Publications

stage v (fibrotic) allergic bronchopulmonary aspergillosis. a review of 17 cases followed from diagnosis.	a review of the records of 17 patients with stage v (fibrotic stage) allergic bronchopulmonary aspergillosis observed since initial diagnosis (mean observation period, 4.9 years) demonstrated that, of the 11 surviving patients, four have very severe respiratory impairment. the other seven patients have mild or moderate functional impairment, but most of these have not shown clinical deterioration during the observation period. the occurrence of new roentgenographic infiltrates after the time of ...	1987	3545117
synthesis and quantitative structure-activity relationship analysis of n-triiodoallyl- and n-iodopropargylazoles. new antifungal agents.	new series of n-(2,3,3-triiodoallyl) and n-(3-iodopropargyl) azole derivatives (100 compounds) involving pyrrole, pyrazole, imidazole, triazole, and tetrazole nuclei were synthesized successively with the aid of quantitative structure-activity relationship (qsar) analysis to obtain potent antifungal agents. starting from the derivatives of nitropyrrole-containing antibiotics, the qsar analysis of the pyrrole derivatives against candida albicans and trichophyton mentagrophytes strains indicated t ...	1987	3546691
cross-reactivity between antigens of fungal extracts studied by rast inhibition and immunoblot technique.	we have used the rast-inhibition technique in homologous and heterologous inhibitions of the aspergillus fumigatus, alternaria tenuis, and cladosporium herbarum rast assays followed by the immunoblot technique to assess the degree of shared allergenic determinants in these extracts. in the aspergillus rast, there was little or no inhibition with cladosporium and alternaria, but considerable cross-reactivity was found between alternaria and cladosporium. inhibition by alternaria of the cladospori ...	1987	3549841
receptor-mediated clearance of aspergillus galactomannan.	the fate of radiolabeled aspergillus fumigatus galactomannan was studied after intravenous injection into rabbits and rats. at 1 hr, the liver contained 35% of the injected dose in rabbits and 30% in rats. excretion of galactomannan into the urine, measured in rabbits, was another major catabolic route and accounted for 35% of the dose by 24 hr. immunization of rabbits increased hepatic uptake and decreased urinary excretion. hepatic uptake in unimmunized rats could be decreased by saccharomyces ...	1987	3549914
histopathology of organ lesions in mice after an intravenous or intratracheal or intrarenal infection with aspergillus fumigatus.		1987	3550456
tremorgenic mycotoxins from aspergillus fumigatus as a possible occupational health problem in sawmills.	wood-trimmers' disease, generally called extrinsic allergic alveolitis, which affects workers in sawmills, is thought to be caused by fungal diaspores. the importance of aspergillus fumigatus on the surface of wood dried in kilns is accentuated by its ability to produce tremorgenic mycotoxins. eight strains of a. fumigatus from five different sawmills were isolated and cultivated on liquid media, and one of the strains was also cultivated on wood blocks. extracts were prepared, and the tremorgen ...	1987	3555338
microbiological diagnosis of suppurative keratitis in bangladesh.	experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ...	1987	3555609
the role of infection in asthma.	the possible role of bacterial, viral and fungal infections in the development, exacerbation and treatment of asthma are discussed. although bacterial allergy has in the past been advocated as an important etiologic factor for asthma, the evidence is inconclusive. hyposensitization with bacterial antigens is no longer an accepted treatment. bacterial infection of the nasal sinuses and bronchi may exacerbate an asthmatic attack and in some cases patients benefit from antibiotic therapy. however, ...	1987	3556065
allergic bronchopulmonary aspergillosis. model of bronchopulmonary disease with defined serologic, radiologic, pathologic and clinical findings from asthma to fatal destructive lung disease.	allergic bronchopulmonary aspergillosis (abpa) complicates asthma and results in immunologic lung destruction. respiratory failure or fatalities from end-stage fibrotic lung disease have occurred in patients in the third and fourth decades of life. allergic bronchopulmonary aspergillosis may be confirmed in patients with varying severity of asthma from minimal to corticosteroid-dependent and has been reported to occur in approximately 10 percent of patients with cystic fibrosis. it has been docu ...	1987	3556066
aspergillus myositis in a patient with a myelodysplastic syndrome.	we present the case of an elderly man who, while being treated with corticosteroids for a myelodysplastic syndrome, developed myositis of the calf due to aspergillus fumigatus. despite therapy with amphotericin b the myositis failed to resolve and he died. at autopsy, a localized necrotizing myositis of the right calf was found with no evidence of disseminated aspergillus infection. myositis in the setting of disseminated candidiasis or cryptococcosis has been previously reported. this case is u ...	1987	3574431
direct examination vs culture of biopsy specimens for the diagnosis of keratomycosis.	in two patients with fungal keratitis, direct examination of corneal biopsy specimens showed positive fungal elements, but cultures of biopsy specimens failed to disclose fungal growth. we compared the value of direct examination and culture of biopsy specimens in the diagnosis of keratomycosis in rabbits with experimental fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. cultures disclosed seven specimens (70%) positive for candida and eight (80%) for fusa ...	1987	3578461
separation of organic dust from microorganism suspensions by partitioning in aqueous polymer two-phase systems.	conidia of penicillium brevi-compactum and aspergillus fumigatus, sporangiospores of rhizopus rhizopodiformis, spores of streptomyces griseus, and bacterial cells of bacillus subtilis were partitioned in two-phase systems consisting of dextran, polyethylene glycol, substituted positively charged sulfonylpolyethylene glycol, and water. at a ph of 2.8 in the system, the microorganisms showed 60 to 90% affinity for the upper, polyethylene glycol-rich phase, except for cells of b. subtilis, which we ...	1987	3579285
[bronchocentric granulomatosis as a manifestation of allergic bronchopulmonary aspergillosis without bronchial asthma].	a 17-year-old girl developed fever, cough and hemoptysis, as well as bouts of septic fever, dyspnea on exertion, cyanosis and weight loss. opacification in the left lower lung was a persistent feature. complement-fixation reaction to aspergillus fumigatus was 1:80, and there were four precipitation lines against this fungus on immunoelectrophoresis. aspergilli were also demonstrated in sputum and bronchial aspirate. because the patient's condition deteriorated an atypical lingula resection was p ...	1987	3595464
chronic granulomatous disease with cranial fungal osteomyelitis and epidural abscess. case report.	a patient is described with osteomyelitis of the cranium and epidural abscess due to aspergillus fumigatus as the presenting manifestations of chronic granulomatous disease. the diagnosis was suggested by the unusual nature of the organism isolated and confirmed by appropriate laboratory studies. the details of diagnostic assessment and therapeutic management are discussed, and the central nervous system manifestations of chronic granulomatous disease are reviewed.	1987	3598663
evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents.	sewage samples collected from animal wastes and from effluents at an animal disease laboratory were inoculated with known numbers of pathogenic organisms and subjected to various doses of gamma radiation from a 60co source. surviving test organisms were quantitatively determined by selective and enrichment techniques. the experiment was modeled as a quantal assay in which probit analysis was applied to obtain d10 values. the d10 value represents the irradiating dose required to reduce the popula ...	1987	3651881
[bacteriological aspects of 64 cases of severe pneumonia seen in a respiratory intensive care unit].	we report on the bacterial aspects of 64 cases of severe pneumonia in an intensive care over a two year period, excluding cases occurring during artificial ventilation. in all the cases, the grave respiratory and haemodynamic signs, the blood gas and radiological findings justified admission of these patients to an intensive care unit. specimens for bacteriology, virology and parasitology enabled a precise microbiological diagnosis 43 times (63%); in 44% of these diagnosis the possibility of the ...	1987	3671863
[diagnostic bronchoscopy--mycologic and bacteriologic findings].	the authors studied the findings of mycotic and bacterial agents in the lower air passages of 122 patients with lung disease examined by bronchoscopy. the series represents a current profile of patients investigated at the department of tuberculosis and respiratory diseases, pardubice hospital within 17 months of the years 1982-1983. bronchial swabs were compared with the findings in the swabs of the throat taken immediately before the endoscopic examination. yeast organisms were isolated from t ...	1987	3673125
diagnostic specificity of a sandwich elisa for aspergillus-related diseases.	a sandwich elisa with specificity for a major antigen (ag 7) of aspergillus fumigatus has been compared with indirect elisas with use of crude antigenic (culture filtrate) extracts and found to have a sensitivity of 100% and specificity of 92.3% for antibody detection in sera of patients with allergic bronchopulmonary aspergillosis (abpa) and aspergilloma. in the ag 7 elisa, all sera from groups with abpa (21) and aspergilloma (15) had positive titers, and mean values for both these groups were ...	1987	3819216
incidence of keratinophilic fungi in rice-field soils.	soil samples from rice-fields, collected at monthly intervals, were analysed by baiting technique for the incidence of keratinophilic fungi. nine species, among a total of 102 species isolated by various methods, were keratinophilic species (8.8%). these were recorded from 38 out of 45 samples collected. the distribution pattern of the different keratinophilic fungi and their significance are discussed.	1987	2436049
possible confusion of aspergillus fumigatus and pneumocystis carinii.		1987	2449349
galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits.	purified galactomannan (gm) from aspergillus fumigatus was used in both a radioimmunoassay and an enzyme-linked immunoassay for antigen detection. results of the two tests seemed interchangeable. by one or both assays, gm was detected in serum from four of 12 rabbits lethally infected with a. fumigatus in concentrations ranging from 108 to 356 ng/ml. serum antigen was detected in only two of 12 patients with invasive aspergillosis. results of assay for gm in urine were far more encouraging. urin ...	1987	3098861
[amphotericin b and flucytosine therapy of aspergillus pneumonia and acute renal failure].	kidney failure and pneumonia by aspergillus flavus and a. fumigatus were found in a 56-year-old woman who had received antibiotic and corticoid treatment to control high fever. her bloody tracheal secretion was a suspension of granule-like spore-free colonies of both aspergillus species. hemorrhages in mucous membranes and skin suggested a hematogenous dissemination of the fungi. aspergillus spores in the soil of ornamental plants were assumed to be responsible for the inhalatory infection. the ...	1987	3104658
enzyme-linked immuno-filtration assay (elifa) for the detection of igg, igm, iga and ige antibodies against aspergillus fumigatus.	the enzyme-linked immuno-filtration assay (elifa) permits detection of serological precipitating systems preformed by immunoelectro-diffusion on cellulose acetate strips and simultaneous characterization of immunoglobulins g, m, a and e specific for antigens of aspergillus fumigatus. we selected 36 sera from 9 patients who were followed up regularly and who suffered from aspergilloma (5 cases), allergic bronchopulmonary aspergillosis (2 cases), aspergillus bronchitis and invasive aspergillosis ( ...	1987	3110398
botrytis cinerea: a study of the immunological properties during growth. incidence of antibodies against b. cinerea in a group of patients with aspergillosis.	when growing botrytis cinerea in sabouraud medium, different phases of growth could be recognized using ph, mycelium yield, and culture filtrate antigens as parameters of growth. immunological characteristics of the different phases (early, intermediate, and late) of growth were measured using ige and igg binding by enzyme allergosorbent test and enzyme-linked immunosorbent assay, respectively, and quantities of precipitating components (double immunodiffusion and rocket immunoelectrophoresis). ...	1987	3112026
physical and morphological characteristics of eucaryotic ribosomes and lipopolysaccharide complexes.	lipopolysaccharides (lps) from pasteurella multocida or brucella abortus were complexed with aspergillus fumigatus ribosomes by mixing and fixation for 3 days in 3.8% formaldehyde. to investigate the nature of their physical association, ribosomes, lps, and ribosome-lps complexes were (i) centrifuged in cscl gradients to determine buoyant densities, (ii) examined by electron microscopy, and (iii) monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ribosomes were found to bind ...	1987	3114145
effects of endotoxin (lipopolysaccharides) on experimental aspergillosis in leukemic mice.	in an attempt to evaluate effects of bacterial endotoxin on systemic fungal infection, experimental systemic aspergillus infection was induced in leukemic mice (akr strain) with (group b) or without (group a) preceding intraperitoneal administration of pseudomonas aeruginosa derived lipopolysaccharides. all mice in group a died within 4 days after intravenous inoculation of aspergillus fumigatus spores and developed extensive and disseminated fungal lesions without inflammatory reactions. in con ...	1987	3116430
qualitative and quantitative microbiological analysis of sputa of 102 patients with cystic fibrosis.	a microbiological analysis of 102 patients suffering from cystic fibrosis was conducted over a 22 month period. 20 microbial species with the following incidence were identified: pseudomonas aeruginosa: 83.4%; candida albicans: 29.4%; staphylococcus aureus: 24.5%; staphylococcus epidermidis: 11.8%; haemophilus influenzae: 11.8%; streptococcus pneumoniae; 6.9%; pseudomonas maltophilia: 6.8%; aspergillus fumigatus: 5.9%. other species were present in less than 5% of the patients. in the majority o ...	1987	3117700
[solid phase radioimmunoassay for the quantitative determination of antigen-specific igg-antibodies in the serum of patients with farmer's lung].	in this study we have investigated a number of technical aspects of a sensitive solid-phase radioimmunoassay (microtitre-plate ria) for the quantitative determination of antigen-specific igg antibodies in the sera of patients suffering from farmer's lung. microtitre-plates were coated with antigens of aspergillus fumigatus, micropolyspora faeni, aureobasidium pullulans, alternaria tenuis, thermoactinomyces vulgaris and hay. optimal antigen concentration and serum dilution were determined, the te ...	1987	3121779
[effect of temperature and moisture in feed on the growth of aspergillus fumigatus and the formation of gliotoxin].		1987	2471040
participation of cell-mediated immunity in allergic bronchopulmonary aspergillosis.	while ige and igg antibodies are thought to play important pathogenetic roles in allergic bronchopulmonary aspergillosis (aba), the microscopic lung picture would implicate cell-mediated immunity. patients with aba do not manifest delayed skin test reaction to aspergillus fumigatus (af). this has been attributed to inhibition of lymphokines by histamine locally released as a result of the immediate skin test reaction. we tested 5 aba patients for delayed hypersensitivity to af. an attempt was ma ...	1987	2886438
[clinical observations on nasosinus aspergillosis].		1987	2955485
cellular and humoral immune responses in experimental aspergillosis in mice.	invasive aspergillosis was established in a naive murine model. both humoral and cellular aspects of the immunological mechanism responded to invasion. circulating immune complexes containing igg in the infected group of animals were detected by both the c1q and conglutinin solid phase assays. attempts to identify these complexes as aspergillus antigen-specific complexes were unsuccessful. cellular responses, as measured by blastogenic activity and inhibition of migration of sensitized cells wer ...	1987	2957876
aspergillary mycetomas ("aspergillomas") of the lung. histopathologic and mycologic investigations in 45 cases.		1987	2958697
[aspergillus spondylodiscitis].		1987	2962114
value of bronchoalveolar lavage in the management of severe acute pneumonia and interstitial pneumonitis in the immunocompromised child.	the diagnostic value of 73 bronchoalveolar lavages was assessed in 67 immunocompromised children (aged 3 months to 16 years) with pulmonary infiltrates. thirty one children had primary and 19 secondary immune deficiency, 14 acquired immunodeficiency syndrome (aids), and three aids related complex. bronchoalveolar lavage was performed during fibreoptic bronchoscopy, under local anaesthesia in all but two. one or more infective agents was found in eight of 11 patients with severe acute pneumonia a ...	1987	2827334
a comparison of the efficacy of itraconazole, amphotericin b and 5-fluorocytosine in the treatment of aspergillus fumigatus endocarditis in the rabbit.	the efficacy of amphotericin b, 5-fluorocytosine and itraconazole was compared for the treatment of experimental rabbit aspergillus fumigatus endocarditis. therapy with amphotericin b or 5-fluorocytosine, at dosages of 3.0 and 35 mg/kg body weight respectively, failed to eradicate aspergillus from the cardiac vegetations in all but one of the animals tested; none of these animals survived for longer than nine treatment days. when similar doses of amphotericin and 5-fluorocytosine were administer ...	1987	2828301
leukotriene b4 generation by human neutrophils following igg-dependent stimulation.	it has previously been shown that human neutrophils generate substantial quantities of ltb4 when stimulated with the calcium ionophore, a23187, or with unopsonized zymosan. we now report that normal human neutrophils produced substantial quantities of ltb4 (measured by radioimmunoassay and validated by rp-hplc) when incubated with large non-phagocytosable igg-coated beads (sepharose 4b). ltb4 was identified in both the extra and intracellular compartments. the production of ltb4 was dependent up ...	1987	2832306
[cytomegalovirus-associated superinfection of the lung following kidney transplantation].	in a kidney-transplant patient, there was superinfection of the lungs by klebsiella pneumoniae and legionella pneumophilia with multiple necroses in the course of a primary cytomegalovirus infection. in a later phase of the disease, there was an opportunistic colonization of the necrotic cavities and the adjacent lung tissue by aspergillus fumigatus and candida albicans. the cytomegalovirus infection led to a pronounced cellular immunosuppression in the patient. this favored superinfections by b ...	1987	3026768
degenerative changes in fungi after itraconazole treatment.	changes in morphogenetic behavior and structural degeneration after exposure to itraconazole are illustrated in candida albicans, cryptococcus neoformans, pityrosporum ovale, paracoccidioides brasiliensis, trichophyton rubrum, and aspergillus fumigatus. with the exception of p. ovale, primary alterations are seen at the cell periphery and the cytoplasmic vacuoles in which lipid-like vesicles assemble. these changes are usually accompanied by a marked increase in cell volume, impaired cell divisi ...	1987	3027844
early experience with itraconazole in vitro and in patients: pharmacokinetic studies and clinical results.	the efficacy of itraconazole, a new triazole antifungal agent, was studied in vitro and assessed in patients. the mics of itraconazole for 16 strains of aspergillus fumigatus were in the same range as those of amphotericin b: less than 0.09-0.36 microgram/ml vs. less than 0.09-0.78 microgram/ml, respectively. eight adult patients with systemic fungal infections were treated orally with 100-200 mg of itraconazole two times a day. a patient with relapsing histoplasmosis (histoplasma capsulatum var ...	1987	3027850
[aspergillosis following liver transplantation as a hospital infection].	in the first four weeks after a liver transplantation, there was an invasive aspergillosis with a lethal course in three out of five patients who were treated postoperatively in the same room. the clinical symptoms were very different. one patient was asymptomatic, and the diagnosis could only be made by autopsy. in another patient, pulmonary symptoms, and in the third patient, cerebral symptoms were the most prominent. in the two latter patients, the infection was demonstrated in the sputum and ...	1987	3028750
efficacy of fluconazole (uk-49,858) against experimental aspergillosis and cryptococcosis in mice.	the efficacy of fluconazole, a new bis-triazole antifungal agent, was compared with that of orally administered ketoconazole and parenterally administered amphotericin b against aspergillus and cryptococcus infections in mice. fluconazole was 5-20-fold more active than ketoconazole against systemic aspergillosis and against systemic, intracranial and pulmonary cryptococcosis but was less active than amphotericin b.	1987	3038824
suppression of host defences by aspergillus fumigatus.	an important feature of the microbicidal action of phagocytic cells is their ability to produce reactive oxygen intermediates. in an attempt to identify the mechanisms by which the fungus aspergillus fumigatus resists normal host defences the effect of spores and spore diffusates of a fumigatus on the production of superoxide anion and hydrogen peroxide by primed rodent phagocytic cells has been measured. for comparison we have used the non-pathogenic fungus penicillium ochrochloron. production ...	1987	3039682
oxidative killing of aspergillus fumigatus proceeds by parallel myeloperoxidase-dependent and -independent pathways.	the relative importance of several oxygen intermediates in fungicidal action against opsonized aspergillus fumigatus conidia was investigated with monocytes from normal volunteers and patients with either chronic granulomatous disease or myeloperoxidase (mpo) deficiency. results from experiments in which catalase, taurine, mannitol, or glucose-glucose oxidase were added to these phagocytes indicated that the mpo-hydrogen peroxide-halide system and an mpo-independent oxidative system exerted comp ...	1987	3040589
activation of human serum complement with allergens. i. generation of c3a, c4a, and c5a and induction of human neutrophil aggregation.	to understand the relevance of allergens in generation of c3a, c4a, and c5a in normal human serum, we studied extracts of several allergens (house dust, house dust mite, aspergillus fumigatus, and perennial ryegrass). known complement activators zymosan and endotoxin were used as controls. generation of c3a, c4a, and c5a (determined by radioimmunoassays) occurred with extracts of house dust and aspergillus more than with house dust mite and ryegrass. anaphylatoxins were produced both in dose and ...	1987	3496373
serum precipitins against microbes in mouldy hay with respect to age, sex, atopy, and smoking of farmers.	this study was based on a sample of 3,065 farmers from a larger survey population of 12,056 finnish farmers. data were collected in a postal survey conducted by the social insurance institution of finland. serum samples for determination of precipitating antibodies were taken at local health centres. precipitins were determined by the method of microplate immune diffusion. the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, ...	1987	3499339
serum precipitins against microbes in mouldy hay with respect to the geographical location of the farm and to the work of farmers.	this study was based on a sample of 3,065 farmers from a larger survey population of 12,056 finnish farmers. data were gathered in a postal survey conducted by the social insurance institution of finland. serum precipitins were determined by a microplate method of immune diffusion. the antigen panel consisted of mycelial antigens of micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and aspergillus umbrosus. geographical variation in the prevalence of precipitins was statis ...	1987	3499340
distribution of the genus aspergillus in hospital room air conditioners.		1987	3123413
detection of galactomannan antigenemia by enzyme immunoassay in experimental invasive aspergillosis.	a sensitive enzyme immunoassay (eia) for galactomannan antigenemia that avoids the use of radioisotopes was devised. three carbohydrate-rich antigenic fractions were purified from aspergillus fumigatus 2085: a cold alkali extract (ca) from mycelium, an acetone-precipitated pyridine extract (apsk-66) from mycelium, and a methanol precipitate from culture filtrate. ca and apsk-66 were further purified by gel filtration and ion-exchange chromatography, respectively. an acid hydrolysate of ca contai ...	1987	3294887
fungal infections in birds in captivity--six case reports.		1987	3295539
fatal hemoptysis in a patient with aids-related complex and pulmonary aspergilloma.		1987	3295611
the synchronous occurrence of aspergillosis and myospherulosis of the maxillary sinus. report of a case with review of the literature.		1987	3295652
rapid diagnosis of candidiasis and aspergillosis.	published studies support the hypothesis that at least two antigens of candida albicans and candida tropicalis circulate in the bloodstream of patients with severe candidiasis. one antigen (probably mannan) is stable and the other (probably protein) is labile. the stabile antigen can rarely be detected without prior dissociation from antibody. dissociation treatment destroys the labile antigen, leaving in doubt whether any is antibody bound. dissociation steps have also been necessary for detect ...	1987	3296104
[aspergillosis in wild geese (chloëphaga poliocephala scl., 1857) in chile].		1987	3296570
[allergic bronchopulmonary aspergillosis].	the article concerns one of the present-day problems in the teaching of the allergic diseases of the lungs, namely allergic bronchopulmonary aspergillosis. discusses the disease diagnosis and clinical course, diagnostic approaches, efficient drug therapy, and prognostic aspects. based on an analysis of the authors' own data, the problem of the possibility of the systemic pathoimmune manifestations associated with skin, eye and kidney injuries that occur during allergic bronchopulmonary aspergill ...	1987	3299854
quantitative neutralization assay of fungicidal activity of disinfectants.	a quantitative assay using a neutralization medium was developed to evaluate fungicidal activity of disinfectants. concentrated dey-engley neutralizing broth was used in this study and was demonstrated to inactivate various chemical agents within 5 min when disinfectant concentrations were reduced to specific levels. addition of this dey-engley broth to test tubes containing fungal cells and disinfectants permitted control of the various interactions times. subsequent concentration of the disinf ...	1987	3300541
inhibition of germ tube development of aspergillus fumigatus in cell-free transudate produced in subcutaneous chambers in rabbits.	the interaction of aspergillus fumigatus conidia with host factors produced in rabbits was studied by means of subcutaneous, perforated plastic chambers. transudate fluid from spore-free chambers, sampled 30 days after implantation, supported germ tube development and rapid hyphal growth of a. fumigatus in an assay in vitro. inoculation of spores into chambers implanted 30 days previously produced a rapid infiltration of leukocytes, predominately neutrophils, into the chamber fluid. cell-free su ...	1987	3302197
synthesis and antifungal activity of a series of novel 1,2-disubstituted propenones.	to find an antifungal agent other than those of the imidazole and triazole series, a new class of 1,2-disubstituted propenones i and ii was prepared and tested for antifungal activity. comparison of the structure-activity relationships showed that the conjugated structure of carbonyl and exomethylene groups in i and ii plays an important role in potent antifungal activity. however, it is noteworthy that compounds 53, 54, and 56, which have a hydroxymethyl or methoxymethyl group instead of an exo ...	1987	3302258
invasive bronchopulmonary aspergillosis leading to rapidly progressive respiratory failure in a patient with severe aplastic anemia.	a 22-year-old man with severe aplastic anemia was treated with antilymphocyte globulin, prednisone, and oxymetholone. fourteen days after initiation of treatment he developed a fulminant mediastinal and subcutaneous emphysema leading to respiratory failure refractory to mechanical ventilation. fiberoptic bronchoscopy showed nodular lesions typical of aspergillus. cultures of bronchial mucus revealed aspergillus fumigatus as the responsible pathogen.	1987	3302517
an outbreak of invasive aspergillosis in a haematologic unit.	within a period of 15 months 8 cases of invasive pulmonary aspergillosis were seen in a haematologic unit; 7 of them between january and october 1984. no previous cases of invasive aspergillosis had been encountered during the existence of the unit since 1979. environmental studies did not prove a single likely source for the fungal spores. previous window renovation with concomitant fiber deposits on ventilation grids, poor sealing of air filter fittings in patient rooms, occasional ventilation ...	1987	3303306
trial of ketoconazole in non-invasive pulmonary aspergillosis.	a one year study of the efficacy of the antifungal agent ketoconazole in non-invasive pulmonary aspergillosis was carried out. ten patients, seven with allergic bronchopulmonary aspergillosis and three with mycetoma, were studied. they were randomly allocated to receive 400 mg daily or placebo orally in a double blind fashion. in the treated group (n = 6), concentrations of serum igg specific for aspergillus fumigatus fell significantly during treatment (mean reduction 42% (sem 2.2%) compared wi ...	1987	3303424
fumifungin, a new antifungal antibiotic from aspergillus fumigatus fresenius 1863.		1987	3305454
right occipital cerebral abscess caused by aspergillus fumigatus.	a rare case of intracerebral abscess caused by aspergillus fumigatus located in the right occipital region is described; pathogenesis, prognosis and treatment of this particular infection are discussed.	1987	3305803
[diagnosis of invasive pulmonary aspergillosis].		1987	3306195
pleural aspergillosis diagnosed by computerized tomography.	as shown by the case we have reported, patients with a chronic pneumothorax and nodular, thickened pleura may have pleural aspergillosis. we recommend that when such patients have ct of the chest, the examiner use the alternate dependent position to look for mobile pleural debris.	1987	3306949
release of antigens and allergens during shake-culture of aspergillus fumigatus.	the appearance of igg and ige binding components in the medium of shake cultures of aspergillus fumigatus has been studied. cultures were grown in synthetic asparagine medium at 35 degrees c and flasks harvested in duplicate 1, 2, 3, 4, 7 and 14 days after inoculation. the ph of the medium dropped from its initial value of 5.5 to ph 3, and then after 4 days gradually increased up to ph 7.5 in the 14-day medium. the weight of mycelium, after an initial peak followed by a slight decline, increased ...	1987	3307516
disseminated aspergillosis in a patient with ocular reticulum cell sarcoma.	a 75-year-old healthy woman developed uveitis and chorioretinal infiltrates od compatible with reticulum cell sarcoma. she was admitted to hospital 18 months later with somnolence and confusion. examination revealed diffuse white matter hypodensity on computed tomography and a persistent cerebrospinal fluid pleocytosis. at necropsy reticulum cell sarcoma was found in the right globe and aspergillus fumigatus was present in almost every other organ, including the brain.	1987	3307899
resistance of spores of aspergillus fumigatus to ingestion by phagocytic cells.	phagocytic cells are believed to have an important role in the eradication of fungal spores from the lung. the ability of human and mouse cells to phagocytose the opportunistic fungus aspergillus fumigatus has been examined, spores of the non-pathogenic fungus penicillium ochrochloron being used for comparison. most spores became associated with cells. those of a fumigatus appeared to remain bound to the surface of the phagocyte rather than being ingested; in contrast, p ochrochloron spores appe ...	1987	3310309
invasive pulmonary aspergillosis diagnosed by blood culture and successfully treated.	we present a case of invasive pulmonary aspergillosis when a blood culture isolate of aspergillus fumigatus in an unusual medium was considered to be a genuine isolation. early institution of appropriate therapy was successful.	1987	3311121
immune responses in experimental keratitis by aspergillus fumigatus fresenius.		1987	3311979
serologic response to candida albicans and aspergillus fumigatus in cystic fibrosis.	in order to evaluate the role of fungi in patients with cystic fibrosis, we determined serum igg antibodies against candida albicans and aspergillus fumigatus using an indirect elisa in patients with or without fungi in the sputum and in sera of healthy individuals. for both c. albicans and a. fumigatus the antibody levels were significantly higher in the cf groups than in the control group, regardless of whether these fungi could be isolated during the observation time or not. in contrast to a. ...	1987	3312024
electrophoretic and serological analyses of cytoplasmic antigens from aspergillus fumigatus during growth of conidia to mature mycelia.	the changes of cytoplasmic components concomitant with conidium to mature mycelium growth of aspergillus fumigatus strain ag 507 were analysed by one- and two-dimensional polyacrylamide gel electrophoresis (sds-page; 2-de). sds-page monitored molecular weight differences between components of cytosol preparations obtained from conidia and those through 96 h of mycelial growth. 2-de analyses indicated that some components characteristic of mature cytosol begin to appear by 7 h. cytoplasmic prepar ...	1987	3312558
[aspergillus pneumonia in patients with chronic bronchopathy. presentation of 3 cases].		1987	3312858
isolated cardiac aspergillosis after bone marrow transplantation.	a 15-year-old girl developed isolated cardiac aspergillosis after allogeneic bone marrow transplantation. predisposing factors included severe graft-vs-host disease with gastrointestinal tract involvement and immunosuppressive therapy including cyclosporine. the patient died of cardiopulmonary failure 11 weeks after transplantation.	1987	3314764
prolonged evaluation of patients with corticosteroid-dependent asthma stage of allergic bronchopulmonary aspergillosis.	eight cases with stage iv allergic bronchopulmonary aspergillosis (abpa) (corticosteroid-dependent asthma stage) were observed for a total of 82 patient years with individual patients observed for 7 to 19 years (mean 10.2) years. one case is the first case of abpa diagnosed in the united states in 1967. a second case has been observed through four stages of abpa. none of these eight cases has demonstrated pulmonary deterioration by clinical, chest roentgenogram, or pulmonary function analysis. a ...	1987	3316345
[endocarditis caused by aspergillus fumigatus].		1987	3316873
invasive pulmonary aspergillosis: radiologic evidence of tracheal involvement.	chest radiographs of invasive pulmonary aspergillosis in immunocompromised patients typically show ill-defined pulmonary nodules. direct tracheal involvement has not been previously documented radiologically. radiographs and ct scans of a 16-year-old boy with aplastic anemia and invasive pulmonary aspergillosis showed increased paratracheal opacity, tracheal narrowing, and pneumomediastinum in combination with pulmonary disease. bronchial lavage cultures grew aspergillus fumigatus, and the boy d ...	1987	3317503
chip pile workers and mould exposure. a preliminary clinical and hygienic survey.	chip pile workers are exposed to high concentrations of airborne mould spores. few sporadic cases of allergic alveolitis have been reported in finland. eight non-smoking workers, six on chip piles and two on bark piles, were examined to study the immunological and clinical response to continuous mould spore exposure. the spore exposure ranged between 10(4) and 10(5) spores/m3. three of the six chip pile workers had precipitating serum antibodies against aspergillus fumigatus. the three antibody- ...	1987	3318540
characterization of a second major antigen ag 13 (antigen c) of aspergillus fumigatus and investigation of its immunological reactivity.	a second major antigen of aspergillus fumigatus (ag 13) has been identified and characterized as a 70 kd, heat-labile component which is able to bind to the lectin, concanavalin a. ag 13 proved to be identical to the previously recognized c antigen, known to possess chymotryptic activity. a monospecific antiserum to ag 13 has been affinity purified and used to develop a sandwich elisa for measuring levels of ag 13 specific igg antibodies in patients' sera. although all abpa sera (25/25) had sign ...	1987	3319301
invasive pulmonary aspergillosis: a diagnostic and therapeutic problem. clinical experience with eight haematologic patients.	eight patients with haematologic malignancies contracted fatal invasive aspergillosis during an outbreak. five patients were neutropenic. bronchofiberoscopic examination with microbiology specimen brush and bronchoalveolar lavage yielded aspergillus fumigatus in only 2/5 patients examined. the specific diagnosis reached during lifetime in 5 patients was based on a combination of invasive procedures (lung biopsy in 2, percutaneous lung puncture in 1), the presence of a lung abscess (3 patients), ...	1987	3321414
pathogenetic and serological aspects of pulmonary aspergillosis.	af is an important pathogen of the bronchopulmonary system, and the clinical spectrum encompasses aspergilloma, cnpa, ipa, abpa, bronchial asthma, and allergic alveolitis. bronchial carriage may, however, not always be associated with pathological effects. the polymorphism of the aspergillus-related disorders seems mostly to depend upon the different responses of the hosts. this review considers the antigenic composition of af and specific antibody responses in man in relation to the pathogenesi ...	1987	3321416
hematogenously acquired aspergillus vertebral osteomyelitis in seemingly immunocompetent drug addicts.		1987	3321709
evaluation of a test to detect circulating aspergillus fumigatus antigen in a survey of immunocompromised patients with proven or suspected invasive disease.	an elisa for the detection and measurement of aspergillus antigenaemia has been developed and evaluated by examining sera submitted over a 12-month period from immunocompromised patients with a likelihood of invasive aspergillosis. results from proven cases of invasive aspergillosis confirmed at post-mortem and specimens from individuals with suspected disease showed that tests on single serum samples were often negative. multiple specimens from the same patient greatly increased the frequency o ...	1987	3325629
inhibition of phagocyte migration and spreading by spore diffusates of aspergillus fumigatus.	previous studies have shown that spores of aspergillus fumigatus inhibit phagocytosis and killing by macrophages and polymorphonuclear leucocytes. in order to identify the mechanisms of this interference with host defences, we have examined the effects of a. fumigatus spore diffusates on phagocytic cell function. for comparison, we have used spore diffusates of the non-pathogenic fungus penicillium ochrochloron. the diffusates of a. fumigatus reduced the number of human polymorphonuclear leukocy ...	1987	3325630
aspergillosis of the brain: report of an autopsy proven case.		1987	3327911
pulmonary berylliosis on corticosteroid therapy, with cavitating lung lesions and aspergillomata--report on a fatal case.	a fatal case of pulmonary berylliosis in a 42 year old male is described. the patient was exposed to beryllium while working in a chemical plant over a 9 year period, and presented two years after ceasing such employment. the berylliosis was diagnosed on open lung biopsy in 1971. the patient was commenced on steriod therapy at that time. he suffered progressive dyspnoea from severe restrictive lung disease over the next 14 years. a chest x-ray of june 1985 revealed a lesion in the left upper lob ...	1987	3328191
biochemical and immunochemical analyses of detergent solubilized antigens from membrane vesicles of aspergillus fumigatus.	a membrane vesicle fraction isolated from exponentially growing aspergillus fumigatus strain ag 507 cultures was obtained by mechanical disruption of intact aspergillus cells under specific osmotic conditions followed by a ph fractionation technique. electron micrographs of the membrane vesicles indicated unit membrane structures free from cell wall material. high glucose-6-phosphatase and low lactate dehydrogenase activities verified the relative purity of the membrane vesicle fraction. allergi ...	1987	3329002
a case of aspergillosis of the maxillary sinus.		1987	3329218
[allergic bronchopulmonary aspergillosis].		1987	3330331
[studies on the mechanism of developing bronchial asthma due to candida albicans. 5. a comparative study of the mechanisms producing bronchial asthma among candida, other molds and house dust-induced asthma].		1987	3330418
[methods of inducing experimental corneal mycosis].		1987	3330743
[pathogenic properties of aspergillus fumigatus fres].		1987	3334261
[experimental fumigatotoxicosis in mink].		1987	3334269
fungal mastoiditis in the immunocompromised host.	an immunocompromised patient is subject to unusual, severe opportunistic infections. we report a case of aspergillus fumigatus mastoiditis in a patient with leukemia who also had cryptococcal meningitis. a fatal outcome ensued, despite extensive surgical and antimicrobial intervention.	1988	3337779
allergic asthma in different population groups in the western cape. causative and complicating factors.	allergic asthma is a disease with a well-defined aetiology, the recognition and elimination of which could be achieved with relatively simple and inexpensive prophylactic treatment. some of the well-known factors--respiratory tract infections, exposure to cigarette smoke, specific antigens and regular application of prophylactic treatment--which could cause or complicate asthma were studied in groups of white and coloured patients. more respiratory tract infections occurred in coloured patients ...	1988	3340935
skin testing with extracts of fungal species derived from the homes of allergy clinic patients in toronto, canada.	airborne fungi in the homes of patients with allergic rhinitis or asthma, from a toronto allergy clinic population, were isolated, quantified and identified to species. allergen extracts were prepared from sixteen of these isolated species and used for skin-prick testing of twenty-six patients. fourteen of the total patients reacted to one or more of these extracts at 1:10 (w/v) concentrations. the most common positive skin responses (8/14 to 6/14) were found for cladosporium cladosporioides, al ...	1988	3349592
microbiology of indwelling central intravascular catheters.	indwelling central-line catheters (n = 502) from 362 patients in intensive care units were analyzed prospectively. the skin site, 6-cm-distal and -proximal subcutaneous segments of the catheter, exudates, and blood were cultured. semiqualitative roll plate cultures of the catheter segments were followed by broth cultures and examined for 72 h. all isolates were identified, and susceptibilities were determined. line infections, defined clinically, yielded 22 different microbial species; 10 differ ...	1988	3366864
aspergillus rhinitis in wistar (crl:(wi)br) rats.	in two separate 24 month studies on the carcinogenic effect of single cadmium chloride injections in male wistar (crl:(wi)br) rats, a total of 22% (129/597) of animals studied histologically were found to have chronic suppurative rhinitis caused by aspergillus fumigatus. the diagnosis was based on characteristic conidial heads present in the sections, and positive methenamine-grocott (gms) staining of septate hyphae with dichotomous branching at angles of 45 degrees. fungal hyphae balls, surroun ...	1988	3374092
immune responses to aspergillus in cystic fibrosis.	aspergillus fumigatus (af) is well recognized in its ability to colonize the respiratory tract in cystic fibrosis (cf). furthermore, a number of the immune responses of the patient with cf to this organism have been characterized, and the immune inflammatory response to af may result in allergic bronchopulmonary aspergillosis (abpa). this study evaluated a series of immunologic parameters in 75 patients with cf in order to characterize more fully the spectrum of immune responses of those patient ...	1988	3392373
disseminated strongyloides stercoralis and aspergillus fumigatus presenting as diffuse interstitial pneumonitis in a steroid-dependent chronic obstructive pulmonary disease patient.		1988	3397296