Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| prevalence of sos-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons. | abstract: | 2011 | 21529368 |
| chey3 of borrelia burgdorferi is the key response regulator essential for chemotaxis and forms a long-lived phosphorylated intermediate. | spirochetes have a unique cell structure: these bacteria have internal periplasmic flagella subterminally attached at each cell end. how spirochetes coordinate the rotation of the periplasmic flagella for chemotaxis is poorly understood. in other bacteria, modulating flagellar rotation is essential for chemotaxis, and phosphorylation-dephosphorylation of the response regulator chey plays a key role in regulating this rotary motion. the genome of the lyme disease spirochete borrelia burgdorferi c ... | 2011 | 21531807 |
| dendritic cells, antibodies reactive with oxldl, and inflammation. | periodontitis appears to promote chronic inflammatory diseases, including atherosclerosis, but relevant mechanisms need clarification. oral bacteria induce antibodies that bind not only bacteria, but also oxldl. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans induce remarkable igg responses that are dominated by igg2, and igg2 is ifn-γ-dependent and is promoted by dendritic cells (dcs). ldl-reactive antibodies induced by p. gingivalis and a. actinomycetemcomitans include anti- ... | 2011 | 21531918 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| the influence of micronutrients on oral and general health. | the aim of the present clinical pilot study was to examine the influence of a combination of micronutrients on individuals with high stress experience. | 2011 | 22027646 |
| periodontal disease and tooth loss as risks for cancer: a systematic review of the literature. | periodontal disease is a chronic destructive disease which occurs in adults, young people, and children. periodontal disease and periodontal pathogens have been associated with several systemic diseases and more recently, several studies have suggested the relationship between periodontal disease and cancer. studies with adjustment for the effect of smoking exposure, have found significant positive associations with different cancer sites. this review has outlined recent epidemiologic researches ... | 2011 | 26322197 |
| bacterial interactions in dental biofilm. | biofilms are masses of microorganisms that bind to and multiply on a solid surface, typically with a fluid bathing the microbes. the microorganisms that are not attached but are free floating in an aqueous environment are termed planktonic cells. traditionally, microbiology research has addressed results from planktonic bacterial cells. however, many recent studies have indicated that biofilms are the preferred form of growth of most microbes and particularly those of a pathogenic nature. biofil ... | 2011 | 21778817 |
| papillon-lefévre syndrome. | the goal of periodontal therapy has always been regeneration of the lost tissues. however, conventional periodontal therapy has not always been successful in achieving regeneration, especially when it is part of a syndrome. this case report involves a 13-year old male patient with the chief complaint of mobile teeth for over 3 months. his dental history revealed early loss of primary dentition, around 3-4 years of age and that he noticed mobility of permanent incisors and molars at 9-10 years. k ... | 2011 | 22144846 |
| measurement of peroxiredoxin activity. | peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. protocols are provided to measure prx activity with peroxide by (1) a coupled reaction with nadph, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the fox assay. | 2011 | 21818753 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| ros-mediated signalling in bacteria: zinc-containing cys-x-x-cys redox centres and iron-based oxidative stress. | bacteria are permanently in contact with reactive oxygen species (ros), both over the course of their life cycle as well that present in their environment. these species cause damage to proteins, lipids, and nucleotides, negatively impacting the organism. to detect these ros molecules and to stimulate the expression of proteins involved in antioxidative stress response, bacteria use a number of different protein-based regulatory and sensory systems. ros-based stress detection mechanisms induce p ... | 2011 | 21977318 |
| inflammasomes in cardiovascular diseases. | nod-like receptors (nlrs) constitute a recently identified family of macromolecules that participate in regulation of innate immune responses. to date, 23 members of the nlr family are identified in humans. diverse nlrs are stimulated by a broad range of pathogen- or danger-associated molecular patterns, and collectively function as intracellular pattern recognition receptors (prrs). the most studied inflammasomes are nlrp1 and nlrp3 that process inactive pro-caspase-1 to its active form, allowi ... | 2011 | 22254202 |
| benzyl isothiocyanate, a major component from the roots of salvadora persica is highly active against gram-negative bacteria. | plants produce a number of antimicrobial substances and the roots of the shrub salvadora persica have been demonstrated to possess antimicrobial activity. sticks from the roots of s. persica, miswak sticks, have been used for centuries as a traditional method of cleaning teeth. diverging reports on the chemical nature and antimicrobial repertoire of the chewing sticks from s. persica led us to explore its antibacterial properties against a panel of pathogenic or commensal bacteria and to identif ... | 2011 | 21829688 |
| phenytoin-induced gingival overgrowth: a review of the molecular, immune, and inflammatory features. | gingival overgrowth (go) is a side effect associated with some distinct classes of drugs, such as anticonvulsants, immunosuppressant, and calcium channel blockers. go is characterized by the accumulation of extracellular matrix in gingival connective tissues, particularly collagenous components, with varying degrees of inflammation. one of the main drugs associated with go is the antiepileptic phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. nevertheless, th ... | 2011 | 21991476 |
| evaluation of two mouth rinses in reduction of oral malodor using a spectrophotometric technique. | various mouth rinses have been used in the treatment of halitosis, but most of the evidence for the efficacy of mouth rinses is anecdotal. in the present study, 0.2% chlorhexidine rinse and an essential oil mouth rinse are compared for their efficacy in reducing the breath mercaptan levels. | 2011 | 22028512 |
| probiotics in periodontal health and disease. | macfarlane burnett stated in 1962 that "by the late twentieth century, we can anticipate the virtual elimination of infectious diseases as a significant factor in social life". probiotics have become of interest to researchers in recent times. time has come to shift the paradigm of treatment from specific bacteria elimination to altering bacterial ecology by probiotics. the development of resistance to a range of antibiotics by some important pathogens has raised the possibility of a return to t ... | 2011 | 21772717 |
| saliva as a diagnostic fluid. | salivary diagnostics is a dynamic and emerging field utilizing nanotechnology and molecular diagnostics to aid in the diagnosis of oral and systemic diseases. in this article the author critically reviews the latest advances using oral biomarkers for disease detection. the use of oral fluids is broadening perspectives in clinical diagnosis, disease monitoring, and decision making for patient care. important elements determining the future possibilities and challenges in this field are also discu ... | 2011 | 21094724 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| relationships among interleukin-6, tumor necrosis factor-α, adipokines, vitamin d, and chronic periodontitis. | the aim of this study is to explore relationships among serum adipokines, vitamin d, and clinical and microbial parameters of chronic periodontitis before and after treatment. | 2011 | 22181684 |
| bacterial biogeography of the human digestive tract. | we present bacterial biogeography as sampled from the human gastrointestinal tract of four healthy subjects. this study generated >32 million paired-end sequences of bacterial 16s rrna genes (v3 region) representing >95,000 unique operational taxonomic units (otus; 97% similarity clusters), with >99% good's coverage for all samples. the highest otu richness and phylogenetic diversity was found in the mouth samples. the microbial communities of multiple biopsy sites within the colon were highly s ... | 2011 | 22355685 |
| translational and clinical applications of salivary diagnostics. | there have been significant advances in techniques for the detection of biomarker signals in the oral cavity (e.g., elisas for proteins, pcr for rna and dna) as well as the engineering and development of microfluidic approaches to make oral-based point-of-care (poc) methods for the diagnosis for both local and systemic conditions a reality. in this section, we focus on three such approaches, namely, periodontal disease management, early markers for systemic diseases, and salivary markers useful ... | 2011 | 21917748 |
| neutrophils in periodontal inflammation. | neutrophils (also called polymorphonuclear leukocytes) are the most abundant leukocytes whose primary purpose as anti-microbial professional phagocytes is to kill extracellular pathogens. neutrophils and macrophages are phagocytic cell types that along with other cells effectively link the innate and adaptive arms of the immune response, and help promote inflammatory resolution and tissue healing. found extensively within the gingival crevice and epithelium, neutrophils are considered the key pr ... | 2011 | 22142957 |
| genomes of the most dangerous epidemic bacteria have a virulence repertoire characterized by fewer genes but more toxin-antitoxin modules. | we conducted a comparative genomic study based on a neutral approach to identify genome specificities associated with the virulence capacity of pathogenic bacteria. we also determined whether virulence is dictated by rules, or if it is the result of individual evolutionary histories. we systematically compared the genomes of the 12 most dangerous pandemic bacteria for humans ("bad bugs") to their closest non-epidemic related species ("controls"). | 2011 | 21437250 |
| pathologic crossroads: cardio-vascular diseases, periodontal diseases and calcium antagonists. | during the last decennium a more focused attention has been directed to the presence of chronic inflammation in cardiovascular diseases (cvd), but mainly to the high impact that this one has in generating and fastening the atherosclerotic process. | 2011 | 21505569 |
| periodontal treatment effects on endothelial function and cardiovascular disease biomarkers in subjects with chronic periodontitis: protocol for a randomized clinical trial. | periodontal disease (pd) is an infectious clinical entity characterized by the destruction of supporting tissues of the teeth as the result of a chronic inflammatory response in a susceptible host. it has been proposed that pd as subclinical infection may contribute to the etiology and to the pathogenesis of several systemic diseases including atherosclerosis. a number of epidemiological studies link periodontal disease/edentulism as independent risk factor for acute myocardial infarction, perip ... | 2011 | 21324167 |
| microbial shift and periodontitis. | 2011 | 21134227 | |
| evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity. | gleaning information regarding the molecular physiology of macromolecular complexes requires knowledge of their component stoichiometries. in this work, a relatively new means of analyzing sedimentation velocity (sv) data from the analytical ultracentrifuge is examined in detail. the method depends on collecting concentration profile data simultaneously using multiple signals, like rayleigh interferometry and uv spectrophotometry. if the cosedimenting components of a complex are spectrally disti ... | 2011 | 21256217 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| defining pathogenic bacterial species in the genomic era. | actual definitions of bacterial species are limited due to the current criteria of definition and the use of restrictive genetic tools. the 16s ribosomal rna sequence, for example, has been widely used as a marker for phylogenetic analyses; however, its use often leads to misleading species definitions. according to the first genetic studies, removing a certain number of genes from pathogenic bacteria removes their capacity to infect hosts. however, more recent studies have demonstrated that the ... | 2011 | 21687765 |
| changes in the subgingival biofilm composition after coronally positioned flap. | this study evaluated the effects of coronally positioned flap (cpf) on the subgingival biofilm composition. | 2011 | 21437473 |
| biofilm: a dental microbial infection. | recent advances in research technology have allowed researchers to study bacteria in their natural environment. dental biofilm forms via an ordered sequence of events, resulting in structured and functionally organized species rich microbial community and modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. sites for biofilm formation include all kinds of surfaces: natural materials above and below ... | 2011 | 22470238 |
| pulpal perio relations: interdisciplinary diagnostic approach - i. | lesions of pulpal and periodontal origin may perpetuate from either the infections of dental pulp or periodontium or alveolar bone. this review focuses on interdisciplinary diagnostic approach towards lesions of periodontal or endodontic origin. | 2011 | 21772729 |
| gingipains from porphyromonas gingivalis - complex domain structures confer diverse functions. | gingipains, a group of arginine or lysine specific cysteine proteinases (also known as rgpa, rgpb and kgp), have been recognized as major virulence factors in porphyromonas gingivalis. this bacterium is one of a handful of pathogens that cause chronic periodontitis. gingipains are involved in adherence to and colonization of epithelial cells, haemagglutination and haemolysis of erythrocytes, disruption and manipulation of the inflammatory response, and the degradation of host proteins and tissue ... | 2011 | 24466435 |
| critical analysis of biomarkers in the current periodontal practice. | periodontal disease is a chronic microbial infection that triggers inflammation-mediated loss of the periodontal ligament and alveolar bone that supports the teeth. because of the increasing prevalence and associated comorbidities, there is a need for the development of new diagnostic tests that can detect the presence of active disease, predict future disease progression, and evaluate the response to periodontal therapy, thereby improving the clinical management of periodontal patients. the dia ... | 2011 | 21976831 |
| ovine pedomics: the first study of the ovine foot 16s rrna-based microbiome. | we report the first study of the bacterial microbiome of ovine interdigital skin based on 16s rrna by pyrosequencing and conventional cloning with sanger-sequencing. three flocks were selected, one a flock with no signs of footrot or interdigital dermatitis, a second flock with interdigital dermatitis alone and a third flock with both interdigital dermatitis and footrot. the sheep were classified as having either healthy interdigital skin (h) and interdigital dermatitis (id) or virulent footrot ... | 2011 | 21430786 |
| clustering of subgingival microbial species in adolescents with periodontitis. | it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ... | 2011 | 21410554 |
| association of red complex, a. actinomycetemcomitans and non-oral bacteria with periodontal diseases. | pathogens related to systemic infections have been detected in the periodontal microbiota. the relationship amongst these pathogens, periodontal bacteria and periodontal clinical status is poorly understood. this study evaluated the association amongst red complex, a. actinomycetemcomitans (a.a) and non-oral pathogenic bacteria in subjects with good periodontal health (ph), gingivitis (g), chronic (cp) and aggressive (ap) periodontitis. | 2011 | 21397893 |
| short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms. | this study assessed the antibacterial activity of short-, medium-, and long-chain fatty acids against various oral microorganisms. | 2011 | 21333271 |
| effect of teenage smoking on the prevalence of periodontal bacteria. | the aim of our study was to investigate how teenage smoking affects the prevalence of periodontal bacteria and periodontal health with the hypothesis that smoking increases the prevalence of the bacteria. oral health of 264 adolescents (15- to 16-year-olds) was clinically examined, and their smoking history was recorded. the participants also filled in a structured questionnaire recording their general health and health habits. pooled subgingival plaque samples were taken for polymerase chain re ... | 2011 | 21340603 |
| rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms. | approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ... | 2011 | 21375703 |
| mycoplasma mycoides, from "mycoides small colony" to "capri". a microevolutionary perspective. | the mycoplasma mycoides cluster consists of five species or subspecies that are ruminant pathogens. one subspecies, mycoplasma mycoides subspecies mycoides small colony (mmmsc), is the causative agent of contagious bovine pleuropneumonia. its very close relative, mycoplasma mycoides subsp. capri (mmc), is a more ubiquitous pathogen in small ruminants causing mastitis, arthritis, keratitis, pneumonia and septicaemia and is also found as saprophyte in the ear canal. to understand the genetics unde ... | 2011 | 21324191 |
| identification of periodontal pathogens and severity of periodontitis in patients with and without chronic kidney disease. | in this study of patients with chronic periodontitis (cp), the severity of the disease and the main periodontal pathogens identified in patients with chronic kidney disease (ckd) were compared with those detected in individuals without systemic disease. | 2011 | 21211789 |
| comparison of gingival crevicular fluid sampling methods in patients with severe chronic periodontitis. | the analysis of samplings from periodontal pockets is important in the diagnosis and therapy of periodontitis. in this study, three different sampling techniques were compared to determine whether one method yielded samples suitable for the reproducible and simultaneous determination of bacterial load, cytokines, neutrophil elastase, and arginine-specific gingipains (rgps). rgps are an important virulence factor of porphyromonas gingivalis, the exact concentration of which in gingival crevicular ... | 2011 | 21235330 |
| effect of smoking on subgingival microflora of patients with periodontitis in japan. | smoking is a risk factor for periodontitis. to clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. the aim of this study was to gain an insight into the influence of smoking on the microflora of japanese patients with periodontitis. | 2011 | 21208407 |
| prognosis of periodontitis recurrence after intensive periodontal treatment using examination of serum igg antibody titer against periodontal bacteria. | chronic periodontitis is associated with systemic diseases such as atherosclerosis. in this study, we evaluated the efficacy of serum igg antibody titer to periodontal bacteria for prognosis of periodontitis recurrence during supportive periodontal therapy (spt) phase. the 139 patients during spt phase were selected and divided to two groups as follows: "stable" and "recurrence" group at spt phase for case-control study: "high igg titer" and "normal igg titer" group before transition to spt phas ... | 2011 | 21254239 |
| a selenium-dependent xanthine dehydrogenase triggers biofilm proliferation in enterococcus faecalis through oxidant production. | selenium has been shown to be present as a labile cofactor in a small class of molybdenum hydroxylase enzymes in several species of clostridia that specialize in the fermentation of purines and pyrimidines. this labile cofactor is poorly understood, yet recent bioinformatic studies have suggested that enterococcus faecalis could serve as a model system to better understand the way in which this enzyme cofactor is built and the role of these metalloenzymes in the physiology of the organism. an mr ... | 2011 | 21257770 |
| molecular survey of atheromatous plaques for the presence of dna from periodontal bacterial pathogens, archaea and fungi. | chronic infections, such as periodontitis, have been associated with the development and progression of atherosclerosis. the mechanisms through which this occurs have yet to be elucidated. this study was carried out to detect periodontopathic bacteria as well as archaea and fungi in atheromatous plaques and search for factors associated with their occurrence in atheromas. | 2011 | 21261623 |
| polymicrobial infection with periodontal pathogens specifically enhances microrna mir-146a in apoe-/- mice during experimental periodontal disease. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia are periodontal pathogens associated with the etiology of adult periodontitis as polymicrobial infections. recent studies demonstrated that oral infection with p. gingivalis induces both periodontal disease and atherosclerosis in hyperlipidemic and proatherogenic apoe(-/-) mice. in this study, we explored the expression of micrornas (mirnas) in maxillas (periodontium) and spleens isolated from apoe(-/-) mice infected with p. ... | 2011 | 21263019 |
| comparison of real-time polymerase chain reaction and dna-strip technology in microbiological evaluation of periodontitis treatment. | the impact of a semiquantitative commercially available test based on dna-strip technology (microident®, hain lifescience, nehren, germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time pcr. subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. after extracting dna, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsyth ... | 2011 | 21146709 |
| regulation of tlr2-mediated tolerance and cross-tolerance through irak4 modulation by mir-132 and mir-212. | innate immune response is the first defense against pathogens via recognition by various conserved pattern recognition receptors, such as tlrs, to initiate a rapid and strong cytokine alarm. tlr signaling-mediated cytokine production must be properly regulated to prevent pathological conditions deriving from overproduction of cytokines. in this study, the role of specific micrornas in tlr-signaling pathway was investigated to reveal the cross-interaction and -regulation in the myd88 pathway. in ... | 2012 | 23264652 |
| regulation of tlr2-mediated tolerance and cross-tolerance through irak4 modulation by mir-132 and mir-212. | innate immune response is the first defense against pathogens via recognition by various conserved pattern recognition receptors, such as tlrs, to initiate a rapid and strong cytokine alarm. tlr signaling-mediated cytokine production must be properly regulated to prevent pathological conditions deriving from overproduction of cytokines. in this study, the role of specific micrornas in tlr-signaling pathway was investigated to reveal the cross-interaction and -regulation in the myd88 pathway. in ... | 2012 | 23264652 |
| multiplicity of salmonella entry mechanisms, a new paradigm for salmonella pathogenesis. | the salmonella enterica species includes about 2600 diverse serotypes, most of which cause a wide range of food- and water-borne diseases ranging from self-limiting gastroenteritis to typhoid fever in both humans and animals. moreover, some serotypes are restricted to a few animal species, whereas other serotypes are able to infect plants as well as cold- and warm-blooded animals. an essential feature of the pathogenicity of salmonella is its capacity to cross a number of barriers requiring inva ... | 2012 | 23170225 |
| identification of a unique tlr2-interacting peptide motif in a microbial leucine-rich repeat protein. | pathogenesis of many bacterially-induced inflammatory diseases is driven by toll-like receptor (tlr) mediated immune responses following recognition of bacterial factors by different tlrs. periodontitis is a chronic inflammation of the tooth supporting apparatus often leading to tooth loss, and is caused by a gram-negative bacterial consortium that includes tannerella forsythia. this bacterium expresses a virulence factor, the bspa, which drives periodontal inflammation by activating tlr2. the n ... | 2012 | 22695115 |
| recent progress in synthetic biology for microbial production of c3-c10 alcohols. | the growing need to address current energy and environmental problems has sparked an interest in developing improved biological methods to produce liquid fuels from renewable sources. while microbial ethanol production is well established, higher-chain alcohols possess chemical properties that are more similar to gasoline. unfortunately, these alcohols (except 1-butanol) are not produced efficiently in natural microorganisms, and thus economical production in industrial volumes remains a challen ... | 2012 | 22701113 |
| occurrence of periodontal pathogens among patients with chronic periodontitis. | the aim of the present study was to evaluate the presence of the periodontal pathogens that form the red complex (tannerella forsythia, porphyromonas gingivalis and treponema denticola) and aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. the sample consisted of 29 patients with a clinical and radiographic diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology (3). samples for microbiological analysis were collected from th ... | 2012 | 24031906 |
| insights into the virulence of oral biofilms: discoveries from proteomics. | this review covers developments in the study of polymicrobial communities, biofilms and selected areas of host response relevant to dental plaque and related areas of oral biology. the emphasis is on recent studies in which proteomic methods, particularly those using mass spectrometry as a readout, have played a major role in the investigation. the last 5-10 years have seen a transition of such methods from the periphery of oral biology to the mainstream, as in other areas of biomedical science. ... | 2012 | 22809209 |
| co-localized or randomly distributed? pair cross correlation of in vivo grown subgingival biofilm bacteria quantified by digital image analysis. | the polymicrobial nature of periodontal diseases is reflected by the diversity of phylotypes detected in subgingival plaque and the finding that consortia of suspected pathogens rather than single species are associated with disease development. a number of these microorganisms have been demonstrated in vitro to interact and enhance biofilm integration, survival or even pathogenic features. to examine the in vivo relevance of these proposed interactions, we extended the spatial arrangement analy ... | 2012 | 22655057 |
| causes of adverse pregnancy outcomes and the role of maternal periodontal status - a review of the literature. | preterm (pt) and low birth weight (lbw) are considered to be the most relevant biological determinants of newborn infants survival, both in developed and in developing countries. numerous risk factors for pt and lbw have been defined in the literature. infections of the genitourinary tract infections along with various biological and genetic factors are considered to be the most common etiological factors for pt/lbw deliveries. however, evidence suggests that sub-clinical infection sites that ar ... | 2012 | 22615718 |
| relationship between oral anaerobic bacteria and otitis media with effusion. | in this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (ome), we aimed to investigate the presence and similarity of fusobacterium nucleatum and treponema denticola in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with ome. | 2012 | 22606045 |
| spontaneous periodontitis development in diabetic rats involves an unrestricted expression of inflammatory cytokines and tissue destructive factors in the absence of major changes in commensal oral microbiota. | diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia is a main feature. the objective was to evaluate the involvement of rage, inflammatory cytokines, and metalloproteinases in spontaneous periodontitis triggered by diabetes induction. immunohistochemical procedures for mmp-2, mmp-9, tnf-α, il-1β, il-6, rankl, and rage were performed in rats after 1, 3, 6, 9, and 12 months of diabetes induction. total dna was extracted from paraffin-embedded tissues and evaluated by re ... | 2012 | 22611374 |
| identification of an intracellular m17 family leucine aminopeptidase that is required for virulence in staphylococcus aureus. | staphylococcus aureus is a highly virulent bacterial pathogen capable of causing a variety of ailments throughout the human body. it is a major public health concern due to the continued emergence of highly pathogenic methicillin resistant strains (mrsa) both within hospitals and in the community. virulence in s. aureus is mediated by an array of secreted and cell wall associated virulence factors, including toxins, hemolysins and proteases. in this work we identify a leucine aminopeptidase (lap ... | 2012 | 22613209 |
| serum antibodies to periodontal pathogens are a risk factor for alzheimer's disease. | chronic inflammation in periodontal disease has been suggested as a potential risk factor in alzheimer's disease (ad). the purpose of this study was to examine serum antibody levels to bacteria of periodontal disease in participants who eventually converted to ad compared with the antibody levels in control subjects. | 2012 | 22546352 |
| prevalence of fima genotypes of porphyromonas gingivalis and other periodontal bacteria in a spanish population with chronic periodontitis. | the aim of this study was to determine the prevalence of the different fima genotypes of porphyromonas gingivalis in adult spanish patients with chronic periodontitis, patients with gingivitis and periodontally healthy subjects, and the relationship between these genotypes and other periodontopathogenic bacteria. | 2012 | 22549664 |
| tprc/d (tp0117/131), a trimeric, pore-forming rare outer membrane protein of treponema pallidum, has a bipartite domain structure. | identification of treponema pallidum rare outer membrane proteins (omps) has been a longstanding objective of syphilis researchers. we recently developed a consensus computational framework that employs a battery of cellular localization and topological prediction tools to generate ranked clusters of candidate rare omps (d. l. cox et al., infect. immun. 78:5178-5194, 2010). tp0117/tp0131 (tprc/d), a member of the t. pallidum repeat (tpr) family, was a highly ranked candidate. circular dichroism, ... | 2012 | 22389487 |
| oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. | oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (hsct). through hsct, the homeostasis in the oral cavity is disrupted. the contribution of the oral microflora to mucositis remains to be clarified. the aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in hsct recipients. | 2012 | 22531876 |
| regulation of mnth by a dual mn(ii)- and fe(ii)-dependent transcriptional repressor (dr2539) in deinococcus radiodurans. | the high intracellular mn/fe ratio observed within the bacteria deinococcus radiodurans may contribute to its remarkable resistance to environmental stresses. we isolated dr2539, a novel regulator of intracellular mn/fe homeostasis in d. radiodurans. electrophoretic gel mobility shift assays (emsas) revealed that dr2539 binds specifically to the promoter of the manganese acquisition transporter (mnth) gene, and that dr0865, the only fur homologue in d. radiodurans, cannot bind to the promoter of ... | 2012 | 22523570 |
| acquisition of complement inhibitor serine protease factor i and its cofactors c4b-binding protein and factor h by prevotella intermedia. | infection with the gram-negative pathogen prevotella intermedia gives rise to periodontitis and a growing number of studies implies an association of p. intermedia with rheumatoid arthritis. the serine protease factor i (fi) is the central inhibitor of complement degrading complement components c3b and c4b in the presence of cofactors such as c4b-binding protein (c4bp) and factor h (fh). yet, the significance of complement inhibitor acquisition in p. intermedia infection and fi binding by gram-n ... | 2012 | 22514678 |
| papillon-lefèvre syndrome: case report and review of the literature. | papillon-lefèvre syndrome is a very rare syndrome of autosomal recessive inheritance characterized by palmar-plantar hyperkeratosis and early onset of a severe destructive periodontitis leading to premature loss of both primary and permanent dentitions. various etiopathogenic factors are associated with the syndrome; but a recent report has suggested that the condition is linked to mutations of the cathepsin c gene. two cases of papillon-lefèvre syndrome in the same family, having all of the cha ... | 2012 | 23055596 |
| association between interleukin-1 gene polymorphism and severity of chronic periodontitis in a south indian population group. | periodontitis is a bacterial disease modified by multiple factors. interleukin-1 (il-1) is a key regulator of the host response and a major modulator of extracellular matrix catabolism and bone resorption. it has been reported that variations in il-1 gene are associated with increased susceptibility to periodontitis. the aims of the study were 1) to analyze the distribution of single nucleotide polymorphism of il-1 (il-1a-+4845 and il-1b-+3954) and 2) to correlate the association of the composit ... | 2012 | 23055581 |
| conservation and revised annotation of the treponema denticola prcb-prca-prtp locus encoding the dentilisin (ctlp) protease complex. | interstrain differences in antigenic surface proteins may reflect immunological pressure or differences in receptor specificity of the antigen. treponema denticola exhibits considerable interstrain variability in its major surface protein (msp), but no studies have addressed this issue in dentilisin (ctlp), a surface protease complex that has a significant role in t. denticola-host interactions in periodontal disease. furthermore, the genome annotation of the prcb-prca-prtp operon encoding denti ... | 2012 | 23253337 |
| conservation and revised annotation of the treponema denticola prcb-prca-prtp locus encoding the dentilisin (ctlp) protease complex. | interstrain differences in antigenic surface proteins may reflect immunological pressure or differences in receptor specificity of the antigen. treponema denticola exhibits considerable interstrain variability in its major surface protein (msp), but no studies have addressed this issue in dentilisin (ctlp), a surface protease complex that has a significant role in t. denticola-host interactions in periodontal disease. furthermore, the genome annotation of the prcb-prca-prtp operon encoding denti ... | 2012 | 23253337 |
| hcpr of porphyromonas gingivalis is required for growth under nitrosative stress and survival within host cells. | although the gram-negative, anaerobic periodontopathogen porphyromonas gingivalis must withstand nitrosative stress, which is particularly high in the oral cavity, the mechanisms allowing for protection against such stress are not known in this organism. in this study, microarray analysis of p. gingivalis transcriptional response to nitrite and nitric oxide showed drastic upregulation of the pg0893 gene coding for hybrid cluster protein (hcp), which is a putative hydroxylamine reductase. althoug ... | 2012 | 22778102 |
| global expression profile of biofilm resistance to antimicrobial compounds in the plant-pathogenic bacterium xylella fastidiosa reveals evidence of persister cells. | investigations of biofilm resistance response rarely focus on plant-pathogenic bacteria. since xylella fastidiosa is a multihost plant-pathogenic bacterium that forms biofilm in the xylem, the behavior of its biofilm in response to antimicrobial compounds needs to be better investigated. we analyzed here the transcriptional profile of x. fastidiosa subsp. pauca in response to inhibitory and subinhibitory concentrations of copper and tetracycline. copper-based products are routinely used to contr ... | 2012 | 22730126 |
| metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study. | this study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. dna was extracted from subgingival samples and subjected to pcr amplification of the c2-c4 regions of the 16s rdna using primers with bar codes to identify individual samples. the pcr products were pooled and sequenced for the v4 region of the 16s rdna using the 454 flx standard platform. the results were analyzed f ... | 2012 | 23341849 |
| structure of the essential diversity-generating retroelement protein bavd and its functionally important interaction with reverse transcriptase. | diversity-generating retroelements (dgrs) are the only known source of massive protein sequence variation in prokaryotes. these elements transfer coding information from a template region (tr) through an rna intermediate to a protein-encoding variable region. this retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical bpp-1 dgr, requires a reverse transcriptase (brt) and an accessory variability determinant (bavd) protein. to understand the role of bav ... | 2012 | 23273427 |
| treponema denticola interactions with host proteins. | oral treponema species, most notably t. denticola, are implicated in the destructive effects of human periodontal disease. progress in the molecular analysis of interactions between t. denticola and host proteins is reviewed here, with particular emphasis on the characterization of surface-expressed and secreted proteins of t. denticola involved in interactions with host cells, extracellular matrix components, and components of the innate immune system. | 2012 | 22368767 |
| involvement of a periodontal pathogen, porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease. | non-alcoholic fatty liver disease (nafld) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. however, other risk factors for the development of nafld are unclear. with the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between nafld and infection with porphyro ... | 2012 | 22340817 |
| microbiologic profile of endodontic infections from hiv- and hiv+ patients using multiple-displacement amplification and checkerboard dna-dna hybridization. | to compare the microbiota of endodontic infections in necrotic pulp from hiv-negative and hiv-positive subjects. | 2012 | 22335194 |
| beta-hexosaminidase activity of the oral pathogen tannerella forsythia influences biofilm formation on glycoprotein substrates. | tannerella forsythia is an important pathogen in periodontal disease. previously, we showed that its sialidase activity is key to utilization of sialic acid from a range of human glycoproteins for biofilm growth and initial adhesion. removal of terminal sialic acid residues often exposes β-linked glucosamine or galactosamine, which may also be important adhesive molecules. in turn, these residues are often removed by a group of enzymes known as β-hexosaminidases. we show here that t. forsythia h ... | 2012 | 22276920 |
| rheumatoid arthritis and periodontitis - inflammatory and infectious connections. review of the literature. | an association between oral disease/periodontitis and rheumatoid arthritis (ra) has been considered since the early 1820s. the early treatment was tooth eradication. epidemiological studies suggest that the prevalence of ra and periodontitis may be similar and about 5% of the population are aged 50 years or older. ra is considered as an autoimmune disease whereas periodontitis has an infectious etiology with a complex inflammatory response. both diseases are chronic and may present with bursts o ... | 2012 | 22347541 |
| analysis of the salivary microbiome using culture-independent techniques. | the salivary microbiota is a potential diagnostic indicator of several diseases. culture-independent techniques are required to study the salivary microbial community since many of its members have not been cultivated. | 2012 | 22300522 |
| disabled patients and oral health in rome, italy: long-term evaluation of educational initiatives. | this study is concerned with the educational intervention layout proposed as a possible answer for the disparities in healthcare services for disabled persons. | 2012 | 22545186 |
| disabled patients and oral health in rome, italy: long-term evaluation of educational initiatives. | this study is concerned with the educational intervention layout proposed as a possible answer for the disparities in healthcare services for disabled persons. | 2012 | 22545186 |
| a metalloproteinase karilysin present in the majority of tannerella forsythia isolates inhibits all pathways of the complement system. | tannerella forsythia is a poorly studied pathogen despite being one of the main causes of periodontitis, which is an inflammatory disease of the supporting structures of the teeth. we found that despite being recognized by all complement pathways, t. forsythia is resistant to killing by human complement, which is present at up to 70% of serum concentration in gingival crevicular fluid. incubation of human serum with karilysin, a metalloproteinase of t. forsythia, resulted in a decrease in bacter ... | 2012 | 22287711 |
| analysis of the cell surface layer ultrastructure of the oral pathogen tannerella forsythia. | the gram-negative oral pathogen tannerella forsythia is decorated with a 2d crystalline surface (s-) layer, with two different s-layer glycoprotein species being present. prompted by the predicted virulence potential of the s-layer, this study focused on the analysis of the arrangement of the individual s-layer glycoproteins by a combination of microscopic, genetic, and biochemical analyses. the two s-layer genes are transcribed into mrna and expressed into protein in equal amounts. the s-layer ... | 2012 | 22273979 |
| structure of the essential diversity-generating retroelement protein bavd and its functionally important interaction with reverse transcriptase. | diversity-generating retroelements (dgrs) are the only known source of massive protein sequence variation in prokaryotes. these elements transfer coding information from a template region (tr) through an rna intermediate to a protein-encoding variable region. this retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical bpp-1 dgr, requires a reverse transcriptase (brt) and an accessory variability determinant (bavd) protein. to understand the role of bav ... | 2012 | 23273427 |
| oral spirochetes implicated in dental diseases are widespread in normal human subjects and carry extremely diverse integron gene cassettes. | the nih human microbiome project (hmp) has produced several hundred metagenomic data sets, allowing studies of the many functional elements in human-associated microbial communities. here, we survey the distribution of oral spirochetes implicated in dental diseases in normal human individuals, using recombination sites associated with the chromosomal integron in treponema genomes, taking advantage of the multiple copies of the integron recombination sites (repeats) in the genomes, and using a ta ... | 2012 | 22635997 |
| microbial interactions in building of communities. | establishment of a community is considered to be essential for microbial growth and survival in the human oral cavity. biofilm communities have increased resilience to physical forces, antimicrobial agents and nutritional variations. specific cell-to-cell adherence processes, mediated by adhesin-receptor pairings on respective microbial surfaces, are able to direct community development. these interactions co-localize species in mutually beneficial relationships, such as streptococci, veillonell ... | 2012 | 23253299 |
| bacterial community shift in treated periodontitis patients revealed by ion torrent 16s rrna gene amplicon sequencing. | periodontitis, one of the most common diseases in the world, is caused by a mixture of pathogenic bacteria and inflammatory host responses and often treated by antimicrobials as an adjunct to scaling and root planing (srp). our study aims to elucidate explorative and descriptive temporal shifts in bacterial communities between patients treated by srp alone versus srp plus antibiotics. this is the first metagenomic study using an ion torrent personal genome machine (pgm). eight subgingival plaque ... | 2012 | 22870235 |
| microbial interactions in building of communities. | establishment of a community is considered to be essential for microbial growth and survival in the human oral cavity. biofilm communities have increased resilience to physical forces, antimicrobial agents and nutritional variations. specific cell-to-cell adherence processes, mediated by adhesin-receptor pairings on respective microbial surfaces, are able to direct community development. these interactions co-localize species in mutually beneficial relationships, such as streptococci, veillonell ... | 2012 | 23253299 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| teth137, a conserved factor of unknown function from thermoanaerobacter ethanolicus jw200, represses the transcription of the adhe gene in vitro. | teth137, a 13.7 kd protein of unknown function from thermoanaerobacter ethanolicus jw200, is encoded by 360 nucleotides and has been obtained by dna-coupled column previously. however, no function study of teth137 has been published. homologous modeling of teth137 shows the protein is comprised of a helix-turn-helix motif which is a typical dna-binding domain. therefore, it is speculated teth137 is a dna-binding protein and involved in transcription of the adhe gene (encodes alcohol dehydrogenas ... | 2012 | 24426101 |
| association of cd14-260 polymorphisms, red-complex periodontopathogens and gingival crevicular fluid cytokine levels with cyclosporine a-induced gingival overgrowth in renal transplant patients. | backgroud and objective: genetic factors may influence the colonization of pathogenic bacteria, therefore increasing the risk for the initiation and development of periodontal disease. the present study was carried out to investigate the association of cd14-260 polymorphisms, subgingival microbiota, and gingival crevicular fluid (gcf) cytokine levels with cyclosporine a (csa)-induced gingival overgrowth (go) in renal transplant patients. | 2012 | 22934794 |
| papillon-lefèvre syndrome: a series of six cases in the same family. | papillon-lefèvre syndrome (pls) is a rare, autosomal recessive heterogeneous disorder, which is characterized by palmoplantar hyperkeratosis, early loss of primary and permanent teeth, and associated calcification of the dura mater. herein we described six cases of pls in the same family. in this series, six cases (two females and four males) with the mean age of 15.6 ± 10.4 years were recruited. palmoplantar hyperkeratosis was detected in all of the cases, leading to a difficult and painful wal ... | 2012 | 23251811 |
| association of cd14-260 polymorphisms, red-complex periodontopathogens and gingival crevicular fluid cytokine levels with cyclosporine a-induced gingival overgrowth in renal transplant patients. | backgroud and objective: genetic factors may influence the colonization of pathogenic bacteria, therefore increasing the risk for the initiation and development of periodontal disease. the present study was carried out to investigate the association of cd14-260 polymorphisms, subgingival microbiota, and gingival crevicular fluid (gcf) cytokine levels with cyclosporine a (csa)-induced gingival overgrowth (go) in renal transplant patients. | 2012 | 22934794 |
| bacterial cell surface heterogeneity: a pathogen's disguise. | 2012 | 22952444 | |
| bugs that debugs: probiotics. | the oral cavity harbors a diverse array of bacterial species. there are more than 600 species that colonize in the oral cavity. these include a lot of organisms that are not commonly known to reside in the gastrointestinal (gi) tract and also are more familiar: lactobacillus acidophilus, lactobacillus casei, lactobacillus fermentum, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius. the balance of all these microorganisms can easily be disturbed and a prevalence of p ... | 2012 | 23066281 |
| staphylococcal superantigen-like protein 3 binds to the toll-like receptor 2 extracellular domain and inhibits cytokine production induced by staphylococcus aureus, cell wall component, or lipopeptides in murine macrophages. | staphylococcal superantigen-like proteins (ssls) are a family of exoproteins sharing structural similarity with superantigens, but no superantigenic activity. corresponding host target proteins or receptors against a portion of ssls in the family have been identified. in this study, we show that ssl3 specifically binds to toll-like receptor 2 (tlr2) and inhibits the stimulation of macrophages by tlr2 ligands. an approximately 100-kda protein was recovered by using recombinant his-tagged ssl3-con ... | 2012 | 22665377 |
| genome-wide association study of periodontal pathogen colonization. | pathological shifts of the human microbiome are characteristic of many diseases, including chronic periodontitis. to date, there is limited evidence on host genetic risk loci associated with periodontal pathogen colonization. we conducted a genome-wide association (gwa) study among 1,020 white participants of the atherosclerosis risk in communities study, whose periodontal diagnosis ranged from healthy to severe chronic periodontitis, and for whom "checkerboard" dna-dna hybridization quantificat ... | 2012 | 22699663 |
| treponema denticola superoxide reductase: in vivo role, in vitro reactivities, and a novel [fe(cys)(4)] site. | in vitro and in vivo results are presented demonstrating that superoxide reductase (sor) from the air-sensitive oral spirochete, treponema denticola (td), is a principal enzymatic scavenger of superoxide in this organism. this sor contains the characteristic non-heme [fe(his)(4)cys] active sites. no other metal-binding domain has been annotated for td sor. however, we found that td sor also accommodates a [fe(cys)(4)] site whose spectroscopic and redox properties resemble those in so-called 2fe- ... | 2012 | 22715932 |