Publications

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mechanistic model coupling gas exchange dynamics and listeria monocytogenes growth in modified atmosphere packaging of non respiring food.a mechanistic model coupling o2 and co2 mass transfer (namely diffusion and solubilisation in the food itself and permeation through the packaging material) to microbial growth models was developed aiming at predicting the shelf life of modified atmosphere packaging (map) systems. it was experimentally validated on a non-respiring food by investigating concomitantly the o2/co2 partial pressure in packaging headspace and the growth of listeria monocytogenes (average microbial count) within the fo ...201526187845
combined effects of thermosonication and slightly acidic electrolyzed water on the microbial quality and shelf life extension of fresh-cut kale during refrigeration storage.this study evaluated the efficacy of thermosonication combined with slightly acidic electrolyzed water (sacew) on the shelf life extension of fresh-cut kale during storage at 4 and 7 °c. each kale (10 ± 0.2 g) was inoculated to contain approximately 6 log cfu/g of listeria monocytogenes. each inoculated or uninoculated samples was dip treated at 40 °c for 3 min with deionized water, thermosonication (400 w/l), sacew (5 mg/l), sodium chlorite (sc; 100 mg/l), sodium hypochlorite (sh; 100 mg/l), an ...201526187840
microbiological assessment along the fish production chain of the norwegian pelagic fisheries sector--results from a spot sampling programme.microbes play an important role in the degradation of fish products, thus better knowledge of the microbiological conditions throughout the fish production chain may help to optimise product quality and resource utilisation. this paper presents the results of a ten-year spot sampling programme (2005-2014) of the commercially most important pelagic fish species harvested in norway. fish-, surface-, and storage water samples were collected from fishing vessels and processing factories. totally 1,1 ...201526187839
control of listeria species food safety at a poultry food production facility.surveillance and control of food-borne human pathogens, such as listeria monocytogenes, is a critical aspect of modern food safety programs at food production facilities. this study evaluated contamination patterns of listeria species at a poultry food production facility, and evaluated the efficacy of procedures to control the contamination and transfer of the bacteria throughout the plant. the presence of listeria species was studied along the production chain, including raw ingredients, food- ...201526187831
cold plasma treatment for the microbiological safety of cabbage, lettuce, and dried figs.microwave-powered cold plasma treatment (cpt) was evaluated as a means to improve the microbiological safety of fresh vegetables and dried fruits. the cpt at 900 w, conducted for 10 min using nitrogen as a plasma-forming gas, inactivated salmonella typhimurium inoculated on cabbage and lettuce by approximately 1.5 log cfu/g. the cpt at 400-900 w and 667 pa, conducted for 1-10 min using a helium-oxygen gas mixture, inactivated listeria monocytogenes on cabbage by 0.3-2.1 log cfu/g in a time-depen ...201526187830
comparative genomics of listeria sensu lato: genus-wide differences in evolutionary dynamics and the progressive gain of complex, potentially pathogenicity-related traits through lateral gene transfer.historically, genome-wide and molecular characterization of the genus listeria has concentrated on the important human pathogen listeria monocytogenes and a small number of closely related species, together termed listeria sensu strictu. more recently, a number of genome sequences for more basal, and nonpathogenic, members of the listeria genus have become available, facilitating a wider perspective on the evolution of pathogenicity and genome level evolutionary dynamics within the entire genus ...201526185097
cutting edge: a natural antisense transcript, as-il1α, controls inducible transcription of the proinflammatory cytokine il-1α.natural antisense transcripts (nats) are a class of long noncoding rnas (lncrnas) that are complementary to other protein-coding genes. although thousands of nats are encoded by mammalian genomes, their functions in innate immunity are unknown. in this study, we identified and characterized a novel nat, as-il1α, which is partially complementary to il-1α. similar to il-1α, as-il1α is expressed at low levels in resting macrophages and is induced following infection with listeria monocytogenes or s ...201526179904
prfa regulation offsets the cost of listeria virulence outside the host.virulence traits are essential for pathogen fitness, but whether they affect microbial performance in the environment, where they are not needed, remains experimentally unconfirmed. we investigated this question with the facultative pathogen listeria monocytogenes and its prfa virulence regulon. prfa-regulated genes are activated intracellularly (prfa 'on') but shut down outside the host (prfa 'off'). using a mutant prfa regulator locked on (prfa*) and thus causing prfa-controlled genes to be co ...201526178789
the multicopy srna lhrc controls expression of the oligopeptide-binding protein oppa in listeria monocytogenes.listeria monocytogenes is the causative agent of the foodborne disease listeriosis. during infection, l. monocytogenes produces an array of non-coding rnas, including the multicopy srna lhrc. these five, nearly identical srnas are highly induced in response to cell envelope stress and target the virulence adhesin lapb at the post-transcriptional level. here, we demonstrate that lhrc controls expression of additional genes encoding cell envelope-associated proteins with virulence function. using ...201526176322
a sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of listeria monocytogenes using an immobilization-free interdigitated array microelectrode.in this study, we described a novel impedance biosensor combining immunomagnetic separation with urease catalysis for sensitive detection of foodborne bacteria using listeria monocytogenes as model and an immobilization-free microelectrode as detector. the monoclonal antibodies (mabs) were immobilized on the surface of the magnetic nanoparticles (mnps) with the diameter of 180 nm by biotin-streptavidin system for specifically and efficiently separating listeria cells from sample background. the ...201526176211
unusual presentation of listerial myocarditis and the diagnostic value of cardiac magnetic resonance.listeria monocytogenes is an infrequent cause of bacterial myocarditis. myocarditis without evidence of endocarditis is even rarer. management in such cases involves early diagnosis, antibiotic therapy, and emergency treatment of arrhythmias. we report the case of a 47-year-old man who presented with features of acute st-segment-elevation myocardial infarction complicated by ventricular tachycardia that necessitated urgent electrical cardioversion. contrast-enhanced cardiac magnetic resonance im ...201526175642
application of a novel antimicrobial coating on roast beef for inactivation and inhibition of listeria monocytogenes during storage.the antilisterial efficacy of novel coating solutions made with organic acids, lauric arginate ester, and chitosan was evaluated in a three-stage study on inoculated roast beef for the first time. ready-to-eat roast beef was specially ordered from the manufacturer. the meat surface was inoculated with five-strain listeria monocytogenes cocktail inoculums at two different levels, ~3 and 6 log cfu/cm(2) and treated with the stock solution (hams), the 1:5 diluted solution (mams), and the 1:10 dilut ...201526173201
lamellipodin is important for cell-to-cell spread and actin-based motility in listeria monocytogenes.listeria monocytogenes is a foodborne pathogen capable of invading a broad range of cell types and replicating within the host cell cytoplasm. this paper describes the colocalization of host cell lamellipodin (lpd) with intracellular l. monocytogenes detectable 6 h postinfection of epithelial cells. the association was mediated via interactions between both the peckstrin homology (ph) domain in lpd and phosphatidylinositol (3,4)-bisphosphate [pi(3,4)p2] on the bacterial surface and by interactio ...201526169271
fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens.low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ...201526162872
occurrence of listeria monocytogenes in ready-to-eat meat products and meat processing plants in spain.the aim of this work was to study the occurrence of listeria monocytogenes in several types of ready-to-eat (rte) meat products and in the environment of meat processing plants. a total of 129 samples of rte meat products and 110 samples from work surfaces and equipment were analyzed. l. monocytogenes was detected in 6 out of 35 cooked products (17.14%), 21 out of 57 raw-cured products (36.84%), and 9 out of 37 dry-cured, salted products (24.32%). the number of sample units that exceeded the foo ...201528231204
spontaneous restoration of transplantation tolerance after acute rejection.transplantation is a cure for end-stage organ failure but, in the absence of pharmacological immunosuppression, allogeneic organs are acutely rejected. such rejection invariably results in allosensitization and accelerated rejection of secondary donor-matched grafts. transplantation tolerance can be induced in animals and a subset of humans, and enables long-term acceptance of allografts without maintenance immunosuppression. however, graft rejection can occur long after a state of transplantati ...201526151823
age-related gene expression differences in monocytes from human neonates, young adults, and older adults.a variety of age-related differences in the innate and adaptive immune systems have been proposed to contribute to the increased susceptibility to infection of human neonates and older adults. the emergence of rna sequencing (rna-seq) provides an opportunity to obtain an unbiased, comprehensive, and quantitative view of gene expression differences in defined cell types from different age groups. an examination of ex vivo human monocyte responses to lipopolysaccharide stimulation or listeria mono ...201526147648
cooperative transcriptional activation of antimicrobial genes by stat and nf-κb pathways by concerted recruitment of the mediator complex.the transcriptional response to infection with the bacterium listeria monocytogenes (lm) requires cooperative signals of the type i interferon (ifn-i)-stimulated jak-stat and proinflammatory nf-κb pathways. using chip-seq analysis, we define genes induced in lm-infected macrophages through synergistic transcriptional activation by nf-κb and the ifn-i-activated transcription factor isgf3. using the nos2 and il6 genes as prime examples of this group, we show that nf-κb functions to recruit enzymes ...201526146080
characterization of specific alleles in inla and prfa of listeria monocytogenes isolated from foods in osaka, japan and their ability to invade caco-2 cells.listeria monocytogenes expresses the surface protein internalin a (inla), enabling the invasion of human intestinal epithelial cells to cause severe food-borne diseases. full-length sequence analysis of inla of 114 food isolates resulted in the detection of 29 isolates with a premature stop codon (pmsc) mutation and 6 isolates with 3-codon deletion mutations (aa 738 to 740) in inla. the isolates with inla pmscs demonstrated a significantly lower level of invasion than the other food isolates in ...201526143289
increased spread and replication efficiency of listeria monocytogenes in organotypic brain-slices is related to multilocus variable number of tandem repeat analysis (mlva) complex.listeria (l.) monocytogenes causes fatal infections in many species including ruminants and humans. in ruminants, rhombencephalitis is the most prevalent form of listeriosis. using multilocus variable number tandem repeat analysis (mlva) we recently showed that l. monocytogenes isolates from ruminant rhombencephalitis cases are distributed over three genetic complexes (designated a, b and c). however, the majority of rhombencephalitis strains and virtually all those isolated from cattle cluster ...201526138984
myeloid sirtuin 2 expression does not impact long-term mycobacterium tuberculosis control.sirtuins (sirts) regulate several cellular mechanisms through deacetylation of several transcription factors and enzymes. recently, sirt2 was shown to prevent the development of inflammatory processes and its expression favors acute listeria monocytogenes infection. the impact of this molecule in the context of chronic infections remains unknown. we found that specific sirt2 deletion in the myeloid lineage transiently increased mycobacterium tuberculosis load in the lungs and liver of conditiona ...201526135889
defining and evaluating a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of listeria monocytogenes.whole-genome sequencing (wgs) has emerged today as an ultimate typing tool to characterize listeria monocytogenes outbreaks. however, data analysis and interlaboratory comparability of wgs data are still challenging for most public health laboratories. therefore, we have developed and evaluated a new l. monocytogenes typing scheme based on genome-wide gene-by-gene comparisons (core genome multilocus the sequence typing [cgmlst]) to allow for a unique typing nomenclature. initially, we determined ...201526135865
using the chicken embryo to assess virulence of listeria monocytogenes and to model other microbial infections.microbial infections are a global health problem, particularly as microbes are continually developing resistance to antimicrobial treatments. an effective and reliable method for testing the virulence of different microbial pathogens is therefore a useful research tool. this protocol describes how the chicken embryo can be used as a trustworthy, inexpensive, ethically desirable and quickly accessible model to assess the virulence of the human bacterial pathogen listeria monocytogenes, which can ...201526134955
listeria meningitis complicating alemtuzumab treatment in multiple sclerosis--report of two cases.alemtuzumab, a humanized monoclonal antibody targeting the surface molecule cd52, leads to a rapid depletion of immune cells in the innate and adaptive immune system. in phase 2 and 3 trials in multiple sclerosis (ms), infections have been reported more frequently in alemtuzumab than in interferon beta treated patients. here we report two patients treated with alemtuzumab for ms developing listeria meningitis few days after the first infusion cycle. both patients recovered completely after promp ...201526132570
solitary supratentorial listeria monocytogenes brain abscess in an immunocompromised patient.we describe an 81-year-old man receiving azacitidine monotherapy for myelodysplastic syndrome who was improving from listeria monocytogenes bacteremia after receiving antibiotic therapy during an earlier hospital admission. shortly after discharge he developed new-onset seizure activity, with brain imaging on subsequent admissions demonstrating a posterior right frontal lobe mass. specimen cultures after resection of the mass revealed this to be a cerebral abscess related to l. monocytogenes. br ...201526130881
different types of stainless steel used in equipment in meat plants do not affect the initial microbial transfer, including pathogens, from pork skin.this study describes and measures the impact of different compositions and finishes of stainless steel used in equipment in the meat industry on the transfer of natural flora and selected pathogens from artificially contaminated pork skin. it is known that the adhesion to surfaces of listeria monocytogenes and salmonella, 2 pathogens frequently found in contaminated pork meat, depends on the nature and roughness of the surface. our results show no statistically significant differences in microbi ...201526130860
diadenylate cyclase evaluation of ssdaca (ssu98_1483) in streptococcus suis serotype 2.cyclic diadenosine monophosphate is a recently identified signaling molecule. it has been shown to play important roles in bacterial pathogenesis. ssu98_1483 (ssdaca), which is an ortholog of listeria monocytogenes daca, is a putative diadenylate cyclase in streptococcus suis serotype 2. in this study, we determined the enzymatic activity of ssdaca in vitro using high-performance liquid chromatography and mass spectrometry. our results showed that ssdaca was a diadenylate cyclase that converts a ...201526125899
ultraviolet-c light inactivation of escherichia coli o157:h7 and listeria monocytogenes on organic fruit surfaces.this study investigated uv-c light inactivation of escherichia coli o157:h7 and listeria monocytogenes on the surface of organic apples, pears, strawberries, red raspberries and cantaloupes. fruit surfaces spot inoculated with cocktail strains of e. coli o157:h7 and l. monocytogenes were exposed to uv-c doses up to 11.9 kj/m(2) at 23 °c. fruit surface roughness, contact angle, and surface energy were determined and correlated with uv-c inactivation kinetics. results demonstrate that bacterial pa ...201526122954
rescue of cd8+ t cell vaccine memory following sublethal γ irradiation.sublethal γ irradiation eliminates cd8+ t cell mediated memory responses. in this work, we explored how these memory responses could be rescued in the aftermath of such exposure. we utilized two models of cd8+ t cell mediated immunity: a mouse model of listeria monocytogenes (lm) infection in which cd8+ t cells specific for lm expressed antigens (listeriolysin o, llo) can be tracked, and a murine skin graft model in which cd8+ t cells mediate rejection across a mhc class i (d(d)) disparity. in t ...201526122582
stability, compatibility and microbiological activity studies of meropenem-clavulanate potassium.meropenem (mem) and clavulanate potassium have been reported to demonstrate highly effective activity against mycobacterium tuberculosis. there have been no reports on research into the complex of these chemotherapeutics concerning their mutually dependent stability or microbiological action on other microorganisms. stability and compatibility studies of mem/clavulanate potassium were conducted by using an hplc-dad method. the antibacterial activity of mem/clavulanate potassium was tested in vit ...201525074659
enhanced inactivation of food-borne pathogens in ready-to-eat sliced ham by near-infrared heating combined with uv-c irradiation and mechanism of the synergistic bactericidal action.the objective of the study described in this article was, first, to investigate the effect of the simultaneous application of near-infrared (nir) heating and uv irradiation on inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat (rte) sliced ham and as well as its effect on product quality and, second, to elucidate the underlying mechanisms of the synergistic bactericidal action of nir heating and uv irradiation. with the i ...201525107964
identification, characterization, immobilization of a novel type hydrolase (lmh) from listeria monocytogenes.a novel type of hydrolase (lmh) from listeria monocytogenes was identified, characterized, and immobilized for biotechnological applications. primary sequence analysis indicated that lmh had a catalytic triad (ser(91)-asp(192)-his(222)) with a molecular weight of 27.8 kda. homologs of this enzyme are produced by many gram-positive bacteria including bacillus, staphylococcus, and enterococcus. biochemical properties of lmh were investigated by performing mass spectrometry, dynamic light scatterin ...201525128099
progression to multi-scale models and the application to food system intervention strategies.the aim of this article is to discuss how the systems science approach can be used to optimize intervention strategies in food animal systems. it advocates the idea that the challenges of maintaining a safe food supply are best addressed by integrating modeling and mathematics with biological studies critical to formulation of public policy to address these challenges. much information on the biology and epidemiology of food animal systems has been characterized through single-discipline methods ...201525217407
the p60 and nama autolysins from listeria monocytogenes contribute to host colonization and induction of protective memory.inducing long-term protective memory cd8(+) t-cells is a desirable goal for vaccines against intracellular pathogens. however, the mechanisms of differentiation of cd8(+) t-cells into long-lived memory cells capable of mediating protection of immunized hosts remain incompletely understood. we have developed an experimental system using mice immunized with wild type (wt) or mutants of the intracellular bacterium listeria monocytogenes (lm) that either do or do not develop protective memory cd8(+) ...201525225110
listeria monocytogenes isolated from food samples from a romanian black market show distinct virulence profiles.listeria monocytogenes is a facultative intracellular foodborne pathogen responsible for listeriosis. in a recent study, in which we investigated neglected exogenous routes of transmission of foodborne pathogens into the european union, we have isolated 15 l. monocytogenes strains in food products, which were imported from the republic of moldavia to romania and illegally sold at a local market. the aim of this study was to characterize the subtype and virulence potential of these 15 l. monocyto ...201525241012
fisetin inhibits listeria monocytogenes virulence by interfering with the oligomerization of listeriolysin o.listeriolysin o (llo), an essential virulence determinant of listeria monocytogenes, is a pore-forming toxin whose primary function is to facilitate cytosolic bacterial replication by breaching the phagosomal membranes, which is critical for the pathogen to evade host immune recognition. the critical role of llo in the virulence of l. monocytogenes renders it an ideal target for designing novel antivirulence therapeutics. we found that fisetin, a natural flavonoid without antimicrobial activity, ...201525231018
old war, new battle, new fighters! 201525231016
preparation of a porcine plasma protein composite film and its application.to use blood released from slaughtering houses, a porcine plasma protein (ppp)/nanoclay composite film was prepared. the tensile strength and elongation at break values of the ppp composite film with 5% nanoclay were 10.01 mpa and 6.55%, respectively. the ppp composite film containing 1% grapefruit seed extract (gse) was applied to pork meat, and the populations of inoculated escherichia coli o157:h7 and listeria monocytogenes in the pork meat packaged with the ppp composite film decreased by 0. ...201525248798
a novel function of interferon regulatory factor-1: inhibition of th2 cells by down-regulating the il4 gene during listeria infection.infection with certain pathogens induces a shift of the th subset balance to a th1 dominant state. this, in turn, results in the suppression of th2 responses. we focused on the involvement of interferon regulatory factor-1 (irf-1) in the suppression of th2 cells during listeria infection. we found that the inhibition of il-4 production by th2 cells is mediated by a soluble factor (lmsn) produced by listeria-infected antigen-presenting cells. the inhibition is not observed with t cells from irf1 ...201525280793
lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines.internalins comprise a class of listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. in this paper, a possible role of lmo0171-a new member of the internalin family was investigated. disruption of the lmo0171 gene resulted in important cell morphology alterations along with a decrease in the ability to invade three eukaryotic cell lines, that is int407, hep-2 and hela and diminished adhesion efficiency t ...201525323012
the diaphanous-related formins promote protrusion formation and cell-to-cell spread of listeria monocytogenes.the gram-positive bacterium listeria monocytogenes is a facultative intracellular pathogen whose virulence depends on its ability to spread from cell to cell within an infected host. although the actin-related protein 2/3 (arp2/3) complex is necessary and sufficient for listeria actin tail assembly, previous studies suggest that other actin polymerization factors, such as formins, may participate in protrusion formation. here, we show that arp2/3 localized to only a minor portion of the protrusi ...201525281757
species-specific engagement of human nucleotide oligomerization domain 2 (nod)2 and toll-like receptor (tlr) signalling upon intracellular bacterial infection: role of crohn's associated nod2 gene variants.recognition of bacterial peptidoglycan-derived muramyl-dipeptide (mdp) by nucleotide oligomerization domain 2 (nod2) induces crucial innate immune responses. most bacteria carry the n-acetylated form of mdp (a-mdp) in their cell membranes, whereas n-glycolyl mdp (g-mdp) is typical for mycobacteria. experimental murine studies have reported g-mdp to have a greater nod2-stimulating capacity than a-mdp. as nod2 polymorphisms are associated with crohn's disease (cd), a link has been suggested betwee ...201525335775
an essential role of a ferritin-like protein in acid stress tolerance of listeria monocytogenes.the expression of ten genes of listeria monocytogenes previously identified as penicillin g-inducible was transcriptionally analyzed in the presence of 0.5 m kcl, ph 5.0 and 42 °c. this study revealed that all the genes are upregulated by osmotic stress, seven by acid stress and four by temperature stress conditions. the contribution of a gene encoding a ferritin-like protein (fri), a two-component phosphate-response regulator (phop) and an arac/xyls family transcription regulator (axyr) to temp ...201525352185
visualization of granzyme b-expressing cd8 t cells during primary and secondary immune responses to listeria monocytogenes.cd8 t cells contribute to long-term protection against listeria monocytogenes infection by differentiating into memory t cells. these rapidly respond to antigen or inflammation upon secondary infection. in this study we used cd8 t cells from ot1 mice and cd4 t cells from ot2 mice expressing a fluorescent chimeric granzyme (gzmb-tom) protein to monitor the primary response to infection with ovalbumin-expressing l. monocytogenes (lm-ova). we show that, unlike poorly responding cd4 t cells, cd8 t c ...201525367158
depletion of autophagy-related genes atg3 and atg5 in tenebrio molitor leads to decreased survivability against an intracellular pathogen, listeria monocytogenes.macroautophagy (autophagy) is an evolutionarily conserved catabolic process involved in physiological and developmental processes including cell survival, death, and innate immunity. homologues of most of 36 originally discovered autophagy-related (atg) genes in yeast have been characterized in higher eukaryotes including insects. in this study, the homologues of atg3 (tmatg3) and atg5 (tmatg5) were isolated from the coleopteran beetle, tenebrio molitor by expressed sequence tag and rnaseq appro ...201525403020
acute prostatitis probably due to listeria monocytogenes in an hiv-infected patient.we report a unique case of acute bacterial prostatitis probably caused by listeria monocytogenes in an hiv-infected patient. for the best of our knowledge, this is the first case reported of a patient with this association. our case illustrates the protean clinical presentations that l. monocytogenes infections may adopt, particularly in immunocompromised patients.201525411351
role of the twin-arginine translocase (tat) system in iron uptake in listeria monocytogenes.the twin-arginine translocase (tat) complex is a unique system that translocates folded proteins across the cytoplasmic membrane. in this study, the tat transporter system in listeria monocytogenes was characterized to determine the role of tat in the iron uptake pathway. a putative tatac operon, containing conserved fur-binding sequences in the promoter region, has been predicted to encode tat-translocase components. another operon, fepcab, with a putative fur-binding sequence in the promoter, ...201525416690
[99mtc]annexin v-128 spect monitoring of splenic and disseminated listeriosis in mice: a model of imaging sepsis.here, we evaluate [(99m)tc]annexin v-128, an in vivo marker of apoptosis, for single photon emission computed tomography (spect) imaging of localization and antibiotic treatment of disseminated bacterial infection, using a well-described mouse model that employs bioluminescent listeria monocytogenes.201525416406
discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of listeria monocytogenes.upon ingestion of contaminated food, listeria monocytogenes can cause serious infections in humans that are normally treated with β-lactam antibiotics. these target listeria's five high molecular weight penicillin-binding proteins (hmw pbps), which are required for peptidoglycan biosynthesis. the two bi-functional class a hmw pbps pbp a1 and pbp a2 have transglycosylase and transpeptidase domains catalyzing glycan chain polymerization and peptide cross-linking, respectively, whereas the three cl ...201525424554
the listeria monocytogenes hibernation-promoting factor is required for the formation of 100s ribosomes, optimal fitness, and pathogenesis.during exposure to certain stresses, bacteria dimerize pairs of 70s ribosomes into translationally silent 100s particles in a process called ribosome hibernation. although the biological roles of ribosome hibernation are not completely understood, this process appears to represent a conserved and adaptive response that contributes to optimal survival during stress and post-exponential-phase growth. hibernating ribosomes are formed by the activity of one or more highly conserved proteins; gammapr ...201525422304
transcriptional response of selected genes of salmonella enterica serovar typhimurium biofilm cells during inactivation by superheated steam.superheated steam (shs), produced by the addition of heat to saturated steam (ss) at the same pressure, has great advantages over conventional heat sterilization due to its high temperature and accelerated drying rate. we previously demonstrated that treatment with shs at 200°c for 10 sec inactivated escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes biofilm cells on the surface of stainless steel to below the detection limit. however, bacteria withstanding heat stress ...201525440555
fast and discriminative cosyps detection system of viable salmonella spp. and listeria spp. in carcass swab samples.in this study, the complete cosyps path food workflow including all steps, namely swab sample enrichment, sybr®green qpcr detection of salmonella spp. and listeria spp., isolation and confirmation of the detected strain, was validated on beef carcass swabs. to perform the validation, the results of the complete workflow were compared, according to the iso 16140:2003, with the iso reference methods for detection, isolation and confirmation of listeria monocytogenes and salmonella spp. the results ...201525440553
subversion of the cytoskeleton by intracellular bacteria: lessons from listeria, salmonella and vibrio.entry into host cells and intracellular persistence by invasive bacteria are tightly coupled to the ability of the bacterium to disrupt the eukaryotic cytoskeletal machinery. herein we review the main strategies used by three intracellular pathogens to harness key modulators of the cytoskeleton. two of these bacteria, namely listeria monocytogenes and salmonella enterica serovar typhimurium, exhibit quite distinct intracellular lifestyles and therefore provide a comprehensive panel for the under ...201525440316
evaluation of dna extraction methods for pcr-based detection of listeria monocytogenes from vegetables.epidemiological data indicate that raw vegetables are associated with outbreaks of listeria monocytogenes. therefore, there is a demand for the availability of rapid and sensitive methods, such as pcr assays, for the detection and accurate discrimination of l. monocytogenes. however, the efficiency of pcr methods can be negatively affected by inhibitory compounds commonly found in vegetable matrices that may cause false-negative results. therefore, the sample processing and dna isolation steps m ...201525431169
high affinity anti-internalin b vhh antibody fragments isolated from naturally and artificially immunized repertoires.the need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. furthermore, distribution of tainted food and water can have consequences which can affect whole economies. antibodies and antibody fragments have been historically used in detection platforms due to their antigen specificity and robust physicochemical properties. in this study, we report the isolation and characterization of antibody fragm ...201525450000
a novel method to detect listeria monocytogenes via superparamagnetic lateral flow immunoassay.a novel strip test system combining immunomagnetic separation with lateral flow immunoassay (lfia) was established for the accurate detection of listeria monocytogenes. in this system, a pair of matched monoclonal antibodies was used to construct a sandwich immunoassay, in which superparamagnetic particles were coupled with one of the antibodies as a labeled antibody to capture the target bacteria, while the other antibody was immobilized on the detection zone. after a 20-min reaction, the strip ...201525486917
attached and planktonic listeria monocytogenes global proteomic responses and associated influence of strain genetics and temperature.contamination of industrial and domestic food usage environments by the attachement of bacterial food-borne pathogen listeria monocytogenes has public health and economic implications. comprehensive proteomics experiments using label-free liquid chromatography/tandem mass spectrometry were used to compare the proteomes of two different l. monocytogenes strains (siliken_1/2c and f2365_4b), which show very different capacities to attach to surfaces. growth temperature and strain type were highly i ...201525516393
inactivation of pathogenic listeria monocytogenes in raw milk by high hydrostatic pressure.the aim of this work was to investigate the mechanisms underlying the inactivation of listeria monocytogenes in raw milk under high hydrostatic pressure (hhp). the results showed that a 450-mpa treatment for 5 min could completely eliminate 10⁸ colony-forming units/ml of viable bacteria, as indicted by increased uptake of propidium iodide. morphological damage to the cell wall, cell membrane, and cytoplasmic components by hhp treatments was observed on scanning and transmission electron microgra ...201525514674
simultaneous construction of pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps.the aim of this study was to simultaneously construct pcr-dgge-based predictive models of listeria monocytogenes and vibrio parahaemolyticus on cooked shrimps at 4 and 10°c. calibration curves were established to correlate peak density of dgge bands with microbial counts. microbial counts derived from pcr-dgge and plate methods were fitted by baranyi model to obtain molecular and traditional predictive models. for l. monocytogenes, growing at 4 and 10°c, molecular predictive models were construc ...201525470339
cxcr3 expression defines a novel subset of innate cd8+ t cells that enhance immunity against bacterial infection and cancer upon stimulation with il-15.innate cd8(+) t cells are a heterogeneous population with developmental pathways distinct from conventional cd8(+) t cells. however, their biology, classification, and functions remain incompletely understood. we recently demonstrated the existence of a novel population of chemokine (c-x-c motif) receptor 3 (cxcr3)-positive innate cd8(+) t cells. here, we investigated the functional properties of this subset and identified effector molecules and pathways which mediate their function. adoptive tr ...201525466888
microbiological detection of bacteria in animal products seized in baggage of international air passengers to brazil.airline travel favours the transmission of diseases, given the short time it takes to travel long distances. in this study, animal products without health certificates seized in international air passengers' baggage at guarulhos (gru) and galeão (gig) airports in brazil underwent a microbiological evaluation. analyses (1610) were carried out on 322 seizures to test for the presence of total and thermotolerant coliforms, as well as staphylococcus aureus counts and the presence of listeria monocyt ...201525466683
quantifying variability on thermal resistance of listeria monocytogenes.knowledge of the impact of strain variability and growth history on thermal resistance is needed to provide a realistic prediction and an adequate design of thermal treatments. in the present study, apart from quantifying strain variability on thermal resistance of listeria monocytogenes, also biological variability and experimental variability were determined to prioritize their importance. experimental variability was defined as the repeatability of parallel experimental replicates and biologi ...201525462932
identification of non-listeria spp. bacterial isolates yielding a β-d-glucosidase-positive phenotype on agar listeria according to ottaviani and agosti (aloa).agar listeria according to ottaviani and agosti (aloa) is the mandatory medium used for the detection and enumeration of listeria monocytogenes in foods according to the official international organization for standardization (iso) methods. on aloa, listeria spp. appear as bluish-green colonies due to the production of β-d-glucosidase, an enzyme that cleaves 5-bromo-4-chloro-3-indolyl-β-d-glucopyranoside, a chromogenic substrate included in the formulation of the medium. the present work reports ...201525462931
gold nanoparticle-based dynamic light scattering immunoassay for ultrasensitive detection of listeria monocytogenes in lettuces.gold nanoparticle (gnp)-based dynamic light scattering (dls) assay has been widely used for sensitive detection of small analytes based on analyte binding-induced gnp aggregation. however, the use of this new method to detect large biological objectives, such as pathogenic bacteria, has not been reported. this study is the first to describe a homogeneous gnp-based dls immunoassay for ultrasensitive detection of listeria monocytogenes. compared with small analytes, l. monocytogenes has a larger s ...201525460900
estimating the non-thermal inactivation of listeria monocytogenes in fermented sausages relative to temperature, ph and water activity.data relative to in situ listeria monocytogenes inactivation in fermented sausages were collected from 13 individual studies found in the literature. inactivation rates were extrapolated and used to develop a predictive model to evaluate the relative effects of ph, water activity (a(w)) and temperature on l. monocytogenes fate during fermentation and ripening. temperature explained ca. 60% of the data variability, while ph and a(w) only a small part. temperature alone may not be sufficient to ca ...201525460122
identification and molecular characterization of pathogenic bacteria in foods confiscated from non-eu flights passengers at one spanish airport.two hundred food samples of animal origin confiscated from passengers arriving on flights from non-european countries at the international airport of bilbao (spain) were tested for the presence of four main bacterial foodborne pathogens (campylobacter spp., escherichia coli o157:h7, listeria monocytogenes and salmonella spp.) during 2012 and 2013. overall, 20 samples were positive for l. monocytogenes (10%) and 11 for salmonella spp. (5.5%), whereas campylobacter spp. and e. coli o157:h7 were no ...201525454793
enhanced transport of nanocage stabilized pure nanodrug across intestinal epithelial barrier mimicking listeria monocytogenes.ligand grafted nanoparticles have been shown to enhance drug transport across epithelium barrier and are expected to improve drug delivery. however, grafting of these ligands to the surface of pure nanodrug, i.e., nanocrystals (ncs), is a critical challenge due to the shedding of ligands along with the stabilizer upon high dilution or dissolving of the drug. herein, a non-sheddable nanocage-like stabilizer was designed by covalent cross-linking of poly(acrylic acid)-b-poly(methyl acrylate) on dr ...201525453961
synergistic effect of gamma (γ)-irradiation and microencapsulated antimicrobials against listeria monocytogenes on ready-to-eat (rte) meat.oregano essential oil (origanum compactum; 250 μg/ml), cinnamon essential oil (cinnamomum cassia; 250 μg/ml) and nisin (16 μg/ml) were used alone or in combination to evaluate their efficiency to inhibit the growth of listeria monocytogenes on rte ham. microencapsulation of the antimicrobial formulations was done to verify the potential effect of the polymer to protect the antimicrobial efficiency during storage. combined treatments of antimicrobial formulation with γ-irradiation were done to ve ...201525475322
role of sigb and osmolytes in desiccation survival of listeria monocytogenes in simulated food soils on the surface of food grade stainless steel.this research aimed to determine whether the sigb (σ(b)) regulon and osmolytes impact the survival of the foodborne pathogen, listeria monocytogenes, during desiccation in simulated food soils with varying salt and nutrient contents on food grade stainless steel (ss) surfaces. l. monocytogenes 568 (lm568, serotype 1/2a), its isogenic sigb mutant (δsigb) and the back-complemented δsigb were desiccated in bhi, tsb with 1% glucose (tsb-glu), peptone physiological saline (pps) and minimal media (mm) ...201525475314
differential gene expression profiling of listeria monocytogenes in cacciatore and felino salami to reveal potential stress resistance biomarkers.the current study reports a) the in situ transcriptional profiles of listeria monocytogenes in response to fermented sausage stress and b) an approach in which in situ rt-qpcr data have been combined with advanced statistical techniques to discover potential stress resistance or cell viability biomarkers. gene expression profiling of the pathogen has been investigated using rt-qpcr to understand how l. monocytogenes responds to the conditions encountered during the fermentation and ripening of s ...201525475310
environmental and biological factors influencing the uv-c resistance of listeria monocytogenes.in this investigation, the effect of microbiological factors (strain, growth phase, exposition to sublethal stresses, and photorepair ability), treatment medium characteristics (ph, water activity, and absorption coefficient), and processing parameters (dose and temperature) on the uv resistance of listeria monocytogenes was studied. the dose to inactivate 99.99% of the initial population of the five strains tested ranged from 21.84 j/ml (stcc 5672) to 14.66 j/ml (stcc 4031). the uv inactivation ...201525475293
effect of quantity of food residues on resistance to desiccation of food-related pathogens adhered to a stainless steel surface.in order to study the effect of food residues on the survival of food-borne pathogens, salmonella typhimurium, staphylococcus aureus, and listeria monocytogenes were subjected to drying conditions in the presence of small amounts of food such as carrot juice, aqueous solution of nori, milk, and soy-milk. after drying for 2 h at room temperature in the absence of food residue, cell counts of s. typhimurium, s. aureus, and l. monocytogenes decreased from 8 to 3, 6, and 5 log cfu/dish, respectively ...201525475291
effects of osmotic pressure, acid, or cold stresses on antibiotic susceptibility of listeria monocytogenes.prevalence of antibiotic resistance of listeria monocytogenes isolated from a variety of foods has increased in many countries. l. monocytogenes has many physiological adaptations that enable survival under a wide range of environmental stresses. the objective of this study was to evaluate effects of osmotic (2, 4, 6, 12% nac), ph (6, 5.5, 5.0) and cold (4 °c) stresses on susceptibility of three isolates of l. monocytogenes towards different antibiotics. the minimal inhibitory concentrations (mi ...201525475279
flexible thin-layer dielectric barrier discharge plasma treatment of pork butt and beef loin: effects on pathogen inactivation and meat-quality attributes.the effects of a flexible thin-layer dielectric barrier discharge (dbd) plasma system using a sealed package on microbial inactivation and quality attributes of fresh pork and beef were tested. following a 10-min treatment, the microbial-load reductions of listeria monocytogenes, escherichia coli o157:h7, and salmonella typhimurium were 2.04, 2.54, and 2.68 log cfu/g in pork-butt samples and 1.90, 2.57, and 2.58 log cfu/g in beef-loin samples, respectively. colorimetric analysis showed that dbd- ...201525475266
listeria monocytogenes dose response revisited--incorporating adjustments for variability in strain virulence and host susceptibility.evaluations of listeria monocytogenes dose-response relationships are crucially important for risk assessment and risk management, but are complicated by considerable variability across population subgroups and l. monocytogenes strains. despite difficulties associated with the collection of adequate data from outbreak investigations or sporadic cases, the limitations of currently available animal models, and the inability to conduct human volunteer studies, some of the available data now allow r ...201524975545
definition of a standard protocol to determine the growth potential of listeria monotgenes and yersinia enterocolitica in pork sausage produced in abruzzo region, italy.pork meat products consumed raw or after a short period of fermentation can be considered at risk for food safety. sausages (fresh sausage made from pork meat) are produced in several italian regions, with variation in ingredients. in some italian regions, including abruzzo, these products are frequently consumed raw or undercooked, after a variable period of fermentation. the european community food regulation promotes the use of challenge tests to determine safety levels. this study is aimed t ...201527800415
listeria monocytogenes infection in a sugar glider (petaurus breviceps) - new mexico, 2011.listeria monocytogenes is a gram-positive, facultative anaerobic, rod-shaped bacterium that can infect and cause disease in many species. in this case report, we describe a case of l. monocytogenes infection causing sepsis in a sugar glider (petaurus breviceps). the sugar glider consumed a varied diet consisting of human food items, including cantaloupe. a nationwide outbreak of l. monocytogenes foodborne illness associated with cantaloupes occurred simultaneously with this incident case. in thi ...201524815369
antimicrobial susceptibility of listeria monocytogenes isolates from human cases in northern italy, 2008-2010: mic determination according to eucast broth microdilution method.susceptibility of 96 listeria monocytogenes human isolates collected in northern italy between 2008 and 2010, to 15 antimicrobials, was investigated. minimum inhibitory concentration (mic) was evaluated by means of the standardized broth microdilution method, according to the european committee on antimicrobial susceptibility testing (eucast) international guidelines. all l. monocytogenes human isolates were susceptible to penicillin g (mic(90)≤0·06 μg/ml), meropenem (mic(90)≤0·06 μg/ml), and er ...201524720267
technologies and mechanisms for safety control of ready-to-eat muscle foods: an updated review.ready-to-eat (rte) muscle foods refer to a general category of meat and poultry products that are fully cooked and consumable without reheating. these products, including whole and sliced pork, beef, turkey, chicken, and variety of meats, in the forms of ham, roast, rolls, sausage, and frankfurter, are widely available in the delicatessen section of retail stores or various food service outlets. however, difficulties in avoidance of contamination by foodborne pathogens, notably listeria monocyto ...201524754253
international interlaboratory study comparing single organism 16s rrna gene sequencing data: beyond consensus sequence comparisons.this study presents the results from an interlaboratory sequencing study for which we developed a novel high-resolution method for comparing data from different sequencing platforms for a multi-copy, paralogous gene. the combination of pcr amplification and 16s ribosomal rna gene (16s rrna) sequencing has revolutionized bacteriology by enabling rapid identification, frequently without the need for culture. to assess variability between laboratories in sequencing 16s rrna, six laboratories sequen ...201527077030
molecular docking based virtual screening of compounds for inhibiting sortase a in l.monocytogenes.listeriosis is considered as an important public health issue. sortase a (srta) is an enzyme with catalytic role in l. monocytogenes that breaks the junction between threonine and glycine in the lpxtg motif (a key motif in internalin a (inla) that plays an important role in listeriosis). inactivation of srta was shown to inhibit anchoring of the invasion protein inia. this is in addition to inhibiting peptidoglycan-associated lpxtg proteins. therefore, it is of interest to inhibit stra using pot ...201526912950
controlling listeria monocytogenes scott a on surfaces of fully cooked turkey deli product using organic acid-containing marinades as postlethality dips.this study evaluated the efficacy of organic acids applied singly or in combination as postlethality dips to sliced uncured turkey deli loaves to inhibit the growth of listeria monocytogenes (lm) scott a. treatments consisted of sodium lactate (sl; 3.6%), potassium lactate (pl; 3.6%), sodium citrate (sc; 0.75%), a combination of sl and sodium diacetate (sda; 0.25%), and a combination of sl/pl/sda, alongside appropriate negative and positive controls. products were inoculated with 10(4)-10(5) cfu ...201526904656
prevalence of listeria monocytogenes in raw milk in kerman, iran.listeria monocytogenes as one of the most important pathogen in public health concerns is transmitted through consumption of contaminated food. the pathogen has been considered as a potential source of contamination of raw milk and dairy products. this research was aimed to investigate prevalence of l. monocytogenes in raw milk in kerman region. in the summer of 2011, a total number of one hundred raw milk samples were collected from bulk tanks of some dairy farms and tested for iap and acta gen ...201526893812
probabilistic models to predict listeria monocytogenes growth at low concentrations of nano2 and nacl in frankfurters.this study developed probabilistic models to describe listeria monocytogenes growth responses in meat products with low concentrations of nano2 and nacl. a five-strain mixture of l. monocytogenes was inoculated in nbye (nutrient broth plus 0.6% yeast extract) supplemented with nano2 (0-141 ppm) and nacl (0-1.75%). the inoculated samples were then stored under aerobic and anaerobic conditions at 4, 7, 10, 12, and 15℃ for up to 60 d. growth response data [growth (1) or no growth (0)] for each comb ...201526877642
growth potential of listeria monocytogenes and staphylococcus aureus on fresh-cut tropical fruits.the objective of this study was to evaluate the fate of staphylococcus aureus, listeria monocytogenes, and natural microbiota on fresh-cut tropical fruits (pitaya, mango, papaya and pineapple) with commercial pvc film at different storage temperature (5, 13, and 25 °c). the results showed that s. aureus, l. monocytogenes, and natural microbiota increased significantly on fresh-cut tropical fruits at 25 °c. both pathogen and natural microbiota were able to grow on fresh-cut tropical fruits at 13 ...201526862605
invasiveness of listeria monocytogenes strains isolated from animals in poland.animals are important reservoir of listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. however, data on invasiveness of l. monocytogenes strains of animal origin is very scarce. ability of 18 l. monocytogenes strains of animal origin to invade ht-29 cells was investigated. plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. almost 40% of l. monocytogenes strains were weakly invasive. it was shown tha ...201526812809
development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens.the objective of the present study was to formulate a multi-pathogen enrichment broth which could support the simultaneous growth of five common foodborne pathogens (salmonella enterica, staphylococcus aureus, shigella flexneri, listeria monocytogenes and escherichia coli o157:h7). the formulated broth sssle was composed of potassium tellurite, bile salt, lithium chloride, and sodium chloride as growth-inhibitors; glucose, esculin, mannitol and sodium pyruvate as growth-promoters. compared with ...201526782652
occurrence and antibiotic susceptibility of listeria species in turkey meats.the aims of this study were to investigate the occurrence of listeria species in turkey meats and to check the antimicrobial susceptibility of the isolated strains. hundred and fifteen raw turkey meat samples were randomly collected from the supermarkets, butchers and restaurants. strain isolation and identification were made according to the iso11290-1 method. antimicrobial susceptibility was determined by the standard disc diffusion method. a total of 47 listeria spp. were isolated from 115 (4 ...201526761896
survey of yogurt powder storage in ambient export countries a safety evaluation standard compliance and comparative analysis.yogurt powder is fermented milk processed in the form of dry yogurt, and has advantages such as stability, storability, convenience, and portability. china and vietnam are important export target countries because of the increased demand for dairy products. therefore, we surveyed dairy product standardization in order to establish an export strategy. lactic acid bacteria counts are unregulated in korea and vietnam. in china, lactic acid bacteria counts are regulated at 1×10(6) colony-forming uni ...201526761812
growth modelling of listeria monocytogenes in korean pork bulgogi stored at isothermal conditions.the purpose of this study was to develop predictive models for the growth of listeria monocytogenes in pork bulgogi at various storage temperatures. a two-strain mixture of l. monocytogenes (atcc 15313 and isolated from pork bulgogi) was inoculated on pork bulgogi at 3 log cfu/g. l. monocytogenes strains were enumerated using general plating method on listeria selective medium. the inoculated samples were stored at 5, 15, and 25℃ for primary models. primary models were developed using the barany ...201526761807
listeria monocytogenes infection in ruminants: is there a link to the environment, food and human health? a review.listeria (l.) monocytogenes is widely distributed in the environment, but also has the ability to cause serious invasive disease in ruminants and humans. this review provides an overview of listeriosis in ruminants and discusses our insufficient understanding of reservoirs and possible cycling ofl. monocytogenes between animal and human hosts, food and the environment. it indicates gaps in our knowledge of the role of genetic subtypes in l. monocytogenes ecology and virulence as well as risk fac ...201526753347
[quantitative detection on contamination of listeria monocytogenes isolated from retail ready-to-eat meats in beijing].to survey the contamination of l. monocytogenes isolated from retail ready-to-eat meats in beijing.201526738383
functional heterogeneity in cd4(+) t cell responses against a bacterial pathogen.to investigate how cd4(+) t cells function against a bacterial pathogen, we generated a listeria monocytogenes-specific cd4(+) t cell model. in this system, two tcrtg mouse lines, llo56 and llo118, recognize the same immunodominant epitope (llo190-205) of l. monocytogenes and have identical in vitro responses. however, in vivo llo56 and llo118 display vastly different responses during both primary and secondary infection. llo118 dominates in the primary response and in providing cd8 t cell help. ...201526697015
rapid detection of listeria by bacteriophage amplification and sers-lateral flow immunochromatography.a rapid listeria detection method was developed utilizing a511 bacteriophage amplification combined with surface-enhanced raman spectroscopy (sers) and lateral flow immunochromatography (lfi). anti-a511 antibodies were covalently linked to sers nanoparticles and printed onto nitrocellulose membranes. antibody-conjugated sers nanoparticles were used as quantifiable reporters. in the presence of a511, phage-sers nanoparticle complexes were arrested and concentrated as a visible test line, which wa ...201526694448
ongoing outbreak of invasive listeriosis, germany, 2012 to 2015.listeriosis patient isolates in germany have shown a new identical pulsed-field gel electrophoresis (pfge) pattern since 2012 (n = 66). almost all isolates (listeria monocytogenes serotype 1/2a) belonged to cases living in southern germany, indicating an outbreak with a so far unknown source. case numbers in 2015 are high (n = 28). no outbreak cases outside germany have been reported. next generation sequencing revealed the unique cluster type ct1248 and confirmed the outbreak. investigations in ...201526691727
complete genome sequences of two listeria monocytogenes serovars, 1/2a and 4b, isolated from dairy products in brazil.listeria monocytogenes is the foodborne pathogen responsible for a bacterial infection called listeriosis. here, we present the whole-genome sequences of two l. monocytogenes serovars, 1/2a and 4b, which are considered the most prevalent in food processing plants and listeriosis outbreaks, respectively.201526679596
[a clinical analysis of 16 patients with maternal listeriosis].to summarize the clinical characteristics and outcome of maternal listeriosis so as to improve the understanding of disease and the level of diagnosis and treatment.201526674793
rhombencephalitis due to listeria monocytogenes: a clinicopathologic study of a case.central nervous system infections caused by listeria monocytogenes usually manifest in the form of meningitis or meningoencephalitis, and are more common among immunosuppressed patients. brainstem encephalitis (rhombencephalitis) is less common and fatal if not recognized and treated early.201526671740
listeriolysin o, but not murine e-cadherin, is involved in invasion of listeria monocytogenes into murine liver parenchymal cells.human e-cadherin and listeriolysin o (llo) are involved in invasion of listeria monocytogenes into human liver parenchymal cells (lpc). yet, it remains to be determined whether murine e-cadherin and llo participate in invasion of l. monocytogenes into murine lpc. in the present study, involvement of murine e-cadherin and llo in invasion of l. monocytogenes into murine lpc was investigated. murine e-cadherin was expressed on murine lpc, but the expression became undetectable by insertion of trans ...201526668665
plasmonic enzyme-linked immunosorbent assay using nanospherical brushes as a catalase container for colorimetric detection of ultralow concentrations of listeria monocytogenes.plasmonic enzyme-linked immunosorbent assay (pelisa) based on catalase (cat)-mediated gold nanoparticle growth exhibits ultrahigh sensitivity for detecting disease-related biomarkers using sandwich formats. however, the limit of detection (lod) of this strategy for listeria monocytogenes is only around 10(3) cfu/ml, which considerably exceeds the amount of l. monocytogenes commonly present in food products (<100 cfu/g). herein, we report an improved pelisa method for detection of l. monocytogene ...201526646325
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