Publications

isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources.	bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ...	2015	27682080
alanine-scanning mutational analysis of durancin gl reveals residues important for its antimicrobial activity.	durancin gl is a novel class iia bacteriocin with 43 residues produced by enterococcus durans 41d. this bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several listeria monocytogenes strains, including nisin-resistant l. monocytogenes nr30. a systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin gl residues important for antimicrobial activity and specificity. results showed that three mutati ...	2015	26168032
genetic diversity and some aspects of antimicrobial activity of lactic acid bacteria isolated from goat milk.	lactic acid bacteria (lab, n = 57) were previously obtained from raw goat milk, identified as lactococcus spp. (n = 24) and enterococcus spp. (n = 33), and characterized as bacteriocinogenic. fingerprinting by pulsed field gel electrophoresis (pfge) demonstrated high genetic diversity, and 30 strains were selected and exhibited strong antimicrobial activity against 46 target strains (lab, spoilage, and foodborne pathogens). six strains (lactococcus lactis: glc03 and glc05; and enterococcus duran ...	2015	25637509
effects of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases on intracellular survival in dictyostelium discoideum and mammalian cell lines.	some bacterial pathogens produce chitinases as virulence factors during host infection. the molecular target of such enzymes in non-chitinous hosts remains uncertain. we studied the importance of listeria monocytogenes egd-e and salmonella enterica ser. typhimurium lt2 chitinases for intracellular survival in dictyostelium discoideum, and for salmonella, also infection of mammalian cell lines, and a mouse model. the salmonella chitinase did not contribute significantly to infection of d. discoid ...	2015	25908871
in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice.	lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ...	2015	26142853
an assessment of the human health impact of seven leading foodborne pathogens in the united states using disability adjusted life years.	we explored the overall impact of foodborne disease caused by seven leading foodborne pathogens in the united states using the disability adjusted life year (daly). we defined health states for each pathogen (acute illness and sequelae) and estimated the average annual incidence of each health state using data from public health surveillance and previously published estimates from studies in the united states, canada and europe. these pathogens caused about 112 000 dalys annually due to foodborn ...	2015	25633631
development and application of loop-mediated isothermal amplification assays based on its-1 for rapid detection of toxoplasma gondii in pork.	the loop-mediated isothermal amplification (lamp) assay is a novel method that rapidly amplifies dna with high specificity and sensitivity under isothermal conditions. in this study, we established a lamp assay with six primers targeting a highly conserved region of toxoplasma gondii its-1 sequence. the amplification protocol completes within 30 min under isothermal condition in a 65°c water bath while specificity tests confirmed no cross-reactivity with dna templates of neospora caninum, eimeri ...	2015	25624074
comparison of desiccation tolerance among listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii in powdered infant formula.	bacterial pathogens such as listeria monocytogenes, escherichia coli o157:h7, salmonella enterica, and cronobacter sakazakii have demonstrated long-term survival in/on dry or low-water activity (aw) foods. however, there have been few comparative studies on the desiccation tolerance among these bacterial pathogens separately in a same food matrix. in the present study, the survival kinetics of the four bacterial pathogens separately inoculated onto powdered infant formula as a model low-aw food ...	2015	25581184
development and validation of a stochastic model for potential growth of listeria monocytogenes in naturally contaminated lightly preserved seafood.	a new stochastic model for the simultaneous growth of listeria monocytogenes and lactic acid bacteria (lab) was developed and validated on data from naturally contaminated samples of cold-smoked greenland halibut (csgh) and cold-smoked salmon (css). during industrial processing these samples were added acetic and/or lactic acids. the stochastic model was developed from an existing deterministic model including the effect of 12 environmental parameters and microbial interaction (o. mejlholm and p ...	2015	25500393
modeling transfer of escherichia coli o157:h7 and listeria monocytogenes during preparation of fresh-cut salads: impact of cutting and shredding practices.	cutting and shredding of leafy vegetables increases the risk of cross contamination in household settings. the distribution of escherichia coli o157:h7 and listeria monocytogenes transfer rates (tr) between cutting knives and lettuce leaves was investigated and a semi-mechanistic model describing the bacterial transfer during consecutive cuts of leafy vegetables was developed. for both pathogens the distribution of log10trs from lettuce to knife was towards low values. conversely log10trs from k ...	2015	25500391
investigating boundaries of survival, growth and expression of genes associated with stress and virulence of listeria monocytogenes in response to acid and osmotic stress.	the objective of this study was to correlate the relative transcription of listeria monocytogenes (strains c5, 6179) stress- (gad2, sigb) and virulence- (prfa) associated genes following habituation at twenty-five ph (4.8, 5.0, 5.2, 5.5, 6.4) and nacl (2, 4, 6, 8, 10% w/v) combinations at 7 °c, with the survival against subsequent exposure to severe acid stress (ph 2.0 at 37 °c). our findings pointed out the inter-strain variation governing growth inhibiting conditions (ph ≤5.0 and nacl ≥6%), wh ...	2015	25500389
comparison of individual-based modeling and population approaches for prediction of foodborne pathogens growth.	individual-based modeling (ibm) approach combined with the microenvironment modeling of vacuum-packed cold-smoked salmon was more effective to describe the variability of the growth of a few listeria monocytogenes cells contaminating irradiated salmon slices than the traditional population models. the ibm approach was particularly relevant to predict the absence of growth in 25% (5 among 20) of artificially contaminated cold-smoked salmon samples stored at 8 °c. these results confirmed similar o ...	2015	25500386
preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.	present study illustrates the preparation of chitin nanofibrils (cnf) reinforced carrageenan nanocomposite films by the solution-casting technique. cnf was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication. ftir result demonstrated that the chemical structure of chitin had not changed after acid hydrolysis. however, the crystalinity of cnf was found to be higher than chitin. the crystallite size of chitin and cnf was 4.73 and 6.27 nm, respectively. the c ...	2015	25498660
essential oils from herbs against foodborne pathogens in chicken sausage.	consumption of chicken meat and its products, especially sausage, have increased in recent years. however, this product is susceptible to microbial contamination during manufacturing, which compromises its shelf life. the flavoring and preservative activities of essential oils (eo) have been recognized and the application of these antimicrobial agents as natural active compounds in food preservation has shown promise. the aim of this study was to evaluate the effect of ocimum basilicum and origa ...	2015	25492235
design and characterization of novel recombinant listeriolysin o-protamine fusion proteins for enhanced gene delivery.	to improve the efficiency of gene delivery for effective gene therapy, it is essential that the vector carries functional components that can promote overcoming barriers in various steps leading to the transport of dna from extracellular to ultimately nuclear compartment. in this study, we designed genetically engineered fusion proteins as a platform to incorporate multiple functionalities in one chimeric protein. prototypes of such a chimera tested here contain two domains: one that binds to dn ...	2015	25521817
schistosoma mansoni soluble egg antigens enhance t cell responses to a newly identified hiv-1 gag h-2b epitope.	schistosome infection induces significant t helper type 2 (th2) and anti-inflammatory immune responses and has been shown to negatively impact vaccine efficacy. our goal was to determine if the administration of schistosome soluble egg antigens (sea) would negatively influence the induction of cytotoxic t lymphocyte (ctl) and th1-type t cell responses to an hiv candidate vaccine in the th1-biased c57bl/6 mouse strain. initial experiments failed, as we were unable to detect any response to the de ...	2015	25520148
a prl mutation in secy suppresses secretion and virulence defects of listeria monocytogenes seca2 mutants.	the bulk of bacterial protein secretion occurs through the conserved secy translocation channel that is powered by seca-dependent atp hydrolysis. many gram-positive bacteria, including the human pathogen listeria monocytogenes, possess an additional nonessential specialized atpase, seca2. seca2-dependent secretion is required for normal cell morphology and virulence in l. monocytogenes; however, the mechanism of export via this pathway is poorly understood. l. monocytogenes seca2 mutants form ro ...	2015	25535272
exploring specific primers targeted against different genes for a multiplex pcr for detection of listeria monocytogenes.	the efficacy of six different sets of primers targeted against 16s rrna and virulence genes such as 'iap', 'hly' and 'prf' was evaluated in separate pcr assays. the primer pairs targeted against 16s rrna resulted into amplification of 1.2 kb pcr product. however, sets of primers targeted against different regions of 'iap' produced 371 and 660 bp pcr products, respectively. the primer pair targeted against 'prf' gene could produce 508 bp product. three primer pairs targeted against different regi ...	2015	28324291
mining of novel species-specific primers for pcr detection of listeria monocytogenes based on genomic approach.	listeria monocytogenes in contaminated food is considered as a serious health threat for consumers due to its high mortality rate. the objective of this study was to obtain novel species-specific target-genes and primers for the molecular detection of l. monocytogenes using a comparative genomic approach. by comparative analysis of l. monocytogenes and non-l. monocytogenes genome sequences in the genbank database with blast program, 26 specific target sequences were used as candidates and the pr ...	2015	26354019
combination of zinc and all-trans retinoic acid promotes protection against listeria monocytogenes infection.	zinc (zn) is the second most abundant transition metal after iron. it plays a vital role in living organisms and affects multiple aspects of the immune system. all-trans retinoic acid (atra) is an isomeric form of the vitamin a or retinol. it possesses the greatest biological activity of vitamin a. vitamin a and related retinoids influence many aspects of immunity. in this study, we demonstrated that treatment with a combination of zn and atra contributes to host resistance against infection by ...	2015	26351852
effect of lemongrass essential oil vapors on microbial dynamics and listeria monocytogenes survival on rocket and melon stored under different packaging conditions and temperatures.	the aim of the present study was to examine the effect of lemongrass essential oil vapors on the dynamics of surface microbiota and l. monocytogenes growth on rocket and melon under different packaging conditions and storage temperature. for that purpose, rocket and melon were placed on expanded polystyrene (eps) trays, sprayed with l. monocytogenes to a population of 4.5-5.0 log cfu·g(-1), packaged using microperforated oriented polypropylene (opp) film in either air or microperforated active m ...	2015	27682104
fumaric acid and slightly acidic electrolyzed water inactivate gram positive and gram negative foodborne pathogens.	sanitizing effectiveness of slightly acidic electrolyzed water (saew) and fumaric acid (fa) at different dipping temperatures (25-60 °c), times (1-5 min), and concentrations (5-30 ppm for saew and 0.125%-0.5% for fa) on pure cultures of two gram positive pathogens staphylococcus aureus (sa) and listeria monocytogenes (lm) and two gram negative pathogens escherichia coli o157:h7 (ec) and salmonella typhimurium (st) was evaluated. fa (0.25%) showed the strongest sanitizing effect, demonstrating co ...	2015	27682077
identification of multiple bacteriocins in enterococcus spp. using an enterococcus-specific bacteriocin pcr array.	twenty-two bacteriocin-producing enterococcus isolates obtained from food and animal sources, and demonstrating activity against listeria monocytogenes, were screened for bacteriocin-related genes using a bacteriocin pcr array based on known enterococcal bacteriocin gene sequences in the ncbi genbank database. the 22 bacteriocin-positive (bac+) enterococci included en. durans (1), en. faecalis (4), en. faecium (12), en. hirae (3), and en. thailandicus (2). enterocin a (enta), enterocins mr10a an ...	2015	27682075
exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of the honeycomb-like morphotype.	listeria monocytogenes is involved in food-borne illness with a high mortality rate. the persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. while most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by l. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. using a hig ...	2015	25548046
antimicrobial and mosquitocidal activity of microbial synthesized silver nanoparticles.	microbial synthesis of nanoparticles is a green approach that interconnects nanotechnology and microbial biotechnology. here, we synthesized the silver nanoparticles (agnps) using bacterial strains of listeria monocytogenes, bacillus subtilius and streptomyces anulatus. we tested the efficacy of agnps against the larvae, pupae and adults of anopheles stephensi and culex quinquefasciatus. we have also investigated the antifungal activity of agnps against the soil keratinophilic fungus of chrysosp ...	2015	25544704
hplc/ms analysis of polyphenols, antioxidant and antimicrobial activities of artabotrys hildebrandtii o. hffm. extracts.	the purpose of this work was to evaluate chemical constituents, antioxidant and antimicrobial activities of artabotrys hildebrandtii, an endemic medicinal plant from madagascar. ethanol extracts from the leaves and stem bark were tested to evaluate dpph free radical scavenging, using butylated hydroxytoluene and quercetin as standard antioxidants. an high-performance liquid chromatography/mass spectrometry method was developed to investigate the presence of phenolic compounds in the studied samp ...	2015	25679267
protective dendritic cell responses against listeriosis induced by the short form of the deubiquitinating enzyme cyld are inhibited by full-length cyld.	the deubiquitinating enzyme cyld is an important tumor suppressor and inhibitor of immune responses. in contrast to full-length cyld, the immunological function of the naturally occurring short splice variant of cyld (scyld) is insufficiently described. previously, we showed that dcs, which lack full-length cyld but express scyld, exhibit augmented nf-κb and dc activation. to explore the function of scyld in infection, we investigated whether dc-specific scyld regulates the pathogenesis of liste ...	2015	25675948
a case report of listeria monocytogenes meningoencephalitis in general hospital "dr radivoj simonovir" sombor.	listeria monocytogenes is one of the most common causes of bacterial central nervous system infections in adults. it often affects immunicompromised and elderly patients. even with appropriate antimicrobial treatment, mortality due to listeria monocytogenes meningoencephalitis is among the highest of all causes of bacterial central nervous system infections.	2015	25675833
persistent and transient listeria monocytogenes strains from retail deli environments vary in their ability to adhere and form biofilms and rarely have inla premature stop codons.	based on recent risk assessments, up to 83% of listeriosis cases from deli meat in the united states are predicted to be from ready-to-eat deli meats contaminated during processing at retail grocery stores. listeria monocytogenes is known to use sanitizer tolerance and biofilm formation to survive, but interplay of these mechanisms along with virulence potential and persistence mechanisms specific to deli environments had yet to be elucidated. in this study, 442 isolates from food and nonfood co ...	2015	25569840
the tyrosine kinase itk suppresses cd8+ memory t cell development in response to bacterial infection.	vaccine efficacy depends on strong long-term development of immune memory and the formation of memory cd8(+) t cells is critical for recall responses to infection. upon antigen recognition by naïve t cells, the strength of the tcr signal influences the subsequent effector and memory cells differentiation. here, we have examined the role of itk, a tyrosine kinase critical for tcr signaling, in cd8(+) effector and memory t cell differentiation during listeria monocytogenes infection. we found that ...	2015	25567129
listeria monocytogenes has a functional chitinolytic system and an active lytic polysaccharide monooxygenase.	chitinases and chitin-active lytic polysaccharide monooxygenases (lpmos) are most commonly associated with chitin metabolism, but are also reported as virulence factors in pathogenic bacteria. listeria monocytogenes, a well-known virulent bacterium, possesses two chitinases (chia and chib) and a multi-modular lytic polysaccharide monooxygenase (lmlpmo10). these enzymes have been related to virulence and their role in chitin metabolism is poorly understood. it is thus of interest to functionally ...	2015	25565565
suppression of toll-like receptor-mediated innate immune responses at the ocular surface by the membrane-associated mucins muc1 and muc16.	membrane-associated mucins (mams) expressed on the ocular surface epithelium form a dense glycocalyx that is hypothesized to protect the cornea and conjunctiva from external insult. in this study, the hypothesis that the mams muc1 and muc16, expressed on the apical surface of the corneal epithelium, suppress toll-like receptor (tlr)-mediated innate immune responses was tested. using an in vitro model of corneal epithelial cells that are cultured to express mams, we show that reduced expression o ...	2015	25563498
investigating the therapeutic potential of herbal leads against drug resistant listeria monocytogenes by computational virtual screening and in vitro assays.	listeria monocytogenes, a gram-positive opportunistic food-borne pathogen, naturally resistant to many antibiotics and acquired resistance may be a concern in the nearer future. hence, there is a scope for screening of novel therapeutic agents and drug targets, toward the treatment of fatal listeria infections. the seca homologs, seca1 and seca2 are the essential components of the general secretion (sec) pathway, a specialised protein export system, present in l. monocytogenes. this study evalua ...	2015	25562366
molecular serotyping and pathogenic potential of listeria monocytogenes isolated from milk and milk products in tamil nadu, india.	listeria monocytogenes, an important bacterial pathogen, is responsible for foodborne illnesses worldwide. examination of food samples for the presence of l. monocytogenes and assessment of their pathogenicity is usually an effective strategy in the prevention of listeriosis. in the present study, we have tested 307 samples of milk and milk products from various places in tamil nadu, india for the presence of l. monocytogenes using iso 11290 and u.s. food and drug administration bacteriological ...	2015	25793931
complete genome sequence of the persistent listeria monocytogenes strain r479a.	the complete genome sequence of the persistent listeria monocytogenes strain r479a isolated from smoked salmon in denmark and belonging to lineage ii, serovar 1/2a, and multilocus sequence type 8 (st8) is presented here.	2015	25792065
sensitive enumeration of listeria monocytogenes and other listeria species in various naturally contaminated matrices using a membrane filtration method.	for the enumeration of listeria monocytogenes (l. monocytogenes) in food, a sensitive enumeration method has been recently developed. this method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate l. monocytogenes. an evaluation of this method was performed with several categories of foods naturally contaminated with l. monocytogenes. the results obtained with this technique were compared with those obtained from the mod ...	2015	25791005
a novel gene, lstc, of listeria monocytogenes is implicated in high salt tolerance.	listeria monocytogenes, causative agent of human listeriosis, has been isolated from a wide variety of foods including deli meats, soft cheeses, cantaloupes, sprouts and canned mushrooms. standard control measures for restricting microbial growth such as refrigeration and high salt are often inadequate as l. monocytogenes grows quite well in these environments. in an effort to better understand the genetic and physiological basis by which l. monocytogenes circumvents these controls, a transposon ...	2015	25790994
enhancing the antibacterial effect of 461 and 521 nm light emitting diodes on selected foodborne pathogens in trypticase soy broth by acidic and alkaline ph conditions.	light emitting diodes (leds) with their antibacterial effect present a novel method for food preservation. this effect may be influenced by environmental conditions such as the ph of the food contaminated by the pathogen. thus, it is necessary to investigate the influence of ph on the antibacterial effect of leds before their application to real food matrices. escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in trypticase soy broth were illuminated using 10-w 461 (22.1 ...	2015	25790991
effects of oxygen-depleted atmospheres on survival and growth of listeria monocytogenes on fresh-cut iceberg lettuce stored at mild abuse commercial temperatures.	the effects of oxygen-depleted atmospheres, 0.25% o2+12% co2 (balance n2) and 2% o2 + 6% co2 (balance n2), on growth of listeria monocytogenes on fresh-cut iceberg lettuce were determined. the study was carried out at mild abuse temperatures using controlled atmosphere chambers. during storage at a constant temperature of 7 °c, growth was enhanced at the lower oxygen level of 0.25% o2 by day 10. over 17 days of storage at temperatures designed to mimic mild abuse commercial conditions, there wer ...	2015	25790986
notes from the field: listeriosis associated with stone fruit--united states, 2014.	on july 19, 2014, a packing company in california (company a) voluntarily recalled certain lots of stone fruits, including whole peaches, nectarines, plums, and pluots, because of concern about contamination with listeria monocytogenes based on internal company testing. on july 31, the recall was expanded to cover all fruit packed at their facility during june 1-july 17. after the initial recall, clinicians, state and local health departments, cdc, and the food and drug administration (fda) rece ...	2015	25789745
evaluation of transcription levels of inla, inlb, hly, bsh and prfa genes in listeria monocytogenes strains using quantitative reverse-transcription pcr and ability of invasion into human caco-2 cells.	listeria monocytogenes virulence depends on the activity of well-characterized virulence factors. in this study, transcription levels of inla, inlb, hly, bsh and prfa genes in l. monocytogenes strains, and the ability of invasion into caco-2 cells were investigated. serotyping, multiplex-pcr for serovar identification and restriction fragment analysis of inla were performed. transcription levels and invasiveness were evaluated by quantitative reverse-transcription pcr and by in vitro assays, res ...	2015	25673285
mir-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function.	microrna (mir)-mediated regulation of protein abundance is a pervasive mechanism of directing cellular processes. the well-studied and abundant mir-182 has previously been implicated in many aspects of t cell function, dna repair, and cancer. in this study, we show that mir-182 is the most highly induced mir in b cells undergoing class-switch recombination. to elucidate the requirement of mir-182 in lymphocyte function, we extensively characterized mice with a targeted deletion of mir182. we sho ...	2015	25672759
organelle targeting during bacterial infection: insights from listeria.	listeria monocytogenes, a facultative intracellular bacterium responsible for severe foodborne infections, is now recognized as a multifaceted model in infection biology. comprehensive studies of the molecular and cellular basis of the infection have unraveled how the bacterium crosses the intestinal and feto-placental barriers, invades several cell types in which it multiplies and moves, and spreads from cell to cell. interestingly, although listeria does not actively invade host cell organelle ...	2015	25670529
listeria monocytogenes varies among strains to maintain intracellular ph homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry.	listeria monocytogenes, a food-borne pathogen, has the capacity to maintain intracellular ph (phi) homeostasis in acidic environments, but the underlying mechanisms remain elusive. here, we report a simple microplate-based fluorescent method to determine phi of listerial cells that were prelabeled with the fluorescent dye carboxyfluorescein diacetate n-succinimidyl ester and subjected to acid stress. we found that l. monocytogenes responds differently among strains toward organic and inorganic a ...	2015	25667585
the class ii phosphatidylinositol 3-phosphate kinase pik3c2a promotes shigella flexneri dissemination through formation of vacuole-like protrusions.	intracellular pathogens such as shigella flexneri and listeria monocytogenes achieve dissemination in the intestinal epithelium by displaying actin-based motility in the cytosol of infected cells. as they reach the cell periphery, motile bacteria form plasma membrane protrusions that resolve into vacuoles in adjacent cells, through a poorly understood mechanism. here, we report on the role of the class ii phosphatidylinositol 3-phosphate kinase pik3c2a in s. flexneri dissemination. time-lapse mi ...	2015	25667265
g0s2 modulates homeostatic proliferation of naïve cd8⁺ t cells and inhibits oxidative phosphorylation in mitochondria.	since its discovery, diverse functions have been attributed to the g0/g1 switch gene 2 (g0s2), from lipid metabolism to control of cell proliferation. our group showed for the first time that g0s2 promotes quiescence in hematopoietic stem cells by interacting with and retaining nucleolin around the nucleus. herein, we report the role of g0s2 in the differentiation and function of cd8(+) t cells examined in mice with an embryonic deletion of the g0s2 gene. g0s2 expression in naïve cd8(+) t cells ...	2015	25666096
efficacies of nisin a and nisin v semipurified preparations alone and in combination with plant essential oils for controlling listeria monocytogenes.	the food-borne pathogenic bacterium listeria is known for relatively low morbidity and high mortality rates, reaching up to 25 to 30%. listeria is a hardy organism, and its control in foods represents a significant challenge. many naturally occurring compounds, including the bacteriocin nisin and a number of plant essential oils, have been widely studied and are reported to be effective as antimicrobial agents against spoilage and pathogenic microorganisms. the aim of this study was to investiga ...	2015	25662980
listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-gmp-dependent regulation.	elevated levels of the second messenger c-di-gmp activate biosynthesis of an unknown exopolysaccharide (eps) in the food-borne pathogen listeria monocytogenes. this eps strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety. we analyzed the potential phylogenetic origin of this eps, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and id ...	2015	25662512
synergy among thymol, eugenol, berberine, cinnamaldehyde and streptomycin against planktonic and biofilm-associated food-borne pathogens.	essential oils have been found to exert antibacterial, antifungal, spasmolytic, and antiplasmodial activity and therapeutic effect in cancer treatment. in this study, the antibacterial activities of four main essential oils' components (thymol (thy), eugenol (eug), berberine (ber), and cinnamaldehyde (cin)) were evaluated against two food-borne pathogens, listeria monocytogenes and salmonella typhimurium, either alone or in combination with streptomycin. checkerboard assay demonstrated that thy ...	2015	25661823
a gold glyco-nanoparticle carrying a listeriolysin o peptide and formulated with advax™ delta inulin adjuvant induces robust t-cell protection against listeria infection.	in the search for an effective vaccine against the human pathogen, listeria monocytogenes (listeria), gold glyconanoparticles (gnp) loaded with a listeriolysin o peptide llo91-99 (gnp-llo) were used to immunise mice, initially using a dendritic cell (dc) vaccine approach, but subsequently using a standard parenteral immunisation approach. to enhance vaccine immunogenicity a novel polysaccharide adjuvant based on delta inulin (advax™) was also co-formulated with the gnp vaccine. confirming previo ...	2015	25659269
pfge standard operating procedures for listeria monocytogenes: harmonizing the typing of food and clinical strains in europe.	listeria monocytogenes is a foodborne pathogen responsible for a severe disease known as listeriosis. the european centre for disease prevention and control (ecdc) coordinates a network of national public health laboratories (nphls) in charge of typing clinical strains. in food, it is the european union reference laboratory for l. monocytogenes (eurl lm), which manages a network of national reference laboratories (nrls). a pulsed-field gel electrophoresis (pfge) standard operating procedure (eur ...	2015	25658711
whole-genome sequence of listeria monocytogenes strains from clinical and environmental samples from varanasi, india.	we present here the whole-genome sequences of listeria monocytogenes from ganges river water, agricultural soil, and human clinical samples from varanasi, india, which will be used for a comparative analysis.	2015	25657276
draft genome sequence of listeria monocytogenes lm201, isolated from foodstuff.	listeria monocytogenes is a facultative intracellular foodborne pathogen that can cause listeriosis in humans and animals. l. monocytogenes lm201 was isolated from foodstuff. the draft genome sequence of strain lm201 provides the genetic basis for the application of this strain in biotechnological vaccine production.	2015	25657267
characterization of polar lipids of listeria monocytogenes by hcd and low-energy cad linear ion-trap mass spectrometry with electrospray ionization.	listeria monocytogenes (l. monocytogenes) is a facultative, gram-positive, food-borne bacterium, which causes serious infections. although it is known that lipids play important roles in the survival of listeria, the detailed structures of these lipids have not been established. in this contribution, we described linear ion-trap multiple-stage mass spectrometric approaches with high-resolution mass spectrometry toward complete structural analysis including the identities of the fatty acid substi ...	2015	25656850
listeria monocytogenes as a cause of spontaneous bacterial peritonitis: a rare entity.	listeria is an uncommon cause of spontaneous bacterial peritonitis (sbp) in the united states. listeria should be suspected as a cause of sbp when the patient has diphtheria-like organisms on ascitic/blood cultures, iron overload/hemochromatosis, exposure to farm animals, or poor response to empiric therapy within 48-72 h. diagnosis of sbp is made if the ascitic fluid shows polymorphonuclear cell count >250 cells/mm(3) without an intra-abdominal source of infection. ampicillin with or without an ...	2015	25656671
microfluidic filtration and extraction of pathogens from food samples by hydrodynamic focusing and inertial lateral migration.	detecting pathogenic bacteria in food or other biological samples with lab-on-a-chip (loc) devices requires several sample preparation steps prior to analysis which commonly involves cleaning complex sample matrices of large debris. this often underestimated step is important to prevent these larger particles from clogging devices and to preserve initial concentrations when loc techniques are used to concentrate or isolate smaller target microorganisms for downstream analysis. in this context, w ...	2015	25653055
septicemic listeriosis in wild hares from saskatchewan, canada.	the bacterium listeria monocytogenes causes disease in a wide variety of mammals including rabbits and hares. we describe naturally acquired metritis and septicemic listeriosis in wild female hares from saskatchewan, canada. between april 2012 and july 2013, two white-tailed jackrabbits (lepus townsendii) and a snowshoe hare (lepus americanus) were presented to the veterinary medical centre at the western college of veterinary medicine, saskatoon, saskatchewan, canada with nonspecific neurologic ...	2015	25647601
contribution of the multiple type i signal peptidases to the secretome of listeria monocytogenes: deciphering their specificity for secreted exoproteins by exoproteomic analysis.	as commonly seen in monoderm bacteria, listeria monocytogenes possesses multiple membrane-bound signal peptidases of type i (spases i) called sipx, sipy and sipz. in order to decipher their respective contribution in an integrated and global view, the complement of the secretome corresponding to the exoproteome was resolved by two-dimensional gel electrophoresis (2-de). this was performed for l. monocytogenes sipx(-), sipy(-), sipz(-) single mutants, as well as for δsipxy and δsipyz double mutan ...	2015	25637307
src-dependent tyrosine phosphorylation of non-muscle myosin heavy chain-iia restricts listeria monocytogenes cellular infection.	bacterial pathogens often interfere with host tyrosine phosphorylation cascades to control host responses and cause infection. given the role of tyrosine phosphorylation events in different human infections and our previous results showing the activation of the tyrosine kinase src upon incubation of cells with listeria monocytogenes, we searched for novel host proteins undergoing tyrosine phosphorylation upon l. monocytogenes infection. we identify the heavy chain of the non-muscle myosin iia (n ...	2015	25635050
structure and mechanism of the caseinolytic protease clpp1/2 heterocomplex from listeria monocytogenes.	listeria monocytogenes is a devastating bacterial pathogen. its virulence and intracellular stress tolerance are supported by caseinolytic protease p (clpp), an enzyme that is conserved among bacteria. l. monocytogenes expresses two clpp isoforms that are only distantly related by sequence and differ in catalysis, oligomerization, active-site composition, and n-terminal interaction sites for associated aaa(+) chaperones. the crystal structure of the clpp1/2 heterocomplex from l. monocytogenes wa ...	2015	25630955
empirical antibiotic cover for listeria monocytogenes infection beyond the neonatal period: a time for change?		2015	25628458
shelf life determination of sliced portuguese traditional blood sausage--morcela de arroz de monchique through microbiological challenge and consumer test.	morcela de arroz (ma) is a ready-to-eat blood and rice cooked sausage produced with pork, blood, rice, and seasonings, stuffed in natural casing and cooked above 90 °c/30 min. it is commercialized whole, not packed, with a restricted shelf life (1 wk/0 to 5 °c). the objective of this work was to establish sliced ma shelf life considering both the behavior of l. monocytogenes through a microbiological challenge test (mct) and the consumer acceptability of ma stored: vacuum packed (vp), modified a ...	2015	25627176
pi3-kinase activation is critical for host barrier permissiveness to listeria monocytogenes.	invasion of nonphagocytic cells, a critical property of listeria monocytogenes (lm) that enables it to cross host barriers, is mediated by the interaction of two bacterial surface proteins, inla and inlb, with their respective receptors e-cadherin and c-met. although inla-e-cadherin interaction is necessary and sufficient for lm crossing of the intestinal barrier, both inla and inlb are required for lm crossing of the placental barrier. the mechanisms underlying these differences are unknown. ph ...	2015	25624443
impact of the contamination level and the background flora on the growth of listeria monocytogenes in ready-to-eat diced poultry.	the food safety criteria that have been incorporated in european regulation (ec) no2073/2005 (official journal of the european union l, 338, 2005, 1), for listeria monocytogenes in ready-to eat (rte) foods, specify a maximum allowable concentration of 100 cfu g(-1) or ml(-1) . some factors such as ph, salt and modified atmosphere packaging (map) are used to prevent the growth of l. monocytogenes in order to comply with the limit. interactions between background microflora (bm) and l. monocytogen ...	2015	25619748
type i interferons in infectious disease.	type i interferons (ifns) have diverse effects on innate and adaptive immune cells during infection with viruses, bacteria, parasites and fungi, directly and/or indirectly through the induction of other mediators. type i ifns are important for host defence against viruses. however, recently, they have been shown to cause immunopathology in some acute viral infections, such as influenza virus infection. conversely, they can lead to immunosuppression during chronic viral infections, such as lympho ...	2015	25614319
risk-based control of food-borne pathogens listeria monocytogenes and salmonella enterica in the italian fermented sausages cacciatore and felino.	fermentation is the most important killing step during production of fermented meats to eliminate food-borne pathogens. the objective was to evaluate whether the food-borne pathogens listeria monocytogenes and salmonella enterica may survive during the production of two italian fermented sausages. sausage batter was inoculated with five strains of l. monocytogenes or s. enterica (ca. 10(5)-10(6) cfu/g) and their kinetic behavior was monitored during production. both pathogens survived relatively ...	2015	25612557
pregnancy related listeriosis: treatment and control.	listeriosis during pregnancy usually presents as an unremarkable febrile illness in the mother but can be fatal for the fetus and newborn. reliable laboratory testing for early diagnosis is lacking. serological antibody tests and bacteriological stool tests are not helpful since listeria-specific antibodies and stool cultures yielding the organism can be found in healthy pregnant women. because early diagnosis is difficult, diagnosis is usually made by culturing the pathogen from blood, cerebros ...	2015	25604158
proteome mining for drug target identification in listeria monocytogenes strain egd-e and structure-based virtual screening of a candidate drug target penicillin binding protein 4.	we used a combination of in-silico approaches to identify 168 promising drug targets in the proteome of a multidrug-resistant listeria monocytogenes strain; of these, one (pbp4) was particularly promising. virtual screening using it, followed by reverse docking, revealed four compounds namely nci524121, cap332797, nci524136 and zinc00518462 as good multi-target leads.	2015	25601791
listeria phage and phage tail induction triggered by components of bacterial growth media (phosphate, licl, nalidixic acid, and acriflavine).	the detection of listeria monocytogenes from food is currently carried out using a double enrichment. for the iso methodology, this double enrichment is performed using half-fraser and fraser broths, in which the overgrowth of l. innocua can occur in samples where both species are present. in this study, we analyzed the induction of phages and phage tails of listeria spp. in these media and in two brain heart infusion (bhi) broths (bhim [biomérieux] and bhik [biokar]) to identify putative effect ...	2015	25595760
complete genome sequence of listeria monocytogenes ntsn, a serovar 4b and animal source strain.	listeria monocytogenes is an important foodborne pathogen that causes infections in humans and animals and has a high mortality rate. the complete genome sequence of l. monocytogenes strain ntsn, a highly virulent and serovar 4b strain isolated from the brains of sheep in jiangsu province, china, is presented here.	2015	25593254
pathogen-expanded cd11b+ invariant nkt cells feedback inhibit t cell proliferation via membrane-bound tgf-β1.	natural killer t cells (nkt cells) are effector cells, but also regulator of immune response, which either promote or suppress immune response through production of different cytokines. however, the subsets of nkt cells with definite phenotype and regulatory function need to be further identified. furthermore, the mechanisms for nkt cells to regulate immune response remain to be fully elucidated. here we identified cd11b(+) invariant nkt (cd11b(+) inkt) cells as a new subset of regulatory nkt ce ...	2015	25592391
listeria arpj gene modifies t helper type 2 subset differentiation.	although the t-cell subset differentiation pathway has been characterized extensively from the view of host gene regulation, the effects of genes of the pathogen on t-cell subset differentiation during infection have yet to be elucidated. especially, the bacterial genes that are responsible for this shift have not yet been determined.	2015	25589336
listeria monocytogenes batch culture growth response to metabolic inhibitors.	in certain environments nutrient and energy sources available to microorganisms can be limited. foodborne pathogens must efficiently adapt in order to be successfully transmitted through the food chain to their hosts. for the intracellular foodborne pathogen listeria monocytogenes, little is known regarding its response to nutrient/energy-limiting conditions. the alternative stress responsive sigma factor σ(b) has been reported to contribute to survival under specific stresses. therefore, the ef ...	2015	25587784
genotypic analyses and virulence characterization of listeria monocytogenes isolates from crayfish (procambarus clarkii).	listeria monocytogenes is a foodborne pathogen that can cause invasive illness in humans and farm animals. it is frequently isolated from dairy products and poultry. however, there have been few literatures on the genetic diversity and virulence potential of l. monocytogenes from freshwater animal. thirty-nine l. monocytogenes strains from crayfish were isolated and identified in this study. molecular subtyping and polymorphism of each isolate were analyzed by multilocus sequence typing (mlst). ...	2015	25586079
safety and survival with gvax pancreas prime and listeria monocytogenes-expressing mesothelin (crs-207) boost vaccines for metastatic pancreatic cancer.	gvax pancreas, granulocyte-macrophage colony-stimulating factor-secreting allogeneic pancreatic tumor cells, induces t-cell immunity to cancer antigens, including mesothelin. gvax is administered with low-dose cyclophosphamide (cy) to inhibit regulatory t cells. crs-207, live-attenuated listeria monocytogenes-expressing mesothelin, induces innate and adaptive immunity. on the basis of preclinical synergy, we tested prime/boost vaccination with gvax and crs-207 in pancreatic adenocarcinoma.	2015	25584002
evaluation of aqueous and alcohol-based quaternary ammonium sanitizers for inactivating salmonella spp., escherichia coli o157:h7, and listeria monocytogenes on peanut and pistachio shells.	this study evaluated the efficacy of aqueous (aquat) and isopropyl alcohol-based quaternary ammonium (ipquat) sanitizers for reducing salmonella spp., escherichia coli o157:h7, or listeria monocytogenes populations on peanut and pistachio shell pieces. inoculated nutshells were mixed with quat sanitizers, water, or 70% ethanol and enumerated immediately or after incubation at 30 °c for 48 h. none of the treatments had any immediate effect on salmonella or e. coli o157:h7 populations on the peanu ...	2015	25583342
application of an oregano oil nanoemulsion to the control of foodborne bacteria on fresh lettuce.	although antimicrobial activities of plant essential oils are well documented, challenges remain as to their application in fresh produce due to the hydrophobic nature of essential oils. oregano oil nanoemulsions were formulated with a food-grade emulsifier and evaluated for their efficacy in inactivating the growth of foodborne bacteria on fresh lettuce. lettuce was artificially inoculated with listeria monocytogenes, salmonella typhimurium and escherichia coli o157:h7, followed by a one-minute ...	2015	25583339
listeria monocytogenes in retail delicatessens: an interagency risk assessment-model and baseline results.	the interagency risk assessment-listeria monocytogenes (lm) in retail delicatessens provides a scientific assessment of the risk of listeriosis associated with the consumption of ready-to-eat (rte) foods commonly prepared and sold in the delicatessen (deli) of a retail food store. the quantitative risk assessment (qra) model simulates the behavior of retail employees in a deli department and tracks the lm potentially present in this environment and in the food. bacterial growth, bacterial inacti ...	2015	25581188
tannin-rich pomegranate rind extracts reduce adhesion to and invasion of caco-2 cells by listeria monocytogenes and decrease its expression of virulence genes.	pomegranate rind is rich in tannins that have remarkable antimicrobial activities. this study aimed to evaluate the effects of a tannin-rich fraction from pomegranate rind (tfpr) on listeria monocytogenes virulence gene expression and on the pathogen's interaction with human epithelial cells. growth curves were monitored to determine the effect of tfpr on l. monocytogenes growth. the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide and fluorescence staining assays were used to examine ...	2015	25581187
invading slugs (arion vulgaris) can be vectors for listeria monocytogenes.	listeriosis is a frequent silage-associated disease in ruminants. the slugs arion vulgaris are invaders in gardens, vegetable crops and meadows for silage production. field and laboratory studies were conducted to clarify whether slugs could host listeria monocytogenes and thereby constitute a threat to animal feed safety.	2015	25580873
comparison of five methods for direct extraction of surface proteins from listeria monocytogenes for proteomic analysis by orbitrap mass spectrometry.	extracts of surface proteins, with minimal artifacts from contaminating cytosolic components, are highly desirable for investigating surface factors involved in the attachment and formation of biofilms by bacteria that are problematic in commercial food processing facilities. in this study, we compared the protein profiles of the food pathogen, listeria monocytogenes, recovered after applying different surface protein extraction methods compiled from the literature: trypsin-enzymatic shaving wit ...	2015	25578509
liver-resident macrophage necroptosis orchestrates type 1 microbicidal inflammation and type-2-mediated tissue repair during bacterial infection.	kupffer cells, the phagocytes of fetal origin that line the liver sinusoids, are key contributors of host defense against enteroinvasive bacteria. here, we found that infection by listeria monocytogenes induced the early necroptotic death of kupffer cells, which was followed by monocyte recruitment and an anti-bacterial type 1 inflammatory response. kupffer cell death also triggered a type 2 response that involved the hepatocyte-derived alarmin interleukin-33 (il-33) and basophil-derived interle ...	2015	25577440
bioactive properties of a propolis-based dietary supplement and its use in combination with mild heat for apple juice preservation.	this study characterizes the antioxidant and antibacterial properties of a propolis-based dietary supplement (pds) and investigates its incorporation into apple juice to decrease the intensity of the heat treatment required to inactivate 5 log10 cycles of escherichia coli o157:h7. as the source of propolis, we used a pds containing 0.2 mg/μl of propylene glycol-extracted propolis (propolis). the total phenolic content and antioxidant activity (ic50) of the pds were 82.15±3.53 mg/g and 0.055±0.00 ...	2015	25897992
listeriaphages and coagulin c23 act synergistically to kill listeria monocytogenes in milk under refrigeration conditions.	bacteriophages and bacteriocins are promising biocontrol tools in food. in this work, two listeria bacteriophages, fwllm1 and fwllm3, were assessed in combination with the bacteriocin coagulin c23 to inhibit listeria monocytogenes. preliminary results under laboratory conditions demonstrated that both antimicrobials act synergistically when they were applied in suboptimal concentrations. the combined approach was further assessed in milk contaminated with 5×10(4) cfu/ml l. monocytogenes 2000/47 ...	2015	25897991
oxygen limitation induces acid tolerance and impacts simulated gastro-intestinal transit in listeria monocytogenes j0161.	ᅟ: listeria monocytogenes is a food-borne pathogen and the causative agent of listeriosis, a severe infection to those with a pre-disposition. infections often arise through consumption of contaminated foods, where high intrinsic resistance to food processing practises permit survival and growth. several practises, including refrigeration, acidification and oxygen limitation are ineffective in controlling l. monocytogenes, therefore foods which do not undergo thermal processing, e.g. ready-to-ea ...	2015	25897327
host actin polymerization tunes the cell division cycle of an intracellular pathogen.	growth and division are two of the most fundamental capabilities of a bacterial cell. while they are well described for model organisms growing in broth culture, very little is known about the cell division cycle of bacteria replicating in more complex environments. using a d-alanine reporter strategy, we found that intracellular listeria monocytogenes (lm) spend a smaller proportion of their cell cycle dividing compared to lm growing in broth culture. this alteration to the cell division cycle ...	2015	25892235
selection and characterization of phage-resistant mutant strains of listeria monocytogenes reveal host genes linked to phage adsorption.	listeria-infecting phages are readily isolated from listeria-containing environments, yet little is known about the selective forces they exert on their host. here, we identified that two virulent phages, lp-048 and lp-125, adsorb to the surface of listeria monocytogenes strain 10403s through different mechanisms. we isolated and sequenced, using whole-genome sequencing, 69 spontaneous mutant strains of 10403s that were resistant to either one or both phages. mutations from 56 phage-resistant mu ...	2015	25888172
biological effects of listeriolysin o: implications for vaccination.	listeriolysin o (llo) is a thiol-activated cholesterol-dependent pore-forming toxin and the major virulence factor of listeria monocytogenes (lm). extensive research in recent years has revealed that llo exerts a wide array of biological activities, during the infection by lm or by itself as recombinant antigen. the spectrum of biological activities induced by llo includes cytotoxicity, apoptosis induction, endoplasmic reticulum stress response, modulation of gene expression, intracellular calci ...	2015	25874208
antimicrobial coatings with dual cationic and n-halamine character: characterization and biocidal efficacy.	a method to prepare an antimicrobial coating for food-handling materials is reported. alternating layers of branched polyethylenimine and styrene maleic anhydride copolymer were applied onto the surface of polypropylene. the resulting coatings had low surface energy and presented enhanced antimicrobial character due to the presence of both cationic and n-halamine forming structures. in its unchlorinated form, the coating inactivated listeria monocytogenes by ∼3 logarithmic cycles. in the form of ...	2015	25871333
a duplex qpcr for the simultaneous detection of escherichia coli o157:h7 and listeria monocytogenes using lna probes.	in this study, a duplex qpcr assay was developed for the needs of the irish fish industry to screen for the two major food-borne pathogens of fish, listeria monocytogenes and escherichia coli o157:h7. the assay can claim positive or negative results for two pathogens in one go in only 20 h including 16 h universal pre-enrichment and compared to traditional iso approved plate culture methods the labour and the cost involved in testing of one sample is reduced to minimum. the highly specific genom ...	2015	25869996
contrasting regulation of macrophage iron homeostasis in response to infection with listeria monocytogenes depending on localization of bacteria.	due to its multiple roles for the proliferation and pathogenicity of many microbes on the one hand and via modulation of immune effector functions on the other hand the control over iron homeostasis is thought to play a decisive role in the course of infections. diversion of cellular iron traffic is considered as an important defense mechanism of macrophages to reduce metal availability for intracellular bacteria residing in the phagosome. however, evidence is lacking whether such alterations of ...	2015	25869778
development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of listeria monocytogenes.	a real-time loop-mediated isothermal amplification (realamp) assay for the detection of listeria was developed. the realamp assay, using primers specific for the hemolysin-encoding hlya gene, was verified using listeria monocytogenes strains (n = 58) from different regions of the world. both the sensitivity and specificity of the realamp assay were high. the realamp assay could detect 10(3) cfu ml(-1) within 30 min. a comparative evaluation of the realamp assay, the api listeria assay, and the r ...	2015	25868481
cell-autonomous responses in listeria monocytogenes infection.	listeria monocytogenes is a facultative intracellular bacterium causing listeriosis, a food-borne infection with a high mortality rate. the mechanisms and the role of cells and tissular components in generating protective adaptive immune responses are well studied, and cell biological studies provide a detailed understanding of the processes targeted by the bacterial products. much less is known of the cellular responses activated to limit infection in individual cells when confronted with stres ...	2015	25865195
is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?	to evaluate the gut microbial profile in chronic kidney disease (ckd) patients and evaluate the possible relationship with inflammation and cardiovascular risk.	2015	25865191
pulsed-field gel electrophoresis for listeria monocytogenes.	pulsed-field gel electrophoresis (pfge) is a molecular subtyping method with high discriminatory power, reproducibility, and epidemiological concordance for the subtyping of listeria monocytogenes and other bacteria. pfge uses rare-cutting restriction enzymes (macrorestriction) that cut the genomic dna, usually resulting in 6-25 dna fragments ranging between 30 and 600 kb. bacterial cells are immobilized in agarose plugs and subsequently lysed to release genomic dna, which is then subjected to d ...	2015	25862047
pfge as a tool to track listeria monocytogenes in food processing facilities: case studies.	the use of pfge to track listeria monocytogenes strains from the food processing environment to the food is explained in this chapter through two case studies. this illustrates the usefulness of this method to identify putative routes of contamination and persistent strains and to help in the implementation of corrective actions in food processing facilities to control the occurrence of this pathogenic bacterium.	2015	25862045
harmonization of pfge profile analysis by using bioinformatics tools: example of the listeria monocytogenes european union reference laboratory network.	standardization in comparison and interpretation of profiles is an integral part of the analysis of gels from pulsed-field gel electrophoresis. using listeria monocytogenes as an example, this chapter outlines the analytical process using the software bionumerics.	2015	25862044
listeria monocytogenes: a model pathogen to study antigen-specific memory cd8 t cell responses.	memory cd8 t cells play a critical role in providing protection to immune hosts by orchestrating rapid elimination of pathogen-infected cells after re-infection. systemic bacterial infection with listeria monocytogenes has been a favored approach for researchers to characterize pathogen-specific cd8 t cell responses, and in-depth understanding of l. monocytogenes biology has provided invaluable experimental tools that have been used to increase our understanding of memory cd8 t cell differentiat ...	2015	25860798
complete genome sequences of vb_lmos_188 and vb_lmos_293, two bacteriophages with specificity for listeria monocytogenes strains of serotypes 4b and 4e.	listeria monocytogenes is responsible for the rare disease listeriosis, which is associated with the consumption of contaminated food products. we report here the complete genome sequences of vb_lmos_188 and vb_lmos_293, phages isolated from environmental sources and that have host specificity for l. monocytogenes strains of the 4b and 4e serotypes.	2015	25858822
multiplexed detection of foodborne pathogens based on magnetic particles.	this paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: salmonella enterica, escherichia coli o157:h7 and listeria monocytogenes. end-point and real-time pcr, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of ...	2015	25858812