiomers. both the enantiomers of alpha-amino-epsilon-caprolactam show an overshoot of the optical rotation during the enzymatic racemization in deuterium oxide (but not in water). this phenomenon may be attributable to ... | 1986 | 3955003 |
| activation of the alternative pathway of complement by an antitumor (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140, and its lower molecular weight and carboxymethylated derivatives. | an antitumor (1----3)-beta-d-glucan with a number-average degree of polymerization (dp) of 540 from alcaligenes faecalis var. myxogenes ifo 13140, and its lower molecular weight derivatives were found to activate the alternative pathway of complement (apc), as judged by hemolytic and immunoelectrophoretic analyses. of the native and derivative (1----3)-beta-d-glucans measured, the smallest one that showed apc-activating ability was that with a dp of about 20. the effect of carboxymethylation of ... | 1986 | 3957648 |
| comparison of cross-staining reactions by pseudomonas spp. and fluorescein-labeled polyclonal and monoclonal antibodies directed against legionella pneumophila. | commercially prepared polyclonal antisera to legionella pneumophila are known to cross-react with organisms of the genus pseudomonas. to determine whether a commercially available monoclonal antibody reagent specific for l. pneumophila would also cross-react with pseudomonads, a two-laboratory study was undertaken to test both monoclonal and polyclonal reagents against 33 isolates of pseudomonas spp., including 25 pseudomonas aeruginosa, 4 p. putida, 2 p. maltophilia, 1 p. fluorescens, and 1 p. ... | 1986 | 3958153 |
| investigation of cadmium resistance in an alcaligenes sp. | the mechanisms of metal resistance of a cadmium-resistant alcaligenes sp. were studied. growth in a defined medium was unaffected by cadmium at concentrations up to 0.1 mm, while at concentrations up to 2.5 mm, growth occurred after an extended lag phase. the increase in length of the lag phase was abolished by repeated subculturing at these higher concentrations. however, subculture in the absence of cadmium reversed the adaptation process. plasmid dna was not detected in adapted cells, suggest ... | 1986 | 3963810 |
| studies on microorganisms secured from different organs of slaughtered sheep with special reference to the microbial load in certain muscles. | a total of 312 samples collected from different organs and muscles of dead, disabled, diseased, dying, and normal carcasses of sheep were examined bacteriologically to determine the different species of microorganisms as to their public health significance. in addition, the aerobic plate count (apc) and most probable number (mpn) coliform/g were determined of musculus biceps brachii, musculus quadriceps femoris, and musculus longissimus dorsi. the mean bacterial load was higher in summer than in ... | 1986 | 3964203 |
| properties and electron transfer specificity of copper proteins from the denitrifier "achromobacter cycloclastes". | a blue copper protein (mr 12,000) was purified from cells of "achromobacter cycloclastes" grown as a denitrifier. when reduced, the blue copper protein transferred electrons to the copper protein nitrite reductase purified from the same cells, whereas a variety of cytochromes from denitrifiers failed to do so. inclusion of a protease inhibitor, phenylmethylsulfonyl fluoride, in the buffers employed during preparation yielded purified blue copper protein with 18 more amino acid residues and two t ... | 1986 | 3700338 |
| identification of oxidase-positive, glucose-negative, motile species of nonfermentative bacilli. | motile and glucose-negative nonfermentative bacilli are difficult to identify by commonly used procedures. we found eight features (reduction of nitrate and nitrite, acidification of fructose, and alkalinization of acetamide, arginine, histidine, saccharate, and urea) that identified 83% of 143 strains. these, along with additional biochemical tests, effected identification of 93% of the strains. hence, only a minority of these strains required examination by microscopy (for type of flagellation ... | 1986 | 3711277 |
| infections with achromobacter xylosoxidans. | 1986 | 3715491 | |
| antitumor polysaccharide-induced tumor-regressing factor in the serum of tumor-bearing mice: purification and characterization. | marked tumor-regressing activity was induced in the serum of s180 tumor-bearing mice by injection of an antitumor polysaccharide, cm-tak [carboxymethylated beta(1-3)glucan]. maximal activity was induced 7-14 days after the tumor transplantation and 10-12 h after cm-tak treatment. a quantitative assay for the activity was established on the basis of the initial decrease in the number of the tumor cells within 24 h. the factor with tumor-regressing activity was purified 10,000-fold by the series o ... | 1986 | 3723139 |
| [aniline oxidation by microorganisms in chemostat cultivation]. | under the conditions of chemostat cultivation, a mixed culture of microorganisms oxidized aniline at a gradually increasing concentration (up to 2.5 g/litre) as a sole source of carbon and nitrogen. the specific rate of aniline oxidation was as high as 160 mg per 1 g of dry biomass when aniline concentration in the growth medium was 2.5 g/litre. as aniline concentration in the growth medium was gradually raised, selection of the microorganisms took place and the number of the strains decreased f ... | 1986 | 3724566 |
| the efficacy of a temperature-sensitive mutant vaccine against northwest arkansas isolates of alcaligenes faecalis. | the efficacy of a commercially produced temperature-sensitive mutant alcaligenes faecalis vaccine was evaluated in turkeys contact-challenged with one of three strains of a. faecalis. in the vaccinated control group, the vaccine strain of a. faecalis colonized the nasal turbinates but not the trachea, caused no clinical signs of turkey coryza, and induced humoral antibodies. in the vaccinated challenged groups, the vaccine reduced both the severity of lesions and the number of birds exhibiting l ... | 1986 | 3729855 |
| tracheal mucus transport rate in normal turkeys and in turkeys infected with bordetella avium (alcaligenes faecalis). | using the radiopharmaceutical 99mtechnetium-sulfur colloid, the tracheal mucus transport rate (tmtr) was measured in healthy unanesthetized turkeys and in turkeys infected with bordetella avium. the tmtr of uninfected turkeys was 35.6 +/- 14.4 cm/min. the tmtr of b. avium-infected turkeys was normal on days 0 through 14 postexposure (pe), despite heavy bacterial colonization of the tracheal epithelium. on day 21 pe, the tmtr of b. avium-infected turkeys was significantly depressed (p less than o ... | 1986 | 3729858 |
| contribution to the taxonomy of the turkey coryza agent: cellular fatty acid analysis of the bacterium. | gas-liquid chromatography was used to analyze bacterial cellular fatty acids to elucidate the relatedness of the turkey coryza (tc) bacterium to alcaligenes spp., bordetella spp., and other gram-negative bacteria. the results indicated that the tc bacterium is not closely related to alcaligenes faecalis or any of the reference strains of alcaligenes and bordetella studied. most urease-positive bacterial isolates obtained from the upper respiratory tract of turkeys were identified as bordetella b ... | 1986 | 3729861 |
| transient kinetics of reduction of blue copper proteins by free flavin and flavodoxin semiquinones. | rate constants have been determined for the electron-transfer reactions between reduced free flavins and flavodoxin semiquinone and several blue copper proteins. correlations between these values and redox potentials demonstrate that spinach plastocyanin, pseudomonas aeruginosa azurin, alcaligenes sp. azurin, and alcaligenes sp. nitrite reductase have the same intrinsic reactivities toward free flavins, whereas stellacyanin is more reactive (3.3 times) and laccase considerably less reactive (app ... | 1986 | 3730365 |
| quantitative and qualitative studies of gut flora in striped bass from estuarine and coastal marine environments. | examination of the intestinal contents of 130 striped bass (morone saxatilis) collected from the hudson river and long island sound during may to october 1981 showed that opportunistic fish pathogens--especially aeromonas hydrophila--predominated in samples from both locations. other isolates from both groups of striped bass included vibrio, pseudomonads, flavo-bacteria, alcaligenes, and enterics. small numbers of micrococcus, bacillus, corynebacterium, and acinetobacter were also isolated. tota ... | 1986 | 3735580 |
| an evaluation of two methods of substrate alkalinization for the identification of bordetella avium and other similar organisms. | the effect of using two different techniques for the detection of substrate alkalinization by bordetella avium and reference strains of alcaligenes faecalis and b. bronchiseptica was evaluated. the techniques used were those described by otto and pickett and hinz et al. each test was in tubes sealed by either screw caps or cotton wool plugs. the alkalinization patterns obtained depended upon both the technique and the type of seal used. | 1986 | 3739213 |
| survey of disc diffusion antimicrobial sensitivity testing in avian bacteriology laboratories and the evaluation of a standardised method. | a survey was conducted in which 15 laboratories involved in avian bacteriology tested the antimicrobial sensitivity of cultures of staphylococcus aureus, s. epidermidis, escherichia coli, alcaligenes faecalis and salmonella typhimurium, using the disc diffusion test as routinely practised in each laboratory. up to 28 different antimicrobials or antimicrobial combinations were used. the most commonly tested agents were ampicillin, benzylpenicillin, chloramphenicol, erythromycin, furazolidone, neo ... | 1986 | 3741274 |
| purification and characterization of creatine amidinohydrolase of alcaligenes origin. | 1986 | 3742654 | |
| pathway of oxidation of pyruvic oxime by a heterotrophic nitrifier of the genus alcaligenes: evidence against nitroethane as an intermediate. | the role of nitroethane as an intermediate in the oxidation of pyruvic oxime to nitrate by an alcaligenes sp. was examined. unlike pyruvic oxime, which serves as a sole source of c and n for the bacterium, nitroethane was incapable of supporting the growth of the microbe. nitroethane was metabolized and diauxic growth did occur, however, if the nitroethane medium was amended with yeast extract. alcaligenes sp. resting cells and cell-free extracts were prepared from nitroethane-yeast extract grow ... | 1986 | 3767373 |
| peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans: case report and review of the literature. | we report the third human case of peritonitis caused by alcaligenes denitrificans subsp. xylosoxydans and review the english literature regarding community-acquired and nonsocomial infection and colonization that results from this bacterium. the biochemical and genetic characteristics supporting the pathogenic potential of a. denitrificans subsp. xylosoxydans are reviewed, and the antimicrobial susceptibility profile of the organism is summarized. | 1986 | 3771775 |
| microbial degradation of 1,3-dichlorobenzene. | a gram-negative, peritrichously flagellated rod, tentatively identified as an alcaligenes sp., was isolated from a mixture of soil and water samples by using 1,3-dichlorobenzene as the sole carbon and energy source. during growth on 1,3-dichlorobenzene, almost stoichiometric amounts of chloride were released. simultaneous adaptation studies, as well as enzyme studies, indicated that 1,3-dichlorobenzene was metabolized via 3,5-dichloro-cis-1,2-dihydroxycyclohexa-3,5-diene to 3,5-dichlorocatechol. ... | 1986 | 3777923 |
| isolation and characterization of aerobic heterotrophic bacteria from natural spring waters in the lanjaron area (spain). | aerobic, heterotrophic bacteria were isolated from nine natural mineral water springs in the lanjaron area of spain over the period july 1980 to may 1981. the mineral waters contained few bacteria (mean counts 26-5275 cfu per 100 ml) and the bacterial flora of all nine springs was very similar. most of the isolates were gram-negative rods (90%), and among these pseudomonas spp. and members of the flavobacterium-cytophaga-flexibacter group were numerically dominant. aeromonas-vibrio and enterobac ... | 1986 | 3781942 |
| preliminary characterization of crystals of nitrite reductase isolated from alcaligenes faecalis strain s-6. | crystals of the green, copper-containing enzyme nitrite reductase from alcaligenes faecalis s-6 have been obtained. they are monoclinic, space group p2(1) with a = 78.0 a, b = 92.9 a, c = 78.5 a and beta = 111.8 degrees. the crystals diffract to better than 2 a and are suitable for high-resolution x-ray crystallography. | 1986 | 3783698 |
| [amino acid metabolism by thin layer chromatography for the differentiation of bordetella and alcaligenes species]. | the amino acid metabolism of bordetella and alcaligenes strains was investigated for differentiation purposes. all species investigated reacted differently with a number of amino acids. the genus bordetella could be differentiated from that of alcaligenes by its inability to metabolize l-lysine, l-arginine and d-histidine. the recently described species bordetella avium, as well as achromobacter xylosoxidans (yabuuchi), considered as alcaligenes denitrificans subsp. xylosoxidans, presented thems ... | 1986 | 3788350 |
| degradation of 1,4-dichlorobenzene by alcaligenes sp. strain a175. | an organism, identified as an alcaligenes sp., was isolated from an enrichment culture in which 1,4-dichlorobenzene served as the sole carbon and energy source. during growth with 1,4-dichlorobenzene in pure culture, stoichiometric amounts of chloride were released. growth experiments and oxygen uptake rates with other chlorinated aromatic compounds revealed a high degree of specificity of the initial dioxygenase. cis-1,2-dihydroxycyclohexa-3,5-diene oxidoreductase and 1,2-pyrocatechase, but not ... | 1986 | 3789724 |
| oxygenase properties of the (4-hydroxybenzoyl)methanol-cleavage enzyme from an alcaligenes sp. | studies using h2(18)o and 18o2 demonstrated that, in the cleavage of (4-hydroxybenzoyl)methanol to 4-hydroxybenzoic and formic acids by an enzyme from an alicaligenes sp., oxygen is incorporated into both products from dioxygen and not from water. the enzyme is, therefore, an oxygenase. | 1986 | 3814084 |
| infections due to achromobacter xylosoxidans. case report and review of the literature. | achromobacter xylosoxidans is an uncommon nosocomial pathogen known to cause many serious infections. a 69-year-old woman with diabetes mellitus and chronic renal failure was admitted with pulmonary edema. the patient developed fever and pulmonary infiltrate with bilateral pleural effusions while she was on a respirator in the intensive care unit. culture of sputum, pleural fluid and blood grew a. xylosoxidans. bilateral chest tubes were inserted and the patient was treated for one month with pi ... | 1986 | 3818105 |
| the amino acid sequence of the blue copper protein of alcaligenes faecalis. | the complete amino acid sequence of a blue copper protein from alcaligenes faecalis s-6 has been determined. this protein is clearly homologous to pseudoazurins in achromobacter cycloclastes and pseudomonas am1, more distantly related to plant plastocyanins, and markedly different from the azurin of pseudomonas aeruginosa. yet all of these proteins bind copper, and analogous ligands appear to be involved. | 1986 | 3512305 |
| ribulose-bisphosphate carboxylase manipulation in the hydrogen bacterium alcaligenes eutrophus. | 1986 | 3514308 | |
| critical evaluation of the automicrobic system gram-negative identification card for identification of glucose-nonfermenting gram-negative rods. | during a 6-month study we critically evaluated the accuracy of the automicrobic system gram-negative identification card (vitek systems, inc., hazelwood, mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. of 356 isolates included in the automicrobic system profile, a total of 307 (86.2%) were correctly identified, 36 (10.1%) were not identified, and 13 (3.7%) were misidentified. fifty-eight of 63 (92% ... | 1986 | 3517050 |
| conjugal transfer of hydrogen-oxidizing ability of alcaligenes hydrogenophilus to pseudomonas oxalaticus. | conjugal transfer of hydrogen-oxidizing ability (hox) of the hydrogen bacterium alcaligenes hydrogenophilus was examined. intraspecific cross of plasmid phg21-a that encodes hydrogenases that mediate hydrogen oxidation was most frequent at 25 c; the optimal temperature for growth was 30 c. the plasmid could be transferred from a. hydrogenophilus to pseudomonas oxalaticus ox1 and ox4, and the resulting strains gained the capacity for autotrophic growth with h2 and co2. plasmid phg21-a was maintai ... | 1986 | 3521600 |
| isolation of hydrogen-oxidation gene from alcaligenes hydrogenophilus and its expression in pseudomonas oxalaticus. | a gene bank of a megaplasmid encoding the hydrogen-oxidizing enzyme system (hox) in alcaligenes hydrogenophilus was constructed using a broad host range cosmid vector pvk102, and established in escherichia coli. hybrid cosmids containing hox genes were identified by transferring the bank into pseudomonas oxalaticus ox1 and screening colonies for the ability of h2-dependent autotrophic growth. about 800 colonies were formed under autotrophic conditions. one of the hox+ transconjugants was isolate ... | 1986 | 3521601 |
| [subordination of the taxa of gram-negative bacteria determined by numerical analysis methods]. | various numerical methods were used to estimate the coordination of taxa of gram-negative aerobic and facultative anaerobic organoheterotrophic and chemolithotrophic bacteria. stable phena were found to be formed by cultures belonging to the families rhizobiaceae, halobacteriaceae, enterobacteriaceae, nitrobacteriaceae (except the genus nitrobacter), and methylomonadaceae (except the genus methylococcus). the unstable position was found in the genera thermus, zoogloea, xanthomonas, sulfolobus, m ... | 1986 | 3523170 |
| molecular cloning of structural and regulatory hydrogenase (hox) genes of alcaligenes eutrophus h16. | a gene bank of the 450-kilobase (kb) megaplasmid phg1 from the hydrogen-oxidizing bacterium alcaligenes eutrophus h16 was constructed in the broad-host-range mobilizable vector psup202 and maintained in escherichia coli. hox dna was identified by screening the e. coli gene bank for restoration of hydrogenase activity in a. eutrophus hox mutants. hybrid plasmids that contained an 11.6-kb ecori fragment restored soluble nad-dependent hydrogenase activity when transferred by conjugation into one cl ... | 1986 | 3536856 |
| comparison of rapid nft system and conventional methods for identification of nonsaccharolytic gram-negative bacteria. | this study examined the rapid nft system (analytab products, plainview, n.y.) to determine its ability to accurately identify 229 clinical isolates of mostly nonsaccharolytic gram-negative rods. identifications were classified by the following scheme: correct (corresponding to excellent, very good, good, or acceptable identification as listed in the code book); low discrimination (correct identification among a range of listed possibilities, with additional tests necessary for accurate identific ... | 1986 | 3536999 |
| enzymatic semisynthesis of [leub30] insulin. | experimental conditions for the preparation of [leub30] insulin by coupling of des-alab30 insulin with leu-obu(t) were determined using achromobacter protease i and trypsin as catalysts. successful coupling required a large excess of the amine component (0.8 m), a high concentration of organic cosolvent (35-50%) and neutral ph of the reaction mixture. the coupling yield of achromobacter protease i after 24 h at 37 degrees c was almost the same or a little higher than that at 25 degrees c. with t ... | 1986 | 3539841 |
| expression of the escherichia coli pfka gene in alcaligenes eutrophus and in other gram-negative bacteria. | the escherichia coli pfka gene has been cloned in the non-self-transmissible vector pvk101 from hybrid plasmids obtained from the clarke and carbon clone bank, resulting in the plasmids pas300 and pas100; the latter plasmid also encoded the e. coli tpi gene. these plasmids were transferred by conjugation to mutants of alcaligenes eutrophus which are unable to grow on fructose and gluconate due to lack of 2-keto-3-deoxy-6-phosphogluconate aldolase activity. these transconjugants recovered the abi ... | 1986 | 2937774 |
| in-vitro activity of newer quinolones against aerobic bacteria. | nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ... | 1986 | 2940214 |
| plasmid dna of virulent alcaligenes faecalis. | alcaligenes faecalis strains originating from chickens and from epizootics of coryza in turkeys were screened for antibiotic susceptibility and for the presence of plasmid dna. seven of 35 strains contained plasmid dna ranging in size from 10.5 to approximately 32 megadaltons. all of the strains isolated from turkeys were virulent in turkey poults, but only the plasmid-containing strains were resistant to sulfonamides and streptomycin. four of the plasmid-containing strains were also resistant t ... | 1986 | 3008603 |
| activation and active sites of nickel-containing hydrogenases. | hydrogenases that contain nickel and iron-sulphur clusters also have a regulatory mechanism, by which exposure to oxidants such as oxygen prevents their reaction with hydrogen. treatment with reducing agents then causes reactivation. in some hydrogenases from desulfovibrio species, there is evidence that there are at least two different deactivated states, which differ in their rates of reductive reactivation. the membrane-bound hydrogenase of d. desulfuricans, norway strain, the periplasmic hyd ... | 1986 | 3015251 |
| purification and some properties of cytochrome c' from a strain of achromobacter xylosoxidans. | cytochrome c' was crystallized from achromobacter xylosoxidans gifu 543. the cytochrome was a basic protein and its molecular weight was 28,000. the pyridine ferrohemochrome showed absorption peaks at 415, 521, and 551 nm. the absorption spectra of the oxidized and reduced forms at neutral ph were almost the same as those of other cytochromes c' reported already. the reduced cytochrome c' reacted with co and no, and the no complex showed a characteristic absorption spectrum. the midpoint redox p ... | 1986 | 3017925 |
| [peptide inhibitors of a bacterial collagenase]. | the properties of the cleavage site of collagen by collagenases remain discussed. the authors report their studies on the active site of the collagenase of a bacteria, achromobacter, based on two types of methods. the first type uses synthetic substrates, the second one enzymatic inhibitors. the results of both methods are reviewed. the inhibitors seem more sensitive than substrates to study the relation between structure and activity of the enzyme. | 1986 | 3019185 |