Publications

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[molecular-epidemiological characteristic and possible origin of vibrio cholerae non o1/non o139 with complete and limited set of virulence genes].study of molecular-epidemiological characteristics of vibrio cholerae non o1/non o139 serogroup with complete and limited set of virulence genes was performed. differences of their genes composition as compared to these of o1 serogroup (classic and el tor biovars) were revealed, which points to their origin from avirulent environmental cholera vibrios.200718038543
environment and virulence factors of vibrio cholerae strains isolated in argentina.to determine the presence of vibrio cholerae in different areas of argentina in three sample types, to determine the composition of planktonic communities in areas at which this pathogen was detected and to characterize the virulence properties and antimicrobial resistance of the recovered environmental isolates.200718045430
construction and preclinical evaluation of recombinant peru-15 expressing high levels of the cholera toxin b subunit as a vaccine against enterotoxigenic escherichia coli.enterotoxigenic escherichia coli (etec) is the leading cause of traveler's diarrhea. the heat-labile (lt) and heat-stable (st) toxins mediate etec induced diarrhea. etec strains may express lt, st, or both lt and st, with lt-expressing strains accounting for approximately 50-60% of etec-related traveler's diarrhea. cholera toxin (ct) is >80% homologous to lt and vaccination with ct-b subunit (ct-b) -based vaccines elicit a protective immune response against lt-producing etec strains. peru-15 is ...200718045752
evaluation of in vivo and in vitro biological activity of a vibrio cholerae 01 hemolysin.to evaluate the hemolysin effect by ileal loop model produced by vibrio cholerae o1 strains, compared with the cellular lysis or cytotoxic activity (ca) observed in cell culture.200718053392
microbiology: bilingual bacteria. 200718063996
[biofilm formation and response to oxidative stress in pseudomonas aeruginosa and vibrio cholerae non-o1 depending on culture media].to evaluate the effect of six culture media (five complex and one mineral) on biofilm formation and response to oxidative stress in pseudomonas aeruginosa (3 strains) and vibrio cholerae non-o1 (3 strains).200718064800
clinical characteristics of non-o1/non-o139 vibrio cholerae isolates and polymerase chain reaction analysis of their virulence factors.non-o1/non-o139 vibrio cholerae can cause invasive extraintestinal disease as well as enteritis. the pathogenesis of invasive non-o1/non-o139 v. cholerae infections remains to be determined. this study compared the clinical manifestations and predisposing factors between bacteremic and non-bacteremic non-o1/non-o139 v. cholerae infections and examined virulence-associated genes in the pathogenic strains causing invasive disease.200718087626
a new in vitro strand transfer assay for monitoring bacterial class 1 integron recombinase inti1 activity.inti1 integrase is a tyrosine recombinase involved in the mobility of antibiotic resistance gene cassettes within bacterial class 1 integrons. recent data have shown that its recombination specifically involves the bottom strand of the attc site, but the exact mechanism of the reaction is still unclear. an efficient in vitro assay is still required to better characterize the biochemical properties of the enzyme. in this report we describe for the first time an in vitro system partially reproduci ...200718091989
soj (para) dna binding is mediated by conserved arginines and is essential for plasmid segregation.soj is a member of the para family of atpases involved in plasmid and chromosomal segregation. it binds nonspecifically and cooperatively to dna although the function of this binding is unknown. here, we show that mutation of conserved arginine residues that map to the surface of bacillus subtilis soj caused only minimal effects on nucleotide-dependent dimerization but had dramatic effects on dna binding. using a model plasmid partitioning system in escherichia coli, we find that soj dna-binding ...200718077387
[development and application of real-time polymerase chain reaction to detect vibrio cholerae o1 and o139 in river water].to develop a real-time sybr green polymerase chain reaction (pcr) for detection of vibrio cholerae serogroups o1 and o139, and to evaluate its reliability through detection of estuary water samples.200718080563
[an avirulent vibrio cholerae strain--producer of the cholera toxin b subunit: obtaining and molecular genetic analysis].the conjugative recombinant plasmid piem3 (kmr tcr) was constructed in order to introduce the cloned ctxb gene encoding the cholera toxin b subunit into the vibrio cholerae cells. the plasmid was obtained as a result of co-integration of two plasmids: a conjugative plasmid, piem1(kmr), carrying mini-kan transposon and is1 element, as well as the pctdelta27(tcr) plasmid that is a derivative of the pbr322 which carries the cloned ctxb gene. the avirulent vibrio cholerae strain eltor biovar deprive ...200718154075
detection and identification of enterohemorrhagic escherichia coli o157:h7 and vibrio cholerae o139 using oligonucleotide microarray.the rapid and accurate detection and identification of the new subtype of the pathogens is crucial for diagnosis, treatment and control of the contagious disease outbreak. here, in this study, an approach to detect and identify escherichia coli o157:h7 and vibrio cholerae o139 was established using oligonucleotide microarray. we coupled multiplex pcr with oligonucleotide microarray to construct an assay suitable for simultaneous identification of two subtypes of the pathogens.200718154687
negative matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry fragmentation of synthetic analogs of the o-specific polysaccharide of vibrio cholerae o:1 in the presence of anionic dopants.oligosaccharides (tri- to hexamers) that represent terminal epitopes of o-antigens of vibrio cholerae o:1, serotypes ogawa and inaba, have been studied by negative matrix-assisted laser desorption/ionization time of flight/time-of-flight mass spectrometry (maldi tof/tof ms). the [m - h(+)](-) ions are formed after expulsion of a proton from molecules studied under condition of maldi ms analysis in the negative mode. several ammonium salts (chloride, nitrate, hydrogencarbonate and hydrogensulfate ...200718192728
[the development of peroxosolvate-based composites and the evaluation of their effectiveness against extremely dangerous infective pathogens].the article contains the results of the test of pfk-m composition bactericidal activity against a range of extremely dangerous infective pathogens. the preparation proved to be highly effective for the disinfection of surfaces, sanitary and technical equipment, linen, dishes, and medical utensils contaminated by plague, cholera, and anthrax pathogens. the study found that the antimicrobial activity of the preparation increased after heating and lowered when the object to be disinfected was conta ...200718225504
[horizontal transfer of bacterial genes and its significance for antibiotic resistance and pathogenicity].* sexual reproduction does not occur in bacteria. the point of departure in bacterial reproduction is always that one individual divides itself into two identical descendants. * in the bacterial world, however, there is certainly exchange of hereditary characteristics (dna). this type of exchange is called horizontal gene transfer. * there are 3 basic ways for the exchange of dna between bacteria: conjugation, transduction and natural transformation. each of these has its specific impact on the ...200718225789
vibrio cholerae in the environment: a simple method for reliable identification of the species.a simple screening and identification protocol was assessed for the efficient distinction of colonies of vibrio cholerae species from others obtained on thiosulphate citrate bile salts sucrose agar after isolation from different environmental specimens. it was demonstrated here that the yellow colonies (sucrose-fermenting), which are able to grow on nutrient agar without added nacl and which present a positive oxidase reaction, can be confidently considered as presumptive v. cholerae. confirmati ...200718330064
modulation of vibrio cholerae porin function by acidic ph.the outer membrane of gram-negative bacteria contains porins, large pore-forming proteins which allow the traffic of hydrophilic compounds between the external medium and the periplasm. the oral mode of infection of vibrio cholerae, the agent of cholera, implies that the bacteria must adapt to severe changes in the environment, such as acidic ph and the presence of bile. because of their localization and the regulation of their expression in response to these external factors, the ompu and ompt ...200718690017
contamination of community water sources by potentially pathogenic vibrios following sea water inundation.potentially pathogenic members of the vibrionaceae family including vibrio cholerae and vibrio parahemolyticus were isolated from domestic sources of drinking water in coastal villages following sea water inundation during the tsunami in southern india. phenotypic and genotypic studies were done to confirm the identity and detection of toxins. vibrio-gyr (gyrase b gene) was detected in all sixteen vibrio isolates. toxin regulating genes i.e.: ctx gene, tdh gene, and trh gene, however were not de ...200718697592
outbreak of cholera in a labour encampment in suburbs of a modern city in north india. 200718697595
growing problem of multidrug-resistant enteric pathogens in africa.control of fecal-orally transmitted pathogens is inadequate in many developing countries, in particular, in sub-saharan africa. acquired resistance to antimicrobial drugs is becoming more prevalent among vibrio cholerae, salmonella enteritidis, diarrheagenic escherichia coli, and other pathogens in this region. the poor, who experience most of the infections caused by these organisms, bear the brunt of extended illness and exacerbated proportion of deaths brought about by resistance. improved an ...200718217545
[study on the application of pulsed-field gel electrophoresis regarding infection sources identification during an outbreak of vibrio cholerae in jiangxi province].to study the correlation between vibrio cholerae strains isolated from natural enviroment and fishery products and the source of infection during v. cholerae outbreaks.200718251274
[specificity of lectin receptors of cholera vibrios].spectrum of carbohydrate specificity of lectin receptors of epidemically significant cholera vibrios (ctx(+) tcp(+) hly(-)) as well as non epidemic hemolytic variants with or without tcp a gene (ctx(-) tcp(-) hly(+), ctx(-) tcp(+) hly(+)) was studied under the carbohydrates-mediated inhibition of hemagglutination between human erythrocytes of four blood groups and sheep erythrocytes. it was demonstrated that in toxigenic cultures lectin receptors specific for glucose, mannose, sacharose, lactose ...200718277542
[prophage ctxphi genome variability and its role in alteration of vibrio cholerae el tor virulence characteristics].comparative analysis of ctxphi prophage genome of 366 v. cholerae el tor strains isolated from infected people and water was carried out using the polymerase chain reaction. four groups of vibrios, which carry different combinations of ctxa, zot, and ace genes from core region of ctxphi prophage coding key (cholera enterotoxin) and accessory (zot and ace toxins) pathogenicity factors, were determined: ctxa(+) zot(-) ace(+), ctxa(-) zot(+) ace(+), ctxa(-) zot(+) ace(-), ctxa(-) zot(-) ace(+). vib ...200718283731
[the importance of circulating vibriocidal antibodies in a pos-epidemic region of diarrhea, são bento do una, state of pernambuco].the levels of vibriocidal antibodies were investigated among 41 adults without any past or present history of diarrhea due to vibrio cholerae o1 who were living in the municipality of são bento do una, pernambuco. a diarrhea outbreak occurred in this locality at the beginning of 2004, involving multiple bacterial agents, including vibrio cholerae. the microtitration test was used to investigate the presence of anti-ogawa and anti-inaba vibriocidal serum antibodies. vibriocidal titers e" 1:640 we ...200718200426
[advances in study of novel absorption enhancers based on tight junctions].hydrophilic low molecular drugs, peptides and proteins, which are always poor in bioavailability, are mainly absorbed through the paracellular way in which the tight junction is the elementary framework. the tight junctions are a multiple unit structure composed of multiprotein complex that affiliates with the underlying apical actomyosin ring. tight junction proteins are identified including transmembrane proteins (occludin, claudin and jam) , cytoplasmic plaque proteins (zo-1, zo-2, zo-3 and c ...200718300465
emergence of vibrio cholerae oi biotype eit or serotype inaba in aligarh. 200718306613
prevalence of acute diarrhoea in kathmandu valley.this retrospective study was conducted during january to september in the year 1997. three hundred and forty nine stool samples were collected from diarrhoea patients from different places of kathmandu valley and examined at national public health laboratory (nphl), teku, kathmandu. acute diarrhoea becomes epidemic in rainy season and is a major public health problem of the city. in this study, people with poor hygiene practice and poor education were infected more than other people. among the 3 ...200718340369
vibrio cholerae: model organism to study bacterial pathogenesis--interview. 200718979011
backbone and side chain 1h, 15n and 13c assignments for the reduced form of the oxidoreductase protein dsba from vibrio cholerae.we have determined 13c/15n/1h assignments for the reduced and oxidised forms of vibrio cholerae dsba (vcdsba). these form the basis for ongoing studies aimed at characterising the dynamics observed in the different redox forms of this bacterial oxidoreductase enzyme.200719636831
influence of environmental factors on the presence of vibrio cholerae in the marine environment: a climate link.evidence indicates that the atmospheric and oceanic processes that occur in response to increased greenhouse gases in the broad-scale climate system may already be changing the ecology of infectious diseases. recent studies have shown that climate also influences the abundance and ecology of pathogens, and the links between pathogens and changing ocean conditions, including human diseases such as cholera. vibrio cholerae is well recognized as being responsible for significant mortality and econo ...200719734600
[derivative spectroscopy and its use in sanitary hygiene control].an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...20072099444
architectonics of the parietal and temporal association cortex in the strepsirhine primate galago compared to the anthropoid primate macaca.a number of higher order association areas have been described in the parietal and temporal cortex of large-brained anthropoid primates such as macaca. however, little is known about the evolution of these areas, and the existence of homologous areas has not yet been clearly demonstrated in other mammalian groups. we addressed this issue by comparing the myelo- and cytoarchitecture of posterior association cortex in the anthropoid macaca to that of the small-brained, strepsirhine ("prosimian") p ...20071939733
a comparative study for fiberoptic and video endoscopic determination of the extent in minimal changes of gastric mucosa using indigo dye spraying.the image resolution of fiberoptic and video endoscopy was compared in a series of patients with early gastric cancer. eighteen patients were divided into two groups. one group of patients (n = 9) was evaluated with fiberoptic endoscopy while the second group (n = 9) was evaluated using video endoscopy. the extent of cancer invasion, with special attention to size, shape and type of the lesions, was evaluated in each patient. attempts were also made to identify the proximal and distal limits of ...20072374986
[anti-endotoxin monoclonal antibodies in the treatment of gram-negative germ sepsis]. 20071853112
regulation of alcohol consumption by the renin-angiotensin system: a review of recent findings and a possible mechanism of action.the renin-angiotensin system has traditionally been associated with the regulation of fluid and electrolyte balance. in this review we summarize the data which ascribes a completely new function to this system, i.e., the regulation of alcohol consumption. in addition, we suggest a possible mechanism for this effect based on the concept of a satiety or stop process. the approach taken was to examine the effect on alcohol intake of a wide variety of drug, genetic, dietary, surgical and neurosurgic ...20071852316
hla-dr gene frequencies in the japanese population obtained by oligonucleotide genotyping.we determined hla-drb types of 375 randomly chosen healthy japanese donors using a set of 29 different sequence-specific oligonucleotide (sso) probes directed against various drb alleles. except for a few cases, these ssos enabled us to identify 33 different drb types including those detectable only by sso genotyping. gene frequencies were calculated for each of the drb types identified. the "blank" frequency calculated by our sso typing was essentially zero, in contrast to the considerably high ...20071839093
discordant expression of tuberous sclerosis in monozygotic twins.a set of 20-year-old female japanese twins, most probably monozygotic, had clinical evidence of tuberous sclerosis (ts). they were discordant for symptoms. one of the twins exhibited facial red-brown papules (adenoma sebaceum), a dorsal shagreen patch, intracerebral calcifications, angiomyolipoma in the right kidney, and hypopigmented macules; the other had only a few hypopigmented macules. modification of ts gene expression by the effects of environmental (extrinsic) factors is suggested.20071885853
hypopituitarism following snake bite.a patient who developed hypopituitarism after viperine envenomation is described. thrombosis as a part of disseminated intravascular coagulation may have been the cause. hypopituitarism should be suspected in such cases especially when there is associated acute renal failure.20071800512
teaching problem solving in pathology. general professional education of physicians (gpep) anticipated.the association of american medical colleges' 1984 publication, "physicians for the 21st century"--commonly known as the gpep (general professional education of physicians) report--advocates curricula that foster active, independent learning and acquisition of problem-solving skills. a curriculum along these principles was previously designed by the pathology department of the university of california school of medicine, davis. our experience at davis has shown that the curricular goals of the g ...20073755887
[age-sex aspects of distribution of children with scleroderma according to clinical forms]. 20072075074
genetic markers in the blood of multiple sclerosis patients.poligenetically determined predisposition to multiple sclerosis (ms) defines the way of immunological reaction to environmental factors and leads to clinically manifest disease. although the connection between ms and some loci of the hla system has been established, the hereditary predisposition to ms remains to be elucidated. we determined the phenotypes of monogenic hereditary characteristics linked to the surface of red blood cells that were obtained from 45 ms patients and 458 healthy subjec ...20071810395
glutathione disulfide formation and oxidant stress during acetaminophen-induced hepatotoxicity in mice in vivo: the protective effect of allopurinol.acetaminophen (500 mg/kg i.p.) induced hepatotoxicity in fasted icr mice in vivo. acetaminophen also caused a long-lasting 50% reduction of the hepatic atp content, an irreversible loss of hepatic xanthine dehydrogenase activity and a transient increase of the xanthine oxidase activity. all effects occurred before parenchymal cell damage, i.e., the release of cellular enzymes. the hepatic content of gsh and gssg was initially depleted by acetaminophen without affecting the gssg:gsh ratio (1:200) ...20072262912
effects of dialysis membranes on beta 2-microglobulin production and cellular expression.we investigated the effects of different dialysis membranes on the production of beta 2-microglobulin (beta 2m) in peripheral blood mononuclear cell cultures (pbmnc) obtained from hemodialysis patients in a prospective cross-over design study. chronic dialysis with cuprophane membrane leads to an increase in beta 2m production from 129 +/- 11 ng/ml to 192 +/- 23 ng/ml (p less than 0.002). this increase is reversed by the use of a non-complement activating membrane polymethylmethacrylate. in addi ...20072266681
effects of dialysis membranes on beta 2-microglobulin production and cellular expression.we investigated the effects of different dialysis membranes on the production of beta 2-microglobulin (beta 2m) in peripheral blood mononuclear cell cultures (pbmnc) obtained from hemodialysis patients in a prospective cross-over design study. chronic dialysis with cuprophane membrane leads to an increase in beta 2m production from 129 +/- 11 ng/ml to 192 +/- 23 ng/ml (p less than 0.002). this increase is reversed by the use of a non-complement activating membrane polymethylmethacrylate. in addi ...20072266681
cesarean section for the birth of the second twin. 20072223696
cesarean section for the birth of the second twin. 20072223696
exercise performance and limiting factors in patients with chronic lung diseases.to demonstrate the clinical usefulness of the treadmill exercise test in patients with chronic lung diseases (cld), 272 consecutive tests done from jan. 1986 to dec. 1988 were analyzed. the subjects mainly consisted of patients with chronic obstructive pulmonary disease (copd) and interstitial lung disease (ild). with the present incremental treadmill protocol the maximal stage attained by a patient or distance walked were closely related to the patient's maximal oxygen uptake. comparing symptom ...20072074970
clearance of ceftriaxone in critical care patients with acute renal failure.serum concentrations of ceftriaxone (rocephintm), a third generation cephalosporin, were monitored in 5 operative intensive care patients suffering from acute renal failure (arf) and compared to those of 7 patients without renal disturbance. for a period of 7 days, a fixed dose of 2 g/day was given by a 15 min infusion. pharmacokinetic parameters were calculated by fitting all serum and urine data measured over the period of treatment. ceftriaxone free fraction was measured on days 2 and 7. ther ...20072269714
[cognition, memory and affect].the flow of information within the brain and the sequence of operations which process it get enriched through a twofold mediation, that of the current affective states and that of the references made to an individual life-history. affectivity and memory are closely linked in the elaboration of action (thought acts, speech acts, behaviours) since it is especially through the mediation of affective states and emotions that the current action is both a partial reactualization of the past and one of ...20072096709
tentorial traversal by ependymoblastoma. 20071899507
constrictive pericarditis versus restrictive cardiomyopathy: a reappraisal and update of diagnostic criteria.distinguishing constrictive pericarditis from restrictive cardiomyopathy is a difficult clinical challenge. we review published reports in which hemodynamic criteria were used to differentiate these two diagnoses. there were 82 cases of constriction and 37 cases of restriction. the overall predictive accuracy of the difference between right and left ventricular end-diastolic pressures (rvedp and lvedp), rv systolic pressure, and the ratio of rvedp to rv systolic pressure were 85%, 70%, and 76%, ...20071951008
cholestatic jaundice associated with captopril therapy.captopril has attained widespread use as an effective agent in the treatment of heart failure and hypertension. dermatological, renal and haematological toxicity associated with its use has been widely described and is usually well recognized. there have been comparatively few reports implicating it as causing hepatic drug reactions. most descriptions have emphasized strongly cholestatic features, although a mixed hepatocellular cholestatic picture and predominant hepatocellular reactions have b ...20071932674
[impact of the development of a rural health center on the demand for emergency care].the new legislation on primary health care (phc) has reorganized the implementation of services. we have evaluated the impact on the demand of emergency attention in the rural town of almodóvar del pinar (cuenca); this attention was previously given by the apd physician and now partially depends from the health center. overall 97 cases were assisted, as compared with 229 in the preceding period (p less than 0.001). the motives for consultation remained unchanged, as well as the timing of the dem ...20072518912
familial predisposition for herniation of a lumbar disc in patients who are less than twenty-one years old.the parents of sixty-three patients who were less than twenty-one years old and who had operatively confirmed herniation of a lumbar disc were interviewed regarding a history of sever back pain, sciatica, and herniated disc, to determine whether aggregation of herniation of a lumbar disc occurs in families of patients in this young age-group. the parents of sixty-three additional patients who had a non-spinal orthopaedic diagnosis (control group) were matched for age and sex with the study group ...20071824705
alternating-pattern z-plasties in association with v-y advancements: a new idea of multiple z-plasties.a new principle of arrangement in multiple z-plasties is advocated. by placing z's in an alternating pattern in combination with v-y plasties, more effective and harmless elongation of skin will be possible.20071852835
counting the cost. 20071865688
ph measurements with an ion sensitive field effect transistor in the mouth of patients with xerostomia.a transistor ph electrode (ion sensitive field effect transistor), placed in the upper dentures of eleven xerostomia patients and five healthy volunteers, was used to register ph changes in five-, six- and seven-day-old dental plaque. a mouth rinse with a 10% sucrose solution caused a ph fall of about three decades. a significant difference in duration of critical plaque ph was observed: in xerostomia patients, a 10% longer period of ph less than 5.7 was registered during 60 min following a sucr ...20071855797
family caregiving: personal and social costs. 20071794398
diagnosis of ectopic pregnancy using transvaginal ultrasound scanning.a new technique was developed for transvaginal ultrasound scanning in the routine evaluation of women with suspected early ectopic pregnancy. this approach enables us to detect early pregnancies that could not be visualized previously with routine abdominal ultrasound. transvaginal scanning shows the location of the gestational sac very clearly, whether it is within or outside the uterus. our introduction of this technique into the evaluation process has reduced patients' risk by minimizing dela ...20072649669
axonal pathfinding during the regeneration of the goldfish optic pathway.retinal ganglion cells in fish and amphibians regenerate their axons after transection of the optic nerve. fiber tracing studies during the third month of regeneration show that the axons have reestablished a basically normal fiber order in the two brachia of the optic tract; axons originating in the ventral hemiretina are concentrated in the dorsal brachium, axons from the dorsal hemiretina in the ventral brachium. attardi and sperry (exp. neurol. 7:46-64, 1963) first suggested that the reestab ...20072754027
[brain stem auditory evoked potentials in the early period after a brain concussion].acoustic truncal evoked potentials (atep) were studied in 24 patients in the first 48 hours after concussion of the brain. the absolute peak latency of components 1, 3, and 5, the interpeak intervals 1-3, 3-5, and 1-5, and the interaural difference were determined. the results were compared with those of examination of a control group of 24 healthy individuals. individual and group appraisal failed to reveal any significant differences from normal values in none of the studied parameters in pati ...20072168645
failure of chronic glutathione elevation to reduce cytotoxicity produced by exposure to cis-diamminedichloroplatinum(ii), ionizing radiation, or hyperthermia.chinese hamster ovary (cho) cells cultured in vitro were continuously exposed to increasing concentrations of diethylmaleate (dem). chronic exposure of these cells (designated cho/dem) to 80 microm diethylmaleate resulted in an increase in cystine transport, a decrease in glutathione inhibition of the enzyme gamma-glutamylcysteine synthetase, elevation of intracellular glutathione levels to 4.3 times control, and elevation of glutathione-s-transferase activity by 6.6 times. yet, cho/dem and cont ...20071974822
adverse events temporally associated with immunizing agents: 1987 report. 20072804852
results of cochlear implants in children.about 700 children throughout the world have been implanted to date. placing a cochlear implant represents no particular problem in the child. the incidence of complications is not high, being of about the same proportion as that in the adult. to obtain the best results, the selection of patients must be correct and carried out by a multi-disciplinary team of specialists, while the operated child must be subject to regular and intensive rehabilitation. the results appear to have become more sati ...20071950547
validation of biologic markers for use in research on chronic fatigue syndrome.unresolved aspects of chronic fatigue syndrome can be addressed by research involving biologic markers. these may be any molecular, biochemical, physiological, or other biologic parameter obtainable from biologic specimens. the use of biologic markers in research requires their validation as dependent or independent variables. additionally, other characteristics of markers such as reliability of assays, background level, confounding factors, interpretations, and legal and ethical implications sh ...20072020808
seasonal variation in the proliferation fraction of australian common nevi.image cytometry was used to assess seasonal variation in the proliferation fraction of australian common nevi. twenty pairs of nevi were evaluated. one member of each pair had been excised during the australian summer, and the other member of the pair had been excised during the winter. the nevi were matched for age, sex, and body site. from each nevus, 6-microns sections were feulgen-stained and evaluated with a cas 200 image analyzer, which was used to obtain dna histograms. proliferation frac ...20071951983
total body potassium, skeletal muscle potassium and magnesium in patients with bartter's syndrome. 20071796974
total body potassium, skeletal muscle potassium and magnesium in patients with bartter's syndrome. 20071796974
[preprosthetic orthodontics]. 20072630496
[preprosthetic orthodontics]. 20072630496
[urinary symptoms in elderly women in nursing homes. frequency and social consequences of urinary incontinence in elderly women living in nursing homes].all 278 female residents of nursing homes in the county of roskilde were assessed as regards the extent and severity of urinary incontinence and possible urinary infections. a total of 168 (58.5%) were incontinent. culture of the urine was performed in 210 women and significant bacteriuria was demonstrated in 50%. thirty-four out of the total of 287 women (12%) had indwelling catheters. in incontinent women without catheters, great quantities of urine were often voided involuntarily. despite thi ...20072238207
[urinary symptoms in elderly women in nursing homes. frequency and social consequences of urinary incontinence in elderly women living in nursing homes].all 278 female residents of nursing homes in the county of roskilde were assessed as regards the extent and severity of urinary incontinence and possible urinary infections. a total of 168 (58.5%) were incontinent. culture of the urine was performed in 210 women and significant bacteriuria was demonstrated in 50%. thirty-four out of the total of 287 women (12%) had indwelling catheters. in incontinent women without catheters, great quantities of urine were often voided involuntarily. despite thi ...20072238207
preventing the kidney disease of diabetes mellitus: public health perspectives. 20072643304
lymph node staging in patients with clinically negative neck examinations by ultrasound and ultrasound-guided aspiration cytology.to assess the usefulness of ultrasound and ultrasound-guided fine-needle aspiration cytology in detecting occult cervical lymph node metastases, 107 patients with squamous cell carcinoma of the head and neck, who underwent 132 elective neck dissections, had preoperative ultrasound examination. during the assessment of the last 54 patients, who underwent 70 elective neck dissections, ultrasound-guided aspiration cytology was available. although ultrasound was able to detect lymph node metastases ...20071951890
partial laryngectomy for glottic cancer after high-dose radiotherapy.a vertical partial laryngectomy (vpl) for salvage was performed on 25 patients with locally persistent or recurrent squamous cell carcinoma of the vocal cord(s) after high-dose radiotherapy at the ucla medical center between 1969 and 1988. patients were followed for a minimum of 2 years and a median of 4.4 years after vpl. ninety-six percent of patients remained free of disease. tumor was controlled in patients with impaired vocal cord mobility and involvement of the contralateral cord or false ...20071951885
[the development of osseointegrated implants]. 20071932767
testicular cancer, dimethylformamide, and leather tanneries. 20071978094
[bacterial sepsis and nosocomial infection: a study of 70 patients].74 episodes of bacterial sepsis in 70 patients were analysed from july 1988 to june 1989. the incidence of sepsis is 0.7% of the whole admitted patients. compared to a similar previous study from april 1982 to march 1983, the incidence of sepsis decreased (p less than 0.01), and the incidence of nosocomial sepsis remained unchanged, whereas its mortality decreased (p less than 0.05). the host defenses are important in predisposing to sepsis and determining prognosis. antibiotic sensitivity test ...20071794243
[advantages and limits of programmed autologous transfusion in cardiovascular surgery. apropos of 524 patients].between october 1987 and july 1989, 544 patients, candidates for cardiovascular surgery, were included in a trial of programmed autologous autotransfusion. five hundred and twenty four patients underwent one or several (maximum 4) blood donation sessions in the 3 weeks before surgery with no complications. overall, 57% of patients benefited from homologous blood transfusion, thereby avoiding all risk of contamination. it was in the group of patients able to undergo 3 or 4 preoperative blood dona ...20071759897
[osmotic homeostasis and blood rheology in patients in the acute period of ischemic cerebral infarction].the authors describe the role of changes in osmotic homeostasis and hemorheology in the pathogenesis of ischemic brain stroke. 48 patients experiencing an acute period of ischemic brain stroke were examined. for this purpose the following methods were employed: measurements of blood, urine and csf osmolality by knauer osmometer; glucose and urea by the enzymatic assay; blood electrolytes by flame photometer; aggregation of platelets, red blood cells, blood viscosity, fibrinogen, hematocrit; the ...20072175076
plasma amine oxidase activities in norrie disease patients with an x-chromosomal deletion affecting monoamine oxidase.two individuals with an x-chromosomal deletion were recently found to lack the genes encoding monoamine oxidase type a (mao-a) and mao-b. this abnormality was associated with almost total (90%) reductions in the oxidatively deaminated urinary metabolites of the mao-a substrate, norepinephrine, and with marked (100-fold) increases in an mao-b substrate, phenylethylamine, confirming systemic functional consequences of the genetic enzyme deficiency. however, urinary concentrations of the deaminated ...20072018626
strikingly low atpase activities in flagellar axonemes of a chlamydomonas mutant missing outer dynein arms.the atpase activities in chlamydomonas axonemes were compared between wild type and a mutant (oda) that lacks entire outer dynein arms, at various ionic strengths and ph values, and in the presence of different concentrations of high-molecular-mass dextran. over a 0-0.2 m kcl concentration range, the atpase activity of oda axonemes was found to be 5-12 times lower than that of the wild-type axonemes. the low activity in oda is surprising since outer arm-depleted axonemes of sea urchin sperm have ...20072140096
strikingly low atpase activities in flagellar axonemes of a chlamydomonas mutant missing outer dynein arms.the atpase activities in chlamydomonas axonemes were compared between wild type and a mutant (oda) that lacks entire outer dynein arms, at various ionic strengths and ph values, and in the presence of different concentrations of high-molecular-mass dextran. over a 0-0.2 m kcl concentration range, the atpase activity of oda axonemes was found to be 5-12 times lower than that of the wild-type axonemes. the low activity in oda is surprising since outer arm-depleted axonemes of sea urchin sperm have ...20072140096
antinuclear antibody-negative systemic lupus erythematosus (sle) and severe renal involvement: close correlation between disease activity and appearance of circulating anticoagulant.a 16-year-old girl meeting the criteria for sle is described. salient features of the clinical course included active glomerulonephritis with dense subepithelial deposits on electron microscopy, pulmonary embolism, axillary vein thrombosis, arthritis, serositis and fever. disease activity correlated with the presence of lupus anticoagulant as measured by vdrl and partial thromboplastin time (ptt). her serum was consistently negative to ana, anti-ds-dna, anti-ss-dna, ena, anti-ro, anti-la, and le ...20073128493
phylogenetic approach to endocrine-immune system interactions.the survival of organisms in a hostile and highly variable environment is dependent upon the proper balance of physiological processes. according to emerging views, homeostasis may be achieved by the coordinated activities of the three major integrative systems: the nervous, endocrine, and immune systems. although each of these systems is replete with its particular molecules, cells, tissues, and organs, often not seemingly integrated into the whole organism, homeostasis is uncompromised and the ...20071974803
mechanistic aspects of cytochrome p-450-hydroperoxide interactions: substituent effects on degradative pathways. 20072680387
hybridocytochemistry with non-radioactive probes as a tool for biomedical research and cytodiagnostics.the advent of dna and rna labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. there are already practical applications. however, in order to fully compete with radioactive procedures an increase in sensitivity is still desired.20072435580
vecuronium infusion requirements in pediatric patients during fentanyl-n2o-o2 anesthesia.eighty-one pediatric patients, ranging from neonates to adolescents, were studied during fentanyl-n2o-o2 anesthesia to determine for each of them the vecuronium infusion required to maintain 90-95% neuromuscular block (nmb). electromyographic monitoring with train-of-four stimuli was used. the steady infusion rate was 62 +/- 15 (sd) micrograms.kg-1.hr-1 in neonates and infants. this rate was 40% of that required by children 3 to 10 years old (154 +/- 49 micrograms.kg-1.hr-1; p less than 0.05). i ...20072562909
remembrances of the meadowbrook conferences--when steroids came to dna. 20071855458
[anti-dna antibody, anti-idiotypic antibody and systemic lupus erythematosus]. 20072258660
[anti-dna antibody, anti-idiotypic antibody and systemic lupus erythematosus]. 20072258660
[anticancer effect of high voltage pulses combined with concentration dependent anticancer drugs on lewis lung carcinoma, in vivo].using high voltage pulse (initial electric field strength 3kv/cm, duration 4 msec, applied energy 14.9 joule) as a local load, and concentration dependent anticancer drugs such as peplomycin (pep) 20 mg/kg, cyclophosphamide (cpa) 100 mg/kg, mitomycin c (mmc) 3 mg/kg and cisplatin (cddp) 5 mg/kg administrated intraperitoneally, the effects on the suppression of primary lesions and on formation of pulmonary metastasis in the spontaneous pulmonary metastatic system of lewis lung carcinoma were inve ...20072277215
[anticancer effect of high voltage pulses combined with concentration dependent anticancer drugs on lewis lung carcinoma, in vivo].using high voltage pulse (initial electric field strength 3kv/cm, duration 4 msec, applied energy 14.9 joule) as a local load, and concentration dependent anticancer drugs such as peplomycin (pep) 20 mg/kg, cyclophosphamide (cpa) 100 mg/kg, mitomycin c (mmc) 3 mg/kg and cisplatin (cddp) 5 mg/kg administrated intraperitoneally, the effects on the suppression of primary lesions and on formation of pulmonary metastasis in the spontaneous pulmonary metastatic system of lewis lung carcinoma were inve ...20072277215
[hemoglobin, serum iron and transferrin saturation among users of intrauterine devices and oral contraceptive agents].we studied 60 females using either intrauterine device or taking oral contraceptive pills. hemoglobin, serum iron, total iron binding capacity and saturation of transferrin were determined before and 4 and 10 months after starting a responsible paternity program. women with a basal hemoglobin level below 12 g/dl were excluded. age, parity and hematologic parameters were similar for both groups. a significant decrease in hemoglobin level and saturation of transferrin was observed at 10 months in ...20072293270
[hemoglobin, serum iron and transferrin saturation among users of intrauterine devices and oral contraceptive agents].we studied 60 females using either intrauterine device or taking oral contraceptive pills. hemoglobin, serum iron, total iron binding capacity and saturation of transferrin were determined before and 4 and 10 months after starting a responsible paternity program. women with a basal hemoglobin level below 12 g/dl were excluded. age, parity and hematologic parameters were similar for both groups. a significant decrease in hemoglobin level and saturation of transferrin was observed at 10 months in ...20072293270
neosalpingostomy for distal tubal obstruction: prognostic factors and impact of surgical technique.we reviewed the clinical records of all women who underwent microsurgical terminal neosalpingostomy for distal tubal obstruction between january 1983 and june 1988. we identified 95 women whose preoperative evaluation revealed no other contributory factors for infertility and analyzed their pregnancy outcome after this procedure. pregnancy success was inversely related to the extent of tubal distortion (dilation, rugal integrity, and status of the fimbria) and degree of adnexal adhesions. using ...20072245857
imaging of chronic renal infections. 20072117344
studies of experimental hosiery in diabetic neuropathic patients with high foot pressures.high plantar pressures and painless trauma are associated with the development of foot ulcers in diabetic patients. padded hosiery has been reported to reduce plantar pressures in patients at risk of ulceration. using the optical pedobarograph we have studied 10 patients who regularly wore experimental padded hosiery for 6 months. the hosiery continued to provide substantial and significant reduction in peak forefoot pressures at 3 months (mean reduction 15.5%, p less than 0.01) and 6 months (17 ...20072140084
[characteristics of the pharmacodynamics of alpha adrenergic receptor blockader pratsiol in various age groups]. 20072892964
[characteristics of the pharmacodynamics of alpha adrenergic receptor blockader pratsiol in various age groups]. 20072892964
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