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listeria monocytogenes bacteremia in a pancreas transplant recipient.we report a case of listeria monocytogenes bacteremia in a patient 12 years after his pancreas transplant, during which time he received a steroid-free immunosuppressive regimen. to our knowledge, there are no reported cases describing l monocytogenes bacteremia after pancreas transplant. in addition, although typically identified as a complication shortly after transplant or after treatment for organ rejection, this case demonstrates that it is still possible for a patient to develop a l monocy ...201526645926
interleukin-22-induced antimicrobial phospholipase a2 group iia mediates protective innate immunity of nonhematopoietic cells against listeria monocytogenes.listeria monocytogenes is a bacterial pathogen which establishes intracellular parasitism in various cells, including macrophages and nonhematopoietic cells, such as hepatocytes. it has been reported that several proinflammatory cytokines have pivotal roles in innate protection against l. monocytogenes infection. we found that a proinflammatory cytokine, interleukin 22 (il-22), was expressed by cd3(+) cd4(+) t cells at an early stage of l. monocytogenes infection in mice. to assess the influence ...201526644377
choice of reference-guided sequence assembler and snp caller for analysis of listeria monocytogenes short-read sequence data greatly influences rates of error.the influences that different programs and conditions have on error rates of single-nucleotide polymorphism (snp) analyses are poorly understood. using illumina short-read sequence data generated from listeria monocytogenes strain hpb5622, we assessed the performance of four snp callers (bcftools, freebayes, unifiedgenotyper, varscan) under a variety of conditions, including: (1) a range of sequencing coverages; (2) use of four popular reference-guided assemblers (burrows-wheeler aligner, novoal ...201526643440
biosynthesis of paenibacillin, a lantibiotic with n-terminal acetylation, by paenibacillus polymyxa.paenibacillin, a recently-discovered lantibiotic from paenibacillus polymyxa osy-df, showed potency against listeria monocytogenes, methicillin-resistant staphylococcus aureus and other gram-positive bacteria. the chemical structure of paenibacillin has been determined previously. this study was initiated to investigate the biosynthesis of paenibacillin, and to reveal unique features in its biosynthetic pathway. paenibacillin structural gene (paea) was identified by polymerase chain reaction (pc ...201526640048
listeriosis phytotherapy: a review study on the effectiveness of iranian medicinal plants in treatment of listeriosis.listeria monocytogenes can be found in many processed foods, raw milk, dairy products, meat and meat products such as sausages, beef and fish products, seafoods, eggs, fruits, and vegetables such as radish and cabbage. this article is a review study on the iranian medicinal plants applied for treatment of listeriosis. information of this review article was obtained by searching various key words such as listeria monocytogenes, medicinal plants, plant extracts and essential oils among scientific ...201526682768
the novel multiple inner primers-loop-mediated isothermal amplification (mip-lamp) for rapid detection and differentiation of listeria monocytogenes.here, a novel model of loop-mediated isothermal amplification (lamp), termed multiple inner primers-lamp (mip-lamp), was devised and successfully applied to detect listeria monocytogenes. a set of 10 specific mip-lamp primers, which recognized 14 different regions of target gene, was designed to target a sequence in the hlya gene. the mip-lamp assay efficiently amplified the target element within 35 min at 63 °c and was evaluated for sensitivity and specificity. the templates were specially ampl ...201526633345
[infectious diseases in the genomic era].next generation sequencing (ngs) technologies have arrived, changing research and infectious disease research into a new era, the "genomic era". currently, the developed world is introducing ngs in a number of applications, including clinical diagnostics, epidemiology, and microbiology. in developing countries ngs is being progressively introduced. technologies currently available allow to sequence the whole genome of bacterial and viral strains for an approximate cost of $100 usd, which is high ...201526633116
[sanitary significance of soil sea coasts].there was investigated the dynamics of growth of listeria monocytogenes and yersinia pseudotuberculosis in soils of sea coast (mid-flight and maritime soils). these bacteria were shown to reproduced well in all researched soils, preferring nevertheless maritime soils. the content of the humus was determined to be the one of the limiting factors restricting the multiplication of pathogenic bacteria in studied soils. abiotic characteristic of soils of sea coast were established to render the direc ...201526625609
prevalence of listeria monocytogenes in raw bovine milk and milk products from central highlands of ethiopia.listeria monocytogenes is of major significance in human and veterinary medicine. most human listeria infections are foodborne and the association of contaminated milk and dairy produce consumption with human listeriosis is noteworthy. in ethiopia, there is limited data regarding the prevalence of l. monocytogenes in raw bovine milk and dairy products. the aim of this study was, therefore, to determine the prevalence of l. monocytogenes in raw bovine milk and dairy produce.201526623629
examination of listeria monocytogenes in seafood processing facilities and smoked salmon in the republic of ireland.listeria monocytogenes is a foodborne pathogen that causes listeriosis, a relatively rare but life-threatening disease primarily affecting immunocompromised individuals. the aim of this study was to determine the prevalence of l. monocytogenes in the seafood processing industry in the republic of ireland. the occurrence of l. monocytogenes was determined by regular sampling of both food samples and processing environment swabs at eight seafood processing facilities over two calendar years. all s ...201526613913
tolerance of listeria monocytogenes to quaternary ammonium sanitizers is mediated by a novel efflux pump encoded by emre.a novel genomic island (lgi1) was discovered in listeria monocytogenes isolates responsible for the deadliest listeriosis outbreak in canada, in 2008. to investigate the functional role of lgi1, the outbreak strain 08-5578 was exposed to food chain-relevant stresses, and the expression of 16 lgi1 genes was measured. lgi1 genes with putative efflux (l. monocytogenes emre [emrelm]), regulatory (lmo1851), and adhesion (sel1) functions were deleted, and the mutants were exposed to acid (hcl), cold ( ...201526590290
determination of evolutionary relationships of outbreak-associated listeria monocytogenes strains of serotypes 1/2a and 1/2b by whole-genome sequencing.we used whole-genome sequencing to determine evolutionary relationships among 20 outbreak-associated clinical isolates of listeria monocytogenes serotypes 1/2a and 1/2b. isolates from 6 of 11 outbreaks fell outside the clonal groups or "epidemic clones" that have been previously associated with outbreaks, suggesting that epidemic potential may be widespread in l. monocytogenes and is not limited to the recognized epidemic clones. pairwise comparisons between epidemiologically related isolates wi ...201526590286
processing-dependent and clonal contamination patterns of listeria monocytogenes in the cured ham food chain revealed by genetic analysis.the quantitative and qualitative patterns of environmental contamination by listeria monocytogenes were investigated in the production chain of dry-cured parma ham. standard arrays of surfaces were sampled in processing facilities during a single visit per plant in the three compartments of the food chain, i.e., ham production (19 plants) and postproduction, which was divided into deboning (43 plants) and slicing (25 plants) steps. the numbers of sampled surfaces were 384 in ham production, with ...201526590278
listeria monocytogenes: strain heterogeneity, methods, and challenges of subtyping.listeria monocytogenes is a food-borne bacterial pathogen that is associated with 20% to 30% case fatality rate. l. monocytogenes is a genetically heterogeneous species, with a small fraction of strains (serotypes 1/2a, 1/2b, 4b) implicated in human listeriosis. monitoring and source tracking of l. monocytogenes involve the use of subtyping methods, with the performance of genetic-based methods found to be superior to phenotypic-based ones. various methods have been used to subtype l. monocytoge ...201526588067
smad4 represses the generation of memory-precursor effector t cells but is required for the differentiation of central memory t cells.the transcriptional regulation underlying the differentiation of cd8(+) effector and memory t cells remains elusive. here, we show that 18-month-old mice lacking the transcription factor smad4 (homolog 4 of mothers against decapentaplegic, drosophila), a key intracellular signaling effector for the tgf-β superfamily, in t cells exhibited lower percentages of cd44(hi)cd8(+) t cells. to explore the role of smad4 in the activation/memory of cd8(+) t cells, 6- to 8-week-old mice with or without smad ...201526583325
economic cost of a listeria monocytogenes outbreak in canada, 2008.estimates of the economic costs associated with foodborne disease are important to inform public health decision-making. in 2008, 57 cases of listeriosis and 24 deaths in canada were linked to contaminated delicatessen meat from one meat processing plant. costs associated with the cases (including medical costs, nonmedical costs, and productivity losses) and those incurred by the implicated plant and federal agencies responding to the outbreak were estimated to be nearly $242 million canadian do ...201526583272
health trajectories reveal the dynamic contributions of host genetic resistance and tolerance to infection outcome.resistance and tolerance are two alternative strategies hosts can adopt to survive infections. both strategies may be genetically controlled. to date, the relative contribution of resistance and tolerance to infection outcome is poorly understood. here, we use a bioluminescent listeria monocytogenes (lm) infection challenge model to study the genetic determination and dynamic contributions of host resistance and tolerance to listeriosis in four genetically diverse mouse strains. using convention ...201526582028
a detailed view of the intracellular transcriptome of listeria monocytogenes in murine macrophages using rna-seq.listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which is mainly a threat for pregnant, elderly, or immunocompromised individuals. due to its ability to invade and colonize diverse eukaryotic cell types including cells from invertebrates, l. monocytogenes has become a well-established model organism for intracellular growth. almost 10 years ago, we and others presented the first whole-genome microarray-based intracellular transcriptome o ...201526579105
growth and inactivation of salmonella enterica and listeria monocytogenes in broth and validation in ground pork meat during simulated home storage abusive temperature and home pan-frying.ground pork meat with natural microbiota and inoculated with low initial densities (1-10 or 10-100 cfu/g) of salmonella enterica or listeria monocytogenes was stored under abusive temperature at 10°c and thermally treated by a simulated home pan-frying procedure. the growth and inactivation characteristics were also evaluated in broth. in ground pork meat, the population of s. enterica increased by less than one log after 12-days of storage at 10°c, whereas l. monocytogenes increased by 2.3 to 2 ...201526579079
in-vitro antioxidant and antibacterial properties of fermentatively and enzymatically prepared chicken liver protein hydrolysates.protein hydrolysates were prepared from chicken liver using fermentation and enzymatic hydrolysis. the lactic acid bacteria pediococcus acidilactici ncim5368 was employed in the fermentation process and a commercial protease (alcalase® 2.5) was used in enzymatic hydrolysis. chicken liver hydrolysates prepared by fermentation (fclh) and enzymatic hydrolysis (eclh) revealed appreciable amounts of protein [55.85 and 61.34 %; on dry weight basis, respectively]. fermentation and enzymatic hydrolysis ...201526604378
rapid identification and classification of listeria spp. and serotype assignment of listeria monocytogenes using fourier transform-infrared spectroscopy and artificial neural network analysis.the use of fourier transform-infrared spectroscopy (ft-ir) in conjunction with artificial neural network software neurodeveloper™ was examined for the rapid identification and classification of listeria species and serotyping of listeria monocytogenes. a spectral library was created for 245 strains of listeria spp. to give a biochemical fingerprint from which identification of unknown samples were made. this technology was able to accurately distinguish the listeria species with 99.03% accuracy. ...201526600423
risk factors for sporadic non-pregnancy associated listeriosis in germany-immunocompromised patients and frequently consumed ready-to-eat products.non-pregnancy associated (n-pa) listeriosis, caused by listeria monocytogenes, is a rare but severe disease, and is predominantly food-borne. most cases appear sporadic and their infection vehicle remains unknown. incidence has increased since 2008 in germany. we aimed to identify underlying conditions and foods associated with sporadic n-pa listeriosis in germany. we performed a nationwide case-control study from march 2012-december 2013. cases were sporadic n-pa listeriosis patients notified t ...201526599484
autophagy regulates t lymphocyte proliferation through selective degradation of the cell-cycle inhibitor cdkn1b/p27kip1.the highly conserved cellular degradation pathway, macroautophagy, regulates the homeostasis of organelles and promotes the survival of t lymphocytes. previous results indicate that atg3-, atg5-, or pik3c3/vps34-deficient t cells cannot proliferate efficiently. here we demonstrate that the proliferation of atg7-deficient t cells is defective. by using an adoptive transfer and listeria monocytogenes (lm) mouse infection model, we found that the primary immune response against lm is intrinsically ...201526569626
a delayed cerebral vasospasm with infarction is secondary to listeria monocytogenes meningitis: mri and mra are diagnostically useful.among patients with bacterial meningitis, a cerebral vasospasm typically occurs during the acute phase. we experienced a case of delayed cerebral vasospasm with infarction that was secondary to listeria monocytogenes meningitis. an 82-year-old woman with listeria monocytogenes meningitis, whose symptoms had been improving after the initiation of antibacterial therapy, fell into a coma on day 15 and developed generalized seizure. magnetic resonance imaging (mri) and mr angiography (mra) indicated ...201526568013
pectin and xyloglucan influence the attachment of salmonella enterica and listeria monocytogenes to bacterial cellulose-derived plant cell wall models.minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. these pathogens must attach to the produce in order to be transmitted. cut surfaces of produce that expose cell walls are particularly vulnerable. little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. using bacterial cellulose-derived plant cell wall models ...201526567310
an interactomic approach for identification of putative drug targets in listeria monocytogenes.a wide variety of human population is infected with listeria monocytogenes, which causes listeriosis, a deadly disease with mortality rate of about 30%. the major hindrance to cure listeriosis is the unavailability of specific or selectable drug targets. at present, antibiotics used to cure the disease are not specific and insufficient to manage the disease efficiently. therefore, in order to search specific drugs, here, we used interactome analysis to search specific drug targets which may prov ...201526561317
antimicrobial and antioxidant activities of enterococcus species isolated from meat and dairy products.abstractlactic acid bacteria (lab) have an important role in a great variety of fermented foods. in addition to their contribution to sensory characteristics, they enhance food preservation and can be used as probiotics. in this study, the antimicrobial and antioxidant activities of culture supernatants and cell free extracts of 16 lab isolated from meat and dairy products were investigated. the bacterial were identified by 16s rrna sequencing. genbank blast analysis revealed that all the isolat ...201526560661
prevalence of foodborne pathogens in freshwater fish in latvia.the aim of this study was to detect the prevalence of salmonella spp., listeria monocytogenes, and yersinia enterocolitica in freshwater fish in latvia. in total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from april 2014 to december 2014 in latvia. samples were tested according to international organization for standardization methods. no salmonella spp. were found in fresh fish from lakes or in commercially avail ...201526555535
predominance and distribution of a persistent listeria monocytogenes clone in a commercial fresh mushroom processing environment.a longitudinal study was conducted to determine the prevalence of listeria spp. in a commercial fresh mushroom slicing and packaging environment. samples were collected at three different sampling periods within a 13-month time interval. of the 255 environmental samples collected, 18.8% tested positive for l. monocytogenes, 4.3% for l. innocua, and 2.0% for l. grayi. l. monocytogenes was most often found on wet floors within the washing and slicing and packaging areas. each of the 171 l. monocyt ...201526555522
disease burden of invasive listeriosis and molecular characterization of clinical isolates in taiwan, 2000-2013.the information about disease burden and epidemiology of invasive listeriosis in asia is scarce. from 2000 to 2013, a total of 338 patients with invasive listeriosis (bacteremia, meningitis, and peritonitis) were treated at four medical centers in taiwan. the incidence (per 10,000 admissions) of invasive listeriosis increased significantly during the 14-year period among the four centers (0.15 in 2000 and >1.25 during 2010-2012) and at each of the four medical centers. among these patients, 45.9 ...201526555445
listeria monocytogenes associated kerato-conjunctivitis in four horses in norway.listeria monocytogenes has been reported to cause various infectious diseases in both humans and animals. more rarely, ocular infections have been reported. to our knowledge, only two cases of listeria keratitis have been described in horses. we report kerato-conjunctivitis in four norwegian horses associated with l. monocytogenes. clinically, all cases were presented with recurrent unilateral kerato-conjunctivitis. l. monocytogenes bacteria were isolated from swab samples from all cases, and cy ...201526552393
induced systemic listeriosis in alectoris chukar chicks: clinical, histopathological and microbiological findings.1. systemic listeriosis was induced in 14-d-old chukar partridge chicks, alectoris chukar, by intravenous injection of a suspension containing 10(6) cfu/ml of viable listeria monocytogenes organisms to study the course of infection. 2. septicaemic and encephalitic forms of listeriosis were observed in all birds. infection resulted in a fever response 8-h post-inoculation. disease rapidly developed over a 24-h period with decreased activity, lethargy, ruffled feathers, huddling, listlessness, ina ...201526551997
mir-150 regulates differentiation and cytolytic effector function in cd8+ t cells.micrornas regulate most mammalian genes, and they control numerous aspects of immune system development and function. their precise roles in the cd8+ t cell response, however, remain unclear. in this report, we show that in the absence of the microrna mir-150, cd8+ t cells fail to undergo robust expansion and differentiation into short-lived terminal effector cells in response to primary infection with listeria monocytogenes or vaccinia virus. notably, even after transitioning into the memory po ...201526549197
the opposite effects of dna and protein components of listeria monocytogenes and toxoplasma gondii on immunologic characteristics of dendritic cells.the innate immune system utilizes pattern recognition receptors (prrs) to recognize microbes. pathogens contain various molecules with diverse effects on immune response. in this study, we evaluated the effect of dna and protein components derived from two intracellular microorganisms including listeria monocytogenes (l. monocytogenes) and toxoplasma gondii (t. gondii) on dendritic cells (dcs) activation and ensuing adaptive immune responses. dna and protein components of l. monocytogenes and t. ...201526546901
foodborne pathogens prevention and sensory attributes enhancement in processed cheese via flavoring with plant extracts.cheese contaminations with foodborne bacterial pathogens, and their health outbreaks, are serious worldwide problems that could happen from diverse sources during cheese production or storage. plants, and their derivatives, were always regarded as the potential natural and safe antimicrobial alternatives for food preservation and improvement. the extracts from many plants, which are commonly used as spices and flavoring agents, were evaluated as antibacterial agents against serious foodborne pat ...201526540146
survival of unstressed and acid-, cold-, and starvation-stress-adapted listeria monocytogenes in ham extract with hops beta acids and consumer acceptability of hba on ready-to-eat ham.the efficacy of hops beta acids (hba) against unstressed and stress-adapted listeria monocytogenes in ham extract and the consumers' acceptability of hba on ready-to-eat (rte) hams were investigated. unstressed or acid-, cold-, or starvation-stress-adapted l. monocytogenes was inoculated (1.3-1.5 log cfu/ml) into 10% ham extract, without (control) or with hba (4.44 or 10.0 µg/ml). survival/growth of the pathogen during storage (7.2 °c, 26 days) was monitored periodically. sensory evaluation (30 ...201526539527
impact of moderate heat, carvacrol, and thymol treatments on the viability, injury, and stress response of listeria monocytogenes.the microbial safety and stability of minimally processed foods are based on the application of combined preservative factors. since microorganisms are able to develop adaptive networks to survive under conditions of stress, food safety may be affected, and therefore understanding of stress adaptive mechanisms plays a key role in designing safe food processing conditions. in the present study, the viability and the sublethal injury of listeria monocytogenes exposed to moderate heat (55 °c) and/o ...201526539510
twenty years of listeria in brazil: occurrence of listeria species and listeria monocytogenes serovars in food samples in brazil between 1990 and 2012.listeria spp. isolated from different food products and collected from 12 brazilian states were sent to the laboratory of bacterial zoonoses (oswaldo cruz institute, brazil) for identification. the aims of this study were to characterize these isolates, from 1990 to 2012, by using biochemical, morphological, and serotyping tests, and to analyze the distribution of l. monocytogenes serotypes on different food products and geographical locations. serotyping was performed using polyclonal somatic a ...201526539507
molecular epidemiology of invasive listeriosis due to listeria monocytogenes in a spanish hospital over a nine-year study period, 2006-2014.we investigated the pathogenicity, invasiveness, and genetic relatedness of 17 clinical listeria monocytogenes stains isolated over a period of nine years (2006-2014). all isolates were phenotypically characterised and growth patterns were determined. the antimicrobial susceptibility of l. monocytogenes isolates was determined in e-tests. invasion assays were performed with epithelial hela cells. finally, l. monocytogenes isolates were subtyped by pfge and mlst. all isolates had similar phenotyp ...201526539467
highly invasive listeria monocytogenes strains have growth and invasion advantages in strain competition.multiple listeria monocytogenes strains can be present in the same food sample; moreover, infection with more than one l. monocytogenes strain can also occur. in this study we investigated the impact of strain competition on the growth and in vitro virulence potential of l. monocytogenes. we identified two strong competitor strains, whose growth was not (or only slightly) influenced by the presence of other strains and two weak competitor strains, which were outcompeted by other strains. cell co ...201526529510
comparative evaluation of veriflow® listeria monocytogenes to usda and aoac culture based methods for the detection of listeria monocytogenes in food.veriflow® listeria monocytogenes (lm) is a molecular based assay for the presumptive detection of listeria monocytogenes from environmental surfaces, dairy, and ready-to-eat (rte) food matrixes (hot dogs and deli meat). the assay utilizes a pcr detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post pcr amplification and requires only 24 h of enrichment for maximum sensitivity. the veriflow lm system eliminates the need for sample purification, gel electrophor ...201526525251
method modification of the thermo scientific suretect listeria monocytogenes assay for raw meat, dairy, produce, and seafood.the thermo scientific™ suretect™ listeria monocytogenes assay is a real-time pcr assay for the detection of listeria monocytogenes in food and environmental samples, which was certified during 2013 by the aoac research institute (ri) as performance tested method(sm) (ptm) 061302 for a representative range of key food matrixes and production surfaces. this report details the method modification study, which was conducted during 2014, using the aoac-ri ptm program to extend the validated matrix cl ...201526525250
biofilm formation of the l. monocytogenes strain 15g01 is influenced by changes in environmental conditions.listeria monocytogenes 15g01, a strain belonging to the persistent pulsotype 5132, was isolated from a seafood processing plant in new zealand. simple monoculture assays using crystal violet staining showed good biofilm formation for this strain and it was therefore chosen to be further investigated in regard to its biofilm forming ability. to evaluate its behaviour in different conditions commonly encountered in food processing environments, biofilm assays and growth studies were performed usin ...201526524221
a tail of two phages: genomic and functional analysis of listeria monocytogenes phages vb_lmos_188 and vb_lmos_293 reveal the receptor-binding proteins involved in host specificity.the physical characteristics of bacteriophages establish them as viable candidates for downstream development of pathogen detection assays and biocontrol measures. to utilize phages for such purposes, a detailed knowledge of their host interaction mechanisms is a prerequisite. there is currently a wealth of knowledge available concerning gram-negative phage-host interaction, but little by comparison for gram-positive phages and listeria phages in particular. in this research, the lytic spectrum ...201526500641
cd8 t cells enter the splenic t cell zones independently of ccr7, but the subsequent expansion and trafficking patterns of effector t cells after infection are dysregulated in the absence of ccr7 migratory cues.ccr7 is an important chemokine receptor that regulates t cell trafficking and compartmentalization within secondary lymphoid organs. however, the t cell-intrinsic role of ccr7 during infection in the spleen is not well understood. this study was designed to understand how ccr7-dependent localization and migration of cd8(+) t cells in different compartments of the spleen affected the primary and recall responses after infection. to this end, we used adoptive transfer of naive ag-specific cd8 t ce ...201526500349
the connection between persistent, disinfectant-resistant listeria monocytogenes strains from two geographically separate iberian pork processing plants: evidence from comparative genome analysis.the aim of this study was to investigate the basis of the putative persistence of listeria monocytogenes in a new industrial facility dedicated to the processing of ready-to-eat (rte) iberian pork products. quaternary ammonium compounds, which included benzalkonium chloride (bac), were repeatedly used as surface disinfectants in the processing plant. clean and disinfected surfaces were sampled to evaluate if resistance to disinfectants was associated with persistence. of the 14 isolates obtained ...201526497458
synergistic effect of copper and low temperature over listeria monocytogenes.the capacity to grow at low temperatures has allowed listeria monocytogenes to become one of the primary food pathogens to date, representing a major public health problem worldwide. several works have described the homeostatic response of l. monocytogenes under different copper (cu) treatments growing at mild temperature (30 °c). the aims of this report were to evaluate if changes in the external concentration of cu affected viability and cu homeostasis of l. monocytogenes growing at low temper ...201526515293
clonal clustering using 10-gene multilocus sequence typing reveals an association between genotype and listeria monocytogenes maximum growth rate in defined medium.we used a 10-gene (10g) multilocus sequence typing scheme to investigate the diversity and phylogenetic distribution of 124 listeria monocytogenes strains across major lineages, major serotypes, and seven epidemic clones that have been previously associated with outbreaks. the 124 isolates proved to be diverse, with a total of 81 sequence types (10g-sts) belonging to 13 clonal complexes (ccs), where all sts of the same cc differ from one another in up to 3 of the 10 alleles (named as 10g-triple- ...201526495863
antagonizing peroxisome proliferator-activated receptor α activity selectively enhances th1 immunity in male mice.females exhibit more robust th1 responses than males. our previous work suggested that this sex disparity is a consequence of higher activity of the androgen-induced gene peroxisome proliferator-activated receptor α (pparα) in male cd4(+) t cells. the objective of this study was to elucidate the cellular and molecular mechanism of how pparα inhibits th1 responses in male mice. in this study, we found that pparα functions within cd4(+) and cd8(+) t lymphocytes and nkt cells to negatively regulate ...201526491197
the listeria monocytogenes core-genome sequence typer (lmcgst): a bioinformatic pipeline for molecular characterization with next-generation sequence data.next-generation sequencing provides a powerful means of molecular characterization. however, methods such as single-nucleotide polymorphism detection or whole-chromosome sequence analysis are computationally expensive, prone to errors, and are still less accessible than traditional typing methods. here, we present the listeria monocytogenes core-genome sequence typing method for molecular characterization. this method uses a high-confidence core (hcc) genome, calculated to ensure accurate identi ...201526490433
novel active surface prepared by embedded functionalized clays in an acrylate coating.the research on a self-decontaminating surface has received significant attention because of the growth of pathogenic microorganisms on surfaces. in this study, a novel and simple technique for producing an active surface with antimicrobial functionality is demonstrated. a tethering platform was developed by grafting the biocide ampicillin (amp) to a nanoclay and dispersing the nanoclay in a uv-curable acrylate coating applied on polypropylene films as the substrate. a coupling agent, [3-(glycid ...201526488557
prevalence, enumeration, and pheno- and genotypic characteristics of listeria monocytogenes isolated from raw foods in south china.listeria monocytogenes is an important foodborne pathogen that can cause serious illness in immunocompromised individuals, pregnant women, the elderly, and newborns. the aim of this study was to: (i) evaluate the prevalence and contamination level [most probable number (mpn)] of l. monocytogenes in 567 retail raw foods (fishery products, n = 154; raw/fresh meat, n = 123; frozen foods, n = 110; edible fungi, n = 108; vegetables, n = 72) collected from south china and (ii) to gain further knowledg ...201526483765
the first mollusk spätzle homolog gene in the clam, paphia undulate.spätzle, is the only identified endogenous toll receptor ligand, plays a critical role in initiatinge innate immune responses and controlling dorsal-ventral axis formation in drosophila. here we identified the first spätzle gene homolog, pu-spz, in the marine mollusk paphia undulate. the full-length of pu-spz cdna is 1248 bp, including an open reading frame (orf) of 702 bp, a 5'-untranslated region (utr) of 26 bp and a 3'-utr of 203 bp. the orf encodes a 233-amino-acid protein with conserved dom ...201526477575
occurrence of salmonella, listeria monocytogenes, and enterotoxigenic staphylococcus in goat milk from small and medium-sized farms located in minas gerais state, brazil.consumption of goat milk has been increasing due to its nutritional characteristics and health benefits. therefore, assessment of the presence of foodborne pathogens in this product is critical to ensure its safety to consumers. the present study aimed to identify common foodborne pathogens in raw goat milk. fifty-three samples of raw goat milk from 11 farms were collected and cultured for the presence of salmonella spp. and listeria monocytogenes, as well as for enumeration and isolation of coa ...201526476939
polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of listeria monocytogenes.infections caused by foodborne pathogens such as listeria monocytogenes pose a threat to public health while timely detection is challenging due to pathogen low numbers. the development of robust and efficient sample preparation techniques is crucial to improve detection sensitivity and workflow. immunomagnetic separation using magnetic nanoparticles (mnps) is attractive, as it can efficiently capture target cells. for food safety applications, a platform is needed to rapidly process large sampl ...201526346021
production of antilisterial bacteriocins from lactic acid bacteria in dairy-based media: a comparative study.one hundred and eight strains of lactic acid bacteria (lab) were screened for bacteriocin production by the modified deferred antagonism and agar well diffusion methods. when the modified deferred antagonism method was employed, 82 lab strains showed inhibitory action against listeria monocytogenes v7 ½a, whereas 26 lab strains expressed no inhibition. only 12 lab strains exhibited inhibitory activity when the agar well diffusion method was used, 11 of which had been previously recognized as bac ...201526341641
estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions: effect of ultrasound on temperature and survival profiles.estimation of listeria monocytogenes survival during thermoultrasonic treatments in non-isothermal conditions was determined considering an increment from 45 to 70 °c, assessing the adequacy of predictions through experimental data obtained in laboratory media model systems. in order to characterize the sonication effect on the survival pattern, observed behavior was compared to that obtained when only thermal treatment was applied. a noticeable impact on l. monocytogenes survival in non-isother ...201526338125
bacteriophage cocktail significantly reduces or eliminates listeria monocytogenes contamination on lettuce, apples, cheese, smoked salmon and frozen foods.listshield™, a commercially available bacteriophage cocktail that specifically targets listeria monocytogenes, was evaluated as a bio-control agent for l. monocytogenes in various ready-to-eat foods. listshield™ treatment of experimentally contaminated lettuce, cheese, smoked salmon, and frozen entrèes significantly reduced (p < 0.05) l. monocytogenes contamination by 91% (1.1 log), 82% (0.7 log), 90% (1.0 log), and 99% (2.2 log), respectively. listshield™ application, alone or combined with an ...201526338115
erratum: increased spread and replication efficiency of listeria monocytogenes in organotypic brain-slices is related to multilocus variable number of tandem repeat analysis (mlva) complex. 201526335249
antimicrobial poly(lactic acid)-based nanofibres developed by solution blow spinning.the present study reports on the development of hybrid poly(lactic acid) (pla) fibres loaded with highly crystalline bacterial cellulose nanowhiskers (bcnw) by the novel solution blow spinning method. furthermore, fibres with antimicrobial properties were generated by incorporating carvacrol and thc as antimicrobial agents and the biocide effect against listeria monocytogenes was studied. initially, pla blow spun fibres containing bcnw were optimized in terms of morphology and thermal properties ...201526328414
behaviour of listeria monocytogenes in artisanal raw milk pecorino umbro cheese: a microbiological challenge test.in the present study, a microbiological challenge test in artificially contaminated raw milk pecorino umbro cheese during cheese-making was carried out. raw ewe milk was contaminated by a suspension of particular listeria monocytogenes strains. the number of l. monocytogenes and l. monocytogenes dynamic growth were evaluated during cheese-making and storage. a significant decrease of the viable count of l. monocytogenes was observed during ripening and l. monocytogenes viable count was below the ...201527800412
diversity of listeria monocytogenes within a u.s. dairy herd, 2004-2010.listeria monocytogenes, the causative agent of listeriosis, is frequently isolated from the environment. dairy cows and dairy farm environments are reservoirs of this pathogen, where fecal shedding contributes to its environmental dispersal and contamination of milk, dairy products, and meat. the molecular diversity of 40 l. monocytogenes isolates representing 3 serogroups (1/2a, 1/2b, and 4b) collected between 2004 and 2010 from the feces of dairy cattle on a single dairy farm was assessed usin ...201526325149
chloroxyanion residue quantification in cantaloupes treated with chlorine dioxide gas.previous studies show that treatment of cantaloupes with chlorine dioxide (clo2) gas at 5 mg/liter for 10 min results in a significant reduction (p < 0.05) in initial microflora, an increase in shelf life without any alteration in color, and a 4.6- and 4.3-log reduction of escherichia coli o157:h7 and listeria monocytogenes, respectively. however, this treatment could result in the presence of chloroxyanion residues, such as chloride (cl(-)), chlorite (clo2(-)), chlorate (clo3(-)), and perchlora ...201526319725
modeling the effect of storage temperatures on the growth of listeria monocytogenes on ready-to-eat ham and sausage.the aim of this study was to model the growth kinetics of listeria monocytogenes on ready-to-eat ham and sausage at different temperatures (4 to 35°c). the observed data fitted well with four primary models (baranyi, modified gompertz, logistic, and huang) with high coefficients of determination (r(2) > 0.98) at all measured temperatures. after the mean square error (0.009 to 0.051), bias factors (0.99 to1.06), and accuracy factors (1.01 to 1.09) were obtained in all models, the square root and ...201526319721
putative cross-contamination routes of listeria monocytogenes in a meat processing facility in romania.putative routes of listeria monocytogenes contamination, based on the workflow of the employees, were studied in a meat processing facility by investigating 226 samples collected from food contact surfaces, non-food contact surfaces, raw materials, and ready-to-eat meat products on four occasions over a 1-year period. in total, 19.7% of non-food contact surfaces, 22.9% of food contact surfaces, 45% of raw materials, and 20% of ready-to-eat meat products were positive for l. monocytogenes (analyz ...201526319720
effect of alginate coatings with cinnamon bark oil and soybean oil on quality and microbiological safety of cantaloupe.the quality and microbiological safety of cantaloupes can potentially be improved using antimicrobial coatings that are able to maintain effectiveness throughout storage. the objective of this work was to study the effect of coating mixtures containing sodium alginate and cinnamon bark oil (cbo) on the quality of cantaloupes and the survival of inoculated bacterial pathogens and naturally occurring yeasts and molds during ambient storage at 21 °c. cantaloupes were dipped in mixtures containing 1 ...201526318410
listeria monocytogenes prevalence and characteristics in retail raw foods in china.the prevalence and levels of listeria monocytogenes in retail raw foods covering most provincial capitals in china were studied with testing of 1036 samples of vegetables, edible mushrooms, raw meat, aquatic products and quick-frozen products from september 2012 to january 2014. the total prevalence of listeria monocytogenes was 20.0% (207/1036), and the most probable number (mpn) values of 65.7% of the positive samples ranged from 0.3 to 110 mpn/g. geographical differences were observed in this ...201526317852
rhombencephalitis by listeria monocytogens in two diabetic patients.brainstem involvement is a characteristic feature and accounts for the high mortality associated with listeriosis especially in immunosuppressed patients. we report two cases of rhombencephalitis infection by listeria monocytogenes in a 65 and 63-year-old men with diabetes. they were referred to a neurologist due to fever and drowsiness after 3 weeks. the 65-year-old man had vertigo, diplopia, ataxia, bidirectional nystagmus and the 63-year-old man complained of perioral numbness, dysphagia and ...201526317605
pi3kδ regulates the magnitude of cd8+ t cell responses after challenge with listeria monocytogenes.pi3ks regulate diverse immune cell functions by transmitting intracellular signals from ag, costimulatory receptors, and cytokine receptors to control cell division, differentiation, survival, and migration. in this study, we report the effect of inhibiting the p110δ subunit of pi3kδ on cd8(+) t cell responses to infection with the intracellular bacteria listeria monocytogenes. a strong dependency on pi3kδ for ifn-γ production by cd8(+) t cells in vitro was not recapitulated after listeria infec ...201526311905
it is not all about single nucleotide polymorphisms: comparison of mobile genetic elements and deletions in listeria monocytogenes genomes links cases of hospital-acquired listeriosis to the environmental source.the control of food-borne outbreaks caused by listeria monocytogenes in humans relies on the timely identification of food or environmental sources and the differentiation of outbreak-related isolates from unrelated ones. this study illustrates the utility of whole-genome sequencing for examining the link between clinical and environmental isolates of l. monocytogenes associated with an outbreak of hospital-acquired listeriosis in sydney, australia. comparative genomic analysis confirmed an epid ...201526311854
evaluation of two loop-mediated isothermal amplification methods for the detection of salmonella enteritidis and listeria monocytogenes in artificially contaminated ready-to-eat fresh produce.in the present study, the effectiveness of two loop-mediated isothermal amplification (lamp) assays was evaluated. samples of romaine lettuce, strawberries, cherry tomatoes, green onions and sour berries were inoculated with known dilutions (10(0)-10(8) cfu/g of produce) of s. enteritidis and l. monocytogenes. with lamp, assay pathogens can be detected in less than 60 min. the limits of detection of s. enteritidis and l. monocytogenes depended on the food sample tested and on the presence of enr ...201527800413
a comparison analysis of listeria monocytogenes isolates recovered from chicken carcasses and human by using rapd pcr.chicken meat is a valuable source of protein and consumption of it continues to rise day to day. the aim of the present study was to evaluate the genetic homogeneity of listeria monocytogenes (l. monocytogenes) isolates obtained from chicken carcasses and human. random amplification of polymorphic dna (rapd) pcr with three different primers were used to analyze the 31 l. monocytogenes isolates recovered from human and chicken carcasses. primers were d8635, hlwl74, and opm01. scanned images of ra ...201526309714
influence of ph on bile sensitivity amongst various strains of listeria monocytogenes under aerobic and anaerobic conditions.listeria monocytogenes is a dangerous bacterium that causes the food-borne disease listeriosis and accounts for nearly 20% of food-borne deaths. this organism can survive the body's natural defences within the digestive tract, including acidic conditions and bile. although the bile response has been analysed, limited information is available concerning the ability of l. monocytogenes to resist bile under anaerobic conditions, especially at acidic ph, which mimics conditions within the duodenum. ...201526307079
sly1 regulates t-cell proliferation during listeria monocytogenes infection in a foxo1-dependent manner.infection of mice with listeria monocytogenes results in a strong t-cell response that is critical for an efficient defense. here, we demonstrate that the adapter protein sly1 (sh3-domain protein expressed in lymphocytes 1) is essential for the generation of a fully functional t-cell response. the lack of sly1 leads to reduced survival rates of infected mice. the increased susceptibility of sly1 knock-out (ko) mice was caused by reduced proliferation of differentiated t cells. ex vivo analyses o ...201526306874
quantification of listeria monocytogenes cells with digital pcr and their biofilm cells with real-time pcr.the purpose of this study was to develop a pcr-based method for quantification of listeria monocytogenes adhesion in microtitre plates. we optimized isolation of dna in the microtitre plates using cell lysis, ultrasound treatment, heating, and centrifugation of the lysate. digital pcr was applied for quantification of l. monocytogenes dna that was used for construction of the standard curve, and real-time pcr was used for quantification of the attached l. monocytogenes cells. this pcr-based meth ...201526304835
directly observing the lipid-dependent self-assembly and pore-forming mechanism of the cytolytic toxin listeriolysin o.listeriolysin o (llo) is the major virulence factor of listeria monocytogenes and a member of the cholesterol-dependent cytolysin (cdc) family. gram-positive pathogenic bacteria produce water-soluble cdc monomers that bind cholesterol-dependent to the lipid membrane of the attacked cell or of the phagosome, oligomerize into prepores, and insert into the membrane to form transmembrane pores. however, the mechanisms guiding llo toward pore formation are poorly understood. using electron microscopy ...201526302195
designer probiotics: development and applications in gastrointestinal health.given the increasing commercial and clinical relevance of probiotics, improving their stress tolerance profile and ability to overcome the physiochemical defences of the host is an important biological goal. herein, i review the current state of the art in the design of engineered probiotic cultures, with a specific focus on their utility as therapeutics for the developing world; from the treatment of chronic and acute enteric infections, and their associated diarrhoeal complexes, to targeting h ...201526301121
identification of listeria spp. strains isolated from meat products and meat production plants by multiplex polymerase chain reaction.listeriosis is a foodborne disease caused by listeria monocytogenes and is considered as a serious health problem, due to the severity of symptoms and the high mortality rate. recently, other listeria species have been associated with disease in human and animals. the aim of this study was to develop a multiplex polymerase chain reaction (pcr) in order to simultaneously detect six listeria species (l. grayi, l. welshimeri, l. ivanovii, l. monocytogenes, l. seeligeri, l. innocua) in a single reac ...201527800422
dietary effects of oregano (origanum vulgaris l.) plant or sweet chestnut (castanea sativa mill.) wood extracts on microbiological, chemico-physical characteristics and lipid oxidation of cooked ham during storage.the aim of this study was to evaluate the dietary effect of feeding pigs with diets enriched with sweet chestnut wood (castanea sativa mill.) or oregano (origanum vulgaris l.) extract on the microbiological and chemical characteristics of cooked pork ham. three groups of 10 pigs were fed with a control diet (ctrl), with the ctrl diet enriched with 0.2% of oregano extract (or) and with the ctrl diet enriched with 0.2% of sweet chestnut wood extract (scw), respectively. six cooked hams per group w ...201527800421
bacterial meningitis: an update of new treatment options.the outcome of bacterial meningitis critically depends on the rapid initiation of bactericidal antibiotic therapy and adequate management of septic shock. in community-acquired meningitis, the choice of an optimum initial empirical antibiotic regimen depends on the regional resistance patterns. pathogens resistant to antibacterials prevail in nosocomial bacterial meningitis. dexamethasone is recommended as adjunctive therapy for community-acquired meningitis in developed countries. in comatose p ...201526293166
sonorensin: a new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.the emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. in this study, sonorensin, predicted to belong to ...201526292786
evaluating the effect of copper nanoparticles in inhibiting pseudomonas aeruginosa and listeria monocytogenes biofilm formation.biofilm formation is a major virulence factor in different bacteria. biofilms allow bacteria to resist treatment with antibacterial agents. the biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases.201526290685
the padr-like transcriptional regulator lftr ensures efficient invasion of listeria monocytogenes into human host cells.invasion of the bacterial pathogen listeria monocytogenes into human host cells requires specialized surface molecules for attachment and induction of phagocytosis. however, efficient invasion is also dependent on factors with house-keeping functions, such as seca2-dependent secretion of autolysins for post-divisional segregation of daughter cells. mutations in this pathway prevent degradation of peptidoglycan cross-walls, so that long cell chains are formed that cannot be phagocytosed. the extr ...201526284051
experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.distinguishing between viable and dead bacteria in animal and urban effluents is a major challenge. among existing methods, propidium monoazide (pma)-qpcr is a promising way to quantify viable cells. however, its efficiency depends on the composition of the effluent, particularly on total suspended solids (tss)) and on methodological parameters. the aim of this study was evaluate the influence of three methodological factors (concentration of pma, incubation time and photoactivation time) on the ...201526276157
effect of rosemary essential oil and modified-atmosphere packaging (map) on meat quality and survival of pathogens in poultry fillets.the effect of rosemary (rosmarinus officinalis l.) essential oil (reo) and modified-atmosphere packaging (map) on the survival of certain pathogens (salmonella typhimurium and listeria monocytogenes) in poultry fillets and on their meat quality during 7 days of refrigerated storage were investigated. because reo at 0.05% and 0.1% had weak antibacterial activity and reo at 0.3%, 0.5% and 1.0% imparted unacceptable organoleptic properties, only reo at 0.2% was used to treat the poultry meat. the r ...201526273279
evaluation of 3m™ molecular detection assay (mda) listeria for the detection of listeria species in selected foods and environmental surfaces: collaborative study, first action 2014.06.the 3m™ molecular detection assay (mda) listeria is used with the 3m molecular detection system for the detection of listeria species in food, food-related, and environmental samples after enrichment. the assay utilizes loop-mediated isothermal amplification to rapidly amplify listeria target dna with high specificity and sensitivity, combined with bioluminescence to detect the amplification. the 3m mda listeria method was evaluated using an unpaired study design in a multilaboratory collaborati ...201526268982
evaluation of 3m™ molecular detection assay (mda) listeria monocytogenes for the detection of listeria monocytogenes in selected foods and environmental surfaces: collaborative study, first action 2014.07.the 3m™ molecular detection assay (mda) listeria monocytogenes combines isothermal amplification and bioluminescence to detect listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. the 3m mda listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the u.s. department of agriculture, food safety and inspection service microbiology laboratory guidebook 8.09 (2011) isolation and identific ...201526268981
nosocomial empyema caused by a rare serotype, serotype 4c, of listeria monocytogenes in a patient with rheumatoid arthritis and chronic kidney disease: a case report and review of the literature.the intracellular pathogen listeria monocytogenes presents characteristically as meningoencephalitis and bacteremia. herein, we report an extremely rare case of empyema caused by serotype 4c l. monocytogenes in an immunocompromised patient. this case supports the inclusion of l. monocytogenes infection in the differential diagnosis of empyema in an immunocompromised patient.201526268455
urban prevalence of listeria spp. and listeria monocytogenes in public lavatories and on shoe soles of facility patrons in the european capital city vienna.the aim of this study was to determine the prevalence of listeria spp. and listeria monocytogenes (l. monocytogenes) in urban public lavatories and on shoe soles of facility patrons in a european capital city. more than 91% of all municipal public lavatories in vienna close to public hubs were included in this study. overall, 373 swab samples of public lavatories and shoes of facility patrons were enriched, according to iso 11290-1. listeria monocytogenes isolates were subtyped using pulsed-fiel ...201524751465
pregnancy-associated listeriosis in england and wales.listeriosis is a rare but severe foodborne disease with low morbidity and high case-fatality rates. pregnant women, unborn and newborn babies are among the high-risk groups for listeriosis. we examined listeriosis cases reported to the enhanced surveillance system in england and wales from 1990 to 2010 to identify risk factors influencing outcome. cases were defined as pregnancy-associated if listeria monocytogenes was isolated from a pregnant woman or newborn infants aged <28 days. of the 3088 ...201524650375
occurrence, antibiotic resistance and molecular characterization of listeria monocytogenes in the beef chain in the republic of ireland.this study investigated the occurrence, concentration and key characteristics of listeria monocytogenes in beef chain samples (n=1100) over a 2-year period (july 2007-june 2009). listeria monocytogenes was isolated from bovine hides (27%), pre-chill carcasses (14%) and ground beef (29%), but not from ready-to-eat (rte) beef. the concentration of the pathogen in the majority (95%) of contaminated samples was low and detected by enrichment only. the highest concentrations recovered (100-200 cfu/g) ...201524506835
development and optimization of an egfp-based reporter for measuring the general stress response in listeria monocytogenes.a characteristic of the food-borne pathogen listeria monocytogenes is its tolerance to the harsh conditions found both in minimally processed foods and the human gastrointestinal tract. this trait is partly under the control of the alternative sigma factor sigma b (σ(b)). to study the mechanisms that trigger the activation of σ(b) , and hence the development of stress tolerance, we have developed a fluorescent reporter fusion that allows the real-time activity of σ(b) to be monitored. the report ...201522539028
[effect of volatile metabolites from germinating seeds on the reproduction of the bacteria listeria monocytogenes and yersinia pseudotuberculosis].the biological activity of volatile metabolites of germinating seeds of cabbage (brassica oleacia), carrot (daukus carota), salad (lactuca sativa), and corn (zea mays l.) against listeria monocytogenes and yersinia pseudotuberculosis was studied. it was shown that volatile metabolites are transfer factors and can be the sole carbon and energy source for these bacteria. methanol is the main substance affecting their growth and reproduction.201522834302
relating physicochemical and microbiological safety indicators during processing of linguiça, a portuguese traditional dry-fermented sausage.linguiça is a portuguese traditional fermented sausage whose microbiological quality and safety can be highly variable. in order to elucidate risk factors and the particularities of the manufacturing technology that explain the between-batch variability in total viable counts (tvc), enterobacteriaceae, staphylococcus aureus and listeria monocytogenes in the product; microbiological and physicochemical characterisation of linguiça at five stages of production (i.e., raw pork meat, mixed with ingr ...201528433317
the challenge of challenge testing to monitor listeria monocytogenes growth on ready-to-eat foods in europe by following the european commission (2014) technical guidance document.european regulation (ec) no. 2073/2005 lays down the microbiological criteria for certain microorganisms in foods and the implementing rules to be complied with by food business operators (fbos) in europe when implementing general and specific hygiene measures. in relation to listeria monocytogenes, this regulation covers primarily ready-to-eat (rte) food products, and requires different microbiological criteria depending on the ability of the food product to support growth of l. monocytogenes. ...201528454952
modeling the protective effect of aw and fat content on the high pressure resistance of listeria monocytogenes in dry-cured ham.high pressure processing (hpp) is a promising food preservation technology as an alternative to thermal processing for microbial inactivation. the technological parameters, the type of microorganism, and the food composition can greatly affect the microbicidal potential of hpp against spoilage and pathogenic microorganisms. presently, the number of available models quantifying the influence of food characteristics on the pathogen inactivation is scarce. the aim of this study was to model the ina ...201528454947
molecular characterization of silver-stearate nanoparticles (agstnps): a hydrophobic and antimicrobial material against foodborne pathogens.in this study, silver-stearate nanoparticles (agstnps) have been produced from silver nitrate solutions by replacing the nitrate by stearate ions and then reducing by thermal treatment. agstnps were characterized by particle size, zeta-potential, atr-ftir, contact angle and sem (scanning electron microscopy) analyses. the mean particle size and zeta potential of agstnps were determined to be 69.22±7.30nm and +8.02±0.88mv. atr-ftir analysis showed characteristic ir bands of stearate, revealing th ...201528455024
capability of exopolysaccharide-producing lactobacillus paraplantarum bgcg11 and its non-producing isogenic strain nb1, to counteract the effect of enteropathogens upon the epithelial cell line ht29-mtx.the putative protective role of the exopolysaccharide (eps)-producing lactobacillus paraplantarum bgcg11, and its non-eps-producing isogenic strain nb1, was tested upon ht29-mtx monolayers challenged with seven opportunistic pathogens. the probiotic strain lactobacillus rhamnosus lmg18243 (gg) was used as a reference bacterium. tested lactobacilli were able to efficiently reduce the attachment to ht29-mtx of most pathogens. lb. paraplantarum nb1 and lb. rhamnosus gg were more efficient reducing ...201528411984
bacterial attachment and biofilm formation on surfaces are reduced by small-diameter nanoscale pores: how small is small enough?prevention of biofilm formation by bacteria is of critical importance to areas that directly affect human health and life including medicine, dentistry, food processing and water treatment. this work showcases an effective and affordable solution for reducing attachment and biofilm formation by several pathogenic bacteria commonly associated with foodborne illnesses and medical infections.201528721236
sulfadiazine-chitosan conjugates and their polyelectrolyte complexes with hyaluronate destined to the management of burn wounds.in the present study polyelectrolyte complexes (pecs) based on new sulfadiazine-chitosan conjugates with sodium hyaluronate have been developed with potential use in treatment of burn wounds. the pecs were chemically characterized using fourier transform-infrared spectroscopy, scanning electon microscopy and near infrared chemical imaging technique. the swelling behavior and in vitro sulfadiazine release were also investigated. the antimicrobial activity was evaluated towards three bacterial str ...201528787940
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