Publications

extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes.	bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ...	2016	27840979
screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang).	the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ...	2016	26718466
isolation and characterization of a broad spectrum bacteriocin from bacillus amyloliquefaciens rx7.	we isolated a bacillus strain, rx7, with inhibitory activity against listeria monocytogenes from soil and identified it as bacillus amyloliquefaciens based on 16s rrna gene sequencing. the inhibitory activity was stable over a wide range of ph and was fully retained after 30 min at 80°c, after which it decreased gradually at higher temperatures. the activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-k, and trypsin, indicating its proteinaceous nature. this bacteriocin ...	2016	27239477
cyclic dipeptide cyclo(l-leucyl-l-prolyl) from marine bacillus amyloliquefaciens mitigates biofilm formation and virulence in listeria monocytogenes.	this study was intentionally focused on cyclo(l-leucyl-l-prolyl) (clp), a cyclic dipeptide with myriad pharmaceutical significance, to explore its antivirulence efficacy against the predominant foodborne pathogen,listeria monocytogenes(lm). minimum inhibitory concentration (mic) of clp against lm atcc 19111 was found to be 512 μg ml(-1) clp at sub-mics (64 128, 256 μg ml(-1)) demonstrated a profound non-bactericidal dose-dependent antibiofilm efficacy (on polystyrene and glass) against lm, which ...	2016	26945590
a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute.	in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ...	2016	27874136
antimicrobial activity of the pygidial gland secretion of three ground beetle species (insecta: coleoptera: carabidae).	the antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, carabus ullrichii, c. coriaceus, and abax parallelepipedus, have been tested. microdilution method was applied for detection of minimal inhibitory concentrations (mics), minimal bactericidal concentrations (mbcs), and minimal fungicidal concentrations (mfcs). additionally, morpho-histology of the pygidial glands is investigated. we have tested 16 laboratory and clinical strain ...	2016	27000261
proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules.	the chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, tenp, ovalbu ...	2016	27294500
bacterial diversity of floor drain biofilms and drain waters in a listeria monocytogenes contaminated food processing environment.	sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring listeria monocytogenes in food processing facilities. microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid efflue ...	2016	26881738
molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea).	the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ...	2016	28105799
effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis).	the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ...	2016	27208583
molecular analysis of dominant species in listeria monocytogenes-positive biofilms in the drains of food processing facilities.	listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. in this study, we investigated l. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species of the biofilms were identified to determine the properties of the microbiological background. for this purpose, the iso 11290 method was used for the detection and isolation of l. m ...	2016	26658820
fapr: from control of membrane lipid homeostasis to a biotechnological tool.	phospholipids and fatty acids are not only one of the major components of cell membranes but also important metabolic intermediates in bacteria. since the fatty acid biosynthetic pathway is essential and energetically expensive, organisms have developed a diversity of homeostatic mechanisms to fine-tune the concentration of lipids at particular levels. fapr is the first global regulator of lipid synthesis discovered in bacteria and is largely conserved in gram-positive organisms including import ...	2016	27766255
antimicrobial activity of kefir against various food pathogens and spoilage bacteria.	kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ...	2016	28115890
the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria.	goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ...	2016	27698594
simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods.	capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ...	2016	27494415
bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico.	currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ...	2016	27407294
biological activities of aerial parts extracts of euphorbia characias.	the aim of the present study was to evaluate antioxidant, antimicrobial, anti-hiv, and cholinesterase inhibitory activities of aqueous and alcoholic extracts from leaves, stems, and flowers of euphorbia characias. the extracts showed a high antioxidant activity and were a good source of total polyphenols and flavonoids. ethanolic extracts from leaves and flowers displayed the highest inhibitory activity against acetylcholinesterase and butyrylcholinesterase, showing potential properties against ...	2016	27314007
a rapid multiplex real-time pcr high-resolution melt curve assay for the simultaneous detection of bacillus cereus, listeria monocytogenes, and staphylococcus aureus in food.	three important foodborne pathogens, bacillus cereus, listeria monocytogenes, and staphylococcus aureus, are of great concern for food safety. they may also coexist in food matrices and, in the case of b. cereus and s. aureus, the resulting illnesses can resemble each other owing to similar symptoms. therefore, their simultaneous detection may have advantages in terms of cost savings and rapidity. given this context, a rapid multiplex real-time pcr high-resolution melt curve assay for the simult ...	2016	27296430
assessment of the microbiological quality of meat pies from retail sale in england 2013.	outbreaks of foodborne illness caused by bacillus cereus and listeria monocytogenes in england associated with meat pie consumption were detected in 2012. to obtain baseline data for pies unrelated to outbreaks, 862 samples of ready-to-eat meat pies were collected at retail or from catering facilities in england in 2013 and examined to enumerate food-poisoning bacteria and indicator organisms using organization for standardization (iso) methods for listeria spp. including l. monocytogenes (iso 1 ...	2016	27296425
innovative bionanocomposite films of edible proteins containing liposome-encapsulated nisin and halloysite nanoclay.	films and coatings based on natural polymers have gained increased interest for food packaging applications. in this work, halloysite and phosphatidylcholine liposomes encapsulating nisin were used to develop nanocomposite films of gelatin and casein. liposomes prepared with either soybean lecithin or phospholipon(®) showed particle size ranging from 124 to 178nm and high entrapment efficiency (94-100%). considering their stability, phospholipon(®) liposomes with 1.0mg/ml nisin were selected for ...	2016	27289315
the impact of oxygen on bacterial enteric pathogens.	bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ...	2016	27261784
potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages.	this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ...	2016	27112426
chemical composition, antioxidant and antibacterial activities of essential oils from ferulago angulata.	ferulago angulata boiss. (apiaceae), a perennial aromatic herb, grows wild in iran. the aerial parts of f. angulata are used as a flavouring in foods, especially dairy foods by indigenous people in western and southwestern iran.	2016	27102982
recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph.	the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ...	2016	26976751
application of mathematical models for bacterial inactivation curves using hypericin-based photosensitization.	the aim of this study was to evaluate which of the three mathematical models (weibull, geeraerd and logistic) makes the best approximation for hypericin-based photosensitization bacterial inactivation.	2016	26972682
isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria.	a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ...	2016	26921428
influence of season and type of restaurants on sashimi microbiota.	in recent years, an increase in the consumption of japanese food in european countries has been verified, including in portugal. these specialities made with raw fish, typical japanese meals, have been prepared in typical and on non-typical restaurants, and represent a challenge to risk analysis on haccp plans. the aim of this study was to evaluate the influence of the type of restaurant, season and type of fish used on sashimi microbiota.	2016	26911490
understanding the antimicrobial activity behind thin- and thick-rolled copper plates.	the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ...	2016	26860943
evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk.	lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ...	2016	26805974
characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food.	a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ...	2016	26803269
antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens.	the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ...	2016	26775951
advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges.	the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ...	2016	26745757
safety of street-vended soy wara in nigeria.	soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ...	2016	26735046
inactivation of bacterial pathogens in yoba mutandabota, a dairy product fermented with the probiotic lactobacillus rhamnosus yoba.	mutandabota is a dairy product consumed as a major source of proteins and micronutrients in southern africa. in this study the microbial safety of traditional and a variant of mutandabota fermented with the probiotic lactobacillus rhamnosus yoba (yoba mutandabota) was investigated by challenging the products with five important food pathogens: listeria monocytogenes, salmonella spp., campylobacter jejuni, escherichia coli o157:h7 and bacillus cereus. pasteurized full-fat cow's milk was used for ...	2016	26490648
effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review.	according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ...	2016	25612827
risk assessment in the recovery of food for social solidarity purposes: preliminary data.	the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ...	2016	28058252
differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region.	the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ...	2016	27853715
evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life.	ricotta salata cheese is a salted variety of ricotta traditionally made in sardinia (italy) from the whey remaining after the production of pecorino romano protected designation of origin or other sheep milk cheeses. ricotta salata cheese is very critical for the possible growth of pathogenic and spoilage microorganisms. sporadic cases of listeriosis associated with ricotta salata cheese have been reported over recent years. the objective of the present study was to assess the evolution of spoil ...	2016	27800440
bacillus licheniformis contains two more perr-like proteins in addition to perr, fur, and zur orthologues.	the ferric uptake regulator (fur) family proteins include sensors of fe (fur), zn (zur), and peroxide (perr). among fur family proteins, fur and zur are ubiquitous in most prokaryotic organisms, whereas perr exists mainly in gram positive bacteria as a functional homologue of oxyr. gram positive bacteria such as bacillus subtilis, listeria monocytogenes and staphylococcus aureus encode three fur family proteins: fur, zur, and perr. in this study, we identified five fur family proteins from b. li ...	2016	27176811
comparative evaluation of the antimicrobial activity of 19 essential oils.	in our research on natural compounds efficient against human pathogen or opportunist microorganisms contracted by food or water, the antimicrobial activity of 19 essential oils (eos) was investigated against 11 bacterial species (6 gram positive, 5 gram negative) and 7 fungal species (2 dermatophytes, 1 mould, 4 yeasts) using microdilution assays. five essential oils were obtained from tunisian plants (eotun): artemisia herba-alba asso, juniperus phoenicea l., rosmarinus officinalis l., ruta gra ...	2016	26566647
purification and characterization of a novel anti-campylobacter bacteriocin produced by lactobacillus curvatus dn317.	the lactic acid bacteria (lab) microbiota of saudi chicken ceca was determined. from 60 samples, 204 isolates of lactic acid bacteria were obtained. three isolates produced antimicrobial activities against campylobacter jejuni, listeria monocytogenes, and bacillus subtilis. the isolate dn317, which had the highest activity against campylobacter jejuni atcc 33560, was identified as lactobacillus curvatus (genbank accession numbers: kx353849 and kx353850). full inhibitory activity was observed aft ...	2016	27812926
f-type bacteriocins of listeria monocytogenes: a new class of phage tail-like structures reveals broad parallel coevolution between tailed bacteriophages and high-molecular-weight bacteriocins.	listeria monocytogenes is a significant foodborne human pathogen that can cause severe disease in certain high-risk individuals. l. monocytogenes is known to produce high-molecular-weight, phage tail-like bacteriocins, or "monocins," upon induction of the sos system. in this work, we purified and characterized monocins and found them to be a new class of f-type bacteriocins. the l. monocytogenes monocin genetic locus was cloned and expressed in bacillus subtilis, producing specifically targeted ...	2016	27457717
subunit arrangement in gpsb, a regulator of cell wall biosynthesis.	gpsb, a key regulator of cell division in gram-positive bacteria, interacts with a key peptidoglycan synthase at the cell division septum, the penicillin binding protein pbp1 (a.k.a. pona). bacillus subtilis gpsb has been reported to interact with other components of the cell division machinery, including ezra, mrec, and prkc. in this study, we report an analysis of the arrangement of subunits in listeria monocytogenes gpsb by small-angle x-ray scattering. the resulting model has an elongated sh ...	2016	27257764
anti-biofilm and sporicidal activity of peptides based on wheat puroindoline and barley hordoindoline proteins.	the broad-spectrum activity of antimicrobial peptides (amps) and low probability of development of host resistance make them excellent candidates as novel bio-control agents. a number of amps are found to be cationic, and a small proportion of these are tryptophan-rich. the puroindolines (pin) are small, basic proteins found in wheat grains with proposed roles in biotic defence of seeds and seedlings. synthetic peptides based on their unique tryptophan-rich domain (trd) display antimicrobial pro ...	2016	27238815
arginine patch predicts the rna annealing activity of hfq from gram-negative and gram-positive bacteria.	the sm-protein hfq facilitates interactions between small non-coding rna (srna) and target mrnas. in enteric gram-negative bacteria, hfq is required for srna regulation, and hfq deletion results in stress intolerance and reduced virulence. by contrast, the role of hfq in gram-positive is less established and varies among species. the rna binding and rna annealing activity of hfq from escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus subtilis, and staphylococcus aureus we ...	2016	27049793
screening of probiotic activities of lactobacilli strains isolated from traditional tibetan qula, a raw yak milk cheese.	in this study, 69 lactobacilli isolated from tibetan qula, a raw yak milk cheese, were screened for their potential use as probiotics. the isolates were tested in terms of: their ability to survive at ph 2.0, ph 3.0, and in the presence of 0.3% bile salts; tolerance of simulated gastric and intestinal juices; antimicrobial activity; sensitivity against 11 specific antibiotics; and their cell surface hydrophobicity. the results show that out of the 69 strains, 29 strains (42%) had survival rates ...	2016	26954218
the listeria monocytogenes fur-regulated virulence protein frva is an fe(ii) efflux p1b4 -type atpase.	listeria monocytogenes frva (lmo0641) is critical for virulence in the mouse model and is an ortholog of the bacillus subtilis fur- and perr-regulated fe(ii) efflux p1b4 -type atpase pfet. previously, frva was suggested to protect against heme toxicity. here, we demonstrate that an frva mutant is sensitive to iron intoxication, but not to other metals. expression of frva is induced by high iron and this induction requires fur. frva functions in vitro as a divalent cation specific atpase most str ...	2016	26946370
an advanced bioinformatics approach for analyzing rna-seq data reveals sigma h-dependent regulation of competence genes in listeria monocytogenes.	alternative σ factors are important transcriptional regulators in bacteria. while σ(b) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen listeria monocytogenes, the function of σ(h) has not yet been well defined in listeria, even though σ(h) controls a large regulon in the closely related non-pathogenic bacillus subtilis.	2016	26880300
purification and characteristics of a novel bacteriocin produced by enterococcus faecalis l11 isolated from chinese traditional fermented cucumber.	to purify and characterize a novel bacteriocin with broad inhibitory spectrum produced by an isolate of enterococcus faecalis from chinese fermented cucumber.	2016	26875092
identification and characterization of holgh15: the holin of staphylococcus aureus bacteriophage gh15.	holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate and form lesions in the cytoplasmic membrane. the orf72 gene (also designated holgh15) derived from the genome of the staphylococcus aureus phage gh15 was predicted to encode a membrane protein. an analysis indicated that the protein encoded by holgh15 potentially consisted of two hydrophobic transmembrane helices. this protein exhibited the structural characteristics of class ii holins and ...	2016	26873847
comparison of antimicrobial activities of newly obtained low molecular weight scorpion chitosan and medium molecular weight commercial chitosan.	in this study the antimicrobial activity of low molecular weight (3.22 kda) chitosan, obtained for the first time from a species belonging to the scorpiones, was screened against nine pathogenic microorganisms (seven bacteria and two yeasts) and compared with that of medium molecular weight commercial chitosan (mmwcc). it was observed that the antimicrobial activity of the low molecular weight scorpion chitosan (lmwsc) was specific to bacterial species in general rather than gram-negative or gra ...	2016	26702952
zoonotic public health hazards in backyard chickens.	backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ...	2016	26752227
eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella.	listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ...	2016	27002648
purification of antilisterial peptide (subtilosin a) from novel bacillus tequilensis fr9 and demonstrate their pathogen invasion protection ability using human carcinoma cell line.	this study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (gallus gallus domesticus). fifty seven colonies were isolated and three isolates (fr4, fr9, and fr12) were selected and identified as lactobacillus gasseri fr4, bacillus tequilensis fr9, and l. animalis fr12 by 16s rrna sequencing. three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. especially, fr9 exh ...	2016	27990138
identification of coxiella burnetii cd8+ t-cell epitopes and delivery by attenuated listeria monocytogenes as a vaccine vector in a c57bl/6 mouse model.	coxiella burnetii is a gram-negative bacterium that causes acute and chronic q fever. because of the severe adverse effect of whole-cell vaccination, identification of immunodominant antigens of c. burnetii has become a major focus of q fever vaccine development. we hypothesized that secreted c. burnetii type iv secretion system (t4ss) effectors may represent a major class of cd8(+) t-cell antigens, owing to their cytosolic localization. twenty-nine peptides were identified that elicited robust ...	2016	27703037
multicenter evaluation of biofire filmarray meningitis/encephalitis panel for detection of bacteria, viruses, and yeast in cerebrospinal fluid specimens.	rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. comprehensive testing of cerebrospinal fluid (csf) often includes gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. these methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. the filmarray meningitis/encephalitis (me) panel is a multiplexed in vitro di ...	2016	27335149
evaluation of the antioxidative, antibacterial, and anti-inflammatory effects of the aloe fermentation supernatant containing lactobacillus plantarum hm218749.1.	little work is done to develop aloe vera (av) using probiotics. to explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of aloe fermentation supernatant were evaluated in vitro. our results indicated that the aloe fermentation supernatant fermented by lactobacillus plantarum hm218749.1 had very strong scavenging capacities of the dpph (86%), o2 (•-) (85%), (•)oh (76%), and fe(2+) chelation (82%) and reducing powers (242.5 mg/l), and the inh ...	2016	27493450
purification and characterization of a bacteriocin from an oenological strain of leuconostoc mesenteroides subsp. cremoris.	malolactic fermentation (mlf), which improves organoleptic properties and biologic stability of some wines, may cause wine spoilage if uncontrolled. bacteriocins were reported as efficient preservatives to control mlf through their bactericidal effect on malolactic bacteria. leuconostoc mesenteroides subsp. cremoris w3 isolated from wine produces an inhibitory substance that is bactericidal against malolactic bacteria in model wine medium. treatment of the culture supernatant of strain w3 with p ...	2016	25837975
experience with rapid microarray-based diagnostic technology and antimicrobial stewardship for patients with gram-positive bacteremia.	objective to describe the impact of rapid diagnostic microarray technology and antimicrobial stewardship for patients with gram-positive blood cultures. design retrospective pre-intervention/post-intervention study. setting a 1,200-bed academic medical center. patients inpatients with blood cultures positive for staphylococcus aureus, enterococcus faecalis, e. faecium, streptococcus pneumoniae, s. pyogenes, s. agalactiae, s. anginosus, streptococcus spp., and listeria monocytogenes during the 6 ...	2016	27767002
development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging.	consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ...	2016	27455176
antimicrobial and physicochemical characterization of biodegradable, nitric oxide-releasing nanocellulose-chitosan packaging membranes.	biodegradable composite membranes with antimicrobial properties consisting of nanocellulose fibrils (cnfs), chitosan, and s-nitroso-n-acetyl-d-penicillamine (snap) were developed and tested for food packaging applications. as a nitric oxide donor, snap was encapsulated into completely dispersed chitosan in 100 ml of 0.1 n acetic acid and was thoroughly mixed with cnfs to produce a composite membrane. the fabricated membranes had a uniform dispersion of chitosan and snap within the cnfs, which wa ...	2016	27258235
probiotic properties of lactic acid bacteria isolated from human milk.	the objective of this study was to identify and characterize lactic acid bacteria isolated from human milk, with an emphasis on their probiotic properties.	2016	27159339
spatial organization of cell wall-anchored proteins at the surface of gram-positive bacteria.	bacterial surface proteins constitute an amazing repertoire of molecules with important functions such as adherence, invasion, signalling and interaction with the host immune system or environment. in gram-positive bacteria, many surface proteins of the "lpxtg" family are anchored to the peptidoglycan (pg) by an enzyme named sortase. while this anchoring mechanism has been clearly deciphered, less is known about the spatial organization of cell wall-anchored proteins in the bacterial envelope. h ...	2016	27025379
screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species.	in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ...	2016	26907753
tigecycline resistance in clinical isolates of enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(l) and tet(m).	tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including vre and mrsa. the german national reference centre for staphylococci and enterococci has received 73 tigecycline-resistant enterococcus faecium and enterococcus faecalis isolates in recent years. the precise mechanism of how enterococci become resistant to tigecycline remains undetermined. this study documents an analysis of the role of efflux pumps in tigecycline resistance in c ...	2016	26682961
a galectin related protein from oplegnathus fasciatus: genomic, molecular, transcriptional features and biological responses against microbial pathogens.	galectins, a family of β-galactoside-binding lectins, are pattern recognition receptors that recognize pathogen-associated molecular patterns and are subsequently involved in the opsonization, phagocytosis, complement activation, and killing of microbes. here, we report a novel galectin related protein (grp) identified from rock bream (oplegnathus fasciatus), designated ofgal like b. the cdna of ofgal like b is 517 bp with an open reading frame (orf) of 438 bp, encoding 145 amino acids, with a s ...	2016	26615008
chemical content, antibacterial and antioxidant properties of essential oil extract from tunisian origanum majorana l. cultivated under saline condition.	essential oils of marjoram were extracted from plants, growing under non-saline and saline condition (75mm nacl). their antioxidant and antibaterial activity against six bacteria (enterococcus faecalis, escherichia coli, salmonella enteritidis, listeria ivanovii, listeria inocula, and listeria monocytogenes) were assessed. result showed that, (i) independently of salt treatment, marjoram essential oils inhibited the growth of most of the bacteria but in degrees. the least susceptible one was ent ...	2016	28375110
application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria.	bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ...	2016	26042353
knowledge gaps in host-parasite interaction preclude accurate assessment of meat-borne exposure to toxoplasma gondii.	toxoplasma gondii is recognized as a widely prevalent zoonotic parasite worldwide. although several studies clearly identified meat products as an important source of t. gondii infections in humans, quantitative understanding of the risk posed to humans through the food chain is surprisingly scant. while probabilistic risk assessments for pathogens such as campylobacter jejuni, listeria monocytogenes or escherichia coli have been well established, attempts to quantify the probability of human ex ...	2016	28139250
decay rates of zoonotic pathogens and viral surrogates in soils amended with biosolids and manures and comparison of qpcr and culture derived rates.	the purpose of this study was to establish inactivation decay constants of foodborne pathogens and coliphage in clay and sandy soils for future "downstream" analyses such as quantitative microbial risk analysis and to compare cultivation-dependent and -independent (e.g. qpcr) methods.	2016	27585434
efficacy of neutral electrolyzed water, quaternary ammonium and lactic acid-based solutions in controlling microbial contamination of food cutting boards using a manual spraying technique.	bactericidal activity of neutral electrolyzed water (new), quaternary ammonium (quat), and lactic acid-based solutions was investigated using a manual spraying technique against salmonella typhimurium, escherichia coli o157:h7, campylobacter jejuni, listeria monocytogenes and staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting boards. antimicrobial activity was also examined when using cutting boards in preparation of raw chopped beef, chi ...	2016	27027449
validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products.	this study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, campylobacter jejuni, listeria monocytogenes, and salmonella spp., for its actual application in routine food testing. due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using campylobacter enrichment broth for c. jejuni and universal pre-enrichment broth for l. monocytogenes and salmonella ...	2016	26950032
sensitive detection of campylobacter jejuni using nanoparticles enhanced qcm sensor.	a quartz crystal microbalance (qcm) sensor platform was used to develop an immunosensor for the detection of food pathogen campylobacter jejuni. rabbit polyclonal antibodies and commercially available mouse monoclonal antibodies against c. jejuni were investigated to construct direct, sandwich and gold-nanoparticles (aunps) amplified sandwich assays. the performance of the qcm immunosensor developed using sandwich assay by utilising the rabbit polyclonal antibody as the capture antibody and conj ...	2016	26649490
pathogen loading from canada geese faeces in freshwater: potential risks to human health through recreational water exposure.	canada geese (branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. further, increases in both the canada geese populations and their ideal habitat requirements in the united states (us) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. combining these factors, the potential health risk posed by canada geese faeces at freshwater beaches presents an emerging ...	2016	26414207
construction of antimicrobial peptide-drug combination networks from scientific literature based on a semi-automated curation workflow.	considerable research efforts are being invested in the development of novel antimicrobial therapies effective against the growing number of multi-drug resistant pathogens. notably, the combination of different agents is increasingly explored as means to exploit and improve individual agent actions while minimizing microorganism resistance. although there are several databases on antimicrobial agents, scientific literature is the primary source of information on experimental antimicrobial combin ...	2016	28025336
complete genome sequence of lactobacillus plantarum lz206, a potential probiotic strain with antimicrobial activity against food-borne pathogenic microorganisms.	lactobacilli strains have been considered as important candidates for manufacturing "natural food", due to their antimicrobial properties and generally regarded as safe (gras) status. lactobacillus plantarum lz206 is a potential probiotic strain isolated from raw cow milk, with antimicrobial activity against various pathogens, including gram-positive bacteria (staphylococcus aureus and listeria monocytogenes), gram-negtive bacteria (escherichia coli and salmonella enterica), and fungus candida a ...	2016	27671696
development of new 5-(chromene-3-yl)methylene-2,4-thiazolidinediones as antimicrobial agents.	in the context of the increasing phenomenon of microbial resistance to usual drugs, the development of new treatment strategies and new therapeutic protocols is a constant need. thiazolidinedione and chromone represent two important scaffolds in medicinal chemistry due to their large pharmacological applicability.	2016	27004035
bioactive compounds contents, antioxidant and antimicrobial activities during ripening of prunus persica l. varieties from the north west of tunisia.	bioactive molecules from fruits of four varieties of prunus persica at different stages of ripening (green, small orange, red) were studied. for example, contents on polyphenols (20.36mg gae/g fw) and flavonoids (0.764mg re/g fw) were high and varied according variety. the antioxidant activity, using four different tests (dpph radical scavenging activity, reducing power, β carotene bleaching system and tbars assay) showed that the variety chatos exhibited the highest antioxidant activity compari ...	2016	26988472
calcium ions tune the zinc-sequestering properties and antimicrobial activity of human s100a12.	human s100a12 is a host-defense protein expressed and released by neutrophils that contributes to innate immunity. apo s100a12 is a 21-kda antiparallel homodimer that harbors two ca(ii)-binding ef-hand domains per subunit and exhibits two his3asp motifs for chelating transition metal ions at the homodimer interface. in this work, we present results from metal-binding studies and microbiology assays designed to ascertain whether ca(ii) ions modulate the zn(ii)-binding properties of s100a12 and fu ...	2016	26913170
maltaricin cpn, a new class iia bacteriocin produced by carnobacterium maltaromaticum cpn isolated from mould-ripened cheese.	the purpose of this study was to isolate, characterize and determine the structure and the antibacterial activities of a bacteriocin produced by carnobacterium maltaromaticum cpn, a strain isolated from unpasteurized milk camembert cheese.	2016	27489131
formicin - a novel broad-spectrum two-component lantibiotic produced by bacillus paralicheniformis apc 1576.	bacteriocins represent a rather underutilized class of antimicrobials despite often displaying activity against many drug-resistant pathogens. lantibiotics are a post-translationally modified class of bacteriocins, characterized by the presence of lanthionine and methyllanthionine bridges. in this study, a novel two-peptide lantibiotic was isolated and characterized. formicin was isolated from bacillus paralicheniformis apc 1576, an antimicrobial-producing strain originally isolated from the int ...	2016	27450592
an experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins.	human intestinal epithelial cell lines (t84, caco-2, and hct-8) grown on permeable transwell™ filters serve as models of the gastrointestinal barrier. in this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. indicators of cytotoxicity (ldh release, mtt conversion), monolayer barrier integrity ([(3)h]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [teer]), and inflammation (il-8, il- ...	2016	27060235
contribution of selective conditions to microbial competition in four listeria selective enrichment formulations.	microbial competition during selective enrichment negatively affects listeria monocytogenes populations and may hinder the subsequent detection or recovery of this organism. competition assays among 10 selected strains of listeria and citrobacter braakii were performed in buffered listeria enrichment broth, 3-(n-morpholino)propanesulfonic acid-buffered listeria enrichment broth, university of vermont medium-modified listeria enrichment broth, and fraser broth. the individual contributions of eac ...	2016	28221922
the noa gene is functionally linked to the activation of the toll/imd signaling pathways in bactrocera dorsalis (hendel).	the noa gene is an essential gene encoding a very long chain fatty acid elongase. in this study, we cloned the noa gene of bactrocera dorsalis, which encodes a protein sharing 84.50% identity to the noa in drosophila melanogaster. the expression profiles indicated that the transcriptional level of noa was high at the egg stage and in the testis tissue. the results showed that noa expression was up-regulated after listeria monocytogenes, staphylococcus aureus and escherichia coli infection. silen ...	2016	26404497
real-time investigation of antibiotics-induced oxidative stress and superoxide release in bacteria using an electrochemical biosensor.	the involvement of oxidative stress in the mechanism of antibiotics-meditated cell death is unclear and subject to debate. the kinetic profile and a quantitative relationship between the release of reactive oxygen species (ros), bacteria and antibiotic type remain elusive. here we report direct measurements and analytical quantification of the release of superoxide radicals (o2(·-)), a major contributor to ros, in antibiotics-treated bacterial cultures using a cytochrome c electrochemical biosen ...	2016	26655038
biofabrication of zinc oxide nanoparticles using fruit extract of rosa canina and their toxic potential against bacteria: a mechanistic approach.	the use of plant extract in the biosynthesis of nanoparticles (nps) can be an eco-friendly approach and have been suggested as a possible alternative to classic methods namely physical and chemical procedures. in this study, the biosynthesis of zinc oxide (zno) nps by both "conventional heating" (ch) and "microwave irradiation" (mi) methods has been reported. stable and spherical znonps were produced using zinc nitrate and flesh extract of rosa canina fruit (rosehip) which was used as a precurso ...	2016	26652376
bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions.	clove oil (co) anionic nanoemulsions were prepared with varying ratios of co to canola oil (ca), emulsified and stabilized with purity gum ultra (pgu), a newly developed succinylated waxy maize starch. interfacial tension measurements showed that co acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5:5% v/v co:ca) was reached. antimicrobial activity of the negatively charge ...	2016	26616926
the effect of nacl reduction in the microbiological quality of cracked green table olives of the maçanilha algarvia cultivar.	the present work aimed at studying the effect of the partial replacement of nacl with kcl and cacl2 of the fermenting brines on the microbiological quality of natural cracked green maçanilha algarvia table olives. olives were fermented in different salt combinations (brine 1-8% nacl, brine 2-4% nacl 4% kcl, brine 3-4% nacl 4% cacl2, brine 4-4% kcl 4% cacl2, and brine 5-2.7% nacl 2.7% kcl 2.7% cacl2) and the abundance of yeasts and enterobacteria was determined. at the end of fermentation, the ma ...	2016	26613162
characterization of a transferable bcrabc and cadac genes-harboring plasmid in listeria monocytogenes strain isolated from food products of animal origin.	in this study, we characterized a bcrabc cassette and its genetic environment harbored by a plasmid in listeria monocytogenes (l. monocytogenes) 11gzl18, a strain isolated from raw meat in 2011. the bcrabc cassette and its genetic environment were characterized, with a total of 33,727 nt nucleotide sequence obtained. the nucleotide sequences of the bcrabc cassette in strain 11gzl18 exhibited 100% identity to that on plasmid plm80, which is harbored by l. monocytogenes strain h7550 and h7858, and ...	2016	26513251
lactobacillus plantarum lb95 impairs the virulence potential of gram-positive and gram-negative food-borne pathogens in ht-29 and vero cell cultures.	listeria monocytogenes, salmonella enterica and verocytotoxigenic escherichia coli (vtec) are amongst the most important agents responsible for food outbreaks occurring worldwide. in this work, two lactobacillus spp. strains (labs), lactobacillus plantarum (lb95) and lactobacillus paraplantarum (lb13), previously isolated from spontaneously fermenting olive brines, and two reference probiotic strains, lactobacillus casei shirota and lactobacillus rhamnosus gg, were investigated for their ability ...	2016	26506821
development of prototypes of bioactive packaging materials based on immobilized bacteriophages for control of growth of bacterial pathogens in foods.	due to lack of adequate control methods to prevent contamination in fresh produce and growing consumer demand for natural products, the use of bacteriophages has emerged as a promising approach to enhance safety of these foods. this study sought to control listeria monocytogenes in cantaloupes and rte meat and escherichia coli o104:h4 in alfalfa seeds and sprouts under different storage conditions by using specific lytic bacteriophage cocktails applied either free or immobilized. bacteriophage c ...	2016	26490649
pathogen-nematode interaction: nitrogen supply of listeria monocytogenes during growth in caenorhabditis elegans.	listeria monocytogenes is a gram-positive facultatively intracellular human pathogen. due to its saprophytic lifestyle, l. monocytogenes is assumed to infect and proliferate within soil organisms such as caenorhabditis elegans. however, little is known about the nutrient usages and metabolite fluxes in this bacterium-nematode interaction. here, we established a nematode colonization model for l. monocytogenes and a method for the efficient separation of the pathogen from the nematodal gut. follo ...	2016	26478569
development of bioactive fish gelatin/chitosan nanoparticles composite films with antimicrobial properties.	the objective of this work was to develop active bio-based nanocomposite films from fish gelatin (fg) and chitosan nanoparticles (csnps) incorporated with origanum vulgare l. essential oil (oeo). csnps were obtained by ionic gelation of chitosan with sodium tripolyphosphate, which presented a spherical morphology with size range of 40-80nm. remarkable differences in the surface morphology were observed between the control and bioactive nanocomposite films as revealed by sem and afm images. ftir ...	2016	26471681
identification of lactic acid bacteria in fruit pulp processing byproducts and potential probiotic properties of selected lactobacillus strains.	this study aimed to identify lactic acid bacteria (lab) in byproducts of fruit (malpighia glabra l., mangifera indica l., annona muricata l., and fragaria vesca l.) pulp processing. fifty strains of lab were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequence (16s rrna) analysis. species belonging to lactobacillus genus were the predominant lab in all fruit pulp processing byproducts. the average congruency betwe ...	2016	27625647
comprehensive evaluation and implementation of improvement actions in butcher shops.	foodborne pathogens can cause acute and chronic diseases and produce a wide range of symptoms. since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. a comprehensive evaluation was made and risk was quantified on a 1-100 scale as high-risk (1-40), moderate-risk (41-70) or ...	2016	27618439
presence of escherichia coli o157, salmonella spp., and listeria monocytogenes in raw ovine milk destined for cheese production and evaluation of the equivalence between the analytical methods applied.	italy is one of the main producers and exporters of cheese made from unpasteurized sheep milk. since raw milk and its derived products are known sources of human infections, cheese produced from raw sheep milk could pose a microbiological threat to public health. hence, the objectives of the study were: to characterize the potential risk of the presence of pathogens escherichia coli o157, listeria monocytogenes, and salmonella in raw ovine milk destined for cheese production obtained from all th ...	2016	27607835
development of a sandwich elisa for ehec o157:h7 intimin γ1.	enterohemorrhagic escherichia coli (ehec) o157:h7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for ehec's intimate adhesion to epithelial cells. here, we generated a polyclonal antibody (pab) and a monoclonal antibody (mab) against intimin γ1 to develop a double antibody sandwich elisa (das-elisa) with increased sensitivity and specificity for measuring ehec o1 ...	2016	27603508
characterization of egg white antibacterial properties during the first half of incubation: a comparative study between embryonated and unfertilized eggs.	egg white is an important contributor to the protection of eggs against bacterial contaminations during the first half of incubation (day zero to 12), prior to the egg white transfer into the amniotic fluid to be orally absorbed by the embryo. this protective system relies on an arsenal of antimicrobial proteins and on intrinsic physicochemical properties that are generally unfavorable for bacterial multiplication and dissemination. some changes in these parameters can be observed in egg white d ...	2016	27601682
do bacterial cell numbers follow a theoretical poisson distribution? comparison of experimentally obtained numbers of single cells with random number generation via computer simulation.	we investigated a bacterial sample preparation procedure for single-cell studies. in the present study, we examined whether single bacterial cells obtained via 10-fold dilution followed a theoretical poisson distribution. four serotypes of salmonella enterica, three serotypes of enterohaemorrhagic escherichia coli and one serotype of listeria monocytogenes were used as sample bacteria. an inoculum of each serotype was prepared via a 10-fold dilution series to obtain bacterial cell counts with me ...	2016	27554145
modeling optimal process conditions for uv-heat inactivation of foodborne pathogens in liquid foods.	the combination of ultraviolet radiation and heat (uv-h treatment) has been demonstrated as a promising strategy to overcome the limited uv germicidal effect in fruit juices. nonetheless, there are so far no data regarding the efficacy of the combined process for the inactivation of bacterial foodborne pathogens in other liquid foods with different ph and composition. in this investigation, the optimum uv-h processing conditions for the inactivation of escherichia coli, salmonella typhimurium, l ...	2016	27554141