Publications

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real-time investigation of antibiotics-induced oxidative stress and superoxide release in bacteria using an electrochemical biosensor.the involvement of oxidative stress in the mechanism of antibiotics-meditated cell death is unclear and subject to debate. the kinetic profile and a quantitative relationship between the release of reactive oxygen species (ros), bacteria and antibiotic type remain elusive. here we report direct measurements and analytical quantification of the release of superoxide radicals (o2(·-)), a major contributor to ros, in antibiotics-treated bacterial cultures using a cytochrome c electrochemical biosen ...201626655038
biofabrication of zinc oxide nanoparticles using fruit extract of rosa canina and their toxic potential against bacteria: a mechanistic approach.the use of plant extract in the biosynthesis of nanoparticles (nps) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. in this study, the biosynthesis of zinc oxide (zno) nps by both "conventional heating" (ch) and "microwave irradiation" (mi) methods has been reported. stable and spherical znonps were produced using zinc nitrate and flesh extract of rosa canina fruit (rosehip) which was used as a precurso ...201626652376
bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions.clove oil (co) anionic nanoemulsions were prepared with varying ratios of co to canola oil (ca), emulsified and stabilized with purity gum ultra (pgu), a newly developed succinylated waxy maize starch. interfacial tension measurements showed that co acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5:5% v/v co:ca) was reached. antimicrobial activity of the negatively charge ...201626616926
the effect of nacl reduction in the microbiological quality of cracked green table olives of the maçanilha algarvia cultivar.the present work aimed at studying the effect of the partial replacement of nacl with kcl and cacl2 of the fermenting brines on the microbiological quality of natural cracked green maçanilha algarvia table olives. olives were fermented in different salt combinations (brine 1-8% nacl, brine 2-4% nacl 4% kcl, brine 3-4% nacl 4% cacl2, brine 4-4% kcl 4% cacl2, and brine 5-2.7% nacl 2.7% kcl 2.7% cacl2) and the abundance of yeasts and enterobacteria was determined. at the end of fermentation, the ma ...201626613162
cationic nanostructures against foodborne pathogens. 201627881979
antibacterial potential of jatropha curcas synthesized silver nanoparticles against food borne pathogens.the aqueous leaf extract of jatropha curcas was used for the synthesis of silver nanoparticles (jc-agnps) which were further evaluated for its antibacterial potential against food borne pathogens. j. curcas leaf extract could synthesize stable silver nanoparticles (zeta potential: -23.4 mv) with absorption band at 430 nm. fourier transform infrared spectroscopy indicated various biological compounds responsible for capping and stabilizing jc-agnps in suspension, while the presence of silver was ...201627877160
microbial quality and suitability of roof-harvested rainwater in rural villages for crop irrigation and domestic use.the study aimed at assessing the microbiological quality and suitability of roof-harvested rainwater (rhrw) for crop irrigation and domestic use. in total, 80 rainwater tanks (246 samples) across three rural villages (ga-molepane, jericho and luthngele) were visited. culture-based techniques were used to isolate bacterial microbes and identities were confirmed using matrix-assisted laser desorption/ionization time of flight (maldi-tof-ms). uncultured fungal populations were also identified using ...201627959874
microfluidic air sampler for highly efficient bacterial aerosol collection and identification.the early warning capability of the presence of biological aerosol threats is an urgent demand in ensuing civilian and military safety. efficient and rapid air sample collection in relevant indoor or outdoor environment is a key step for subsequent analysis of airborne microorganisms. herein, we report a portable battery-powered sampler that is capable of highly efficient bioaerosol collection. the essential module of the sampler is a polydimethylsiloxane (pdms) microfluidic chip, which consiste ...201627934096
microbiological and chemico-physical shelf-life and panel test to evaluate acceptability of liver mortadella.this study aimed to evaluate the shelf life of sliced cooked liver mortadella packaged in map (70-85% n2, 15-30% co2) and stored in refrigeration (4°c) or slight thermal abuse (8°c) for up to 49 days (declared best before date 45 days). the proximate composition, aw nitrites and nacl content were determined at t0. weekly, samples were submitted to microbiological [total viable count (tvc), lactic acid bacteria (lab), enterobacteriaceae, escherichia coli, pseudomonas spp., coagulase positive stap ...201628058249
evaluation of molecular typing of foodborne pathogens in european reference laboratories from 2012 to 2013.in 2012, the european centre for disease prevention and control (ecdc) initiated external quality assessment (eqa) schemes for molecular typing including the national public health reference laboratories in europe. the overall aim for these eqa schemes was to enhance the european surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. the eqas were organised by statens serum institut (ssi) and included salmonella enterica subsp. enteric ...201628006653
evaluation of hygienic quality and labelling of fish distributed in public canteens of northeast italy.over the past few years, the demand for the introduction of fish products in public canteens (schools, hospitals and nursing-homes) has grown due to their good nutritional proprieties. the particular health conditions and sensitivity of some groups of consumers exposes them to greater risks of food poisoning. it is therefore important to monitor the raw materials that end up in mass catering implementing strategies of mass catering control, both with self-monitoring strategies and with regular c ...201627995098
in vitro evaluation of bacteriocin-like inhibitory substances produced by lactic acid bacteria isolated during traditional sicilian cheese making.bacteriocins are antimicrobial proteins produced by bacteria that inhibit the growth of other bacteria with a bactericidal or bacteriostatic mode of action. many lactic acid bacteria (lab) produce a high diversity of different bacteriocins. bacteriocinogenic lab are generally recognised as safe (gras) and useful to control the frequent development of pathogens and spoilage microorganisms. for this reason they are commonly used as starter cultures in food fermentations. in this study, the authors ...201627800430
enoyl-acyl carrier protein reductase i (fabi) is essential for the intracellular growth of listeria monocytogenes.enoyl-acyl carrier protein reductase catalyzes the last step in each elongation cycle of type ii bacterial fatty acid synthesis and is a key regulatory protein in bacterial fatty acid synthesis. genes of the facultative intracellular pathogen listeria monocytogenes encode two functional enoyl-acyl carrier protein isoforms based on their ability to complement the temperature-sensitive growth phenotype of escherichia coli strain jp1111 [fabi(ts)]. the fabi isoform was inactivated by the fabi selec ...201627736774
the scavenger receptor ssc5d physically interacts with bacteria through the srcr-containing n-terminal domain.the scavenger receptor cysteine-rich (srcr) family comprises a group of membrane-attached or secreted proteins that contain one or more modules/domains structurally similar to the membrane distal domain of type i macrophage scavenger receptor. although no all-inclusive biological function has been ascribed to the srcr family, some of these receptors have been shown to recognize pathogen-associated molecular patterns (pamp) of bacteria, fungi, or other microbes. ssc5d is a recently described solu ...201627790215
methodology for identification of pore forming antimicrobial peptides from soy protein subunits β-conglycinin and glycinin.antimicrobial peptides (amps) inactivate microbial cells through pore formation in cell membrane. because of their different mode of action compared to antibiotics, amps can be effectively used to combat drug resistant bacteria in human health. amps can also be used to replace antibiotics in animal feed and immobilized on food packaging films. in this research, we developed a methodology based on mechanistic evaluation of peptide-lipid bilayer interaction to identify amps from soy protein. produ ...201627612614
in vitro activity of plant extracts against biofilm-producing food-related bacteria.the identification of effective antimicrobial agents also active on biofilms is a topic of crucial importance in food and industrial environment. for that purpose methanol extracts of turkish plants, ficus carica l., juglans regia l., olea europaea l., punica granatum l. and rhus coriaria l., were investigated. among the extracts, p. granatum l. and r. coriaria l. showed the best antibacterial activity with minimum inhibitory concentrations (mic) of 78-625μg/ml for listeria monocytogenes and sta ...201627591384
a counterselection method for lactococcus lactis genome editing based on class iia bacteriocin sensitivity.in this paper, we present a new counterselection method for deleting fragments from lactococcus lactis chromosome. the method uses a non-replicating plasmid vector, which integrates into the chromosome and makes the cell sensitive to bacteriocins. the integration vector carries puc ori functional in escherichia coli but not in l. lactis, an erythromycin resistance gene for selecting single crossover integrants, and two fragments from l. lactis chromosome for homologous recombinations. in additio ...201627654656
reduced susceptibilities to biocides and resistance to antibiotics in food-associated bacteria following exposure to quaternary ammonium compounds.our aim was to assess the effects of step-wise exposure to didecyl dimethyl ammonium chloride (ddac) on the antimicrobial (antibiotics and biocides) susceptibilities of food-associated bacterial strains.201627481186
microfluidic cantilever detects bacteria and measures their susceptibility to antibiotics in small confined volumes.in the fight against drug-resistant bacteria, accurate and high-throughput detection is essential. here, a bimaterial microcantilever with an embedded microfluidic channel with internal surfaces chemically or physically functionalized with receptors selectively captures the bacteria passing through the channel. bacterial adsorption inside the cantilever results in changes in the resonance frequency (mass) and cantilever deflection (adsorption stress). the excitation of trapped bacteria using inf ...201627698375
identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains.this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ...201627625647
comprehensive evaluation and implementation of improvement actions in butcher shops.foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. a comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or ...201627618439
presence of escherichia coli o157, salmonella spp., and listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied.italy is one of the main producers and exporters of cheese made from unpasteurized sheep milk. since raw milk and its derived products are known sources of human infections, cheese produced from raw sheep milk could pose a microbiological threat to public health. hence, the objectives of the study were: to characterize the potential risk of the presence of pathogens escherichia coli o157, listeria monocytogenes, and salmonella in raw ovine milk destined for cheese production obtained from all th ...201627607835
development of a sandwich elisa for ehec o157:h7 intimin γ1.enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ...201627603508
characterization of egg white antibacterial properties during the first half of incubation: a comparative study between embryonated and unfertilized eggs.egg white is an important contributor to the protection of eggs against bacterial contaminations during the first half of incubation (day zero to 12), prior to the egg white transfer into the amniotic fluid to be orally absorbed by the embryo. this protective system relies on an arsenal of antimicrobial proteins and on intrinsic physicochemical properties that are generally unfavorable for bacterial multiplication and dissemination. some changes in these parameters can be observed in egg white d ...201627601682
do bacterial cell numbers follow a theoretical poisson distribution? comparison of experimentally obtained numbers of single cells with random number generation via computer simulation.we investigated a bacterial sample preparation procedure for single-cell studies. in the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical poisson distribution. four serotypes of salmonella enterica, three serotypes of enterohaemorrhagic escherichia coli and one serotype of listeria monocytogenes were used as sample bacteria. an inoculum of each serotype was prepared via a 10-fold dilution series to obtain bacterial cell counts with me ...201627554145
modeling optimal process conditions for uv-heat inactivation of foodborne pathogens in liquid foods.the combination of ultraviolet radiation and heat (uv-h treatment) has been demonstrated as a promising strategy to overcome the limited uv germicidal effect in fruit juices. nonetheless, there are so far no data regarding the efficacy of the combined process for the inactivation of bacterial foodborne pathogens in other liquid foods with different ph and composition. in this investigation, the optimum uv-h processing conditions for the inactivation of escherichia coli, salmonella typhimurium, l ...201627554141
rapid colorimetric sensing platform for the detection of listeria monocytogenes foodborne pathogen.listeria monocytogenes is a serious cause of human foodborne infections worldwide, which needs spending billions of dollars for inspection of bacterial contamination in food every year. therefore, there is an urgent need for rapid, in-field and cost effective detection techniques. in this study, rapid, low-cost and simple colorimetric assay was developed using magnetic nanoparticles for the detection of listeria bacteria. the protease from the listeria bacteria was detected using d-amino acid su ...201627543841
chitosan grafted monomethyl fumaric acid as a potential food preservative.the present study aims at in vitro antibacterial and antioxidant activity evaluation of chitosan modified with monomethyl fumaric acid (mfa) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc) as mediator. three different kinds of chitosan derivatives ch-ds-1,ch-ds-2 and ch-ds-3 were synthesized by feeding different concentration of mfa. the chemical structures of resulting materials were characterized by (1)h nmr, (13)c nmr, hr-xrd, ft-ir and tnbs assay. the results showed that ch-ds-1, ...201627516253
preparation of pectin/silver nanoparticles composite films with uv-light barrier and properties.silver nanoparticles (agnps) was synthesized by a green method using an aqueous extract of caesalpinia mimosoides lamk (cmle) as reducing and stabilizing agents, and they were used for the preparation of pectin-based antimicrobial composite films. the agnps were spherical in shape with the size in the range of 20-80nm and showed the absorption peak around 500nm. the pectin/agnps composite film exhibited characteristic absorption peak of agnps at 480nm. the surface color and light transmittance o ...201627492557
biochemical properties and mechanism of action of enterocin ld3 purified from enterococcus hirae ld3.enterocin ld3 was purified using activity-guided multistep chromatography techniques such as cation-exchange and gel-filtration chromatography. the preparation's purity was tested using reverse-phase ultra-performance liquid chromatography. the specific activity was tested to be 187.5 au µg(-1) with 13-fold purification. purified enterocin ld3 was heat stable up to 121 °c (at 15 psi pressure) and ph 2-6. the activity was lost in the presence of papain, reduced by proteinase k, pepsin and trypsin ...201627145777
enterococcus mundtii st4sa and lactobacillus plantarum 423 excludes listeria monocytogenes from the git, as shown by bioluminescent studies in mice.listeria monocytogenes is an opportunistic food-borne pathogen and is life-threatening to individuals with a weakened immune system. the aim of this study was to determine if lactobacillus plantarum 423 and enterococcus mundtii st4sa could prevent colonisation of l. monocytogenes in the gastro-intestinal tract (git). mice were gavaged with l. plantarum 423, e. mundtii st4sa, and a combination of the two strains, for 6 consecutive days and orally infected with a bioluminescent strain of l. monocy ...201626689230
evaluation of electrolytically-generated hypochlorous acid ('electrolyzed water') for sanitation of meat and meat-contact surfaces.'electrolyzed water' generators are readily available in the food industry as a renewable source of hypochlorous acid that eliminates the need for workers to handle hazardous hypochlorite concentrates. we applied electrolyzed water (ew) directly to multi-strain cocktails of listeria monocytogenes, e. coli o157:h7, and salmonella sp. at 250 ppm free available chlorine (fac) and achieved greater than 6-log reductions in 2 min. lower ew values were examined as antimicrobial interventions for fresh ...201628231137
purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris.malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ...201625837975
chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition.essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ...201628375110
experience with rapid microarray-based diagnostic technology and antimicrobial stewardship for patients with gram-positive bacteremia.objective to describe the impact of rapid diagnostic microarray technology and antimicrobial stewardship for patients with gram-positive blood cultures. design retrospective pre-intervention/post-intervention study. setting a 1,200-bed academic medical center. patients inpatients with blood cultures positive for staphylococcus aureus, enterococcus faecalis, e. faecium, streptococcus pneumoniae, s. pyogenes, s. agalactiae, s. anginosus, streptococcus spp., and listeria monocytogenes during the 6 ...201627767002
development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging.consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ...201627455176
antimicrobial and physicochemical characterization of biodegradable, nitric oxide-releasing nanocellulose-chitosan packaging membranes.biodegradable composite membranes with antimicrobial properties consisting of nanocellulose fibrils (cnfs), chitosan, and s-nitroso-n-acetyl-d-penicillamine (snap) were developed and tested for food packaging applications. as a nitric oxide donor, snap was encapsulated into completely dispersed chitosan in 100 ml of 0.1 n acetic acid and was thoroughly mixed with cnfs to produce a composite membrane. the fabricated membranes had a uniform dispersion of chitosan and snap within the cnfs, which wa ...201627258235
probiotic properties of lactic acid bacteria isolated from human milk.the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.201627159339
spatial organization of cell wall-anchored proteins at the surface of gram-positive bacteria.bacterial surface proteins constitute an amazing repertoire of molecules with important functions such as adherence, invasion, signalling and interaction with the host immune system or environment. in gram-positive bacteria, many surface proteins of the "lpxtg" family are anchored to the peptidoglycan (pg) by an enzyme named sortase. while this anchoring mechanism has been clearly deciphered, less is known about the spatial organization of cell wall-anchored proteins in the bacterial envelope. h ...201627025379
screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species.in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ...201626907753
tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m).tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ...201626682961
multicenter evaluation of biofire filmarray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens.rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. comprehensive testing of cerebrospinal fluid (csf) often includes gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. these methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. the filmarray meningitis/encephalitis (me) panel is a multiplexed in vitro di ...201627335149
a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens.galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ...201626615008
evaluation of the antioxidative, antibacterial, and anti-inflammatory effects of the aloe fermentation supernatant containing lactobacillus plantarum hm218749.1.little work is done to develop aloe vera (av) using probiotics. to explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of aloe fermentation supernatant were evaluated in vitro. our results indicated that the aloe fermentation supernatant fermented by lactobacillus plantarum hm218749.1 had very strong scavenging capacities of the dpph (86%), o2 (•-) (85%), (•)oh (76%), and fe(2+) chelation (82%) and reducing powers (242.5 mg/l), and the inh ...201627493450
a novel enterocin t1 with anti-pseudomonas activity produced by enterococcus faecium t1 from chinese tibet cheese.an enterocin-producing enterococcus faecium t1 was isolated from chinese tibet cheese. the enterocin was purified by sp-sepharose and reversed phase hplc. it was identified as unique from other reported bacteriocins based on molecular weight (4629 da) and amino acid compositions; therefore it was subsequently named enterocin t1. enterocin t1 was stable at 80-100 °c and over a wide ph range, ph 3.0-10.0. protease sensitivity was observed to trypsin, pepsin, papain, proteinase k, and pronase e. im ...201626745981
antibiotic susceptibility, antibacterial activity and characterisation of enterococcus faecium strains isolated from breast milk.enterococci, which have useful biotechnological applications, produce bacteriocins, including those that exert anti-listerial activity. the present study aimed to determine the antibiotic susceptibility patterns and antimicrobial activity of enterococcus faecium strains isolated from human breast milk. the strains were identified using carbohydrate fermentation tests and ribotyping. subsequently, the antibacterial activity of the isolates was investigated, and the quantities of lactic acid and h ...201627602088
purification of a novel bacteriocin-like inhibitory substance produced by enterococcus faecium icis 8 and characterization of its mode of action.the aim of this work was to purify and characterize a bacteriocin-like antimicrobial substance produced by an antagonistic active strain of enterococcus faecium.201627575914
core-shell nanorod columnar array combined with gold nanoplate-nanosphere assemblies enable powerful in situ sers detection of bacteria.development of a label-free ultrasensitive nanosensor for detection of bacteria is presented. sensitive assay for gram-positive bacteria was achieved via electrostatic attraction-guided plasmonic bifacial superstructure/bacteria/columnar array assembled in one step. dynamic optical hotspots were formed in the hybridized nanoassembly under wet-dry critical state amplifying efficiently the weak vibrational modes of three representative food-borne gram-positive bacteria, that is, staphylococcus xyl ...201627574829
pediocin sa-1: a selective bacteriocin for controlling listeria monocytogenes in maize silages.in this study, we assessed the potential as silage additive of a bacteriocin produced by pediococcus acidilactici northern regional research laboratory (nrrl) b-5627 (pediocin sa-1). maize was inoculated either with a bacterial starter alone (i) or in combination with the bacteriocin (ip), and untreated silage served as control. we monitored the products of fermentation (ethanol, and lactic and acetic acids), the microbial population, and the presence of the indicator strain listeria monocytogen ...201627522409
evaluation of technological properties of enterococcus faecium cect 8849, a strain isolated from human milk, for the dairy industry.in this work, a variety of biochemical properties of enterococcus faecium cect 8849, which had been isolated from breast milk, were analyzed. its acidifying capacity and proteolytic activity were low but, in contrast, remarkable peptidase and esterase activities were observed. ethanol and 3-hydroxy-2-butanone were the most abundant volatile compounds found in experimental model cheese manufactured with e. faecium cect 8849. this strain inhibited the growth of several listeria monocytogenes and l ...201627216614
inactivation of salmonella, listeria monocytogenes and enterococcus faecium nrrl b-2354 in a selection of low moisture foods.the aims of this study were to obtain data on survival and heat resistance of cocktails of salmonella, listeria monocytogenes and the surrogate enterococcus faecium (nrrl b-2354) in four low moisture foods (confectionery formulation, chicken meat powder, pet food and savoury seasoning) during storage before processing. inoculated samples were stored at 16°c and cell viability examined at day 0, 3, 7 and 21. at each time point, the heat resistance at 80°c was determined. the purpose was to determ ...201627174678
analysis of the impact of rosuvastatin on bacterial mevalonate production using a uplc-mass spectrometry approach.statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme a reductase (hmg-r) which produces mevalonate (mval), a key substrate for cholesterol biosynthesis. some important microbial species also express an isoform of hmg-r; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify mval in complex microbial environments. the objective o ...201626960292
transfer of antibiotic resistance from enterococcus faecium of fermented meat origin to listeria monocytogenes and listeria innocua.listeria monocytogenes is an important foodborne pathogen that can cause infection in children, pregnant women, the immunocompromised and the elderly. antibiotic resistance in this species would represent a significant public health problem since the organism has a high fatality/case ratio and resistance may contribute to failure of therapeutic treatment. this study was designed to explore whether the in vitro transferability of antibiotic resistance from enterococci to listeria spp. could occur ...201626854329
development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese.dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ...201627840628
persistence and potential viable but non-culturable state of pathogenic bacteria during storage of digestates from agricultural biogas plants.despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (salmonella enterica derby, campylobacter coli and listeria monocytogenes) in digestates from farms, stored for later land spreading. nine samples, including raw digestates, liq ...201627695451
fate of viable but non-culturable listeria monocytogenes in pig manure microcosms.the fate of two strains of listeria monocytogenes and their ability to become viable but non-culturable (vbnc) was investigated in microcosms containing piggery effluents (two raw manures and two biologically treated manures) stored for 2 months at 8 and 20°c. levels of l. monocytogenes were estimated using the culture method, qpcr, and propidium monoazide treatment combined with qpcr (qpcrpma). the chemical composition and the microbial community structure of the manures were also analyzed. the s ...201626973623
cell-based screen identifies human interferon-stimulated regulators of listeria monocytogenes infection.the type i interferon (ifn) activated transcriptional response is a critical antiviral defense mechanism, yet its role in bacterial pathogenesis remains less well characterized. using an intracellular pathogen listeria monocytogenes (lm) as a model bacterial pathogen, we sought to identify the roles of individual interferon-stimulated genes (isgs) in context of bacterial infection. previously, ifn has been implicated in both restricting and promoting lm growth and immune stimulatory functions in ...201628002492
the listeria monocytogenes pasta kinase prka and its substrate yvck are required for cell wall homeostasis, metabolism, and virulence.obstacles to bacterial survival and replication in the cytosol of host cells, and the mechanisms used by bacterial pathogens to adapt to this niche are not well understood. listeria monocytogenes is a well-studied gram-positive foodborne pathogen that has evolved to invade and replicate within the host cell cytosol; yet the mechanisms by which it senses and responds to stress to survive in the cytosol are largely unknown. to assess the role of the l. monocytogenes penicillin-binding-protein and ...201627806131
temperature-dependent gentamicin resistance of francisella tularensis is mediated by uptake modulation.gentamicin (gm) is an aminoglycoside commonly used to treat bacterial infections such as tularemia - the disease caused by francisella tularensis. in addition to being pathogenic, f. tularensis is found in environmental niches such as soil where this bacterium likely encounters gm producers (micromonospora sp.). here we show that f. tularensis exhibits increased resistance to gm at ambient temperature (26°c) compared to mammalian body temperature (37°c). to evaluate whether f. tularensis was les ...201626858709
listeria monocytogenes and the inflammasome: from cytosolic bacteriolysis to tumor immunotherapy.inflammasomes are cytosolic innate immune surveillance systems that recognize a variety of danger signals, including those from pathogens. listeria monocytogenes is a gram-positive intracellular bacterium evolved to live within the harsh environment of the host cytosol. further, l. monocytogenes can activate a robust cell-mediated immune response that is being harnessed as an immunotherapeutic platform. access to the cytosol is critical for both causing disease and inducing a protective immune r ...201627460808
chemistry and molecular dynamics simulations of heme b-hemq and coproheme-hemq.recently, a novel pathway for heme b biosynthesis in gram-positive bacteria has been proposed. the final poorly understood step is catalyzed by an enzyme called hemq and includes two decarboxylation reactions leading from coproheme to heme b. coproheme has been suggested to act as both substrate and redox active cofactor in this reaction. in the study presented here, we focus on hemqs from listeria monocytogenes (lmhemq) and staphylococcus aureus (sahemq) recombinantly produced as apoproteins in ...201627599156
structural basis of rifampin inactivation by rifampin phosphotransferase.rifampin (rif) is a first-line drug used for the treatment of tuberculosis and other bacterial infections. various rif resistance mechanisms have been reported, and recently an rif-inactivation enzyme, rif phosphotransferase (rph), was reported to phosphorylate rif at its c21 hydroxyl at the cost of atp. however, the underlying molecular mechanism remained unknown. here, we solve the structures of rph from listeria monocytogenes (lmrph) in different conformations. lmrph comprises three domains: ...201627001859
inactivation of salmonella spp. and listeria spp. by palmitic, stearic, and oleic acid sophorolipids and thiamine dilauryl sulfate.food contaminated with human pathogens, such as salmonella spp. and listeria monocytogenes, frequently causes outbreaks of foodborne illness. consumer concern over the use of synthesized antimicrobials to enhance microbial food safety has led to a search of natural alternatives. the objectives of this study were to evaluate the antimicrobial activity of various types of sophorolipids (sls) and thiamine dilauryl sulfate (tds) against pathogenic salmonella spp. and listeria spp. both free and lact ...201628066390
listeria monocytogenes dna glycosylase adlp affects flagellar motility, biofilm formation, virulence, and stress responses.the temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogen listeria monocytogenes to respond to a changing environment. in this study, a genetic determinant, l. monocytogenes f2365_0220 (lmof2365_0220), encoding a putative protein that is structurally similar to the bacillus cereus alkyl base dna glycosylase (alkd), was identified. this determinant was involved in the transcriptional repression of flagellar motility genes and was named adlp ...201627316964
matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) can precisely discriminate the lineages of listeria monocytogenes and species of listeria.the genetic lineages of listeria monocytogenes and other species of the genus listeria are correlated with pathogenesis in humans. although matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of listeria species and lineages remains a crucial issue in clinical settings and for food safety. in this study, we constructed an accurate and reliable ms databa ...201627442502
thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens.due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ...201627486447
sublethal concentrations of antibiotics cause shift to anaerobic metabolism in listeria monocytogenes and induce phenotypes linked to antibiotic tolerance.the human pathogenic bacterium listeria monocytogenes is exposed to antibiotics both during clinical treatment and in its saprophytic lifestyle. as one of the keys to successful treatment is continued antibiotic sensitivity, the purpose of this study was to determine if exposure to sublethal antibiotic concentrations would affect the bacterial physiology and induce antibiotic tolerance. transcriptomic analyses demonstrated that each of the four antibiotics tested caused an antibiotic-specific ge ...201627462313
different transcriptional responses from slow and fast growth rate strains of listeria monocytogenes adapted to low temperature.listeria monocytogenes has become one of the principal foodborne pathogens worldwide. the capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of l. monocytogenes with different growth capacities at low temperatures. we determined the growth rate at 8°c of 110 strains of l. monocytogenes isolated from different food matrices. we identified a group of slow and fast strains according to thei ...201626973610
effects of tween 80 on growth and biofilm formation in laboratory media.tween 80 is a widely used non-ionic emulsifier that is added to cosmetics, pharmaceuticals, and foods. because of its widespread use we need to understand how it affects bacteria on our skin, in our gut, and in food products. the aim of this study is to investigate how tween 80 affects the growth and antimicrobial susceptibility of staphylococcus aureus, listeria monocytogenes, and pseudomonas fluorescens, which are common causes of spoilage and foodborne illnesses. addition of 0.1% tween 80 to ...201627920774
increased biomass production by mesophilic food-associated bacteria through lowering the growth temperature from 30°c to 10°c.five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species escherichia coli, listeria monocytogenes, staphylococcus xylosus, bacillus cereus, pedobacter nutrimenti, and pedobacter panaciterrae were tested for the effect of growth temperature (30°c and 10°c) on biomass formation. growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and define ...201627084015
study of the population dynamics of listeria monocytogenes and pseudomonas fluorescens in buffalo mozzarella by means of challenge testing.campania's buffalo mozzarella is a greatly appreciated cheese in italy and worldwide. from a microbiological standpoint, it is a highly perishable food and potentially at risk of contamination by pathogens such as listeria monocytogenes (l. monocytogenes). the present paper reports the results of a challenge test carried out to assess the population dynamics of l. monocytogenes, alone and in presence of pseudomonas fluorescens (p. fluorescens), in buffalo mozzarella. for this purpose buffalo moz ...201627853707
use of metagenomic shotgun sequencing technology to detect foodborne pathogens within the microbiome of the beef production chain.foodborne illnesses associated with pathogenic bacteria are a global public health and economic challenge. the diversity of microorganisms (pathogenic and nonpathogenic) that exists within the food and meat industries complicates efforts to understand pathogen ecology. further, little is known about the interaction of pathogens within the microbiome throughout the meat production chain. here, a metagenomic approach and shotgun sequencing technology were used as tools to detect pathogenic bacteri ...201626873315
listeria monocytogenes impact on mature or old pseudomonas fluorescens biofilms during growth at 4 and 20°c.changes in spatial organization, as observed by confocal laser scanning microscopy (clsm), viable cell content, biovolume, and substratum surface coverage of the biofilms formed on glass by pseudomonas fluorescens resulting from co-culture with listeria monocytogenes, were examined. two strains of l. monocytogenes, two culture temperatures and two biofilm developmental stages were investigated. both l. monocytogenes strains, a persistently sampled isolate (collected repeatedly along 3 years from ...201626913024
evaluation of the performance of quantitative detection of the listeria monocytogenes prfa locus with droplet digital pcr.fast and reliable pathogen detection is an important issue for human health. since conventional microbiological methods are rather slow, there is growing interest in detection and quantification using molecular methods. the droplet digital polymerase chain reaction (ddpcr) is a relatively new pcr method for absolute and accurate quantification without external standards. using the listeria monocytogenes specific prfa assay, we focused on the questions of whether the assay was directly transferab ...201627558101
exploring the diversity and antimicrobial potential of marine actinobacteria from the comau fjord in northern patagonia, chile.bioprospecting natural products in marine bacteria from fjord environments are attractive due to their unique geographical features. although, actinobacteria are well known for producing a myriad of bioactive compounds, investigations regarding fjord-derived marine actinobacteria are scarce. in this study, the diversity and biotechnological potential of actinobacteria isolated from marine sediments within the comau fjord, in northern chilean patagonia, were assessed by culture-based approaches. ...201627486455
structural basis for glutathione-mediated activation of the virulence regulatory protein prfa in listeria.infection by the human bacterial pathogen listeria monocytogenes is mainly controlled by the positive regulatory factor a (prfa), a member of the crp/fnr family of transcriptional activators. published data suggest that prfa requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (gsh) could fulfill this function. here we report the crystal structures of prfa in complex with gsh and in complex with gsh and its cognate dna, the hly operator prfa box mot ...201627930316
chemical defence in a millipede: evaluation and characterization of antimicrobial activity of the defensive secretion from pachyiulus hungaricus (karsch, 1881) (diplopoda, julida, julidae).the chemical defence of the millipede pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. in total, independently of sex, 44 compounds were identified. all compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. the relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. the two dominant quinones in b ...201627907048
licheniocin 50.2 and bacteriocins from lactococcus lactis subsp. lactis biovar. diacetylactis bgbu1-4 inhibit biofilms of coagulase negative staphylococci and listeria monocytogenes clinical isolates.coagulase negative staphylococci (cons) and listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. the aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of cons and l. monocytogenes genital isolates.201627930711
comparison of surface proteomes of adherence variants of listeria monocytogenes using lc-ms/ms for identification of potential surface adhesins.the ability of listeria monocytogenes to adhere and form biofilms leads to persistence in food processing plants and food-associated listeriosis. the role of specific surface proteins as adhesins to attach listeria cells to various contact surfaces has not been well characterized to date. in prior research comparing different methods for surface protein extraction, the ghost urea method revealed cleaner protein content as verified by the least cytoplasmic protein detected in surface extracts usi ...201627196934
antimicrobial activity of croton macrostachyus stem bark extracts against several human pathogenic bacteria.in kenya, leaves and roots from croton macrostachyus are used as a traditional medicine for infectious diseases such as typhoid and measles, but reports on possible antimicrobial activity of stem bark do not exist. in this study, the antibacterial and antifungal effects of methanol, ethyl acetate and butanol extracts, and purified lupeol of c. macrostachyus stem bark were determined against important human gram-negative pathogens escherichia coli, salmonella typhi, klebsiella pneumoniae, and ent ...201627293897
septins and bacterial infection.septins, a unique cytoskeletal component associated with cellular membranes, are increasingly recognized as having important roles in host defense against bacterial infection. a role for septins during invasion of listeria monocytogenes into host cells was first proposed in 2002. since then, work has shown that septins assemble in response to a wide variety of invasive bacterial pathogens, and septin assemblies can have different roles during the bacterial infection process. here we review the i ...201627891501
modulation of stat-1 in human macrophages infected with different species of intracellular pathogenic bacteria.the infection of human macrophages by pathogenic bacteria induces different signaling pathways depending on the type of cellular receptors involved in the microorganism entry and on their mechanism(s) of survival and replication in the host cell. it was reported that stat proteins play an important role in this process. in the present study, we investigate the changes in stat-1 activation (phosphorylation in p-tyr701) after uptake of two gram-positive (listeria monocytogenes and staphylococcus a ...201627437406
spovg is a conserved rna-binding protein that regulates listeria monocytogenes lysozyme resistance, virulence, and swarming motility.in this study, we sought to characterize the targets of the abundantlisteria monocytogenesnoncoding rna rli31, which is required forl. monocytogeneslysozyme resistance and pathogenesis. whole-genome sequencing of lysozyme-resistant suppressor strains identified loss-of-expression mutations in the promoter ofspovg, and deletion ofspovgrescued lysozyme sensitivity and attenuationin vivoof therli31mutant. spovg was demonstrated to be an rna-binding protein that interacted with rli31in vitro.the rel ...201627048798
response to interferons and antibacterial innate immunity in the absence of tyrosine-phosphorylated stat1.signal transducer and activator of transcription 1 (stat1) plays a pivotal role in the innate immune system by directing the transcriptional response to interferons (ifns). stat1 is activated by janus kinase (jak)-mediated phosphorylation of y701. to determine whether stat1 contributes to cellular responses without this phosphorylation event, we generated mice with y701 mutated to a phenylalanine (stat1(y701f)). we show that heterozygous mice do not exhibit a dominant-negative phenotype. homozyg ...201626882544
molecular analysis of dominant species in listeria monocytogenes-positive biofilms in the drains of food processing facilities.listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. in this study, we investigated l. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species of the biofilms were identified to determine the properties of the microbiological background. for this purpose, the iso 11290 method was used for the detection and isolation of l. m ...201626658820
screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang).the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ...201626718466
antimicrobial activity of the pygidial gland secretion of three ground beetle species (insecta: coleoptera: carabidae).the antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, carabus ullrichii, c. coriaceus, and abax parallelepipedus, have been tested. microdilution method was applied for detection of minimal inhibitory concentrations (mics), minimal bactericidal concentrations (mbcs), and minimal fungicidal concentrations (mfcs). additionally, morpho-histology of the pygidial glands is investigated. we have tested 16 laboratory and clinical strain ...201627000261
extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes.bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ...201627840979
antimicrobial activity of kefir against various food pathogens and spoilage bacteria.kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ...201628115890
risk assessment in the recovery of food for social solidarity purposes: preliminary data.the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ...201628058252
differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region.the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ...201627853715
evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life.ricotta salata cheese is a salted variety of ricotta traditionally made in sardinia (italy) from the whey remaining after the production of pecorino romano protected designation of origin or other sheep milk cheeses. ricotta salata cheese is very critical for the possible growth of pathogenic and spoilage microorganisms. sporadic cases of listeriosis associated with ricotta salata cheese have been reported over recent years. the objective of the present study was to assess the evolution of spoil ...201627800440
isolation and characterization of a broad spectrum bacteriocin from bacillus amyloliquefaciens rx7.we isolated a bacillus strain, rx7, with inhibitory activity against listeria monocytogenes from soil and identified it as bacillus amyloliquefaciens based on 16s rrna gene sequencing. the inhibitory activity was stable over a wide range of ph and was fully retained after 30 min at 80°c, after which it decreased gradually at higher temperatures. the activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-k, and trypsin, indicating its proteinaceous nature. this bacteriocin ...201627239477
cyclic dipeptide cyclo(l-leucyl-l-prolyl) from marine bacillus amyloliquefaciens mitigates biofilm formation and virulence in listeria monocytogenes.this study was intentionally focused on cyclo(l-leucyl-l-prolyl) (clp), a cyclic dipeptide with myriad pharmaceutical significance, to explore its antivirulence efficacy against the predominant foodborne pathogen,listeria monocytogenes(lm). minimum inhibitory concentration (mic) of clp against lm atcc 19111 was found to be 512 μg ml(-1) clp at sub-mics (64 128, 256 μg ml(-1)) demonstrated a profound non-bactericidal dose-dependent antibiofilm efficacy (on polystyrene and glass) against lm, which ...201626945590
fapr: from control of membrane lipid homeostasis to a biotechnological tool.phospholipids and fatty acids are not only one of the major components of cell membranes but also important metabolic intermediates in bacteria. since the fatty acid biosynthetic pathway is essential and energetically expensive, organisms have developed a diversity of homeostatic mechanisms to fine-tune the concentration of lipids at particular levels. fapr is the first global regulator of lipid synthesis discovered in bacteria and is largely conserved in gram-positive organisms including import ...201627766255
a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute.in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ...201627874136
proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules.the chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, tenp, ovalbu ...201627294500
bacterial diversity of floor drain biofilms and drain waters in a listeria monocytogenes contaminated food processing environment.sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring listeria monocytogenes in food processing facilities. microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid efflue ...201626881738
molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea).the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ...201628105799
effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis).the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ...201627208583
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